169 results about "Culture cell" patented technology

The invention discloses a cat kidney suspension culture cell line suitable for serum-free suspension culture and application of the cat kidney suspension culture cell line. The name of the cell line is F81-B4, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46350. After 50 generations of continuous subculture, the cells still can maintain the original proliferation level and cellular morphology, the cell number can reach 9.0 * 10 < 6 > / mL after the cells are subcultured at the density of 1.0 * 10 < 6 > / mL for three days, the multiplication time is 22-26 hours, and the cell proliferation speed is equivalent to that in a shake flask when the cells are amplified to a 10L bioreactor for culture. The cell line is highly susceptible to feline parvovirus, feline calicivirus, feline herpes virus, canine parvovirus and canine distemper virus, the virus content of a virus solution harvested 72 h after FPV virus inoculation can reach 107.5 TCID50 / mL, the virus content of a virus solution harvested 24 h after FCV virus inoculation can reach 1010.8 TCID50 / mL, the virus content of a virus solution harvested 48 h after FHV-1 virus inoculation can reach 107.5 TCID50 / mL, compared with an existing F81 adherent cell production process, the production process has the advantages that the production efficiency is high, and the production cost is low. The method has the characteristics of rapid lesion, short virus collection time and high titer. The cell matrix is an ideal cell matrix which can be used for culturing the three viruses.

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The invention discloses a cfDNA detection standard substance and a preparation method thereof. The preparation method comprises the following steps: carrying out multiplication culture on cells; performing cell apoptosis treatment on the amplified cells; after cell apoptosis treatment is finished, incubating for a period of time, and collecting supernate; adding the supernate into plasma to prepare a plasma standard substance for cfDNA detection; or extracting cfDNA in the supernate, and preparing a DNA standard substance for cfDNA detection; according to the preparation method, the supernatant of a culture cell line is adopted as a source of a cfDNA detection standard substance, rapid death of cells is promoted through a method of nutrient depletion or cytotoxic reagent addition, the content of mononuclear bodies in the supernatant of the culture medium is increased, the concentration of cfDNA is further increased, and the preparation method is easy to operate, high in yield and low in cost.

The invention relates to a co-culture microsphere model with a core-shell structure and a preparation method of the co-culture microsphere model. The co-culture microsphere model comprises a core-shell microsphere, the core-shell microsphere is composed of an inner core and an outer shell, the inner core is an organoid loaded by matrigel, the outer shell is formed by crosslinking a photo-crosslinking hydrogel material, co-culture cells are doped in the photo-crosslinking hydrogel material, and the inner core is wrapped by the outer shell. According to the co-culture microsphere model, double-layer structure microspheres of co-culture cell wrapped organoids are formed, immune infiltration and in-vivo characteristics can be simulated, and a similar in-vivo three-dimensional tumor immune microenvironment is provided. And the types of cells loaded on the inner layer and the outer layer can be flexibly replaced to form a microsphere model for co-culture of single or multiple cells and organoids. The co-culture microsphere model is more stable in structure, the size of the diameter of the core-shell microsphere can be optimized by changing 3D printing parameters, the operability of subsequent research is high, and the co-culture microsphere model is suitable for different research application scenes.

The present invention relates to a polymer conjugate comprising a ULA polymer a) and one or more polypeptides b), wherein the polypeptides are conjugated to the ULA polymer, and the conjugate is coated on the surface of a device for culturing cells. The peptides can interact with receptors on the surface of the cells, while the polymer prevents non-specific binding of the cells to the surface of the device.

The invention belongs to the field of veterinary virology, and discloses a bovine ephemeral fever virus strain and reverse genetic application thereof, and the reverse genetic steps are as follows: taking pCI plasmid as a skeleton to prepare plasmid for expressing a complete genome of bovine ephemeral fever virus; the method comprises the following steps: respectively preparing auxiliary plasmids for expressing an N protein gene, a P protein gene, an L protein gene and a G protein gene of the bovine ephemeral fever virus by taking pCAGGS plasmids as a skeleton, co-transfecting BHK-21 cells by using the five plasmids, and culturing the cells to obtain the rescued bovine ephemeral fever virus. The rescued bovine ephemeral fever virus has no obvious difference from the reproductive capacity of the parent virus, and the genome is still stable after 10 generations of continuous passage in BHK-21 cells. The invention provides methodological support for genetic modification of the bovine ephemeral fever virus, and has an application prospect.

The present invention relates to a method for three-dimensional culture and induced differentiation of neural organoids and use thereof. The method for three-dimensional culture and induced differentiation of neural organoids comprises: adding a signal pathway inhibitor into an induced pluripotent stem cell three-dimensional culture, and culturing cells to obtain the neural organoids. The signal pathway inhibitor comprises a TGF-β signal pathway inhibitor, a WNT signal pathway inhibitor, and a BMP signal pathway inhibitor. Compared with a traditional method, the neural organoids obtained by the method of the present invention feature a large size, small individual difference, and short culture time. The method is simple and rapid, and has high stability and low cost, and only a common incubator is needed. The method has wide application prospects.

To provide a culture vessel by which cells can be easily observed.SOLUTION: In a method for manufacturing a culture vessel, a culture vessel having a body part which has a through hole, and a sheet stuck on a lower face of the body part so as to close a lower end part of the through hole is manufactured. The method includes the steps of: adding tensile force to a sheet material with a thickness dimension of less than 50 μm; obtaining a first intermediate body in which the sheet material is stuck on a substrate; and sticking a surface of the sheet material side of the first intermediate body to the lower face of the body part.SELECTED DRAWING: Figure 2

This product is a cell manufacturing device that processes and cultures cells by setting a cartridge containing blood and reagents into the main body.

An object of the present invention is to provide a cell culture method and a production method for a product, in which an occurrence of aggregates in foam is suppressed.According to the present invention, there is provided a cell culture method of culturing cells at a cell density of 30×106 cells / mL or more and 400×106 cells / mL or less in a culture solution, the method including: step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution; or step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower.

The invention relates to a pueraria flower quality control and evaluation method based on reactive oxygen species (ROS) detection, and can effectively solve the problem that an existing pueraria flower quality detection method is difficult to reflect the overall efficacy of pueraria flowers. The method comprises the following steps: preparing a pueraria flower alcohol extract, constructing an alcohol damage cell model, preparing a cell culture medium containing gradient pueraria flower extract, culturing cells, and carrying out ROS level detection and statistics. The invention relates to a control group, a model group and a kudzuvine flower extract group, the internal quality of the control group, the model group and the kudzuvine flower extract group are jointly controlled from multiple angles, the current quality control and evaluation method is improved, a new technical approach can be provided for quality control and quality evaluation research of other alcohol effect dispelling and liver protecting traditional Chinese medicine decoction pieces, and the method is easy to operate, high in practicability, wide in application range and easy to popularize. The method is an innovation in traditional Chinese medicine quality control and evaluation.

The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and / or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and / or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.

The present invention provides an inbred corn line designated JRDJ6933, methods for producing a corn plant by crossing plants of the inbred line JRDJ6933 with plants of another corn plant. The invention further encompasses all parts of inbred corn line JRDJ6933, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line JRDJ6933, and plants produced according to these methods.

One approach to the exploration method includes calculating a TAD score representing transcriptional activity for each TAD present in the genome and selecting TADs based on the TAD scores. Another approach includes analyzing a genome obtained from cells expressing a foreign gene and selecting the boundaries of regions containing the foreign gene. Cell preparation involves inserting the target gene into the regions identified by the exploration method. Cell sorting involves calculating a TAD score representing transcriptional activity for each TAD present in the genome, selecting TADs based on the TAD scores, and selecting cells containing the target gene in the selected TADs. The cells are derived from Chinese hamsters and are cells in which the target gene has been inserted into at least one specified region. The method for manufacturing the cell product includes culturing the cells to express the target gene.

To provide safe and high-performance stem cells appropriate for clinical application, and a method for producing the stem cells.SOLUTION: Provided are a stem cell population derived from human deciduous tooth dental pulp, wherein 90% or more of the stem cell population is characterized by being CD117-negative, CD73-positive, CD90-positive, and CD105-positive, and a method for producing a stem cell population derived from human deciduous tooth dental pulp, the method comprising a step of culturing cells isolated from human deciduous tooth dental pulp in a medium that does not contain FBS (fetal bovine serum) in the presence of human platelet lysate (hPL).SELECTED DRAWING: None

Methods are disclosed for producing a polypeptide comprising an amino acid sequence including a lysine residue susceptible to hydroxylation, and / or including a proline residue susceptible to hydroxylation, comprising culturing cells comprising nucleic acid for expressing the polypeptide for the majority of the period of culture in cell culture medium having an Fe ion concentration of less than 60 µM.

The application provides a method for preparing FFPE quality control products by using cell lines, a reference product and a kit, and belongs to the fields of medicine, clinical laboratory science and biotechnology, and comprises the following steps: culturing cell lines by using microcarriers; mixing different cell lines based on requirements; centrifuging the mixed cell microcarrier suspension in a sealed malt pipe at one end; removing supernatant after the treatment; packaging the two ends of the malt pipe by using a semi-permeable membrane; removing the semi-permeable membrane after fixing, dehydrating and transparentizing the malt pipe by using a formaldehyde solution; separating the cell microcarriers after the wax immersion treatment from the malt pipe; and embedding the cell microcarriers into a paraffin block by using wax. The quality control product can be used in subsequent quality control processes, the mutation frequency of the quality control product can be kept in good uniformity, and batch production and large-scale production of the quality control product can be realized.

An apparatus for culturing cells includes a bioreactor with a fluid reservoir and a fixed bed, as well as a portion associated with the fluid reservoir and adapted for preventing cells from settling therein. The bioreactor may include a first vessel including the fluid reservoir and a second vessel with the fixed bed for the cell culture, which may be directly connected to the first vessel by a frangible connection to facilitate disassembly, such as for sampling. A conduit may be provided for connecting the first vessel to the second vessel, so as to establish fluid communication there between. A pump may also be provided for pumping fluid from the first vessel to the second vessel, such as though the conduit if present. Use of the apparatus as a screening tool for a large-scale bioreactor, in a kit, and related methods are also disclosed.

The present invention provides an inbred corn line designated KFF7351, methods for producing a corn plant by crossing plants of the inbred line KFF7351 with plants of another corn plant. The invention further encompasses all parts of inbred corn line KFF7351, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line KFF7351, and plants produced according to these methods.

The utility model relates to the technical field of cell culture, and discloses a cell shake flask capable of improving tissue digestion efficiency, which comprises a culture flask body, a flask cover is mounted at the top end of the culture flask body, and a first constant-temperature heating cover is arranged on one side of the outer side wall of the culture flask body. Mounting blocks are fixed to the front end and the rear end of one side of the first constant-temperature heating cover correspondingly, inner cavities are formed in the first constant-temperature heating cover and the second constant-temperature heating cover correspondingly, and electric heating wires are mounted in the inner cavities. When the culture bottle body disclosed by the utility model is used for culturing cells, the culture bottle body can be heated according to actual conditions by arranging components such as the first constant-temperature heating cover, the mounting block, the mounting groove and the second constant-temperature heating cover, and meanwhile, the digestion efficiency of cell tissues can be promoted by heating the culture bottle body; and the culture bottle body can be detached when not needed subsequently, so that the observation of the condition of cells in the culture bottle body through the culture bottle body is not influenced, and the use effect is better.

Provided is a method for producing, by culturing, a cell for measuring the activity of a sensor protein that can be used as a chemical substance sensor. A method for producing a cell for measuring the activity of a sensor protein, the method comprising: culturing a cell containing a polynucleotide containing a sensor protein coding sequence, and when the ratio (specific activity) of the activity value of the sensor protein when the concentration of a response substance is 1 [mu] M to the activity value when the concentration of the response substance is 0 [mu] M is 1.2 or more; and recovering the cells.

The invention relates to the field of cell culture. The invention particularly relates to culturing cells, such as fibroblasts, to obtain collagen sheets. Modified scaffolds have been found to improve the characteristics of the method. The invention also relates to a collagen sheet and to leather obtained by tanning such a collagen sheet.

The present invention provides an inbred corn line designated BGID2926, methods for producing a corn plant by crossing plants of the inbred line BGID2926 with plants of another corn plant. The invention further encompasses all parts of inbred corn line BGID2926, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line BGID2926, and plants produced according to these methods.

This invention relates to the field of cell biology, specifically to a co-culture cell model and method for primary glial cells and neurons. The method for co-culturing primary glial cells and neurons provided by this invention includes the following steps: a mixture of primary glial cells and neurons is seeded into a first co-culture medium for pre-culture; the medium is discarded and the cells are washed; a second co-culture medium is added for a first-stage culture; after the first-stage culture, a second-stage culture is performed; the medium used in the second-stage culture is a third co-culture medium supplemented with macrophage colony-stimulating factor; after the second-stage culture, a third-stage culture is performed; the medium used in the third-stage culture is the second co-culture medium; in the obtained co-culture cell model, microglia are differentiated and mature, and are in a quiescent state, thus obtaining a primary glial cell and neuron co-culture model.

A substrate for culturing cells that comprises a modified prolamin having a net positive charge is provided. The modified prolamin may be a prolamin that has been modified through amidation or esterification, such as the addition of a methyl-ester group or an ethanolamine group. The modified prolamin is animal-free may be food-grade and / or may be free of cross-linkers. The modified prolamin may be in a coating that can be applied to a surface for culturing adherent cells, such as a microcarrier (e.g., beads) or well plates. Substrates coated with the modified prolamin are able to support cell expansion and can be used for food-grade applications such as cultured meat.

The application discloses a preparation method of a layer-by-layer self-assembly tissue engineering membrane and application thereof, and belongs to the technical field of medical preparations. The glass slide is taken out after being placed in the SA / glycerin / gelatin solution; the glass slide is taken out after being immersed in the CS / NaCl / glycerin / gelatin solution; the glass slide is immersed in a CaCl2 solution, taken out, dried, and then immersed in ethanol; and the membrane taken off from the glass slide is dried and stored. The application uses chitosan and sodium alginate as main raw materials, and prepares a deformable tissue engineering membrane through a self-assembly and ion replacement method. 2+ and Na + alternating treatment to realize control deformation. The membrane in a diastolic state is used to culture cells until the cells on the membrane surface form a dense monolayer of cells, and then the monolayer of cells is separated from the lower self-assembly membrane through CaCl2 solution treatment, so that the monolayer of cells on the membrane is separated from the lower self-assembly membrane. Thus, a monolayer of cells for tissue engineering is obtained.

A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.

The present invention addresses the problem of simplifying the structure of a cell-culturing container (1) and suppressing the generation of a dead space. A cell-culturing container (1) is provided with a container body (2) having a bag part (20) and a culture chamber (21) which is partitioned inside the bag part (20) and in which a content liquid (A) containing cells and a culture solution for culturing cells is stored. The bag part (20) has: an expansion / contraction section (200) that has expansion / contraction properties and gas permeability over the entire part; and a port section (201) that is contiguous with the expansion / contraction section (200) and has an opening (201c) that is in communication with the culture chamber (21). The volume of the culture chamber (21) is variable in association with the expansion and contraction of the expansion / contraction section (200).

The application discloses a kind of mixed immune cells and its culture system and in vitro culture method.The mixed immune cells include 75%~80% of γδT cell, 10%~15% of NK cell and 1~2% of NKT cell.The in vitro culture method of the application includes, when peripheral blood mononuclear cell is inoculated with activation culture medium, adding immune cell first activation component, adding immune cell reactivation component after 0~6 hours of inoculation, continuous activation culture;Then the activated culture cell is transferred into the coated culture plate or culture bottle, and the mixed immune cell is obtained by expansion culture.The mixed immune cell of the application has higher liver cancer cell HepG2 killing effect.The mixed immune cell in vitro culture method of the application has high expansion efficiency, and the expansion multiple can reach 2000-8000 times.

The present invention provides an inbred corn line designated BGFA3005, methods for producing a corn plant by crossing plants of the inbred line BGFA3005 with plants of another corn plant. The invention further encompasses all parts of inbred corn line BGFA3005, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line BGFA3005, and plants produced according to these methods.