362 results about "Sialic acid" patented technology

The invention relates to a multi-effect bionic liposome transdermal repair carrier and a preparation method and application thereof.The multi-effect bionic liposome transdermal repair carrier comprises active ingredients and a nano-carrier, and the active ingredients comprise sialic acid, saccharomycetes / rice fermentation product filtrate, arginine / lysine polypeptide and vitamin E in the mass ratio of (1-10): (2-8): (0.1-1): (0.5-4); the nano-carrier is prepared from the following raw materials: phospholipid, an emulsifier, polyhydric alcohol and water. Compared with the prior art, the anti-aging, whitening and moisturizing composition has the advantages of enhancing skin moisturizing and anti-oxidation effects, promoting collagen synthesis and inhibiting tyrosinase to achieve remarkable anti-aging, whitening and moisturizing effects and the like.

The invention relates to the technical field of synthetic biology, metabolic engineering and cell culture. The present invention provides a cell for the production of a negatively charged, preferably sialylated, oligosaccharide wherein the cell is genetically engineered to have or express, preferably overexpress, a hydrolytic UDP-N-acetyl-D-glucosamine-2-epimerase. The invention further provides the use of said cells in culture or incubation. Also described are methods of producing negatively charged, preferably sialylated oligosaccharides using the cells, and purification of the negatively charged, preferably sialylated oligosaccharides.

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

Compounds having Formulae (I)-(V), compositions comprising at least one compound selected from compounds having Formulae (I)-(V), and methods of using these compounds and compositions, including methods of treating Alzheimer's disease using these compounds and compositions, are disclosed. # imgabs0 #

The present invention provides a lipid nanoparticle comprising an entity that contains one or multiple sialic acid (SA) moieties, the entity is preferably a ganglioside, a ganglioside derivative, a ganglioside mimetic, or a combination thereof. The lipid nanoparticle of the present invention can effectively present SA moieties on the surface to bind Siglecs and complement factors to, inter alia, mitigate inflammation. The present invention also provides methods of using said lipid nanoparticle compositions described herein for pharmaceutical applications. For example, the lipid nanoparticles provided herein are useful for treating inflammatory and autoimmune diseases.

The invention discloses cubilose sialic acid glycan peptide capable of improving lung inflammation and application of the cubilose sialic acid glycan peptide. The ratio of fucose modified sugar chains in the cubilose sialic acid glycan peptide is 30-60%, the ratio of sialic acid modified sugar chains in the cubilose sialic acid glycan peptide is 20-40%, the core structure of the fucose type is (HexNAc) 3 (Man) 3 (Fuc) 1, the core structure of the sialic acid type is (HexNAc) 3 (Man) 3 (Gal) 1 (Fuc) 1 (NeuAc) 1, the neutral sugar dissolution rate of the cubilose sialic acid glycan peptide is high, and the glycopeptide shown in SEQ ID NO 2 has a good inflammation improving effect.

The present disclosure relates compositions comprising milk fat globule membrane (MFGM) and a mixture of HMOs with at least one fucosylated HMO and at least one HMO selected from neutral core HMOs or sialylated HMOs. The compositions have potential health benefits in terms of improving brain health, immunity and recovery time following exercise, by increasing the formation of glutamic acid.

The present invention discloses drug-loaded nanoparticles targeting neuraminidase, as well as a preparation method and application thereof, belonging to the technical field of pharmaceutical preparations. The present invention connects polysialic acid and polylactic acid glycolic acid via an ester bond to obtain drug-loaded nanoparticles with stable structure and performance. The drug-loaded nanoparticles have the function of targeting neuraminidase and have relatively high efficiency in entering cells. Furthermore, antibiotics are dispersed in the drug-loaded nanoparticles, and an inflammatory modulator is adsorbed on the surface of the drug-loaded nanoparticles. The drug-loaded nanoparticles can deliver the antibiotics and the inflammatory modulator to Grasseria suis in pigs, releasing the antibiotics and the inflammatory modulator in the slightly acidic environment of the infected site in the pig, thereby efficiently killing Grasseria suis and reducing inflammatory damage caused by bacterial infection and toxic damage of the antibiotics. Therefore, the present invention has good application prospects in the preparation of drugs for preventing and / or treating Grasseria suis infection.

The invention relates to a NeuC mutant, a host cell containing the NeuC mutant and a method for producing sialic acid by fermentation. The invention discloses a NeuC mutant, a coding nucleic acid thereof, a vector, a host cell containing the vector, and a method for producing sialic acid through fermentation by using the host cell. Compared with wild NeuC, the NeuC mutant can obviously improve the yield of sialic acid. The sialic acid yield can reach 61 g / L after high-density fermentation culture is performed for 60 h in a 3L fermentation tank.

The present invention relates to a fermented product containing a component X, a component Y, and a component Z, wherein the component X is sialic acid or a sialylated oligosaccharide, the content of the component X per solid content of the fermented product is 80 mass% or more, the component Y is acetic acid, the ratio (component Y content / component X content) of the content of the component Y to the content of the component X in the fermented product is 0.010 or less on a mass basis, the component Z is at least one of a component Z1 and a component Z2, the component Z is a component other than the component X, the component Z1 is a neutral sugar, and the component Z2 is an acidic sugar.

The invention discloses a sialic acid molecular imprinting extraction method, and belongs to the technical field of separation and purification. The invention discloses a sialic acid molecular imprinting extraction method which comprises the following steps: dissolving a sialic acid standard substance and a functional monomer in a binary pore-foaming agent-ionic liquid, adding a cross-linking agent and an initiator, polymerizing under ultraviolet light to prepare a high-molecular polymer, and washing off the sialic acid standard substance to prepare a molecular imprinting polymer; specifically adsorbing sialic acid in the fermentation liquor by using the molecularly imprinted polymer; performing graded elution on sialic acid by using a methanol-water mixed solution and a methanol-acetic acid mixed solution; and performing rotary evaporation concentration, anti-solvent crystallization, separation and drying on the bird's nest acid eluent. Tests show that the sialic acid purity is greater than or equal to 99%, and can meet the requirements of the sialic acid in the fields of food, health care, medicine, cosmetics and the like. According to the method, sialic acid can be efficiently separated and extracted from sialic acid fermentation liquor, the product yield is increased, the extraction procedures are reduced, and the production cost and the discharge amount of three wastes are reduced.

The present disclosure relates to a composition for balancing the immune system of a neonatal or infant subject, the composition comprising an effective amount of 2 '-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6 '-sialyllactose, and lactose-N-tetrasaccharide.

The invention discloses a nutritional composition containing phospholipids and sialic acid oligosaccharides and capable of promoting construction of a neural network. The invention provides a nutritional composition, the nutritional composition comprises the following necessary components: a phospholipid component and a sialic acid oligosaccharide component, and in the nutritional composition, the molar ratio of the phospholipid component to the sialic acid oligosaccharide component is (1-10): (0.01-5). The composition provided by the invention has the advantages that the components are synergistic, the construction of a neural network is effectively promoted, the application range is wide, and various foods or health-care products can be conveniently added or prepared.

The invention relates to the technical field of biological medicine, in particular to a cerium oxide nano-enzyme delivery system based on carried neutrophil, and the system comprises cerium oxide nano-particles; the surfaces of the cerium oxide nanoparticles are coated with the lipoic acid zwitterionic polymer layers; the sialic acid molecules and the protocatechuic aldehyde molecules are modified on the surface of the lipoic acid zwitterionic polymer layer; wherein the system realizes breakthrough of a blood brain barrier and precise delivery of ischemic lesions through mediation targeting delivery of L-selectin on the surface of neutrophile granulocyte, and based on a cerium oxide nano-enzyme delivery system carrying neutrophile granulocyte, a'antioxidation-anti-inflammation-angiogenesis promotion-neural restoration 'quadruple synergistic mechanism is integrated, so that the system has the advantages that the system is simple in structure and convenient to use, and the application prospect is wide. And a new strategy of multi-target intervention is provided for cerebral arterial thrombosis treatment.

The invention belongs to the field of synthetic biology and metabolic engineering, and particularly relates to high-yield sialyl acyl-N-tetrasaccharide a escherichia coli as well as a construction method and application thereof. According to the method, recombinant escherichia coli is taken as an initial strain, and a beta1, 3-galactosyl transferase gene wbgO from escherichia coli O55: H7 is integrated into a chromosome in a multi-copy form, so that a lactyl-N-tetrasaccharide efficient synthesis chassis strain without plasmid dependence is constructed. And overexpression neuC, neuB, neuA and Nm3ST are introduced through plasmids, so that the supply of glycosyl donors is improved. The expression intensity of uridine diphosphate-N-acetylglucosamine synthesis pathway genes glmM and glmUS is regulated through ribosome binding site engineering, and a cytidine triphosphate regeneration module is introduced, so that the yield of sialyloyl-N-tetrasaccharide a is increased. And finally, the maximum titers of the sialyloyl-N-tetrasaccharide a respectively reach 2.238 g / L and 8.178 g / L by shaking a flask and culturing in batches. The invention provides an efficient technical scheme for industrial production of complex breast milk oligosaccharide.

The invention belongs to the technical field of nutritional compositions, and particularly relates to a composition, a preparation method thereof and application of the composition in protecting brain health. The composition provided by the invention is prepared from docosahexaenoic acid, 3-sialic acid lactose and phosphatidylserine. The composition is clear in sleep improvement effect, can effectively solve the problem of sleep maintenance caused by sleep deprivation, has the effects of protecting neural functions, improving memory and relieving anxiety from multiple dimensions, and can repair brain tissue damage and protect brain health. Based on the composition, products for assisting in improving memory, preventing and treating insomnia and anxiety, regulating neurodevelopmental disorders, protecting brain health and improving concentration can be developed, the health requirements of different people are comprehensively met, and the application value is high.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention relates to the technical field of biomedical engineering and drug delivery, and particularly discloses a novel targeted drug delivery system based on rose fruit extracellular vesicles, which comprises the following steps: preparing rose fruit source extracellular vesicles; preparing a medicine carrying vesicle; preparing targeted modified drug-loaded vesicles; according to the application disclosed by the invention, a stem related pathway is specifically down-regulated through the components of the rose fruits, and meanwhile, conventional tumor cells are killed by using adriamycin, so that a complementary treatment mechanism is formed, and the tumor recurrence risk is fundamentally reduced. The phenylboronic acid group of DSPE-PEG-PBA is used for specifically recognizing sialic acid over-expressed on the surface of a tumor cell, so that the enrichment of the medicine in tumor tissues is remarkably improved, and meanwhile, the distribution of normal tissues is reduced. By means of the natural biocompatibility and low immunogenicity of the RHNVs and in combination with the long circulation characteristic of a PEG chain, the delivery efficiency is guaranteed, the systemic toxicity is reduced to the maximum extent, and particularly the cardiotoxicity and myelosuppression of adriamycin are relieved.

Compositions and related methods are provided, the compositions comprising an anti-TNF alpha antibody sialylated with one or more N-acetylneuraminic acid molecules. In some embodiments, methods for treating or preventing sarcoidosis and / or granulomegaly or formation in a patient in need thereof are provided.

The invention relates to the technical field of cosmetics, in particular to a method for improving the stability of a sialic acid-p-hydroxyacetophenone solution. The method comprises the following steps: mixing p-hydroxyacetophenone with a stabilizer, and then mixing with sialic acid to obtain sialic acid-p-hydroxyacetophenone mixed liquor; the stabilizer is sulfite and / or hydrosulfite, and the addition amount of the stabilizer is 0.005%-0.2% by mass. Aiming at the problems of discoloration of sialic acid-p-hydroxyacetophenone solution and loss of sialic acid, the invention provides a method, all the raw materials are mixed according to a specific sequence on the basis of a specific stabilizer through a specific processing technology, the stability of the obtained solution is remarkably improved, the solution does not discolorate after being placed for a long time, the loss rate of sialic acid is low, and the method is suitable for industrial production. The method has important application value in the field of production of cosmetics containing sialic acid at the same time.

The invention provides a nutritional composition capable of influencing protein expression of brain hippocampus and application of the nutritional composition. The nutritional composition of the present invention comprises sialic acid, sphingomyelin and phosphatidylcholine, in which the ratio of the sum of the contents of sphingomyelin and phosphatidylcholine to the content of sialic acid is 1: 1 by weight, and the ratio of sphingomyelin to phosphatidylcholine is 0.2: 1 to 1.4: 1 based on the total weight of sphingomyelin and phosphatidylcholine. The composition can increase the gray matter volume of the brain and can improve the expression of BDNFmRNA of the hippocampus and the prefrontal cortex.

The invention relates to the technical field of functional food, in particular to a sialic acid composition with whitening and skin state improving functions and a preparation method of the sialic acid composition. The sialic acid composition comprises sialic acid, poria cocos, Chinese yam, lily, radix polygonati officinalis, peach kernels, rosa rugosa plena and pyrroloquinoline quinone disodium salt. The composition comprises the following components in parts by weight: 8-18 parts of sialic acid, 2-12 parts of the sum of poria cocos and Chinese yam, 2-12 parts of the sum of lily and polygonatum odoratum, 2-12 parts of the sum of peach kernel and rosa rugosa plena, and 1-6 parts of pyrroloquinoline quinone disodium salt. Starting with the functions of soothing liver-qi stagnation, tonifying spleen and moistening lung, the sialic acid and the characteristics of the medicinal and edible medicinal materials are combined for compatibility, the effects of whitening and improving the skin state are synergistically exerted, and the sialic acid composition has important application value.

The present invention relates to an immunogenic polysaccharide-protein conjugate comprising a capsular polysaccharide (CP) from Streptococcus agalactiae (commonly known as group B streptococcus (GBS)) and a carrier protein, wherein the CP is selected from the group consisting of serotypes Ia, Ib, II, III, IV, V, VI, VII, VIII, and IX, and wherein the sialic acid level of the CP is higher than about 60%. The present invention also relates to methods of making the conjugate and immunogenic compositions comprising the conjugate. The present invention also relates to an immunogenic composition comprising a polysaccharide-protein conjugate, wherein the conjugate comprises CP from GBS serotype IV and at least one additional serotype. The present invention further relates to methods of using the compositions disclosed herein to induce an immune response against GBS in a subject and / or to reduce or prevent invasive GBS disease in a subject. The antibodies generated can be used for the treatment or prevention of GBS infection by passive immunotherapy.

The present invention relates to a microorganism which has an enhanced activity of a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion of 20-37 amino acid residues on the N-terminal side, and which has an improved productivity of 3'-sialyllactose compared to the parent strain.

This invention provides a method and kit for improving the signal from sialic acid labeled with the dye 1,2-diamino-4,5-methylenedioxybenzene (“DMB”). The method comprises labeling the sialic acid with DMB in an aqueous solution containing a glycine amino acid whose pH is adjusted to 1.5–3.2 by an acid such as phosphoric acid or hydrochloric acid, and a reducing agent.

The invention discloses a protein complex for protecting mitochondria from being damaged, a pharmaceutical composition and application. According to the application, the influence of nitrate on mitochondrial injury in in-vivo radiation-induced salivary gland injury and in-vitro human salivary gland cell injury is evaluated, the action mechanism in the mitochondrial injury is further deeply explored, and a result shows that sialic acid transporter interacts with FKBP8, so that the mitochondrial injury can be inhibited, and the mitochondrial injury can be inhibited. In turn, particles are formed and anchored on the outer mitochondrial membrane, thereby maintaining membrane integrity. Therefore, the invention provides a brand new therapeutic target for preventing radiation-induced salivary gland injury and other mitochondrial membrane related diseases.

The application relates to the technical field of sialic acid application, in particular to application of sialic acid in promoting iron absorption of iron supplement agents. It is found for the first time that sialic acid with a daily intake of 0.8-3.5 mg / kg and ferrous iron with a daily intake of 0.2-0.5 mg / kg can promote the absorption of ferrous iron by human bodies. More specifically, the application claims an iron supplement agent containing ferrous iron drugs and sialic acid. The iron supplement agent provided by the application promotes iron supplement by compounding sialic acid and ferrous iron drugs, thereby improving the bioavailability of iron, regulating immunity, promoting intestinal health and achieving various biological activities while supplementing iron.

The present application relates to the application of an acidic goat milk oligosaccharide composition in the preparation of an anti-allergy drug or food, the composition comprising 24 acidic sugar chains 24SMAOs, among which, according to the classification of sialic acid structure, 12 Neu5Ac modified sialylation sugar chains, 10 Neu5Gc modified sialylation sugar chains and 2 sialylation sugar chains modified by both Neu5Ac and Neu5Gc, according to the classification of sialic acid connection mode, 11 alpha2,3 connected sialic acid sugar chains, 10 alpha2,6 connected sialic acid sugar chains and 3 sialic acid sugar chains connected by both alpha2,3 and alpha2,6. The composition belongs to a natural anti-allergy substance, has safety and effectiveness, and realizes excellent anti-allergy performance.

The invention belongs to the technical field of biological medicines, and particularly relates to application of a nattokinase preparation in preparation of a medicine for preventing or treating sepsis. The nattokinase preparation comprises an electrostatic complex prepared from nattokinase and an anionic compound, the mass ratio of the nattokinase to the anionic compound ranges from 1: 1 to 1: 32, and the anionic compound is selected from one or two of polysialic acid or hyaluronic acid. Research results show that the nattokinase preparation not only can significantly improve the survival rate and weight of sepsis mice, but also can effectively stimulate the organism to generate potent and lasting immune memory response, and enhance the risk of the mice to resist secondary or more lipopolysaccharide attacks.