71 results about "Flavolipin" patented technology

The invention discloses application of traditional Chinese medicine monomer poplar bud flavin (including homologues thereof and extracts / mixtures containing the same) in preparation of drugs for treating nerve injury diseases and a preparation method of the drugs for treating the nerve injury diseases. The pharmacological action mechanism of the monomer poplar bud flavin is deeply explored, and animal model application effect verification proves that the monomer poplar bud flavin (including homologues thereof and extracts / mixtures containing the homologues) can obviously improve core symptoms of cerebral apoplexy, autism, Alzheimer's disease and depression models, is high in safety, and can be used for preparing a medicine for treating cerebral apoplexy, autism, Alzheimer's disease and depression models. The invention also develops a medicine for treating cerebral apoplexy, autism, Alzheimer's disease and depression, which takes the poplatin as a core component (including homologues of the poplatin and extracts / mixtures containing the poplatin), and is expected to provide new research results and application schemes for treatment of nerve injury diseases such as cerebral apoplexy, autism, Alzheimer's disease, depression and the like. The important significance is realized on improving the treatment effect.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

The application discloses an evaluation method for distinguishing different quality of momordica grosvenori of different origins. The application adopts high performance liquid chromatography to establish the fingerprint of momordica grosvenori, carries out gradient elution by taking water-acetonitrile as a mobile phase, and detects the wavelength of 203 nm to obtain the HPLC fingerprint of momordica grosvenori; the obtained chromatogram is introduced into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to generate a control fingerprint, different origins of momordica grosvenori are distinguished through similarity comparison and / or chemical pattern recognition analysis; and chemical markers with VIP>1 such as 5-hydroxymethyl furfural, momordica grosvenori flavonoid, momordica grosvenori saponin V and momordica grosvenori saponin IIIA2 are screened. The application can effectively distinguish two main origins of green skin fruit and red hair fruit, overcomes the defects that traditional trait identification is subjective and it is difficult to distinguish similar appearance samples, and provides an objective, accurate and stable technical means for origin identification, quality consistency evaluation and authenticity identification of momordica grosvenori.

The invention provides a broad-spectrum combined medicine for treating colorectal cancer. The broad-spectrum combined medicine is prepared from the following components in parts by weight: 100 parts of purified water, 38 to 90 parts of tripterygium wilfordii alkaloid, 38 to 90 parts of tripterygium hypoglaucum alkaloid, 28 to 85 parts of chamaejasmine, 28 to 85 parts of gelsmium elegans alkaloid and 28 to 85 parts of xanthoxylin. The medicine is prepared from the following raw materials in parts by weight: 16 to 80 parts of ganoderan, 16 to 80 parts of pinellia ternate sitosterol, 16 to 80 parts of radix rehmanniae praeparata, 16 to 80 parts of astragaloside, 16 to 80 parts of oldenlandia diffusa alkaloid, 16 to 80 parts of sculellaria barbata alkaloid, 16 to 80 parts of tanshinone, 16 to 80 parts of carthamin, 16 to 80 parts of honeysuckle chlorogenic acid and 16 to 80 parts of pericarpium citri reticulatae flavone. By integrating anti-tumor components with multiple action mechanisms, tripterygium wilfordii alkaloid, tripterygium hypoglaucum alkaloid and the like, tumor cell proliferation can be directly inhibited, and apoptosis can be induced; tanshinone, emodin and the like can inhibit tumor angiogenesis; ganoderma lucidum polysaccharides, astragaloside and the like can activate the immune system of the body, enhance the activity of T cells and NK cells, and form double strikes of direct killing and immune surveillance.

Methods for producing organosulfur compounds from a prokaryotic cell comprising enzymes involved in the production of cysteamine, hypotaurine, and taurine. Said prokaryote may comprise a vanin selected from vanin-1 (vnn-1), vanin-2 (vnn-2), or vanin- 3 (vnn-3), a cysteamine dioxygenase (ado), and / or a flavin-containing monooxygenase 1 (fmo1). Methods include the expression of organosulfur compounds in Corynebacterium glutamicum with incubation conditions to promote compound production.

This invention relates to the field of biomedicine, and discloses the application of flavin-containing monooxygenase 2 in the preparation of drugs for the treatment of non-alcoholic fatty liver disease. This invention is the first to discover that flavin-containing monooxygenase 2 has a preventive or therapeutic effect on non-alcoholic fatty liver disease.

This invention provides an improved method for electrochemical glucose measurement and an improved composition for glucose measurement. This invention also provides a composition for glucose measurement comprising a flavin compound. This invention also provides an electrode comprising a flavin compound and a sensor and a glucose fuel cell comprising such electrode. In addition, this invention provides a method for electrochemical glucose measurement and a method for electric power generation using a flavin compound. The flavin compound of the present invention is non-toxic or less toxic and it can be thus used for a means of self-contained continuous glucose measurement.

The present invention relates to a method for producing a product using an enzyme comprising a prenylated flavin mononucleotide (prFMN) cofactor in a recombinant microorganism, wherein the recombinant microorganism is grown in a culture medium in the absence of air, and wherein the recombinant microorganism recombinantly expresses a polypeptide that facilitates the oxidative maturation of a prFMNH2 precursor into the active prFMN cofactor. Further provided are recombinant microorganism for use in such methods.

According to the folium artemisiae argyi extract, the preparation method thereof and the application of the folium artemisiae argyi extract in growth promotion, the folium artemisiae argyi extract required by a plant biostimulant comes from folium artemisiae argyi, the folium artemisiae argyi extract has the advantages of being rich in source, easy to extract and the like, green and sustainable agricultural development is facilitated, and a new scheme is provided for yield increase of crops. According to the extract, leaves and stems of traditional Chinese medicine folium artemisiae argyi serve as raw materials and are subjected to two times of ultrasonic extraction (absolute ethyl alcohol), filtrate is combined, then a crude extract is prepared, a purified component is a 60% ethanol component obtained by separating the crude extract through macroporous resin, the purified component mainly comprises a flavonoid component, and the content of eupatilin is the highest. The plant height, leaf length and fresh weight of rice, pakchoi, cotton and soybean are all improved after the fertilizer is applied according to the application mode mentioned in the invention.

The invention relates to a method for preparing irisflorentin by utilizing belamcandin, and belongs to the technical field of biology. The method comprises the following steps: carrying out C-5 site and C-3'site methylation reaction by taking white belamcandin as a substrate, taking belamcanda methyl transferase gene BcOMT03 gene encoding protein and belamcanda methyl transferase BcOMT33 gene encoding protein as catalysts and taking S-adenosine-methionine as a methyl donor to generate a corresponding glycosylation product. According to the invention, the functions of the two methyltransferase genes BcOMT03 and BcOMT33 in the blackberry lily are identified and verified for the first time, the effect of the two methyltransferase genes BcOMT03 and BcOMT33 in catalyzing the ibullamcandin to produce the irisflorentin is defined, and the blank of research on the biosynthetic pathway of the characteristic component irisflorentin of the blackberry lily is filled up; important information is provided for research on a biosynthesis mechanism of active ingredients of medicinal plants.

Provided herein are compositions and methods for measuring the levels of flavins (e.g., in biological samples). In particular, provided herein are modified biotin-riboflavin conjugates and uses thereof (e.g., to measure flavin levels).

A genetically modified prokaryotic cell to express enzymes involved in the production of taurine. Said prokaryote comprises a vanin selected from: vanin- 1 (vnn-1), vanin-2 (vnn-2), or vanin- 3 (vnn-3), a cysteamine dioxygenase (ado), and a flavin-containing monooxygenase 1 (fmol), in addition to either the addition, alteration, or deletion of at least one gene involved in taurine synthesis or transport.

The invention belongs to the technical field of Chinese wolfberry processing, and particularly relates to fresh Chinese wolfberry primary pulp rich in high-value active ingredients and a production method of the fresh Chinese wolfberry primary pulp. The method is characterized in that grinding-pulsed electric field circulation treatment is carried out, so that the retention rate of heat-sensitive active ingredients such as lycium barbarum polysaccharide, carotenoid and vitamins is increased, the flavone content is 0.41-0.45 g / 100 g, the lycium barbarum polysaccharide content is 0.54-0.57 g / 100 g, the beta-carotene content is 0.55-0.60 mg / g, the zeaxanthine content is 1.28-1.35 mg / g, the betaine content is 0.340-0.366 g / 100 g, and the linoleic acid content is 0.052-0.057 g / 100 g. The content of all nutrient elements in the Chinese wolfberry primary pulp in the prior art is far higher, and the nutrition and the efficacy value of the fresh Chinese wolfberry fruits are maximized.

Provided is a composition comprising 20-40 parts by weight of cordyceps militaris powder, 3-25 parts by weight of theaflavins, and 1.25-4.2 parts by weight of astaxanthin, which is a whitening composition or a melanin synthesis signal antagonizing composition. The composition can be used as a main ingredient in whitening food or health food.

This invention discloses a bifunctional flavin adenine dinucleotide synthase mutant and its application in FAD synthesis. The mutant disclosed in this invention is RFK / FADS. C18R or RFK / FADS C18R‑R46H ;RFK / FADS C18R The amino acid sequence is shown in SEQ ID NO.15, RFK / FADS C18R‑R46H The amino acid sequence is shown in SEQ ID NO. 16. Genetically engineered bacteria carrying the bifunctional flavin adenine dinucleotide synthase mutant gene plasmid produced flavin adenine dinucleotide using glucose as a substrate through a simple fermentation process under aerobic conditions in a shake flask. The FAD yields of strains XF02 and XF03 containing the mutant gene were 79.31 mg / L and 167.56 mg / L, respectively, representing increases of 58.62% and 235.12% compared to strain XF01 containing the non-mutated gene. By constructing microbial "cell factories" through metabolic engineering strategies, modified microorganisms can utilize inexpensive raw materials to generate desired natural products within their cells. This advancement not only reduces the production cost of FAD but also improves the environmental sustainability of its production process.

The invention discloses a preparation method of high-activity theaflavin. The method sequentially comprises the following steps: preparation and enzyme purification of tea powder, ultrasonic-assisted enzymolysis, oil-water double-liquid phase step type fermentation and online extraction, chromatographic column separation and purification, and membrane concentration and drying. According to the method, an oil-water double-liquid-phase enzymatic oxidation system is constructed, the fermentation process and the extraction process are coupled, the characteristic that the target product theaflavin-3, 3 '-digallate has a higher partition coefficient in an organic phase is utilized, in-situ separation and enrichment in the reaction process are achieved, and product degradation is avoided. In combination with a subsequently adopted specific chromatographic separation technology, the content of the high-activity TF3 monomer in the product is finally successfully increased to 50% or above of the total theaflavin monomer, and the biological activity of the product is improved.

The invention discloses an agricultural composition for preventing and treating powdery mildew of cucumbers, and belongs to the technical field of pesticides. The effective components of the agricultural composition provided by the invention consist of the physcion and the beta-lupin globulin polypeptide, and the mass ratio of the physcion to the beta-lupin globulin polypeptide is (4: 1)-(1: 8). The two components are compounded for use within the ratio range to achieve a synergistic effect.

This invention discloses an Eu-Mn-HOF material, its preparation method, and its application in the detection of flavin in Burkholderia gladioli. This invention utilizes Eu... 3+ Excitation is loaded onto a HOF support to form a red probe. Utilizing the fluorescence internal filtering effect, it becomes a turn-off fluorescent sensor, namely the Eu-Mn-HOF material. This Eu-Mn-HOF material cleverly utilizes Eu... 3+ The signal amplification function of the fluorescence internal filtration effect enables the detection method established using this Eu-Mn-HOF material to achieve an extremely low detection limit (0.839 μM), effectively meeting the needs for accurate detection and early warning of trace toxoflavones. The incubation reaction between the Eu-Mn-HOF material and toxoflavones, as well as the subsequent fluorescence detection process, only requires 15 minutes, and the entire detection cycle can be controlled within 45 minutes. This represents an efficiency improvement of more than 8 times compared to traditional detection methods, greatly satisfying the practical needs of rapid on-site food safety testing.

The invention discloses a novel application of emodin and a high-density lipoprotein analogue in preparation of a medicine. The high-density lipoprotein analogue comprises apolipoprotein A-1 single mimetic peptide and natural phospholipid. The invention has the advantages of long-term stability and no immunogenicity and organ toxicity risk, thereby providing a clinically convertible solution for precise treatment of nerve injury.

The invention relates to a recombinant microbial strain for de novo synthesis of gardenin B and application of the recombinant microbial strain in production of polymethoxylated flavone compounds, and belongs to a cross technology of synthetic biology and organic synthetic chemistry. The invention designs and constructs a heterozygous gardenin B biosynthesis pathway, and obtains a microbial strain for synthesizing gardenin B from the beginning. By screening F8H and redox partner types and regulating and controlling subcellular localization, an F8H-redox partner combination with optimal space and type is determined, and an engineering strain of which the gardenin B yield is greatly improved is obtained. The efficient synthesis method of the polymethoxylated flavonoids is established by selecting recombinant microorganisms for synthesizing gardenin B from de novo to perform co-culture fermentation or chemical conversion of fermentation products.

This invention relates to the construction and staining application of recombinant Escherichia coli for synthesizing indoleacetic acid ester. The recombinant Escherichia coli for synthesizing indoleacetic acid ester is obtained by using recombinant Escherichia coli MG1655ΔpheAΔtrpRΔtyrAΔyddGΔarsB::tac70-MaFMOΔldhA::tac70-MaFMOΔyghWX::tac70-tnaAΔpoxB::tac70-cav1 as the initial strain. Then, the endogenous acetylesterase is knocked out, and the endogenous tryptophan hydrolase gene tnaA and the flavin monooxygenase gene maFMO are overexpressed, while simultaneously overexpressing the acetyltransferase gene. The indoleacetic acid ester yield of this invention is as high as 195.3 mg / L. Using indoleacetic acid ester for staining eliminates the need for reducing agents and enzyme solutions, significantly reducing staining costs.

The invention discloses a mutant containing flavin monooxygenase and application of the mutant. According to the invention, semi-rational design and screening are carried out on the flavin-containing monooxygenase to obtain the flavin-containing monooxygenase mutant, and the amino acid sequence of the flavin-containing monooxygenase mutant is shown as SEQ ID NO.4. The mutant provided by the invention can obviously improve the activity of the flavin-containing monooxygenase and is used for improving the yield of the teer violet. By using the engineering bacteria expressing the flavin-containing monooxygenase mutant, the activity of the flavin-containing monooxygenase can be remarkably improved, the engineering bacteria are used for producing the tylosin-containing monooxygenase mutant to improve the yield, the yield of the tylosin-containing monooxygenase mutant is improved to 74.6%, and the titer of the tylosin-containing monooxygenase mutant can reach 853.7 mg L <-1 >.

The application discloses a thin layer chromatography method for isolineatin and procyanidin B2 in folium artemisiae and products thereof, and comprises the following steps: using a methanol-water solution with pH of 10-11 as an extractant to prepare a test sample solution; using methanol as a solvent to prepare a control sample solution; respectively sucking the test sample solution and the control sample solution, spotting on a thin layer plate, using dichloromethane-normal propyl alcohol-water-glacial acetic acid as a developing agent to develop, and observing under a UV lamp after air-drying. The application successfully establishes a thin layer chromatography rapid detection method for isolineatin and procyanidin B2 in folium artemisiae and products thereof, which is not only simple in operation, but also can realize semi-quantitative analysis of isolineatin and procyanidin B2, and provides a powerful detection means for quality control of folium artemisiae and products thereof.

The invention belongs to the crossing field of traditional Chinese medicine modernization and biotechnology, and particularly relates to application of a metabolic marker in preparation of a product for auxiliary diagnosis of retinal vein occlusion (RVO) or evaluation of prognosis of treatment of RVO by ginseng-wolfberry omentum granules. The metabolic marker is selected from at least one of the following metabolites: leukotriene B4, (R)-2-feruloyl-1-(4-hydroxyphenyl)-1, 2-ethylene glycol, hyoscyamine base A6, 11, 12-epoxyeicosatrienoic acid, 3, 3 ', 4, 4'-tetrahydroxy-5, 6, 7, 8, 9, 10, 11, 12-epoxyeicosatrienoic acid, 3, 3 ', 4, 4'-tetrahydroxyl-5, 6, 7, 8, 10-tetrahydroxyl-5, 7, 8, 10 The invention relates to an anti-inflammatory agent which is prepared from 5, 5 '-diisopropyl-2, 2'-dimethyl biphenyl (3, 3 ', 4, 4', 1-ethyl piperidine, flavin mononucleotide, crystal orchid glycoside, 2-O-caffeoyl arbutin, dimethyl ellagic acid glucuronide, 4 '-hydroxy-3, 4, 5-resveratrol trimethyl ether, 3'-methoxy petamicin, phenylalanyl-leucyl-isoleucyl-tyrosine and dihydroferulic acid-4-sulfate.

The invention relates to a composite nano-system, a preparation method and application, the composite nano-system is a lipid-polymer hybrid nanoparticle with a core-shell structure, and the composite nano-system comprises: a hydrophobic core which is composed of a biodegradable polymer and cholesterol and is encapsulated with a hydrophobic drug formononetin; the middle layer is composed of cation / ionized lipid and auxiliary lipid and is used for loading negatively charged anti-KRAS siRNA through electrostatic interaction to form a stable compound, and the stable compound and the hydrophobic core jointly form nanoparticles; the shell layer is composed of pegylated lipid and a targeting ligand, and the targeting ligand is tLyp-1 peptide and is covalently linked to the tail end of the pegylated lipid through a maleimide-sulfydryl click chemical reaction. The system has excellent stability, biocompatibility and tumor targeting ability, can synergistically inhibit the growth of KRAS mutation pancreatic cancer, and relates to an application of the system in preparation of drugs for treating such cancers.

To provide alternative protein-rich food products that are not derived from animals, but are free of unhealthy meat-related components such as saturated fatty acids and cholesterol, are free of the deleterious environmental effects of animal husbandry, and provide similar texture and flavor characteristics and functional properties as animal meat.SOLUTION: Structured food compositions, such as meat substitute compositions, comprising microbial-derived protein products, such as microbial-derived protein hydrolysates, are provided. Methods of making such products are also provided. Also provided are flavor potentiator compositions comprising heme-containing polypeptides, such as microbial-derived flavohemoglobin, and food compositions, such as meat substitute compositions, comprising the heme-containing flavor potentiator compositions. In one aspect, the flavor potentiator comprises a heme-containing polypeptide from a hydrogen-oxidizing microorganism, wherein (i) the hydrogen-oxidizing microorganism is a Cupriavidus microorganism or (ii) the hydrogen-oxidizing microorganism grows under microaerobic conditions.SELECTED DRAWING: None

This invention discloses the application of citrus flavonoids (NOB) or SR1078 in the preparation of drugs to improve salivary gland function, particularly in improving the circadian rhythm of salivary glands damaged by ionizing radiation. Oral administration of a specific dose of NOB or SR1078 can significantly promote salivary gland function. Furthermore, it does not interact with other drugs with anti-inflammatory or regenerative functions. It can be administered as a single dose or multiple times consecutively; it can be administered after injury, and this flexible administration method does not affect the tumor radiotherapy process.

A polypeptide comprising a residue substituted by a non-canonical amino acid (ncAA) is provided. Methods for: (a) transferring an electron to an electrode, by coupling the polypeptide to an electrode; and (b) quantifying the amount of an analyte e.g., glucose are also provided.

This application discloses flavin derivatives, their preparation methods and uses, and oxidized coenzyme NAD. + The regeneration method. Flavin derivatives are compounds comprising the structure shown in the following general formula (I): R1 and R2 are each independently selected from the group consisting of: hydrogen, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C2-C6 alkenyl; R3 and R4 are each independently selected from the group consisting of: hydrogen, C1-C6 alkyl, C1-C6 alkoxy, and C1-C6 haloalkyl; R5 is selected from the group consisting of: hydrogen, C1-C6 alkyl, phenyl, and benzyl. Chemical modification of flavin molecules, introducing small molecule groups into the side chains, can promote the conversion of NADH to NAD⁺ in solutions, cell culture systems, and animal skin tissues, regulate the intracellular NAD⁺ / NADH ratio, and has anti-aging effects, especially against skin photoaging.

The present disclosure relates to a non-natural flavin-dependent oxidase that does not comprise a disulfide bond and that is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. In some embodiments, the non-natural flavin-dependent oxidase comprises: (i) at least 70% sequence identity to SEQ ID NO: 3; and (ii) substitutions at amino acid positions Q275, C285, V323, E370, V372, N400, D404, and T438, wherein the amino acid positions correspond to SEQ ID NO:3. The present disclosure further relates to a polynucleotide, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are a composition comprising the non-natural flavin-dependent oxidase; an isolated non-natural flavin-dependent oxidase and methods of making the same; a cell extract or cell culture medium comprising the non-natural flavin-dependent oxidase; and methods of making a cannabinoid.