18 results about "Immunoturbidimetry" patented technology

The invention discloses a kit for determining oxidized low-density lipoprotein based on latex enhanced immunoturbidimetry and application, and belongs to the technical field of biological detection reagents, the kit comprises a reaction buffer solution and a latex reagent, the reaction buffer solution is marked as R1, and the latex reagent is marked as R2; the reaction buffer solution comprises 3-10 g / L of a buffer solution, 5-15 g / L of inorganic salt, 10-30 g / L of a turbidity increasing agent, 0.5-2.5 mL / L of a surfactant, 1-3 mL / L of a preservative and 3-14 g / L of a stabilizer; the latex reagents are at least two latex reagents formed by coupling monoclonal antibodies and latex microspheres, and the particle size range of the latex microspheres is 200-280 nm. The latex is marked by adopting two monoclonal antibodies with relatively strong specificity, so that the specificity is relatively strong under the condition of keeping the sensitivity. According to the kit, the detection sensitivity and specificity can be improved at the same time, so that the linear range of the kit is wider, the accuracy is higher, and clinical requirements are better met.

There is a need to quantify both low and high concentrations of target substances with high sensitivity. [Solution] The present invention provides an immunoturbidimetric reagent comprising a first particle and a second particle, wherein the ratio of the volume-average particle size (Dv2) of the second particle to the volume-average particle size (Dv1) of the first particle, Dv2 / Dv1, is 0.37 or more and 0.86 or less, the volume-average particle size of the second particle is 150 nm or more, and the ratio of the refractive index (R2) of the second particle to the refractive index (R1) of the first particle, R2 / R1, is 0.87 or more and 0.97 or less.

The invention belongs to the technical field of biological detection, and particularly relates to a detection method and a detection kit for oxidized low-density lipoprotein. The invention provides a novel application of quaternary ammonium salt chitosan in a kit, and particularly relates to application of quaternary ammonium salt chitosan with different molecular weights in an immunoturbidimetry detection process of oxidized low-density lipoprotein. When high-molecular-weight quaternized chitosan with the molecular weight of 100-200 kDa and low-molecular-weight quaternized chitosan with the molecular weight of 30-50 kDa are used as components of the oxidized low-density lipoprotein detection kit, the quaternized chitosan with different molecular weights can react with citrate radicals to form gel particles with different mechanical strengths; the content of the oxidized low-density lipoprotein can be detected through immunoturbidimetry, and the sensitivity and the linear range of the oxidized low-density lipoprotein detection kit are greatly improved and widened by using the quaternized chitosan with different molecular weights.

The application provides a lipoprotein phospholipase A2 determination kit for latex immunoturbidimetry, which contains reagent R1 and reagent R2; the reagent R1 comprises the following components: buffer, inorganic salt, anti-interference additive, surfactant and Proclin 300; the reagent R2 comprises the following components: buffer, lipoprotein phospholipase A2 antibody coated latex microspheres, stabilizer, surfactant and Proclin 300. The kit is a liquid kit with strong stability, high accuracy, wide linear range and strong anti-HOOK capability.

The invention discloses a serum COVID-19 NAb quantitative detection reagent as well as preparation and application thereof, and belongs to the technical field of biomedical detection. The latex microsphere reagent contained in the quantitative detection reagent is produced by adopting a continuous process, so that complicated purification processes such as centrifugation and washing are avoided, the preparation time is remarkably shortened, and the complexity of the production process and the difference between batches are reduced. Meanwhile, by optimizing a buffer solution system and reaction conditions, the detection sensitivity and the linear range are improved, the linear range is 122.46 ng / mL to 10000 ng / mL, the correlation coefficient R2 is equal to 0.9999, the absolute relative deviation does not exceed + / -1.88%, the detection requirements of neutralizing antibodies (NAb) with different concentrations in clinical samples can be met, and the defect that the linear range of a traditional method is narrow is overcome. The reagent is based on an immunoturbidimetry principle, the detection process can be carried out on a full-automatic biochemical analyzer, the operation is simple and convenient, and the detection is efficient.

The invention discloses a reagent, a kit and a detection method for free light chain detection, and relates to the technical field of detection kits, the reagent comprises the following components: a solid phase carrier surface marked with an F (ab ') 2 antibody fragment; the liquid-phase reaction liquid comprises a composite blocking agent for inhibiting non-specific reaction of endogenous interferents; wherein the F (ab ') 2 antibody fragment and the composite blocking agent act together to improve the detection specificity and reaction linearity. The problems that non-specific reaction is caused by an antibody Fc segment and an endogenous interferent when a latex enhanced immunoturbidimetric assay is used for detecting free light chains, existing means cannot eradicate the non-specific reaction, detection linearity is abnormal, and specificity is low are solved, the solid phase carrier marked with the F (ab ') 2 antibody segment and the composite blocking agent are used, and the solid phase carrier marked with the F (ab') 2 antibody segment and the composite blocking agent have a synergistic effect; closed-loop protection is formed from two dimensions of eliminating self interference of the antibody and shielding exogenous interference of the sample, and compared with an antibody optimization or interference shielding means, the combination targeting and detection specificity of a target analyte are synergistically improved.

This invention belongs to the field of bioassay technology, and specifically relates to a method and kit for detecting oxidized low-density lipoprotein (ODL). This invention provides a novel application of quaternized chitosan in the kit, specifically using quaternized chitosan of different molecular weights in the immunoturbidimetric detection of ODL. Results show that when high molecular weight quaternized chitosan (100-200 kDa) and low molecular weight quaternized chitosan (30-50 kDa) are used as components of the ODL detection kit, the different molecular weights of quaternized chitosan react with citrate to form gel particles of varying mechanical strengths. This allows for the detection of ODL content via immunoturbidimetry. Furthermore, the use of quaternized chitosan of different molecular weights significantly improves the sensitivity and linear range of the ODL detection kit.

This invention relates to the field of immunoassay technology, specifically to a kit, preparation method, and detection method for determining p2PSA based on latex-enhanced immunoturbidimetric assay. The kit includes: a reaction solution and a latex antibody reagent; the reaction solution includes at least one of a buffer, inorganic salt, sensitizer, surfactant, blocking agent, and preservative; the latex antibody reagent includes a mixture of a first latex antibody reagent and a second latex antibody reagent; the first latex antibody reagent is formed by coupling a polyclonal antibody of p2PSA with large-particle latex microspheres; the particle size range of the large-particle latex microspheres is 172–405 nm; the second latex antibody reagent is formed by coupling a monoclonal antibody of p2PSA with small-particle latex microspheres; the particle size range of the small-particle latex microspheres is 71–121 nm; the monoclonal antibody of p2PSA includes three different monoclonal antibodies of p2PSA; the kit of this invention can ensure sensitivity in determining p2PSA while broadening the linearity and enhancing specificity.

The application relates to a free light chain, a quality control product and application thereof, the free light chain comprising one or both of a free kappa light chain and a free lambda light chain; wherein the free kappa light chain has an amino acid sequence as shown in SEQ ID NO. 1 or an amino acid sequence with at least 90 percent identity thereof; and the free lambda light chain has an amino acid sequence as shown in SEQ ID NO. 2 or an amino acid sequence with at least 90 percent identity thereof. The quality control product prepared by using the free light chain of the application can be used for quality control of various detection methods such as immunofixation electrophoresis, immunoturbidimetry and mass spectrometry detection, so as to guarantee the accuracy of clinical related disease diagnosis.

Provided are: particles for immunonephelometry, the particles containing a metal oxide and being used for highly sensitive detection of a target substance; a reagent; a test kit; and a detection method.　The particles for immunonephelometry contain a metal oxide, and are characterized in that: the refractive index of the particles is 1.70-2.25; the volume-average particle size of the particles is 200-400 nm; and the product of the refractive index and the volume-average particle size is 340-780 nm.

Provided are a particle for immunoturbidimetry, a reagent, a test kit, and a method for detecting a target substance, each of which can detect a trace component in a low concentration region and improve storage stability. The particle for immunoturbidimetry is a particle for immunoturbidimetry including a first resin and titanium oxide, wherein the particle for immunoturbidimetry includes a first layer containing the first resin and a second layer containing the titanium oxide, wherein the second layer is arranged on the outside of the first layer, and wherein the density of the titanium oxide is 3.40 g / cm 3 Hereinafter, and wherein the content of the titanium oxide in the particle for immunoturbidimetry is 10 mass% or more and 80 mass% or less.

This invention discloses the application of sodium thiocyanate in the preparation of a kit for coagulation assays. The kit detects coagulation parameters based on coagulation methods, chromogenic substrate methods, or immunoturbidimetric assays. These coagulation parameters include prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, fibrin(ogen) degradation products, D-dimer, antithrombin III, and anti-Xa. By adding sodium thiocyanate to the coagulation assay kit, this invention optimizes the clinical relevance of the assay kit by adjusting reaction time (coagulation method), improving the linear range (chromogenic substrate method), and enhancing the differentiation between low and high values ​​(immunoturbidimetric assay), thereby improving the accuracy of the test.

The invention discloses a kit for improving detection specificity of latex immunoturbidimetry, and belongs to the technical field of immunodetection. The invention provides a composite buffer system for improving the detection specificity of latex immunoturbidimetry. The composite buffer system comprises the following components in working concentration: 5-50g / L of a sealing agent, 0.1-5mL / L of a nonionic surfactant and a specific interferent blocking agent. The invention further provides a high-specificity detection kit for the protein markers in body fluid or blood. The high-specificity detection kit comprises a reagent 1, a reagent 2, a standard substance and the composite buffer system. According to the invention, various components with different action mechanisms are creatively and scientifically matched to form a synergistic systematic solution, so that the problems of poor detection specificity and accuracy of latex immunoturbidimetry in the prior art are solved.

The invention relates to the technical field of monoclonal antibodies, in particular to an adiponectin ADP monoclonal antibody and application, the heavy chain sequence of the adiponectin ADP monoclonal antibody is shown as SEQ ID NO: 1, and the light chain sequence of the adiponectin ADP monoclonal antibody is shown as SEQ ID NO: 2. The adiponectin ADP monoclonal antibody disclosed by the invention has excellent specificity and sensitivity, can provide accurate and reliable reagent guarantee for related detection scenes, can be applied to a plurality of detection fields such as biochemical immunoturbidimetry, immunochromatography and ELISA (Enzyme-Linked Immunosorbent Assay), and realizes rapid, efficient and high-sensitivity clinical detection on an ADP antigen in serum.

The present application relates to the field of medical immunodetection, in particular to procalcitonin latex immunoturbidimetry detection reagent combination and application thereof. The present application provides procalcitonin latex immunoturbidimetry detection reagent combination comprising R1 reagent and R2 reagent and application thereof. The present application adopts latex immunoturbidimetry, and further provides procalcitonin latex immunoturbidimetry detection reagent combination with good stability, high precision, high accuracy, high anti-interference and high linear range level by optimizing reaction system and proportion of each component, which is conducive to the popularization and application of the reagent in clinic.

This invention discloses a Mycoplasma pneumoniae antibody detection kit based on immunoturbidimetry, belonging to the field of clinical immunology testing. It includes solutions R1 and R2. Solution R1 is a dispersion of double-coupled latex microspheres coated with natural Mycoplasma pneumoniae antigens, and solution R2 is a sample diluent containing an enhancer. The test samples can be serum, plasma, or whole blood samples, and it is applicable to biochemical analyzers, protein analyzers, or combined blood cell and immunoturbidimetric analyzers. The kit prepared by this invention can be used on various instruments, has a wide range of detection targets, and can be coupled with other instruments to detect various clinically valuable blood indicators, further shortening the testing time. It solves the problems of poor accuracy, low efficiency, and limited application scenarios caused by the subjective interpretation of results, long processing time, and limited applicability of blood samples in existing technologies. It can effectively simplify the testing process and improve diagnostic efficiency.

The present disclosure relates to a kit for detecting a soluble growth stimulation expressed gene 2 protein. In particular, the present disclosure relates to a latex-enhanced turbidimetric immunoassay kit for detecting the concentration and / or content of the sST2 in human samples. The kit can be used in transmission immunoturbidimetry and scattering immunoturbidimetry. The kit comprises a buffer system, an anti-interference component, latex microspheres, an anti-sST2 antibody, etc. The latex-enhanced immunoturbidimetric agent of the present disclosure can detect sST2 proteins within a range of <400 ng / ml in a sample, with a sensitivity of up to 0.1 ng / ml and a high specificity, accuracy and precision. The kit is suitable for a fully automatic biochemical analyzer and a scattering analyzer, and has the advantages of convenient and fast use and low cost, and can be used clinically to detect the sST2 protein.

This invention provides a method and reagents for measuring low concentrations of target substances using immunoturbidimetry. [Solution] A measurement method for measuring a target substance contained in a sample, wherein the target substance is a protein with a molecular weight of 150 kDa to 500 kDa, contained in the sample at a concentration of 0.8 ng / mL to 2.0 ng / mL, and comprises: a first mixing step of mixing the sample with a first reagent to obtain a first mixed solution; a second mixing step of mixing the first mixed solution with a second reagent containing particles immobilized with ligands that specifically bind to the target substance to obtain a second mixed solution; and a measurement step of measuring the absorbance of the second mixed solution, wherein the concentration of the sample in the second mixed solution is 4.0 volume% to 30.0 volume%, the mass ratio of particles to sample in the second mixed solution is 1:500 to 1:50, and the change in absorbance per 1.0 ng / mL of target substance in the measurement step is 0.0012 to less than 0.0100 for the sample.