18 results about "Nucleated cell" patented technology

The application provides a method for detecting HES1 markers of melanoma CTCs by using multiplex immunofluorescence technology, and the method comprises the following steps: S1, collecting peripheral blood of a melanoma patient, and performing sorting and enrichment of CTCs through a microfluidic chip; S2, fixing and permeating after making all nucleated cells in the blood flatly and enriched on an adherent slide through a centrifuge; and sequentially performing co-incubation of HES1, HMB45 and CD45 antibodies on the screened melanoma CTCs through the multiplex immunofluorescence technology, and further analyzing the expression of the HES1 antibody and performing image analysis and quantification. The method is simple in operation, high in detection accuracy, can avoid false negatives and the like, and improves the screening accuracy of melanoma CTCs.

The utility model discloses a rapid mononuclear cell separation tube capable of being easily filled with a partition plate, and belongs to the technical field of cell separation, the rapid mononuclear cell separation tube comprises a centrifugal tube and the partition plate provided with three supporting legs, the partition plate is provided with a peripheral through hole, and the side wall of the bottom in the centrifugal tube is provided with a flange surrounding the axis of the centrifugal tube; a supporting cover is arranged at the open end of the centrifugal tube, a supporting rod moving in the axis direction of the centrifugal tube is arranged on the supporting cover, and a tray used for supporting the partition plate and driving the partition plate to move relative to the centrifugal tube in parallel is arranged at the end, located in the centrifugal tube, of the supporting rod. The tray is provided with a protrusion matched with the peripheral through hole, the protrusion is provided with an air hole which is the same as the peripheral through hole and penetrates through the tray, and the device has the advantages that the partition plate can be rapidly and accurately filled under the condition that it is guaranteed that the partition plate is relatively parallel to the inner diameter of the centrifugal tube, the filling efficiency is greatly improved, and the partition plate and the centrifugal tube are firmly fixed.

The utility model relates to a cord blood hematopoietic stem cell collection, preparation and cryopreservation integrated quadruple bag which comprises a blood collection bag, a preparation bag, a plasma bag and a cryopreservation bag, and the blood collection bag is connected with the preparation bag through a first catheter and a first three-way pipe; a first flow stopping clip is arranged on the second catheter connected between the preparation bag and the first three-way pipe; the first three-way pipe is respectively connected with the plasma bag and the cryopreservation bag through a second conduit and a second three-way pipe; a second flow stopping clip is arranged on the second catheter; a third flow stopping clip is arranged on the fourth catheter connected between the plasma bag and the second three-way pipe; and a fourth flow stopping clip is arranged on the fifth catheter connected between the second three-way pipe and the cryopreservation bag. The quadruple bag is more convenient to extract cells, the cost is reduced, the consumed time is less, the recovery rate of nucleated cells is high, and the separation effect is better.

This invention provides a means to obtain more clinically useful information when analyzing particles contained in blood samples using metachromatic positive dyes. In a particle analysis method for analyzing particles contained in blood samples, particles are stained with a metachromatic positive dye; the stained particles are irradiated with light; the intensity of a first fluorescence from the stacking component of the metachromatic positive dye and the intensity of a second fluorescence from the embedded component of the metachromatic positive dye are measured, the first and second fluorescence being emitted by each particle contained in the blood sample; the intensities of the first and second fluorescence emitted by each particle are normalized according to the size of each particle to obtain the fluorescence concentration of each of the first and second fluorescence in each particle; in a two-dimensional plot of the normalized fluorescence concentrations, each particle is clustered into multiple particle clusters, including at least two of erythrocyte clusters, platelet clusters, and nucleated cell clusters; and a histogram is created where the intensity of the first fluorescence is a histogram of the RNA amount of the class and the intensity of the second fluorescence is a histogram of the DNA amount of the class included in at least one of the multiple particle clusters.

A method for obtaining enucleated cells from nucleated cells is described herein. A cell processing method is also described herein, which includes providing a composition containing nucleated cells and enucleating at least a portion of the nucleated cells to produce an enucleated cell fraction.

Described herein are methods for obtaining enucleated cells from nucleated cells. Also described herein are methods for cell processing, including providing a composition containing nucleated cells and enucleating at least a portion of the nucleated cells to produce an enucleated cell fraction. Also described herein are methods for cell processing, including expressing the heterologous gene product. Also provided are pharmaceuticals compositions comprising an enucleated cell.

The utility model discloses a mononuclear cell separation bag, the open end of a whole blood sample bag is provided with a liquid one-way flow conduit and is connected with a blood sample transfer conduit, a plasma transfer conduit and a blood cell transfer conduit, the whole blood sample bag is connected with a plasma bag through the plasma transfer conduit, the open end of the plasma bag is provided with the liquid one-way flow conduit, and the blood cell transfer conduit is connected with the blood cell transfer conduit. The blood sample transfer catheter is connected with the plasma transfer catheter and the blood cell transfer catheter, the other end of the blood cell transfer catheter is connected with the blood cell transfer catheter, the other end of the blood cell transfer catheter is connected with the blood cell transfer bag, the open end of the blood cell transfer bag is provided with a sample extraction port, and the blood sample transfer catheter, the plasma transfer catheter and the blood cell transfer catheter are provided with disposable pipeline plugging devices and pipeline temporary sealing devices. The disposable closed container is used, so that the centrifugal tube is prevented from being frequently replaced, and meanwhile, the experiment failure caused by sample pollution due to the fact that hands of operators or articles pass through the upper part of the open centrifugal tube is also avoided.

The utility model discloses a kind of nucleated cell kit canning device, it is related to reagent kit packaging technical field.The utility model includes support frame, further include: support part, the support part is set on support part;Filling part, the filling part is set on the top of support part;And blanking part, the blanking part is set on support part;Wherein, the bottom of support frame is equipped with motor slot, and the structure on the filling part is compatible with this motor slot.The utility model sets up filling part, when using, operating personnel first places the reagent kit to be filled in on feed conveyor belt, feed conveyor belt is sequentially conveyed to equidistant turntable according to pre-set program, at this time, motor one starts, and equidistant turntable is rotated by rotating shaft one drive, to avoid interference, feed conveyor belt pauses conveying during equidistant turntable operation, ensure that equidistant turntable can move reagent kit to the just below of adjusting plate on feed hopper.

The invention relates to the technical field of sample preservation. The invention provides an exfoliated cell preserving fluid and a preparation method and application thereof, the preserving fluid comprises a cracking agent, a buffering agent, a chelating agent, a mucus dissolving agent and a preservative, the cracking agent and the buffering agent comprise ammonium salt, and the ammonium salt comprises at least one selected from the group consisting of ammonium chloride, ammonium acetate and ammonium bicarbonate. The preserving fluid can selectively lyse red blood cells to reduce blood background interference, and strongly disperse and dissolve mucus to effectively release wrapped target cells; the morphological integrity and nucleic acid integrity of nucleated cells are protected, and morphological abnormalities such as solid shrinkage, dissolution or expansion of the cells are avoided. The biological stability of the sample in the long-term storage and transportation process is ensured due to strong anti-corrosion and antibacterial capabilities; the preservation solution can meet the requirements of cell morphology examination and molecular detection at the same time, double-detection compatibility is achieved, the preparation technology is simple and convenient, and large-scale production is easy to achieve.

Methods for obtaining enucleated cells from nucleated cells are described herein. Methods for cell processing, comprising providing a composition containing nucleated cells and enucleating at least a portion of nucleated cells to produce an enucleated cell fraction, are also described herein. Methods for cell processing, comprising expressing heterologous gene products, are also described herein. Pharmaceutical compositions containing enucleated cells are also provided.

Methods and compositions useful for delivering a protein to a plurality of nuclei in a cell. Fusion proteins comprising one or more signals useful for promoting delivery of the proteins to a plurality of nuclei in a multinucleate cell. Methods comprising administering isolated nucleic acids to a multinucleate cell, wherein the isolated nucleic acid comprises a sequence encoding a fusion protein, the fusion protein comprising, a protein of interest fused to at least the following migration signals: (a) at least one nuclear export signal (NFS); and (b) at least one nuclear localization signal (NLS) and / or at least one nucleolar localization signal (NoLS). Fusion proteins comprising: (a) a protein of interest; and (b) at least the following migration signals: (i) a nuclear export signal (NFS); (ii) a nuclear localization signal (NLS); and (iii) a nucleolar localization signal (NoLS). Methods of making and using the same. Methods of delivering recombinant genes and / or fusion proteins to multinucleate cells.

The invention relates to the technical field of medical treatment, and particularly discloses a red breaking liquid for bloody body fluid cell wax blocks. The red breaking liquid is prepared from absolute ethyl alcohol and distilled water, and the volume concentration of the absolute ethyl alcohol is 20%-30%; according to the method, the ethanol aqueous solution in a specific concentration range is adopted as a splitting system, efficient and selective splitting of the red blood cells in the bloody body fluid is realized, and cell membranes of the seedless red blood cells can be quickly destroyed by utilizing the dissolution effect of ethanol on cell membrane lipid and the hypotonic characteristic of the solution, so that contents of the seedless red blood cells are released and are easily eluted. Meanwhile, the morphological integrity and cell membrane antigenicity of nucleated cells (including tumor cells) can be protected to the greatest extent, and the problem of cell damage or antigen shielding possibly caused by a conventional hemolytic agent is avoided.

The invention discloses a preparation method of a CTC capture detection kit based on a prostatic cancer specific marker, and relates to the technical field of circulating tumor cell detection. Screening to obtain a membrane protein marker combination which is stably co-expressed in circulating tumor cells and is not expressed in normal peripheral blood nucleated cells, wherein the membrane protein marker combination comprises at least two of PSMA, STEAP1 and NKX3-1; 2, aiming at each member in the membrane protein marker combination obtained in the step 1, screening through an SELEX (systematic evolution of ligands by exponential enrichment) technology to obtain a corresponding DNA (deoxyribonucleic acid) aptamer, and carrying out 5 '-terminal amino modification on the obtained DNA aptamer; 3, providing a solid-phase chip with a micropore array structure, and carrying out polyethylene glycol surface passivation treatment on the inner walls of micropores; and 4, mixing the amino-modified DNA aptamers obtained in the step 2, and fixing the amino-modified DNA aptamers on the inner walls of the passivated micropores obtained in the step 3 in a covalent coupling manner.

The invention discloses a method for separating mononuclear cells from alpaca peripheral blood, a preparation and a preparation method, and relates to the technical field of biology, the preparation comprises a reagent A and a reagent B, the reagent A comprises 1.0 mg / mL to 4.0 mg / mL of hydroxypropyl methylcellulose, 2.0 mg / mL to 6.0 mg / mL of chitosan quaternary ammonium salt, 48.0 mg / mL to 54.0 mg / mL of polysucrose 400 and 80.0 mg / mL to 10.0 mg / mL of meglumine diatrizoate; the reagent B comprises a Percoll separating medium, a diluent of the Percoll separating medium is normal saline or PBS, and the density of the Percoll separating medium is 1.079 g / mL-1. 081 g / mL. The problems that in the prior art, the density of neutrophils and the density of mononuclear cells in alpaca peripheral blood are close, the mononuclear cells are separated and extracted by using a single or discontinuous gradient separating medium, and the separation efficiency of the neutrophils and the mononuclear cells is low can be solved. And the effect is poor.

Embodiments of this disclosure relate to methods and compositions relating to the optimization and selection of umbilical cord blood units for the production of immune cells, such as natural killer (NK) cells, for use in adoptive cell therapy. In certain embodiments, specific properties of an umbilical cord blood unit and / or the properties of cells obtained therefrom are analyzed. If a threshold measurement is met for one or more properties of the umbilical cord blood unit and / or the properties of cells obtained therefrom, the umbilical cord blood unit is used as a source for the production of immune cells. Specific properties to be measured include, for example, the viability of umbilical cord blood cells, the time from birth to cryopreservation of the umbilical cord blood unit, the total nucleated cell recovery rate, infant weight, infant sex, maternal age, gestational age, CD34-positive cell percentage, and / or nucleated red blood cell content. These properties may be determined before and / or after cryopreservation.

This invention discloses the application of hemp seed oil in the preparation of drugs for the prevention or mitigation of acute ionizing radiation-induced diseases, relating to the field of biomedical technology. This invention discovers that hemp seed oil can protect the intestinal epithelium and crypt structures under acute high-dose irradiation, maintaining the intestinal barrier and digestive function. Simultaneously, it can protect splenic immune function by slowing the reduction in the white pulp region of the spleen and increasing the number of nucleated cells and lymphoid immune cells in the spleen, thereby significantly improving the individual survival rate after lethal doses of radiation, providing a new approach for treating acute ionizing radiation-induced diseases.

The present invention relates to a nucleated cell death induction method in which a low temperature treatment is performed on incubating blood cells such that only nucleated cells are damaged and enucleated blood cells are not damaged. According to the present invention, nucleated cell death can be induced by means of a simple, cost-effective and safe method.

The invention relates to a bone marrow cell image sample generation method and device, computer equipment and a computer readable storage medium, and the method comprises the steps: obtaining a simulated background / red blood cell segmentation binary image randomly generated by a generative adversarial network, and inputting the simulated background / red blood cell segmentation binary image as a constraint condition into a conditional generative adversarial network; acquiring a simulated background / red blood cell RGB image generated by the conditional generative adversarial network; obtaining a simulated karyocyte probability distribution function generated by a variational auto-encoder; performing random sampling on an image corresponding to the simulated nucleated cell probability distribution function according to a preset number, and determining coordinate positions of nucleated cells in a bone marrow cell image sample to be generated; and pasting the nucleated cell cut image in the bone marrow cell smear image to the coordinate position corresponding to the simulated background / red blood cell RGB image to generate a bone marrow cell image sample, thereby effectively improving the bone marrow cell image sample generation efficiency.