The invention discloses a method of preparing a
taxifolin monomer from engelhardtia leaves and an application, and the method comprises the following steps: crushing or
cutting dried engelhardtia leaves into segments, adding
ethanol with a certain concentration, performing extraction, concentrating the obtained extract liquid to paste; adding a buffer with a pH of 3-6, adding a proper amount of enzymes for
enzymatic hydrolysis at a certain temperature, performing separation, enrichment, purification, and recrystallization treatment of the enzymatic
hydrolysate to obtain a
taxifolin monomer with purity of above 99%. The method of the invention is characterized in that
enzymatic hydrolysis of the engelhardtia leaf extract is directly performed to obtain the
taxifolin monomer with high purity, and the method of the invention has the advantages of strong reaction specificity, high conversion rate, simple and practical process, less
pollution, and suitability for large-scale industrial production, and the like. The disadvantages of great destructive effect on
aglycone, poor specificity, low conversion rate, high equipment corrosivity, severe
pollution, tedious process and the like in the prior art are overcome. The taxifolin monomer prepared in the invention is applicable to the prevention and treatment of
fatty liver.