9 results about "Immunoturbidimetry" patented technology

There is a need to quantify both low and high concentrations of target substances with high sensitivity. [Solution] The present invention provides an immunoturbidimetric reagent comprising a first particle and a second particle, wherein the ratio of the volume-average particle size (Dv2) of the second particle to the volume-average particle size (Dv1) of the first particle, Dv2 / Dv1, is 0.37 or more and 0.86 or less, the volume-average particle size of the second particle is 150 nm or more, and the ratio of the refractive index (R2) of the second particle to the refractive index (R1) of the first particle, R2 / R1, is 0.87 or more and 0.97 or less.

The application provides a lipoprotein phospholipase A2 determination kit for latex immunoturbidimetry, which contains reagent R1 and reagent R2; the reagent R1 comprises the following components: buffer, inorganic salt, anti-interference additive, surfactant and Proclin 300; the reagent R2 comprises the following components: buffer, lipoprotein phospholipase A2 antibody coated latex microspheres, stabilizer, surfactant and Proclin 300. The kit is a liquid kit with strong stability, high accuracy, wide linear range and strong anti-HOOK capability.

This invention belongs to the field of bioassay technology, and specifically relates to a method and kit for detecting oxidized low-density lipoprotein (ODL). This invention provides a novel application of quaternized chitosan in the kit, specifically using quaternized chitosan of different molecular weights in the immunoturbidimetric detection of ODL. Results show that when high molecular weight quaternized chitosan (100-200 kDa) and low molecular weight quaternized chitosan (30-50 kDa) are used as components of the ODL detection kit, the different molecular weights of quaternized chitosan react with citrate to form gel particles of varying mechanical strengths. This allows for the detection of ODL content via immunoturbidimetry. Furthermore, the use of quaternized chitosan of different molecular weights significantly improves the sensitivity and linear range of the ODL detection kit.

This invention relates to the field of immunoassay technology, specifically to a kit, preparation method, and detection method for determining p2PSA based on latex-enhanced immunoturbidimetric assay. The kit includes: a reaction solution and a latex antibody reagent; the reaction solution includes at least one of a buffer, inorganic salt, sensitizer, surfactant, blocking agent, and preservative; the latex antibody reagent includes a mixture of a first latex antibody reagent and a second latex antibody reagent; the first latex antibody reagent is formed by coupling a polyclonal antibody of p2PSA with large-particle latex microspheres; the particle size range of the large-particle latex microspheres is 172–405 nm; the second latex antibody reagent is formed by coupling a monoclonal antibody of p2PSA with small-particle latex microspheres; the particle size range of the small-particle latex microspheres is 71–121 nm; the monoclonal antibody of p2PSA includes three different monoclonal antibodies of p2PSA; the kit of this invention can ensure sensitivity in determining p2PSA while broadening the linearity and enhancing specificity.

Provided are: particles for immunonephelometry, the particles containing a metal oxide and being used for highly sensitive detection of a target substance; a reagent; a test kit; and a detection method.　The particles for immunonephelometry contain a metal oxide, and are characterized in that: the refractive index of the particles is 1.70-2.25; the volume-average particle size of the particles is 200-400 nm; and the product of the refractive index and the volume-average particle size is 340-780 nm.

Provided are a particle for immunoturbidimetry, a reagent, a test kit, and a method for detecting a target substance, each of which can detect a trace component in a low concentration region and improve storage stability. The particle for immunoturbidimetry is a particle for immunoturbidimetry including a first resin and titanium oxide, wherein the particle for immunoturbidimetry includes a first layer containing the first resin and a second layer containing the titanium oxide, wherein the second layer is arranged on the outside of the first layer, and wherein the density of the titanium oxide is 3.40 g / cm 3 Hereinafter, and wherein the content of the titanium oxide in the particle for immunoturbidimetry is 10 mass% or more and 80 mass% or less.

This invention discloses the application of sodium thiocyanate in the preparation of a kit for coagulation assays. The kit detects coagulation parameters based on coagulation methods, chromogenic substrate methods, or immunoturbidimetric assays. These coagulation parameters include prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, fibrin(ogen) degradation products, D-dimer, antithrombin III, and anti-Xa. By adding sodium thiocyanate to the coagulation assay kit, this invention optimizes the clinical relevance of the assay kit by adjusting reaction time (coagulation method), improving the linear range (chromogenic substrate method), and enhancing the differentiation between low and high values ​​(immunoturbidimetric assay), thereby improving the accuracy of the test.

This invention discloses a Mycoplasma pneumoniae antibody detection kit based on immunoturbidimetry, belonging to the field of clinical immunology testing. It includes solutions R1 and R2. Solution R1 is a dispersion of double-coupled latex microspheres coated with natural Mycoplasma pneumoniae antigens, and solution R2 is a sample diluent containing an enhancer. The test samples can be serum, plasma, or whole blood samples, and it is applicable to biochemical analyzers, protein analyzers, or combined blood cell and immunoturbidimetric analyzers. The kit prepared by this invention can be used on various instruments, has a wide range of detection targets, and can be coupled with other instruments to detect various clinically valuable blood indicators, further shortening the testing time. It solves the problems of poor accuracy, low efficiency, and limited application scenarios caused by the subjective interpretation of results, long processing time, and limited applicability of blood samples in existing technologies. It can effectively simplify the testing process and improve diagnostic efficiency.

This invention provides a method and reagents for measuring low concentrations of target substances using immunoturbidimetry. [Solution] A measurement method for measuring a target substance contained in a sample, wherein the target substance is a protein with a molecular weight of 150 kDa to 500 kDa, contained in the sample at a concentration of 0.8 ng / mL to 2.0 ng / mL, and comprises: a first mixing step of mixing the sample with a first reagent to obtain a first mixed solution; a second mixing step of mixing the first mixed solution with a second reagent containing particles immobilized with ligands that specifically bind to the target substance to obtain a second mixed solution; and a measurement step of measuring the absorbance of the second mixed solution, wherein the concentration of the sample in the second mixed solution is 4.0 volume% to 30.0 volume%, the mass ratio of particles to sample in the second mixed solution is 1:500 to 1:50, and the change in absorbance per 1.0 ng / mL of target substance in the measurement step is 0.0012 to less than 0.0100 for the sample.