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This invention discloses a control method and system for a high-temperature coating furnace for pyrolytic graphite, belonging to the field of material preparation and high-temperature heat treatment technology. It solves the problems of poor deposition uniformity, contradiction between deposition efficiency and quality, and poor process repeatability that exist in coating furnace control methods when preparing workpieces. The technical solution includes the following steps: The substrate to be deposited is placed in the furnace chamber of the high-temperature coating furnace for pyrolytic graphite and pretreated; the pretreated substrate is subjected to multi-stage variable temperature and flow rate deposition control to complete the deposition operation. The multi-stage variable temperature and flow rate deposition control includes four sub-stages performed sequentially: preheating and degassing, interface nucleation, stable growth, and final densification; after the deposition operation is completed, the substrate is cooled, and the substrate coated with pyrolytic graphite is removed. This invention is applied to coating furnaces.

The application discloses a DF-1 cell line with PML gene knocked out and application thereof, and comprises the following steps: designing sgRNA which is specific to a chicken-derived PML gene; constructing a knockout plasmid containing a Cas9 protein gene and sgRNA which is specific to the chicken-derived PML gene; performing cell transfection, achieving gene silencing by using a CRISPR-Cas9 system, and then obtaining chicken-derived PML knockout cells through subcloning. The application successfully obtains the DF-1 cell line with the chicken-derived PML gene knocked out by CRISPR / Cas9 gene editing, and finds that knocking out the PML can promote CIAV replication. The application provides important biological materials for efficiently amplifying CIAV and preparing a low-virulence vaccine, lays a foundation for exploring the biological function of the chicken-derived PML and analyzing the role and mechanism of the PML in CIAV and other virus infection and replication, and has a good application prospect.

This invention belongs to the fields of genetic engineering and fish molecular breeding technology, specifically relating to the application of ARMH3 inhibitors in the preparation of antiviral drugs for fish or the creation of hemorrhagic disease-resistant fish germplasm. This invention discloses that ARMH3 is an important host factor for grass carp reovirus infection. The amino acid sequence of ARMH3 is shown in SEQ ID NO.1. ARMH3 is utilized by grass carp reovirus, promoting viral inclusion body formation, thereby facilitating viral replication and infection. This invention utilizes inhibitors of the ARMH3 protein encoded in grass carp... ARMH3 Genes were edited using CRISPR / Cas9 to create... ARMH3 Knockout chimeric grass carp populations exhibit significant resistance to hemorrhagic disease. This invention provides molecular targets and methods for the rapid creation of new grass carp germplasm resistant to hemorrhagic disease.

The application discloses a kind of compatible multi-specification wire's exchange shear integrated device and its use method, including frame body;Wire shearing guide rail, wire shearing guide rail is set on frame body, wire shearing guide rail is matched with wire shearing guide rail sliding block;Tool support, tool support is connected with wire shearing guide rail sliding block, and tool support is equipped with shearing cutter;Ball screw pair, ball screw pair is set on frame body, and ball screw pair is connected with ball screw motor;Push-pull cylinder, push-pull cylinder is set on connecting plate;Linear guide rail, linear guide rail is set on connecting plate;Wire storage limiting block, wire storage limiting block is located on linear guide rail sliding block and is connected with push-pull cylinder, and wire storage limiting block is equipped with wire stop post, wire feeding roller, wire length measuring wheel, front end guide column and rear end guide column;Mini electric cylinder, mini electric cylinder is connected with wire stop post.It has the advantages of high integration, small space occupation, high standardization, strong flexibility, and meets the needs of different specifications and sizes of wire feeding and shearing.

The application relates to an upflow electro-catalytic gas floatation device and a wastewater treatment method. The device comprises, from bottom to top, an electro-catalytic oxidation three-dimensional electrode filler layer, a water outlet pipeline, a micro-nano bubble distribution plate, a water inlet distribution pipeline and a skimmer arranged in a treatment chamber. The device further comprises an internal reflux pipeline, which comprises an upper reflux section and a lower reflux section. An upper reverse cone and a lower reverse cone for separating floatage and wastewater are respectively arranged on the upper reflux section and the water outlet pipeline. The lower reflux section guides the wastewater separated by the upper reverse cone to the lower part of the electro-catalytic oxidation three-dimensional electrode filler layer. The device has high integration, small floor area, high wastewater treatment efficiency, and can improve the electro-catalytic oxidation efficiency and reduce the equipment operation and maintenance cost.