204 results about "Flavolipin" patented technology

The present invention relates to the construction of a high-yield indigo genetic engineering strain and a fermentation method. The present invention uses Escherichia coli as a starting strain, knocks out at least one of the yddG gene, trpR gene, tyrA gene or pheA gene in the host cell; integrates the tryptophanase gene tnaA and / or the flavin monooxygenase gene MaFMO on the genome; integrates one or more genes related to biofilm modification on the genome, and obtains the optimal engineering strain by comparing the fermentation yield; then transfers a plasmid into the strain to further strengthen the tryptophanase gene tnaA and the flavin monooxygenase gene MaFMO; finally, the yield is significantly increased by supplementing carbon sources and substrates and adding surfactants. The present invention provides an indigo-producing genetic engineering strain and a fermentation method with good stability and high synthesis efficiency, providing a reference for the subsequent development of more advantageous engineering strains.

The invention discloses a preparation method of second-generation denitrified flavin TND1128, which comprises the following steps: S1, by taking 1-methylbarbituric acid and o-aminobenzaldehyde as raw materials and a mixture of water and alcohol as a solvent, heating and reacting to generate an intermediate I; s2, taking the intermediate I as a raw material, and performing ethylation under the combined action of inorganic alkali and an ethylation reagent to generate a TND1128 crude product; s3, the TND1128 crude product is purified, and a TND1128 pure product is obtained; according to the invention, 1-methylbarbituric acid and 2-nitrobenzaldehyde are taken as raw materials for cyclization synthesis of an intermediate I, then ethylation and pulping purification are carried out under the combined action of inorganic base and an ethylation reagent, and high-purity TND1128 is synthesized in a high-yield, high-specificity and high-selectivity manner; the method has the advantages of simple process route, good reaction selectivity, high yield, cheap and easily available raw materials, low equipment requirement, easy realization of industrial production and the like.

The invention discloses a double-function flavin adenine dinucleotide synthetase mutant and application, the double-function flavin adenine dinucleotide synthetase is called RFK / FADS for short, and the amino acid sequence of the double-function flavin adenine dinucleotide synthetase is shown as SEQ ID NO.1; and the RFK / FADS mutant is RFK / FADSF55A, RFK / FADSG43Q, RFK / FADSG43Q-F55H, RFK / FADSG43Q-F55N or RFK / FADSG43Q-F55N-F129Y, and the RFK / FADSG43Q-F55N and the FADSG43Q-F55N-F129Y can be used for preparing the According to the genetically engineered bacterium disclosed by the invention, flavine adenine dinucleotide is produced through a simple fermentation process by taking glucose as a substrate under a shake flask aerobic condition, and the yields of the strains ZF02, ZF03, ZF04, ZF05 and ZF06 are respectively increased by 120%, 130%, 166%, 246% and 286% in sequence compared with those of ZF01.

The invention discloses a genetically engineered bacterium for producing tanshinol as well as a construction method and application of the genetically engineered bacterium, and the construction method comprises the following steps: (1) taking escherichia coli W3110 as an original strain, and reconstructing a tanshinol synthesis route in cytoplasm; (2) positioning flavin reductase from klebsiella pneumoniae and a 4-hydroxyphenylacetic acid-3 hydroxylase mutant in a periplasmic space by using periplasmic space positioning signal peptide sufI. The genetically engineered bacterium for producing the tanshinol disclosed by the invention has the advantages that the yield of the tanshinol reaches 2.8 g / L after being subjected to shake flask fermentation for 24 hours, and the yield of the tanshinol reaches 26.6 g / L after being fermented in a 5L fermentation tank for 48 hours.

The present disclosure relates to recombinant microorganisms, such as bacteria, e.g., E. coli, engineered to have improved flux and / or yield of the DXP pathway. Cells can be engineered to comprise nucleotide sequences comprising dxs polypeptides (EC 2.2.1.7); dxr polypeptides (EC 1.1.1.267); ispD polypeptides (EC 2.7.7.60); ispE polypeptides (EC 2.7.1.148); ispF polypeptides (EC 4.6.1.12); ispG polypeptides (EC 1.17.7.1 or EC 1.17.7.3); ispH polypeptides (EC 1.17.7.4); and idi polypeptides (EC 5.3.3.2). Cells can be further engineered to express redox polypeptides, e.g., ferredoxins, flavodoxins, and / or flavodoxin / ferredoxin--NADP reductases (EC 1.19.1.1 or EC 1.18.1.2), e.g., to increase the activity of the ispG and / or ispH polypeptides. Some or all of the nucleotide sequences can be heterologous to the cells. The recombinant microorganisms can be used in methods of isoprenoid production.

The invention discloses a bifunctional flavin adenine dinucleotide synthetase mutant and an application of the bifunctional flavin adenine dinucleotide synthetase mutant in FAD synthesis. The mutant disclosed by the invention is RFK / FADSC18R or RFK / FADSC18R-R46H, and the structural formula of the mutant is as shown in the specification. The amino acid sequence of the RFK / FADSC18R is as shown in SEQ ID NO. 15, and the amino acid sequence of the RFK / FADSC18R-R46H is as shown in SEQ ID NO. 16. A genetically engineered bacterium carrying the bifunctional flavin adenine dinucleotide synthetase mutant gene plasmid is used for producing flavin adenine dinucleotide by using glucose as a substrate through a simple fermentation process under a shake flask aerobic condition, and the FAD yields of strains XF02 and XF03 containing mutant genes are 79.31 mg / l and 167.56 mg / l in sequence; and compared with a strain XF01 containing a non-mutated gene, the mutant gene is improved by 58.62% and 235.12% respectively. A microbial'cell factory 'is constructed through a metabolic engineering strategy, and the modified microorganisms can generate required natural products in microbial cells by utilizing low-price raw materials. The progress not only reduces the production cost of the FAD, but also improves the environmental sustainability of the production process of the FAD.

The invention relates to a high-water-solubility denitroflavin covalent complex as well as a preparation method and application thereof, and belongs to the technical field of organic synthesis and medicinal chemistry. Comprising the following steps: (1) firstly, carrying out bromination reaction on 3-methyl-10-ethyl denitroflavin, so as to obtain 3-methyl-10-(1-bromoethyl) denitroflavin; and (2) coupling the 3-methyl-10-(1-bromoethyl) denitroflavin with vitamin C, so as to obtain the high-water-solubility denitroflavin covalent complex. According to the present invention, the prepared highly water-soluble denitrificin covalent complex has the two-phase solubility of the organic solvent and the water, such that the water solubility of the 3-methyl-10-ethyl-denitrificin is substantially enhanced, and the application range of the denitrificin is expanded. And meanwhile, the denitrified flavin covalent compound also has good antioxidant activity, and the antioxidant duration can be prolonged.

The invention discloses a bacteriostatic composition which comprises a synergist and a bacteriostatic agent, the synergist is ethyl phenylacetate or a derivative thereof, and the bacteriostatic agent is selected from hydrogen peroxide, beta-lactam antibiotics, macrolide antibiotics and aminoglycoside antibiotics. The synergist disclosed by the invention is wide in synergistic effect spectrum range, and has a remarkable synergistic bacteriostatic effect when being combined with hydrogen peroxide, amoxicillin, ampicillin, benzylpenicillin potassium, erythromycin, azithromycin and amikacin; the antibacterial effect is weak when the compound is independently used, but the virulence of staphylococcus aureus can be remarkably reduced, the formation of hemolysin, staphylococcus flavin, enterotoxin and a biofilm can be inhibited, and meanwhile, the selective pressure on bacteria is favorably reduced due to the relatively low antibacterial activity, so that the occurrence of drug resistance is delayed; the method is suitable for various application scenes such as food processing industry.

The invention relates to the technical field of microorganisms, in particular to monascus for high-yield production of monascus and / or monascus flavin and application of the monascus, a new monascus strain with the preservation number of CGMCC NO.41335 and application of the new monascus strain in production of high-content monascus and / or monascus flavin and related products thereof. The strain is monascus purpureus ZYXQ-2206, can produce high-efficiency and non-toxic monascus and / or monascus flavin at high yield, and does not produce harmful and toxic components such as lovastatin and citrinin. Therefore, a monascus product produced by using the strain can effectively avoid safety risks, and has extremely high application prospects and market potential. By screening and optimizing a fermentation process, the invention provides the high-yield, non-toxic and high-functionality red yeast rice, and the red yeast rice has important application value in the field of development of lipid-lowering functional foods and nutritional health-care products.

The invention relates to an apolipoprotein E detection reagent and application thereof, and belongs to the technical field of biomedicine. The invention provides application of a plant derivative in preparation of an APOE epitope exposure agent. The plant derivative is selected from at least one of curcumin, emodin, EF24 and FLLL-32. The invention also provides an APOE epitope exposure agent which comprises a surfactant, salts, a reducing agent and plant derivatives. According to the present invention, the sample and the protein are pre-treated by using the epitope exposure agent containing the plant derivative to improve the sufficiency of the ApoE protein epitope exposure so as to achieve the accurate typing of the ApoE gene based on the immunological detection method and the accurate quantification of the proteins with different genotypes. Genetic typing of ApoE and quantitative detection of proteins with different genotypes are carried out based on an immunological detection method, and the genotype detection result is highly consistent with a fluorescent PCR (Polymerase Chain Reaction) result.

The invention discloses application of a polymer coupled with a flavin group to catalysis of conversion of NADH (nicotinamide adenine dinucleotide) in a reduction state into NAD + (nicotinamide adenine dinucleotide) in an oxidation state, which is characterized in that in a solution containing various components, the polymer coupled with the flavin group catalyzes the NADH to convert the NAD +, the conversion rate is 0.007-0.85 mu M / s, and the conversion efficiency is 20-100%; the polymer coupled with the flavin group is a product obtained by coupling flavin with a polymer containing a cationic group side group through a covalent bond, the molar ratio of the flavin group in a product molecule to the cationic group in the polymer is 1: 1-1: 100, and the polymer is composed of a repetitive unit containing the cationic group and other repetitive units. Wherein the molar fraction of the amino-containing repeating unit is 5%-100%. Compared with riboflavin, flavin mononucleotide and other biomolecules containing flavin groups, the activity of catalyzing NADH to be oxidized into NAD + can be improved by at least 50 times or above.

The invention relates to construction and dyeing application of recombinant Escherichia coli for synthesizing indole acetate, which is characterized in that recombinant Escherichia coli MG1655 delta pheA delta trpR delta tyrA delta yddG delta arsB: tac 70-MaFMO delta ldhA: tac 70-MaFMO delta yghWX: tac 70-tnaA delta poxB: tac 70-cav1 is taken as an initial strain, then endogenous acetylesterase is knocked out, and the recombinant Escherichia coli for synthesizing indole acetate is obtained by the steps of: preparing the recombinant Escherichia coli for synthesizing indole acetate. An endogenous tryptophan hydrolase gene tnaA and a flavin-containing monooxygenase gene maFMO are overexpressed, and meanwhile, an acetyltransferase gene is overexpressed, so that the tryptophan-containing flavin-containing monooxygenase gene is obtained; the yield of the indole acetate reaches up to 195.3 mg / L. When the indole acetate is used for dyeing, a reducing agent and an enzyme solution are not used, so that the dyeing cost is greatly reduced.

The invention belongs to the technical field of natural plant extracts, and particularly relates to a preparation method, a product and application of an antioxidant repair rosemary extract. According to the technical scheme, snail protease and bromelain are added during rosemary leaf pretreatment, so that cell walls of rosemary leaves are fully destroyed, and release of intracellular active ingredients can be effectively promoted. According to the technical scheme, an extraction solvent A and an extraction solvent B are used, the extraction solvent A is mainly used for extracting flavonoid substances such as rutin, hesperetin and thistle flavin in the rosemary, and the extraction solvent B is mainly used for extracting terpene components such as rosmarinic acid, carnosic acid and rosmarinol in the rosemary. According to the technical scheme, the rosemary extract is obtained by mixing and extracting the extract A and the extract B, and it is found through experimental verification that the extract has remarkable effects in the aspects of oxidation resistance, soothing and skin repairing.

The invention belongs to the technical field of medicines, and particularly provides a novel application of a theaflavin compound in preparation of a product for inhibiting dipeptidyl peptidase-4 (DPP-4), and the theaflavin compound is one or more of theaflavin-3-gallate (TF2A), theaflavin-3 '-digallate (TF3) and theaflavin (TF). The research systematically proves that TF2A, TF3 and TF have new application of inhibiting DPP-4 for the first time: in-vitro enzymology and fluorescence experiments show that theaflavin can inhibit DPP-4 and interact with DPP-4, and molecular docking further shows possible binding configuration and key action sites of theaflavin. In vivo, the activity of DPP-4 in serum and different tissues of high-fat diet mice can be reduced, serum glucagon-like peptide-1 (GLP-1) can be up-regulated, and glycometabolism and inflammatory phenotype can be improved. Therefore, the theaflavin compound disclosed by the invention can be applied to preparation of products for inhibiting DPP-4, and has a good development prospect.

The invention discloses a processing method of radix astragali stir-fried with honey and bran. The method comprises the following steps: refining honey, preparing wheat bran, preparing honey and bran and preparing radix astragali stir-fried with honey and bran. Through the optimized processing technology designed by the invention, the quality of radix astragali processed products is remarkably improved, and chemical components contained in the radix astragali processed products are reserved to the maximum extent, so that the safety, effectiveness and controllability of clinical medication of the radix astragali processed products are ensured. According to the radix astragali fried with honey and bran, the beany flavor is obviously weakened, so that the taste is improved, and the total content of the symbolic component astragaloside of the radix astragali and the five flavonoid components calycosin-7-glucoside, the mediodarin, the formononin, the formononetin and the calycosin-7-glucoside of the radix astragali is increased compared with that of the radix astragali. The invention revives the processing technology of honey-bran-fried astragalus membranaceus which is endangered to be lost, protects and excavates the special processing method of genuine medicinal materials in Gansu province, and can ensure that the special traditional Chinese medicine processing variety of honey-bran-fried astragalus membranaceus can be propagated in generations.

The invention discloses a method for directionally preparing indigo blue or indirubin by biocatalytically converting indoleglycoside fermentation liquor, which is characterized in that beta-glucosidase Asbg1 and flavin-dependent monooxygenase MaFMO are combined, and the indoleglycoside fermentation liquor is used as a substrate to directionally prepare the indigo blue or indirubin. A glucose dehydrogenase (GDH)-mediated NADPH circulating system is introduced as a process supply cofactor, and then process conditions are optimized and regulated, so that a process for directionally preparing the indigo blue or indirubin by taking the indole glycoside fermentation liquor as a substrate is established, and the directional preparation of the indigo blue or indirubin is realized. The process provides a method for realizing green and efficient directional preparation of indigo blue or indirubin.

The invention discloses application of traditional Chinese medicine monomer poplar bud flavin (including homologues thereof and extracts / mixtures containing the same) in preparation of drugs for treating nerve injury diseases and a preparation method of the drugs for treating the nerve injury diseases. The pharmacological action mechanism of the monomer poplar bud flavin is deeply explored, and animal model application effect verification proves that the monomer poplar bud flavin (including homologues thereof and extracts / mixtures containing the homologues) can obviously improve core symptoms of cerebral apoplexy, autism, Alzheimer's disease and depression models, is high in safety, and can be used for preparing a medicine for treating cerebral apoplexy, autism, Alzheimer's disease and depression models. The invention also develops a medicine for treating cerebral apoplexy, autism, Alzheimer's disease and depression, which takes the poplatin as a core component (including homologues of the poplatin and extracts / mixtures containing the poplatin), and is expected to provide new research results and application schemes for treatment of nerve injury diseases such as cerebral apoplexy, autism, Alzheimer's disease, depression and the like. The important significance is realized on improving the treatment effect.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

The invention discloses application of ginkgetin in treatment of chronic pulmonary obstruction, and relates to the field of medical technical methods of ginkgetin. According to the invention, by constructing a model of cigarette extract on airway epithelial cell injury, the biflavone compound is found to have a protective effect on epithelial cell injury caused by smoking; through transcriptomics research, biflavone compounds such as ginkgetin and the like are found to have pharmacological activity of inhibiting CEBP beta protein high expression caused by cigarette extracts, so that the effect of protecting airway inflammation caused by smoking is achieved. More importantly, the ginkgetin and the glucocorticoid are jointly used, so that the treatment effect on chronic pulmonary obstruction is remarkably improved compared with that of independently using the ginkgetin and the glucocorticoid, and the ginkgetin and the glucocorticoid have wide medical application.

The application discloses an evaluation method for distinguishing different quality of momordica grosvenori of different origins. The application adopts high performance liquid chromatography to establish the fingerprint of momordica grosvenori, carries out gradient elution by taking water-acetonitrile as a mobile phase, and detects the wavelength of 203 nm to obtain the HPLC fingerprint of momordica grosvenori; the obtained chromatogram is introduced into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to generate a control fingerprint, different origins of momordica grosvenori are distinguished through similarity comparison and / or chemical pattern recognition analysis; and chemical markers with VIP>1 such as 5-hydroxymethyl furfural, momordica grosvenori flavonoid, momordica grosvenori saponin V and momordica grosvenori saponin IIIA2 are screened. The application can effectively distinguish two main origins of green skin fruit and red hair fruit, overcomes the defects that traditional trait identification is subjective and it is difficult to distinguish similar appearance samples, and provides an objective, accurate and stable technical means for origin identification, quality consistency evaluation and authenticity identification of momordica grosvenori.

The invention provides a broad-spectrum combined medicine for treating colorectal cancer. The broad-spectrum combined medicine is prepared from the following components in parts by weight: 100 parts of purified water, 38 to 90 parts of tripterygium wilfordii alkaloid, 38 to 90 parts of tripterygium hypoglaucum alkaloid, 28 to 85 parts of chamaejasmine, 28 to 85 parts of gelsmium elegans alkaloid and 28 to 85 parts of xanthoxylin. The medicine is prepared from the following raw materials in parts by weight: 16 to 80 parts of ganoderan, 16 to 80 parts of pinellia ternate sitosterol, 16 to 80 parts of radix rehmanniae praeparata, 16 to 80 parts of astragaloside, 16 to 80 parts of oldenlandia diffusa alkaloid, 16 to 80 parts of sculellaria barbata alkaloid, 16 to 80 parts of tanshinone, 16 to 80 parts of carthamin, 16 to 80 parts of honeysuckle chlorogenic acid and 16 to 80 parts of pericarpium citri reticulatae flavone. By integrating anti-tumor components with multiple action mechanisms, tripterygium wilfordii alkaloid, tripterygium hypoglaucum alkaloid and the like, tumor cell proliferation can be directly inhibited, and apoptosis can be induced; tanshinone, emodin and the like can inhibit tumor angiogenesis; ganoderma lucidum polysaccharides, astragaloside and the like can activate the immune system of the body, enhance the activity of T cells and NK cells, and form double strikes of direct killing and immune surveillance.

Methods for producing organosulfur compounds from a prokaryotic cell comprising enzymes involved in the production of cysteamine, hypotaurine, and taurine. Said prokaryote may comprise a vanin selected from vanin-1 (vnn-1), vanin-2 (vnn-2), or vanin- 3 (vnn-3), a cysteamine dioxygenase (ado), and / or a flavin-containing monooxygenase 1 (fmo1). Methods include the expression of organosulfur compounds in Corynebacterium glutamicum with incubation conditions to promote compound production.

This invention relates to the field of biomedicine, and discloses the application of flavin-containing monooxygenase 2 in the preparation of drugs for the treatment of non-alcoholic fatty liver disease. This invention is the first to discover that flavin-containing monooxygenase 2 has a preventive or therapeutic effect on non-alcoholic fatty liver disease.

This invention provides an improved method for electrochemical glucose measurement and an improved composition for glucose measurement. This invention also provides a composition for glucose measurement comprising a flavin compound. This invention also provides an electrode comprising a flavin compound and a sensor and a glucose fuel cell comprising such electrode. In addition, this invention provides a method for electrochemical glucose measurement and a method for electric power generation using a flavin compound. The flavin compound of the present invention is non-toxic or less toxic and it can be thus used for a means of self-contained continuous glucose measurement.

The present invention relates to a method for producing a product using an enzyme comprising a prenylated flavin mononucleotide (prFMN) cofactor in a recombinant microorganism, wherein the recombinant microorganism is grown in a culture medium in the absence of air, and wherein the recombinant microorganism recombinantly expresses a polypeptide that facilitates the oxidative maturation of a prFMNH2 precursor into the active prFMN cofactor. Further provided are recombinant microorganism for use in such methods.

The present invention relates to a treatment of cancer. Here, the inventors discovered that flavin-containing monooxygenase 4 (FM04) expression was increased in lung tumor samples compared with lung healthy tissue. They found that this phenotype was a common feature in lung adenocarcinoma promoted also by translocation of EML4 / ALK as well as by oncogenic KRAS or EGFR using dedicated mouse models. Moreover, FMO4 was strongly increased in human lung tumors compared with healthy lung tissue, and FMO4 expression was inversely correlated with overall survival in patients with lung adenocarcinoma. Importantly, FMO4 protected lung cancer cells against reactive oxidative species-induced apoptosis. Finally, FMO4 loss of function decreased oncogenic KRAS-induced human bronchoalveolar cells transformation in vitro and in vivo. Thus, the present invention relates to an FMO inhibitor for use in the treatment of cancer in a subject in need thereof.

The invention discloses application of vitexin (the molecular formula of the vitexin is C19H18O8, the molecular weight of the vitexin is 374.34, and the CAS number of the vitexin is 479-91-4) in preparation of antitumor drugs. The method comprises the following steps: firstly, treating esophageal cancer cell lines KYSE70 and KYSE450 with vitexin, detecting cell proliferation conditions after 0, 24 hours, 48 hours and 72 hours, and determining that the vitexin has an inhibiting effect on the esophageal cancer cell lines; the vitexin can inhibit cell proliferation of skin cancer cell lines SK-MEL-5 and SK-MEL-28 and ovarian cancer cell lines SKOV3 and TOV112D under the same concentration, and compared with a control group, the growth speed of tumor cells is remarkably reduced along with increase of the concentration of the vitexin, so that the vitexin has a remarkable effect on treating esophageal cancer, skin cancer and ovarian cancer; secondly, through a Pull down experiment, it is found that vitexin targets PRDX1, and it is indicated that vitexin can inhibit proliferation of tumor cells in vivo and in vitro by targeting PRDX1.

The present invention relates to a fluorescent inspection liquid which is highly safe to a human body and emits blue fluorescence having high visibility in the leakage inspection. The fluorescent inspection liquid contains water as a solvent and two blue fluorescent dyes selected from a flavin derivative, quinine, pyrene and anthocyanin. The flavin derivative is a derivative having a flavin skeleton and obtained from a starting material, riboflavin.

According to the folium artemisiae argyi extract, the preparation method thereof and the application of the folium artemisiae argyi extract in growth promotion, the folium artemisiae argyi extract required by a plant biostimulant comes from folium artemisiae argyi, the folium artemisiae argyi extract has the advantages of being rich in source, easy to extract and the like, green and sustainable agricultural development is facilitated, and a new scheme is provided for yield increase of crops. According to the extract, leaves and stems of traditional Chinese medicine folium artemisiae argyi serve as raw materials and are subjected to two times of ultrasonic extraction (absolute ethyl alcohol), filtrate is combined, then a crude extract is prepared, a purified component is a 60% ethanol component obtained by separating the crude extract through macroporous resin, the purified component mainly comprises a flavonoid component, and the content of eupatilin is the highest. The plant height, leaf length and fresh weight of rice, pakchoi, cotton and soybean are all improved after the fertilizer is applied according to the application mode mentioned in the invention.

The invention relates to a method for preparing irisflorentin by utilizing belamcandin, and belongs to the technical field of biology. The method comprises the following steps: carrying out C-5 site and C-3'site methylation reaction by taking white belamcandin as a substrate, taking belamcanda methyl transferase gene BcOMT03 gene encoding protein and belamcanda methyl transferase BcOMT33 gene encoding protein as catalysts and taking S-adenosine-methionine as a methyl donor to generate a corresponding glycosylation product. According to the invention, the functions of the two methyltransferase genes BcOMT03 and BcOMT33 in the blackberry lily are identified and verified for the first time, the effect of the two methyltransferase genes BcOMT03 and BcOMT33 in catalyzing the ibullamcandin to produce the irisflorentin is defined, and the blank of research on the biosynthetic pathway of the characteristic component irisflorentin of the blackberry lily is filled up; important information is provided for research on a biosynthesis mechanism of active ingredients of medicinal plants.