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Preparation method of artificial ligament modified by silver/silk protein/hydroxyapatite biomimetic coating

A hydroxyapatite and artificial ligament technology, applied in the field of biomedicine, can solve problems such as synovitis, infection, tendon-bone healing, etc., and achieve the effect of reducing synovitis, solving uneven coating, and improving tendon-bone healing.

Active Publication Date: 2016-05-04
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the defects in the prior art, the purpose of the present invention is to provide a silver / silk protein / hydroxyapatite biomimetic coating modification artificial ligament preparation method, which mainly solves the problem that the artificial ligament is exposed in clinical application as a knee cross. Postoperative synovitis, infection, tendon-bone healing problems with grafts in ligament reconstruction surgery

Method used

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  • Preparation method of artificial ligament modified by silver/silk protein/hydroxyapatite biomimetic coating
  • Preparation method of artificial ligament modified by silver/silk protein/hydroxyapatite biomimetic coating
  • Preparation method of artificial ligament modified by silver/silk protein/hydroxyapatite biomimetic coating

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 30gAgNO 3 Add to 200ml 30mM silk protein solution, adjust the pH value to 8, stir at 25°C for 10min, add 30gCaSO4 to this solution and stir for 20min to form a mixed solution; in the background vacuum 5×10 -2 Pa, power 200W will use H 2 After the artificial ligament material was treated with plasma for 10 minutes, it was soaked in 30 mg / ml acrylic acid monomer grafting solution for 30 minutes, and finally washed with pure water and vacuum-dried; the surface-modified artificial ligament after vacuum drying was soaked in the above-mentioned mixed solution, fully After stirring evenly, irradiate under sunlight or artificial visible light for 30 minutes, blot dry with filter paper, and finally soak in 5ml50mmol / L freshly prepared Na 2 HPO 4 The solution was stirred and reacted at 25°C for 45 minutes, taken out, and dried at room temperature. Repeat the above steps 20 times to obtain a new artificial ligament modified with hydroxyapatite-doped silver composite silk protei...

Embodiment 2

[0044] 30gAgC 2 h 3 o 2 Add to 200ml of 100mM recombinant silk protein solution, adjust the pH value to 7.5, stir at 25 degrees Celsius for 20min, add 30g of CaCO 3 and fully stirred for 30min to obtain a mixed solution; in the background vacuum 6×10 -2 Pa, power 300W will use N 2 Plasma treated the PET artificial ligament for 20 minutes, then soaked it in 10 mg / ml polyethylene glycol grafting solution for 50 minutes, and finally washed it with pure water and dried it in vacuum; soaked the surface-modified artificial ligament material after vacuum drying in the above mixed solution , fully stirred evenly, irradiated under sunlight or artificial visible light for 30min, blotted dry with filter paper, and finally soaked in 10ml40mmol / L freshly prepared Na 2 HPO 4 Solution, stirred and reacted at 25 degrees Celsius for 10 minutes, took it out, and dried at room temperature; repeated the above steps 30 times to obtain a new type of artificial ligament modified by hydroxyapati...

Embodiment 3

[0046] Add 30gAgCl to 200ml70mM natural spider silk protein solution, adjust the pH value to 7, stir at 25 degrees Celsius for 15min, add 30gCaHPO to the solution 4 and fully stirred for 45min to obtain a mixed solution; in the background vacuum 8×10 -2 Pa, power 400W will use Ar 2 Plasma treated the PET artificial ligament for 30 minutes, then put it into a 50 mg / ml insulin grafting solution and soaked it for 20 minutes, and finally washed it with pure water and dried it in vacuum; soaked the surface-modified artificial ligament after vacuum drying in the above mixed solution, fully After stirring evenly, irradiate under sunlight or artificial visible light for 45 minutes, blot dry with filter paper, and finally soak in 5ml30mmol / L freshly prepared Na 2 HPO 4 Solution, stirred and reacted at 25 degrees Celsius for 25 minutes, took it out, and dried at room temperature; repeated the above steps 25 times to obtain a new type of artificial ligament modified by hydroxyapatite-d...

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Abstract

The invention discloses a preparation method of a silver / fibroin / hydroxyapatite biomimetic coating-modified artificial ligament. The method concretely comprises the following steps: 1, carrying out surface modification on an artificial ligament material, carrying out plasma processing, taking out and exposing the material to air, feeding the material into a grafting agent solution, cleaning the material with pure water and drying the material in vacuum, so as to obtain the surface-modified artificial ligament material; and 2, adding a soluble silver salt to a fibroin solution, adding a calcium source, stirring the solution to form a mixed solution, feeding the surface-modified artificial ligament material to the mixed solution, fully stirring the solution evenly, irradiating under sunlight or artificial visible light, sucking up by a filter paper, putting into a Na2HPO4 solution, standing still, then taking out and sucking up; and repeating the cycle. Compared with the prior art, the preparation method disclosed by the invention has the advantages of the biocompatibility and the osteoconduction property of the artificial ligament material can be improved, and the artificial ligament has antibacterial and anti-inflammatory properties, and can meet the requirements of clinical medicine on the aspects of biological activity and mechanical property.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a method for preparing an artificial ligament modified by a silver / silk protein / hydroxyapatite bionic coating. Background technique [0002] Polyethylene terephthalate (PET) artificial ligament is used as the graft choice for knee cruciate ligament injury reconstruction. Although the short-term curative effect and safety after operation are relatively satisfactory, it still occurs due to histocompatibility problems. Complications such as synovitis and infection; and the inflammatory products in the synovial fluid will damage the intra-articular structure and have a negative impact on tendon-bone healing. These have become important factors leading to the failure of knee cruciate ligament reconstruction surgery. Therefore, it is particularly important to improve the biocompatibility of PET materials and reduce postoperative inflammation at the graft site caused by the material defects of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/34A61L27/32A61L27/30A61L27/50A61L27/54
Inventor 陈世益张鹏陈俊蒋佳吴子英陈天午万方
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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