455 results about "Sialic acid" patented technology

The invention relates to a multi-effect bionic liposome transdermal repair carrier and a preparation method and application thereof.The multi-effect bionic liposome transdermal repair carrier comprises active ingredients and a nano-carrier, and the active ingredients comprise sialic acid, saccharomycetes / rice fermentation product filtrate, arginine / lysine polypeptide and vitamin E in the mass ratio of (1-10): (2-8): (0.1-1): (0.5-4); the nano-carrier is prepared from the following raw materials: phospholipid, an emulsifier, polyhydric alcohol and water. Compared with the prior art, the anti-aging, whitening and moisturizing composition has the advantages of enhancing skin moisturizing and anti-oxidation effects, promoting collagen synthesis and inhibiting tyrosinase to achieve remarkable anti-aging, whitening and moisturizing effects and the like.

The invention relates to the technical field of food processing, in particular to cubilose nourishing, conditioning and blood sugar reducing health-care food and a preparation process thereof. The cubilose enters the palace from the clear generation, becomes a defending tonic, is a medicinal and edible tonic, and has the beauty of a rare famous drug and an oriental treasure. The cubilose is rich in sialic acid, protein, amino acid and other bioactive substances, so that the cubilose has a nourishing effect and has certain health-care and conditioning effects on the lung, the kidney and the stomach. According to the preparation method, the cherry and the honeysuckle are subjected to synergistic fermentation through the rhamnolactobacilli and the saccharomycetes, so that the performance is improved, and the prepared cubilose health-care food has the effect of reducing blood sugar.

The invention relates to the technical field of synthetic biology, metabolic engineering and cell culture. The present invention provides a cell for the production of a negatively charged, preferably sialylated, oligosaccharide wherein the cell is genetically engineered to have or express, preferably overexpress, a hydrolytic UDP-N-acetyl-D-glucosamine-2-epimerase. The invention further provides the use of said cells in culture or incubation. Also described are methods of producing negatively charged, preferably sialylated oligosaccharides using the cells, and purification of the negatively charged, preferably sialylated oligosaccharides.

The invention belongs to the technical field of food, and relates to application of breast milk oligosaccharide in regulating brain immunity. Specifically, the invention relates to the non-therapeutic purpose of adjusting brain immunity by adjusting polarization of microglial cells M1 / M2, and the breast milk oligosaccharide is any one of 3-fucosyllactose, lactose-N-tetraose, lactose-N-neotetraose, 3 '-sialic acid lactose and 6'-sialic acid lactose. Research results show that a specific variety of breast milk oligosaccharides (especially 3-FL) can obviously inhibit the generation of neurotoxic factors NO, inflammatory factors TNF-alpha and IL-1beta in microglial cells, and promote the generation of anti-inflammatory cytokines IL-10 in the microglial cells, so that the state of the microglial cells is improved, damage repair is promoted, and the microglial cells are prevented from being damaged. Therefore, the effect of regulating brain immunity is achieved.

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The invention provides a preparation process of micromolecular cubilose peptide and a compound tablet thereof, and belongs to the technical field of protein and peptide. The preparation process of the micromolecular cubilose peptide comprises the following steps: freezing and crushing cubilose; and carrying out compound enzyme enzymolysis. The preparation method comprises the following steps: repeatedly freezing and crushing cubilose to prepare cubilose powder, adding the cubilose powder into deionized water, heating and stewing, and adding a compound enzyme formed by mixing neutral protease and flavourzyme for ultrasonic-assisted enzymolysis to obtain the micromolecular cubilose peptide, when the neutral protease and the flavourzyme are mixed according to the mass ratio of 1: (2.8-3.4) to conduct enzymolysis on the cubilose, hydrolysis can be conducted from multiple positions, smaller peptide fragments are formed, the hydrolysis degree is effectively improved, sialic acid originally combined with protein is also released along with hydrolysis of the protein, and therefore the content of sialic acid in the cubilose is increased. Therefore, the content of sialic acid in the prepared micromolecular cubilose peptide can be effectively improved.

Compounds having Formulae (I)-(V), compositions comprising at least one compound selected from compounds having Formulae (I)-(V), and methods of using these compounds and compositions, including methods of treating Alzheimer's disease using these compounds and compositions, are disclosed. # imgabs0 #

The present invention provides an anti-non-enzymatic glycosylation composition and its application, and a non-enzymatic glycosylation inhibitor. The anti-non-enzymatic glycosylation composition is composed of the following raw materials in parts by weight: 30 to 90 parts of sialic acid, 10 to 40 parts of ginkgo leaf extract, 10 to 40 parts of acerola extract, and 0.1 to 10 parts of rosemary extract. The composition of the present invention can achieve an anti-non-enzymatic glycosylation level comparable to that of the prior art by compounding sialic acid with ginkgo leaf extract, acerola extract, and rosemary extract in a specific ratio, while the stability is also improved. Applying it to health foods or cosmetics with anti-aging effects saves costs and is conducive to production and promotion.

The present invention provides a lipid nanoparticle comprising an entity that contains one or multiple sialic acid (SA) moieties, the entity is preferably a ganglioside, a ganglioside derivative, a ganglioside mimetic, or a combination thereof. The lipid nanoparticle of the present invention can effectively present SA moieties on the surface to bind Siglecs and complement factors to, inter alia, mitigate inflammation. The present invention also provides methods of using said lipid nanoparticle compositions described herein for pharmaceutical applications. For example, the lipid nanoparticles provided herein are useful for treating inflammatory and autoimmune diseases.

The invention discloses a method for purifying sialic acid from fermentation liquor, and belongs to the field of biochemical engineering separation and purification. The method comprises the following steps: firstly, concentrating sialic acid biological fermentation liquor by adopting membrane separation, adjusting the pH value of the concentrated liquor to 7-11, loading a sample on a boric acid affinity chromatography column, respectively washing with water and acid water, and collecting a sialic acid eluate; adjusting the pH value of the saliva acid eluate to 5-10, loading the saliva acid eluate to anion exchange resin, washing with water and acid water to obtain a resin desorption solution, and adding the resin desorption solution into a spray dryer for drying to obtain a sialic acid finished product. The stable five-membered ring complex is generated by utilizing the action of a boric acid group and a dihydroxyl structure in sialic acid. According to the method, the sialic acid is subjected to complexing under an acidic condition, molecules are eluted from a medium, selective separation of the sialic acid is realized, the sialic acid is further refined and purified by adopting anion exchange resin, high-purity sialic acid is prepared, introduction of a crystallization process is avoided, and use of an organic solvent and an acid reagent is greatly reduced.

The invention discloses cubilose sialic acid glycan peptide capable of improving lung inflammation and application of the cubilose sialic acid glycan peptide. The ratio of fucose modified sugar chains in the cubilose sialic acid glycan peptide is 30-60%, the ratio of sialic acid modified sugar chains in the cubilose sialic acid glycan peptide is 20-40%, the core structure of the fucose type is (HexNAc) 3 (Man) 3 (Fuc) 1, the core structure of the sialic acid type is (HexNAc) 3 (Man) 3 (Gal) 1 (Fuc) 1 (NeuAc) 1, the neutral sugar dissolution rate of the cubilose sialic acid glycan peptide is high, and the glycopeptide shown in SEQ ID NO 2 has a good inflammation improving effect.

The present disclosure relates compositions comprising milk fat globule membrane (MFGM) and a mixture of HMOs with at least one fucosylated HMO and at least one HMO selected from neutral core HMOs or sialylated HMOs. The compositions have potential health benefits in terms of improving brain health, immunity and recovery time following exercise, by increasing the formation of glutamic acid.

The invention provides a whitening composition containing N-acetylneuraminic acid and application thereof.The whitening composition comprises N-acetylneuraminic acid (sialic acid), nicotinamide, protein peptide and acerola cherry extract, and the mass of the protein peptide and the mass of the acerola cherry extract both do not exceed two times of the mass of sialic acid. The sialic acid, the nicotinamide, the protein peptide and the acerola cherry extract are reasonably combined, so that an excellent whitening effect can be achieved, particularly, the melanin relieving effect is obvious, the sialic acid can effectively prevent attenuation of effective components, and the browning and attenuation phenomena of the sialic acid can be stabilized by adding the nicotinamide.

The invention provides a quality control method for synthesizing 3 '-sialic acid lactose by an enzymatic process, which comprises the following steps: A) dissolving and extracting a test sample by using a solvent to obtain a to-be-detected solution; the test sample is a reaction solution or a finished product of 3 '-sialic acid lactose synthesized by enzyme catalysis; b) determining the liquid to be detected by adopting a high performance liquid chromatography to obtain an HPLC (High Performance Liquid Chromatography) spectrum of the 3 '-sialic acid lactose; according to the method, an HPLC-UV-RI combined detection method is adopted, the specificity is high, the sensitivity is high, the linear correlation is good, and the pretreatment process of the sample is simple and rapid; according to the method, the content of 3 '-lactulose sialylate, 6'-lactose sialylate sodium salt, D-lactose, CMP, CMP-SA and SA in the 3 '-SL process development process can be accurately detected, control over the conversion rate and impurities in the process is facilitated, effective detection of a product 3'-SL related substance-isomer can be achieved, and control over the product quality is facilitated.

The present invention discloses drug-loaded nanoparticles targeting neuraminidase, as well as a preparation method and application thereof, belonging to the technical field of pharmaceutical preparations. The present invention connects polysialic acid and polylactic acid glycolic acid via an ester bond to obtain drug-loaded nanoparticles with stable structure and performance. The drug-loaded nanoparticles have the function of targeting neuraminidase and have relatively high efficiency in entering cells. Furthermore, antibiotics are dispersed in the drug-loaded nanoparticles, and an inflammatory modulator is adsorbed on the surface of the drug-loaded nanoparticles. The drug-loaded nanoparticles can deliver the antibiotics and the inflammatory modulator to Grasseria suis in pigs, releasing the antibiotics and the inflammatory modulator in the slightly acidic environment of the infected site in the pig, thereby efficiently killing Grasseria suis and reducing inflammatory damage caused by bacterial infection and toxic damage of the antibiotics. Therefore, the present invention has good application prospects in the preparation of drugs for preventing and / or treating Grasseria suis infection.

The invention relates to a NeuC mutant, a host cell containing the NeuC mutant and a method for producing sialic acid by fermentation. The invention discloses a NeuC mutant, a coding nucleic acid thereof, a vector, a host cell containing the vector, and a method for producing sialic acid through fermentation by using the host cell. Compared with wild NeuC, the NeuC mutant can obviously improve the yield of sialic acid. The sialic acid yield can reach 61 g / L after high-density fermentation culture is performed for 60 h in a 3L fermentation tank.

The present invention relates to a fermented product containing a component X, a component Y, and a component Z, wherein the component X is sialic acid or a sialylated oligosaccharide, the content of the component X per solid content of the fermented product is 80 mass% or more, the component Y is acetic acid, the ratio (component Y content / component X content) of the content of the component Y to the content of the component X in the fermented product is 0.010 or less on a mass basis, the component Z is at least one of a component Z1 and a component Z2, the component Z is a component other than the component X, the component Z1 is a neutral sugar, and the component Z2 is an acidic sugar.

The invention discloses a sialic acid molecular imprinting extraction method, and belongs to the technical field of separation and purification. The invention discloses a sialic acid molecular imprinting extraction method which comprises the following steps: dissolving a sialic acid standard substance and a functional monomer in a binary pore-foaming agent-ionic liquid, adding a cross-linking agent and an initiator, polymerizing under ultraviolet light to prepare a high-molecular polymer, and washing off the sialic acid standard substance to prepare a molecular imprinting polymer; specifically adsorbing sialic acid in the fermentation liquor by using the molecularly imprinted polymer; performing graded elution on sialic acid by using a methanol-water mixed solution and a methanol-acetic acid mixed solution; and performing rotary evaporation concentration, anti-solvent crystallization, separation and drying on the bird's nest acid eluent. Tests show that the sialic acid purity is greater than or equal to 99%, and can meet the requirements of the sialic acid in the fields of food, health care, medicine, cosmetics and the like. According to the method, sialic acid can be efficiently separated and extracted from sialic acid fermentation liquor, the product yield is increased, the extraction procedures are reduced, and the production cost and the discharge amount of three wastes are reduced.

The present disclosure relates to a composition for balancing the immune system of a neonatal or infant subject, the composition comprising an effective amount of 2 '-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6 '-sialyllactose, and lactose-N-tetrasaccharide.

The invention discloses application of NANS in sialic acid induced vascular endothelial injury, and belongs to the technical field of biological medicine. The research shows that NANS is used as a key protease in endogenous synthesis of sialic acid. After the NANS gene is silenced, sialic acid level is reduced by inhibiting sialic acid endogenous synthesis, so that endothelial inflammation injury is relieved. The invention provides a new target for endothelial inflammation injury, so that a new treatment method is provided for development of drugs for treating endothelial injury vascular diseases, the treatment effect of the endothelial injury vascular diseases is improved from inhibition of sialic acid endogenous synthesis, and the purpose of eradicating the endothelial inflammation injury is achieved.

The present invention provides methods for glycosylation and compound preparation. The compounds include galactosylated, sialylated, fucosylated, and N-acetylglucosaminated compounds from simple oligosaccharides and sugars of animal, plant, or microbial origin. In certain embodiments, the invention provides for trinucleotide-free enzymatic production of oligosaccharides starting from simple sugars, including plant-based sugars. The present invention also provides methods of enzymatic production of fucosylated oligosaccharides and fucosylated antibody-glycan conjugates from ordinary enzymes. The production may be a cell-free, one-pot synthesis using an enzyme and, in some embodiments, an immobilized enzyme. The synthesis is a highly customizable and efficient cell-free manufacturing process. In some embodiments, lactose derivatives and human milk oligosaccharides (HMOs) are produced.

The invention discloses a nutritional composition containing phospholipids and sialic acid oligosaccharides and capable of promoting construction of a neural network. The invention provides a nutritional composition, the nutritional composition comprises the following necessary components: a phospholipid component and a sialic acid oligosaccharide component, and in the nutritional composition, the molar ratio of the phospholipid component to the sialic acid oligosaccharide component is (1-10): (0.01-5). The composition provided by the invention has the advantages that the components are synergistic, the construction of a neural network is effectively promoted, the application range is wide, and various foods or health-care products can be conveniently added or prepared.

The invention relates to the technical field of biological medicine, in particular to a cerium oxide nano-enzyme delivery system based on carried neutrophil, and the system comprises cerium oxide nano-particles; the surfaces of the cerium oxide nanoparticles are coated with the lipoic acid zwitterionic polymer layers; the sialic acid molecules and the protocatechuic aldehyde molecules are modified on the surface of the lipoic acid zwitterionic polymer layer; wherein the system realizes breakthrough of a blood brain barrier and precise delivery of ischemic lesions through mediation targeting delivery of L-selectin on the surface of neutrophile granulocyte, and based on a cerium oxide nano-enzyme delivery system carrying neutrophile granulocyte, a'antioxidation-anti-inflammation-angiogenesis promotion-neural restoration 'quadruple synergistic mechanism is integrated, so that the system has the advantages that the system is simple in structure and convenient to use, and the application prospect is wide. And a new strategy of multi-target intervention is provided for cerebral arterial thrombosis treatment.

The invention provides breast milk oligosaccharide for regulating butyric acid and improving intestinal microenvironment health and application thereof. Specifically, the invention provides an application of breast milk oligosaccharide in preparation of food for improving intestinal microenvironment health, and the breast milk oligosaccharide is one of fucosyl oligosaccharide, sialic acid oligosaccharide or lactose-N-tetrasaccharide. Wherein the improvement of the intestinal microenvironment health comprises the steps of regulating and controlling the production of butyric acid in an intestinal system, increasing the overall production of short-chain fatty acid, being utilized by intestinal flora as prebiotics in the intestinal system and producing gas, reducing the production of isobutyric acid and isovaleric acid and / or reducing the pH value to maintain the intestinal microenvironment health. The breast milk oligosaccharide is used for being added into infant food (including infant formula powder, complementary food and nutritional supplements) and nutritional supplements or food for children, teenagers and adults over 3 years old, and has a wide application prospect.

The invention belongs to the field of synthetic biology and metabolic engineering, and particularly relates to high-yield sialyl acyl-N-tetrasaccharide a escherichia coli as well as a construction method and application thereof. According to the method, recombinant escherichia coli is taken as an initial strain, and a beta1, 3-galactosyl transferase gene wbgO from escherichia coli O55: H7 is integrated into a chromosome in a multi-copy form, so that a lactyl-N-tetrasaccharide efficient synthesis chassis strain without plasmid dependence is constructed. And overexpression neuC, neuB, neuA and Nm3ST are introduced through plasmids, so that the supply of glycosyl donors is improved. The expression intensity of uridine diphosphate-N-acetylglucosamine synthesis pathway genes glmM and glmUS is regulated through ribosome binding site engineering, and a cytidine triphosphate regeneration module is introduced, so that the yield of sialyloyl-N-tetrasaccharide a is increased. And finally, the maximum titers of the sialyloyl-N-tetrasaccharide a respectively reach 2.238 g / L and 8.178 g / L by shaking a flask and culturing in batches. The invention provides an efficient technical scheme for industrial production of complex breast milk oligosaccharide.

The invention belongs to the technical field of nutritional compositions, and particularly relates to a composition, a preparation method thereof and application of the composition in protecting brain health. The composition provided by the invention is prepared from docosahexaenoic acid, 3-sialic acid lactose and phosphatidylserine. The composition is clear in sleep improvement effect, can effectively solve the problem of sleep maintenance caused by sleep deprivation, has the effects of protecting neural functions, improving memory and relieving anxiety from multiple dimensions, and can repair brain tissue damage and protect brain health. Based on the composition, products for assisting in improving memory, preventing and treating insomnia and anxiety, regulating neurodevelopmental disorders, protecting brain health and improving concentration can be developed, the health requirements of different people are comprehensively met, and the application value is high.

The present invention relates to a method for the production of a sialylated glycosphingolipid, the method comprising the steps of: ─ providing a glycosphingolipid, a sialic acid donor, and an enzyme having a trans-sialidase activity, ─ mixing said glycosphingolipid with said sialic acid donor in the presence of said enzyme having trans-sialidase activity, thereby producing said sialylated glycosphingolipid, and wherein the sialic acid donor is 3´-sialyllactose.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention relates to the technical field of biomedical engineering and drug delivery, and particularly discloses a novel targeted drug delivery system based on rose fruit extracellular vesicles, which comprises the following steps: preparing rose fruit source extracellular vesicles; preparing a medicine carrying vesicle; preparing targeted modified drug-loaded vesicles; according to the application disclosed by the invention, a stem related pathway is specifically down-regulated through the components of the rose fruits, and meanwhile, conventional tumor cells are killed by using adriamycin, so that a complementary treatment mechanism is formed, and the tumor recurrence risk is fundamentally reduced. The phenylboronic acid group of DSPE-PEG-PBA is used for specifically recognizing sialic acid over-expressed on the surface of a tumor cell, so that the enrichment of the medicine in tumor tissues is remarkably improved, and meanwhile, the distribution of normal tissues is reduced. By means of the natural biocompatibility and low immunogenicity of the RHNVs and in combination with the long circulation characteristic of a PEG chain, the delivery efficiency is guaranteed, the systemic toxicity is reduced to the maximum extent, and particularly the cardiotoxicity and myelosuppression of adriamycin are relieved.

The invention relates to the field of biological medicine, in particular to an Mmrn1 targeted inhibitor, an inhibition method and application. According to the application disclosed by the invention, firstly, the relationship between the expression of the MMRN1 gene in the acute myelogenous leukemia (AML) and the regulation and control of a'siglecan-siglecc 'axis is creatively disclosed. Therefore, the invention firstly provides a small interfering RNA (Ribonucleic Acid) targeting the Mmrn1 in the leukemia stem cell, and an application of the small interfering RNA in preparation of a'sialic acid glycan-siglecc 'axis immune checkpoint inhibitor.