The embodiment of the invention discloses a
magnetic separation chip for
cell separation and a preparation method thereof.The
magnetic separation chip structurally comprises a sample layer, a functional layer, a clamp set and a
magnetic field source, the sample layer is composed of a flow channel layer and a sealing film, and the flow channel layer is provided with a sheath fluid inlet, a sample inlet and a sample outlet; the sealing film is attached to the
open surface of the runner layer to seal the runner; the functional layer is located below the sample layer and comprises a
patterned substrate and an amorphous
alloy soft magnetic strip material, the
patterned substrate is provided with a counter bore groove, the amorphous
alloy soft magnetic strip material is embedded into the counter bore groove, and the upper surface of the amorphous
alloy soft magnetic strip material is flush with the upper surface of the substrate, right faces the sealing film and is arranged in the extending direction of the flow channel; the clamp group comprises an upper clamping plate and a lower clamping plate, the upper clamping plate is arranged above the sample layer, the lower clamping plate is arranged below the functional layer, the upper clamping plate is provided with runner holes corresponding to the inlets and the outlets, and the two are connected through bolts to clamp and fix the sample layer and the functional layer; the
magnetic field source is arranged at the end where the sample outlet is located and on one side opposite to the functional layer, so that a gradient
magnetic field which is gradually enhanced from the inlet to the outlet is formed in the flow channel.