36 results about "Immunoturbidimetry" patented technology

The invention discloses a kit for determining oxidized low-density lipoprotein based on latex enhanced immunoturbidimetry and application, and belongs to the technical field of biological detection reagents, the kit comprises a reaction buffer solution and a latex reagent, the reaction buffer solution is marked as R1, and the latex reagent is marked as R2; the reaction buffer solution comprises 3-10 g / L of a buffer solution, 5-15 g / L of inorganic salt, 10-30 g / L of a turbidity increasing agent, 0.5-2.5 mL / L of a surfactant, 1-3 mL / L of a preservative and 3-14 g / L of a stabilizer; the latex reagents are at least two latex reagents formed by coupling monoclonal antibodies and latex microspheres, and the particle size range of the latex microspheres is 200-280 nm. The latex is marked by adopting two monoclonal antibodies with relatively strong specificity, so that the specificity is relatively strong under the condition of keeping the sensitivity. According to the kit, the detection sensitivity and specificity can be improved at the same time, so that the linear range of the kit is wider, the accuracy is higher, and clinical requirements are better met.

A method, device and computer readable medium for identifying hook effect in immunoturbidimetry. The method generates a reaction curve by measuring optical signals of an immune reaction of a to-be-measured substance in a sample within a predetermined time period, and determines whether the sample has hook effect by using distribution information of the reaction curve within the predetermined time period. The method only uses measurement information of a small predetermined time period in the entire reaction time to identify samples with hook effect, and terminates the detection in time, thereby accelerating the speed of immunoturbidimetry detection of samples with hook effect.

There is a need to quantify both low and high concentrations of target substances with high sensitivity. [Solution] The present invention provides an immunoturbidimetric reagent comprising a first particle and a second particle, wherein the ratio of the volume-average particle size (Dv2) of the second particle to the volume-average particle size (Dv1) of the first particle, Dv2 / Dv1, is 0.37 or more and 0.86 or less, the volume-average particle size of the second particle is 150 nm or more, and the ratio of the refractive index (R2) of the second particle to the refractive index (R1) of the first particle, R2 / R1, is 0.87 or more and 0.97 or less.

The present disclosure relates to a kit for detecting a soluble growth stimulation expressed gene 2 protein. In particular, the present disclosure relates to a latex-enhanced turbidimetric immunoassay kit for detecting the concentration and / or content of the sST2 in human samples. The kit can be used in transmission immunoturbidimetry and scattering immunoturbidimetry. The kit comprises a buffer system, an anti-interference component, latex microspheres, an anti-sST2 antibody, etc. The latex-enhanced immunoturbidimetric agent of the present disclosure can detect sST2 proteins within a range of <400 ng / ml in a sample, with a sensitivity of up to 0.1 ng / ml and a high specificity, accuracy and precision. The kit is suitable for a fully automatic biochemical analyzer and a scattering analyzer, and has the advantages of convenient and fast use and low cost, and can be used clinically to detect the sST2 protein.

The invention belongs to the technical field of biological detection, and particularly relates to a detection method and a detection kit for oxidized low-density lipoprotein. The invention provides a novel application of quaternary ammonium salt chitosan in a kit, and particularly relates to application of quaternary ammonium salt chitosan with different molecular weights in an immunoturbidimetry detection process of oxidized low-density lipoprotein. When high-molecular-weight quaternized chitosan with the molecular weight of 100-200 kDa and low-molecular-weight quaternized chitosan with the molecular weight of 30-50 kDa are used as components of the oxidized low-density lipoprotein detection kit, the quaternized chitosan with different molecular weights can react with citrate radicals to form gel particles with different mechanical strengths; the content of the oxidized low-density lipoprotein can be detected through immunoturbidimetry, and the sensitivity and the linear range of the oxidized low-density lipoprotein detection kit are greatly improved and widened by using the quaternized chitosan with different molecular weights.

The application provides a lipoprotein phospholipase A2 determination kit for latex immunoturbidimetry, which contains reagent R1 and reagent R2; the reagent R1 comprises the following components: buffer, inorganic salt, anti-interference additive, surfactant and Proclin 300; the reagent R2 comprises the following components: buffer, lipoprotein phospholipase A2 antibody coated latex microspheres, stabilizer, surfactant and Proclin 300. The kit is a liquid kit with strong stability, high accuracy, wide linear range and strong anti-HOOK capability.

The invention provides a cTnI protein antibody. The cTnI protein antibody comprises an anti-human cTnI protein monoclonal antibody I, an anti-human cTnI protein monoclonal antibody II and / or an anti-human cTnI protein monoclonal antibody III, the antibody respectively comprises a light chain and a heavy chain; wherein the light chain sequence of the antibody I is as shown in SEQ ID NO.1, and the heavy chain sequence of the antibody I is as shown in SEQ ID NO.2; a light chain sequence of the antibody II is as shown in SEQ ID NO.3, and a heavy chain sequence of the antibody II is as shown in SEQ ID NO.4; the light chain sequence of the antibody III is as shown in SEQ ID NO.5, and the heavy chain sequence of the antibody III is as shown in SEQ ID NO.6. The invention also provides a latex enhanced immunoturbidimetric kit for quantitatively detecting the cTnI protein and a preparation method of the latex enhanced immunoturbidimetric kit. The invention has the advantages that the antibody affinity is high, the specificity is good, and a kit based on the antibody is high in sensitivity, good in specificity and strong in anti-interference capability.

The invention discloses a latex enhanced immunoturbidimetry detection reagent for glycosylated hemoglobin and a use method of the latex enhanced immunoturbidimetry detection reagent, and relates to the technical field of medical examination. The latex enhanced immunoturbidimetry detection reagent for glycosylated hemoglobin comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises first nano microspheres; and the reagent R2 comprises a second nano microsphere coated with a monoclonal antibody of HbA1c. According to the reagent R2, the nano microspheres are coated with the monoclonal antibody of HbA1c, the stability risk caused by the fact that balance needs to be established for immunoreaction of multiple antibodies and antigens is avoided through the monoclonal antibody, the problem that a glycosylated hemoglobin immunoturbidimetry detection reagent is insufficient in stability can be solved, the nano microspheres are arranged on the reagent R2, and therefore the stability of the glycosylated hemoglobin immunoturbidimetry detection reagent is improved, and the stability of the glycosylated hemoglobin immunoturbidimetry detection reagent is improved. By utilizing the characteristic that the volume of an antigen-antibody conjugate can be increased after the nano-microspheres are combined with the HbA1c monoclonal antibody, the turbidimetric degree of latex is effectively amplified, and the detection sensitivity can be improved.

The invention relates to an S-4FB glucosamine modified carboxyl microsphere for latex immunoturbidimetry, a synthesis method and application. On the basis of carboxyl polystyrene microspheres, the S-4FB-glucosamine modified microspheres for latex immunoturbidimetric determination are obtained through glucosamine modification and S-4FB modification, the prepared S-4FB-glucosamine modified carboxyl microspheres have active groups and can be directly coupled with biomolecules with amino groups, operation is easy, convenient and efficient, coupling efficiency is high, and the S-4FB-glucosamine modified carboxyl microspheres have good application prospects. The anti-interference capability is strong, and the stability is good. When the S-4FB-glucosamine modified carboxyl microspheres are used as indicating microspheres for preparing a detection reagent, the repeatability, linearity, acceleration stability and kit correlation of the reagent are superior to those of unmodified carboxyl polystyrene microspheres. Therefore, the S-4FB-glucosamine modified carboxyl microspheres disclosed by the invention have a wide application prospect in the aspect of in-vitro detection.

The application provides a latex immunoturbidimetric detection reagent, a kit and a detection method. The latex immunoturbidimetric detection reagent comprises a buffer and an ionic liquid, the ionic liquid comprises an ionic liquid containing an imidazole structure, the ionic liquid containing the imidazole structure comprises 1-alkyl-3-methyl imidazole halide or 1-alkyl-3-methyl imidazole fluoride, the halogen in the 1-alkyl-3-methyl imidazole halide comprises chlorine, bromine or iodine, and the alkyl in the 1-alkyl-3-methyl imidazole halide or 1-alkyl-3-methyl imidazole fluoride comprises alkyl containing 2, 3, 4, 5, 6 or 7 C atoms. The application can solve the problem of a small linear range in the prior art latex turbidimetric method, and is suitable for the field of immunoturbidimetric detection.

The invention discloses a serum COVID-19 NAb quantitative detection reagent as well as preparation and application thereof, and belongs to the technical field of biomedical detection. The latex microsphere reagent contained in the quantitative detection reagent is produced by adopting a continuous process, so that complicated purification processes such as centrifugation and washing are avoided, the preparation time is remarkably shortened, and the complexity of the production process and the difference between batches are reduced. Meanwhile, by optimizing a buffer solution system and reaction conditions, the detection sensitivity and the linear range are improved, the linear range is 122.46 ng / mL to 10000 ng / mL, the correlation coefficient R2 is equal to 0.9999, the absolute relative deviation does not exceed + / -1.88%, the detection requirements of neutralizing antibodies (NAb) with different concentrations in clinical samples can be met, and the defect that the linear range of a traditional method is narrow is overcome. The reagent is based on an immunoturbidimetry principle, the detection process can be carried out on a full-automatic biochemical analyzer, the operation is simple and convenient, and the detection is efficient.

The application discloses an anti-dextran single-chain antibody and application. The amino acid sequence of the anti-dextran single-chain antibody is shown as SEQ ID NO. 6, and further, a COMP sequence is added at the C terminal of the single-chain antibody to form a derivative variant A48-COMP, and the amino acid sequence of the derivative variant of the single-chain antibody is shown as SEQ ID NO. 8. The derivative variant A48-COMP can produce turbidity with dextran, and can be directly applied to an immune turbidimetry method for measuring dextran. Compared with a general immune turbidimetry method, the application uses a genetic engineering antibody, is easy to prepare, has low detection cost, and has the advantages of simplicity and rapidness.

The invention discloses a reagent, a kit and a detection method for free light chain detection, and relates to the technical field of detection kits, the reagent comprises the following components: a solid phase carrier surface marked with an F (ab ') 2 antibody fragment; the liquid-phase reaction liquid comprises a composite blocking agent for inhibiting non-specific reaction of endogenous interferents; wherein the F (ab ') 2 antibody fragment and the composite blocking agent act together to improve the detection specificity and reaction linearity. The problems that non-specific reaction is caused by an antibody Fc segment and an endogenous interferent when a latex enhanced immunoturbidimetric assay is used for detecting free light chains, existing means cannot eradicate the non-specific reaction, detection linearity is abnormal, and specificity is low are solved, the solid phase carrier marked with the F (ab ') 2 antibody segment and the composite blocking agent are used, and the solid phase carrier marked with the F (ab') 2 antibody segment and the composite blocking agent have a synergistic effect; closed-loop protection is formed from two dimensions of eliminating self interference of the antibody and shielding exogenous interference of the sample, and compared with an antibody optimization or interference shielding means, the combination targeting and detection specificity of a target analyte are synergistically improved.

This invention belongs to the field of bioassay technology, and specifically relates to a method and kit for detecting oxidized low-density lipoprotein (ODL). This invention provides a novel application of quaternized chitosan in the kit, specifically using quaternized chitosan of different molecular weights in the immunoturbidimetric detection of ODL. Results show that when high molecular weight quaternized chitosan (100-200 kDa) and low molecular weight quaternized chitosan (30-50 kDa) are used as components of the ODL detection kit, the different molecular weights of quaternized chitosan react with citrate to form gel particles of varying mechanical strengths. This allows for the detection of ODL content via immunoturbidimetry. Furthermore, the use of quaternized chitosan of different molecular weights significantly improves the sensitivity and linear range of the ODL detection kit.

This invention relates to the field of immunoassay technology, specifically to a kit, preparation method, and detection method for determining p2PSA based on latex-enhanced immunoturbidimetric assay. The kit includes: a reaction solution and a latex antibody reagent; the reaction solution includes at least one of a buffer, inorganic salt, sensitizer, surfactant, blocking agent, and preservative; the latex antibody reagent includes a mixture of a first latex antibody reagent and a second latex antibody reagent; the first latex antibody reagent is formed by coupling a polyclonal antibody of p2PSA with large-particle latex microspheres; the particle size range of the large-particle latex microspheres is 172–405 nm; the second latex antibody reagent is formed by coupling a monoclonal antibody of p2PSA with small-particle latex microspheres; the particle size range of the small-particle latex microspheres is 71–121 nm; the monoclonal antibody of p2PSA includes three different monoclonal antibodies of p2PSA; the kit of this invention can ensure sensitivity in determining p2PSA while broadening the linearity and enhancing specificity.

The application relates to a free light chain, a quality control product and application thereof, the free light chain comprising one or both of a free kappa light chain and a free lambda light chain; wherein the free kappa light chain has an amino acid sequence as shown in SEQ ID NO. 1 or an amino acid sequence with at least 90 percent identity thereof; and the free lambda light chain has an amino acid sequence as shown in SEQ ID NO. 2 or an amino acid sequence with at least 90 percent identity thereof. The quality control product prepared by using the free light chain of the application can be used for quality control of various detection methods such as immunofixation electrophoresis, immunoturbidimetry and mass spectrometry detection, so as to guarantee the accuracy of clinical related disease diagnosis.

Provided are: particles for immunonephelometry, the particles containing a metal oxide and being used for highly sensitive detection of a target substance; a reagent; a test kit; and a detection method.　The particles for immunonephelometry contain a metal oxide, and are characterized in that: the refractive index of the particles is 1.70-2.25; the volume-average particle size of the particles is 200-400 nm; and the product of the refractive index and the volume-average particle size is 340-780 nm.

The invention belongs to the technical field of medical examination, and particularly relates to an oriented antibody polymer as well as a preparation method, a detection reagent and application thereof. The invention firstly provides a directed antibody polymer which is simple to prepare and efficient in reaction, and the multivalence effect of the polymerized antibody is suitable for a biochemical reagent for immunoturbidimetry. When applied to a glycosylated hemoglobin detection reagent, the glycosylated hemoglobin antibody can avoid the use of a secondary antibody, reduces unstable factors, has outstanding detection sensitivity, long linear range and good thermal stability, remarkably improves the detection performance compared with a conventional biochemical detection method, and has extremely high clinical application value.

The invention belongs to the technical field of immunodetection analysis, and discloses a kit for quantitative detection of a thrombin-antithrombin III compound and a preparation method of the kit. The kit comprises a magnetic bead working solution, an acridinium ester marker working solution, a biotin marker working solution, a TAT calibrator and a TAT quality control product, and quantitative detection of TAT is realized by combining a double-antibody sandwich method with a chemiluminescence technology. The method overcomes the defects that an existing fluorescence immunoassay method is low in repeatability, and an immunoturbidimetry method is high in requirement for specimens and limited in low-concentration detection capacity, and has the advantages of being high in specificity, sensitivity and accuracy and easy, convenient and rapid to operate. The method can be used for clinical auxiliary diagnosis of thrombogenic diseases such as disseminated intravascular coagulation (DIC) and venous thromboembolism (VTE), and provides a basis for blood coagulation state evaluation and clinical decision making.

The invention provides a soluble growth stimulation expression gene 2 protein determination kit, a preparation method and application, and belongs to the technical field of biochemical immunoassay. The latex enhanced immunoturbidimetry is adopted to detect the soluble growth stimulation expression gene 2 protein, key components of the reagent 1 and the reagent 2 are optimized and adjusted, preferred components and concentrations of polyanions, a surfactant, an accelerant, a biochemical cross-linking agent and the like are obtained, and the problems that sST2 related reagents are poor in stability and poor in stability are solved. In order to solve the technical problems that in the prior art, the detection accuracy of the high value of sST2 larger than 400 ng / mL cannot be met and the like, the detection accuracy of the high value can be remarkably improved by the optimized components and concentration in the linear range of 5 ng / mL to 432 ng / mL, particularly, the sensitivity, the anti-interference capability, the stability, the repeatability and the front zone of the correspondingly prepared sST2 latex reagent reach and are superior to those of a third-party reagent, and the detection accuracy of the high value of sST2 larger than 400 ng / mL can be remarkably improved by the optimized components and concentration in the linear range of 5 ng / mL to 432 ng / mL. And the requirements of clinical use can be well met.

The invention discloses a soluble transferrin receptor determination kit based on latex immunoturbidimetry, which is composed of the following components: the kit contains a reagent R1 and a reagent R2, the reagent R1 is prepared from the following components: a buffer solution, inorganic salt, a reaction enhancer, a surfactant and Proclin300; the reagent R2 is prepared from the following components: a buffer solution, latex particles coated with soluble transferrin receptor antibodies, a stabilizer, a surfactant and Proclin300. The invention also discloses a preparation method of the kit. The kit is high in stability, high in accuracy, wide in linear range and high in sensitivity.

This invention relates to the field of microfluidic chip technology and provides a microfluidic chip for latex immunoturbidimetric detection and its usage method. The method is as follows: A sample is added, the chip is placed on the detection platform, the diluent sac is opened, and the motor is started to rotate, allowing the sample to enter the sample quantitative sac and the diluent to enter the diluent quantitative sac. The motor stops rotating, and siphon channels one and two are opened. The motor starts rotating again, allowing the sample in the sample quantitative sac to enter the mixing sac, and the diluent in the diluent quantitative sac to enter the mixing sac, where the two are diluted and mixed. The motor stops rotating, siphon channel three is opened, and the sample mixture sequentially enters detection well one and detection well two. This invention enables the detection of the tested sample on a microfluidic chip, requiring only a fraction of the sample volume and reaction reagent volume of traditional large-scale biochemical assays, significantly shortening the detection time. Furthermore, it can improve both the reactivity of low values ​​and the resistance to hooking.

Provided are a particle for immunoturbidimetry, a reagent, a test kit, and a method for detecting a target substance, each of which can detect a trace component in a low concentration region and improve storage stability. The particle for immunoturbidimetry is a particle for immunoturbidimetry including a first resin and titanium oxide, wherein the particle for immunoturbidimetry includes a first layer containing the first resin and a second layer containing the titanium oxide, wherein the second layer is arranged on the outside of the first layer, and wherein the density of the titanium oxide is 3.40 g / cm 3 Hereinafter, and wherein the content of the titanium oxide in the particle for immunoturbidimetry is 10 mass% or more and 80 mass% or less.

The application relates to biotechnology and particularly discloses a fibrinolysin degradation product FDP antibody and application thereof. For the first time, a single B cell antibody preparation technology and a flow cytometry sorting method are applied to the development of a fibrinolysis system detection antibody in a blood coagulation in-vitro diagnosis, a mammalian cell expression system is adopted, three strains (FDP-1, FDP-2 and FDP-3) of FDP monoclonal antibodies with biological activity are successfully expressed and obtained. On an ELISA platform, the three strains of FDP recombinant rabbit monoclonal antibodies have specific reactions with primary fibrinolysis (X and Y) and secondary fibrinolysis (DD); on an immunoturbidimetry platform, FDP-1 has specific reactions with primary fibrinolysis and secondary fibrinolysis, and FDP-2 has a specific reaction with only primary fibrinolysis on the immunoturbidimetry platform. The three strains of FDP antibodies provided by the application can make up for the vacancy of domestic FDP antibodies on the market, guarantee the adaptability of D-dimer and FDP combined monitoring, and realize the integrity of fibrinolysis system detection.

This invention discloses a method for reducing the influence of lipemia on transmission-scattering fusion-enhanced immunoturbidimetric assays, specifically including the following steps: S1, measuring the average absorbance signal value of a specific measurement point after adding reaction buffer to each sample; S2, measuring the non-lipemia turbidity value of different samples that does not cause a low scattering measurement after adding reaction buffer, i.e., the background; S3, comparing the value measured in step S1 with the value measured in step S2 to determine whether the scattering turbidity measurement results need to be revised; This invention relates to the field of transmission-scattering fusion-enhanced immunoturbidimetric assay technology. This method for reducing the influence of lipemia on transmission-scattering fusion-enhanced immunoturbidimetric assays achieves this by demonstrating a strong correlation between the turbidity of lipid particles in lipemia samples and the transmission turbidity signal value in the reaction buffer during transmission-scattering fusion-enhanced immunoturbidimetric assays, and by quantifying this correlation through the transmission absorbance values ​​of the sample and R1.

This invention discloses the application of sodium thiocyanate in the preparation of a kit for coagulation assays. The kit detects coagulation parameters based on coagulation methods, chromogenic substrate methods, or immunoturbidimetric assays. These coagulation parameters include prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, fibrin(ogen) degradation products, D-dimer, antithrombin III, and anti-Xa. By adding sodium thiocyanate to the coagulation assay kit, this invention optimizes the clinical relevance of the assay kit by adjusting reaction time (coagulation method), improving the linear range (chromogenic substrate method), and enhancing the differentiation between low and high values ​​(immunoturbidimetric assay), thereby improving the accuracy of the test.

The application discloses a method and equipment for detecting oxidized low-density lipoprotein based on an immune turbidimetry method, and the method comprises the following steps: preparing a macromolecular initiator by adopting an active esterification reaction, and preparing a block graft copolymer by means of an atom transfer radical polymerization and a radical grafting reaction; the surface of different particle size latex microspheres is modified by using the graft copolymer, then the microspheres with different particle sizes are coupled with different OxLDL monoclonal antibodies respectively, so as to prepare double-particle-size latex microsphere markers; a serum sample is mixed with the double-particle-size latex microsphere markers, and an absorbance value is measured, and the concentration of oxidized low-density lipoprotein in the serum sample is calculated according to a standard curve established based on a standard sample solution. Through the scheme, the stability of the latex microsphere marker can be enhanced, and the binding efficiency of the latex microsphere marker and ox-LDL is improved.

The invention discloses a kit for improving detection specificity of latex immunoturbidimetry, and belongs to the technical field of immunodetection. The invention provides a composite buffer system for improving the detection specificity of latex immunoturbidimetry. The composite buffer system comprises the following components in working concentration: 5-50g / L of a sealing agent, 0.1-5mL / L of a nonionic surfactant and a specific interferent blocking agent. The invention further provides a high-specificity detection kit for the protein markers in body fluid or blood. The high-specificity detection kit comprises a reagent 1, a reagent 2, a standard substance and the composite buffer system. According to the invention, various components with different action mechanisms are creatively and scientifically matched to form a synergistic systematic solution, so that the problems of poor detection specificity and accuracy of latex immunoturbidimetry in the prior art are solved.

The invention relates to the technical field of monoclonal antibodies, in particular to an adiponectin ADP monoclonal antibody and application, the heavy chain sequence of the adiponectin ADP monoclonal antibody is shown as SEQ ID NO: 1, and the light chain sequence of the adiponectin ADP monoclonal antibody is shown as SEQ ID NO: 2. The adiponectin ADP monoclonal antibody disclosed by the invention has excellent specificity and sensitivity, can provide accurate and reliable reagent guarantee for related detection scenes, can be applied to a plurality of detection fields such as biochemical immunoturbidimetry, immunochromatography and ELISA (Enzyme-Linked Immunosorbent Assay), and realizes rapid, efficient and high-sensitivity clinical detection on an ADP antigen in serum.

The invention relates to a free light chain, a quality control product and application of the free light chain. The free light chain comprises one or two of a free kappa light chain and a free lambda light chain; wherein the free kappa light chain has an amino acid sequence as shown in SEQ ID NO.1 or an amino acid sequence having at least 90% identity with the free kappa light chain; the free lambda light chain has an amino acid sequence as shown in SEQ ID NO.2 or an amino acid sequence with at least 90% identity with the free lambda light chain. The quality control product prepared from the free light chain can be used for quality control of various detection methods such as an immunofixation electrophoresis method, an immunoturbidimetric method and mass spectrometric detection, and the accuracy of diagnosis of clinical related diseases is guaranteed.