398results about How to "Rapid enrichment" patented technology

The invention relates to a multifunctional double-layer core-shell structure magnetic nano particle. In the invention, a magnetic nano particle with a particle size of 1-300 nm is used as a core and coated with a double-layer shell consisting of a SiO2 layer with a thickness of 1-200 nm and a hydrolyzed silane coupling agent layer is 1-100 nm thick and comprises one or more multifunctional groups; the particle size and the shell layer thickness can be controlled through regulating the volumes, the weight ratios and the reaction time of the magnetic core, a silicon dioxide precursor, a silane coupling agent and a catalyst in a preparation process; the total particle size of the nano particle can be as small as 5-50 nm and as large as 700-800 nm; the nano particle can have superparamagnetism, paramagnetism and ferromagnetism according to the change of the magnetic core particle size; and one or more bioactive molecules can be connected into the shell layer of the magnetic nano particle or to the surface of the shell layer through a chemical method or a physical method. The invention also provides a preparation method of the multifunctional double-layer core-shell structure magnetic nano particle and application thereof. The particle preparation method has the advantages of simplicity, moderate condition, low cost and easy realization of industrial production. The nano particle can obtain different functions through connecting different bioactive molecules and can be applied to the fields of protein enrichment, biological detection, separation and purification, targeted drug carriers, cell imaging and medical imaging.

The invention discloses a magnetic fluorescent kit for rapidly detecting microbes as well as a preparation method and a use method thereof. The kit comprises two components: 1) immunomagnetic microspheres specifically bound with the microbes to be detected; and 2) immunofluorescent microspheres specifically bound with the microbes to be detected. The preparation method comprises the following steps: (1) preparation of the immunomagnetic microspheres; and (2) preparation of the immunofluorescent microspheres. The use method comprises the following steps: (1) adding the sample to be detected, lyophilized powder of the immunomagnetic microspheres and the immunofluorescent microspheres to a buffer solution; (2) ensuring the surfaces of the identified microbes to have antigenic determinants simultaneously bound with the immunomagnetic microspheres and the immunofluorescent microspheres; (3) enriching the microbes bound with the immunomagnetic microspheres through magnetic separation of the immunomagnetic microspheres; and (4) qualitatively and quantitatively judging the microbes by measuring the fluorescence intensity of the immunofluorescent microspheres bound with the separated and enriched microbes. The kit, the preparation method and the use method have the advantages of rapidness, quantitative property and wide scope of application.

The invention discloses an isothermal amplification kit for detecting SARS-COV-2 and a primer probe set. The kit comprises (1) inactivated lysate; and (2) an isothermal amplification system: a reaction buffer solution Buffer A, magnesium acetate Buffer B, negative control, nuclease-free water, a primer probe set and RAA enzyme. The lysate is used for rapid inactivation and release of viral nucleicacid, an isothermal amplification technology is utilized for rapidly enriching and amplifying a target area, whether a specific amplified product exists is rapidly determined through combination of aprobe with modification groups and a product with biotin, by a lateral chromatography technology and by use of colloidal gold developing. The kit is simple to operate, professional extraction reagents and detection instruments are not required limitation of detection laboratories and professionals is freed, the kit can perform rapid detection in an instrument-free state outdoors, the whole process only needs 30 min, and reading is very convenient.

The invention discloses a biomass carbon-loaded nano zero-valent iron material as well as a preparation method and application thereof. The method concretely comprises the following steps: adding walnut shell biomass carbon into an FeCl3.6H2O solution, mixing for 20-30 hours at the room temperature, then dropwise adding an NaBH4 solution into the mixture under the protection of inert atmosphere, and continuously stirring for 0.5-1 hour after adding; finally, carrying out suction filtration, taking filter residue, washing and drying to obtain the biomass carbon-loaded nano zero-valent iron material. The preparation method is simple; in the material obtained by the method, zero-valent iron particles are small in particle sizes, even in distribution and can not easily agglomerate, so that the biomass carbon-loaded nano zero-valent iron material can be applied to repairing pollutants such as organic matters and heavy metal in underground water and soil.

The invention provides a new method for preparing high purity chlorogenic acid, comprising the steps: after the stock solution of the chlorogenic acid is processed by washing impurity and eluting through at least four levels of macroporous resin series connection adsorption column, and then the eluate is regenerated for continuous countercurrent extraction, so that the high purity chlorogenic acid is obtained. The method is applicable to light concentration stock solution of the chlorogenic acid, and can complete the process of absorbing, washing impurity, eluting, regenerating, countercurrent extracting and recycling the solvent in a semicontinuous way; the handling capacity of samples is large, the operation is simple, the production cost can be lowered, the residual organic solvent is reduced, and the environmental pollution is relieved.

A zero-valent iron and biochar composite chemical for in-situ remediation of groundwater contaminated with chlorinated hydrocarbons is prepared from materials in percentage by weight as follows: 5%-30% of zero-valent iron powder, 8%-30% of a biochar source, 35%-85% of water, 0.1%-1% of a stabilizer, 1.5%-3% of a thickener, 0.3%-1% of an emulsifier and 0.01%-0.1% of a micronutrient source. The required raw materials are uniformly mixed by mixing equipment with high shearing for 15-30 min, and composite slurry which has good fluidity, is conducive to in-situ injection and can be stably stored is obtained. The prepared zero-valent iron and biochar source composite chemical has excellent fluidity and migration and diffusion capabilities and has a good remediation effect on complex contaminated sites containing both unsaturated and saturated chlorinated hydrocarbons. Besides, the selected raw materials are economic and available, the cost of the chemical is greatly reduced, and large-scale remediation of actual sites is facilitated.

The invention discloses a magnetic covalent organic framework nanocomposite material and a preparation method and application. The method comprises the steps that by means of a solvothermal reaction method, ferroferric oxide nanoparticles, 1,3,5-tris(4-aminophenyl)benzene and terephthalaldehyde serve as the raw materials, and the magnetic covalent organic framework nanocomposite material capable of being used for separating and enriching peptide fragments with benzene ring structures or hydrophobic peptide fragments is prepared under the catalytic action of acetic acid. The preparation process is simple and effective, reagent consumption is low, and the yield is high; the obtained magnetic covalent organic framework nanocomposite material not only has the advantages of covalent organic framework materials of being porous, stable in structure, large in specific surface area and the like, but also has the advantages of magnetic materials of being good in dispersity, easy to separate, good in magnetic inductivity and the like, and the magnetic covalent organic framework nanocomposite material has a good application prospect in the fields of proteomics, peptideomics and the like.

The invention discloses an SERS (surface-enhanced Raman spectrum) detection method for narcotics in a urine sample. The method comprises steps as follows: synthesizing a sol ultrafine grained nano-material with SERS active noble metals; dropping the sol ultrafine grained nano-material onto a substrate, and obtaining an SERS base through drying; taking the urine sample, sequentially adding a reagent I, namely, an alkaline solution, and a reagent II, namely, solid salt, to the urine sample, adding a reagent III, namely, an organic solvent, after even mixing, and then performing vibrating extraction on the mixture; taking an organic-layer solution after centrifugation or filtration by the aid of an organic filtration membrane; dropping the organic-layer solution onto the SERS base, and performing SERS detection on the organic-layer solution by the aid of a Raman spectrometer. According to the method, the urine sample of a person is pretreated, and the common narcotics in the urine sample are quickly separated, purified and enriched, so that the detection time is shortened, only 3-5 min is taken for the whole detection, and the detection sensitivity and the detection efficiency can be improved.

The invention provides construction of a surface plasma resonance sensor based on the amplification effect of a magnetic molecularly imprinted polymer and the application of the surface plasma resonance sensor in pesticide detection, and belongs to the technical field of surface plasma resonance sensing. Chlorpyrifos is regarded as a template molecule firstly; the magnetic molecularly imprinted polymer which has recognition action on the chlorpyrifos is compounded by adopting the self-polymerization performance of dopamine; after elution is performed on the chlorpyrifos, the magnetic molecularly imprinted polymer containing imprinted cavities has good superparamagnetism and high selectivity; the specific interaction between acetylcholinesterase fixed on the surface of a gold sheet and the chlorpyrifos is adopted to construct a pesticide residue recognition, detection and sensing interface on the basis of the amplification effect of the magnetic molecularly imprinted polymer. Compared with the conventional pesticide residue detection technology, the surface plasma resonance sensor on the basis of the amplification effect of the magnetic molecularly imprinted polymer has high detection sensitivity and good selectivity to chlorpyrifos detection, and has an excellent application prospect.

The invention discloses a preparation method of a high-performance gold-shell magnetic microsphere and surface enhanced Raman scattering (SERS) application of the high-performance gold-shell magnetic microsphere. A substrate uses a Fe3O4 microsphere of 100-1,000 nanometers as a core to provide magnetism, polymer polyethyleneimine (PEI) is used as a multi-functional sandwiched layer to improve the dispersibility of the magnetic microsphere and absorb gold seeds of 3-5 nanometers, and finally, a uniform and stable gold shell is prepared by a seed reduction method. The preparation method comprises the following steps of firstly, modifying Fe3O4 microsphere with the PEI to form a polymer protection layer, and absorbing the gold seeds of 3-5 nanometers by means of strong positive electricity of polyethyleneimine; secondly, rapidly reducing the uniform and stable gold shell under an ultrasonic condition by a seed growing method and by taking hydroxylamine hydrochloride as a reducing agent; thirdly, adding high-concentration povidone (PVP) to improve the dispersibility of particles; and finally, obtaining the high-performance gold-shell magnetic microsphere. The invention also discloses application of the gold-shell magnetic microsphere prepared by the method in SERS detection. The gold-shell magnetic microsphere is high in magnetic response, good in dispersibility and stable in structure, has excellent SERS performance and can be used for SERS detection and analysis on various pollutants, pesticide residue, pathogenic microorganism and the like in a solution.

The invention provides a composition and a kit for rapid and efficient extraction of a circulating unrelated nucleated cell from peripheral blood. The composition for rapid extraction of the circulating unrelated nucleated rare cell from the blood comprises buffer solution, hypotonic erythrocyte breaking solution, magnetic or non-magnetic immune micro-spheres coated with an anti-blood nucleated cell surface marker antibody and a cell separation medium with a certain density. Compared with the common commercial centrifugation medium on the market at present, the composition of the invention can greatly reduce the centrifugation time, and stably improve the cell recovery rate from original 60 plus or minus 20 percent to above 95 percent. When combined with the agent to remove leukocytes andhypotonic erythrocyte breaking means, the composition can be used for effectively and rapidly concentrating and extracting circulating unrelated nucleated rare cells in the peripheral blood, such as epithelial tumor cells and vascular endothelial cells. Therefore, the invention has great practical clinical significance for early diagnosis of tumors and heart diseases.

The invention relates to a beneficiation treatment method for electrolytic aluminium carbon residues. The method comprises the following steps: taking carbon residues out of the electrolytic cell for electrolytic aluminium, crushing and grinding the carbon residues into 20-60-mesh powder; adding water, a collecting agent and a foaming agent into the ground carbon residue particles to obtain ore pulp; and sequentially feeding the ore pulp into a roughing flotation machine and two scavenging flotation machines for flotation, wherein a foam product scraped by the roughing flotation machine is carbon powder and material obtained after the second scavenging flotation machine scavenges and scrapes off the foam product is filtered, dried and calcined to obtain a cryolite product. The beneficiation treatment method for electrolytic aluminium carbon residues can be used for enriching and efficiently recycling carbon and cryolite in electrolytic aluminium carbon residues.

The invention discloses a split-type nanometer fibre solid-phase extraction column and an application thereof, comprising a sample storage tube (1), wherein a fibre filling tube (2) is sleeved at the lower end of the sample storage tube (1) and nanometer fibre used as a column filler is filled inside the fibre filling tube (2). The extraction column has the advantages that the operation flow is simplified, the enriching efficiency is improved, the operation time is shortened, and the like. Compared with the traditional solid-phase extraction column, the utility model ensures that the use amount of adsorbent is greatly reduced and is convenient and rapid to manufacture and use.

The invention belongs to the technical field of bioanalysis and detection, in particular to a microfluidic chip for enriching microorganisms in air and a preparation method thereof. The chip uses optically transparent polydimethylsiloxane, and the like as materials by adopting a molding method, and the chip internally contains a sample enriching channel and a gas channel to realize the collectionof biologic grains of bacteria, fungi, viruses, and the like in an air sample. An enriched sample can be combined with a PCR (polymerase chain reaction) microfluidic chip and an immune microfluidic chip to carry out gene analysis or immunoassays so as to achieve the quick detection of the contents and the species of the microorganisms in the air. The invention has the characteristics of high speed, high efficiency, convenient taking, low price and easy automation control, and the integration of the microfluidic chip with other chips can complete automatic signal acquisition, remote transmission and signal analysis, thus the microfluidic chip can be used for the field quick detection and remote control detection within a large range on microorganisms in the air or other gases.

The invention discloses a method for enriching the specificity of a circulating tumor cell. The method comprises the following steps of: (a) preparing a primary antibody and a secondary antibody of a surface receptor of a tumor cell according to the requirement; (b) fixing the secondary antibody of the surface receptor of the tumor cell; (c) incubating the primary antibody and a sample to be detected; and (d) capturing the circulating tumor cell in the sample to be detected. The method disclosed by the invention is simple in operation; the secondary antibody is fixed and the incubated circulating tumor cell is captured through the fixed secondary antibody; the enrichment is conveniently and quickly realized and the operation process only takes 45 minutes; quick detection can be carried out after enrichment; and the method can be applied to the gene mutation detection of the circulating tumor cell in liquid by enriching.

The present invention relates to a method for quickly enriching trace polypeptide or protein sample by using nano calcium carbonate particles in-situ polymerized polymethyl methacrylate (CaCo3-PMMA) as nano adsorbing agent and utilizing solid-free miniaturated matrix auxiliary laser analysis ion source mass spectrography to make direct analysis. The invented nano material not only can implement quick and high-effective enrichment of protein and polypeptide, but also has good compatibility with matrix auxiliary laser analysis ion source mass spectrography. Said invention can be extensively used in the field related to protein phylogeny.

The invention provides a rapid and cheap method for producing compound magnetic flocculating agent and a method for fathering alga. The compound magnetic flocculating agent is composed of the reaction products of fly ash, hyperpure magnetite powder and concentrated hydrochloric acid and polymer chloridized aluminum and iron, which is capable of effectively absorb flocculating alga. The produced flocculating body is big and compact and precipitates rapidly. The alga flocculating body can be removed out of the water quickly via the foreign field, which can also apparently remove N and P from the water and solves the hazards from alga completely. The invention has extension and application foreground in the field of sea red current and lake eutrophication etc.

The invention discloses a method for quickly starting a shortcut nitrification reactor. The method comprises the following steps: after performing inoculation on the reactor, firstly performing overaeration, and applying an aeration impact so as to accelerate the elimination of anaerobic bacteria in sludge; quickly enriching nitrifying bacteria, and then applying a combined load impact comprising a substrate concentration impact and a hydraulic retention time impact so as to quickly restrain and eliminate nitrite oxidizing bacteria; finally, under the condition that an appropriate substrate concentration and an appropriate hydraulic retention time are maintained, increasing the concentration of an inorganic carbon source so as to enrich ammonia oxidizing bacteria and accelerate the accumulation of nitrite. According to the invention, the various continuous impacts are combined, the enrichment of the ammonia oxidizing bacteria in the sludge is accelerated within a shorter time, and the quick start of the shortcut nitrification reactor is realized.