The invention discloses an extract of artificially cultivated Phellinus phellicis fruiting bodies and its preparation method and application. The preparation method comprises: selecting the Phellinus phellinus strain Sangdu No. 1, adopting a cultivation method imitating wild bag materials under natural forests, and making the fruiting bodies Harvest when the color turns from yellow to brown or dark brown, and then obtain the fruiting body of Phellinus japonica; grind the fruiting body of Phellinus japonica to obtain powder, soak in ethanol aqueous solution, reflux extraction, concentrate the extract under reduced pressure until it has no ethanol smell, vacuum Dry to obtain the extract of the artificially cultivated Phellinus radix fruiting bodies. The extract of the invention can improve and alleviate the kidney damage of mice with hyperuricemia, increase the excretion of uric acid, and has mild action and few side effects, and can be used to prepare oral medicine and health products for preventing and treating hyperuricemia and gout.