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Apolipoprotein C2 measuring kit with high sensitivity

An apolipoprotein and kit technology, used in biological testing, measuring devices, material testing products, etc., can solve the problems of low specificity, affecting the sensitivity and stability of reagents, interference, etc., to achieve good stability, improve sensitivity and The effect of stability and protection of active area

Inactive Publication Date: 2019-04-12
中拓生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two commonly used methods for preparing latex-antibody protein complexes, physical adsorption method and chemical coupling method. The physical adsorption method will cause partial analysis and structural changes of latex microsphere surface proteins. The prepared latex-antibody protein complexes The specificity is low, it is easily interfered by other substances (such as rheumatoid factor and heterophile antibody), and its application in biological diagnosis is limited, while the chemical coupling method can control the problems in the adsorption to the greatest extent.
The chemical coupling method to link the Fab fragment and Fc of the antibody is random, which affects the sensitivity and stability of the reagent

Method used

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  • Apolipoprotein C2 measuring kit with high sensitivity
  • Apolipoprotein C2 measuring kit with high sensitivity
  • Apolipoprotein C2 measuring kit with high sensitivity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A kit for measuring apolipoprotein C2 with high sensitivity and good stability, which includes reagent R1, reagent R2 and calibrator.

[0062] The reagent R1 consists of:

[0063] Phosphate buffer 150mmol / L

[0064] Sodium chloride 120mmol / L

[0065] Triton 100 1 g / L

[0066] PEG6000 50g / L

[0067] Trehalose 1 g / L

[0068] Sodium azide 1 g / L

[0069] pH 7.5

[0070] Reagent R2 consists of:

[0071] Phosphate buffer 120mmol / L

[0072] Goat anti-human apolipoprotein C2 antibody latex particles 30ml / L

[0073] Sodium chloride 130mmol / L

[0074] Triton 100 1 g / L

[0075] Bovine serum albumin 1 g / L

[0076] Sodium azide 1 g / L

[0077] pH 7.5

[0078] The calibrator consisted of:

[0079] Phosphate buffer 120mmol / L

[0080] Recombinant apolipoprotein C2 6mg / dl

[0081] Bovine serum albumin 1g / L

[0082] Mannitol 1g / L

[0083] Sodium azide 1g / L

[0084] pH 8.0

[0085] The preparation method of the above-mentioned apolipoprotein C2 assay kit comprises the f...

Embodiment 2

[0091] The reagent R1 consists of:

[0092] Phosphate buffer 160mmol / L

[0093] Sodium chloride 100mmol / L

[0094] Brij-35 1 g / L

[0095] PEG6000 50g / L

[0096] Trehalose 1 g / L

[0097] Sodium azide 1 g / L

[0098] pH 7.5

[0099] Reagent R2 consists of:

[0100] Phosphate buffer 100mmol / L

[0101] Goat anti-human apolipoprotein C2 antibody latex particles 30ml / L

[0102] Magnesium chloride 120mmol / L

[0103] Brij-35 1 g / L

[0104] Propylene glycol 1 g / L

[0105] Sodium azide 1 g / L

[0106] pH 7.5

[0107] The calibrator consisted of:

[0108] Phosphate buffer 160mmol / L

[0109] Recombinant apolipoprotein C2 7mg / dl

[0110] Bovine serum albumin 1g / L

[0111] Mannitol 1g / L

[0112] Sodium azide 1g / L

[0113] pH 8.0

[0114] The preparation method is the same as in Example 1.

Embodiment 3

[0116] HEPES buffer 160mmol / L

[0117] Sodium chloride 130mmol / L

[0118] Brij-35 1 g / L

[0119] PEG6000 50g / L

[0120] Trehalose 1 g / L

[0121] Sodium azide 1 g / L

[0122] pH 7.5

[0123] Reagent R2 consists of:

[0124] HEPES buffer 120mmol / L

[0125] Goat anti-human apolipoprotein C2 antibody latex particles 35ml / L

[0126] Magnesium chloride 130mmol / L

[0127] Brij-35 1 g / L

[0128] Propylene glycol 1 g / L

[0129] Sodium azide 1 g / L

[0130] pH 7.5

[0131] The calibrator consisted of:

[0132] HEPES buffer 160mmol / L

[0133] Recombinant protein C2 7mg / dl

[0134] Bovine serum albumin 1g / L

[0135] Mannitol 1g / L

[0136] Sodium azide 1g / L

[0137] pH 8.0

[0138] The preparation method is the same as in Example 1.

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Abstract

The invention discloses a kit for measuring apolipoprotein C2. The kit contains a reagent R1, a reagent R2 and a calibration product, wherein the reagent R1 is prepared from: a buffer solution, electrolyte, a surfactant, a reaction enhancer, a stabilizer and a preservative; the reagent R2 is prepared from: a buffer solution, goat anti-human apolipoprotein C2 antibody latex particles, electrolyte,a surfactant, a stabilizer and a preservative; and the calibration product is prepared from: a buffer solution, recombinant apolipoprotein C2, bovine serum albumin, mannitol and sodium azide. The apolipoprotein C2 kit adopts double liquid reagents, redissolving preparation is not needed, and the kit can be directly used after a bottle is opened. The obtained latex particles are good in stability,and the accuracy of the detecting result is greatly improved; residue glycosyl at the Fc terminal of the apolipoprotein C2 antibody is oxidized into a formyl group through the sodium periodate, the binding rate of the antibody is increased, the stability of the antibody is improved, an active area where the antibody and antigen are bonded is effectively protected, and the sensitivity and stabilityof the detecting reagents are improved.

Description

technical field [0001] The invention relates to the field of medical immunological in vitro diagnostic reagents, in particular to a kit for measuring apolipoprotein C2 by latex immunoturbidimetry. Background technique [0002] Apolipoprotein is a huge family, including A, B, C, E and other families. Apolipoprotein is a special kind of protein. In the process of lipid transport, it must be combined with apolipoprotein before it can be transported normally. [0003] Human Apo C2 is a single polypeptide chain containing 79 amino acid residues with a molecular weight of 9.1kD. The first synthesized ApoC2 contains 101 amino acid residues, of which 22 amino acids constitute the signal peptide, which is converted into mature ApoC2 after the signal peptide is removed. The isoelectric point of ApoC2 is 5.0, and its amino acid composition has three characteristics: (1) lack of histidine and cysteine; (2) more polar amino acids; (3) higher threonine and serine . [0004] Lipoprotein...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6803
Inventor 王飞刘安娜张强
Owner 中拓生物有限公司
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