136 results about "Sialic acid" patented technology

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention relates to the technical field of biomedical engineering and drug delivery, and particularly discloses a novel targeted drug delivery system based on rose fruit extracellular vesicles, which comprises the following steps: preparing rose fruit source extracellular vesicles; preparing a medicine carrying vesicle; preparing targeted modified drug-loaded vesicles; according to the application disclosed by the invention, a stem related pathway is specifically down-regulated through the components of the rose fruits, and meanwhile, conventional tumor cells are killed by using adriamycin, so that a complementary treatment mechanism is formed, and the tumor recurrence risk is fundamentally reduced. The phenylboronic acid group of DSPE-PEG-PBA is used for specifically recognizing sialic acid over-expressed on the surface of a tumor cell, so that the enrichment of the medicine in tumor tissues is remarkably improved, and meanwhile, the distribution of normal tissues is reduced. By means of the natural biocompatibility and low immunogenicity of the RHNVs and in combination with the long circulation characteristic of a PEG chain, the delivery efficiency is guaranteed, the systemic toxicity is reduced to the maximum extent, and particularly the cardiotoxicity and myelosuppression of adriamycin are relieved.

The invention relates to the technical field of cosmetics, in particular to a method for improving the stability of a sialic acid-p-hydroxyacetophenone solution. The method comprises the following steps: mixing p-hydroxyacetophenone with a stabilizer, and then mixing with sialic acid to obtain sialic acid-p-hydroxyacetophenone mixed liquor; the stabilizer is sulfite and / or hydrosulfite, and the addition amount of the stabilizer is 0.005%-0.2% by mass. Aiming at the problems of discoloration of sialic acid-p-hydroxyacetophenone solution and loss of sialic acid, the invention provides a method, all the raw materials are mixed according to a specific sequence on the basis of a specific stabilizer through a specific processing technology, the stability of the obtained solution is remarkably improved, the solution does not discolorate after being placed for a long time, the loss rate of sialic acid is low, and the method is suitable for industrial production. The method has important application value in the field of production of cosmetics containing sialic acid at the same time.

The present invention relates to a microorganism which has an enhanced activity of a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion of 20-37 amino acid residues on the N-terminal side, and which has an improved productivity of 3'-sialyllactose compared to the parent strain.

This invention provides a method and kit for improving the signal from sialic acid labeled with the dye 1,2-diamino-4,5-methylenedioxybenzene (“DMB”). The method comprises labeling the sialic acid with DMB in an aqueous solution containing a glycine amino acid whose pH is adjusted to 1.5–3.2 by an acid such as phosphoric acid or hydrochloric acid, and a reducing agent.

The invention discloses a protein complex for protecting mitochondria from being damaged, a pharmaceutical composition and application. According to the application, the influence of nitrate on mitochondrial injury in in-vivo radiation-induced salivary gland injury and in-vitro human salivary gland cell injury is evaluated, the action mechanism in the mitochondrial injury is further deeply explored, and a result shows that sialic acid transporter interacts with FKBP8, so that the mitochondrial injury can be inhibited, and the mitochondrial injury can be inhibited. In turn, particles are formed and anchored on the outer mitochondrial membrane, thereby maintaining membrane integrity. Therefore, the invention provides a brand new therapeutic target for preventing radiation-induced salivary gland injury and other mitochondrial membrane related diseases.

The application relates to the technical field of sialic acid application, in particular to application of sialic acid in promoting iron absorption of iron supplement agents. It is found for the first time that sialic acid with a daily intake of 0.8-3.5 mg / kg and ferrous iron with a daily intake of 0.2-0.5 mg / kg can promote the absorption of ferrous iron by human bodies. More specifically, the application claims an iron supplement agent containing ferrous iron drugs and sialic acid. The iron supplement agent provided by the application promotes iron supplement by compounding sialic acid and ferrous iron drugs, thereby improving the bioavailability of iron, regulating immunity, promoting intestinal health and achieving various biological activities while supplementing iron.

A dPSA-binding agent conjugate and methods of use to treat cancer and kill cancer cells.

The invention relates to a ligand targeting neutrophil and neurons as well as a preparation method and application of the ligand. The ligand for targeting the neutrophil and the neuron is prepared from the following raw materials in parts by weight: 30 to 50 parts of DSPE-PEG-Tet1, 2 to 3 parts of ketal thiol with carboxyl groups at two ends and 30 to 50 parts of a neutrophil targeting ligand, the neutrophil targeting ligand is one or more of sialic acid, an anti-CD66b antibody, an anti-CD177 antibody, an anti-CD16b antibody, N-formylmethionine peptide (fMLF) and an analogue thereof, a CXCR1 / CXCR2 binding peptide, an integrin binding peptide, a selectin ligand and lectin. According to the invention, sequential targeting of neutrophile granulocytes-neurons can be realized, target cells of ischemic lesions can be accurately protected, and the improvement effect on the cerebral infarction area of MCAO rats can be obviously enhanced.

The application provides use of zanamivir in preparation of a medicine for treating or preventing preeclampsia, and a corresponding medicine and treatment method; zanamivir can effectively improve high blood pressure, proteinuria, creatinine, neuraminidase and sialic acid elevation symptoms of preeclampsia, increase NO level, and improve pregnancy outcome. Zanamivir and hydroxyprogesterone caproate in combination show better effects.

Bacterial strains are provided having at least one of a reduced size, a sialic acid coat, inducibly altered surface antigens, and expression of PD-L1 or CTLA-4 antagonists and / or tryptophanase. The bacteria may have improved serum half-life, increased penetration into tumors, increased tumor targeting and increased antitumor activity. The bacteria are useful for delivery of therapeutic agents that treat neoplastic diseases including solid tumors and lymphomas.

The present application relates to the technical field of biotechnology, and particularly discloses a composition, a preparation method thereof and application in improving brain health. The present application finds that when oat green extract and sialic acid are combined in a specific ratio, the composition can simultaneously have the effects of promoting brain development and improving cognitive impairment in the elderly, and further proposes a composition comprising: oat green extract and sialic acid; the mass ratio of the oat green extract and the sialic acid is (0.7-41.5):(1-14). The composition of the present application can simultaneously promote brain development and improve cognitive impairment in the elderly, and provides an effective new solution capable of acting throughout the entire life cycle for improving brain health.

The invention discloses a visual bifunctional subtype influenza virus molecularly imprinted sensor as well as a preparation method and application thereof. The sensor comprises a fluorescent signal core and a molecularly imprinted polymer layer coated outside the fluorescent signal core, the fluorescent signal core is SiO2 microspheres coated with CdTe quantum dots, the molecularly imprinted polymer layer is internally provided with an imprinting cavity capable of specifically recognizing the subtype influenza virus, the three-dimensional space shape of the imprinting cavity is complementary to that of the subtype influenza virus, and the molecularly imprinted polymer layer is coated with CdTe quantum dots. Sialic acid molecules are covalently modified on the inner surface. The sensor can be used for quantitative detection or visual semi-quantitative detection of subtype influenza viruses such as H7N9 and H5N1, and integrates double functions of rapid screening and accurate quantification. The sensor disclosed by the invention is good in preparation reproducibility, high in selectivity, strong in anti-interference capability and suitable for analysis of complex biological samples, and a novel user-friendly and efficient method is provided for on-site monitoring and early warning of influenza viruses.

The application discloses a kind of probe set and its combination 3D printing microfluidic chip to the method for analyzing specific glycoprotein sialic acid on exosome surface, belong to biochemical analysis technical field.The present application is based on 3D printing microfluidic chip combined with three-probe co-localization signal amplification strategy, nucleic acid signal is converted into fluorescent signal, by directly reading fluorescent signal intensity, realize the analysis of sialic acid signal on the glycoprotein on the surface of exosome, which provides a new method for overcoming the defects and limitations of exosome clinical application in the prior art, reduces the loss of sample, improves the sensitivity of reaction, and provides a feasible method for exploring the possible relationship between different exosomes and their parent cell specific glycoprotein sialic acid and disease development, and using sialic acid for tumor screening and disease course determination.

The application belongs to the technical field of biological medicine, and particularly relates to a galactosamine-doxetaxel conjugate as well as a preparation method and application thereof. First, doxetaxel and 3,3'-diseleno-dipropionic acid are used as raw materials, and after heating reaction, 3,3'-diseleno-dipropionic acid doxetaxel is obtained. Then, the obtained 3,3'-diseleno-dipropionic acid doxetaxel and galactosamine are used as raw materials, and after heating reaction, galactosamine-3,3'-diseleno-dipropionic acid doxetaxel conjugate is obtained through column chromatography separation and purification. The obtained conjugate can be specifically recognized by endogenous lectin receptors (such as asialoglycoprotein receptor ASGPR) which are highly expressed on the surface of hepatoma cells, so as to be taken up by hepatoma cells through a receptor-mediated pathway, and has application prospect in the direction of hepatoma targeted treatment.

The invention discloses sialylated-N-acetyl lactosamine and synthesis thereof, sialic acid (Neu5Ac) and N-acetyl lactosamine (LacNAc) or sialic acid (Neu5Ac), galactose (Gal), N-acetyl glucosamine (GlcNAc) and the like are used as raw materials, and the sialylated-N-acetyl lactosamine is synthesized through a one-step method. The method for catalytically synthesizing 6 '-SLN and 3'-SLN through a novel one-pot multi-enzyme catalytic system containing an NTP cyclic regeneration system is developed, the method is low in production cost and high in synthesis efficiency, and a foundation is laid for industrialization of sialic acid oligosaccharides such as 6 '-SLN and 3'-SLN.

The technical problem of the present application is to rapidly stabilize lactone in a sugar chain. A sample preparation method is a sample preparation method for preparing a sample containing a sugar chain, the preparation method including the steps of: performing a lactonization reaction that lactonizes at least a portion of sialic acid contained in the sugar chain; and performing an amidation reaction that amidates the lactone of the lactonized sialic acid by adding an amidation reaction solution to the sample, the amidation reaction solution containing at least one selected from the group consisting of ammonia, amines, and salts thereof that react with the lactonized sialic acid.

Provided are compositions comprising an anti-TNFα antibody sialylated with one or more N-acetylneuraminic acid molecules, and associated methods. In some embodiments, provided are methods for treating or preventing sarcoidosis and / or granuloma size or formation in patients in need thereof. In some embodiments, an outcome of the methods of the present technology is increased relative to the same method comprising use of a corticosteroid.

The invention provides a method for synthesizing a sialic acid oligosaccharide library with various sulfonated structures by a chemical enzyme method, and researches on the binding activity of sialic acid binding immunoglobulin-like lectin (Siglecs). Specifically, sialic acid and 9-azido-sialic acid are used as chemical-like protecting groups through an enzyme-catalyzed sialylation reaction, a specific position of oligosaccharide is protected, and then a sulfonate group is introduced at a preset site by utilizing high efficiency of a chemical reaction. Sialic acid or 9-azido-sialic acid, which is used as a protecting group, is removed by sialyl hydrolase, so that a sulfated oligosaccharide framework structure is obtained. And finally, carrying out enzymatic extension to obtain sulfonated sialic acid oligosaccharides with diversified structures. By means of the strategy, 66 kinds of sulfonated sialic acid oligosaccharides can be rapidly and efficiently prepared, the oligosaccharide chip prepared from the sulfonated sialic acid oligosaccharides is used for conducting systematic high-throughput activity screening on 12 proteins in the Siglecc family, and the high-affinity glycosyl ligands of the Siglecc proteins are found through research.

The present invention relates generally to recombinant sialidases, methods and compositions for extending the serum half-life of the recombinant sialidases, and their use in the treatment of sialic acid-related disorders.

Provided in the present application are a glycosylated fusion protein, a nucleic acid molecule, an expression vector, a host cell and the use thereof. A first aspect of the present application provides the glycosylated fusion protein, comprising a murine Fc variant and a hepatitis B virus antigen, wherein the murine Fc variant is obtained by performing amino acid mutation and non-mammalian glycosylation modification on a murine Fc fragment, the murine Fc variant comprises at least one of alanine at position 223, alanine at position 228, alanine at position 230, leucine at position 330, and glutamic acid at position 332, the glycosylation modification does not comprise sialic acid modification, and the positions are numbered according to the EU numbering system. The fusion protein can bind to DC cells and activate DC cells, and thus promote the proliferation and activation of specific T cells.

Pharmaceutical compositions comprising anti-interleukin 12 (IL-12) interleukin 23 (IL-23) antibodies are described. The pharmaceutical compositions may comprise molecular attributes, such as oxidation of light chain W32, isoaspartic acid at heavy chain position D55; high molecular weight species such as dimers and / or oligomers, high mannose, sialylation, or acidic peak species. Method comprising administering the pharmaceutical compositions are described. Methods of manufacturing the pharmaceutical compositions are described.

The present application relates to the use of polysialic acid or its derivatives in anti-foreign body reaction, in particular, to a polysialic acid anti-foreign body reaction material for resisting biological contamination, for regulating macrophage phenotype to reduce inflammatory response, and for resisting foreign body reaction. The polysialic acid material includes a bulk material prepared from polysialic acid and its derivatives, or a coating material for surface modification of the material, which can resist protein adsorption, cell adhesion and adhesion of various microorganisms, can act on immune cells, such as regulating the phenotype of macrophages, thereby reducing acute and chronic inflammatory response, and has excellent anti-foreign body reaction effect as an implant material.

This invention relates to a sialylated glycoprotein detection reagent, its preparation method, and its application, belonging to the field of biodetection technology. The detection reagent uses purified water as a solvent and comprises the following components: surface-modified titanium dioxide nanomaterials, metal ions, a pH adjuster, and a solvent. The surface-modified titanium dioxide nanomaterials are obtained by dispersing titanium dioxide nanoparticles in an organic solvent and reacting them with a silane coupling agent. The pH value of the sialylated glycoprotein detection reagent is ≥ 8.0. The reagent of this invention utilizes the high affinity of modified titanium dioxide nanomaterials for sialic acid to specifically aggregate sialylated glycoproteins in blood into particles, facilitating observation. This reagent exhibits good stability, high sensitivity, and strong specificity, making it suitable for rapid tumor screening of blood smear samples.

The invention relates to the technical field of functional foods, and particularly discloses a dietary therapy composition for promoting repair of cranial nerve damaged cells and a preparation method. The composition is prepared from the following raw materials in parts by weight: artichoke peptide, taurine, phosphatidylserine, natural nicotinic acid, guarana extract, collagen peptide, acerola cherry fruit powder, inulin, yak bone marrow powder, vitamin B family, sialic acid and sucralose. The preparation method comprises the following steps: S1, pretreating the raw materials; s2, performing nano crushing; s3, low-temperature drying; s4, embedding the inulin; and S5, mixing and forming. The artichoke peptide and the phosphatidylserine are synergistically matched, and a compound component with liver and brain axis functions is taken into consideration, so that a multi-target synergistic effect is achieved, the requirements of cell repair and neural development are synchronously met, and compared with single-component supplement or neglect of the influence of liver detoxification on brain repair in the prior art, the brain repair effect is greatly improved. The problems of low repairing efficiency and insufficient component utilization rate are solved.

This invention relates to the fields of pharmaceuticals and nanomedicine, specifically disclosing the preparation of a dual-targeting liposome drug delivery system and its application in stroke. The dual-targeting liposome drug delivery system comprises neutrophils, artemisinin, edaravone, and liposomes. The liposomes co-load artemisinin and edaravone, and their surfaces are modified with sialic acid derivatives and phosphatidylserine to form liposome nanomedicines. The neutrophils act as carriers, transporting the drug across the brain border (BBB) ​​and targeting it to the ischemic brain region. This dual-targeting strategy achieves precise two-stage drug delivery to the ischemic brain region. This delivery system can inhibit NLRP3 inflammasome activation, reduce pyroptosis, and promote microglial polarization towards the M2 anti-inflammatory phenotype, thereby effectively treating cerebral ischemia-reperfusion injury or ischemic stroke.

A sugar chain mass spectrometry data analysis device, method, and medium. It is determined whether a sialic acid-containing sugar chain is derivatized as intended. The sugar chain mass spectrometry data analysis device includes a sugar chain information acquisition unit that acquires, based on a sugar chain composition, a plurality of sugar chains having a core structure composed of a plurality of monosaccharide residues of a plurality of types and mass information corresponding thereto; a mass variation factor setting unit that sets a mass variation factor that causes a mass variation of the sugar chain; and a sugar chain theoretical mass calculation unit that calculates, based on the mass variation factor, a sugar chain theoretical mass-to-charge ratio of each sugar chain after the mass variation has occurred. The mass variation factor setting unit sets an ion species or salt that can be generated on a carboxyl group of a sialic acid of a sialic acid-containing sugar chain included in an analysis target and a sialic acid linking method that can cause a mass variation corresponding to a derivatization process. The sugar chain theoretical mass calculation unit estimates a mass variation amount according to a combination of the ion species or salt and the sialic acid linking method.

The invention provides a preparation method of a sialic acid extracting solution and a cigarette filter stick containing sialic acid obtained by the preparation method. The preparation method of the sialic acid extracting solution comprises the following steps: soaking cubilose raw materials with water to form cubilose feed liquid; the cubilose feed liquid is subjected to heat extraction, the heat extraction temperature ranges from 80 DEG C to 90 DEG C, and the heat preservation time ranges from 50 min to 70 min; and carrying out solid-liquid separation after the thermal extraction is finished to obtain a sialic acid extracting solution. According to the preparation method of the sialic acid extracting solution, a relatively high sialic acid extraction rate can be obtained, the dissolution of impurities is effectively inhibited, and the purity of the sialic acid extracting solution is favorably improved. The cigarette filter stick containing the sialic acid can more effectively adsorb and intercept harmful substances in smoke, meanwhile, the irritation of the smoke can be reduced, the taste of the smoke is milder, and the effects of reducing harm of the smoke and improving the sensory quality are considered.