The utility model discloses a positioning device for
tail puncture of laboratory mice, which comprises a needle cylinder, and a push rod is arranged in the needle cylinder. The bottom side of the needle cylinder is fixedly connected to the connector; a needle head is detachably connected in the connector; the outer side of the connector is sleeved with a positioning device, and the positioning device comprises a positioning cylinder, an adjusting cylinder and a spreader; the positioning cylinder is fixedly sleeved on the outer side of the connector, and scales are arranged on the outer wall of the positioning cylinder; a gap is reserved between the inner wall of the adjusting cylinder and the outer wall of the needle head, and the bottom end face of the adjusting cylinder is flush with the bottom end face of the needle point. The bottom side of the adjusting cylinder is sleeved with the spreader. Through the cooperation of the positioning cylinder and the adjusting cylinder, the exposed length of the needle head each time can be adjusted, so that when experimenters respectively puncture the tails of a plurality of white rats, the inserted lengths of the needle head are kept consistent; in combination with the use of the
dilator, the epidermal tissue wound punctured by the
tail of the
white mouse can be slightly expanded outwards, so that the
irradiation of more light rays is facilitated, and the solidification of a
tissue engineering scaffold material is accelerated.