12 results about "Glycated protein" patented technology

Discloses herein are conjugates of a protein and one or more reducing sugars, wherein one, two, three or more target amino groups of the protein are glycated. Also disclosed are methods for the preparation of the glycated protein. Conjugates having one or more ketoamine linkages are efficiently prepared through the reaction between the protein and reducing sugars and exhibit desirable pharmaceutical properties.

To provide a glycated protein sensor, a glycated protein measurement method, and a method for producing a glycated protein sensor, which enable high-accuracy measurement and rapid measurement.SOLUTION: Provided is a glycated protein sensor comprising immobilized protease, immobilized ketoamine oxidase, and a hydrogen peroxide detection section.SELECTED DRAWING: None

For a glycated protein sensor provided with an immobilized enzyme, a technique for improving the accuracy of measurement and the stability of the glycated protein sensor is essential. However, an enzyme capable of improving the accuracy and stability of measurement using a glycated protein sensor is still unknown. Provided is a fructosyl amino acid oxidase containing an amino acid sequence in which any amino acid corresponding to the position selected from the group consisting of position 5, position 58, position 225, position 277 and position 440 in the amino acid sequence represented by SEQ ID NO: 1 is substituted when compared with the amino acid sequence represented by SEQ ID NO: 1.

The application discloses a series of glycated hemoglobin specific elimination systems, a preparation method and application thereof. The system is an AIE fluorescent positioning group as a skeleton anchor point, and orderly polymerization of the system with a disulfide bond, a diselenide bond, a ditelluride bond and other stimulus response groups is realized through a Schiff base reaction, so that a series of novel oxidative stress response covalent organic framework materials are constructed, and the excellent self-assembly performance of a hydrazine bond PEG group is utilized to identify the glycated protein capture potential. The series of glycated hemoglobin specific elimination systems have a myocardial targeting property, a long residence time, a significant glycated hemoglobin self-capture system, realize stable and long-acting elimination of a disease course marker glycated hemoglobin and specific targeting regulation of myocarditis inflammatory microenvironment macrophages, provide a new idea and a new tool for targeted treatment of a complex pathogenesis of diabetic cardiomyopathy, and have good research significance and excellent clinical transformation value.

To provide a glycated protein sensor that allows for precise and rapid measurements.SOLUTION: Provided is a glycated protein sensor provided with: immobilized protease; immobilized ketoamine oxidase; and a hydrogen peroxide detector.SELECTED DRAWING: None

The invention relates to the technical field of in-vitro detection reagents, in particular to a preparation method of a glycated protein control product, the glycated protein control product and application of the glycated protein control product. By improving the preparation process of the glycated protein control product, removing free glucose after saccharification incubation and combining a secondary incubation process, unstable intermediates in the protein glycosylation process are reduced, and the quality of the glycated protein control product is improved. The stability of the glycated protein quality control product is improved, excessive functional components do not need to be added, and the method can be suitable for different detection methodology platforms.

In order to analyze a saccharified molecular target and a function regulation network thereof and recognize saccharified proteins and an interaction group thereof, the invention discloses a method for researching interaction of the saccharified proteins on the surface of a cell based on a photocatalytic proximity labeling technology. The method comprises the following steps: firstly, accurately introducing Ru (bpy) 32 < + >-DBCO onto glycated protein on the surface of a cell by utilizing a biological orthogonal method, and combining with 6AzGlc metabolized by non-natural sugar; then, blue light is used for irradiating local parts of the cells, * Ru (bpy) 3 < 2 + > encounters O2 in a ground state and is quenched through energy transfer, < 1 > O2 generated by reaction further oxidizes amino acid residues on the surfaces of the cells, and a biotin-hydrazide probe is used for marking glycated protein and interaction groups of the glycated protein. The introduced proximity labeling mechanism has the advantages of high selectivity, high biocompatibility and no obvious cytotoxicity, the photocatalytic proximity labeling system can be flexibly controlled to be turned on / off by an external light source, and high time resolution is provided for potential dynamic research.

Provided is a glycated protein sensor provided with an immobilized protease, an immobilized ketoamine oxidase, and a hydrogen peroxide detection unit.

This application discloses a predictive model, method, and system for cardiovascular complications in peritoneal dialysis patients based on glycated proteomics and machine learning. This application utilizes techniques such as glycated peptide enrichment, high-throughput glycated proteomics detection, targeted absolute quantification, and machine learning modeling to construct a predictive model suitable for assessing the risk of CVD complications in peritoneal dialysis patients. This model organically integrates glycated peptide biomarkers with clinical indicators, enabling early identification and stratified management of cardiovascular risk in peritoneal dialysis patients, and has good clinical application potential.

Title: Method of detection of glycated proteins in biological samples and use thereof The present invention relates to a method of detection of glycated proteins, particularly gHSA, which comprises the steps of: i) providing anthracene boronic acid methacrylate (ABAM): 10-[N-methyl-N-(o- boronobenzyl)aminomethyl]-anthracene-9-methyl methacrylate; ii) preparing a solution of ABAM in a biocompatible buffer with pH in the range of from 6.5 to 8.5 or in in vitro blood plasma sample of a healthy subject; iii) measuring initial fluorescence intensity of ABAM solution from step ii) at the wavelength of from 410 to 520 nm; iv) in vitro mixing of a known amount of ABAM solution from step ii) with a known amount of biological sample; v) measuring fluorescence intensity of the mixture from step iv) at the wavelength of from 410 to 520 nm; vi) determining the fluorescence intensity increases between the initial control fluorescence intensity measured in step iii) and the fluorescence intensity of the mixture of ABAM and biological sample measured in step v); vii) calculating the amount of glycated proteins in the biological sample. The present invention further relates to the use of this method in determination of the total concentrations of glycated proteins, particularly gHSA, in plasma samples and diagnostics for diabetes mellitus.

The present invention belongs to the field of protein detection and separation materials, and specifically relates to monodisperse polystyrene microspheres for glycated hemoglobin detection and a preparation method thereof. The present invention grafts hyperbranched polyglycidyl ether onto the surface of monodisperse porous polystyrene microspheres, and wraps HPG of a certain molecular weight on the surface of the microspheres by a relatively simple modification method, which can shield the hydrophobic effect on the surface of the microspheres, thereby reducing the non-specific adsorption of proteins, and also improves the biocompatibility and service life of the microspheres, and then grafts olefin chlorides, and finally sulfopropyl monomers and hydrophilic uncharged olefin monomers are surface-modified and polymerized on the HPG surface, which can form a reasonable distribution of sulfopropyl groups and uncharged hydrophilic groups. This structure separates various glycated protein components through efficient ion exchange, thereby making up for the shortcomings of poor separation effect, short service life and high cost of the current modification methods.

According to an embodiment of the present disclosure, a medium with which blood plasma is separated and stored, the medium including a porous medium for separation of blood plasma and a substance supported on the porous medium, the substance that suppresses glycation of a protein by glucose, is provided.