116 results about "Bone morphogenetic protein" patented technology

The invention provides an application of bifidobacterium animalis subsp. Lactis CP-9 in preparation of a product for promoting bone growth and development or improving bone health. Long-term research and a large number of experiments show that the bifidobacterium animalis subsp. Lactis CP-9 can improve the bone volume fraction, the bone trabecula thickness, the bone density and the bone volume of an organism, and can also effectively improve the growth hormone level and the insulin-like growth factor 1 level, so that bone growth and development are promoted. Meanwhile, the bifidobacterium animalis subsp. Lactis CP-9 can effectively improve the N-terminal propeptide level, osteocalcin level, bone morphogenetic protein level and osteoprotectin level of I-type procollagen in a body, and remarkably reduce the amino-terminal peptide level of the I-type collagen and the ligand level of nuclear factor kappa B receptor activating factor, so that osteoblast differentiation is promoted, osteoclast differentiation is inhibited, and osteoclast differentiation is inhibited. Furthermore, bone formation is promoted, bone resorption is inhibited, bone metabolism is improved, and finally the effect of improving bone health is achieved.

The invention relates to the field of biomedical textile materials, and discloses a partially absorbable sports medicine orthopedic suture line and a preparation method thereof.The suture line is prepared from, by mass, 50%-90% of polylactic acid and 10%-20% of polyglycolic acid. The mass content of polyglycolic acid is 10%-50%, the composite material further comprises a bioactive component, the mass content of the bioactive component is 0.1%-10%, the bioactive component comprises bone morphogenetic protein, silver nanoparticles and hydroxyapatite, the tensile strength of the suture line is 30-120 MPa, and under different physiological conditions, the suture line is not prone to fracture, and the suture line is not prone to fracture. And the degradation product can be compatible with surrounding tissues. The shape of the suture line can be automatically adjusted at the physiological temperature by embedding the shape memory alloy, and the suture line can adapt to dynamic changes of the tissue and provide uniform tension and support when interacting with the biological tissue due to the intelligent response capability, so that the tissue healing is effectively promoted.

This application relates generally to the field of Bone Morphogenetic Protein (BMP) antagonists (soluble ALK6 fusion proteins), compositions thereof, and methods for use in treating neurodegenerative diseases and / or demyelinating diseases.

The invention discloses an active bone defect repair material and a preparation method thereof. The bone defect repair material is prepared by compounding collagen extracted from an animal source, a calcined bone matrix and bone morphogenetic protein composite microspheres and molding through a photocuring 3D printing technology. From the perspective of bionics, collagen is used as a framework material, a calcined bone matrix with low immunogenicity is used as a hard material to enhance mechanical support of the material, and bone morphogenetic protein composite microspheres have a synergistic effect, so that undifferentiated mesenchymal stem cells are guided to develop towards a specific direction in a long-acting manner. After the active bone defect repair material is implanted into a bone defect part, a microenvironment beneficial to cell adhesion and proliferation is provided for cells, and meanwhile, bone morphogenetic protein is slowly released and can act on the bone defect part for a long time, so that the differentiation and maturation process of osteoblasts is effectively promoted, and bone repair of the bone defect part is promoted.

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

The invention provides a DNA tetrahedron-based compound as well as a preparation method and application thereof, and belongs to the field of medicines. The invention designs a bioactive composite hydrogel scaffold modified by a functionalized DNA tetrahedron compound containing BMP-2, and the functionalized BMP-2 binding peptide is stably combined with a tetrahedron framework structure with a unique three-dimensional space structure and fine programmability in a chemical click mode. And further preparing with methacrylated hyaluronic acid to obtain the composite hydrogel stent. The functional DNA tetrahedron compound can efficiently capture BMP-2, the composite hydrogel scaffold can capture endogenous BMP-2 in a targeting manner in the early stage of bone defect and stimulate stem cell osteogenic differentiation so as to promote in-situ osteogenesis, a series of complications caused by delivery of exogenous bone morphogenetic protein-2 are avoided, the in-vivo stability is poor, and the in-vivo bone morphogenetic protein-2 can be used for preparing the bone morphogenetic engineering scaffold. The method has the advantages of high safety, high cost and the like, and has a good application prospect.

The present invention provides methods of generating bone and cartilage in vivo. The methods include delivering leukocyte cell-derived chemotaxin-1 (LECT-1) biomolecules and bone morphogenetic protein-2 (BMP-2) biomolecules into an in vivo target site, preferably by using biocompatible scaffolds containing the biomolecules.

The invention belongs to the technical field of stem cell culture, and discloses a culture method of bone marrow mesenchymal stem cells. A culture medium added with glutathione, L-ascorbic acid, L-glutamine, human serum albumin, sodium hydrosulfide, insulin, insulin-like growth factor-1, deer bone morphogenetic protein and antler stem cell exosome extract is used for culturing the bone marrow mesenchymal stem cells, and osteogenic differentiation of the bone marrow mesenchymal stem cells can be directionally induced. The culture medium does not contain animal serum, the formula is simple, the components are controllable, the cultured bone marrow mesenchymal stem cells are high in activity and good in state, the expression of marker genes of osteogenic differentiation is up-regulated, the regulation and control efficiency and accuracy of osteogenic differentiation are remarkably improved, and sufficient cell sources are provided for scientific research and clinic.

The present invention provides a pharmaceutical formulation, a pharmaceutical composition, a method for inducing new bone formation and a method for accelerating and enhancing spinal fusion and treating bone defects in a subject. The composition comprises autologous coagulum as the carrier for osteogenic bone morphogenetic protein BMP6, which has no immunogenicity to cause inflammation and ectopic bone formation and presents a higher potency for bone formation and reduction of drug usage.

The present invention relates to an inhibitor of R-spondin 2 and / or R-spondin 3 mediated bone morphogenetic protein (BMP) receptor inhibition for use in treating and / or preventing leukemia in a subject; and to methods, kits, combined preparations, and uses related thereto.

Disclosed herein are methods for treating a disease or disorder of the central or peripheral nervous system by administering to a subject in thereof an agent capable of modulating the activity or expression of bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI). Additionally, methods for screening agents capable of modulating the activity or expression of BAMBI are disclosed.

The invention relates to the technical field of biology, and discloses a culture medium system for pluripotency maintenance and directional induction of embryonic stem cells and application of the culture medium system. 0.5 to 0.8 part of tetramethylenediamine; 0.2 to 0.5 part of corpus luteum hormone; 1-2 parts of a vitamin mixture; 2-4 parts of bovine serum albumin; 0.5 to 1.0 part of sodium bicarbonate; 0.2 to 0.4 part of an antibiotic mixed solution; 1 to 2 parts of biotin; 0.5 to 1.0 part of bone morphogenetic protein; and 0.1 to 0.2 part of magnesium ethylene diamine tetraacetate. A multi-factor regulation and control system is constructed, signal inhibition, a three-dimensional scaffold and a metabolic regulation module are combined, pluripotency of the embryonic stem cells is stably maintained, culture uniformity, structural support and differentiation resistance are remarkably improved, and the technical bottleneck of a traditional system in the aspects of induction efficiency and stability is broken through.

To provide a method for producing a sheet-like cell culture showing a three-layer structure including a cell layer of superficial cells, a cell layer of intermediate cells, and a cell layer of basal cells, in this order. Provided is a method for producing a sheet-like cell culture that includes culturing a progenitor cell population in a second compartment separated from a first compartment via a porous member to form a sheet-like cell culture in the second compartment. The sheet-like cell culture includes a cell layer of superficial cells derived from the progenitor cell population, a cell layer of intermediate cells derived from the progenitor cell population, and a cell layer of basal cells derived from the progenitor cell population, in this order. The first compartment holds an induction medium containing retinoic acid, bone morphogenetic protein (BMP), and fibroblast growth factor (FGF).

The application provides a DNA tetrahedron-based complex and a preparation method and application thereof, and belongs to the field of medicines. The application designs a functionalized DNA tetrahedron complex modified bioactive composite hydrogel scaffold containing BMP-2, combines the functionalized BMP-2 binding peptide with the tetrahedron framework structure with unique three-dimensional spatial structure and fine programmability through a chemical clicking mode, and further prepares the composite hydrogel scaffold by combining with methacrylated hyaluronic acid. The functionalized DNA tetrahedron complex realizes the performance of efficiently capturing BMP-2, and the composite hydrogel scaffold realizes the targeted capture of endogenous BMP-2 in the early stage of bone defect and stimulates the osteogenic differentiation of stem cells to promote in-situ osteogenesis, avoids a series of complications caused by the delivery of exogenous bone morphogenetic protein-2, and solves the problems of poor in-vivo stability, safety and high cost, and has a good application prospect.

The present application belongs to the field of biomedicine, and relates to an inducer for inducing mesenchymal stem cells to differentiate into estradiol-secreting cells. The inducer for inducing mesenchymal stem cells to differentiate into estradiol-secreting cells is based on a human mesenchymal stem cell serum-free culture medium, and is composed of the following components in a mass concentration ratio: bone morphogenetic protein-4 20-60 mg / L, bone morphogenetic protein-7 20-60 mg / L, tretinoin 2-8 mg / L, resveratrol 2-8 mg / L, icariin 2-8 mg / L, benzamide 2-8 ug / L, chloroplatinic acid hexahydrate 2-8 ug / L, ethanolamine 2-8 ug / L, erythropoietin 2-10 ug / L, and vascular endothelial growth factor 2-10 ug / L. The inducer for inducing mesenchymal stem cells to differentiate into estradiol-secreting cells has high induction efficiency.

The present invention provides a method for producing neural crest cells from pluripotent stem cells, the method comprising: a step (1) of subjecting pluripotent stem cells to suspension culture in a culture medium containing an ALK inhibitor and a bone morphogenetic protein; and (2) performing suspension culture in a culture medium containing an ALK inhibitor and a GSK-3beta inhibitor.

The application provides a bone morphogenetic protein BMP-2 recombinant protein, and the amino acid sequence of the BMP-2 recombinant protein is shown as SEQ ID No. 1. The coding gene, the expression plasmid, the prokaryotic expression vector and the prokaryotic expression method are also disclosed. The prokaryotic expression system is adopted, and a solubilization tag is creatively introduced, so that the inclusion body expression is converted into soluble expression. The BMP-2 is expressed in the form of inclusion body in the prokaryotic system, the protein is converted into soluble expression by adding the solubilization tag, the difficulty of protein separation and purification is reduced, the purification cycle is shortened, and the purification efficiency is improved.

The invention relates to the field of bone tissue engineering and regenerative medicine, in particular to non-denatured bone protein calcium superfine powder with bone tissue oriented growth and rapid repair effects and a preparation method and application of the non-denatured bone protein calcium superfine powder, and the non-denatured bone protein calcium superfine powder comprises the following components in percentage by weight: 25%-45% of non-denatured type I collagen, 0.005%-0.05% of bone morphogenetic protein-2, 0.001%-0.02% of transforming growth factor-beta1, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium The bone tissue repairing agent is prepared from, by weight, 0.001%-0.02% of basic fibroblast growth factors, 20%-40% of ultramicro hydroxyapatite nanoparticles, 5%-15% of chitosan oligosaccharide, 3%-10% of mannitol calcium complex, 2%-8% of sodium hyaluronate, 0.01%-0.1% of vitamin D3, 0.01%-0.1% of vitamin K2, 5%-15% of chondroitin sulfate, 1%-4% of casein phosphopeptides and the balance auxiliary materials, and directional growth and rapid repairing of bone tissue are promoted.

The invention provides a bone repair material and a preparation method and application thereof, the bone repair material is composed of BMP protein (bone morphogenetic protein), carboxymethyl chitosan and calcium chloride, the carboxymethyl chitosan is crosslinked through calcium chloride to form nanoparticles, and the BMP protein is effectively loaded through electrostatic interaction; the bone repair material has good biocompatibility, biosafety and biodegradability, not only can effectively wrap and protect BMP protein from rapid degradation by enzyme, enhance in-vivo stability of BMP protein and realize continuous and controllable release of BMP protein, but also is beneficial to reduction of oedema and ectopic bone risks due to low-dose medication, and has good application prospects. And finally, by promoting angiogenesis, promoting bone tissue development and relieving inflammation, formation and repair of bones are achieved.

The invention relates to the technical field of oral medical materials, in particular to a tissue regeneration-guided oral repair membrane and a preparation method thereof. Comprising an outer layer and an inner layer, wherein the outer layer is a porous scaffold layer formed by a degradable polymer A, and the polymer A is selected from at least one of polylactic acid, polyglycolic acid, polycaprolactone or a copolymer thereof; the inner layer is loaded with a biocompatible hydrogel layer of a bioactive factor, and the bioactive factor is selected from bone morphogenetic protein, platelet-derived growth factor, vascular endothelial growth factor or a combination thereof. Comprising the following steps: preparing an outer layer solution; forming an outer-layer stent; preparing an inner layer precursor solution; gradient compounding and interface construction; performing crosslinking and curing; and post-processing. The invention provides a novel repairing film with intelligent degradation adaptability and precise biological factor controlled release capability.

The invention relates to the technical field of biomedical materials, in particular to an fCA-BExo composite material and a preparation method and application thereof. According to the complex, the cobalt-aluminum double hydroxide nanosheets are synthesized, mesenchymal stem cells are induced to secrete exosomes rich in bone morphogenetic proteins, fCA-BExo is formed, and the multiple effects of resisting oxidation, removing ROS, inhibiting inflammatory response and promoting osteogenic differentiation are achieved. The layered structure and surface electron vacancies of the nanosheet bring excellent ROS removal performance and biocompatibility, the exosome synergistically optimizes targeting and osteogenic induction effect, and the bone marrow microenvironment can be significantly improved. The complex can be efficiently dispersed in PBS or normal saline, is applied to osteoporosis diagnosis and treatment, and has the advantages of simplicity and convenience in operation, safety, high efficiency and transformation potential.

The application relates to the technical field of orthopedic repair material preparation, and discloses a sterile preparation and injection device for autologous red blood cell drug-loaded blood gel, which comprises a cross bolt mixer, a multimodal injector combined with a composite syringe and a mixing flow channel, the cross bolt is internally integrated with a microfluidic mixing channel, a vertical shaft direction and a coronal shaft direction are respectively connected with a main injector and a drug-loaded injector, when the red blood cell-rich plasma enters the bolt, longitudinal shear force is generated through a spiral flow channel, so that bone morphogenetic protein suspension is vertically injected into a main flow channel through a one-way valve structure, equal ratio mixing of 4:1 is realized in the spiral channel, and the whole mixing process is completed in a closed system. The device has the advantages of realizing accurate and uniform mixing of the red blood cell-rich plasma and the drug in a sterile environment, reducing the risk of bacterial contamination, being capable of directly injecting and filling bone defects for the prepared blood gel, being excellent in biological safety and compatibility, and being low in preparation cost and simple in process.

The invention provides a method of producing a population of CD34+ hematopoietic precursor cells. The CD34+ hematopoietic precursor cells are used in methods of producing natural killer (NK), methods of inducing NK cell differentiation from pluripotent stem cells (PSCs), and methods of generating terminally differentiated hematopoietic cells from PSCs. The differentiation of immune cells such as NK cells from PSCs includes the use of a hemogenic endothelium induction cocktail that includes a WNT signaling pathway activator, a bone morphogenetic protein and / or a vascular endothelial growth factor. Also provided is a method of producing hematopoietic stem cells from pluripotent stem cells.

The present invention discloses a method for substantially expanding the population of natural killer (NK) cells through the use of modified cellular culture conditions. By employing a targeted induction cocktail, including a WNT signaling pathway activator and bone morphogenetic protein (BMP), this technique facilitates the differentiation of pluripotent stem cells (PSCs) into NK cells. The core innovation lies in optimizing culture conditions, which significantly enhances the proliferation of NK cells. This approach streamlines the production of NK cells for therapeutic uses, emphasizing the expansion phase within the culture environment.

The present invention provides a method of producing a population of CD34 + hematopoietic precursor cells. Methods of using CD34 + hematopoietic precursor cells for producing natural killer cells (NKs), methods of inducing differentiation of NK cells from pluripotent stem cells (PSCs), and methods of producing terminally differentiated hematopoietic cells from PSCs. Differentiation of immune cells, such as NK cells, from PSC comprises the use of a hematopoietic endothelial induction mixture comprising a WNT signaling pathway activator, a bone morphogenetic protein, and / or a vascular endothelial growth factor. The invention also provides a method for producing hematopoietic stem cells from pluripotent stem cells.

The present invention provides an application of animal Bifidobacterium lactis subspecies CP-9 in the preparation of a product that promotes bone growth and development or improves bone health. After long-term research and a large number of experiments, the present invention found that animal Bifidobacterium lactis subspecies CP-9 can improve the body's bone volume fraction, trabecular thickness, bone density and bone mass, and can also effectively increase the level of growth hormone and insulin-like growth factor 1, thereby promoting bone growth and development. At the same time, animal Bifidobacterium lactis subspecies CP-9 can effectively increase the level of type I procollagen N-terminal propeptide, osteocalcin level, bone morphogenetic protein level, and osteoprotegerin level in the body, and significantly reduce the level of type I collagen amino-terminal peptide and nuclear factor κB receptor activator ligand level, thereby promoting osteoblast differentiation, inhibiting osteoclast differentiation, and then promoting bone formation, inhibiting bone resorption, improving bone metabolism, and ultimately achieving the effect of improving bone health.

Disclosed herein are methods of immunoassay for detecting, in a patient sample, the third laminin-like globular domain (LG3) fragment cleaved and released from endorepellin / perlecan by bone morphogenetic protein-1 (BMP-1). Also disclosed are monoclonal antibodies and immunoassay kits for use in these methods of immunoassay, as well as the use of these methods of immunoassay for detecting and / or monitoring fibrotic kidney diseases, such as Lupus Nephritis (LN) or IgA nephropathy (IgAN), or particular levels of severity thereof.

The present invention proposes a nanosphere drug delivery system for preventing tendon adhesion, and its preparation method and application, which belong to the field of medical technology. Exosomes are extracted after incubation and culture of bone marrow mesenchymal stem cells, embedded in mesoporous silica microspheres, modified by tannic acid and magnetic particles, adsorbed with 6-fructose disodium phosphate and bone morphogenetic protein composition, and the surface of the obtained microspheres is coated with hyaluronic acid-silk protein peptide to obtain a nanosphere drug delivery system for preventing tendon adhesion. The nanospheres prepared by the present invention have adjustable particle size, good dispersibility, uniform particle size, and good mechanical properties, biocompatibility and degradability. In terms of structure, it has a good hollow structure and a large specific surface area effect, can efficiently load drugs, promote targeted release and sustained-controlled release of drugs, and has a good effect on promoting healing and preventing and reducing tendon adhesion.

The invention discloses a porous microsphere system for bone regeneration and preparation and application thereof, and relates to the technical field of biomedical engineering and tissue engineering.The porous microsphere system is characterized in that a porous polylactic acid-glycolic acid copolymer microparticle library with adjustable pore diameters is prepared by combining a one-step emulsification method with a chemical etching process; by using the porous particles with multiple functions and drug-loading active compounds, the material can simultaneously bear the functions of an ECM simulation stent assembly and a self-assembly bone-like micro-tissue delivery carrier; in addition, the particle can also be used as a multifunctional carrier of various bone repair factors, including bone morphogenetic protein (BMP-2), nano-hydroxyapatite (nHA) and human umbilical vein endothelial cells (HUVECs). Precise regulation and control of the pore size of the microspheres are achieved through a one-step emulsification method and a chemical etching process, the microspheres can efficiently load BMP-2, nHA and HUVECs, multiple requirements of bone regeneration are met, and the formed three-dimensional micro-tissue has osteogenic differentiation and vascularization functions and is suitable for in-vitro models and clinical treatment.