11 results about "Chemically defined medium" patented technology

Methods for generating mature somatostatin+ interneurons from human forebrain neural progenitor cells are provided using chemically-defined culture media in a two-stage culture protocol. The mature somatostatin+ interneurons are generated from medial ganglionic eminence neural progenitor cells (MGE-NPCs), which themselves are differentiated from pluripotent stem cells. Culture media, isolated cell populations and kits are also provided.

The present invention provides methods of differentiating pericytes from pluripotent stem cells comprising culturing steps in chemically defined culture medium. A population of exogenously derived pericytes from PSCs are also contemplated. Further uses of the exogenously cultured pericytes for an in vitro disease model or in vitro angiogenesis assay are contemplated, including an in vitro 3D model of vasculature.

A biologic composition for promoting wound healing includes: a human mesenchymal stem cell (MSC)-derived extracellular matrix (ECM) biologic component, and a negatively charged polymer, combined with the biologic component to form the biologic composition. A method of preparing the biologic composition thereof includes: (i) isolating human mesenchymal stem cells (MSC) and subjecting the MSC to hypoxia-priming in a xeno- and / or serum-free, or chemically defined medium to stimulate the production of an MSC-derived biologic component with pro-angiogenic factors; (ii) aggregating and co-precipitating the MSC-derived biologic component with a negatively charged polymer into a pericellular space of the MSC to produce an aggregated biologic component; (iii) assembling the aggregated biologic component and the negatively charged polymer into an insoluble extracellular matrix (ECM) by the MSC; and (iv) decellularizing the insoluble ECM to obtain the biologic composition comprising MicroParticles of Solidified Secretome (MIPSOS).

The invention provides methods for generating human CD34+, CD43+, and CD90+ hematopoietic progenitor cells from hematopoietic stem cells. The methods include the use of chemically-defined culture media. Isolated cell populations are also provided.

The invention provides methods for generating human CD34+, CD43+, and CD90+ hematopoietic progenitor cells from hematopoietic stem cells. The methods include the use of chemically-defined culture media. Isolated cell populations are also provided.

The application relates to the technical field of microbial culture medium, and discloses a chemical component limited culture medium for supporting growth of bifidobacterium and application thereof. The CDM comprises a carbon source, salts, amino acids, vitamins and water; the carbon source is selected from at least one of glucose, fructose, lactose, maltose and galactooligosaccharide, the salts comprise sodium acetate, potassium hydrogen phosphate, ammonium acetate, magnesium sulfate heptahydrate, ferrous sulfate heptahydrate, sodium chloride and manganese sulfate monohydrate, the amino acids comprise alanine, cystine, cysteine, tryptophan and asparagine, and the vitamins comprise pyridoxine hydrochloride, nicotinic acid, calcium pantothenate, thiamine hydrochloride, riboflavin, p-aminobenzoic acid, folic acid and biotin. The CDM of the application is a liquid culture medium, only comprises 21 definite components, is simple in components, convenient in preparation, and is high in universality, and can effectively support growth of bifidobacterium.

Methods for generating hepatocytes from induced pluripotent stem cells (iPSCs) are provided using chemically-defined culture media in a four-stage culture protocol. Culture media and isolated cell populations are also provided.

Methods for generating pre-oligodendrocyte progenitor cells (pre-OPCs) and oligodendrocyte progenitor cells (OPCs) from human pluripotent stem cells are provided using chemically-defined culture media that allow for generation of pre-OPCs and OPCs in as little as three days. Culture media, isolated cell populations and kits are also provided.

The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. TaXULne can be added to the serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.

Provided are a kit for inducing differentiation of stem cells into dopaminergic neural precursor cells and / or dopamine neurons, and a use thereof. The kit uses chemically defined culture media, chemical small molecules and cytokines, and does not involve substances such as animal-derived components which easily cause unstable factors such as batch-to-batch variation. Moreover, the differentiation period is shortened, and the kit can be used in clinical practice.

The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. TaXULne can be added to the serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.