123 results about "Animal testing" patented technology

The invention relates to a preparation method of an effective part extract of n-butyl alcohol of Eupatorium chinense and application of the effective part extract in treatment of diabetes complicated with depression. The technical scheme of the invention is to provide an extract of the n-butyl alcohol effective part of the eupatorium chinense, which can be used for treating diabetes complicated by depression diseases. In-vitro experiments show that the eupatorium chinense extract has an inhibition effect on acetylcholin esterase (AChE). In-vivo animal experiments find that classical anti-diabetes and depression treatment drugs, namely metformin hydrochloride and fluoxetine hydrochloride, are taken as positive controls, experimental research on the treatment effect of the effective part extract of the n-butyl alcohol of the eupatorium chinense on mice suffering from diabetes complicated with depression is developed, and research results show that the extract can regulate insulin resistance and can be used for treating diabetes complicated with depression. The glycometabolism of mice with diabetes mellitus complicated with depression is improved, and the depression symptom is improved. The extract has a remarkable effect of treating diabetes mellitus complicated with depression, has a good effect of treating diabetes mellitus complicated with depression, and is expected to be developed into a new natural medicine for treating diabetes mellitus complicated with depression.

The present invention provides a method and system for fabricating complex human tissues by integrating bio-electrospraying, cell electrospinning merged with 3D multi material microfluidic bioprinting, and future additions of magnetic and acoustic levitation technologies. The fabricated tissues serve as an alternative to animal testing in drug discovery processes, aiding in the prediction of human physiological responses to various drug compounds.

The invention discloses a liquid gate graphene transistor sensor for rapidly detecting a cell factor IFN-gamma, and a preparation method and application thereof. The sensor adopts an IFN-gamma aptamer to modify a gate on a liquid gate graphene field effect transistor (SSGT); during detection, after the aptamer on the grid captures an IFN-gamma target substance, the configuration is changed, the charge is changed, and then the Dirac point of a transfer curve is shifted, so that the target substance is detected respectively, and detection of IFN-gamma is finally realized by establishing a concentration model of a target system. According to the invention, the disadvantages of complex flow, insufficient adaptability in a multi-sample environment, lack of real-time monitoring capability and the like of the traditional detection means are solved, the rapid detection of one or more cytokines in blood can be realized through the combination and disassembly of the grids, the rapid detection method can be used for cell experiment detection and animal experiment detection, and the rapid detection method has a certain practical value.

The present invention relates to the technical field of the development and utilization of probiotics, specifically to a Weissella confusa DY12 with probiotic activity and its application. The Weissella confusa DY12 was isolated from sheep feces. The present invention identified the probiotic effect of Weissella confusa DY12 through animal experiments. The results showed that Weissella confusa DY12 could tolerate acid and bile salts and resist the gastrointestinal internal environment; its hydrophobicity was 39.47% and its auto-aggregation was 48.12%, demonstrating that the Weissella confusa DY12 of the present invention has good intestinal adhesion ability and has the potential of probiotics. The present invention verified that Weissella confusa DY12 can effectively regulate the immune function of the animal body, improve the antioxidant capacity of the body, as well as the content of lysozyme and the activity of alkaline phosphatase, and increase the daily weight gain, ultimately improving the growth performance of animals.

The invention provides an engineered bacterium outer vesicle drug delivery system capable of efficiently penetrating a blood brain barrier and targeting glioblastoma as well as a preparation method and application of the engineered bacterium outer vesicle drug delivery system, and belongs to the field of drug delivery. The engineering bacteria outer vesicle drug delivery system comprises double-layer phospholipid layer vesicles BEVs, a drug delivery system and a drug delivery system, wherein the particle size of the double-layer phospholipid layer vesicles BEVs is 20 The anti-tumor drug is wrapped in the BEVs, the surface of the BEVs is modified with functional polypeptide, and the functional polypeptide is at least one of Angiopep-2 and TAT cell penetrating peptide. Animal experiments verify that the synergistic effect of the Angiopep-2 and the TAT peptide can overcome the problem of modification saturation of the Angiopep-2 peptide, and significantly improve the ability of BEVs to penetrate through BBB and target tumor tissues. Therefore, the BEVs modified by the Angiopep-2 peptide and the TAT peptide have the advantages of multi-stage targeting, high efficiency and synergy, and can effectively deliver chemotherapeutic drugs to tumor tissues.

The invention discloses a laboratory animal behavior analysis method and system, and belongs to the technical field of animal behavior analys.The laboratory animal behavior analysis method includes the steps that A00, not less than three small animals are placed in a standard animal test box, and each small animal is dyed and distinguished; a01, the AI camera identifies and marks animal actions and postures and transmits marked video segments to the AI server; a02, the AI server carries out video reconstruction on the marked video segments to obtain 3D pictures, and repeated marks are removed; a03, the AI server counts the number of times of actions and postures of each animal. The AI camera carries out real-time edge processing on a shot video, animal postures and behaviors are recognized through an AI model library, and if it is judged that the postures and behaviors conform to actions and postures needing to be counted, relevant marks are synchronously marked in video stream signals; transmitting the marked video to an AI server; and the AI server performs screening statistics on the identification videos and gives a statistical result, so that the contrast experiment efficiency is improved.

The invention discloses a budeagfor inhalation dry powder inhalation which comprises active ingredients, a carrier and a dispersing aid, and the active ingredients are budesonide, glycopyrronium bromide and formoterol fumarate dihydrate which are subjected to particle size grading. According to the invention, different drugs can be delivered to respective target sites of the lung through a particle size grading process of micronized active ingredients. The budigegfu inhalation dry powder inhalation is good in delivery dose uniformity and stable in delivery efficiency, and animal test results show that the product prepared by adopting the active component size grading method has obvious advantages in the aspects of safety and effectiveness.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

The present disclosure relates to microbiome compositions for improving muscle strength using a heat-treated fermentative culture complex of KM2 Phytobacterium plantarum. The composition according to the present disclosure increases the synthesis of myoprotein and exhibits an effect of inhibiting the degradation of myoprotein. Animal tests show effects of improving muscle strength and increasing leg muscle mass by increasing grip strength, travel distance and speed, and results of improving popliteal muscle strength and increasing muscle mass even in clinical tests. Furthermore, it has been found that beneficial bacteria are increased by an increase in the diversity of the gut microbial flora, which varies as muscle decreases, and an improvement in the microbial flora composition, while harmful bacteria are reduced. Therefore, the composition can be used as a composition for preventing, alleviating or treating muscle diseases and a composition for improving muscle strength or increasing muscle mass.

The invention relates to an anti-VEGF antibody, a heavy chain variable region sequence is shown as SEQ ID NO: 1, and a light chain variable region sequence is shown as SEQ ID NO: 2. According to the present invention, the monoclonal antibody capable of specifically binding to VEGF is prepared, the binding of VEGF and its receptor can be inhibited after the monoclonal antibody is bound to VEGF, the downstream signal transduction pathway of VEGF is blocked, and the animal test results show that the antibody can inhibit the growth of glioblastoma.

The invention belongs to the field of medicine, and provides cream for treating pressure sores and diabetic feet and a preparation method thereof.The cream is prepared from a component A, a component B, a component C and a component D. The component A is prepared from, by mass, 10-40 parts of ligustrum sinense leaves, 10-40 parts of angelica sinensis, 10-40 parts of sanguisorba officinalis, 10-40 parts of golden cypress, 10-40 parts of liquorice, 10-40 parts of radix angelicae, 10-40 parts of polygonum cuspidatum and 10-40 parts of dried rehmannia glutinosa; the component B is prepared from the following components in parts by mass: 10 to 30 parts of rhizoma coptidis, 10 to 30 parts of radix et rhizoma rhei, 10 to 30 parts of radix paeoniae rubra, 10 to 30 parts of radix scrophulariae, 10 to 30 parts of cortex moutan, 10 to 30 parts of radix astragali seu hedysari and 10 to 30 parts of rhizoma chuanxiong; the component C is prepared from the following components in parts by weight: 10 to 15g of radix lithospermi, 3 to 10g of borneol and 5 to 10g of beewax; the component D is 500g of medical vaseline; through a specific traditional Chinese medicine composition, pressure sores and diabetic feet can be effectively treated, related symptoms can be remarkably relieved, and meanwhile animal experiments show that the traditional Chinese medicine cream is free of toxic and side effects.

The invention provides a method for evaluating eye irritation in vitro and application thereof, and belongs to the technical field of non-animal test methods. According to the method provided by the invention, a recombinant human corneal epithelium model is utilized, and relative tissue activity RTV and relative cell factor expression quantity RCL are used for comprehensively evaluating the eye irritation of a to-be-detected sample; the method specifically comprises the following steps: (1) pretreating a to-be-detected sample; (2) activity determination; (3) relative tissue activity judgment; (4) measuring the expression quantity of cell factors; (5) judging the expression quantity of relative cell factors; and (6) judging an eye irritation result. According to the method, the expression quantity of cell factors is increased to serve as the evaluation standard of the eye irritation, the evaluation accuracy of the eye irritation of the sample is remarkably improved, the samples which are judged to be free of eye irritation in relative tissue activity detection can be effectively recognized, then the attention of product developers and supervisors is aroused, and the health of a user is better protected.

The invention relates to a preparation method of a CpG ODN nano immune complex, which comprises the following steps: (1) uniformly mixing a protamine solution with a certain concentration and a CpG motif-containing oligodeoxynucleotide CpG ODN aqueous solution with a certain concentration according to a certain ratio, and carrying out electrostatic compounding assembly at room temperature to form a suspension; and (2) applying energy to the suspension to disperse / homogenize uniformly to form the suspension of the nano-composite. The nano-composite prepared by the method disclosed by the invention is controllable in particle size and controllable in surface charge. The nano particles formed under the preparation condition of an optimized proportion are uniformly dispersed, good in stability, good in resolubility after freezing, and good in long-term storage stability. The preparation method provided by the invention is simple, does not involve toxic and harmful chemical substances, and is suitable for amplification. Compared with the prior art, the preparation method provided by the invention can obviously improve the effective concentration of the effective active substance CpG ODN in the nano immune complex. The invention also relates to the nano immune complex prepared by the method and application thereof. Cell and animal experiments prove that the nano immune complex prepared by the method has good immunostimulatory activity, can stimulate the maturation of related immune cells and induce the secretion of related cytokines, can be independently used as an immunomodulator, and can also be used as an immunologic adjuvant to be combined with various antigens to be used as a vaccine preparation as a whole. The nano immune complex has good application prospects in the fields of inducing and activating immune cells to mature, releasing cytokines, resisting allergic diseases, preventing and treating microbial infectious diseases, vaccine adjuvants, resisting tumor immunotherapy and resisting Alzheimer's disease.

The invention discloses a traditional Chinese medicine composition for treating acute gout, an external gel and a preparation method thereof, and the traditional Chinese medicine composition comprises the following raw material components in parts by mass: 5-20 parts of radix clematidis, 5-20 parts of polygonum cuspidatum, 5-20 parts of honeysuckle stem, 5-15 parts of radix aconiti preparata, 5-15 parts of pine nodular branch, 1-5 parts of corydalis tuber and 1-3 parts of asarum. The gel disclosed by the invention is an external gel prepared by researching through a traditional Chinese medicine composition intermediate extraction process and a preparation forming process, has the effective effects of directly reaching an affected part, taking effect quickly, being good in patient compliance and the like, and can realize industrial production through pilot scale-up experiment verification. Animal experiment research results show that the radix clematidis pain-relieving gel has remarkable anti-inflammatory and pain-relieving effects.

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

This invention discloses a coat-enhancing composition for improving the quality of cat fur and its application, belonging to the field of pet food technology. The composition uses a high proportion of fresh chicken meat and fresh bone-in chicken thigh meat as the main protein source, combined with krill oil, fresh chicken liver, various natural plant powders, and compound nutrients, formulated in a scientific ratio. Animal experiments have verified that this invention can significantly increase the amino acid content of cat fur, improve fur luster and saturation, enhance tensile strength, and optimize hair cuticle structure, without adversely affecting the cat's weight, blood routine, blood biochemistry, or other health indicators, demonstrating safety and high efficacy. This invention can be widely applied to the preparation of functional fresh meat baked food for improving the quality of cat fur.

The invention provides an animal fixing device and animal test equipment. A first connecting assembly of the animal fixing device is connected to a supporting base; the second connecting assembly is connected to the supporting base. The running disc is arranged at the end, away from the supporting base, of the first connecting assembly and can rotate relative to the first connecting assembly. A groove part is formed in the side, deviating from the first connecting assembly, of the running disc, at least part of the inner wall face of the groove part is a curved surface, and the groove part is used for running of a test animal; the fixing piece is arranged at one end of the second connecting assembly away from the supporting base; the end, away from the supporting base, of the second connecting assembly is used for stretching into the area where the groove part is located so as to fix the head of a test animal on the running disc. According to the animal fixing device, the bent curved surface is arranged at the groove part of the running disc, so that stable support can be provided for the test animal when the test animal is in different body positions, stress generated when the test animal moves is counteracted, and the stability of the animal fixing device is improved.

The invention discloses blood filtration replacement liquid as well as a preparation method and application thereof. The displacement liquid comprises low molecular weight heparin calcium, prostaglandin E1, prostacyclin, electrolyte with specific concentration, glucose and an interleukin-1 receptor antagonist. The preparation method comprises the following steps: weighing the raw materials according to the proportion, dissolving to a constant volume, removing pyrogen and heat, and carrying out aseptic packaging. During clinical application, replacement liquid and blood are filtered according to the replacement rate of 1: 1-2: 1 according to the condition of a patient, 4-6 hours each time and 2-3 times each week, and related indexes are monitored. Animal tests show that the replacement liquid has good safety under the effective dosage, and has potential influence on part of organs under the high dosage. A stability test shows that the compound can be stably stored under a certain condition. The invention provides the replacement liquid which can achieve anticoagulation and blood vessel protection, maintain electrolyte balance and relieve inflammatory response, is expected to improve the prognosis of hemodialysis patients, and has important clinical significance and wide application prospects.

The utility model relates to the technical field of electric coagulation haemostatic forceps, and discloses a miniature electric coagulation haemostatic forceps for animal test surgery, which comprises two connecting rods, the left sides of the two connecting rods are fixedly connected with extension rods, the exteriors of the two extension rods are connected with sliding sleeves in a sliding manner, elastic assemblies are arranged in the extension rods, and the elastic assemblies are connected with the sliding sleeves in a sliding manner. A sliding groove is formed in the left side of the interior of the extension rod, a hemisphere is fixedly connected to the bottom of the elastic assembly, a plurality of clamping grooves are formed in the bottom side of the interior of the sliding sleeve, an outer clamp body is fixedly connected to the left side of one connecting rod, and an inner clamp body is fixedly connected to the left side of the other connecting rod. According to the haemostatic forceps, after the hemisphere moves to the designated clamping groove position, the spring releases elastic potential energy, the hemisphere is pushed to reset and be clamped in the clamping groove in the bottom of the sliding sleeve, and therefore the length of the haemostatic forceps is locked. The length of the hemostatic forceps can be flexibly adjusted according to the requirements of different operation scenes, and a doctor can operate and use the hemostatic forceps conveniently.

The invention discloses application of alkannin in preparation of drugs for resisting photodermatosis. The shikonin emulsion is used for external treatment of a constructed photoaging mouse model, and results show that after the shikonin emulsion is intervened for one week, mouse epidermis is thickened, namely, the mouse epidermis is recovered to a normal level, and after the shikonin emulsion is intervened for 14 days, mouse true dermatitis cells are also close to the normal level. When the shikonin emulsion intervenes for 7 days, cuticle pigment is reduced, the density and arrangement of dermal collagenous fibers are obviously improved, and after the shikonin emulsion intervenes for 14 days, the dermal collagenous fibers return to a normal state. The invention provides an important experimental basis for the treatment of photoaging by the shikonin emulsion through animal experiments. Furthermore, clinical tests are carried out, and the result shows that the external shikonin emulsion has a remarkable treatment effect on human actinic dermatitis clinically. Therefore, the invention provides a novel therapeutic drug and scheme for prevention and treatment of photodermatosis, and has good clinical application prospects and high clinical application values.

A therapy assembly can have an inner layer having an inner surface and an outer surface and defining a plurality of openings extending therethrough. The therapy assembly can also include a plurality of plates, each plate at least partially received within a respective opening of the plurality of openings of the inner layer. The therapy assembly can also include therapy circuitry including a cable having a plurality of electrical leads and a plurality of lead ends, each electrical lead electrically connected to a respective lead end of the plurality of lead ends. A cover layer can be attached to the outer surface of the inner layer and cover the plurality of lead ends of the cable. The plurality of lead ends can be in contact with a respective plate of the plurality of plates to define a plurality of electrodes.

The utility model discloses a wild animal test sample collection and bare-handed injection auxiliary device, and relates to the technical field of animal experiment instruments. Comprising an injector fixing barrel which is of a barrel-shaped structure provided with an injector clamping opening, and an injector barrel wing clamping piece and an injector push plate clamping piece which are matched with an injector are arranged in a clamping groove of the injector fixing barrel; the pull rod sleeve is fixedly arranged at the end part of one side, close to the injector push plate clamping piece, of the injector fixing cylinder and is connected with the injector fixing cylinder; the piston push-pull rod is movably arranged in the pull rod sleeve, and one end of the piston push-pull rod is connected with the injector push plate clamping piece; through the arrangement, the device can inject and collect urine of giant pandas under the condition that the safety distance of workers is guaranteed, the safety of the workers is effectively guaranteed, and the device is easy and convenient to use and high in practicability. The problems that in the prior art, in the production and reproduction process, collection of urine of pandas and medicine injection of the pandas cannot be effectively achieved are solved.

The utility model discloses a small animal test post-operation wound surface shooting device, which comprises a base, a support vertical rod and an object placing table, the object placing table is arranged on the upper surface of the base, the support vertical rod is vertically fixed on the base, the support vertical rod is arranged on one side of the object placing table, a support arm assembly is arranged on the support vertical rod, and the support arm assembly is connected with the object placing table. Meanwhile, a clamping head assembly is arranged on the supporting arm assembly, a shooting instrument is clamped by the clamping head assembly, a fixing table assembly is installed on the object placing table, the device achieves effective constraint on shot living animals, the scale assembly is matched with the supporting vertical rod, the supporting arm assembly and the clamping head assembly, it is ensured that the proportions of images shot by different devices at different times are consistent, and the shooting efficiency is improved. The scale is used as a correction reference, the actual area of the wound surface can be automatically converted in software, the manual measurement error is reduced, the fixing table assembly and the fixing rod are designed in a modularized mode, 20-500 g experimental animals can be compatible, and the limitation of one mouse and one device of a traditional device is overcome.

This invention belongs to the field of traditional Chinese medicine preparation, specifically disclosing a method for preparing and using a fossil-like medicinal material. This invention uses mineralized tissues such as bones, teeth, and horns of mammals like cattle, horses, deer, and sheep as raw materials, and processes them through mineral phase transformation and isostatic pressing to produce a fossil-like medicinal material. Based on the principle of natural mineralization of biological bones, the resulting fossil-like medicinal material has the same structure as natural fossil medicinal materials. Animal experiments have verified that natural dragon bone and the fossil-like medicinal material of this invention have comparable calming and sedative effects, possessing practical application value.

The present invention discloses a benzofuran compound in Eupatorium chinense and its use in treating depression. The benzofuran compound is isolated from the Chinese medicinal material Eupatorium chinense and named: 10 R -2-(10-methyl-10,11-dihydroxy)-5-acetyl-6-hydroxybenzofuran. The preparation method of the benzofuran compound uses the root of the Chinese medicine Eupatorium chinense as the raw material, and is obtained through steps of solvent extraction, activated carbon decolorization, macroporous adsorption column chromatography, and crystallization separation. This separation method is simple, effective, and has a high purity, and is applicable to industrial production. Through animal experiments on the benzofuran compound, behavioral data analysis such as the sucrose preference degree, tail suspension test, elevated plus maze test, forced swimming test, and open field test of mice is carried out. The benzofuran compound has a significant effect in treating depression, has a good efficacy in treating depression, and is expected to be developed into a new natural medicine for treating depression.

Disclosed is a cage for animal experiments, comprising: an accommodation part providing a living space for laboratory animals; a cover part coupled to close an open surface of the accommodation part; and a photographing device mounting part provided on the outer surface of the cover part, wherein the photographing device mounting part comprises a guide rail extending along one direction of the cover part, and a grip part configured to secure the photographing device and mounted such that the position thereof can be adjusted with respect to the guide rail.

The invention relates to shRNA (short hairpin Ribonucleic Acid), a recombinant expression vector, a transformant, an anti-heart failure medicine, a preparation method and pharmaceutical application thereof, and belongs to the field of biological medicine. The invention provides shRNA (short hairpin ribonucleic acid), which is selected from shRNA-AHFD-HF (short hairpin ribonucleic acid)-1 or shRNA-AHFD-HF (short hairpin ribonucleic acid)-2. The DNA (deoxyribonucleic acid) sequence corresponding to the shRNA-AHFD-HF-1 is as shown in SEQ ID NO. 1; dNA (deoxyribonucleic acid) sequences corresponding to the shRNA-AHFD-HF-2 comprise SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 and SEQ ID NO. 5. The invention also provides a recombinant expression vector based on the shRNA, a transformant, an anti-heart failure drug and a pharmaceutical application thereof, and provides a preparation method of the anti-heart failure drug. Animal experiments prove that the anti-heart-failure medicine provided by the invention can obviously improve the heart function of animals with heart failure and has an effective anti-heart-failure effect.

This invention discloses a high-pressure pneumatic rupture-type impact launcher for animal testing, belonging to the technical field of animal combat trauma simulation test equipment. The device includes a high-pressure air chamber, an air path adapter, a controllable on / off valve group, a short cavity, a diaphragm, and a diaphragm clamping and fixing structure. The high-pressure air chamber, air path adapter, and short cavity are sequentially connected. The diaphragm is installed on the end face of the short cavity through the clamping and fixing structure, forming a high-pressure ruptureable sealing structure. The device can be selectively equipped with sealing elements, a circular opening launch structure, and a square opening launch structure, enabling the simulation and preparation of various combat trauma models such as pure airflow impact, explosive shock wave, random combined injury from grenade explosion, high-speed precision projectile injury, and high-speed directional fragmentation injury. This invention features a compact structure, reliable sealing, and flexible operating condition switching. It can both reproduce the real random injury effects of the battlefield and achieve high-precision standardized injury testing, making it suitable for various animal combat trauma mechanism studies and protective effectiveness evaluations.

The invention discloses a feed additive for replacing antibiotics to treat chicken necrotic enteritis and application of the feed additive, and belongs to the technical field of feed. The effective component of the feed additive is the tannin extract from chestnut trees, and animal experiments prove that the tannin extract from chestnut trees can improve the production performance of broiler chickens with necrotic enteritis to a certain extent, improve the intestinal morphology and immune function of the broiler chickens with necrotic enteritis and improve the immunity of the broiler chickens with necrotic enteritis. Therefore, the chestnut tree tannin extract can replace antibiotics and has a good treatment effect on broiler intestinal inflammation caused by clostridium perfringens and coccidiosis.