33results about How to "No reconstitution required" patented technology

The invention provides a glutathione reductase assay kit, which comprises a reagent R1 and a reagent R2. The reagent R1 includes: 50-200 mmol / L of a Tris buffer solution, 0.5-2 mmol / L of EDTA, 1-5 kU / L of pyruvic carboxylase, 1-5 kU / L of ascorbic acid oxidase, 5-20 mg / L of potassium ferrocyanide, a surfactant and a preservative; the reagent R2 includes: 50-200 mmol / L of the Tris buffer solution, 0.5-2 mmol / L of EDTA, 2-10 mmol / L of GSSG, 0.1-0.5 mmol / L of NADPH, 1-15 g / L of a stabilizer, and 0.5-2 g / L of a preservative. The liquid assay kit has good stability and is strong in anti-interferenceeffect. The invention also discloses a preparation method and an application of the assay kit.

The invention discloses a glycated serum protein determination kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains Tris buffer solution, trypsin, peroxidase, an anti-interference substance, a protein denaturant, an ionic liquid, CaCl2, a surfactant and a preservative; and the reagent 2 contains the Tris buffer solution, fructosaminase, 4-aminoantipyrine, sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate (TOOS), a surfactant, a stabilizer and a preservative. The kit disclosed by the invention is a liquid kit high in accuracy and good in stability. The invention also discloses a preparation method and application of the kit.

The invention discloses a kit for measuring apolipoprotein C2. The kit contains a reagent R1, a reagent R2 and a calibration product, wherein the reagent R1 is prepared from: a buffer solution, electrolyte, a surfactant, a reaction enhancer, a stabilizer and a preservative; the reagent R2 is prepared from: a buffer solution, goat anti-human apolipoprotein C2 antibody latex particles, electrolyte,a surfactant, a stabilizer and a preservative; and the calibration product is prepared from: a buffer solution, recombinant apolipoprotein C2, bovine serum albumin, mannitol and sodium azide. The apolipoprotein C2 kit adopts double liquid reagents, redissolving preparation is not needed, and the kit can be directly used after a bottle is opened. The obtained latex particles are good in stability,and the accuracy of the detecting result is greatly improved; residue glycosyl at the Fc terminal of the apolipoprotein C2 antibody is oxidized into a formyl group through the sodium periodate, the binding rate of the antibody is increased, the stability of the antibody is improved, an active area where the antibody and antigen are bonded is effectively protected, and the sensitivity and stabilityof the detecting reagents are improved.

The present invention provides a pyruvic acid determination reagent box. The reagent box comprises a reagent R1 and a reagent R2. The reagent R1 comprises the following ingredients: reduced coenzyme I(NADH), a trihydroxymethyl aminomethane (Tris) buffer solution, a surface active agent, EDTA-Na2 and a preservative. The reagent R2 comprises the following ingredients: the trihydroxymethyl aminomethane (Tris) buffer solution, lactate dehydrogenase (LDH), the surface active agent, a stabilizer and the preservative. The present also provides a preparation method of the reagent box and applications. The reagent box is a high-stability, high-accuracy and good-repeatability liquid reagent box.

The invention discloses a glutathione peroxidase determination kit, the kit contains a reagent R1 and a reagent R2; the reagent R1 contains buffer solution, EDTA, glutathione reductase, reduced glutathione, potassium chloride, magnesium chloride, surfactant, stabilizer and preservative; and the reagent R2 contains the buffer solution, the EDTA, NADPH, cumene hydrogen peroxide, surfactant, stabilizer and preservative. The invention also discloses a preparation method of the glutathione peroxidase determination kit and application of the glutathione peroxidase determination kit. The kit providedby the invention is a high-sensitivity and excellently stable liquid kit.

The invention provides a high-density liptein cholesterol test kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains the following components: 3-morpholinopropanesulfonic acid (MOPS) buffer solution, alpha-cyclic glucan sulfate, dextran sulfate, pulullan, magnesium chloride, N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-sodium methylaniline, a surface active agent, and a preservative; and the reagent R2 contains the following components: 3-morpholinopropanesulfonic acid (MOPS) buffer solution, cholesterol esterase (CEH), cholesterol oxidase (COD), peroxidase (POD), 4-ampyrone,flavin adenine dinucleotide disodium, a surface active agent, a stabilizer, and a preservative. The invention also provides a preparation method and application of the kit. The kit is a liquid kit, which is high in stability, anti-interference capability and accuracy, and good in repeatability.

The invention provides an apolipoprotein A1 (ApoA1) determination kit, which contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a 4-hydroxyethylpiperazineethanesulfonic acid (HEPES) buffer solution, NaCl, guanidine hydrochloride, sodium decyl sulfate, tetramethylurea, polyethylene glycol 6000, a surfactant and a preservative, and the reagent R2 is prepared from a 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, goat anti-human ApoA1 antibody coated latex particles, a surfactant, a stabilizer and a preservative. The invention also provides a preparation method and application of the kit, wherein the kit is beneficial to exposure of antigen sites in lipoprotein and promotion of antigen-antibody reaction due to mutualsynergy of multiple components, and is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

The invention relates to the field of quality control of medical examination, in particular to the preparation of a single quality control substance of a hepatitis B e antigen. The hepatitis B virus eantigen is diluted to a required value by using a specific stabilizer to prepare the quality control product. The preparation process is simple, the production cost is low, added substances and contents are controllable, the product belongs to a liquid type, redissolution is not required, and the composition is stable. Through a biological traceability link program and reference method assignmentof the program, the product can be used as a third-party medical laboratory quality control product during the validity period to meet the needs of clinical infectious disease testing.

The invention provides an angiotensin converting enzyme assay kit, the kit contains a reagent R1 and a reagent R2, the reagent R1 comprises the following components: N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, sodium chloride, an ion activator, polyglycerol fatty acid ester, 4-hydroxy 2, 2, 6, 6-tetramethyl-1-oxo-piperidine (AAQ-2) and a preservative; and the reagent R2 is preparedfrom the following components: N-tri (hydroxymethyl) methyl-3-aminopropanesulfonic acid, FAPGG, a stabilizer and a preservative. The invention also provides a preparation method and application of thekit. The kit is a liquid kit with strong anti-interference capability, strong stability and good repeatability.

The invention discloses a serum alpha 1-acid glycoprotein measurement kit, comprising a reagent R1, a reagent R2 and a calibration product, wherein the reagent R1 is composed of a buffer solution, sodium chloride and polyethylene glycol coated magnetic nano-particles; the reagent R2 is composed of a buffer solution, a goat anti-human alpha 1-acid glycoprotein antibody, a surfactant, a stabilizer and a preservative; the calibration product is composed of a buffer solution, an alpha 1-acid glycoprotein antigen, bovine serum albumin, mannitol and sodium azide. The invention further discloses an application of the kit. The alpha 1-acid glycoprotein is a liquid double-reagent, which can be directly used by opening the bottle without reconstitution. By adding the polyethylene glycol coated magnetic nano-particles to the reagent R1, the stability of the magnetic nano-particles is improved, and the antibody forms agglomeration on the magnetic nano-particles, thereby greatly improving the analytical sensitivity of the reagent. By adding the bovine serum albumin and glycerol to the reagent R2, the stability of the reagent is improved.

The invention discloses a serum iron determination kit. The kit comprises a reagent R1 and a reagent R2. The reagent R1 comprises a buffer solution, ascorbic acid, a de-interfering agent, a stabilizer, a surfactant, and a preservative. The reagent R2 comprises a buffer solution and bathophenanthroline disulfonic acid disodium salt. The invention further relates to a preparation method and application of the serum iron determination kit. The stable serum iron is a liquid double-reagent, reconstitution preparation is not required, and therefore the stable serum iron can be directly used when taken out from a bottle. The stabilizer is added in the reagent R1 to facilitate stability and reducibility maintenance of the ascorbic acid. The de-interfering agent is added in the reagent R1, thioureais mixed with a ascorbic acid solution, and therefore the R1 can be adopted as a masking agent as well as a reducing agent, and interference from other ions can be avoided. The reagent has high sensitivity. The kit is convenient to use, is high in specificity, is high in accuracy, is free of pollution to biochemical instruments, and can be further popularized in the market.

The invention provides an apolipoprotein B (ApoB) assay kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, NaCl, guanidine hydrochloride, sodium decyl sulfate, tetramethylurea, polyethylene glycol 6000, a surfactant and a preservative; and the reagent R2 is prepared from the 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, goat anti-human ApoB antibody coated latex particles, the surfactant, a stabilizer and the preservative. The invention also provides a preparation method and an application of the kit, and the kit is beneficial to exposure of antigen sites in lipoprotein and promotion of an antigen-antibody reaction due to mutualsynergy of multiple components, and is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

The present invention provides a pyruvic acid determination reagent box. The reagent box comprises a reagent R1 and a reagent R2. The reagent R1 comprises the following ingredients: reduced coenzyme I(NADH), a trihydroxymethyl aminomethane (Tris) buffer solution, a surface active agent, EDTA-Na2 and a preservative. The reagent R2 comprises the following ingredients: the trihydroxymethyl aminomethane (Tris) buffer solution, lactate dehydrogenase (LDH), the surface active agent, a stabilizer and the preservative. The present also provides a preparation method of the reagent box and applications. The reagent box is a high-stability, high-accuracy and good-repeatability liquid reagent box.

The invention discloses a glycated serum protein determination kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains Tris buffer solution, trypsin, peroxidase, an anti-interference substance, a protein denaturant, an ionic liquid, CaCl2, a surfactant and a preservative; and the reagent 2 contains the Tris buffer solution, fructosaminase, 4-aminoantipyrine, sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate (TOOS), a surfactant, a stabilizer and a preservative. The kit disclosed by the invention is a liquid kit high in accuracy and good in stability. The invention also discloses a preparation method and application of the kit.

The invention relates to the technical field of biological detection, in particular to a preparation method of a liquid calibrator of a fructosamine determination kit. The method comprises the following steps: (1) saccharifying: dissolving animal serum albumin in a 0.05-2 mol / L phosphate buffer solution until the concentration of the animal serum albumin reaches 10-60 g / L, adding glucose until the concentration of the glucose reaches 1.25-7.5 g / L, stirring for 6-10 hours at the speed of 800-2000 r / min under the water bath condition of 40-70 DEG C, and placing in a refrigerator for 5-24 hours to obtain a reagent solution A; (2) removing glucose; and (3) sub-packaging. The method is high in safety, low in raw material consumption, high in yield and low in time consumption, residual glucose is removed by using a biological method, freeze-drying and dialysis are not needed, and the long-term stability is good when the method is stored at 2-8 DEG C.

The invention provides a pepsinogen II (PGII) assay kit, the kit contains reagent R1 and reagent R2; reagent R1 contains the following components: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, NaCl , sodium hydroxide, surfactants, preservatives. Reagent R2: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, mouse anti-human PGII antibody coated latex particles, suspending agent, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit can effectively avoid the interference of chylomicrons, and adopts different kinds of protective agents and suspending agents at the same time. It is a kind of kit with strong stability, high sensitivity and good repeatability. Low cost liquid kit.

The invention provides an angiotensin converting enzyme assay kit, the kit contains a reagent R1 and a reagent R2, the reagent R1 comprises the following components: N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, sodium chloride, an ion activator, polyglycerol fatty acid ester, 4-hydroxy 2, 2, 6, 6-tetramethyl-1-oxo-piperidine (AAQ-2) and a preservative; and the reagent R2 is preparedfrom the following components: N-tri (hydroxymethyl) methyl-3-aminopropanesulfonic acid, FAPGG, a stabilizer and a preservative. The invention also provides a preparation method and application of thekit. The kit is a liquid kit with strong anti-interference capability, strong stability and good repeatability.

The invention provides a pepsinogen I (PGI) determination kit. The kit contains a reagent R1 and a reagent R2, the reagent R1 contains the following components: a piperazine 1, 4-diethylsulfonic acid(PIPES) buffer solution, NaCl, sodium hydroxide, a surfactant and a preservative, and the reagent R2 comprises the piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, mouse anti-human PGI antibody coated latex particles, a suspending aid, a stabilizer and the preservative. The invention also provides a preparation method and application of the kit. The kit can effectively avoid the interference of chyle particles, different types of protective agents and suspending agents are adopted at the same time, and the kit is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

The invention provides an alpha fetoprotein variant 3 (AFP-L3) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components: a piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, NaCl, a surfactant, a suspending aid and a preservative; and the reagent R2 is prepared from the piperazine-1, 4-diethylsulfonic acid (PIPES) buffersolution, goat anti-human AFP-L3 antibody coated latex particles, goat anti-human AFP-L3 antibody coated magnetic nanoparticles, the suspending aid, a stabilizer and the preservative. The invention also provides a preparation method and an application of the kit, and the kit can effectively replace a chemiluminescence immunoassay analyzer kit, is a novel liquid kit with strong stability, high sensitivity, good repeatability and low cost, and is convenient for clinical development and application of projects.

The invention provides a direct bilirubin (DB) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components: a phosphate buffer solution, NaCl, Nacetylcysteine, sodium fluoride, disodium ethylene diamine tetraacetate, ascorbic acid oxidase, a surfactant and a preservative; the reagent R2 comprises a trihydroxymethyl aminomethane hydrochloric acid buffer solution, bilirubin oxidase, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit adopts different types of stabilizers and surfactants, interference of blood fat can be effectively avoided; the liquid kit is high in interference resistance, high in sensitivity, good in stability and low in cost.

The invention provides a direct bilirubin (DB) assay kit, the kit contains reagent R1 and reagent R2; the reagent R1 contains the following components: phosphate buffer, NaCl, N-acetylcysteine, sodium fluoride , Disodium EDTA, Ascorbate Oxidase, Surfactant, Preservative. Reagent R2: tris-hydrochloric acid buffer, bilirubin oxidase, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit uses different types of stabilizers and surfactants, which can effectively avoid the interference of blood lipids. It has strong anti-interference ability, high sensitivity and good stability. Low cost liquid kit.

The invention provides a total bilirubin (TB) assay kit, the kit contains reagent R1 and reagent R2; reagent R1 contains the following components: tris-hydrochloric acid buffer, NaCl, sodium cholate, lemon Acids, surfactants, preservatives. Reagent R2: tris-hydrochloric acid buffer, bilirubin oxidase, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit uses different types of stabilizers and surfactants, which can effectively avoid the interference of blood lipids. It has strong anti-interference ability, high sensitivity and good stability. Low cost liquid kit.

The invention provides a procalcitonin (PCT) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, NaCl, MgCl2, chondroitin sulfate, hyaluronic acid, a surfactant and a preservative. And the reagent R2 is prepared from piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, goat anti-human PCT antibody coated latex particles, a suspending aid, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit can effectively avoid interference of chyle particles, latex particles of different specifications, novel surfactants and different protective agents and suspending agents are adoptedat the same time, and the kit is a liquid kit which is high in stability, high in sensitivity, good in repeatability and low in cost.