311 results about "Immunoturbidimetry" patented technology

The invention relates to a kit for determining heart-type fatty acid binding protein in serum or urine by latex enhanced turbidimetric immunoassay. Specifically, the kit for determining the heart-type fatty acid binding protein comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 contains a reaction promoter, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; the reagent R2 contains latex particles with binding of anti-heart-type fatty acid binding protein monoclonal antibody and polyclonal antibody, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; and the calibrator contains an antiseptic, an electrolyte, a stabilizing agent, a heart-type fatty acid binding protein pure product and a buffer. By the complex coating method of latex particles with the monoclonal antibody and the polyclonal antibody, high sensitivity and wide linear range of the kit are guaranteed. Simultaneously, the kit also has advantages of high accuracy, good repeatability, strong singularity, easy operation and the like, and is applicable to an automatic biochemical analyzer which is commonly used in clinic.

The invention provides a full-scale CRP colloidal gold immunoturbidimetric assay kit. A quantitative assay purpose is reached through enhancing the turbidity by the colloidal gold. The method used by the kit provided by the invention solves a problem that the generation of a precipitate after a latex reinforced immunoturbidimetric reaction goes against biochemical instrument cleaning. The kit provided in the invention comprises a reagent R1 and a reagent R2, wherein the reagent R2 is a proper buffer solution; and the reagent R2 is a buffer solution of the colloidal gold combined with an antihuman CRP antibody. The kit has the characteristics of high sensitivity, strong specificity and good stability, can be used for assaying the content of the CRP in serum or blood plasma, and is suitable for clinical fully-automatic biochemical analyzers. The CRP assay sensitivity of the kit can reach 0.01mg / L, and the upper CRP assay limitation of the kit is 500mg / L.

The invention relates to a kit for determination of human alpha-fetoprotein content by a latex-enhanced immunoturbidimetry. The kit provided by the invention comprises an alpha-fetoprotein R1 reagent, an alpha-fetoprotein R2 reagent and an alpha-fetoprotein calibrator, wherein the alpha-fetoprotein R1 reagent comprises one or more electrolytes, a coagulation accelerator, one or more stabilizing agents, one or more surfactants, an antiseptic and a buffer solution; the alpha-fetoprotein R2 reagent comprises latex particles coated by polyclonal antibodies capable of resisting a human alpha-fetoprotein, one or more electrolytes, one or more stabilizing agents, one or more surfactants, an antiseptic and a buffer solution; and the alpha-fetoprotein calibrator comprises an antiseptic, one or more electrolytes, one or more stabilizing agents, alpha-fetoprotein antigens and a buffer solution. The kit has strong specificity and high sensitivity, is homogeneous and stable, can be used fast and conveniently and can be used for various biochemical analyzers.

The invention relates to a kit for detecting brain natriuretic peptide in blood serum and provides the kit for detecting brain natriuretic peptide by nano microsphere immunonephelometry, which has the characteristics of no need of dilution of a sample, simple operation, high precision, good repeatability and suitability for a clinically common used automatic biochemical analyzer, in order to solve technical problems. The invention adopts a technical scheme that the kit for detecting the brain natriuretic peptide by nano microsphere immunonephelometry comprises the following components: a, a reagent R1 which comprises buffer solution, a preservative, a stabilizing agent, an electrolyte, a surfactant, and the balance of water; b, a reagent R2 which comprises buffer solution, nano microspheres combined with brain natriuretic peptide antibodies and a preservative, wherein the diameter of the microspheres is 50 to 150 nm; and c, a reference calibration material which comprises buffer solution, a stabilizing agent, a preservative, a recombinant brain natriuretic peptide pure product which is added in a corresponding amount according to the required concentration of the BNP (brain natriuretic peptide) reference calibration material, and the balance of water.

The invention relates to a kit for measuring beta2-microglobulin in serum. The technical problem to be solved is to overcome the disadvantages of background technology and provide a kit for detecting the beta2-microglobulin by nanometer microsphere immunoturbidimetry, which has no need of dilution of a sample, simple operation, high accuracy and good repeatability, and is applicable to full automatic biochemical analyzers with diverse types. The technical scheme comprises that: the kit for detecting the beta2-microglobulin by the nanometer microsphere immunoturbidimetry comprises a reagent R1, a reagent R2 and a reference calibration material, wherein the reagent R1 comprises buffer solution, a preservative, a stabilizing agent, an electrolyte, a surfactant and the balance of purified water; the reagent R2 comprises the buffer solution, nanometer microspheres which is combined with an anti-human beta2-microglobulin antibody and has a diameter of between 50 and 150 nm and the preservative; and the reference calibration material comprises the buffer solution, the stabilizing agent, the preservative, a recombinant human beta2-microglobulin pure product in a proper amount determined by concentration requirement and the balance of the purified water.

The invention relates to a latex enhanced immunoturbidimetry kit for the detection of asymmetric dimethylarginine content. Specifically, the invention relates to a latex enhanced immunoturbidimetry kit for the detection of asymmetric dimethylarginine (ADMA) content in human blood samples. The kit comprises a mouse anti-human monoclonal antibody solution, an ADMA-human Serum albumin complex crosslinked latex particle expansion and an ADMA calibrator. According to the method, by the utilization of competitive binding of free ADMA in blood and ADMA crosslinked on the surface of latex particles to ADMA monoclonal antibody, turbidity formed by the reaction between the ADMA-crosslinked latex particles and ADMA monoclonal antibody is reduced. Therefore, the content of ADMA is detected through the reduction degree of turbidity. The kit provided by the invention can be applied in a biochemical analyzer commonly-used in clinic, is convenient and rapid to operate and has strong specificity. Both sensitivity and detection range of the kit can satisfy clinic application needs.

The invention discloses a procalcitonin (PCT) latex enhanced immunoturbidimetry detection kit and a preparation method thereof. The procalcitonin latex enhanced immunoturbidimetry detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 mainly comprises a buffer solution 1, a stabilizing agent 1, a preservative 1, a coagulation increasing agent, a protective agent 1 and EDTA (Ethylene Diamine Tetraacetic Acid); the reagent R2 mainly comprises a buffer solution 2, a stabilizing agent 2, a preservative 2, a polystyrene latex microballon sphere, a PCT antibody and a protective agent 2; the particle size of the polystyrene latex microballon sphere in the reagent R2 is 100-600nm, the PCT antibody is one or more of a mouse anti-human PCT antibody, a goat anti-human procalcitonin antibody or a rabbit anti-human procalcitonin antibody, and the PCT antibody is connected with the polystyrene latex microballon sphere in a covalent coupling or physical absorption manner. Compared with the prior art, the PCT detection kit provided by the invention has the advantages of low preparation cost, good stability, high detection sensitivity and strong specificity, is easy to store, and is easily popularized in clinical.

The invention relates to a double antibody latex enhanced retinol binding protein detection kit. More specifically, the invention discloses a double antibody coating latex enhanced immunoturbidimetry kit for detecting retinol binding protein. The kit contains a reagent 1, a reagent 2 and a calibrator. Paring monoclonal antibody A and B are respectively coated on latex particles. Coated antibody is bonded with RBP to be detected, and a plurality of bonders are aggregated together to form detectable turbidity change. The detection kit provided by the invention has high sensitivity, can be used to detect urine samples and serum samples and can be used as nutritive index to detect RBP in serum. Simultaneously, through the detection of RBP in urine, the kit is sensitive to the damage degree of renal proximal tubule.

The invention relates to a kit of latex-enhanced immunoturbidimetry for detecting content of glycocholic acid in blood serum. Specifically, the provided glycocholic acid detection kit comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 comprises a reaction promotion agent, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; the reagent R2 comprises latex particles combined with a glycocholic acid antibody, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; and the calibrator comprises a preservative, an electrolyte, a stabilizer, glycocholic acid pure products and a buffer solution. The kit for detecting the content of the glycocholic acid in the blood serum, disclosed by the invention, ensures the high sensitivity and wide linear range of a kit by utilizing a method of coating the latex particles by utilizing polyclonal antibodies, also has the advantages of high accuracy, good repeatability, strong specificity, simplicity in operation and the like, and can be applied to a clinical general full automatic biochemical analyzer.

The invention provides a gastric cancer detecting method, a reagent and a gastric cancer detecting kit. The kit comprises a reagent R1 and a reagent R2 or R3; in vitro, the serum of a tested body is mixed with the reagent R1, and the reagent R2 or R3 is added after several minutes; latex particles included in the reagent R2 or R3 are coated with PG antibodies and can be combined with the PG antigen in a sample to form an insoluble complex; based on a latex-enhanced immunoturbidimetry principle, the absorbancy tested by a biochemical analyzer is compared with that of a calibration product, so that the content level of PG is calculated; compared with the content level of the serum PG of healthy people, the content level of PG shows that a health condition of the stomach of the tested human body is initially judged. By comparing a test result obtained by using the kit with that obtained by using an imported kit A, the kit has the advantages that the extremely-high correlation is achieved, and the correlation coefficient is more than or equal to 0.9750. The kit provided by the invention can be used for detecting the gastric cancer, and has stable and reliable detection results; moreover, the use cost can be reduced.

The invention relates to the field of biotechnology and discloses a kit for detecting fibrin (ogen) degradation products (FDP) by using emulsion immunoturbidimetry. The kit comprises an anti-human FDP antibody emulsion which is formed by performing chemical crosslinking of acidulated anti-human FDP antibodies and polyvinyl benzyl chloride emulsion particles and sealing the reaction product. The kit of the invention has the advantages that: the emulsion immunoturbidimetry is adopted to detect the FDP content, so the sensitivity is high and can reach 0.10 mu g / ml; the stability is high, the operation is simple and quick and it only takes 5 to 10 minutes to obtain a detection result; the specificity is high and the detection avoids interference effectively; and the quantification is accurateand the application range is wide.

The invention relates to the technical field of biotechnology, and particularly discloses a kit for detecting retinol binding protein content by using latex immunoturbidimetry. The kit comprises a reagent R1, a reagent R2 and a standard product, wherein the reagent R1 is buffer solution with pH (Potential of Hydrogen) value of 6-9; the reagent R2 is latex reagent coated by anti-retinol binding protein double antibodies; and the standard product is retinol binding protein solution with pH value of 5-8. According to the kit, the retinol binding protein content in a sample can be detected by using the latex immunoturbidimetry; the sensitivity is high and can reach 0.042 mg / L; and the kit has the advantages of high stability, easiness and quickness in operation, high specificity, low probability of interference, accurate quantification and broad application prospect.

The invention provides a kit for measuring adiponectin by a nano latex enhanced immune turbidimetry. The kit comprises reagent R1, reagent R2 and adiponectin antigen calibration material solution, wherein the reagent R1 comprises electrolyte, a stabilizer, a surface active agent, a preservative and buffer solution; the reagent R2 comprises latex particles coated by anti-human adiponectin polyclonal antibody, electrolyte, a stabilizer, a surface active agent, a preservative and buffer solution; the latex particles are copolymer formed by styrene and acrylic acid; the adiponectin antigen calibration material solution comprises adiponectin and a stabilizer; the surfaces of the latex particles in the reagent R2 have carboxyl groups, and the particle size range of the latex particles is 80-100nm. The kit is simple to operate, rapid and accurate in measurement, high in sensitivity and specificity, and low in detection cost.

A blood coagulation analyzer and analyzing method perform following: (a) preparing a measurement specimen by dispensing a blood specimen and a reagent into a reaction container; (b) emitting light of a plurality of wavelengths to the measurement specimen in the reaction container, the wavelengths comprising a first wavelength for use in a measurement by a blood coagulation time method, and at least one of a second wavelength for use in a measurement by a synthetic substrate method and a third wavelength for use in a measurement by an immunoturbidimetric method; (c) detecting light of a plurality of wavelengths corresponding to the light emitted in (b), from the measurement specimen, by a light receiving element, and acquiring data corresponding to each wavelength; and (d) conducting an analysis based on the data corresponding to one of the wavelengths among the acquired data, and acquiring a result of the analysis.

The invention discloses an anti-cyclic citrullinated peptide (CCP) antibody detection kit. The kit comprises a reagent R1, a reagent R2, a standard substance and a standard diluent, wherein the reagent R1 is prepared by washing CCP antigen latex particles by 50mM Tris buffer solution containing 20-500mmol / L first stabilizer and 0.1-1% preservative and then dispersing; and CCP antigens and polystyrene latex microspheres are subjected to chemical crosslinking and then are closed, so that the CCP antigen latex particles are formed. For the kit, the latex-enhanced immunoturbidimetry is adopted for detecting the content of an anti-CCP antibody, the kit has the high sensitivity, the high stability and the high detection speed (the time from determination to result acquisition is only within 5-10min), can detect mass samples on a conventional biochemical analyzer and can greatly improve the detection efficiency.

The invention relates to the technical field of medical examination, and particularly relates to a latex immunoturbidimetry serum pepsinogen I detection kit for eliminating chyle interference. The kit provided by the invention comprises (1) a pepsinogen I calibrator; (2) a reagent 1 with a preset chyle remover; and (3) a reagent 2 containing the monoclonal antibody and polyclonal antibody coated latex particles against human pepsinogen I. According to the kit provided by the invention, the combination reaction between a substrate to be detected in a sample and a specific antibody in the reagent is amplified through the latex agglutination effect; with the given wavelength, the turbidity formed by the reaction is related to the content of the substrate to be detected, thus the content of the substrate to be detected is calculated. The kit provided by the invention is used for detecting the content of pepsinogen I in human serum, has high sensitivity and good specificity, and can eliminate chyle interference in the sample; moreover, the kit is simple and quick to operate and has high practicability and wide application range.

The invention discloses a preparation method and application of D-dimer immuno latex microspheres. In the preparation method of D-dimer immuno latex microspheres, a chemical crosslinking method is optimized, the carboxyl of polystyrene microsphere is activated in a low-pH condition and then coupled with amino of an antibody dissolved in a high-pH condition, and the mixture of the two is neutral and is coupled for 12-24h at a low temperature, so that not only can the coupling efficiency of the antibody be guaranteed, but also the damage of the antibody activity caused by an activator is reduced to a great extent. A latex enhanced turbidimetric immunoassay D-dimer test kit prepared from the immuno latex microspheres prepared by the method has the characteristics of high sensitivity, accurate quantification, good repeatability and stable property, and the lowest detection limit can reach 0.01mg / L; the latex enhanced turbidimetric immunoassay D-dimer test kit can be applied to a full-automatic biochemical analyzer or coagulation analyzer, is quick and easy to operate, only needs 5-10 minutes from detection to result collection and has relatively good clinical application prospect.

The invention relates to the technical field of medical examination and particularly relates to a latex immunoturbidimetry type pepsinogen II detection kit capable of eliminating chyle interference. The kit provided by the invention comprises: (1) a pepsinogen II calibrator; (2) a reagent 1 with a preset chyle remover; and (3) a reagent 2 containing a monoclonal antibody of anti-human pepsinogen II and polyclonal antibody coated latex particles. According to the kit using the method, a conjugation reaction of substance to be detected in a sample and specific antibodies in the reagents is amplified by a latex agglutination effect, turbidity formed by the reaction is associated with the content of the substance to be detected under a given wavelength, so that the content of the substance to be detected can be calculated. The kit provided by the invention is used for detecting the content of pepsinogen II in human serum, and the kit is high in sensitivity, good in specificity, capable of eliminating chyle interference in the sample, quick and convenient to operate, strong in practicability and is wide in application range.

The invention relates to a latex enhanced immunoturbidimetry kit for measuring the content of C peptide in human serum, a preparation method of the kit and a method for detecting the content of C peptide in human serum by use of the kit. The kit comprises a first reagent, a second reagent and a calibrator, wherein the first reagent contains buffer solution, electrolyte, a surface active agent, a stabilizing agent, a reaction enhancer and a preservative; the second reagent contains buffer solution, electrolyte, a surface active agent, a preservative and anti-human C peptide antibody enveloped sensitization polystyrene latex particles; and the calibrator contains buffer solution, a surface active agent, a stabilizing agent, a preservative and human C peptide protein. Compared with the existing kit used clinically, the kit provided by the invention has strong specificity, high sensitivity and high accuracy, the kit is suitable for biochemical analyzers of common detection mechanism, has short detection time, can be operated conveniently, has low detection cost, has no radioactive contamination, and is suitable for popularization and application clinically.

The invention discloses a latex-enhanced immunoturbidimetry kit for measuring a Helicobacter pylori antibody as well as a preparation method and application thereof. The kit comprises a diluent, latex reagent and blank liquid of Helicobacter pylori antigen, a standard product and a quality control product, wherein the Helicobacter pylori antigen can be one or more of full-tropina antigen of Helicobacter pylori, urease antigen of Helicobacter pylori, cytotoxin-related protein A antigen of Helicobacter pylori or cell vacuole toxin A antigen of Helicobacter pylori; the latex reagent is a mixture of two latex granules which have different particle sizes and are adsorbed by Helicobacter pylori; and the Helicobacter pylori antibody for preparing the standard product and the quality control product is derived from IgM (immunoglobulin M) and / or IgG (immunoglobulin G) in serum of a patient infected by Helicobacter pylori. The kit is suitable for various full-automatic biochemical analyzers and semiautomatic biochemical analyzers, and has the advantages of rapid detection, high sensitivity, strong specifically, good accuracy and the like.

The invention relates to a kit for assaying NGAL in serum, and provides an NGAL assay kit suitable for a full-automatic biochemical analyzer and a special protein analyzer. The technical scheme includes that the NGAL assay kit comprises reagent R1, reagent R2 and a calibrator, wherein the reagent R1 is composed of a buffer solution, an accelerator, a surfactant and the balance purified water; the reagent R2 is composed of a buffer solution and latex microspheres combined with anti-human NGAL antibodies; and the calibrator is composed of a buffer solution, a stabilizer, a preservative, a certain amount of pure recombinant human NGAL required by concentration, and the balance purified water. The NGAL content in the serum can be quickly assayed through the reagent combination.

The invention provides a latex enhanced immunoturbidimetry NGAL detection kit. The latex enhanced immunoturbidimetry NGAL detection kit comprises a reagent R1, a reagent R2 and a standard substance; the reagent R1 comprises a buffer solution, a surfactant, a chelating agent, an accelerator and an antiseptic; the reagent R2 comprises the buffer solution, NGAL antibody-coated latex particles, and an unrelated protein; and the standard substance is a solution containing 6 NGAL recombinant protein concentration gradients. Compared with kits in the prior art, the neutral granulocyte gelatinase related lipid transporter content detection kit used on a fully automatic biochemical analyzer has the advantages of rapid determination of the content of the NGAL in urine or blood plasma, guarantee of the sensitivity and the linearity, and improved sensitivity, accuracy and linearity of the determination result.

The invention discloses a neutrophil gelatinase associated lipocalin (NGAL) chemiluminescence detection kit. The kit comprises the components of a standard substance of NGAL, an NGAL monoclonal antibody labeled by horseradish peroxidase, magnetic particles coated by the NGAL monoclonal antibody, a white non-transparent microwell plate, washing liquid, and chemiluminescence substrates A and B. The kit provided by the invention can be used for detecting the content of the NGAL in blood serum of a patient, and has important guiding significance to the detections on premature kidney diseases and injures. Compared with the conventional ELISA (Enzyme Linked Immunosorbent Assay) method, the NGAL chemiluminescence detection kit provided by the invention has the characteristics of being high in sensitivity and good in specificity and overcoming the large influence of immunoturbidimetry by blood fat concentration, and has the advantages of high stability, reliability and accuracy, security and environment friendliness, simplicity and convenience in operation and the like.

The invention relates to a kit for determining troponin-T (TNT) content of serum, aims to overcome the deficiencies of the above background technology, and provides a kit for detecting troponin-T by an immunoturbidimetry method, wherein the kit does not require sample dilution, has simple operation, high accuracy and good repeatability, and is suitable for various types of fully automatic biochemical analyzers and special protein instruments. A technical scheme is as below: the kit comprises: A. a reagent R1 containing a buffer solution, a preservative, an accelerating agent, inorganic salts, a surfactant and the balance of purified water; b. a reagent R2 containing a buffer solution, latex microspheres with diameter of 60-150 nm combined with anti-human troponin antibodies and an antiseptic; and c. a reference calibrator containing a buffer solution, a stabilizer, a preservative, a certain amount of recombinant human protein troponin-T pure product determined by concentration requirements and the balance of purified water.

The invention provides a kit for determination of apolipoprotein C2 by using immunoturbidimetry. A reagent R1 contains 10 to 500 mM of a buffer solution, 1 to 100 g / L of a surfactant, 5 to 50 g / L of an electrolyte, 2 to 100 g / L of a high-molecular accelerator accelerator, 1 to 50 g / L of a stabilizing agent and 1 to 10 g / L of an antiseptic; a reagent R2 comprises 10 to 500 mM of the buffer solution, 150 to 300 g / L of an anti-human APOC2 antibody, 5 to 50 g / L of the electrolyte, and 1 to 10 g / L of the antiseptic; and a calibrator comprises 10 to 500 mM of the buffer solution, 0 to 100 g / L of an APOC2 antigen, 1 to 50 g / L of the stabilizing agent, 1 to 10 g / L of the antiseptic and 0.01 to 10 g / L of an anti-oxidant. The kit provided by the invention is easy and fast to use, can satisfy clinical requirements for rapid high flux detection of a sample and has the advantages of obviously improved detection efficiency, small batch difference and stable reagents.

The invention relates to a preparation method of latex particles coated with a prostate specific antigen-antibody (PSA) and a PSA-enhanced turbidimetric immunophelometry kit. The latex particles coated with the PSA antibody are prepared by bonding the PSA antibody with latex particles by an optimized physical adsorption method. According to the invention, the kit utilizes the PSA antibody bonded onto the surface of the latex particles and the PSA in the human blood sample to conduct immunoreaction to form the turbidity, and the kit detects the content of the prostate specific antigen-antibody by the increase of the turbidity.

There is a demand for improved turbidimetric immunoassays for human Cystatin C in biological samples, especially in human clinical samples of body fluids. The present invention provides a turbidimetric immunoassay method and reagent set enabling measurement of human Cystatin C by turbidimetric methods, resulting in a surprisingly stronger and faster turbidimetric signal than in the present state of the art. The increased and faster signal is accomplished by the use of new reagents and compositions, and enables shorter assay times and kinetic reading with a stronger signal, improving overall assay speed and quality. Improved robustness to lipid interference and improved linearity is achieved.

The invention relates to the technical field of biology and concretely discloses a kit for detecting a helicobacter pylori antibody by using a latex immunoturbidimetry assay. The kit provided by the invention comprises a helicobacter pylori antibody calibrator, a buffer solution with the pH value of 6.5-8.5 and a helicobacter pylori gene recombination antigen latex reagent. The kit provided by the invention is high in sensitivity up to 0.10mu g / ml due to the adoption of the latex immunoturbidimetry assay for detecting the content of helicobacter pylori antibodies in a detected sample, good in stability, simple and rapid in operation, strong in specificity, difficult to be interfered, accurate in quantifying and wide in application prospect.

The invention provides an agent kit for measuring urinary transferrin by latex-enhanced immunoturbidimetry and a preparation method thereof. The agent kit is characterized by comprising emulsion particles, transferrin antibody and buffer liquid; according to appropriate paths for covalent bond between the transferrin antibody and the emulsion particles, a turbidity reaction system between 340-700 nm detection is established below the low-concentration transferrin level and the transferrin antibody bonded with the emulsion particles. The preparation method comprises the following steps of: activation of emulsion particles; bonding of the transferrin antibody and the emulsion particles; and storing. The agent kit can be used for detecting the urinary transferrin level with the concentration to be 0-12 mg / L in the urine; the accuracy can trace back to CRM470, and the precision is smaller than 10% so as to satisfy the clinic requirements.

The invention provides a creatine kinase isozyme detection kit. The kit is based on colloidal gold immunoturbidimetry, and comprises reagents R2, and the reagents R2 are solutions of gold nanoparticles marked with creatine kinase isozyme antibodies. The kit is characterized in that the particle diameter of the gold nanoparticles is 62.2 nm to 79.1 nm, and the mass ratio of the gold nanoparticles and the antibodies is 50:20-60. The invention further provides a preparation method of the kit. The kit has the advantages of being high in sensitivity, strong in specificity, fast in response and good in stability, no sediment is generated after a reaction, a biochemical analyzer is convenient to clean, and the service life of the biochemical analyzer is prolonged.