1503results about How to "Mild conditions" patented technology

The invention discloses an injection filler and a preparation method and application thereof, the filler is polycaprolactone particles, the polycaprolactone particles are spherical particles, the particle size of the spherical particles is 20-100 [mu] m, and the polycaprolactone particles have rough surfaces or unsmooth surfaces. The polycaprolactone microspheres have rough surfaces or unsmooth surfaces, so that the contact area with cells is increased, the adhesion ability and residence time of the cells are improved, the affinity of the polycaprolactone microspheres is improved, and the growth of collagen can be stimulated and accelerated. In the preparation process, the dosage of each component is scientifically screened, and on the basis of controlling the viscosity of a water-phase solution, the spherical polycaprolactone particles which are regular, rough in surface and uniform in particle size are obtained in cooperation with a rapid membrane emulsification method.

The invention discloses one-step solvent-free methyl phenyl-terminated vinyl polysiloxane and silica gel, and the methyl phenyl-terminated vinyl polysiloxane is prepared by the following steps: adding octamethylcyclotetrasiloxane, octaphenylcyclotetrasiloxane and dimethyl-terminated vinyl silicone oil into a reaction container, and uniformly stirring; vacuumizing the reaction container, and introducing nitrogen to form an oxygen-free environment; heating the reaction container to 180-200 DEG C step by step, and adding a catalyst at 50-80 DEG C; the step-by-step heating comprises a first-stage pre-polymerization reaction at the temperature of 90 DEG C or below and a second-stage polymerization reaction at the temperature of 90 DEG C or above, and methyl phenyl vinyl-terminated polysiloxane is prepared. The high-temperature-resistant and high-pressure-resistant methyl phenyl silica gel is obtained by mixing methyl phenyl vinyl-terminated polysiloxane with an inhibitor, a cross-linking agent and a cross-linking catalyst, degassing, heating, cross-linking and curing, the performance requirements of high-power semiconductor devices on high-pressure resistance, high-temperature resistance and the like of packaging materials can be met, the product quality is stable, and the production efficiency is high.

The invention discloses a low-temperature sintering asymmetric porous silicon carbide ceramic membrane separation layer and a preparation method thereof, the preparation method comprises the following steps: a) preparation of a coating liquid: firstly dispersing a binder in a solution, adding silicon carbide particle powder, carrying out magnetic stirring dispersion for a period of time, then adding a dispersant, and stirring; then putting the stirred and mixed film coating liquid and the grinding balls into a ball milling tank for ball milling; and adjusting the pH value of the ball-milled silicon carbide coating liquid with ammonia water, and carrying out vacuum defoaming to finally obtain the high-stability and uniformly-dispersed silicon carbide coating liquid. Wherein the addition amount of the silicon carbide coarse particles is 0.5-50 wt% of the mass of silicon carbide; b) coating; c) drying; and d) sintering. The asymmetric porous silicon carbide ceramic membrane prepared by the method is low in sintering temperature, and the prepared separation layer membrane is controllable in pore size and uniform in pore size distribution; the method has excellent application prospects in the field of membrane separation.

The invention provides a fluorine-containing aromatic acid ligand modified zirconium metal oxide nano-cluster photoresist material as well as a preparation method and application thereof. The preparation method of the fluorine-containing aromatic acid ligand modified zirconium metal oxide nano-cluster photoresist material comprises the following steps: mixing a Zr6O4 (OH) 4 (MAA) 12 solution and a fluorine-containing aromatic acid ligand compound solution, and carrying out reaction, rotary evaporation and drying to obtain the fluorine-containing aromatic acid ligand modified zirconium metal oxide nano-cluster photoresist material. The preparation method is simple, cheap and easily available in raw materials, low in cost and suitable for industrial production. The fluorine-containing aromatic acid ligand modified zirconium metal oxide nano-cluster photoresist material can be suitable for ultraviolet lithography, electron beam lithography and extreme ultraviolet lithography, and the formed photoetched pattern has the advantages of low line edge roughness, high resolution and the like, and has wide application prospects.

The invention relates to the technical field of biology, in particular to application of N-terminal truncated 8-isopentenyl transferase in production of 8-isopentene kaempferol. The invention provides the N-terminal truncated 8-isopentenyl transferase, and the N-terminal truncated 8-isopentenyl transferase can be used for carrying out isopentenylation on kaempferol to generate 8-isopentene kaempferol. Specifically, the saccharomyces cerevisiae is used as a chassis, under the condition that kaempferol is exogenously added, through overexpression of 8-isopentenyl transferase, biosynthesis of 8-isopentenyl kaempferol in the saccharomyces cerevisiae is realized, and meanwhile, a foundation is laid for subsequent biosynthesis of icaritin. The invention provides a novel reference scheme for biosynthesis of flavonoid compounds.

The invention relates to the technical field of fiber product treatment, and discloses a low-energy-consumption rapid chemical separation method for polyester cotton fiber products. The method comprises the following steps: mixing and dissolving a hydrogen bond donor and a hydrogen bond acceptor to prepare a betaine-based eutectic solvent, and mixing the betaine-based eutectic solvent with alkali liquor to obtain a eutectic solvent / alkali composite system; immersing the polyester-cotton product in the deep eutectic solvent / alkali composite system for dissolution reaction, filtering after the reaction is finished, taking undissolved components in the polyester-cotton product, washing with water, and drying; according to the method, a betaine-based eutectic solvent / alkali composite system is adopted, polyester fiber components are dissolved in a mild condition within a short time, meanwhile, cotton fiber components are not damaged, the method has the advantages of being low in cost, environmentally friendly and the like, and rapid quantitative analysis of the polyester and cotton fiber components is achieved.

The invention relates to a preparation method of a calendula fermentation product. The preparation method comprises the step of fermenting calendula with saccharomycetes and lactobacillus in sequence. According to the invention, the calendula is subjected to secondary fermentation by adopting a method of firstly fermenting by using the saccharomycetes and then fermenting by using the lactobacillus, and the calendula is fermented through the synergistic effect of the two bacteria, so that the obtained calendula fermented product has the comprehensive effects of moisturizing, repairing and relieving.

The invention relates to the technical field of isoflavone biotransformation, in particular to a soybean isoflavone aglycone preparation method based on enzymolysis. Comprising the following steps: inoculating Kluyveromyces marxianus into a basic culture medium for shake cultivation; after culturing, centrifuging, washing and resuspending to obtain a bacterial suspension as a leavening agent, and inoculating the bacterial suspension to a bean dreg composite culture medium for shaking table fermentation for 48-96 hours; centrifuging the fermentation liquor at 0-4 DEG C, and collecting supernate to obtain crude beta-glucosidase; the crude enzyme liquid is sequentially subjected to ammonium sulfate salting-out, ion exchange chromatography and twice sephadex chromatography, and purified beta-glucosidase liquid is obtained; and carrying out enzymatic reaction on soy isoflavone by using the purified beta-glucosidase liquid to obtain the soy isoflavone aglycone. The method has the advantages that the bean dreg culture medium is utilized for fermentation to produce beta-glucosidase, and the combined soy isoflavone is subjected to enzymolysis, so that the utilization of the byproduct bean dregs is improved, the biotransformation of isoflavone is realized, and the obtained product has better activity.

The invention relates to a two-component polyurethane elastic structural adhesive suitable for bonding a plastic base material of an automobile spoiler. The two-component polyurethane elastic structural adhesive consists of a component A and a component B in a volume ratio of (0.8-1.2): 1, the component A comprises 10-30 parts by weight of liquid polybutadiene polyol; 10 to 30 parts by weight of polyester polyol; 1-10 parts by weight of modified castor oil polyol; 1-10 parts by weight of a chain extender; 20-40 parts by weight of a filler; 1-10 parts by weight of a molecular sieve; 1-5 parts by weight of white carbon black; 0.2 to 2 parts by weight of a tackifier; the component B comprises the following components in parts by weight: 20-40 parts of isocyanate; 20 to 40 parts by weight of liquid polybutadiene polyol; 10-20 parts by weight of a plasticizer; 20-40 parts by weight of a filler; 1-5 parts by weight of white carbon black; 0.2 to 2 parts by weight of an anti-aging agent; 0.2 to 2 parts by weight of a water absorbent; and 0.2 to 2 parts by weight of carbon black. The two-component polyurethane elastic structural adhesive provided by the invention has low hardness and high elongation at break, can realize high adhesive force when SMC / PP and other base materials are only subjected to surface flame treatment, has a bonding failure mode of cohesive failure, and is especially suitable for bonding of automobile spoiler plastic base materials.

The invention relates to a method for synthesizing tetrasubstituted-1, 3-allene dicarboxylic acid, and discloses a method for synthesizing tetrasubstituted-1, 3-allene dicarboxylic acid by taking 1, 3-enyne and iodide as raw materials, potassium formate as a formate source, DABCO as a hydrogen transfer catalyst, Ir (bpy) 2 (dtbpy) PF6 as a photocatalyst and cesium carbonate as alkali through the following steps: introducing CO2 at normal pressure; the allene dicarboxylic acid compound can be obtained at excellent to good yield under the irradiation of visible light at room temperature for 24 hours, the reaction condition is mild, the regioselectivity is good, the functional group tolerance is good, and many defects in the prior art are overcome.

The invention discloses a biomass photo-thermal interface evaporation material based on a nano TiO2-CNT photocatalytic thermal insulation layer, a preparation method of the biomass photo-thermal interface evaporation material and application of the biomass photo-thermal interface evaporation material in solar-driven interface evaporation, photo-thermal seawater desalination and sewage treatment. The preparation method comprises the following steps: S1, pulp pretreatment: softening pulp and removing saccharides and impurities; s2, preparing a carbonization layer; s3, a photocatalytic heat insulation coating is coated; S3.1, a photocatalytic heat insulation coating solution is prepared, wherein nanometer titania, carbon nanotubes, polyvinyl alcohol and lauryl sodium sulfate are taken and added into water, and a photocatalytic heat insulation layer solution is obtained; s3.2, a photocatalytic heat insulation coating is coated; and S4, pyrrole coating: uniformly coating the carbonized layer of the cactus pulp block with pyrrole, then uniformly coating the carbonized layer coated with pyrrole with the oxidant aqueous solution, and carrying out full polymerization reaction to obtain the biomass photo-thermal interface evaporation material Ppy / CTPS cactus.

The invention relates to the technical field of oil field fracturing fluid, in particular to a preparation method of a high-temperature-resistant and anti-shearing organic boron-zirconium cross-linking agent. According to the technical scheme, the method comprises the following steps: 1) weighing deionized water and an alcohol stabilizer, adding the weighed deionized water and alcohol stabilizer into a reaction container, adding inorganic zirconium salt into the reaction container, and stirring until the inorganic zirconium salt is completely dissolved; 2) adding an organic ligand, stirring to dissolve the organic ligand, and continuously stirring for 0.5 hour; (3) adding a complexing agent, heating to 50-70 DEG C, stirring and keeping the temperature for 11-15 hours to obtain an organic zirconium cross-linking agent; and 4) adding the organic zirconium cross-linking agent obtained in the step 3) and a boron compound into a reaction container according to a ratio, and stirring at room temperature until the organic zirconium cross-linking agent and the boron compound are completely dissolved so as to obtain the organic boron zirconium cross-linking agent, and the preparation method has the beneficial effects that the prepared cross-linking agent has excellent temperature resistance and shear resistance, and is simple and convenient to operate, mild in condition, wide in raw material source and low in price.

The invention discloses a synthesis method of EVOH, and relates to the technical field of EVOH synthesis. According to the technical scheme, the method comprises the following steps: S1, washing an adsorbent in washing liquor, soaking in a hydrochloric acid solution, oscillating, filtering, naturally cooling to normal temperature, and baking; s2, adding the mixture into an organic solvent, and then injecting ethylene; s3, mixing vinyl acetate and an initiator, mixing the mixture with an adsorbent for reaction, adding a terminator copper acetate to stop the reaction to obtain a mixed solution containing an ethylene-vinyl acetate copolymer, filtering the mixed solution to separate the adsorbent, and then performing desolvation, removal of redundant vinyl acetate and alcoholysis to obtain an EVOH crude product; and S4, carrying out water washing and vacuum drying on the EVOH crude product, extruding, pelletizing, and cleaning to obtain the EVOH. According to the invention, the adsorbent is adopted to adsorb ethylene, so that ethylene and vinyl acetate are fully and effectively contacted, the polymerization process is further controlled, the reaction pressure is reduced, the molecular weight distribution of the product EVOH is narrow, and the ethylene content is high.

The invention relates to the technical field of biocatalytic synthesis. The invention particularly relates to a glycosyl transferase mutant and a method for catalytically synthesizing rebaudioside M2 by using the glycosyl transferase mutant. The invention relates to a glycosyl transferase mutant, which is characterized in that the glycosyl transferase mutant is A1) or A2): A1) is a protein which is obtained by substitution of amino acid residues on an amino acid sequence as shown in SEQ ID NO.20, has more than 90% of identity with an original protein and has the same function with the original protein; a2) a fusion protein obtained by connecting a tag to the N end and / or C end of A1).

The invention provides a rare earth feed liquid defluorination recovery method, which comprises: (1) adjusting the pH value of a rare earth feed liquid by using an alkaline material, and mixing the pH-adjusted rare earth feed liquid with a rare earth compound to carry out a one-step reaction; and (2) carrying out one-step solid-liquid separation treatment on the material obtained by the one-step reaction in the step (1) to obtain impurity-removed slag and rare earth purified feed liquid, mixing the obtained impurity-removed slag with an alkaline solution to carry out two-step reaction, and carrying out two-step solid-liquid separation treatment on the material obtained by the two-step reaction to obtain a rare earth enriched product and fluorine-containing waste liquid. By means of the defluorination recovery method, fluorine ions in the rare earth feed liquid can be removed, meanwhile, obtained impurity removal residues can be recycled, and resources are saved.

The present invention discloses a method for preparing nano-diamonds based on graphene vertical sheets. The present invention utilizes a hot wire chemical vapor deposition system to grow graphene vertical sheets containing tantalum atoms, and transforms the graphene vertical sheets into nano-diamonds under relatively low pressure conditions. The method has the characteristics of mild conditions and low cost. The method has low requirements on equipment, simple process and easy operation. The method has important scientific significance and value for developing a new method for preparing nano-diamonds.

The invention belongs to the related technical field of electrocatalysts, and discloses a cross array electrocatalyst and a preparation method and application thereof.The preparation method comprises the following steps that a nickel net subjected to corrosion treatment is put into a corrosion solution to be subjected to heat preservation reaction for preset time, and then the cross array electrocatalyst is obtained, the electrocatalyst is provided with a nanosheet array structure which is mutually crossed and supported; wherein hydrochloric acid is used for carrying out corrosion treatment on the nickel net; the etchant solution is obtained by dissolving sodium thiosulfate pentahydrate, nickel chloride hexahydrate, ferrous chloride tetrahydrate and sodium orthovanadate in an ethylene glycol solution. The catalyst prepared by the invention has a nanosheet array structure which is mutually supported in a crossed manner, and the special structure enables the catalyst to construct an ion confinement space, so that adsorption and enrichment of reactants on the interface of the catalyst are facilitated, a local reactant-rich environment is constructed, and excellent electro-catalytic oxygen evolution activity is shown.

The invention relates to a preparation method of a spiral dislocation stacked imine covalent organic framework material. The preparation method comprises the steps of preparing an imine covalent oligomer and preparing the spiral dislocation stacked imine covalent organic framework material. The invention also provides the spiral dislocation accumulation imine covalent organic framework material obtained by the preparation method and application of the spiral dislocation accumulation imine covalent organic framework material in light absorption semiconductor materials. The preparation method provided by the invention is simple, convenient, universal and easy to implement, and the imine covalent organic framework material with the spiral dislocation stacking structure is obtained, is clear in material structure and good in crystallinity, is a novel topological structure, and has a good potential application prospect in the photoelectric field.

The invention provides the application of the non-dehydrogenation after hydrogen decrepitation treatment in the aspect of improving the residual magnetism of the neodymium-iron-boron magnet. The hydrogen content of the high-hydrogen-content coarse powder which is not dehydrogenated after the hydrogen decrepitation treatment is 1500-3500 ppm. According to the method, dehydrogenation treatment is not carried out especially in the hydrogen decrepitation treatment process, and the alloy coarse powder with high hydrogen content is obtained and used for improving the residual magnetism of the neodymium-iron-boron magnet. According to the preparation method of the high-remanence neodymium-iron-boron magnet, the production process is creatively designed and improved, the total amount of rare earth is reduced through formula design, the hydrogen content of coarse powder is increased, the coarse powder is sieved to remove impurities, an antioxidant is added into the coarse powder, and the measures of secondary high-temperature sintering and the like are adopted, so that the high-remanence neodymium-iron-boron magnet is obtained. And finally, the oxygen content of the magnet is reduced, namely, the beta non-ferromagnetic phase is reduced, and meanwhile, the density of the magnet is improved, so that the residual magnetism Br of the neodymium-iron-boron magnet is effectively improved.

The invention provides a composition for improving sleep quality as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: performing supercritical fluid extraction on lavender, violet, rose and agilawood to obtain extracted oil, performing solid enzymolysis, performing immobilized ionic liquid extraction, and performing desorption to obtain extracted oil, and uniformly mixing the extracted oil with the extracted oil and a penetration enhancer to obtain the composition for improving the sleep quality. The prepared extract oil and the extract oil are mixed, and the penetration enhancer is added, so that the prepared composition for improving the sleep quality enters a human body through skin absorption, achieves the effects of regulating qi activity of viscera and regulating yin and yang of the body, has the effects of soothing the nerves, helping sleep, prolonging the sleep time, improving the sleep effect and relieving nervous mood, and has a wide application prospect.

The invention discloses aggregation-induced emission glycyrrhizic acid nanoparticles as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing glycyrrhizic acid or a glycyrrhizic acid derivative solution, berberine or a berberine derivative solution and a tannic acid solution to obtain a composite solution, adjusting the pH value of the composite solution to be acidic, and stirring to obtain the aggregation-induced emission glycyrrhizic acid nanoparticles. The nanoparticles are high in biocompatibility, have aggregation-induced emission characteristics and pH fluorescence responsiveness, and still show fluorescence characteristics after being digested by gastrointestinal tracts; the fluorescent probe can be used for efficient cell imaging and gastrointestinal tract fluorescence tracing, and has potential practical application value in the fields of functional food, biological medicine and the like.

The invention relates to the field of extraction and purification of active substances of natural products, and discloses plant active ingredients in camphor tree seed kernels and a separation and purification method thereof.The method comprises the steps that fresh camphor tree seed kernels and water are mixed, wet-process superfine grinding is conducted through a colloid mill at the room temperature, centrifugal separation is conducted to obtain a middle-layer water phase, and the middle-layer water phase is statically or dynamically adsorbed through HJ-18 macroporous resin; and desorbing with 80% ethanol, collecting a desorption solution, carrying out rotary evaporation, and freeze-drying to obtain the water-soluble active component of the camphor tree seed kernel. The invention provides a new process for enriching and purifying the active components of the camphor tree seed kernels, the active components of the camphor tree seed kernels are enriched and purified by taking water as an extracting agent and combining with macroporous resin, the operation is simple and convenient, the enrichment efficiency is high, the use amount of organic solvents such as methanol and ethanol is reduced, and the process is safe and environment-friendly. In addition, compared with an ethanol extraction method, the method has the advantages that the camphor tree seed kernel protein is not denatured, and the quality of the camphor tree seed kernel protein is improved; according to the technical scheme, a new solution is provided for comprehensive utilization of camphor tree seed resources.

The invention provides a method for selective copper deposition in copper smelting waste acid, and relates to the technical field of hydrometallurgy. The method for selectively depositing copper in the copper smelting waste acid comprises the following steps: mixing the copper smelting waste acid and a sulfur-containing organic compound to carry out copper precipitation reaction, and carrying out solid-liquid separation to obtain copper-removed liquid and a copper deposition product; sulfur-containing functional groups in the sulfur-containing organic compound comprise one or more of sulfonic acid groups, sulfydryl and thioether bonds. The sulfur-containing functional group in the sulfur-containing organic compound reacts with the copper ions in the reaction system for selective precipitation, so that the copper ions in the waste acid can be captured and removed, and the method has the advantages of high copper selectivity, high copper precipitation rate, fast reaction and mild conditions, does not carry rhenium element, can greatly reduce the loss of rhenium, and simplifies the process flow.

The invention discloses a preparation method of a material capable of realizing tetracycline adsorption and visible light degradation, and belongs to the field of antibiotic wastewater treatment. According to the combined optimization method, three strategies of doping transition metal nickel on UIO-66, adjusting defects of a manufacturing frame by using monocarboxylic acid benzoic acid and compounding with a black phosphorus sheet layer to construct a heterojunction are implemented at the same time. The integrated nano material which has efficient adsorption performance on tetracycline and realizes photocatalytic removal under visible light is synthesized through a solvothermal method by orderly adding reactants, performing successive ultrasonic compounding and controlling the addition amount of the reactants. The prepared material has high adsorption capacity of 358mg g <-1 > on TC, and can remove more than 99% of the initial concentration of tetracycline hydrochloride after being irradiated and degraded by visible light.

The invention belongs to the technical field of sodium battery material synthesis, and discloses an in-situ coating method of a sodium ion battery positive electrode material. On the basis of an O3 type sodium ion battery layered oxide electrode material, an amorphous coating layer is constructed on the surface of an oxide through a low-pressure vapor deposition method. The invention provides a novel positive electrode material coating method, the coated material can stably circulate under the high voltage of 2-4.2 V, and the air stability is obviously improved. The sodium ion battery positive electrode material disclosed by the invention is low in raw material cost and environment-friendly, the coating method is simple, and the method has pioneering and universality and has a good application prospect.

The invention discloses a covalent lysosome targeting chimera based on a nucleic acid aptamer and application of the covalent lysosome targeting chimera in protein degradation and immunotherapy, and belongs to the technical field of biology. The invention designs a covalent lysosome targeting chimera with click chemical reaction activity, and the simple and efficient covalent lysosome targeting chimera is used for realizing efficient protein degradation at the levels of cells and living bodies. When the tool is used for targeted degradation of immunotherapy biomarkers, immunogenic apoptosis of tumor cells can be caused, so that killing of an immune system to tumors is more efficiently activated. Meanwhile, the covalent lysosome targeting chimera overcomes the problem of immune-related side effects confronted by existing antibodies and polysaccharide peptide drugs, and can effectively avoid the potential occurrence risk of the immune-related side effects while efficiently degrading the target protein.

The invention discloses a bifidobacterium pseudocatenum freeze-drying protective additive and application, and the bifidobacterium pseudocatenum freeze-drying protective additive is prepared from the following raw materials in percentage by weight: 2%-10% of dried skim milk, 5%-20% of cane sugar, 1%-6% of fructo-oligosaccharide, 0.5%-3% of sodium glutamate, 0.5%-2% of sodium ascorbate, 3%-15% of betaine and the balance of distilled water. The freeze-drying protective agent is simple in component composition, the survival rate of the bifidobacterium pseudocatenum in the freeze-drying process can be increased by using the freeze-drying protective agent, the freeze-drying survival rate of the bifidobacterium pseudocatenum reaches 76.3% or above, the stability of the freeze-drying preparation is guaranteed, and meanwhile the obtained freeze-drying preparation has the advantages of being easy to operate and the like, and has good application prospects. The preparation method of the freeze-dried preparation is simple and feasible, mild in condition, good in reproducibility, strong in controllability, suitable for large-scale production, and more convenient for clinical use and storage.

The invention provides an ultraviolet curing adhesive. The ultraviolet curing adhesive is prepared from the following raw materials in parts by weight: 30-60 parts of urethane acrylate; 30 to 50 parts by weight of a reactive diluent; 1 part by weight to 5 parts by weight of a photoinitiator; 5-20 parts by weight of a special structure oligomer; 1-6 parts by weight of a coupling agent; 0-3 parts by weight of an auxiliary agent; the oligomer with the special structure is selected from one or more of metal polyester acrylate, carboxyl-containing acrylate oligomer and acrylic phosphate. Compared with the prior art, the ultraviolet curing adhesive provided by the invention adopts components with specific contents, realizes better overall interaction, and is strong in product adhesive strength, good in universality and excellent in ultraviolet aging resistance.

The invention discloses a process method for preparing a fluopyram key intermediate. The key intermediate is [3-chloro-5-(trifluoromethyl) pyridine-2-yl] ({[2-(trifluoromethyl) benzoyl] amino} methyl) dimethyl malonate, and the process method comprises the following steps: in the presence of a solvent 1, carrying out a reaction on a compound 2-[3-chloro-5-(trifluoromethyl) pyridyl] dimethyl malonate potassium salt and a compound 1-D in a reaction container, and distilling and recovering the solvent 1 to obtain the intermediate compound. Wherein the solvent 1 is selected from acetic ester solvents with the boiling point range of 40-110 DEG C at normal temperature and normal pressure. The process method is simple and convenient, the total yield is high, the solvent is easy to recover, and the product purity is high; the intermediate does not need purification post-treatment, can be directly used for synthesis of fluopyram, can be amplified, and is beneficial to cost reduction, efficiency improvement and commercial mass production.

The invention provides a preparation method of a mineralized collagen scaffold material with a bionic structure, and belongs to the field of biomedical engineering.The preparation method comprises the steps that an amorphous calcium phosphate solution stabilized by polyacrylic acid is prepared, and the molar ratio of Ca to P in the amorphous calcium phosphate solution is 1.67: 1; preparing a collagen solution containing noradrenaline; carrying out isoelectric focusing treatment on the collagen solution under a constant voltage to form a dense membrane, and stretching the dense membrane to generate 120-200% deformation, so as to obtain a collagen membrane with a bionic structure; and soaking the collagen membrane in the amorphous calcium phosphate solution, and then performing freeze drying to obtain the noradrenaline-mineralized collagen scaffold material. The mineralized collagen scaffold material prepared by the method has excellent biocompatibility, osteogenesis inductivity and sufficient mechanical strength, can effectively promote bone tissue defect repair, and has a good application prospect in the field of bone tissue engineering. The invention further provides the mineralized collagen scaffold material with the bionic structure and application of the mineralized collagen scaffold material.