The invention discloses a preparation method of a silver / fibroin / hydroxyapatite biomimetic coating-modified artificial ligament. The method concretely comprises the following steps: 1, carrying out surface modification on an artificial ligament material, carrying out plasma processing, taking out and exposing the material to air, feeding the material into a grafting agent solution, cleaning the material with pure water and drying the material in vacuum, so as to obtain the surface-modified artificial ligament material; and 2, adding a soluble silver salt to a fibroin solution, adding a calcium source, stirring the solution to form a mixed solution, feeding the surface-modified artificial ligament material to the mixed solution, fully stirring the solution evenly, irradiating under sunlight or artificial visible light, sucking up by a filter paper, putting into a Na2HPO4 solution, standing still, then taking out and sucking up; and repeating the cycle. Compared with the prior art, the preparation method disclosed by the invention has the advantages of the biocompatibility and the osteoconduction property of the artificial ligament material can be improved, and the artificial ligament has antibacterial and anti-inflammatory properties, and can meet the requirements of clinical medicine on the aspects of biological activity and mechanical property.