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79 results about "Flavin adenine dinucleotide" patented technology
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In biochemistry, flavin adenine dinucleotide (FAD) is a redox-active coenzyme associated with various proteins, which is involved with several important enzymatic reactions in metabolism. A flavoprotein is a protein that contains a flavin group, this may be in the form of FAD or flavin mononucleotide (FMN). There are many flavoproteins besides components of the succinate dehydrogenase complex, including α-ketoglutarate dehydrogenase and a component of the pyruvate dehydrogenase complex; some examples are shown in section 6.
Modified flavin adenine dinucleotide dependent glucose dehydrogenase
ActiveUS20080220460A1Reduce heat-inactivationDecreasing amount of usedFungiBacteriaFlavin adenine dinucleotideGlucose sensors
Object: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.Method for Achieving the Object: A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.
Owner:TOYO TOYOBO CO LTD
Use of Endogenous Fluorescence to Identify Invading Metastatic Breast Tumor Cells
ActiveUS20080212867A1Accurate representationEliminate needDiagnostics using lightCharacter and pattern recognitionFlavin adenine dinucleotideAbnormal tissue growth
The present invention broadly provides methods and systems for detecting, identifying, and characterizing conditions, including diseases and other disorders in human or other animal subjects, by analyzing fluorescence from endogenous flavin adenine dinucleotide (FAD) fluorophors present in biological materials and samples. In particular embodiments, the invention relates to conditions of the human breast including cancers such as carcinoma. Methods and systems are provided for detecting, locating, and characterizing tumors and precancerous tissue via nonlinear optical imaging techniques capable of accurately characterizing fluorescence intensities and fluorescent lifetime parameters from endogenous FAD fluorophors present in a test tissue sample.
Owner:WISCONSIN ALUMNI RES FOUND
Recombinant human NADH (nicotinamide-adenine dinucleotide) dehydrogenase subunit-4 gene and constructing method of expression vector thereof
The invention discloses a recombinant human NADH (nicotinamide-adenine dinucleotid) dehydrogenase subunit-4 gene and a constructing method of an expression vector thereof. The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the size of the nucleotide sequence is 2889 bp. The constructing steps of adeno-associate virus vectors are as follows: firstly constructing a recombinant adeno-associated virus vector containing the human NADH dehydrogenase subunit-4 gene, and then coating, infecting, purifying, concentrating and identifying the recombinant adeno-associated virus. According to the method, the recombinant adeno-associated virus vector with the recombinant human NADH dehydrogenase subunit-4 gene can be quickly and simply constructed, and is packaged to obtain the adeno-associated virus vector with complex defects. The gene can be used for gene therapy of LHON (leber's hereditary optic neuropathy).
Owner:WUHAN NEUROPHTH BIOTECHNOLOGY LTD CO
Use of thiamine and nicotine adenine dinucleotide for butanol production
The invention relates generally to the field of industrial microbiology and alcohol production. More specifically, the invention relates to the use of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) to improve butanol production. Butanol production can be improved by providing sufficient amounts of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) in the production media.
Owner:GEVO INC
Recombinant human NADH (nicotinamide-adenine dinucleotide) dehydrogenase subunit-4 gene and constructing method of expression vector thereof
Owner:WUHAN NEUROPHTH BIOTECHNOLOGY LTD CO
Use of endogenous fluorescence to identify invading metastatic breast tumor cells
ActiveUS8144966B2Accurate representationEliminate needDiagnostics using lightCharacter and pattern recognitionAbnormal tissue growthFlavin adenine dinucleotide
The present invention broadly provides methods and systems for detecting, identifying, and characterizing conditions, including diseases and other disorders in human or other animal subjects, by analyzing fluorescence from endogenous flavin adenine dinucleotide (FAD) fluorophors present in biological materials and samples. In particular embodiments, the invention relates to conditions of the human breast including cancers such as carcinoma. Methods and systems are provided for detecting, locating, and characterizing tumors and precancerous tissue via nonlinear optical imaging techniques capable of accurately characterizing fluorescence intensities and fluorescent lifetime parameters from endogenous FAD fluorophors present in a test tissue sample.
Owner:WISCONSIN ALUMNI RES FOUND
Modified flavin adenine dinucleotide dependent glucose dehydrogenase
ActiveUS20090317848A1Decreasing amount of usedReduce the heat-inactivation of enzymesBioreactor/fermenter combinationsBiological substance pretreatmentsFlavin adenine dinucleotideGlucose sensors
Owner:TOYOBO CO LTD
Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
ActiveUS20120171708A1Improved temperature dependenceAccurate measurementImmobilised enzymesBioreactor/fermenter combinationsFlavin adenine dinucleotideBlood sugar
Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent.A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO: 2 or an amino acid sequence that has a 60% homology therewith, and that has an improved temperature dependency.
Owner:TOYO TOYOBO CO LTD
Method Of Determining The Viability Of At Least One Cell
ActiveUS20140363840A1Microbiological testing/measurementBiological material analysisFlavin adenine dinucleotidePhosphate
This disclosure provides a method of determining the viability of at least one cell via quantification of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), flavin adenine dinucleotide (FAD), and / or collagen. The method includes the steps of contacting the at least one cell with light having a wavelength of from 700 to 900 nm, using two photon excitation, or from 335 to 400 nm, using one photon excitation, to induce an optical response from the NAD(P)H, FAD, and / or collagen and measuring the optical response. The method also includes the step of quantifying one or more of an amount, spatial localization, and / or time-dependent response of the NAD(P)H, FAD, and / or collagen utilizing the optical response.
Owner:RGT UNIV OF MICHIGAN
Novel glucose dehydrogenase
ActiveUS20160265021A1Improve thermal stabilityReduce riskMicrobiological testing/measurementMaterial analysis by electric/magnetic meansGlucose dehydrogenase activityFlavin adenine dinucleotide
Provided is a flavin adenine dinucleotide-dependent glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 78% or more identity to the amino acid sequence of SEQ ID NO: 3, and having glucose dehydrogenase activity.
Owner:TOYOBO CO LTD
Fermentation technology of 3Ec nano enzyme
Owner:EIN INTL
Biosynthetic gene cluster of ikarugamycin and application of biosynthetic gene cluster
The invention discloses a biosynthetic gene cluster of ikarugamycin and an application of the biosynthetic gene cluster. The nucleotide sequence of the biosynthetic gene cluster of the ikarugamycin is as shown in the 3411th to 15678th sites of SEQ ID NO. 1 and contains three genes, namely a heterozygous polyketone / non-ribosomal polypeptide synthetase gene ikaA, FAD (Flavin Adenine Dinucleotide) dependent oxidoreductase gene ikaB and similar Michael cyclization reaction enzyme gene ikaC. All the genes and protein information relevant to the biosynthesis of the ikarugamycin are helpful to interpret the biosynthesis mechanism of natural products of a polycyclic tetramate large-ring lactam family so as to provide a theoretical basis and materials for further genetic modification. The genes and proteins can be used for digging or creating compounds or genes and proteins applicable to the medicine and health industry and industry and agriculture in nature.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Stable compound coenzyme preparation as well as preparation method and applications thereof
InactiveCN104623626AGood repeatabilityReduce lossOrganic active ingredientsPowder deliveryFlavin adenine dinucleotideDisease
The invention provides a stable compound coenzyme preparation and a preparation method thereof. The main compositions and proportions of the stable compound coenzyme preparation are as follows: 90-120 U of coenzyme A (CoA), 0.1-0.3 mg / ml coenzyme I (NAD), 1-5 mg / ml glutathione (GSH), 1-2.5 mg / ml adenosine triphosphate (ATP), 1.8-12 mu g / ml flavin mononucleotide (FMN), 3-13 mu g / ml flavin adenine dinucleotide (FAD), 0.1-0.4 mg / ml adenosine diphosphate (ADP), 0.2-0.4 mg / ml adenosine monophosphate (AMP), 1.2-15 mu g / ml of adenosine methionine (SAM), 1-5 mg / ml calcium gluconate, 0.5-1.5 mg / ml cysteine hydrochloride, and 0.6-5 mg / ml mannitol. The preparation method overcomes the defects that high-speed centrifugalization is adopted, freeze-drying conditions are not clear and the product stability is poor in the existing method, and the stable compound coenzyme preparation is widely applied to the practices of preparing drugs for treating cardia-cerebrovascular diseases and digestive system diseases.
Owner:BEIJING SL PHARMA +2
Use of thiamine and nicotine adenine dinucleotide for butanol production
The invention relates generally to the field of industrial microbiology and alcohol production. More specifically, the invention relates to the use of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) to improve butanol production. Butanol production can be improved by providing sufficient amounts of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) in the production media.
Owner:GEVO INC
Enzyme composition and use therefore
InactiveUS20100151554A1Improve combustion efficiencyTransferasesOxidoreductasesFlavin adenine dinucleotideThiamine pyrophosphate
The present invention provides an enzyme composition and a method for treating coal using the enzyme composition. The enzyme composition includes at least one enzyme, coenzyme and ammonium acetate, wherein the enzyme can be laccase-isozyme, pyruvate dehydrogenase, dihydrolipoyl transacetylase, and dihydrolipoyl dehydrogenase, and the coenzyme can be CoA, CoA-SH, thiamine pyrophosphate, lipoic acid, flavin adenine dinucleotide, and nicotinamide adenine dinucleotide. In addition, the method of the present invention includes treating the coal with the enzyme composition for more than 72 hours.
Owner:EIN INTL
Carbon dioxide measurement kit
InactiveCN103558370AEasy to useEasy to operateBiological testingFlavin adenine dinucleotidePhosphoenolpyruvate carboxylase
The invention provides a carbon dioxide measurement kit. The carbon dioxide measurement kit mainly comprises the following components: 50 to 200mM of a buffer solution, 10 to 50% of a stabilizer, 0.1 to 5% of a surface active agent, 0.1 to 5g / L of a preservative, 1 to 20mM of a reaction accelerator, 2 to 10mM of 3-acetylpyridine adenine dinucleotide (reduction type), 5 to 50g / L of phosphoenolpyruvic acid, 50 to 500U / L of phosphoenolpyruvate carboxylase, and 1 to 3KU / L of malate dehydrogenase; a recycling system of 3-acetylpyridine adenine dinucleotide (reduction type) comprises (1) 2 to 20g / L of glucose substrate, (2) 100 to 2,000U / L of corresponding glucose dehydrogenase, and (3) 1 to 5mM of 3-acetylpyridine adenine dinucleotide. The carbon dioxide measurement kit is convenient to use and simple to operate; the components participating in coupling reaction are extra added, so that no pollution caused by endogenous and allogenic substances are introduced; the rate of the recycling system is below the main reaction rate, thus the main reaction cannot be interfered; the carbon dioxide measurement kit is high in stability and can be used for a long time.
Owner:QINGDAO JINYANG BIOTECH
Biosensor based on glucose dehydrogenase and detecting method
InactiveCN107064261ASolving the difficulty of accepting electrons from flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenaseGood response sensitivityMaterial analysis by electric/magnetic meansFlavin adenine dinucleotideDichlorophenolindophenol
The invention relates to the field of a biological detection instrument, and particularly relates to a biosensor based on glucose dehydrogenase. The biosensor is composed of an insulation substrate, an electrode system, a detecting reagent, a middle interlayer, a covering layer, a reaction room and a sample injection channel; the reaction room is provided with air holes. Enzyme in the detection reagent is flavin adenine dinucleotide dependent form glucose dehydrogenase; the electronic medium is commonly formed by ruthenium complex and 2, 6-dichlorophen indophenol. The invention further provides a detecting method of the biosensor. The biosensor solves the problem that the pure ruthenium complex is very hard to accept electronic from the flavin adenine dinucleotide dependent form glucose dehydrogenase, thus the biosensor has the advantages of good reaction sensitivity and linearity.
Owner:TAIZHOU E LINKCARE MEDITECH CO LTD
Protein having flavin adenine dinucleotide-dependent glucose dehydrogenase activity
InactiveUS20150267178A1Improve thermal stabilityMaintain good propertiesAnimal cellsBacteriaFlavin adenine dinucleotideGlucose dehydrogenase activity
The present invention provides a protein derived from a thermophilic filamentous fungus having flavin adenine dinucleotide-dependent glucose dehydrogenase activity. The present invention provides an enzyme for glucose measurement that has better thermal stability than that of conventional enzymes while being unaffected by dissolved oxygen, not requiring the addition of a coenzyme, and maintaining the excellent properties of FADGDH in terms of having excellent substrate specificity (particularly low reactivity with maltose).
Owner:NAT INST OF ADVANCED IND SCI & TECH
Flavin adenine dinucleotide-binding glucose dehydrogenase
The object is to provide a novel enzyme which enables to determine the glucose level more accurately, a bacterium capable of producing the enzyme, and use of the enzyme. Disclosed is a flavin adenine dinucleotide-binding glucose dehydrogenase having the following properties (1) to (3):(1) the enzyme has an activity of catalyzing a reaction for oxidizing a hydroxyl group in glucose in the presence of an electron acceptor to produce glucono-delta-lactone; (2) the enzyme has a molecular weight of about 100 kDa as measured by SDS-polyacrylamide gel electrophoresis or about 400 kDa as measured by gel filtration chromatography; and (3) the enzyme is less reactive to maltose, D-fructose, D-mannose and D-galactose. Also disclosed is a microorganism Aspergillus oryzae which can produce the enzyme. Further disclosed are a glucose determination method, a reagent for the determination of glucose, and a kit for the determination of glucose, each utilizing the enzyme.
Owner:AMANO ENZYME INC
Method of detecting thalassemia by optical analysis of blood components
ActiveUS20140332697A1Readily apparentPhotometryLuminescent dosimetersFlavin adenine dinucleotideBlood component
The method of detecting thalassemia by optical analysis of blood components is a spectral detection method that is based on the fluorescence spectra of a set of biomolecules, including tyrosine, tryptophan, nicotinamide adenine dinucleotide, and flavin adenine dinucleotide, which are all found in blood plasma, and porphyrin, which is found in red blood cells (RBCs). Measured ratios of intensity maxima between tryptophan and nicotinamide adenine dinucleotide, flavin adenine dinucleotide and nicotinamide adenine dinucleotide, tyrosine and tryptophan, and the normal form of porphyrin and the basic form of porphyrin may each be used, alone or in combination, to diagnose a patient as suffering from thalassemia.
Owner:KING SAUD UNIVERSITY
Triglyceride determination kit and determination method thereof
ActiveCN108949903AImprove measurement accuracyImprove stabilityMicrobiological testing/measurementMethylanilineFlavin adenine dinucleotide
The invention provides a triglyceride determination kit. The kit comprises a liquid single reagent R1, the reagent R1 includes the following components with concentration: lipoprotein esterase, glycerol kinase, glycerol phosphate oxidase, peroxidase, piperazine-1,4-diethanesulfonic acid, NaOH, 4-aminoantipyrine, adenosine triphosphate, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium orN-ethyl-N-(2-hydroxy-3-propyl)-3'5-dimethoxyaniline sodium salt, sodium glutamate, magnesium acetate, flavin adenine dinucleotide, polyethylene glycol, and a betaine solution. The kit belongs to the technical field of biological detection, and the triglyceride determination kit provided by the invention significantly enhances the anti-interference ability while improving the stability of the reagent, and has good measurement accuracy for triglyceride.
Owner:广州市伊川生物科技有限公司
Cholesterol oxidase affinity matrix, synthesis and large-scale purification method thereof for cholesterol oxidase
ActiveCN102380347AEfficient purificationImprove purification efficiencyOther chemical processesOxidoreductasesFlavin adenine dinucleotideBenzene
The invention relates to a cholesterol oxidase affinity matrix formed by linking a cholesterol oxidase affinity ligand and a chromatography media; more preferably, the cholesterol oxidase affinity ligand is FAD (Flavin Adenine Dinucleotide) segmental riboflavin and the chromatography media is Sepharose, chitosan or polyvinyl benzene; the invention further provides a synthesis method for cholesterol oxidase affinity matrix, and a large-scale purification method for cholesterol oxidase by using the cholesterol oxidase affinity matrix; the cholesterol oxidase can be rapidly separated and purified in large scale by using the cholesterol oxidase affinity matrix provided by the invention; on the condition of only using one step of affinity chromatography, the pure cholesterol oxidase is obtained; the consumption time of the entire purification step is short, the active recovery rate of protein is higher and the large-scale popularization and application is applicable.
Owner:宁夏妙朗生物科技有限公司
Serum high-density liptein cholesterol test kit
ActiveCN111534568ANo reconstitution requiredImprove stabilityMicrobiological testing/measurementMethylanilineAmpyrone
The invention provides a high-density liptein cholesterol test kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains the following components: 3-morpholinopropanesulfonic acid (MOPS) buffer solution, alpha-cyclic glucan sulfate, dextran sulfate, pulullan, magnesium chloride, N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-sodium methylaniline, a surface active agent, and a preservative; and the reagent R2 contains the following components: 3-morpholinopropanesulfonic acid (MOPS) buffer solution, cholesterol esterase (CEH), cholesterol oxidase (COD), peroxidase (POD), 4-ampyrone,flavin adenine dinucleotide disodium, a surface active agent, a stabilizer, and a preservative. The invention also provides a preparation method and application of the kit. The kit is a liquid kit, which is high in stability, anti-interference capability and accuracy, and good in repeatability.
Owner:中拓生物有限公司 +2
L-glutamate oxidase
The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties:(A) action: catalyzing the following reaction:L-glutamic acid+O2+H2O→α-ketoglutaric acid+H2O2+NH3;(B) substrate specificity: being specific to L-glutamic acid;(C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000±6,000, molecular weight as determined through gel filtration of 140,000±10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000±6,000;(D) optimum pH: around pH 6.0 to 8.5;(E) heat stability: being stable up to 60° C. at a pH of 7.4 for 30 minutes; and(F) coenzyme: flavin adenine dinucleotide (FAD).
Owner:YAMASA SHOYU CO LTD
Method for improving aureomycin yield as well as recombinant expression vector and genetic engineering bacterium of aureomycin
ActiveCN103205481AIncrease productionRaise the ratioBacteriaMicroorganism based processesFlavin adenine dinucleotideStreptomyces auratus
The invention discloses a method for improving aureomycin yield as well as a recombinant expression vector and a genetic engineering bacterium of the aureomycin. The recombinant expression vector is constructed by utilizing an FADH2(Flavin Adenine Dinucleotide) dependent chloride enzyme gene ctcP and an FAD reductase gene ctcQ; the recombinant expression vector is transferred to streptomycesaureofaciens to obtain the genetic engineering bacterium of the high-yield aureomycin; and the bottleneck of chlorination in an aureomycin synthetic route is broken by over-expression of the FADH2 dependent chloride enzyme gene ctcP and the FAD reductase gene ctcQ of the obtained engineering strain, so that the yield of the aureomycin in streptomyces aureus is improved. The strain is obtained for laying a foundation for improving the aureomycin yield and reducing the production cost of the aureomycin.
Owner:金河生物科技股份有限公司 +1
Glucose sensor
ActiveUS8658011B2Increased substrate specificityReduced responseImmobilised enzymesBioreactor/fermenter combinationsFlavin adenine dinucleotideGlucose sensors
Provided is a glucose sensor that is capable of measuring a glucose concentration even in the case where Aspergillus oryzae type FAD-GDH (flavin adenine dinucleotide-glucose dehyrogenase) and a ruthenium compound are used in combination. The glucose sensor includes an insulative substrate, an electrode system having a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system, wherein the reagent layer contains Aspergillus oryzae type FAD-GDH, a ruthenium compound, and PMS (phenazine methosulfate).
Owner:ARKRAY INC
Glycosylated modified flavin adenine dinucleotide-dependent glucose dehydrogenases, compositions thereof as well as methods of making and using the same
ActiveUS20150064733A1Improve temperature stabilitySugar derivativesMicroorganismsFlavin adenine dinucleotideAmino acid substitution
Compositions, devices, kits and methods are disclosed for assaying glucose with a glycosylated, modified flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), variant thereof or an active fragment thereof, where at least one asparagine residue at positions N2, N168 and N346 of mature, wild-type A. oryzae FAD-GDH according to SEQ ID NO:2 is substituted by one or more amino acids not suitable for glycosylation, thereby eliminating or inactivating, respectively, a potential glycosylation site at this position.
Owner:ROCHE DIABETES CARE INC
Matrix Stability Compositions, Test Elements, Test Systems and Methods of Use Thereof
ActiveUS20150177178A1Improve test stabilityReduce adsorptionImmobilised enzymesBioreactor/fermenter combinationsFlavin adenine dinucleotideSpecific test
Reagent matrices and methods are disclosed for improving the stability of test elements with respect to degradation by humidity in the air. The reagent matrices and methods are based upon using an effective amount of a deliquescent material to decrease the sorption of water from the air by other components of a reagent matrix. When the test element is a glucose-specific test strip, and the reagent matrix can include a glucose dehydrogenase enzyme and / or a flavin adenine dinucleotide cofactor, a nitrosoaniline mediator, and a film former, where the deliquescent material may include a salt such as sodium chloride in an amount effective to absorb water from the atmosphere at a rate that is faster than the rate at which the other components of the reagent matrix absorb water from the atmosphere.
Owner:ROCHE DIABETES CARE INC
Flavin adenine dinucleotide disodium salt freeze-dried powder needle preparation and preparation thereof
The invention relates to a FAD-Na2 freeze-dried acanthopanax powder spasmolytic preparation and a method for preparing the same. The FAD-Na2 freeze-dried acanthopanax powder spasmolytic preparation is a drug composition made by mixing FAD-Na2 serving as medicinal active ingredients and pharmaceutically acceptable excipients. The preparation method is as follows: the FAD-Na2 is used as raw material and added with certain spices of excipients according to certain proportions; the mixture is prepared and developed by a technical means provided by the invention into freeze-dried acanthopanax powder spasmolytic agent which can be used for subcutaneous injection, intramuscular and intravenous injection and used to cure a variety of diseases caused by lacks of vitamin B2.
Owner:BEIJING RUNDEKANG MEDICAL TECH CO LTD
Production and purification method of glucose dehydrogenase by taking FAD (Flavin Adenine Dinucleotide) as prothetic group
InactiveCN108486027AGood substrate specificityImprove thermal stabilityBacteriaMicroorganism based processesEscherichia coliFlavin adenine dinucleotide
The invention discloses a production and purification method of glucose dehydrogenase by taking FAD (Flavin Adenine Dinucleotide) as a prothetic group, and belongs to the field of bioengineering. Theproduction and purification method is characterized by expressing the glucose dehydrogenase sourced from burkholderia cepacia by taking Escherichia coli as a host, carrying out batch feeding, controlling the temperature in stages, and expressing the glucose dehydrogenase. According to the production and purification method disclosed by the invention, the activity of the glucose dehydrogenase is upto 22200.0U / L, and the mycelia content is up to 69.8g / L; a crude enzyme solution is separated and purified through three-step chromatography, so that recombinase of which the specific enzyme activityis 10<9>U / mg, the property problem of enzyme is solved, and the recombinase is suitable for industrial production.
Owner:JIANGNAN UNIV
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