99 results about "Culture cell" patented technology

The present invention relates to a polymer conjugate comprising a ULA polymer a) and one or more polypeptides b), wherein the polypeptides are conjugated to the ULA polymer, and the conjugate is coated on the surface of a device for culturing cells. The peptides can interact with receptors on the surface of the cells, while the polymer prevents non-specific binding of the cells to the surface of the device.

The invention belongs to the field of veterinary virology, and discloses a bovine ephemeral fever virus strain and reverse genetic application thereof, and the reverse genetic steps are as follows: taking pCI plasmid as a skeleton to prepare plasmid for expressing a complete genome of bovine ephemeral fever virus; the method comprises the following steps: respectively preparing auxiliary plasmids for expressing an N protein gene, a P protein gene, an L protein gene and a G protein gene of the bovine ephemeral fever virus by taking pCAGGS plasmids as a skeleton, co-transfecting BHK-21 cells by using the five plasmids, and culturing the cells to obtain the rescued bovine ephemeral fever virus. The rescued bovine ephemeral fever virus has no obvious difference from the reproductive capacity of the parent virus, and the genome is still stable after 10 generations of continuous passage in BHK-21 cells. The invention provides methodological support for genetic modification of the bovine ephemeral fever virus, and has an application prospect.

An object of the present invention is to provide a cell culture method and a production method for a product, in which an occurrence of aggregates in foam is suppressed.According to the present invention, there is provided a cell culture method of culturing cells at a cell density of 30×106 cells / mL or more and 400×106 cells / mL or less in a culture solution, the method including: step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution; or step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower.

The present invention provides an inbred corn line designated JRDJ6933, methods for producing a corn plant by crossing plants of the inbred line JRDJ6933 with plants of another corn plant. The invention further encompasses all parts of inbred corn line JRDJ6933, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line JRDJ6933, and plants produced according to these methods.

One approach to the exploration method includes calculating a TAD score representing transcriptional activity for each TAD present in the genome and selecting TADs based on the TAD scores. Another approach includes analyzing a genome obtained from cells expressing a foreign gene and selecting the boundaries of regions containing the foreign gene. Cell preparation involves inserting the target gene into the regions identified by the exploration method. Cell sorting involves calculating a TAD score representing transcriptional activity for each TAD present in the genome, selecting TADs based on the TAD scores, and selecting cells containing the target gene in the selected TADs. The cells are derived from Chinese hamsters and are cells in which the target gene has been inserted into at least one specified region. The method for manufacturing the cell product includes culturing the cells to express the target gene.

The present invention provides an inbred corn line designated KFF7351, methods for producing a corn plant by crossing plants of the inbred line KFF7351 with plants of another corn plant. The invention further encompasses all parts of inbred corn line KFF7351, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line KFF7351, and plants produced according to these methods.

The utility model relates to the technical field of cell culture, and discloses a cell shake flask capable of improving tissue digestion efficiency, which comprises a culture flask body, a flask cover is mounted at the top end of the culture flask body, and a first constant-temperature heating cover is arranged on one side of the outer side wall of the culture flask body. Mounting blocks are fixed to the front end and the rear end of one side of the first constant-temperature heating cover correspondingly, inner cavities are formed in the first constant-temperature heating cover and the second constant-temperature heating cover correspondingly, and electric heating wires are mounted in the inner cavities. When the culture bottle body disclosed by the utility model is used for culturing cells, the culture bottle body can be heated according to actual conditions by arranging components such as the first constant-temperature heating cover, the mounting block, the mounting groove and the second constant-temperature heating cover, and meanwhile, the digestion efficiency of cell tissues can be promoted by heating the culture bottle body; and the culture bottle body can be detached when not needed subsequently, so that the observation of the condition of cells in the culture bottle body through the culture bottle body is not influenced, and the use effect is better.

The invention relates to the field of cell culture. The invention particularly relates to culturing cells, such as fibroblasts, to obtain collagen sheets. Modified scaffolds have been found to improve the characteristics of the method. The invention also relates to a collagen sheet and to leather obtained by tanning such a collagen sheet.

The present invention provides an inbred corn line designated BGID2926, methods for producing a corn plant by crossing plants of the inbred line BGID2926 with plants of another corn plant. The invention further encompasses all parts of inbred corn line BGID2926, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line BGID2926, and plants produced according to these methods.

This invention relates to the field of cell biology, specifically to a co-culture cell model and method for primary glial cells and neurons. The method for co-culturing primary glial cells and neurons provided by this invention includes the following steps: a mixture of primary glial cells and neurons is seeded into a first co-culture medium for pre-culture; the medium is discarded and the cells are washed; a second co-culture medium is added for a first-stage culture; after the first-stage culture, a second-stage culture is performed; the medium used in the second-stage culture is a third co-culture medium supplemented with macrophage colony-stimulating factor; after the second-stage culture, a third-stage culture is performed; the medium used in the third-stage culture is the second co-culture medium; in the obtained co-culture cell model, microglia are differentiated and mature, and are in a quiescent state, thus obtaining a primary glial cell and neuron co-culture model.

A substrate for culturing cells that comprises a modified prolamin having a net positive charge is provided. The modified prolamin may be a prolamin that has been modified through amidation or esterification, such as the addition of a methyl-ester group or an ethanolamine group. The modified prolamin is animal-free may be food-grade and / or may be free of cross-linkers. The modified prolamin may be in a coating that can be applied to a surface for culturing adherent cells, such as a microcarrier (e.g., beads) or well plates. Substrates coated with the modified prolamin are able to support cell expansion and can be used for food-grade applications such as cultured meat.

A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.

The application discloses a kind of mixed immune cells and its culture system and in vitro culture method.The mixed immune cells include 75%~80% of γδT cell, 10%~15% of NK cell and 1~2% of NKT cell.The in vitro culture method of the application includes, when peripheral blood mononuclear cell is inoculated with activation culture medium, adding immune cell first activation component, adding immune cell reactivation component after 0~6 hours of inoculation, continuous activation culture;Then the activated culture cell is transferred into the coated culture plate or culture bottle, and the mixed immune cell is obtained by expansion culture.The mixed immune cell of the application has higher liver cancer cell HepG2 killing effect.The mixed immune cell in vitro culture method of the application has high expansion efficiency, and the expansion multiple can reach 2000-8000 times.

The present invention provides an inbred corn line designated BGFA3005, methods for producing a corn plant by crossing plants of the inbred line BGFA3005 with plants of another corn plant. The invention further encompasses all parts of inbred corn line BGFA3005, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line BGFA3005, and plants produced according to these methods.

This invention provides a method for producing PD-1 antibodies with high protein expression levels, high antibody activity, and / or high galactosyl levels. This method involves culturing cells in a culture medium until the target protein is obtained. The culture temperature is 37°C, which is then lowered to 33-34°C on the 5th day of culture. The culture medium also contains additives, namely galactose and manganese chloride. The concentrations of galactose and manganese chloride added are 0.05-6 mM and 0.05-3 μM, respectively. The culture medium consists of a basal medium and a feed medium. The basal medium is Star CHO, and the feed medium is Star CHO Feed and CDFS36. The basal medium also contains 1 g / L poloxamer. The Star CHO Feed feed is added at 4% / 5% / 6% / 6% / 5% / 4% of the initial culture volume each time, and the CDFS36 feed is added at 0.4% / 0.5% / 0.6% / 0.6% / 0.5% / 0.4% of the initial culture volume each time. The additives galactose and manganese chloride, as well as the Star CHO feed feed... Feed and CDFS36 were added every 2 days starting from day 3 of culture; the dissolved oxygen in the culture system was 40%, and the pH was 7.0 ± 0.1; the rotation speed of the culture system was 240 rpm.

The application provides an isolated circular nucleic acid molecule and a pharmaceutical composition.The isolated circular nucleic acid molecule has a nucleotide sequence shown in SEQ ID NO:1; the pharmaceutical composition comprises: an agent for inhibiting the expression or activity of the circular nucleic acid molecule, wherein the circular nucleic acid molecule has a nucleotide sequence shown in SEQ ID NO:1.The isolated circular nucleic acid molecule plays an important role in regulating the development of cancer, and therefore, the pharmaceutical composition can effectively promote the apoptosis and death of lung cancer cells, for example, cancer; or, when culturing cells in vitro, the pharmaceutical composition can be used to promote the apoptosis of cancer cells.

The invention relates to the technical field of biomedical treatment, and particularly provides a cell culture workstation. Specifically, the cell culture workstation comprises a box body which is provided with a refrigerating chamber and a culture chamber; the first liquid storage component is used for storing a culture solution and is positioned in the refrigerating chamber; an incubator capable of storing cells and culturing the cells and located in the culture chamber; the liquid supply assembly is used for conveying the culture liquid in the first liquid storage component into the incubator; and the driving device can drive the incubator to rotate. The cell culture work station is provided with the refrigerating chamber, the culture solution is stored in the first liquid storage component of the refrigerating chamber, the culture solution can be automatically conveyed into the culture device through the liquid supply assembly, the risk of cell culture pollution can be reduced, in addition, the cell culture work station is additionally provided with the driving device, and in the cell culture process, the driving device is driven by the driving device. The incubator can be driven by the driving device to rotate, so that a culture solution in the incubator is more uniform, and the growth of cells is facilitated.

The invention relates to the technical field of computational toxicology, and discloses a method for calculating combination of perfluorinated and polyfluorinated alkyl compounds and PPARalpha based on a pharmacophore model, which comprises the following steps: acquiring a PFAS initial data set, acquiring a PPARalpha ligand binding domain crystal structure, and optimizing the structure; selecting representative PFAS to carry out molecular docking, and carrying out simulated annealing optimization to obtain an optimal binding posture; then generating a pharmacophore model; the models are screened; performing rigid fitting screening by using a pharmacophore model; carrying out molecular dynamics simulation on the representative hit object, and verifying the stability; protein is extracted by culturing cells in vitro, a thermal displacement experiment is carried out, and the thermal stability effect of PFAS on PPARalpha is verified. According to the method, comprehensive virtual screening of PFAS-PPAR alpha interaction based on pharmacophores is provided, a calculation framework based on protein and compound structures is established, and the method is suitable for environmental pollutant screening and safer chemical design.

Improved methods for large scale production of proteins and / or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

The methods and compositions disclosed herein relate to the field of cell therapy, and more specifically, to improving CAR and / or TCR function through improvement of the tumor microenvironment via improvement in cytokine signaling. Methods of treating a cancer in a patient are disclosed comprising administering to the patient immune cells expressing a chimeric antigen receptor or T-cell receptor and a membrane bound IL-18. Further disclosed are nucleic acids which encode a membrane bound IL-18 and methods for culturing cells expressing such nucleic acids.

The present invention provides: a serum replacement composition for cell culture, comprising an extract of Chlorella protothecoides; and a method for culturing cells, such as muscle stem cells, using the composition.

A method for determining the relationship between a plurality of substances and cells, comprising: providing a first droplet, a second droplet, a third droplet, and a micro-well array chip, the micro-well array chip having a plurality of micro-well combinations, each micro-well combination comprising a large micro-well (110) and a plurality of small micro-wells (120) adjacent to and in communication with the large micro-well (110), the pore size of the large micro-well (110) being larger than the pore size of the small micro-well (120) (S100); first adding the second droplet into the micro-well array chip and into the large micro-well (110), and then adding the first droplet into the micro-well array chip and into the plurality of small micro-wells (120) (S200); allowing the first droplet and the second droplet to fuse, culturing cells in the micro-well array chip (S300); after the cell culture is completed, adding the third droplet into the empty small micro-well (120), the third droplet fusing with the first fused droplet after cell culture to obtain a second fused droplet (S400); demulsifying the second fused droplet, collecting magnetic beads, constructing a library and sequencing the nucleic acid carried on the magnetic beads, and determining the relationship between the plurality of substances and the cells based on the sequencing results (S500).

The present invention provides a method for producing a target protein by culturing cells, the method being capable of stably maintaining a target plasmid without using an antibiotic. The present invention relates to a method for producing a target substance, the method including a step for culturing cells that are transformed with a target plasmid (A), wherein: each of the transformed cells includes the target plasmid (A) and a temperature-sensitive plasmid (B) and are obtained by a method that includes a step for culturing cells in each of which the function of an essential gene on the chromosome has been reduced at a temperature at which the temperature-sensitive plasmid (B) is eliminated; the target plasmid (A) includes the essential gene and a target substance gene and does not include an antibiotic-resistant gene; and the temperature-sensitive plasmid (B) includes the essential gene and the antibiotic-resistant gene.

This article is a container for culturing cells. As shown in the reference perspective view illustrating the names of its parts, this article has a structure in which multiple wells and openings are provided on a plate. In this article, one unit consists of four first wells arranged in a matrix, and second wells and openings positioned between the four first wells. As shown in the front view, the four first wells, second wells, and openings of one unit are connected by channels formed within the plate. This article is provided with, for example, four such units. In each unit, this article allows liquids containing culture medium or cells to flow in and out of the channels formed in the plate through the first wells, second wells, and openings. This article can also be used as part of a cell culture container with a two-dimensional arrangement of unit structures by arranging multiple articles in a case. For example, by arranging six articles in parallel, a cell culture container with 4 × 6 units can be constructed.

The invention relates to the field of bioengineering, and discloses an establishment method of a high and low liver cancer matrix hardness experimental culture system. The method comprises the following steps: synthesis of RGD-SA, preparation of a CaCl2 solution, preparation of RGD-SA hydrogel, cell culture and recovery of RGD-SA hydrogel cells. According to the experimental culture system, on the basis that sodium alginate and calcium ions are subjected to chemical reaction to form calcium alginate, the hardness of three-dimensional hydrogel is adjusted by adjusting the concentration of the calcium ions, meanwhile, RGD is used for modifying SA so as to enhance and optimize the adhesion and hardness sensing ability of cells to the hydrogel, liver cancer cells are cultured in an RGD-SA hydrogel system, and the survival rate of the liver cancer cells is increased. In-vitro simulation of three-dimensional growth states of liver cancer cells in normal-hardness liver and high-hardness liver environments is well realized, and an ideal novel experimental research platform is provided for understanding liver cancer pathological characteristics and analyzing a hardness mechanical induction signal transmission process mechanism from the biomechanical perspective.

The invention provides a preservation and culture method suitable for in-vitro tissue of a cell line, which comprises the following steps: firstly, cleaning the in-vitro tissue by using PBS (Phosphate Buffer Solution) containing double antibodies, scraping foreign matters on the surface, and then respectively cleaning twice by using 75% alcohol and PBS containing double antibodies to obtain a pretreated sample; then, the pretreated sample is loaded into a sterile sample bag and is vacuumized, and the sterile sample bag is placed in a precooling biological safety transport box, so that long-time and long-distance transport is realized, and cell activity is maintained; and finally, carrying out somatic cell separation and culture on the pretreated sample to obtain primary cells. According to the method, through combination of vacuum treatment and preservation at 4 DEG C, oxidative damage and autolysis of tissues are effectively delayed, and the risks that genetic resources of precious somatic cells are polluted and line establishment fails under difficult conditions such as remote areas are remarkably reduced; through verification, the line can still be successfully established after the tissue is preserved for 14 days, and cells can be stably passaged for 20 generations in vitro in cooperation with an optimized culture medium.

The present invention provides an inbred corn line designated TPCD7929, methods for producing a corn plant by crossing plants of the inbred line TPCD7929 with plants of another corn plant. The invention further encompasses all parts of inbred corn line TPCD7929, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line TPCD7929, and plants produced according to these methods.

The present invention provides an inbred corn line designated CCJC6735, methods for producing a corn plant by crossing plants of the inbred line CCJC6735 with plants of another corn plant. The invention further encompasses all parts of inbred corn line CCJC6735, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line CCJC6735, and plants produced according to these methods.

The present invention provides an inbred corn line designated BFJD6621, methods for producing a corn plant by crossing plants of the inbred line BFJD6621 with plants of another corn plant. The invention further encompasses all parts of inbred corn line BFJD6621, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line BFJD6621, and plants produced according to these methods.

Embodiments of methods, systems, and devices are disclosed for screening of cells for chirality. The methods include seeding and culturing cells onto a substrate having microgrooves, collecting images of the cells on the substrate, and performing an image analysis to determine the angular distribution of the cells with respect to the microgrooves. For some embodiments, the image analysis involves the use of fast Fourier transforms to determine the angular distribution. The methods, systems and devices provide improved means for rapidly evaluating cellular chirality.