105 results about "Dinitrophenyl" patented technology

An organic luminescent material including a compound of the following formula: wherein: R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11 and R12 are individual groups, and at least one group is not hydrogen among the R1, R3, R7, and R9 groups. Group 1: hydrogen, or alkyl of from 1 to 48 carbon atoms, and each R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11 and R12 can connect with their neighboring group to form 5 or 6 member cyclic or aromatic ring system, and Group 2: aryl or substituted aryl of from 5 to 48 carbon atoms, or 4 to 48 carbon atoms necessary to complete a fused aromatic ring of naphthenyl, anthracenyl, pyrenyl, or perylenyl; and Group 3: heteroaryl or substituted heteroaryl of from 5 to 24 carbon atoms, or 4 to 48 carbon atoms necessary to complete a fused heteroaromatic ring of furyl, thienyl, pyridyl, quinolinyl and other heterocyclic systems; and Group 4: alkoxyl, amino, alkyl amino, aryl amino dialkyl amino, or diaryl amino of from 1 to 24 carbon atoms; and Group 5: a group consist of F, Cl, Br, I, CN, NCS, NCO, B(OH)2, B(OCH2CH2O), B[OC(CH3)2C(CH3)2O], SO2R13, SO3R14, SO2NR2, SiR3, SiHR2, SiR2OH, where R, R13 and R14 is hydrogen, chlorine, bromine, alkyl group containing 1-12 carbon atoms, and aryl; and Group 6: a group of formula -LYnR15 where n is 0 to 18, Y is a alkyl group contains 1 to 24 carbon atoms, R15 is a hydrogen, hydroxy, amino, alkylamino, arylamino, alkyl arylamino, diarylamino, dialkylamino, or —COR16 where R16 is a hydrogen, chlorine, COCl, alkyl group containing 1-12 carbon atoms, —NR2, —NHR and aryl, or —COOR17 where R17 is a hydrogen, alkyl group containing 1-12 carbon atoms, aryl, COR, 2,4-dinitrophenyl, N-imido or —NR2; and L is a direct bond or C═O.

The invention discloses a disperse dye composition, which consists of an original dye and an aid, wherein the original dye consists of any 2-4 kinds of dyes selected from 0-2 kinds of dyes shown as a general formula (I), 0-2 kinds of dyes shown as a general formula (II), 0-2 kinds of dyes shown as a general formula (III) and 0-2 kinds of dyes shown as a general formula (IV), wherein R1, R4, R6 and R8 in the formula (I), formula (II), formula (III) and formula (IV) are independently hydrogen, methyl or methoxy; when R2 is hydrogen, R3 is 2-nitrobenzophenone or 2,4-dinitrophenyl; when R2 is methyl, ethide or cyanoethyl, R3 is benzyl or 2-methyl benzyl; R5 and R7 are independently hydrogen, nitryl, methyl or ethyl; and R9 is methyl, ethyl, benzyl, 2-methyl benzyl or 2-nitrobenzophenone. The invention further discloses a dyeing method for a dacron. The disperse dye composition provided by the invention has the advantages of high dispersing property and high oxygen resistance.

The invention provides a method for separating and measuring acetylcysteine enantiomers. In the method, before acetylcysteine is added in a chromatographic column, a derivatization reagent is used to perform derivatization, wherein the derivatization reagent is N(alpha)-(5-fluoro-2,4-dinitrophenyl)-L-amino acid compound. The method for separation and measurement combines the high performance liquid chromatography (HPLC) or high performance liquid chromatography-mass spectrum (HPLC-MS), and the used chromatographic column uses octadecylsilane chemically bonded silica as filler. The method for separation and measurement comprises the following steps: (1) taking acetylcysteine or a preparation with acetylcysteine, dissolving in low-concentration acid solution, adjusting the pH value of the mixed solution to 6.0-8.0 with alkaline solution to obtain a sample solution for testing; (2) mixing the acetylcysteine solution with 1mol / L of carbonate solution, adding N(alpha)-(5-fluoro-2,4-dinitrophenyl)-L-amino acid compound to mix evenly; (3) reacting the solution obtained by the step (2) at 40-60 DEG C in a dark place, adding hydrochloric acid solution after the reaction; (4) adding the solution obtained by the step (3) in a drying solution with the prestored phosphorus pentoxide and potassium hydroxide, adding phosphate buffer solution-acetonitrile, dissolving residues through ultrasonic treatment, filtering to obtain filtrate which is used as a testing solution; and (5) adopting HPLC or HPLC-MS to separate and measure the testing solution prepared by the step (4).

The invention belongs to analytical chemistry region, and relates to the method of measuring chiral isomer of by high efficiency liquid chromatography after pregabalin deriving. The method of precolumn derivation supplied by the invention has advantages of geniality reaction conditions, steady derivation offspring, fast reaction rate, little subsidiary reaction, etc. It could realize separation and measuration for pregabalin and chiral isomer containing pregabalin preparation.

A method of producing a protein degradation product is provided, by which a protein (including a peptide) in a sample can be degraded quickly and efficiently. A sample containing a protein is treated with a protease in the presence of the tetrazolium compound to give a protein degradation product. Further, by causing a redox reaction between the glycation site of a glycated protein degradation product obtained by this method and a fructosyl amino acid oxidase, and then determining this redox reaction, it is possible to determine the amount of a glycated protein quickly. As the tetrazolium compound, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt etc. can be used.

The invention discloses N-(2,4-dinitrophenyl)-rhodamine B hydrazide and a preparation method and an application of the N-(2,4-dinitrophenyl)-rhodamine B hydrazide. The rhodamine B derivative provided by the invention is N-(2,4-dinitrophenyl)-rhodamine B hydrazide, the structural formula of which is I. The N-(2,4-dinitrophenyl)-rhodamine B hydrazide, is colorless and free from fluorescence, but can selectively react with Cu<2+> ions in chromogenic and fluorescent opening reaction. The colorless substance generates a system with excellent optical performance, so that the detection sensitivity and selectivity of Cu<2+> ions are greatly improved. Therefore, the N-(2,4-dinitrophenyl)-rhodamine B hydrazide is appropriate for high selectivity and high sensitivity detection for the Cu<2+> ions. Detection can be carried out by a light absorption spectrum and a fluorescence spectrum. (FORMULA I).

The invention provides a novel method for preparing an aromatic amine compound through halogenated aromatic hydrocarbon and an organic amine zinc chloride complex compound. A reaction general formula is as shown in a figure in the specification, wherein Ar is aryl and can be selected from 2,4-dinitrophenyl, p-nitrophenyl, 2-nitro-4-chlorphenyl, p-trifluoromethylphenyl, 4-formaldehyde phenyl, 2-pyrimidyl, and the like; X can be selected from F, Cl, Br, I, and the like; R1 and R2 are H, alkyl, aryl or heteroaryl, or R1 and R2 form a ternary to heptatomic naphthenic base together with carbon atoms connected with R1 and R2; reaction media are methylbenzene, dimethyl sulfoxide, N,N-dimethylformamide, tetrahydrofuran, dioxane, acetone and water; reacted alkalis are cesium carbonate, potassium phosphate, triethylamine, potassium carbonate, sodium (potassium) hydroxide and sodium (potassium) alkoxide; reaction can be implemented through both conventional heating and microwave promotion; and the means of recrystallization or column chromatography is adopted for purification. The invention provides a low-cost non-toxic efficient green method (without ligands) for establishing an aromatic C-N bond.

The invention discloses a sialic acid bonded with antigens and application of the sialic acid and relates to sialic acids. The sialic acid bonded with the antigens is composed of 2,4-dinitrobenzene and a sialic acid body. The molecular formula is C17H22N4O12. The molecular weight is 474.1234. The sialic acid bonded with the antigens can be applied to preparation of drugs for immunity prevention and treatment of tumors. After being absorbed by tumor cells, the sialic acid bonded with the antigens is expressed on the surfaces of the tumor cells in a covalency mode through a metabolic pathway. DNP-Sia is intravenously injected to a mouse body having subcutaneous tumors and is fast and effectively expressed on the surfaces of the tumor cells of solid tumors. The tumor cells where the DNP-Sia is expressed can effectively collect DNP antibodies or T cells with cytotoxicity and are combined with the DNP antibodies or the T cells with cytotoxicity, and therefore the tumor cells can be dead. Relevant antigens on the surfaces of the tumors are expanded, and growth of the tumors can be effectively regulated and controlled.

The invention discloses an ESIPT (excited state intramolecular proton transfer) type fluorescent probe for biological mercaptan detection. The ESIPT type fluorescent probe is 2-(4-(2,4-dinitrophenyl sulfonyloxy)-3-formylphenyl)-4-methylthiazole-5-ethyl carboxylate, wherein 2,4-dinitrophenyl sulfonyl serves as a recognition group, and 2-(3-formyl-4-hydroxyphenyl)-4-methylthiazole-2-ethyl formate serves as an information reporter group. A preparation method of the ESIPT type fluorescent probe is simple, the fluorescent probe can enter cells simply and rapidly and specifically bound with biological mercaptan in the cells, so that the fluorescent probe has an obvious fluorescence enhancement effect, can realize distinguishing by naked eyes, has high anti-interference capacity on common biological molecules, has quite efficient selectivity and can perform analysis through ultraviolet absorption and fluorescence spectrophotometry. The ESIPT type fluorescent probe is good in stability, can bestored and used for a long time, is applicable to growing environments of various living cells, can realize high-sensitivity detection of trace biological mercaptan in the cells, can be applied to cell and living imaging and has quite important application value.

The invention provides a derivatization separation and detection method of a glycyl-L-glutamine chiral isomer, and belongs to the field of analytical chemistry. The method comprises the steps that before the glycyl-L-glutamine chiral isomer enters a liquid chromatographic column, derivatization is conducted through a derivatization reagent, and the derivatization reagent adopts an N<alpha>-(5-fluorine-2,4-dinitrophenyl)-L-amino acid compound. The separation determination method is high efficiency liquid chromatography. The used chromatographic column is a chromatographic column which takes base octadecyl bonded silica as filler. The separation and detection method mainly comprises the steps of preparing a sample solution and a derivative solution, conducting a derivative reaction, conducting derivative reaction aftertreatment and conducting separation determination through a high performance liquid chromatographic instrument. The pre-column derivatization method has the advantages that the reaction condition is mild, derived products are stable, the reaction velocity is high, the side reactions are few, determination is not disturbed by the excess derivatization reagent, and separation between glycyl-L-glutamine and the chiral isomer of the glycyl-L-glutamine can be achieved by using an ordinary chromatographic column.

The invention belongs to the field of chemical analysis and detection, and particularly discloses a water-soluble double-photon fluorescent probe capable of being directly used for detecting hydrogensulfide in red wine, cancer cells and tissues, and a preparation method and application of the water-soluble two-photon fluorescent probe. According to the fluorescent probe, a coumarin derivative with a double-photon property is taken as a signal group, 2,4-dinitrophenyl ether is taken a hydrogen sulfide recognition group, and the structure of the fluorescent probe is represented as in a formula(I). The fluorescent probe is good in water solubility, high in selectivity, excellent in anti-interference capability and high in sensitivity, can be directly used for rapid detection of hydrogen sulfide in red wine, cancer cells and tissues, and has a good application prospect.

A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.

The invention discloses a molybdenum disulfide doped linear polymer modified graphene composite material and a preparation method thereof. First of all, 1,1'-bis(2,4-dinitrophenyl)-4,4'-bipyridinium dichloride BDB and oxidized graphene are prepared, then oxidized graphene modified by a linear bipyridine polymer is prepared through Suzuki reaction, then molybdate is exchanged to the linear bipyridine polymer through an anion-exchange process, then a moly-sulfide disulfide graphene composite material precursor is obtained through co-hydrothermal sulfidation with thioacetamide, and finally, the molybdenum disulfide graphene composite material is obtained through high-temperature desulphurization. According to the invention, the uniformly dispersed molybdenum disulfide graphene composite material is obtained based on charge interaction between the BDB and the molybdate, the preparatiton method is simple, the electrochemical perforamcne of the prepared material is excellent, at the same time, the method has the potential of large-scale preparation, and the method can be applied to such fields as electrocatalysis, hydrogen evolution catalysis, supercapacitors and the like.

A preparing method of N-(2,4-dinitrophenyl)rhodamine B-hydrazide and an application of the N-(2,4-dinitrophenyl)rhodamine B-hydrazide for detection of Cu(II) are disclosed. The preparing method includes a step of reacting rhodamine B acid chloride and 2,4-dinitrophenylhydrazine to generate the N-(2,4-dinitrophenyl)rhodamine B-hydrazide. The N-(2,4-dinitrophenyl)rhodamine B-hydrazide itself is free of color and fluorescence, but can generate coloring and fluorescence generating reactions selectively with Cu<2+>, so that a colorless substance is turned into a system with excellent optical properties, and detection sensitivity and selectivity for the Cu<2+> are largely enhanced. Accordingly, the N-(2,4-dinitrophenyl)rhodamine B-hydrazide is suitable for high-selectivity and high-sensitivity detection of the Cu<2+>. The detection can be performed by a light absorption spectrum method and a fluorescence spectrum method.

The invention discloses near-infrared fluorescent dye THX-Np capable of achieving mitochondrial localization and a preparation method and application of the near-infrared fluorescent dye THX-Np. The structural formula of the dye is shown in (I). The dye has a stable pH fluorescence opening effect in an aqueous phase under the conditions of different pH values, and 2,4-dinitrophenyl ether derivatives prepared by using the dye can be applied to detection of hydrogen sulfide. The invention has the following advantages that the fluorescent dye has pH stability, large Stokes shift, good water solubility, good cell membrane permeability and excellent mitochondrial localization performance, and hydroxyl groups and carboxyl groups of the dye can be easily modified so as to produce tracers relatedto organisms, the 2,4-dinitrophenyl ether derivatives prepared through etherification of hydroxyl groups of the dye have excellent selective recognition of hydrogen sulfide, and an excellent selectivefluorescent probe of hydrogen sulfide detection.

The invention discloses a preparation method of dapsone, and more in particular relates to a method for preparing dapsone by a one-pot method. The preparation method of dapsone is characterized by comprising the steps of: by taking nitrobenzenesulfonyl chloride as the raw material and methanol as the solvent, and carrying out a desulfurizing coupling reaction under the action of copper acetate to obtain para-dinitrophenyl sulfone, and then adding sodium hydrosulfite to the reaction system to obtain the dapsone. According to the invention, the methanol is taken as the solvent, and sodium hydrosulfite is added as the reducing agent to obtain the dapsone by the one-pot method under the action of the copper acetate without any ligand, the protection of an inert gases and separation; and the total yield can be 88%.

The general structural formula of the arylpyrazoline light-emitting compound substituted by the 5-position condensed aromatic hydrocarbon is as follows: wherein, R1 is 4-nitrophenyl, 4-cyanophenyl, 4-esterylphenyl, 2-nitro Phenyl, 2-cyanophenyl, 2-esterylphenyl, 2,4-dinitrophenyl or hydrogen atom, R2 is 4-nitro, 4-cyano, 4-esteryl, 2-nitro Group, 2-cyano group, 2-ester group or 2,4-dinitro group, R3 is 9-anthracenyl group, 9-phenanthrenyl group or 1-pyrenyl group. The preparation method is as follows: firstly, condensing aromatic aldehydes with condensed rings and aromatic ketones substituted with electron-withdrawing groups on the benzene ring to generate chalcone intermediates, and then condensing with aromatic hydrazines substituted with electron-withdrawing groups on the benzene ring to form a ring; The generated pyrazoline molecule has two chromophores, the "arylpyrazoline" and the 5-position anthracycline, which enhance charge transfer in the molecule, and the luminescence spectrum of the 5-position aromatic condensed ring is compatible with the arylpyrazoline chromophore The absorption spectra overlap to produce effective energy transfer, improve the luminescence and two-photon fluorescence of pyrazoline molecules.

The invention discloses a closing-opening molecular fluorescent probe of chalcone linked to a 2, 4-dinitrophenyl group. The closing-opening molecular fluorescent probe is used for detection of toxic thiophenol in the offshore waters. The closing-opening molecular fluorescent probe has no fluorescence. Thiophenol improves the fluorescence and strong yellow-green fluorescence is produced. Through use of thiophenol in the probe molecule probe-KCN1 solution, the fluorescence intensity is increased by 40 times. When the thiophenol has a concentration of 0-10 micromoles per liter, the fluorescence intensity of the probe-KCN1 detection system shows a good linear relationship with the concentration of thiophenol. The closing-opening molecular fluorescent probe can be used for detecting a thiophenol compound at a low concentration in the seawater sample and has a practical application value.

Belonging to the technical field of chemical industry, the invention relates to a soluble polymerized sulphur production process and equipment. The production process includes: putting sulphur powder into a mixed reaction kettle A, conducting stirring and increasing the rotation speed, putting aniline methyltriethoxysilane and gamma-(methylacryloyloxy)propyltrimethoxysilane into the reaction kettle A, and when the materials in the kettle are stirred evenly, discharging the premix for stand-by use; putting sulphur powder into a reaction kettle B, then adding the premix and 2-(2, 4-dinitrophenylthio)benzothiazole, 2-mercaptothiazoline, and 1, 1, 3-tri(2-methyl 4-hydroxy-5-tert-butylphenyl)butane, increasing the rotation speed and performing stirring for 4h; and cooling the materials in the reaction kettle. The production equipment mainly consists of a kettle body, a motor, double helix stirring blades, a spatula and scraping chains, materials are stirred by the double helix stirring blades, spatula and scraping chains. The product provided by the invention adopts a low temperature and atmospheric pressure production technology, the technology has the characteristics of safety, low energy consumption and high sulphur conversion rate, and the formula completely rejects flammable and explosive toxic solvents.

The invention discloses a fluorescence probe for quickly identifying thiophenol. The fluorescence probe reacts with the thiophenol to generate a reaction product with high fluorescence. The fluorescence probe can quickly react with the thiophenol thorugh a 2,4-dinitrophenyl ether base identification group with relatively high electron absorbing capacity and generate the reaction product with highfluorescence, and is high in sensitivity and high in response speed; the fluorescence probe is relatively high in interference resistance, can specifically identify the thiophenol and is applicable tothiophenol detection of various samples with relatively high interference strength.

For measuring carbonyl value, which is as one of index for determining whether oil and fat is oxidized or in spoilage, previous method uses toxic solvent benzene, and possesses disadvantages of great quantity of solvent, fussy operation, and long operation time so that it is not suitable to use in site. The invention discloses quick, accurate, and safety detection method applicable to working site. The invention uses safe normal butyl alcohol as organic solvent. Using reaction between carbonyl compound in oil and fat, and 2,4 - dinitrophenyl hydrazine generates 2,4 - dinitro hydrazo benzene. Under alkalinity condition, 2,4 - dinitro hydrazo benzene generates quinone in red color of grape wine. The said color at place of 380nm-420nm possesses extinction characteristic. Thus, using colorimetry can process quantitative analysis.

A chain porphyrin polymer is a chain-structure polymer formed by alternate chaining of diaminophenyl porphyrin and terephthalaldehyde through aldehyde ammonia condensation polymerization. The chain porphyrin polymer and a preparation method thereof belongs to the field of macromolecular syntheses. The chain porphyrin polymer is used for catalyzing and degrading organic pollutants in wastewater. Asynthetic method of the chain porphyrin polymer utilizing macromolecular condensation polymerization reaction, wherein tetraphenylporphyrin is nitrified through fuming nitric acid to obtain dinitrophenyl porphyrin, then the dinitrophenyl porphyrin is reduced to the diaminophenyl porphyrin, under protection of N2, the diaminophenyl porphyrin obtained from separating by column chromatography and terephthalaldehyde are catalyzed by glacial acetic acid and subjected to terephthalaldehyde condensation to form Schiff base (-C=N-), and the chain-structure polymer with phenyl porphyrin as a structureunit is generated. The synthesized chain porphyrin polymer is high in thermal stability, recyclable and promising in application prospect.

The invention discloses a preparation method of a 1, 8-naphthalimide hydrogen sulfide fluorescent molecular probe and application of the 1, 8-naphthalimide hydrogen sulfide fluorescent molecular probe in hydrogen sulfide recognition and detection. The fluorescent molecular probe with double recognition sites is obtained by using N-(p-benzyl)-4-hydroxy-1, 8-naphthalimide as a fluorescent matrix and 2, 4-dinitrophenyl ether as a recognition group, hydrogen sulfide performs thiolysis on the 2, 4-dinitrophenyl ether, so that hydroxyl is exposed out of a fluorophore, and the fluorescence intensity is enhanced (by more than 30 times). The fluorescent molecular probe has the advantages of stable photochemical properties, high fluorescence quantum yield, low price and the like, specifically recognizes hydrogen sulfide, is not interfered by ions such as Na<+>, Ca<2+>, NO2<->, H2O2, ClO<->, HSO4<->, CN2H4S, HSO3<->, S2O5<2-> and S2O8<2->, has a linear detection range increased to 0-40 [mu] mmol / L, can be used for quantitative detection of high-concentration hydrogen sulfide in river water, rainwater, drinking water, drilling fluid, marsh liquid and the like, and has potential practical application value.

The invention discloses a method for synthesizing 1H, 1'H-(2,2'-bis-benzimidazole)-5,5'-diamine. The method comprises the steps of obtaining N,N'-bis(2,4-dinitrophenyl)oxamide through condensation reaction between oxalyl chloride and 2,4-dinitraniline, obtaining N,N'-bis(2,4-diaminophenyl)oxamide through catalytic hydrogenation of the N,N'-bis(2,4-dinitrophenyl)oxamide and obtaining the 1H,1'H-(2,2'-bis-benzimidazole)-5,5'-diamine through dehydration cyclization of the N,N'-bis(2,4-diaminophenyl)oxamide. The method disclosed by the invention has a shorter synthesizing path, high technological safety, convenience to operate, lower production cost, higher reaction yield and especially smaller environmental pollution, so that the method is suitable for industrial production; in addition, raw materials of the method are cheap and easy to obtain.

The invention discloses structures of nitrophenyl-benzo[1,2,3] triazole, dinitrophenyl-benzo[1,2,3] triazole and a dinitrophenyl-benzo[1,2,3] triazole derivative, and mass spectrometry information thereof. In addition, the invention also discloses preparation methods for the nitrophenyl-benzo[1,2,3] triazole, the dinitrophenyl-benzo[1,2,3] triazole and the dinitrophenyl-benzo[1,2,3] triazole derivative. According to the preparation methods, novel compound structures of the nitrophenyl-benzo[1,2,3] triazole and the dinitrophenyl-benzo[1,2,3] triazole derivative are prepared, which can be applied to novel materials for the fields, such as energetic materials, oil exploitation and space exploration. Synthesis methods provided by the invention have the characteristics that raw materials are easy to obtain, the operation is simple and convenient, the safety is high, the process is controllable and the like.

This invention relates to compositions comprising haptenized tumor cells and extracts thereof, methods for preparing the compositions, vaccines comprising such haptenized tumor cells, and methods for treating cancer with such vaccines. In a specific embodiment, melanoma cells are haptenized with a dinitrophenyl group, and used for treatment of melanoma patients having metastatic disease. Preferably, patients are given a first vaccine dose containing haptenized cells to “prime” the immune system. Subsequently, patients are injected with an immunomodulatory compound such as cyclophosphamide. In a preferred embodiment, an appropriate time period after the “priming” vaccine dose, additional vaccine doses containing a mixture of haptenized cells and an adjuvant are administered. The described treatment plan is more effective for eliciting favorable anti-tumor immune responses.

An environmentally friendly methods for making 2,4-dinitrophenyl compound comprising, providing a nitromalondialdehyde, providing a nitroacetone, reacting the nitromalondialdehydes with the nitroacetone to produce a mixture, and subjecting the mixture to a cyclodehydrative mechanism to produce environmentally friendly 2,4-dinitrophenyl compound. Embodiments of the present invention include the 2,4-dinitrophenyl compound produced by the methods of described above.

The invention discloses a holarrhine alkaloid derivative and an application of holarrhine alkaloid derivative. The structure of the holarrhine alkaloid derivative disclosed by the invention is as general formula (I), wherein R represents substituted phenyl or substituted naphthyl and specifically represents the following groups: phenyl, paranitrophenyl, p-methyl phenyl, p-methoxy phenyl, 2,4-dichlorophenyl, p-chlorophenyl, 2,4-dinitrophenyl, 2-naphthyl, 1-naphthyl, 4-chlorine-1-naphthyl and methoxy phenyl. The invention further discloses an application of the holarrhine alkaloid derivative in preparation of a medicine for preventing and treating alzheimer's disease or a medicine for improving the intelligence.

The invention relates to a pre-column derivatization reagent for detection on taurine and a preparation process for the same, wherein the derivatization reagent has a chemical name of 2-chlor-3,5-dinitrophenyl formamide, moderate conditions of a reaction with the taurine, a fast speed, and high sensitivity; the problem mainly solved according to the invention is to find a derivatization reagent easy to be subjected to pre-column derivatization with the taurine, high in detection sensitivity, and easy to store; and the 2-chlor-3,5-dinitrophenyl formamide found and prepared according to the invention is stable in property, fast in the speed of reaction with an amino compound, and capable of being widely used as an amino acid compound, especially used as a high-sensitivity liquid chromatography derivatization reagent for detection on taurine.