30 results about "Nucleated cell" patented technology

The application provides a method for detecting HES1 markers of melanoma CTCs by using multiplex immunofluorescence technology, and the method comprises the following steps: S1, collecting peripheral blood of a melanoma patient, and performing sorting and enrichment of CTCs through a microfluidic chip; S2, fixing and permeating after making all nucleated cells in the blood flatly and enriched on an adherent slide through a centrifuge; and sequentially performing co-incubation of HES1, HMB45 and CD45 antibodies on the screened melanoma CTCs through the multiplex immunofluorescence technology, and further analyzing the expression of the HES1 antibody and performing image analysis and quantification. The method is simple in operation, high in detection accuracy, can avoid false negatives and the like, and improves the screening accuracy of melanoma CTCs.

The utility model discloses a rapid mononuclear cell separation tube capable of being easily filled with a partition plate, and belongs to the technical field of cell separation, the rapid mononuclear cell separation tube comprises a centrifugal tube and the partition plate provided with three supporting legs, the partition plate is provided with a peripheral through hole, and the side wall of the bottom in the centrifugal tube is provided with a flange surrounding the axis of the centrifugal tube; a supporting cover is arranged at the open end of the centrifugal tube, a supporting rod moving in the axis direction of the centrifugal tube is arranged on the supporting cover, and a tray used for supporting the partition plate and driving the partition plate to move relative to the centrifugal tube in parallel is arranged at the end, located in the centrifugal tube, of the supporting rod. The tray is provided with a protrusion matched with the peripheral through hole, the protrusion is provided with an air hole which is the same as the peripheral through hole and penetrates through the tray, and the device has the advantages that the partition plate can be rapidly and accurately filled under the condition that it is guaranteed that the partition plate is relatively parallel to the inner diameter of the centrifugal tube, the filling efficiency is greatly improved, and the partition plate and the centrifugal tube are firmly fixed.

The utility model relates to the field of centrifugal tubes, in particular to a centrifugal tube for separating nucleated cells and a matched adapter set. The centrifugal tube comprises a centrifugal tube body and an adapter arranged in the centrifugal tube body. The adapter comprises a contracted pipe piece, a positioning piece and an abutting piece. The pipe diameter of the centrifugal pipe can be reduced through the through hole formed in the pipe reducing piece, so that the nucleated cell layer is thickened. The positioning piece comprises a positioning block, a sliding plate and a connecting plate; and by pushing the sliding plate, the connecting plates at the two ends are clamped to the upper ends and the lower ends of the two positioning blocks correspondingly, so that the contracted pipe fitting is positioned. According to the utility model, the column body is adjusted between the two vertically adjacent positioning blocks, and the sliding plates are respectively pushed towards two sides, so that the connecting plates at two ends of the sliding plates are respectively clamped at the upper ends and the lower ends of the two positioning blocks, and the positioning of the column body is completed. After centrifugation is completed, a nucleated cell layer appears in the through hole, the inner diameter of the centrifugal tube can be reduced, and the nucleated cell layer is thickened, so that the nucleated cell layer is conveniently and accurately sucked out.

The invention discloses an immunofluorescence staining method for karyocytes in blood. When immunofluorescence staining is carried out, polylysine is used for treating a cell slide, and then the cell is fixed and smeared and dried, so that the cell and the slide are attached more tightly. The problem that cells fall off in the cleaning process can be effectively avoided. Meanwhile, non-blood cells in the blood sample can be effectively reserved.

The utility model relates to a cord blood hematopoietic stem cell collection, preparation and cryopreservation integrated quadruple bag which comprises a blood collection bag, a preparation bag, a plasma bag and a cryopreservation bag, and the blood collection bag is connected with the preparation bag through a first catheter and a first three-way pipe; a first flow stopping clip is arranged on the second catheter connected between the preparation bag and the first three-way pipe; the first three-way pipe is respectively connected with the plasma bag and the cryopreservation bag through a second conduit and a second three-way pipe; a second flow stopping clip is arranged on the second catheter; a third flow stopping clip is arranged on the fourth catheter connected between the plasma bag and the second three-way pipe; and a fourth flow stopping clip is arranged on the fifth catheter connected between the second three-way pipe and the cryopreservation bag. The quadruple bag is more convenient to extract cells, the cost is reduced, the consumed time is less, the recovery rate of nucleated cells is high, and the separation effect is better.

The disclosure provides methods for detecting circular endothelial cells (CECs) in a non-enriched blood sample. The present disclosure is based, in part, on the unexpected discovery that CECs can be detected in non-enriched blood samples. The present disclosure is further based, in part, on the discovery that CECs can be detected in non-enriched blood samples by combining the detection of one or more immunofluorescent markers in the nucleated cells of a non-enriched blood sample with an assessment of the morphology of the nucleated cells. The present disclosure is further based, in part, on the discovery that CECs can be detected in non-enriched blood samples by comparing the immunofluorescent marker staining and morphological characteristics of CECs with the immunofluorescent marker staining and morphological characteristics of WBCs. The methods disclosed herein serve to classify human subject in myocardial infarction (MI) patients or healthy controls.

This invention provides a means to obtain more clinically useful information when analyzing particles contained in blood samples using metachromatic positive dyes. In a particle analysis method for analyzing particles contained in blood samples, particles are stained with a metachromatic positive dye; the stained particles are irradiated with light; the intensity of a first fluorescence from the stacking component of the metachromatic positive dye and the intensity of a second fluorescence from the embedded component of the metachromatic positive dye are measured, the first and second fluorescence being emitted by each particle contained in the blood sample; the intensities of the first and second fluorescence emitted by each particle are normalized according to the size of each particle to obtain the fluorescence concentration of each of the first and second fluorescence in each particle; in a two-dimensional plot of the normalized fluorescence concentrations, each particle is clustered into multiple particle clusters, including at least two of erythrocyte clusters, platelet clusters, and nucleated cell clusters; and a histogram is created where the intensity of the first fluorescence is a histogram of the RNA amount of the class and the intensity of the second fluorescence is a histogram of the DNA amount of the class included in at least one of the multiple particle clusters.

A method for obtaining enucleated cells from nucleated cells is described herein. A cell processing method is also described herein, which includes providing a composition containing nucleated cells and enucleating at least a portion of the nucleated cells to produce an enucleated cell fraction.

Described herein are methods for obtaining enucleated cells from nucleated cells. Also described herein are methods for cell processing, including providing a composition containing nucleated cells and enucleating at least a portion of the nucleated cells to produce an enucleated cell fraction. Also described herein are methods for cell processing, including expressing the heterologous gene product. Also provided are pharmaceuticals compositions comprising an enucleated cell.

The application discloses a blood separation method and a separation device, relates to the field of blood separation, and needs to use a blood separation device, wherein the blood separation device comprises a centrifugal device and a separation tube; the separation tube is in circular motion with the rotation center of the centrifugal device as the center during use; the separation tube comprises a blood inlet, a plasma outlet, a red blood cell outlet and a nucleated cell outlet; the plasma outlet is located at one end close to the rotation center; the red blood cell outlet and the nucleated cell outlet are located at one end away from the rotation center; blood is continuously injected into the blood inlet after the movement of the separation tube, and the plasma, the red blood cells and the nucleated cells in the separation tube continuously or intermittently flow out when the volumes of the plasma, the red blood cells and the nucleated cells reach a separation threshold value during the period. Compared with the prior art, the application adopts a continuous centrifugal method to separate various components in blood by guaranteeing a centrifugal sedimentation distance, so that the separation effect is obviously improved, and the actual effect is very remarkable.

The present disclosure is directed to the stabilization of nucleated cells in a whole blood sample ex-vivo, effected by an additive being a liquid composition for stabilizing an analyte in intact nucleated cells of a whole-blood sample ex-vivo, the composition being an aqueous solution, the solution comprising an anticoagulant, a phosphate salt, a cell-metabolizable sugar, adenine, and an antioxidant, wherein the antioxidant comprises a mitochondria-targeted antioxidant, preferably a mitochondria-targeted antioxidant selected from the group consisting of SkQ1, MitoQ, SS-31, and a mixture thereof. In a specific embodiment, the liquid composition further comprises a protease inhibitor. Further provided is advantageous use of the composition for stabilizing an analyte selected from DNA, RNA and protein in intact nucleated cells of the whole-blood sample ex-vivo, as well as kits including the composition for practicing said use. Additionally, specific methods are provided for stabilizing intact nucleated cells of a whole-blood sample ex-vivo.

The utility model discloses a mononuclear cell separation bag, the open end of a whole blood sample bag is provided with a liquid one-way flow conduit and is connected with a blood sample transfer conduit, a plasma transfer conduit and a blood cell transfer conduit, the whole blood sample bag is connected with a plasma bag through the plasma transfer conduit, the open end of the plasma bag is provided with the liquid one-way flow conduit, and the blood cell transfer conduit is connected with the blood cell transfer conduit. The blood sample transfer catheter is connected with the plasma transfer catheter and the blood cell transfer catheter, the other end of the blood cell transfer catheter is connected with the blood cell transfer catheter, the other end of the blood cell transfer catheter is connected with the blood cell transfer bag, the open end of the blood cell transfer bag is provided with a sample extraction port, and the blood sample transfer catheter, the plasma transfer catheter and the blood cell transfer catheter are provided with disposable pipeline plugging devices and pipeline temporary sealing devices. The disposable closed container is used, so that the centrifugal tube is prevented from being frequently replaced, and meanwhile, the experiment failure caused by sample pollution due to the fact that hands of operators or articles pass through the upper part of the open centrifugal tube is also avoided.

The present invention provides a method for cell analysis, comprising: preparing a blood sample comprising nucleated cells having surface, cytoplasmic or nuclear antigens (markers); antibody staining the cell markers; fixing and permeabilising the cells; FISH probe hybridising to chromosomes in the cells; performing imaging flow cytometry on the cells; analysing data obtained from performing imaging flow cytometry; and diagnosing, prognosing or monitoring a medical condition based on the data analysis.

The utility model discloses a kind of nucleated cell kit canning device, it is related to reagent kit packaging technical field.The utility model includes support frame, further include: support part, the support part is set on support part;Filling part, the filling part is set on the top of support part;And blanking part, the blanking part is set on support part;Wherein, the bottom of support frame is equipped with motor slot, and the structure on the filling part is compatible with this motor slot.The utility model sets up filling part, when using, operating personnel first places the reagent kit to be filled in on feed conveyor belt, feed conveyor belt is sequentially conveyed to equidistant turntable according to pre-set program, at this time, motor one starts, and equidistant turntable is rotated by rotating shaft one drive, to avoid interference, feed conveyor belt pauses conveying during equidistant turntable operation, ensure that equidistant turntable can move reagent kit to the just below of adjusting plate on feed hopper.

A kind of children's leukemia multi-instance classification method based on hierarchical attention mechanism, first, carry out cell segmentation task: the nucleated cell in the multiple bone marrow cell images of children's leukemia patient is extracted by cell segmentation module, and the random sampling of N cells is formed into a multi-instance package as sample input learning;Second, carry out children's leukemia classification task: the above input sample after learning is used as children's leukemia classification module, distinguish each sample belongs to acute myeloid leukemia, or acute lymphoid leukemia, and further carry out multiple hierarchical typing;The method of the present application uses multi-instance learning, encodes cells into features, and calculates the contribution of different cells to each category through the attention module, and forms hierarchical features by typing, from the attention matrix of subtyping to the coarse typing, for the loss function of cancer cell proportion, by limiting the proportion, semi-supervisedly classifies whether each cell belongs to cancer cell, so that the attention mechanism can better calculate the contribution of cells to each category, so that the classification is more accurate.

The invention relates to the technical field of sample preservation. The invention provides an exfoliated cell preserving fluid and a preparation method and application thereof, the preserving fluid comprises a cracking agent, a buffering agent, a chelating agent, a mucus dissolving agent and a preservative, the cracking agent and the buffering agent comprise ammonium salt, and the ammonium salt comprises at least one selected from the group consisting of ammonium chloride, ammonium acetate and ammonium bicarbonate. The preserving fluid can selectively lyse red blood cells to reduce blood background interference, and strongly disperse and dissolve mucus to effectively release wrapped target cells; the morphological integrity and nucleic acid integrity of nucleated cells are protected, and morphological abnormalities such as solid shrinkage, dissolution or expansion of the cells are avoided. The biological stability of the sample in the long-term storage and transportation process is ensured due to strong anti-corrosion and antibacterial capabilities; the preservation solution can meet the requirements of cell morphology examination and molecular detection at the same time, double-detection compatibility is achieved, the preparation technology is simple and convenient, and large-scale production is easy to achieve.

Methods for obtaining enucleated cells from nucleated cells are described herein. Methods for cell processing, comprising providing a composition containing nucleated cells and enucleating at least a portion of nucleated cells to produce an enucleated cell fraction, are also described herein. Methods for cell processing, comprising expressing heterologous gene products, are also described herein. Pharmaceutical compositions containing enucleated cells are also provided.

Methods and compositions useful for delivering a protein to a plurality of nuclei in a cell. Fusion proteins comprising one or more signals useful for promoting delivery of the proteins to a plurality of nuclei in a multinucleate cell. Methods comprising administering isolated nucleic acids to a multinucleate cell, wherein the isolated nucleic acid comprises a sequence encoding a fusion protein, the fusion protein comprising, a protein of interest fused to at least the following migration signals: (a) at least one nuclear export signal (NFS); and (b) at least one nuclear localization signal (NLS) and / or at least one nucleolar localization signal (NoLS). Fusion proteins comprising: (a) a protein of interest; and (b) at least the following migration signals: (i) a nuclear export signal (NFS); (ii) a nuclear localization signal (NLS); and (iii) a nucleolar localization signal (NoLS). Methods of making and using the same. Methods of delivering recombinant genes and / or fusion proteins to multinucleate cells.

According to the coelomic fluid evaluation and nucleated cell detection AI training and detection method and device, a coelomic fluid sample is preprocessed, and a microscopic examination sample is obtained; tiling a microscopic examination sample; shooting the tiled microscopic examination sample to obtain a microscopic examination sample image; identifying and labeling nucleated cells in the microscopic examination sample image to obtain a labeled picture; the annotation pictures comprise granulocyte annotation pictures, macrophage annotation pictures and lymphocyte annotation pictures; and carrying out AI training by using the labeled picture to obtain a coelomic fluid nucleated cell detection AI feature data set, wherein the AI recognition algorithm comprises coelomic fluid nucleated cell feature data. By means of microscopic image AI analysis, accurate identification and quantitative analysis are carried out, the content and the proportion of nucleated cells in the body cavity fluid are output, and new body cavity fluid parameters are provided clinically to serve as reference.

The invention discloses a serous cavity effusion sample property identification method and system based on data statistics, and the method comprises the following steps: S1, preparing a serous cavity effusion sample slide, the operation comprising manual slide pushing and dyeing; s2, checking the prepared slide, wherein the checking content comprises whether cell distribution is uniform or not and whether dyeing is clear or not, and re-flaking the sample of which the flaking quality does not meet the item checking requirement; s3, observing the image of the slide under the microscope, judging the type and number of cells according to the serous cavity effusion cell morphological map, and recording the result; s4, carrying out statistical analysis on a recording result obtained in the step S3, and calculating the proportion of the total number of cancer cells in all the visual fields to the total number of nucleated cells in all the visual fields; and S5, judging the sample property according to the ratio and the number of the total number of cancer cells in the visual field. The invention provides a clear judgment rule and an implementation method of the sample property, and provides a clear judgment rule for the result in the gray region, so that the accuracy of the final result is ensured.

The invention relates to the technical field of medical treatment, and particularly discloses a red breaking liquid for bloody body fluid cell wax blocks. The red breaking liquid is prepared from absolute ethyl alcohol and distilled water, and the volume concentration of the absolute ethyl alcohol is 20%-30%; according to the method, the ethanol aqueous solution in a specific concentration range is adopted as a splitting system, efficient and selective splitting of the red blood cells in the bloody body fluid is realized, and cell membranes of the seedless red blood cells can be quickly destroyed by utilizing the dissolution effect of ethanol on cell membrane lipid and the hypotonic characteristic of the solution, so that contents of the seedless red blood cells are released and are easily eluted. Meanwhile, the morphological integrity and cell membrane antigenicity of nucleated cells (including tumor cells) can be protected to the greatest extent, and the problem of cell damage or antigen shielding possibly caused by a conventional hemolytic agent is avoided.

The invention discloses a cerebrospinal fluid cell morphology auditing process method and device, computer equipment and a storage medium, and relates to the technical field of cerebrospinal fluid cell morphology auditing, and the method comprises the steps: responding to the selection operation of a distribution diagram function module, carrying out the full-piece display of a cerebrospinal fluid cell image, and obtaining a cerebrospinal fluid cell image; the cerebrospinal fluid cell image is an image obtained by performing full scanning on a cerebrospinal fluid cell smear; in response to a frame selection counting button for the cerebrospinal fluid cell image, a cell frame selection interface is provided for a user to frame and select areas needing to be counted, and the cerebrospinal fluid cell image is displayed on the cell frame selection interface; and in response to a frame selection operation of a user, extracting nucleated cells existing in the frame selection area. According to the method, the nucleated cells in the frame selection area on the cerebrospinal fluid cell image are automatically extracted, counted, marked and classified under the distribution diagram function module, so that the target number of cerebrospinal fluid nucleated cells can be quickly and accurately identified.

The invention discloses a preparation method of a CTC capture detection kit based on a prostatic cancer specific marker, and relates to the technical field of circulating tumor cell detection. Screening to obtain a membrane protein marker combination which is stably co-expressed in circulating tumor cells and is not expressed in normal peripheral blood nucleated cells, wherein the membrane protein marker combination comprises at least two of PSMA, STEAP1 and NKX3-1; 2, aiming at each member in the membrane protein marker combination obtained in the step 1, screening through an SELEX (systematic evolution of ligands by exponential enrichment) technology to obtain a corresponding DNA (deoxyribonucleic acid) aptamer, and carrying out 5 '-terminal amino modification on the obtained DNA aptamer; 3, providing a solid-phase chip with a micropore array structure, and carrying out polyethylene glycol surface passivation treatment on the inner walls of micropores; and 4, mixing the amino-modified DNA aptamers obtained in the step 2, and fixing the amino-modified DNA aptamers on the inner walls of the passivated micropores obtained in the step 3 in a covalent coupling manner.

The invention discloses a method for separating mononuclear cells from alpaca peripheral blood, a preparation and a preparation method, and relates to the technical field of biology, the preparation comprises a reagent A and a reagent B, the reagent A comprises 1.0 mg / mL to 4.0 mg / mL of hydroxypropyl methylcellulose, 2.0 mg / mL to 6.0 mg / mL of chitosan quaternary ammonium salt, 48.0 mg / mL to 54.0 mg / mL of polysucrose 400 and 80.0 mg / mL to 10.0 mg / mL of meglumine diatrizoate; the reagent B comprises a Percoll separating medium, a diluent of the Percoll separating medium is normal saline or PBS, and the density of the Percoll separating medium is 1.079 g / mL-1. 081 g / mL. The problems that in the prior art, the density of neutrophils and the density of mononuclear cells in alpaca peripheral blood are close, the mononuclear cells are separated and extracted by using a single or discontinuous gradient separating medium, and the separation efficiency of the neutrophils and the mononuclear cells is low can be solved. And the effect is poor.

The invention relates to the technical field of biology, in particular to a composition for treating platelet-related diseases and a preparation method thereof. The preparation method comprises the following steps: culturing initial cells by using a culture medium added with a small-molecule inhibitor, and collecting the obtained cells to obtain the composition, wherein the small-molecule inhibitor comprises a histone methyltransferase inhibitor, or comprises a combination of the histone methyltransferase inhibitor and a JAK kinase inhibitor; the starting cells are selected from one or more of total nucleated cells and mononuclear cells. The composition prepared by the preparation method can simultaneously realize in-vivo effects of timely supplement and relatively long-term continuous supply of platelets.

Embodiments of this disclosure relate to methods and compositions relating to the optimization and selection of umbilical cord blood units for the production of immune cells, such as natural killer (NK) cells, for use in adoptive cell therapy. In certain embodiments, specific properties of an umbilical cord blood unit and / or the properties of cells obtained therefrom are analyzed. If a threshold measurement is met for one or more properties of the umbilical cord blood unit and / or the properties of cells obtained therefrom, the umbilical cord blood unit is used as a source for the production of immune cells. Specific properties to be measured include, for example, the viability of umbilical cord blood cells, the time from birth to cryopreservation of the umbilical cord blood unit, the total nucleated cell recovery rate, infant weight, infant sex, maternal age, gestational age, CD34-positive cell percentage, and / or nucleated red blood cell content. These properties may be determined before and / or after cryopreservation.

This invention discloses the application of hemp seed oil in the preparation of drugs for the prevention or mitigation of acute ionizing radiation-induced diseases, relating to the field of biomedical technology. This invention discovers that hemp seed oil can protect the intestinal epithelium and crypt structures under acute high-dose irradiation, maintaining the intestinal barrier and digestive function. Simultaneously, it can protect splenic immune function by slowing the reduction in the white pulp region of the spleen and increasing the number of nucleated cells and lymphoid immune cells in the spleen, thereby significantly improving the individual survival rate after lethal doses of radiation, providing a new approach for treating acute ionizing radiation-induced diseases.

The application relates to a DLBCL tumor cell intelligent detection method and system in bone marrow smears, which comprises the following steps: acquiring a full-field digital pathology image of a bone marrow smear; performing overlapping block processing to obtain a plurality of sub-image blocks; performing region of interest screening on the sub-image blocks to retain ROI image blocks containing effective cells; performing standardization processing on the ROI image blocks to obtain a target detection input image; detecting the target detection input image by using a deep learning target detection model to output a bounding box target containing DLBCL tumor cells and other cells; performing edge filtering processing on the bounding box target to eliminate the bounding box corresponding to the edge incomplete cells; counting the number of DLBCL tumor cells and other cells; calculating the proportion of DLBCL tumor cells in all nucleated cells to output a detection result. The application realizes automatic identification and quantitative analysis of DLBCL cells, has small calculation overhead, and has a high recognition rate for cross-block cells.

The present invention relates to a nucleated cell death induction method in which a low temperature treatment is performed on incubating blood cells such that only nucleated cells are damaged and enucleated blood cells are not damaged. According to the present invention, nucleated cell death can be induced by means of a simple, cost-effective and safe method.

The invention relates to a bone marrow cell image sample generation method and device, computer equipment and a computer readable storage medium, and the method comprises the steps: obtaining a simulated background / red blood cell segmentation binary image randomly generated by a generative adversarial network, and inputting the simulated background / red blood cell segmentation binary image as a constraint condition into a conditional generative adversarial network; acquiring a simulated background / red blood cell RGB image generated by the conditional generative adversarial network; obtaining a simulated karyocyte probability distribution function generated by a variational auto-encoder; performing random sampling on an image corresponding to the simulated nucleated cell probability distribution function according to a preset number, and determining coordinate positions of nucleated cells in a bone marrow cell image sample to be generated; and pasting the nucleated cell cut image in the bone marrow cell smear image to the coordinate position corresponding to the simulated background / red blood cell RGB image to generate a bone marrow cell image sample, thereby effectively improving the bone marrow cell image sample generation efficiency.