68 results about "Immunoturbidimetry" patented technology

The invention discloses a method and equipment for detecting oxidized low-density lipoprotein based on immunoturbidimetry, and the method comprises the following steps: preparing a macroinitiator by active esterification reaction, and preparing a block graft copolymer by atom transfer radical polymerization and radical grafting reaction; the preparation method comprises the following steps: carrying out surface modification on latex microspheres with different particle sizes by using a grafted copolymer, and then respectively coupling the microspheres with different particle sizes with different OxLDL monoclonal antibodies so as to prepare a double-particle-size latex microsphere marker; and mixing the serum sample with the double-particle-size latex microsphere marker, measuring an absorbance value, and calculating the concentration of the oxidized low-density lipoprotein in the serum sample according to a standard curve established on the basis of the standard substance solution. According to the scheme, the stability of the latex microsphere marker can be enhanced, and the binding efficiency of the latex microsphere marker and ox-LDL can be improved.

The present disclosure relates to a method for increasing the sensitivity of latex immunoturbidimetry reagents. A biotin-streptavidin amplification system, a biotin-labeled antibody and streptavidin cross-linked latex microsphere are used, and the kit is characterized by comprising a first reagent and a second reagent, the first reagent comprises a buffer solution, a stabilizer, a polymerization accelerator, a preservative, a blocking agent and the biotin-labeled antibody, and the second reagent comprises a buffer solution, a stabilizer, a polymerization accelerator, a preservative, a blocking agent and a streptavidin cross-linked latex microsphere. The second reagent comprises a buffer solution, a stabilizer, latex microspheres labeled by streptavidin and a preservative. According to the present invention, the antibody is placed in the first reagent, such that the contact time of the antibody and the antigen is increased, the recognition efficiency is improved so as to improve the reaction sensitivity, and after the second reagent is added, the biotin-labeled antibody and the streptavidin latex microspheres are rapidly combined to form the agglutination so as to produce different reaction gradients, such that the sensitivity and the repeatability are significantly improved.

The invention discloses a kit for determining oxidized low-density lipoprotein based on latex enhanced immunoturbidimetry and application, and belongs to the technical field of biological detection reagents, the kit comprises a reaction buffer solution and a latex reagent, the reaction buffer solution is marked as R1, and the latex reagent is marked as R2; the reaction buffer solution comprises 3-10 g / L of a buffer solution, 5-15 g / L of inorganic salt, 10-30 g / L of a turbidity increasing agent, 0.5-2.5 mL / L of a surfactant, 1-3 mL / L of a preservative and 3-14 g / L of a stabilizer; the latex reagents are at least two latex reagents formed by coupling monoclonal antibodies and latex microspheres, and the particle size range of the latex microspheres is 200-280 nm. The latex is marked by adopting two monoclonal antibodies with relatively strong specificity, so that the specificity is relatively strong under the condition of keeping the sensitivity. According to the kit, the detection sensitivity and specificity can be improved at the same time, so that the linear range of the kit is wider, the accuracy is higher, and clinical requirements are better met.

A method, device and computer readable medium for identifying hook effect in immunoturbidimetry. The method generates a reaction curve by measuring optical signals of an immune reaction of a to-be-measured substance in a sample within a predetermined time period, and determines whether the sample has hook effect by using distribution information of the reaction curve within the predetermined time period. The method only uses measurement information of a small predetermined time period in the entire reaction time to identify samples with hook effect, and terminates the detection in time, thereby accelerating the speed of immunoturbidimetry detection of samples with hook effect.

There is a need to quantify both low and high concentrations of target substances with high sensitivity. [Solution] The present invention provides an immunoturbidimetric reagent comprising a first particle and a second particle, wherein the ratio of the volume-average particle size (Dv2) of the second particle to the volume-average particle size (Dv1) of the first particle, Dv2 / Dv1, is 0.37 or more and 0.86 or less, the volume-average particle size of the second particle is 150 nm or more, and the ratio of the refractive index (R2) of the second particle to the refractive index (R1) of the first particle, R2 / R1, is 0.87 or more and 0.97 or less.

The present disclosure relates to a kit for detecting a soluble growth stimulation expressed gene 2 protein. In particular, the present disclosure relates to a latex-enhanced turbidimetric immunoassay kit for detecting the concentration and / or content of the sST2 in human samples. The kit can be used in transmission immunoturbidimetry and scattering immunoturbidimetry. The kit comprises a buffer system, an anti-interference component, latex microspheres, an anti-sST2 antibody, etc. The latex-enhanced immunoturbidimetric agent of the present disclosure can detect sST2 proteins within a range of <400 ng / ml in a sample, with a sensitivity of up to 0.1 ng / ml and a high specificity, accuracy and precision. The kit is suitable for a fully automatic biochemical analyzer and a scattering analyzer, and has the advantages of convenient and fast use and low cost, and can be used clinically to detect the sST2 protein.

The invention discloses a method for weakening the influence of lipemia on transmission-scattering fusion enhanced immunoturbidimetry detection. The method specifically comprises the following steps: S1, measuring the mean value of absorbance signal values of photometric points in a specific section after a reaction buffer solution is added into each sample; s2, measuring non-lipid turbidity values of different samples which do not cause low scattering measurement after each sample is added into the reaction buffer solution, namely, a background; s3, comparing the value measured in the step S1 with the value measured in the step S2 so as to judge whether the scattering turbidimetry measurement result needs to be revised or not; the invention relates to the technical field of transmission-scattering fusion enhanced immunoturbidimetry detection. According to the method for weakening the influence of the lipid blood on the transmission-scattering fusion enhanced immunoturbidimetric assay, when the transmission-scattering fusion enhanced immunoturbidimetric assay is carried out, the turbidity of fat particulate matters in a lipid blood sample is strongly correlated with a transmission turbidity signal value of the sample added into a reaction buffer solution, so that the influence of the lipid blood on the transmission-scattering fusion enhanced immunoturbidimetric assay is reduced; meanwhile, the relevance is specifically numeralized through the transmission light absorbance value of the sample and the R1.

The invention belongs to the technical field of biological detection, and particularly relates to a detection method and a detection kit for oxidized low-density lipoprotein. The invention provides a novel application of quaternary ammonium salt chitosan in a kit, and particularly relates to application of quaternary ammonium salt chitosan with different molecular weights in an immunoturbidimetry detection process of oxidized low-density lipoprotein. When high-molecular-weight quaternized chitosan with the molecular weight of 100-200 kDa and low-molecular-weight quaternized chitosan with the molecular weight of 30-50 kDa are used as components of the oxidized low-density lipoprotein detection kit, the quaternized chitosan with different molecular weights can react with citrate radicals to form gel particles with different mechanical strengths; the content of the oxidized low-density lipoprotein can be detected through immunoturbidimetry, and the sensitivity and the linear range of the oxidized low-density lipoprotein detection kit are greatly improved and widened by using the quaternized chitosan with different molecular weights.

The application provides a lipoprotein phospholipase A2 determination kit for latex immunoturbidimetry, which contains reagent R1 and reagent R2; the reagent R1 comprises the following components: buffer, inorganic salt, anti-interference additive, surfactant and Proclin 300; the reagent R2 comprises the following components: buffer, lipoprotein phospholipase A2 antibody coated latex microspheres, stabilizer, surfactant and Proclin 300. The kit is a liquid kit with strong stability, high accuracy, wide linear range and strong anti-HOOK capability.

The invention discloses a kit for latex immunoturbidimetric determination of urine immunoglobulin G, and relates to the field of biological detection technology. The kit includes R1 reagent and R2 reagent; the R1 reagent includes 10-200mM tris(hydroxymethyl)aminomethane, 1-10g / L bovine serum albumin, 10-50g / L reaction enhancer, 0.1-10mL / L preservative, 100-300mM NaCl, 2-6g / L surfactant; the R2 reagent includes 10-200mM tris(hydroxymethyl)aminomethane, 100-300mM NaCl, 1-5g / L antibody-coupled latex particles, 1-5g / L glycerol, 5-10g / L methyl-β-cyclodextrin, 1-10g / L bovine serum albumin, 2-6g / L surfactant and 0.1-10mL / L preservative. The kit of the present invention has good repeatability and stability.

The invention relates to the technical field of antibody immunological detection, in particular to a polypeptide based on PDZK1IP1 protein, an antibody and application of the polypeptide and the antibody. On the first aspect, the invention discloses a polypeptide for preparing a tumor marker PDZK1IP1 antibody, and the polypeptide is selected from the 68-81th amino acid sequence of PDZK1IP1 protein. The second aspect discloses a polyclonal antibody based on the polypeptide, the polyclonal antibody is used for preparing or screening a pulmonary nodule detection reagent, and the expression quantity of the PDZK1IP1 protein in a subject is reflected through the polyclonal antibody. On the third aspect, the invention discloses a kit for detecting lung cancer related PDZK1IP1 protein, which comprises latex particles coated with a polyclonal antibody on the surface, and a standard substance obtained by expressing and purifying a recombinant expression vector of a PDZK1IP1 protein gene complete sequence. The invention discloses a non-invasive biomarker protein for distinguishing benign and malignant pulmonary nodules for the first time, and develops a detection method based on immunoturbidimetry and a corresponding diagnostic kit accordingly, thereby providing a novel non-invasive, rapid, sensitive and high-specificity detection approach for early lung cancer.

The present invention relates to the field of biological detection technology, and in particular to a detection kit and method for eliminating nonspecific reactions in blood samples. The present invention provides a detection kit comprising a first reagent and a second reagent; the first reagent comprises a buffer solution, 0.01-0.5wt% sodium alginate, 0.05-1wt% nonionic surfactant, and 0.001-0.1wt% animal serum; the pH value of the first reagent buffer system is 7.2-7.4; the second reagent comprises an antibody buffer solution labeled with latex microspheres; the pH value of the second reagent buffer system is 7.8-8.0. The present invention provides a detection kit and its application, which can effectively eliminate the interference of nonspecific reactions on the detection results of biochemical indicators by using latex immunoturbidimetry to detect biochemical indicators in serum and plasma, thereby improving the accuracy of the detection results of biochemical indicators.

The invention provides a cTnI protein antibody. The cTnI protein antibody comprises an anti-human cTnI protein monoclonal antibody I, an anti-human cTnI protein monoclonal antibody II and / or an anti-human cTnI protein monoclonal antibody III, the antibody respectively comprises a light chain and a heavy chain; wherein the light chain sequence of the antibody I is as shown in SEQ ID NO.1, and the heavy chain sequence of the antibody I is as shown in SEQ ID NO.2; a light chain sequence of the antibody II is as shown in SEQ ID NO.3, and a heavy chain sequence of the antibody II is as shown in SEQ ID NO.4; the light chain sequence of the antibody III is as shown in SEQ ID NO.5, and the heavy chain sequence of the antibody III is as shown in SEQ ID NO.6. The invention also provides a latex enhanced immunoturbidimetric kit for quantitatively detecting the cTnI protein and a preparation method of the latex enhanced immunoturbidimetric kit. The invention has the advantages that the antibody affinity is high, the specificity is good, and a kit based on the antibody is high in sensitivity, good in specificity and strong in anti-interference capability.

The invention provides a heparin binding protein detection kit, the kit comprises a reagent I and a reagent II, the reagent I is a buffer solution containing a surfactant and a macromolecular polymer; and the reagent II is a buffer solution of polystyrene latex microspheres containing monoclonal antibody pairs coated with anti-human heparin binding protein. The invention solves the technical problem that in the prior art, the latex enhanced immunoturbidimetry is adopted to improve the linear upper limit, so that the low-value repeatability is poor, and the positive and negative judgment in clinical diagnosis is influenced.

The invention discloses a polymeric antibody for immunoturbidimetry as well as a preparation method and application thereof, relates to the technical field of in-vitro detection, and comprises a polymeric technology based on a polymer skeleton coupling antibody, and the polymeric antibody prepared by the technology has a multivalent effect, can replace the existing monoclonal antibody or secondary antibody immunoturbidimetry reagent, and can be applied to immunoturbidimetry. The HOOK-resistant monoclonal antibody can be well applied to various immunoturbidimetric biochemical reagents, not only has extremely high sensitivity to low-value samples, but also has an excellent HOOK-resistant effect on high-value samples, and meanwhile, avoids different stability problems caused by dynamic combination of a secondary antibody detection system.

The invention discloses a kit for detecting C-reactive protein in whole blood by latex enhanced immunoturbidimetry and a manufacturing process of the kit, and relates to the technical field of clinical in-vitro diagnosis. According to the kit, aiming at the requirement of directly detecting the C-reactive protein in a whole blood sample, a latex enhanced immunoturbidimetry is adopted, and the detection sensitivity of a turbidity signal is enhanced through specific binding of latex microspheres coated with an anti-CRP antibody and the C-reactive protein in the sample. The kit comprises the optimized antibody coated latex microspheres and other matched reagents, a whole blood sample can be directly used, and a serum or plasma separation step is not needed, so that the operation is simplified, and the detection time is shortened. According to the preparation process, the stability of reagent components and the batch-to-batch consistency are ensured through a specific technology. The kit provided by the invention has the advantages of rapidness, accuracy, simplicity and convenience in operation and the like, can be compatible with conventional laboratory equipment, and provides an efficient and reliable detection means for evaluation of clinical inflammation and infectious diseases.

The invention relates to a triglyceride separation device based on a membrane filtration technology, in particular to a device applied to separation of triglyceride, protein and the like in serum. The device effectively removes triglyceride particles in serum without influencing the detection of other biochemical components (such as various proteins and the like) by designing a membrane with a proper aperture and combining a centrifugal separation technology. The membrane is made of polyether sulfone (PES) or polytetrafluoroethylene (PTFE) and other materials with good chemical stability and biocompatibility, the pore size of the membrane is designed between 30 nanometers and 80 nanometers, and triglyceride particles can be effectively separated. The device is simple, convenient, low in cost and suitable for rapid treatment of lipid turbidity samples in medical examination, the problem of lipid turbidity interference in the prior art is solved, and the accuracy of biochemical detection such as immunoturbidimetry is improved.

The invention discloses a latex enhanced immunoturbidimetry detection reagent for glycosylated hemoglobin and a use method of the latex enhanced immunoturbidimetry detection reagent, and relates to the technical field of medical examination. The latex enhanced immunoturbidimetry detection reagent for glycosylated hemoglobin comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises first nano microspheres; and the reagent R2 comprises a second nano microsphere coated with a monoclonal antibody of HbA1c. According to the reagent R2, the nano microspheres are coated with the monoclonal antibody of HbA1c, the stability risk caused by the fact that balance needs to be established for immunoreaction of multiple antibodies and antigens is avoided through the monoclonal antibody, the problem that a glycosylated hemoglobin immunoturbidimetry detection reagent is insufficient in stability can be solved, the nano microspheres are arranged on the reagent R2, and therefore the stability of the glycosylated hemoglobin immunoturbidimetry detection reagent is improved, and the stability of the glycosylated hemoglobin immunoturbidimetry detection reagent is improved. By utilizing the characteristic that the volume of an antigen-antibody conjugate can be increased after the nano-microspheres are combined with the HbA1c monoclonal antibody, the turbidimetric degree of latex is effectively amplified, and the detection sensitivity can be improved.

The present invention discloses a reagent set, a kit and a method for performing immunoassay using the same. Also disclosed is a method for preparing a latex enhanced immunoturbidimetry reagent, which comprises the following steps: after activating large-diameter and small-diameter latex microspheres respectively, adding an antibody against the object to be detected to the large-diameter latex microspheres, and adding the protein complex of the object to be detected to the small-diameter latex microspheres to perform a coupling reaction; after the reaction solution is respectively sealed, centrifuged, redissolved and resuspended, an R1 reagent containing antibody-coupled large-diameter latex microspheres and an R2 reagent containing small-diameter latex microspheres coupled to the object to be detected are obtained. The kit of the present invention comprises: the R1 reagent, the R2 reagent and a calibrator. The present invention gives full play to the advantages of latex microspheres of different particle sizes, and extremely overcomes the problems of low sensitivity and detection linear range that cannot be taken into account in the existing methods, and has the advantages of accurate measurement, simple and fast operation, can be used for outpatient and emergency detection, and the instrument has a wide range of applicability.

A creatine kinase isoenzyme latex-enhanced immunoturbidimetric assay kit, comprising a first reagent and a second reagent. The first reagent comprises a buffer solution, an electrolyte, polyethylene glycol, a surfactant, a preservative, a blocking agent, and a protective agent. The second reagent comprises a buffer solution, polystyrene latex particles coated with a creatine kinase isoenzyme antibody, the creatine kinase isoenzyme antibody on the latex particles, a protective agent, a stabilizer, and a preservative.

The invention discloses a latex enhanced immunoturbidimetric kit for detecting free hemoglobin, and belongs to the technical field of medicine detection. The latex enhanced immunoturbidimetric kit for detecting the free hemoglobin comprises a reagent R1 and a reagent R2, the reagent R1 comprises 50 mM of tris (hydroxymethyl) aminomethane, 0.5 g / L to 2 g / L of a coagulant, 50 mg / L to 200 mg / L of a blocking agent, 0.5 g / L to 1.5 g / L of sodium chloride, 0.05 g / L to 0.15 g / L of bovine serum albumin, 0.25 g / L to 0.75 g / L of trehalose and 0.05 g / L to 0.5 g / L of a preservative; the reagent R2 is prepared from 50 mM of hydroxyethyl piperazine ethanesulfonic acid, 0.5 g / L to 1.5 g / L of sodium chloride, 0.05 g / L to 0.15 g / L of bovine serum albumin, 3 g / L to 7 g / L of glycerinum, 1 g / L to 3 g / L of trehalose, 0.05 g / L to 0.5 g / L of preservative and 0.05 g / L to 0.2 g / L of anti-human hemoglobin antibody sensitized latex particles. Through chemical coupling of a hemoglobin monoclonal antibody and carboxyl microspheres, an'antigen-monoclonal antibody-microsphere-monoclonal antibody-antigen 'compound detection system is constructed.

The present invention relates to the field of biological detection technology and provides an immunoglobulin E antibody comprising a heavy chain and a light chain; the amino acid sequence of the heavy chain is shown in SEQ ID NO. 1, and the amino acid sequence of the light chain is shown in SEQ ID NO. 2. The present invention also provides a kit for determining IgE using a latex-enhanced immunoturbidimetric assay comprising the immunoglobulin E antibody. The present invention has the advantages of simple operation, high linearity, good stability, and high sensitivity, accuracy, and specificity when using the immunoglobulin E antibody of the present invention in combination with a corresponding latex-enhanced immunoturbidimetric assay kit for determining IgE.

The invention relates to an S-4FB glucosamine modified carboxyl microsphere for latex immunoturbidimetry, a synthesis method and application. On the basis of carboxyl polystyrene microspheres, the S-4FB-glucosamine modified microspheres for latex immunoturbidimetric determination are obtained through glucosamine modification and S-4FB modification, the prepared S-4FB-glucosamine modified carboxyl microspheres have active groups and can be directly coupled with biomolecules with amino groups, operation is easy, convenient and efficient, coupling efficiency is high, and the S-4FB-glucosamine modified carboxyl microspheres have good application prospects. The anti-interference capability is strong, and the stability is good. When the S-4FB-glucosamine modified carboxyl microspheres are used as indicating microspheres for preparing a detection reagent, the repeatability, linearity, acceleration stability and kit correlation of the reagent are superior to those of unmodified carboxyl polystyrene microspheres. Therefore, the S-4FB-glucosamine modified carboxyl microspheres disclosed by the invention have a wide application prospect in the aspect of in-vitro detection.

The application provides a latex immunoturbidimetric detection reagent, a kit and a detection method. The latex immunoturbidimetric detection reagent comprises a buffer and an ionic liquid, the ionic liquid comprises an ionic liquid containing an imidazole structure, the ionic liquid containing the imidazole structure comprises 1-alkyl-3-methyl imidazole halide or 1-alkyl-3-methyl imidazole fluoride, the halogen in the 1-alkyl-3-methyl imidazole halide comprises chlorine, bromine or iodine, and the alkyl in the 1-alkyl-3-methyl imidazole halide or 1-alkyl-3-methyl imidazole fluoride comprises alkyl containing 2, 3, 4, 5, 6 or 7 C atoms. The application can solve the problem of a small linear range in the prior art latex turbidimetric method, and is suitable for the field of immunoturbidimetric detection.

The invention discloses a serum COVID-19 NAb quantitative detection reagent as well as preparation and application thereof, and belongs to the technical field of biomedical detection. The latex microsphere reagent contained in the quantitative detection reagent is produced by adopting a continuous process, so that complicated purification processes such as centrifugation and washing are avoided, the preparation time is remarkably shortened, and the complexity of the production process and the difference between batches are reduced. Meanwhile, by optimizing a buffer solution system and reaction conditions, the detection sensitivity and the linear range are improved, the linear range is 122.46 ng / mL to 10000 ng / mL, the correlation coefficient R2 is equal to 0.9999, the absolute relative deviation does not exceed + / -1.88%, the detection requirements of neutralizing antibodies (NAb) with different concentrations in clinical samples can be met, and the defect that the linear range of a traditional method is narrow is overcome. The reagent is based on an immunoturbidimetry principle, the detection process can be carried out on a full-automatic biochemical analyzer, the operation is simple and convenient, and the detection is efficient.

The application discloses an anti-dextran single-chain antibody and application. The amino acid sequence of the anti-dextran single-chain antibody is shown as SEQ ID NO. 6, and further, a COMP sequence is added at the C terminal of the single-chain antibody to form a derivative variant A48-COMP, and the amino acid sequence of the derivative variant of the single-chain antibody is shown as SEQ ID NO. 8. The derivative variant A48-COMP can produce turbidity with dextran, and can be directly applied to an immune turbidimetry method for measuring dextran. Compared with a general immune turbidimetry method, the application uses a genetic engineering antibody, is easy to prepare, has low detection cost, and has the advantages of simplicity and rapidness.

The invention discloses a reagent, a kit and a detection method for free light chain detection, and relates to the technical field of detection kits, the reagent comprises the following components: a solid phase carrier surface marked with an F (ab ') 2 antibody fragment; the liquid-phase reaction liquid comprises a composite blocking agent for inhibiting non-specific reaction of endogenous interferents; wherein the F (ab ') 2 antibody fragment and the composite blocking agent act together to improve the detection specificity and reaction linearity. The problems that non-specific reaction is caused by an antibody Fc segment and an endogenous interferent when a latex enhanced immunoturbidimetric assay is used for detecting free light chains, existing means cannot eradicate the non-specific reaction, detection linearity is abnormal, and specificity is low are solved, the solid phase carrier marked with the F (ab ') 2 antibody segment and the composite blocking agent are used, and the solid phase carrier marked with the F (ab') 2 antibody segment and the composite blocking agent have a synergistic effect; closed-loop protection is formed from two dimensions of eliminating self interference of the antibody and shielding exogenous interference of the sample, and compared with an antibody optimization or interference shielding means, the combination targeting and detection specificity of a target analyte are synergistically improved.

The present application relates to the technical field of antibody immunology detection, and particularly relates to a polypeptide based on PDZK1IP1 protein, an antibody and application thereof. A polypeptide for preparing a tumor marker PDZK1IP1 antibody is disclosed in the first aspect, and the amino acid sequence at positions 68-81 of the PDZK1IP1 protein is selected. A polyclonal antibody based on the polypeptide is disclosed in the second aspect, and is used for preparing or screening a lung nodule detection reagent, and the expression amount of the PDZK1IP1 protein in a subject is reflected by the polyclonal antibody. A kit for detecting lung cancer related PDZK1IP1 protein is disclosed in the third aspect, and the kit comprises latex particles coated with the polyclonal antibody, and a standard product obtained by expression and purification of a recombinant expression vector of the whole sequence of the PDZK1IP1 protein gene. The present application discloses a non-invasive biomarker protein for distinguishing benign and malignant lung nodules for the first time, and develops a detection method and a corresponding diagnostic kit based on the immunoturbidimetry technology, so as to provide a new detection approach of non-invasion, rapidness, sensitivity and high specificity for early lung cancer.

This invention belongs to the field of bioassay technology, and specifically relates to a method and kit for detecting oxidized low-density lipoprotein (ODL). This invention provides a novel application of quaternized chitosan in the kit, specifically using quaternized chitosan of different molecular weights in the immunoturbidimetric detection of ODL. Results show that when high molecular weight quaternized chitosan (100-200 kDa) and low molecular weight quaternized chitosan (30-50 kDa) are used as components of the ODL detection kit, the different molecular weights of quaternized chitosan react with citrate to form gel particles of varying mechanical strengths. This allows for the detection of ODL content via immunoturbidimetry. Furthermore, the use of quaternized chitosan of different molecular weights significantly improves the sensitivity and linear range of the ODL detection kit.

The invention belongs to the field of preparation of affinity polymer microspheres, and relates to PS latex particles with high reactivity with a pepsinogen antibody as well as a preparation method and application of the PS latex particles. Crosslinked polystyrene is adopted as an inner core, the structural stability and the refractive index property of the latex particle are guaranteed, the acrylate monomer and the acrylic copolymer are adopted as a shell layer, adjustable carboxyl reaction sites are provided on the surface of the latex particle, and the polyacrylate component with the low glass transition temperature has the rubbery state characteristic, so that the stability of the latex particle is improved, and the service life of the latex particle is prolonged. The carboxyl on the shell layer has higher reaction efficiency. The carboxylated PS latex nanoparticles can be used as a raw material of a kit for latex turbidimetry detection of gastric cancer markers, the reaction efficiency of latex particles and pepsinogens (PGI and PGII) is improved, the detection performance of immunoturbidimetry is enhanced, and the obtained latex kit has increased detection sensitivity and a linear range.