250 results about "Sialic acid" patented technology

The invention relates to a multi-effect bionic liposome transdermal repair carrier and a preparation method and application thereof.The multi-effect bionic liposome transdermal repair carrier comprises active ingredients and a nano-carrier, and the active ingredients comprise sialic acid, saccharomycetes / rice fermentation product filtrate, arginine / lysine polypeptide and vitamin E in the mass ratio of (1-10): (2-8): (0.1-1): (0.5-4); the nano-carrier is prepared from the following raw materials: phospholipid, an emulsifier, polyhydric alcohol and water. Compared with the prior art, the anti-aging, whitening and moisturizing composition has the advantages of enhancing skin moisturizing and anti-oxidation effects, promoting collagen synthesis and inhibiting tyrosinase to achieve remarkable anti-aging, whitening and moisturizing effects and the like.

The invention relates to the technical field of synthetic biology, metabolic engineering and cell culture. The present invention provides a cell for the production of a negatively charged, preferably sialylated, oligosaccharide wherein the cell is genetically engineered to have or express, preferably overexpress, a hydrolytic UDP-N-acetyl-D-glucosamine-2-epimerase. The invention further provides the use of said cells in culture or incubation. Also described are methods of producing negatively charged, preferably sialylated oligosaccharides using the cells, and purification of the negatively charged, preferably sialylated oligosaccharides.

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The present disclosure relates compositions comprising milk fat globule membrane (MFGM) and a mixture of HMOs with at least one fucosylated HMO and at least one HMO selected from neutral core HMOs or sialylated HMOs. The compositions have potential health benefits in terms of improving brain health, immunity and recovery time following exercise, by increasing the formation of glutamic acid.

The present disclosure relates to a composition for balancing the immune system of a neonatal or infant subject, the composition comprising an effective amount of 2 '-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6 '-sialyllactose, and lactose-N-tetrasaccharide.

The invention discloses a nutritional composition containing phospholipids and sialic acid oligosaccharides and capable of promoting construction of a neural network. The invention provides a nutritional composition, the nutritional composition comprises the following necessary components: a phospholipid component and a sialic acid oligosaccharide component, and in the nutritional composition, the molar ratio of the phospholipid component to the sialic acid oligosaccharide component is (1-10): (0.01-5). The composition provided by the invention has the advantages that the components are synergistic, the construction of a neural network is effectively promoted, the application range is wide, and various foods or health-care products can be conveniently added or prepared.

The invention belongs to the technical field of nutritional compositions, and particularly relates to a composition, a preparation method thereof and application of the composition in protecting brain health. The composition provided by the invention is prepared from docosahexaenoic acid, 3-sialic acid lactose and phosphatidylserine. The composition is clear in sleep improvement effect, can effectively solve the problem of sleep maintenance caused by sleep deprivation, has the effects of protecting neural functions, improving memory and relieving anxiety from multiple dimensions, and can repair brain tissue damage and protect brain health. Based on the composition, products for assisting in improving memory, preventing and treating insomnia and anxiety, regulating neurodevelopmental disorders, protecting brain health and improving concentration can be developed, the health requirements of different people are comprehensively met, and the application value is high.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention relates to the technical field of biomedical engineering and drug delivery, and particularly discloses a novel targeted drug delivery system based on rose fruit extracellular vesicles, which comprises the following steps: preparing rose fruit source extracellular vesicles; preparing a medicine carrying vesicle; preparing targeted modified drug-loaded vesicles; according to the application disclosed by the invention, a stem related pathway is specifically down-regulated through the components of the rose fruits, and meanwhile, conventional tumor cells are killed by using adriamycin, so that a complementary treatment mechanism is formed, and the tumor recurrence risk is fundamentally reduced. The phenylboronic acid group of DSPE-PEG-PBA is used for specifically recognizing sialic acid over-expressed on the surface of a tumor cell, so that the enrichment of the medicine in tumor tissues is remarkably improved, and meanwhile, the distribution of normal tissues is reduced. By means of the natural biocompatibility and low immunogenicity of the RHNVs and in combination with the long circulation characteristic of a PEG chain, the delivery efficiency is guaranteed, the systemic toxicity is reduced to the maximum extent, and particularly the cardiotoxicity and myelosuppression of adriamycin are relieved.

The invention relates to the technical field of cosmetics, in particular to a method for improving the stability of a sialic acid-p-hydroxyacetophenone solution. The method comprises the following steps: mixing p-hydroxyacetophenone with a stabilizer, and then mixing with sialic acid to obtain sialic acid-p-hydroxyacetophenone mixed liquor; the stabilizer is sulfite and / or hydrosulfite, and the addition amount of the stabilizer is 0.005%-0.2% by mass. Aiming at the problems of discoloration of sialic acid-p-hydroxyacetophenone solution and loss of sialic acid, the invention provides a method, all the raw materials are mixed according to a specific sequence on the basis of a specific stabilizer through a specific processing technology, the stability of the obtained solution is remarkably improved, the solution does not discolorate after being placed for a long time, the loss rate of sialic acid is low, and the method is suitable for industrial production. The method has important application value in the field of production of cosmetics containing sialic acid at the same time.

The present invention relates to a microorganism which has an enhanced activity of a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion of 20-37 amino acid residues on the N-terminal side, and which has an improved productivity of 3'-sialyllactose compared to the parent strain.

This invention provides a method and kit for improving the signal from sialic acid labeled with the dye 1,2-diamino-4,5-methylenedioxybenzene (“DMB”). The method comprises labeling the sialic acid with DMB in an aqueous solution containing a glycine amino acid whose pH is adjusted to 1.5–3.2 by an acid such as phosphoric acid or hydrochloric acid, and a reducing agent.

The invention discloses a protein complex for protecting mitochondria from being damaged, a pharmaceutical composition and application. According to the application, the influence of nitrate on mitochondrial injury in in-vivo radiation-induced salivary gland injury and in-vitro human salivary gland cell injury is evaluated, the action mechanism in the mitochondrial injury is further deeply explored, and a result shows that sialic acid transporter interacts with FKBP8, so that the mitochondrial injury can be inhibited, and the mitochondrial injury can be inhibited. In turn, particles are formed and anchored on the outer mitochondrial membrane, thereby maintaining membrane integrity. Therefore, the invention provides a brand new therapeutic target for preventing radiation-induced salivary gland injury and other mitochondrial membrane related diseases.

The application relates to the technical field of sialic acid application, in particular to application of sialic acid in promoting iron absorption of iron supplement agents. It is found for the first time that sialic acid with a daily intake of 0.8-3.5 mg / kg and ferrous iron with a daily intake of 0.2-0.5 mg / kg can promote the absorption of ferrous iron by human bodies. More specifically, the application claims an iron supplement agent containing ferrous iron drugs and sialic acid. The iron supplement agent provided by the application promotes iron supplement by compounding sialic acid and ferrous iron drugs, thereby improving the bioavailability of iron, regulating immunity, promoting intestinal health and achieving various biological activities while supplementing iron.

The present application relates to the application of an acidic goat milk oligosaccharide composition in the preparation of an anti-allergy drug or food, the composition comprising 24 acidic sugar chains 24SMAOs, among which, according to the classification of sialic acid structure, 12 Neu5Ac modified sialylation sugar chains, 10 Neu5Gc modified sialylation sugar chains and 2 sialylation sugar chains modified by both Neu5Ac and Neu5Gc, according to the classification of sialic acid connection mode, 11 alpha2,3 connected sialic acid sugar chains, 10 alpha2,6 connected sialic acid sugar chains and 3 sialic acid sugar chains connected by both alpha2,3 and alpha2,6. The composition belongs to a natural anti-allergy substance, has safety and effectiveness, and realizes excellent anti-allergy performance.

The invention belongs to the technical field of biological medicines, and particularly relates to application of a nattokinase preparation in preparation of a medicine for preventing or treating sepsis. The nattokinase preparation comprises an electrostatic complex prepared from nattokinase and an anionic compound, the mass ratio of the nattokinase to the anionic compound ranges from 1: 1 to 1: 32, and the anionic compound is selected from one or two of polysialic acid or hyaluronic acid. Research results show that the nattokinase preparation not only can significantly improve the survival rate and weight of sepsis mice, but also can effectively stimulate the organism to generate potent and lasting immune memory response, and enhance the risk of the mice to resist secondary or more lipopolysaccharide attacks.

The invention discloses a nano vesicle with tissue targeting and mitochondrial localization as well as preparation and application of the nano vesicle. The nano-vesicle is a nucleus pulposus cell membrane entrapped with a small molecule mitochondrial membrane stabilizer, and tetra-acetylated N-acetamido mannose is added in the culture process of nucleus pulposus cells, so that a sialic acid derivative with an azide group is expressed on the surface of the nucleus pulposus cell membrane. Therefore, triphenylphosphine cations are modified on the surface of the nucleus pulposus cell membrane through a biological orthogonal reaction. The nano-vesicle can stabilize a mitochondrial membrane structure, improve cell aging-related secretion phenotype release and mitochondrial functions, block mtDNA release and SASP factor generation, realize anti-aging, anti-inflammatory and tissue degeneration intervention, can be used for preparing intervention drugs for tissue degeneration diseases related to mitochondrial membrane damage, and has broad application prospects. Or a drug for regulating mtDNA leakage and SASP release in a cell aging model. Wide application prospects and important clinical transformation values are realized.

A dPSA-binding agent conjugate and methods of use to treat cancer and kill cancer cells.

The invention relates to a ligand targeting neutrophil and neurons as well as a preparation method and application of the ligand. The ligand for targeting the neutrophil and the neuron is prepared from the following raw materials in parts by weight: 30 to 50 parts of DSPE-PEG-Tet1, 2 to 3 parts of ketal thiol with carboxyl groups at two ends and 30 to 50 parts of a neutrophil targeting ligand, the neutrophil targeting ligand is one or more of sialic acid, an anti-CD66b antibody, an anti-CD177 antibody, an anti-CD16b antibody, N-formylmethionine peptide (fMLF) and an analogue thereof, a CXCR1 / CXCR2 binding peptide, an integrin binding peptide, a selectin ligand and lectin. According to the invention, sequential targeting of neutrophile granulocytes-neurons can be realized, target cells of ischemic lesions can be accurately protected, and the improvement effect on the cerebral infarction area of MCAO rats can be obviously enhanced.

The application provides use of zanamivir in preparation of a medicine for treating or preventing preeclampsia, and a corresponding medicine and treatment method; zanamivir can effectively improve high blood pressure, proteinuria, creatinine, neuraminidase and sialic acid elevation symptoms of preeclampsia, increase NO level, and improve pregnancy outcome. Zanamivir and hydroxyprogesterone caproate in combination show better effects.

Bacterial strains are provided having at least one of a reduced size, a sialic acid coat, inducibly altered surface antigens, and expression of PD-L1 or CTLA-4 antagonists and / or tryptophanase. The bacteria may have improved serum half-life, increased penetration into tumors, increased tumor targeting and increased antitumor activity. The bacteria are useful for delivery of therapeutic agents that treat neoplastic diseases including solid tumors and lymphomas.

Disclosed herein is a polysialic acid (PSA)-polymer conjugated compound represented by Structural Formula (I): or a pharmaceutically acceptable salt thereof, wherein P is a poly (lactide-co-glycolide)-poly (ethylene glycol) copolymer (PLGA-PEG) and p is an integer from 4 to 200; nanoparticles comprising the same; and methods of treating ophthalmic diseases using the same. (I)

The invention belongs to the technical field of biomolecule detection, and particularly relates to a quantitative analysis method for sialic acid. According to the method, a basic type sialic acid standard substance is used as an internal standard substance, dimethylamine and deuterated dimethylamine are used as labeling reagents, amido bonds are generated between the labeling reagents and carboxyl groups in the internal standard substance and a biological sample, sialic acid is labeled at the same time, the samples with light and heavy labels are mixed, and the mixture passes through a reduction end of 3-nitrophenylhydrazine derivative sialic acid. After detection is completed through a liquid chromatography-mass spectrometry method, absolute quantitative analysis can be performed on sialic acid in a biological sample on the basis of combined use of an internal standard method and an external standard method. As O-acetylated sialic acid and non-O-acetylated sialic acid of the same type can generate similar secondary mass spectrums and same quantitative ions, absolute quantitative analysis can be approximately performed on O-acetylated sialic acid by using the method and the device.

Provided herein are methods of increasing sialic acid levels in recombinant glycosylated proteins, recombinant glycosylated proteins produced by these methods, and methods of treating subjects in need thereof using these recombinant glycosylated proteins.

The invention discloses a method for detecting sialic acid in a dairy product. The method comprises the following steps: pretreating raw materials: weighing a uniformly mixed liquid dairy product, adding water for diluting, adding a precipitant consisting of potassium ferrocyanide and zinc acetate, uniformly shaking, adding water for fixing the volume, uniformly shaking, and filtering with a filter membrane of 0.5 mu m or less to obtain a filtrate; the concentration of the liquid dairy product is 1-10g / 100mL, the concentration of the potassium ferrocyanide is 0.1-0.26 g / 100mL, and the concentration of the zinc acetate is 0.2-0.55 g / 100mL after the water is added to a constant volume; a chromatographic column is a hydrogen type cation chromatographic column, a 0.005-0.01 mol / L sulfuric acid aqueous solution is used as an eluent for isocratic elution, and after elution, a PAD detector is adopted for detection under the detection wavelength of 210-220 nm. The method is suitable for detecting sialic acid in milk, and has the advantages of simple sample treatment, low treatment cost and accurate detection.

The present application relates to the technical field of biotechnology, and particularly discloses a composition, a preparation method thereof and application in improving brain health. The present application finds that when oat green extract and sialic acid are combined in a specific ratio, the composition can simultaneously have the effects of promoting brain development and improving cognitive impairment in the elderly, and further proposes a composition comprising: oat green extract and sialic acid; the mass ratio of the oat green extract and the sialic acid is (0.7-41.5):(1-14). The composition of the present application can simultaneously promote brain development and improve cognitive impairment in the elderly, and provides an effective new solution capable of acting throughout the entire life cycle for improving brain health.

The invention discloses a visual bifunctional subtype influenza virus molecularly imprinted sensor as well as a preparation method and application thereof. The sensor comprises a fluorescent signal core and a molecularly imprinted polymer layer coated outside the fluorescent signal core, the fluorescent signal core is SiO2 microspheres coated with CdTe quantum dots, the molecularly imprinted polymer layer is internally provided with an imprinting cavity capable of specifically recognizing the subtype influenza virus, the three-dimensional space shape of the imprinting cavity is complementary to that of the subtype influenza virus, and the molecularly imprinted polymer layer is coated with CdTe quantum dots. Sialic acid molecules are covalently modified on the inner surface. The sensor can be used for quantitative detection or visual semi-quantitative detection of subtype influenza viruses such as H7N9 and H5N1, and integrates double functions of rapid screening and accurate quantification. The sensor disclosed by the invention is good in preparation reproducibility, high in selectivity, strong in anti-interference capability and suitable for analysis of complex biological samples, and a novel user-friendly and efficient method is provided for on-site monitoring and early warning of influenza viruses.

The application discloses a kind of probe set and its combination 3D printing microfluidic chip to the method for analyzing specific glycoprotein sialic acid on exosome surface, belong to biochemical analysis technical field.The present application is based on 3D printing microfluidic chip combined with three-probe co-localization signal amplification strategy, nucleic acid signal is converted into fluorescent signal, by directly reading fluorescent signal intensity, realize the analysis of sialic acid signal on the glycoprotein on the surface of exosome, which provides a new method for overcoming the defects and limitations of exosome clinical application in the prior art, reduces the loss of sample, improves the sensitivity of reaction, and provides a feasible method for exploring the possible relationship between different exosomes and their parent cell specific glycoprotein sialic acid and disease development, and using sialic acid for tumor screening and disease course determination.

The application belongs to the technical field of biological medicine, and particularly relates to a galactosamine-doxetaxel conjugate as well as a preparation method and application thereof. First, doxetaxel and 3,3'-diseleno-dipropionic acid are used as raw materials, and after heating reaction, 3,3'-diseleno-dipropionic acid doxetaxel is obtained. Then, the obtained 3,3'-diseleno-dipropionic acid doxetaxel and galactosamine are used as raw materials, and after heating reaction, galactosamine-3,3'-diseleno-dipropionic acid doxetaxel conjugate is obtained through column chromatography separation and purification. The obtained conjugate can be specifically recognized by endogenous lectin receptors (such as asialoglycoprotein receptor ASGPR) which are highly expressed on the surface of hepatoma cells, so as to be taken up by hepatoma cells through a receptor-mediated pathway, and has application prospect in the direction of hepatoma targeted treatment.