49 results about "White mouse" patented technology

The invention provides a method for producing pure amino acid chelate and use thereof. The method is as followings: the first step is to reacting oxide or metal hydroxide and the amino acid to generate hydroxyl amino acetate; the second step is to conduct a combination reaction of the hydroxyl amino acetate which is generated in the first step and at least an amino-containing compound, and the compound with abundant amino is reacted with a metal ion, thus, the generated amino acid chelate structure is formed by combining one metal ion and a plurality of ligands of the amino acid, but the number of the ligands of the amino acid is equal to the number of the ligands of the metal ion, and the pure amino acid chelate is separated from reaction generation liquid. The second step also comprises several continuous sub-steps for reaction with a plurality of amino-containing compounds. The generated pure amino acid chelate can be applied to beverage, food, health products and medicine, in particular a zinc amino acid chelate that can obviously and effectively increase a white mouse immune function with lower immunity.

The invention belongs to the technical field of veterinary medicaments and new biological medicaments and relates to a porcine circovirus-II vaccine potency test method. The method comprises: immunizing a white mouse with a porcine circovirus-II inactivated vaccine; and determining the PCV2 specific antibody content of the serum of the white mouse to evaluate the potency of the vaccine. When the vaccine potency test method is used, and the problems of time-consuming and labor-consuming test pig screening, large inter-batch test result difference and the like of the conventional porcine circovirus-II inactivated vaccine potency test are solved. The test method of the invention has the advantages of short test period, simple operation, low cost, accurate result and high repeatability. The method has a high application value and a promising market prospect.

The invention provides a formula of an anti-fatigue Chinese medicinal composition and anti-fatigue lagehead atractylodes paste prepared by the formula. The anti-fatigue Chinese medicinal composition comprises the following components in part by mass: 400 to 600 parts of lagehead atractylodes, 100 to 300 parts of fragrant solomonseal rhizome and 100 to 150 parts of tangerine peel. Compared with other lagehead atractylodes paste formulas, except for keeping the effects of treating incoordination between the spleen and the stomach, poor appetite, diarrhea and the like of the pure lagehead atractylodes paste, the anti-fatigue lagehead atractylodes paste formula mainly adopts the fragrant solomonseal rhizome having the effects of invigorating yin, invigorating 5 kinds of strains and 7 kinds of impairments, regulating blood and qui, making people stronger and the like, so that the anti-fatigue effect of the formula is highlighted. An lagehead atractylodes paste physical fatigue alleviating function test conducted by the Jinhua Food and Drug Test Institute shows that the lagehead atractylodes paste formula has an extremely obvious anti-fatigue effect, the paste prolongs the longest swimming time of a white mouse test animal by 451.3 percent compared with negative control(with no medicament), and prolongs the longest swimming time by 91.8 percent compared with the lagehead atractylodes paste without a fragrant solomonseal rhizome component.

The invention provides a Chinese medicinal preparation for preventing and treating radiation and a preparation method thereof and relates to the Chinese medicinal preparation for treating and preventing radiation injury and the preparation method thereof. The Chinese medicinal preparation is specifically prepared from heterophylly falsestarwort root, indian buead, bighead atractylodes rhizome, liquorice, Szechuan lovage rhizome, Chinese angelica, red paeony root, rehmannia root, barbed skullcap herb, honeysuckle, mulberry leaf, hairyvein agrimonia herb, medlar and green tea in a certain proportion. Pharmacodynamic experiments carried out by adopting an animal model with reduced tangible hemocytes and nucleated cells in bone marrow of a white mouse due to 60Co-gamma rays show that the preparation has a good effect on hemocytes and hemopoietic tissues injured by nuclear radiation.

The inventin is named a method for extraction of N6-(2-hydroxyethyl) adenosine from paecilomyces cicadae culture. The invention consists of seperating and breeding new bacterial strain APC-20 of paecilomyces cicadae which is of strong livabitity, high yield of metabolically active compound and good quality from the nature; presenting a method for industrialized solid culture, fluid culture or fermentation production; then eatracting analgesic compounds contained in the said fermented product with 0.36 % of extraction rate and every significant part and providing a method for preparing polytypic species of analgesic drug; the compound has 99 % of analgesic rate examined by little white mouse-twist method. The analgesic mechanism of this compound is different from the presently used opium analgesic agent, and is of no addiction and safe, so it has a broad development and application propect.

The invention discloses a tree polymer target contrast agent and preparation method thereof, and the contrast agent is nanoparticle, which has the structure as follows: a target radical T is located on the outerest end; a hydrophilic polyethylene glycol chain is used as a connecting arm; a tree polymer PAMAM is located in the central nucleus; paramagnetic metal X is imaged in vivo; the general formula thereof is PAMAM-PEG[(n)]-T / X. The preparation method has the steps of: PEG with different molecular masses is reacted with the target radical for preparing a product with a target radical on one end and a amino on the other end: NH[2]-PEG[(n)]-T; NH[2]-PEG[(N)]-T is reacted with PAMAM-COOH with molar ratio 32:1 for preparing a macromolecular ligand; T-PEG[(N)]-PAMAM; The ligand is chelated with paramagnetic metallic ion solution for preparing PAMAM- PEG[(N)]-T / X. The preparation method provided by the invention has the advantages of simple operation, mild and safe condition and wide application. And the prepared contrast agent has small cell toxicity, high relaxivity, and very good imaging effect for white mouse in vivo; obvious target property for imaging of tumor - bearing nude mouse in vivo.

The invention discloses an eprinomectin transdermal agent, a preparation method and application. The eprinomectin transdermal agent is composed of azone, oleic acid, propylene glycol, dimethyl sulfoxide and alcohole with a certain weight amount. The preparation method comprises: A, performing sample preparation, namely, setting five levels for each transdermal promoting agent, and adding alcohol for preparing samples; B, performing in-vitro transdermal experiments, fixedly disposing skin of a white mouse between a dispersion pool and a receiving pool of a YB-P6 intelligent transdermal tester, enabling the inner layer surface of skin to face to the receiving pool, enabling the whole dispersion pool to be full with the eprinomectin transdermal-agent formula sample, and adding acetonitrile to fill the receiving pool as a receiving solution; C, detecting the receiving solution obtained in the step B by using high performance liquid chromatographic instrument, and selecting a formula through visual analysis and variance analysis on an orthogonal experiment result; D, preparing the formula and verifying according to the front steps; and E, preparing a final product, and preparing a transdermal agent containing 0.025-0.1 g of eprinomectin. The formula is reasonable, convenient to use and capable of continuously expelling parasites without needing withdrawal time, and has the expelling rate of 90% or more on nematode and ectoparasites.

The invention provides a ractopamine and cimaterolm combined colloidal gold test strip, and a preparation method and an application thereof. The test strip comprises a base plate with a first end and a second end. A filter paper, a sample pad, a gold label pad, a nitrocellulose membrane, and a water absorption pad are sequentially formed on the base plate from the first end to the second end. The gold label pad comprises colloidal-gold-labeled anti-ractopamine monoclonal antibody and colloidal-gold-labeled anti-cimaterolm monoclonal antibody. Two test lines and a control line are further formed on the nitrocellulose membrane. The test lines are sequentially composed of a ractopamine detection antigen linear point sample which can bind with the anti-ractopamine monoclonal antibody, and a cimaterolm detection antigen linear point sample which can bind with the anti-cimaterolm monoclonal antibody. The control line is composed of a donkey-anti-white-mouse antibody linear point sample which can bind with the anti-ractopamine monoclonal antibody and the anti-cimaterolm monoclonal antibody. With the cooperation of the test strip and a colloidal gold reading instrument, ractopamine and cimaterolm can be effectively and quantitatively detected.

The invention provides an adjustable white mouse dissecting tale. The table comprises a top plate, a bottom plate, an adjusting bolt, a first locking bolt, a four-leg press plate, a head press plate and second locking bolts. A connecting groove for installing the bottom plate is arranged at the other end of the top plate. The bottom plate can move along the connecting groove. The adjusting bolt passes through a thread hole to be connected with the bottom plate. The bottom of the top plate and the bottom of the bottom plate are both provided with magnets. The top of the top plate and the top of the bottom plate are both provided with positioning holes for installing the second locking bolts. Positioning openings are symmetrically arranged at two ends of a head press plate. The head press plate is installed at the top of the top plate through the first locking bolt. The adjustable white mouse dissecting table has the advantages of being simple in structure, convenient to use, capable of adjusting the dissecting table in a fast mode based on the specification of the white mouse, and fixing the head and four legs of the white mouse fast. The single component is simple in shape, and therefore the table is convenient to be assembled and dissembled for cleaning and disinfection.

The invention discloses a fixator for a white mouse. The fixator comprises a cylinder, a circular truncated cone, a tightening device, a clamping device and a support. The cylinder and the circular truncated cone are hollow. The interior of the cylinder is provided with bosses. The cylinder penetrates through the tightening device provided with a fixing platform and bolts. The fixing platform is in threaded connection with bolts. The bolts penetrate through the cylinder and are provided with clamping arms, on which silicon rubber cases are arranged. The clamping device is arranged in the cylinder and provided with a clamping wing and torsion springs. The torsion springs are mounted to the clamping wing and the cylinder. The support is installed below the tightening device and detachably arranged. The fixator for the white mouse has following advantages: vital signs of the white mouse can be well observed; and injection or the experiment is not affected when the white mouse escapes or struggles during a biological experiment for injection.

The invention discloses a method for constructing an animal model of skin ulcer with diabetes. A Kunming white mouse is chosen and the abdominal cavity is injected with alloxan reagent, the mid lower portion of the back is unhaired, sterilized and cut deep to be under the skin, then the white mouse is fed in a single case and fixed amount of water and food is supplied. After fast for 12 hours, blood is taken from the tail of the white mouse and the blood sugar value is tested by a blood sugar tester to be properly more than or equal to 10.0mmol / L. The statistical analysis of body surface wound observation and the histological wound observation of the experimental animal model proves that the white mouse model of skin ulcer with diabetes that is constructed by alloxan is feasible and can be taken as the study model of pathogenesis and drug effect mechanism of skin ulcer with diabetes. Compared with the prior art, the white mouse model of skin ulcer with diabetes constructed by the invention has the advantages of simple model constructing method, high model forming ratio, low cost, short cycle and good reproducibility, etc.

The invention discloses a method for constructing a diabetes mellitus animal model, which comprises the following steps: an alloxan reagent is injected in the abdominal cavity of a selected Kunming white mouse; then the white mouse is fed in a cage independently; water and food are fed to the white mouse quantitatively; after the white mouse is forbidden to eat food for 12 hours, blood is taken from the tail of the white mouse; a blood sugar meter is used for detecting a blood sugar value, and when the blood sugar value is larger than or equal to 10.0 mmol / L, the steps are ended. Proved by a statistical analysis of a blood sugar measuring result of the diabetes mellitus animal model, the method for constructing a white mouse diabetes mellitus model by utilizing the alloxan is feasible, and the model can be used as a research model of the pathogenesis and the drug mechanism of diabetes mellitus. Moreover, compared with the prior art, the constructed while mouse diabetes mellitus model has the advantages of simple model constructing method, high model forming rate, low cost, short period, good reproducibility, and the like.

The invention discloses an air quality measuring and controlling device for a leukemia model nursing ward. The air quality measuring and controlling device comprises a model ward, a front plate, a control panel and a first electric telescopic rod, wherein the left side of the front surface of the model ward is fixedly provided with an observation window in an inlaid manner, the front plate is fixedly mounted on the front surface of the model ward at the right side of the observation window, and the control panel is fixedly mounted on the front surface of the front plate. The air quality measuring and controlling device for the leukemia model nursing ward utilizes properties of a white mouse, drives the white mouse to move by means of a fluorescent lamp, and guides sterile and dust-free airinto the model ward, so that the model mouse and a leukemia patient are in a similar air environment, thereby improving the accuracy rate of sample performance features; and a number of bacteria falling on a culture medium can be used as a contrast of bacteria content inside the device, thereby being convenient for researchers to study the effect of the amount of bacteria in the air on the modelwhite mouse, and finally achieving the effect of improving the accuracy rate of research; and the air quality measuring and controlling device is simple in structure and reasonable in design, therebybeing easy to popularize.

The invention relates to the technical field of biotechnology, and provides a white mouse breeding bin for rescue and observation by judging biological activity through rotating speed. The white mouse breeding bin comprises a breeding bin, wherein the interior of the breeding bin is fixedly connected with a recognition mechanism, the bottom of the recognition mechanism is movably connected with an expansion mechanism, the left side and the right side of the expansion mechanism are movably connected with judgment mechanisms, and the top of the recognition mechanism is movably connected with a ventilation mechanism. According to the white mouse breeding bin for rescue and observation by judging biological activity through rotating speed, the magnetic force of an electromagnet changes continuously, a first iron block moves, the first iron block amplifies the distance of a rack plate through a lever, the rack plate drives a gear to rotate, the rotating speed of the gear is related to the carbon dioxide generation amount, the rotating speed of the gear is insufficient, centrifugal force borne by a sliding block is small, a contact switch is squeezed by the sliding block, the sliding block is squeezed to enable the alarm to give an alarm, and it is indicated that the carbon dioxide generation amount is too low, the white mouse life activity is low and observation and inspection are needed.

The present invention relates to the application of nonglandular purine in promoting wound healing, and nonglandular purine, such as guanosine, guanine and inosine, may be used in treating wound and promoting wound healing. The nonglandular purine can promote the healing of damaged skin of white mouse, health and suffering from congenital diabetes. During promoting wound healing, the nonglandular purine acts via activating the intracellular biological enzyme while the natto kinase inhibitor inhibits the biological effect of the compound.

A medicinal white mouse injection device comprises an upper half tube and a lower half tube, wherein two sides of the upper half tube are respectively connected with two sides of the lower half tube through two longitudinal elastic bands. The tube wall of the upper half tube and the tube wall of the lower half tube are respectively provided with a plurality of through holes. The medicinal white mouse injection device has advantages of convenient operation, simple structure and easy manufacture.

The invention relates to a white mouse tester, which consists of an uncovered square box, eight clapboards, a small square fence, a singlechip, an infrared correlation tube, a golden palm liquid crystal device, a power supply and a switch and is characterized in that: the uncovered square box is taken as a test place for a white mouse; the eight clapboards lean against one another pairwise to encircle four angles of the box into four small compartments; passages in which the white mouse can walk are formed on the middle parts of the four small compartments; a control display circuit which consists of the singlechip, the golden palm liquid crystal device, the power supply and the switch is arranged in the four small compartments respectively; and an emitting tube and a receiving tube are arranged on the clapboards on both sides of the passage so as to form the infrared correlation circuit. During testing, articles with different effects on the white mouse are arranged at the tail ends of the four passages, the white mouse which takes a medicament is put into the middle fence, then the fence is taken away, and the times when the white mouse walks to different ends during testing is recorded by the infrared correlation tube and is displayed by the liquid crystal device.

The invention discloses a feeding device for feeding a white mouse. The feeding device for feeding the white mouse comprises a U-shaped frame; a bottom plate is fixedly connected to the central position of the inner side of the U-shaped frame; the upper ends of the two sides of the inner walls of the U-shaped frame are fixedly connected with positioning strips; a placement plate is tightly bondedto the upper surface of the bottom plate and the lower surfaces of the positioning strips in a sliding manner; threaded holes are formed in the bottom plate; the inner sides of the threaded holes arein threaded connection with threaded knobs; threaded grooves are formed in the two sides in the lower end of the placement plate; the other end of each threaded knob is in threaded connection with theinner sides of the threaded grooves; a protective cover is tightly bonded to the upper end of the U-shaped frame; air changing holes are formed in the two sides of the protective cover; the inner sides of the air changing holes are fixedly connected with mesh plates. A feeding method comprises the following steps: cleaning and disinfecting; examining the appearance health of an animal; discriminating sex; preventing diseases; performing reproduction and population management. According to the device and the method disclosed by the invention, the assembling and the disassembling of the protective cover and a U-shaped seat are facilitated, so that internal space is cleaned more quickly; moreover, when a material is fed, the protective cover is not required to be opened, and the work of feeding the material can be completed by feeding through a conical hole directly.

The invention relates to a preparation method of a recombinant human albumin-uricase fusion protein drug. The recombinant human albumin-uricase fusion protein drug contains human albumin, pig uricase and a purification tag. The preparation method of the recombinant human albumin-uricase fusion protein drug has stable processes and is suitable for large-scale production. External enzyme activity detection and white mouse in-vivo preliminary pharmacodynamic experiments prove that the recombinant uricase can obviously reduce an internal uric acid concentration.

The invention relates to multi-functional smoke injury experiment equipment for a medical research. The multi-functional smoke injury experiment equipment comprises an injury chamber and a transmission device, wherein the injury chamber is a closed cavity; the transmission device is used for inputting or outputting white mice in an iron cage to the injury chamber; a smoke pot for generating white smoke and injuring the white mice in the iron cage in the injury chamber is arranged in the injury chamber; a monitoring device for monitoring whether fuel combustion of the smoke pot is complete or not, monitoring internal temperature and humidity of the injury chamber and the injury time of the white mice and detecting the ingredients of the white smoke is arranged in the injury chamber; and an adjusting device for adjusting the internal temperature and humidity of the injury chamber is arranged in the injury chamber. The multi-functional smoke injury experiment equipment has the beneficial effects that a military exercise site can be simulated through the equipment, the smoke pot combusts to generate the white smoke to cause inhalation injuries of the white mice, and the white mice reach the expected injuries and survival time, so that an economical, simple and stable small-animal model is provided for research on different degrees of inhalation injuries caused by the white smoke coming from the smoke pot.

The invention discloses a mouse pluripotent stem cell line for an erythroid specific high-expression GFP (Green Fluorescent Protein) and a construction method of the mouse pluripotent stem cell line.The mouse pluripotent stem cell line is prepared by the following steps: injecting an erythroid high-expression GFP vector into male pronucleus of Kunming white mouse fertilized eggs in a micro-injection manner, cultivating transgenic mice, verifying expressions of inserted genes at specific tissue cells, reproducing the transgenic mice, stabilizing the copy number of exogenous genes of generationmice of the transgenic mice, and preparing the mouse pluripotent stem cell line by blastaea. The mouse pluripotent stem cell line disclosed by the invention has important cytological value for researching in-vivo and in-vitro formation and evolution of blood cells, which contributes to uncovering the hematopoietic stem formation and complicated influence effect of the hematopoietic microenvironment and pushing the research process of differentiating pluripotent stem cells to transplantable hematopoietic stem cells in vitro.

The invention discloses a mouse cataract detection device, which includes a workbench and a mouse fixation device arranged on the workbench, and also includes: an electron microscope, which is installed on the workbench with an adjustable position, so as to obtain the mouse eye The magnified field of view; the display is electrically connected with the electron microscope to display the magnified image of the mouse eye; the eyelid support ring includes an elastic ring body, and two symmetrically arranged on the ring body and parallel to the The clamping body whose central axis of the ring body extends outwards, the natural relaxation state of the ring body under its own elastic force is a circle with a diameter of 3-7mm; An elbow clamp, and the end of the clamp head of the upper ring clamp is provided with a hole-shaped structure that is movably adapted to the clamping body of the eyelid support ring. By adopting the above mouse cataract detection device, it is possible to ensure easy, safe and accurate detection of mouse eye cataract.

The invention relates to a medicinal white mouse injecting fixator, and relates to the technical field of experimental animal injecting auxiliary equipment. The main purpose is to solve the technicalproblems that an existing sleeve with a pinhole cannot accurately align a to-be-injected part when injection is conducted on a medicinal white mouse and the injection efficiency is low. The fixator ischaracterized by comprising a base, a cover body which can be opened and closed relative to the base, a first fastener and a second fastener; the first fastener and the second fastener are both fastened on the base, and a first bayonet segment with adjustable aperture size and a second bayonet segment with adjustable aperture size are formed; in a first state, a first cover body, a second cover body and a third cover body are all closed relative to the base, an accommodating hole for accommodating the medicinal white mouse is formed in the fixator, and the accommodating hole comprises a firsthole segment formed between the first cover body and the base, a second hole segment formed between the second cover body and the base, a third hole segment formed between the third cover body and the base, the first bayonet segment and the second bayonet segment.

A medicinal white mouse injection device comprises an upper half tube and a lower half tube, wherein two sides of the upper half tube are respectively connected with two sides of the lower half tube through two longitudinal elastic bands. The tube wall of the upper half tube and the tube wall of the lower half tube are respectively provided with a plurality of through holes. The medicinal white mouse injection device has advantages of convenient operation, simple structure and easy manufacture.

An Internet of Things mouse behavior training device, including a cage body, which is equipped with an odor training device capable of training the mouse's sense of smell, a drinking water training device for training the mouse to drink water, an electric shock net for punishing the mouse, and a training net for training the mouse. A light generator for the light sensing ability of the white mouse, a sound generator and a control module for training the sound signal sensing ability of the white mouse. The invention can train the sensing ability of the mice to various smells, the drinking ability and habits of the mice, the sensitivity of the mice to light of different colors and intensities can be trained through the light generator, and the sounds of different frequencies and intensities can be emitted through the sound generator. Sound or music trains the auditory ability of mice. When the mice are not trained, the electric shock net is controlled by the control module, and the mice are given appropriate punishment. The trainer selects training items through the operation of the main control computer, and records the training data of the mice on the main control computer, and then compares and analyzes it with the data of the human brain.

The invention discloses an animal model feeding device, and relates to the field of animal model feeding. The animal model feeding device comprises a feeding box, wherein a sealing mechanism is arranged at the top end of the feeding box, a water supply mechanism is mounted at the top end of the sealing mechanism, a ventilation mechanism is mounted on the outer wall of the feeding box, a feeding bin is arranged in the feeding box, and a pulling box is mounted at the bottom end of the feeding bin and located on the inner wall of the feeding box. Through the pulling box, a transmission mechanism and a net lifting mechanism, when the feeding box needs to be cleaned, the pulling box is pulled firstly, the pulling box drives meshing teeth to rotate through multiple sets of pulling teeth arranged at the top end, the meshing teeth drive a winding wheel connected to one side to rotate, the winding wheel winds a pull rope, the pull rope changes the rotating direction along a fixed pulley and pulls up the net lifting mechanism, the net lifting mechanism lifts a white mouse out of the pulling box through multiple sets of silica gel pads arranged on the inner wall, and therefore, the problem that the white mouse needs to be moved to other positions in the cleaning process is solved.

The invention discloses an application of kainic acid in preparation of anti-cryptosporidium-baileyi medicines. An application method performed by the inventors comprises the following steps: preparing powder by using kainic acid and acceptable auxiliary materials, adding the powder into a feed, uniformly mixing, and feeding Lingnan yellow chicken according to a daily feed quantity, wherein the adding amount is that 4g of kainic acid needs to be added into per 100kg of the feed; or preparing kainic acid into water-soluble powder, dissolving the powder into water, and feeding a white mouse according to the daily water-drinking amount under the condition that the adding amount equals to dissolving 2g of kainic acid into per 100kg of water. Confirmed by animal infection experiments, the oocyst excreting quantity of cryptosporidium baileyi can be reduced by more than 78% by adding kainic acid; and kainic acid has relatively good anti-cryptosporidium effects.