70 results about "Reaction curve" patented technology

The invention provides a method for determining the concentration of an analyte in a sample comprising the steps of heating the sample and measuring the concentration of the analyte or the concentration of a species representative thereof in the sample at a predetermined point on a reaction profile by means that are substantially independent of temperature. Also provided is an electrochemical cell comprising a spacer pierced by an aperture which defines a cell wall, a first metal electrode on one side of the spacer extending over one side of the aperture, a second metal electrode on the other side of the spacer extending over the side of the aperture opposite the first electrode, means for admitting a sample to the cell volume defined between the electrodes and the cell wall, and means for heating a sample contained within the cell.

The invention provides a three-dimensional micro confocal measuring system and method utilizing the optical polarization characteristic. The invention is characterized by generating structured light with patterns by grating plates and then matching with polaroid elements and the structured light drift step and combining with the focus morphology measuring principle to extract a series of optical images corresponding to different depth relative to an object, then utilizing the focusing index values of a plurality of pixels in each acquired optical image to form focusing reaction curves of the pixels and finally computing the peak value of the focusing reaction curve of each pixel and carrying out recombination according to the depth corresponding each peak value to acquire the morphology of the object.

A sample analyzer optically measures reaction of a sample mixed with reagent, and obtains optical information therefrom; generates a reaction curve representing change in the optical information over time; determines a first area prior to an evaluation target time (t0) and a second area after the evaluation target time (t0) wherein the first and second areas are formed between a baseline which is parallel to the time axis and a reaction curve from a first time (t1) prior to the optional evaluation target time (t0) to a second time (t2) after the evaluation target time, and determines the reaction end point based on the first and second areas; and obtains a characteristic of a sample based on the determined reaction end point, is disclosed a sample analyzing method is also disclosed.

The embodiment of the invention relates to a calibration data correction method and electronic equipment thereof. The calibration data correction method comprises the following steps that: receiving an original dataset formed by multiple pieces of reaction data; through linear fitting, carrying out primary screening on the original dataset to obtain a primary-screening dataset; and through nonlinear fitting, carrying out secondary screening on the primary-screening dataset to obtain a corrected dataset. By use of the method, an original reaction curve dataset is subjected to coarse screening and fine screening in sequence, two fitting ways are used for iteratively removing and rejecting exceptional data which does not conform to a reaction tendency and a normal reaction rule, and the accuracy of a final calibration result is guaranteed.

The invention provides a method for identifying paddy rice kind high temperature resistance performance, comprising steps of adopting a phytotron to perform multi-gradient temperature processing during whole the paddy rice flowering period, performing fertilization rate counting on the paddy rice which is processed by various temperatures, and adopting the paddy rice high temperature comprehensive evaluation index to evaluate the high temperature resistance performance of the paddy rice type. The method disclosed by the invention can obtain the high temperature reaction curves of the fertility periods of various types, accurately reflects the real high temperature performance of the paddy rice type and the paddy rice breeding material; diurnal change configuration of each temperature processing simulates the typical regional high temperature weather condition, which enables the environment configuration to be more scientific; and the invention adopts the phytotron as a high temperature processing device, which enables the processing condition to be stable and reliable and enables the result to be good in repetition.

A determination method for a hydration rate curve of electrical steel dedicated magnesium oxide belongs to the detection field of the chemical properties of a compound. A hydration rate is recorded by a method of hydrating first and then roasting magnesium oxide; and a reaction curve is mapped by taking the hydration time of the magnesium oxide as a horizontal coordinate and the hydration rate as a vertical coordinate, so as to be used for analyzing the hydration performance of the magnesium oxide. The determination method has the advantages that the operation is simple and convenient, the result is accurate, and the determination method can visually reflect the moisture which can be brought into a furnace by magnesium oxide in the production, and the determination method is well joined with the production.

The invention discloses a method for controlling input amount of a tap water flocculating agent. The method comprises the steps of obtaining a feedforwad predicted value for the input amount of the flocculating agent through multi-factor feedforward learning prediction of an artificial nerve network, then testing a correspondence curve between flocculating agent adding lag time and water source flow through a reaction curve method, and recording corresponding data every certain time period; searching data which correspond with a (K-1) time point and a (K-2) time point which are related with a K time point, and obtaining three sets of mutually related data; and applying three sets of obtained data into a PID control algorithm, thereby forming a large-lag PID control algorithm for adjusting the feedforward predicted value, and realizing automatic control for the input amount of the tap water flocculating agent. According to the method of the invention, adjustment calculation is performed on the feedforward predicted value through the large-lag PID control algorithm, thereby greatly improving accuracy of the input amount of the flocculating agent, improving control precision for water turbidity before filtering, reducing control residual error, ensuring safe production and smooth operation in a water industry, and realizing energy saving, consumption reduction, personnel number reduction and benefit increase.

The invention relates to a reaction curve generation method. The reaction curve generation method comprises the following steps: 1) acquiring a transmission light signal value to obtain initial time sequence light quantity data and form an initial curve; 2) selecting a function model meeting the initial curve and fitting according to the initial curve to obtain a fitting curve; 3) comparing whether a determination coefficient of the fitting curve is no less than a target value or not; if so, entering step 5); otherwise, entering step 4); 4) calculating theoretical time sequence light quantitydata and determining repairing data; reforming an initial curve and returning back to step 2); 5) taking the fitting curve as a reaction curve. The reaction curve formed by the method disclosed by theinvention similarly restores a reaction process at maximum so that an absorbance value calculated by the reaction curve is more accurate. The invention also relates to a reaction curve generation device and an optical detection system.

The invention discloses a method for quickly and specifically detecting the enzymatic activity of a phospholipid-depending factor X activator. A chromogenic substrate assay is adopted, a microplate reader is used, a linear reaction curve is obtained in a certain reaction time and a certain-activity standard substance range, a linear relation between a light absorption value of a product and the concentration of the phospholipid-depending factor X activator is established, and the corresponding enzymatic activity of a sample is calculated. In addition, the recovery rate and accuracy of the standard curve are carefully researched. The method is high in accuracy, simple in steps and convenient to impellent in a laboratory, and is suitable for quality control of X activator raw materials and hemocoagulase preparations.

The invention relates to an enzymatic activity test method using combining integration and initial velocity method, which is characterized in that the classical initial velocity is measured when the concentration of initial substrate is 70% higher than that of the preset substrate, and the classical initial velocity is still within linearity range; when the substrate consumption proportion in 80% record period is higher than the lower limit required in integration, the fitting reaction curve of integral velocity equation is used to determine the maximum reaction velocity using reaction time as independent variable, then the maximum reaction velocity is calculated as the initial velocity with preset substrate concentration is 93%; meanwhile, the slope of classical initial velocity and computation initial velocity to the enzyme amount reaction curve; when the substrate consumption proportion of the coupling enzyme reaction system in 80% record period is higher than the lower limit required in integration, the fitting enzyme reaction curve is used to determine the initial velocity using corresponding numerical integral velocity equation, otherwise the classical initial velocity is directly measured; total time for the optimization monitoring reaction guarantees the combination of the two methods.

The invention relates to a protein suspension chip of ricin and methods for preparing, quantifying and detecting the same. The detecting method comprises the following steps: adding a working solution and a sample to be tested into a hole; adding a detection antibody; adding SA-PE; adding a detection buffer solution and mixing the mixture evenly; and reading numerical values, analyzing data and judging the detection result. The quantifying method comprises the following steps: adding a positive test sample or a standard product for dilution through gradient multiple proportions, detecting a series of diluted samples in a suspension chip system, and reading a corresponding fluorescence value (MFI); making a dosage-reaction curve; and fitting the dosage-reaction curve and an equation. The suspension chip comprises a coded microsphere, a ricin capturing antibody coated on the microsphere, a biotin-marked ricin detection antibody, a streptavidin-phycoerythrin and the buffer solution. The methods have the advantages of good detectability, high sensitivity, strong specificity and wide dynamic range.

There is provided a technique for automatically determining or predicting a line range specific to a sample that appears in a reaction curve in an automated analyzer for mixing a specimen and a reagent and measuring a change in a mixture of the specimen and the reagent with time. This invention approximates reaction curve data by a function and automatically determines a curve part at an early stage or a second stage of a reaction. The invention determines a line range not including a curve part for each sample and calculates a laboratory test value using absorbance data within the determined line range. This invention also automatically determines a start time of line at the early stage of the reaction on the basis of absorbance data obtained up to a point halfway through the reaction curve, predicts a line range on the basis of the end time of line and a planned end time of line, and calculates a predictive value on the basis of a result of the prediction.

The present invention provides assays and methods of compensating for changes in the dose-response curve of an assay where such changes are due to variations in a perturbing variable such as, but not limited to temperature. This is achieved by a two-step method, the first step of which involves measurements of the dose-response curve, and thus the individual assay parameters, at many different values of the perturbing variable, spanning the expected range of the perturbing variable. In the second step, unknown samples are assayed simultaneously with a known standard at a chosen analyte concentration. During this measurement, the value of the perturbing variable is unknown and the dose-response curve is therefore also unknown. The different dose-response curves from the first step are used to determine a mathematical relationship between the assay parameters and the assay signal of the known standard. With this relationship, in which the value of the perturbing variable is implicit rather than explicit, assay parameters that are valid for the unknown value of the perturbing variable can be obtained by substituting the value of the assay signal from the known standard (measured when assaying the unknown samples) into the mathematical relationship and solving for the assay parameters. The method enables an accurate determination of the analyte concentration even when the perturbing variable is changing or fluctuating from one sample measurement to another. Once the first step is completed, the second step can be performed repeatedly to measure unknown samples with accuracy.

The method for quantitative determination of biochemical substance by means of predetermining background and making enzyme analysis includes the following steps: adding buffer solution and tool enzyme in the sample solution to be determined, measuring the change of light absorption with reaction time, and utilizing integral velocity equation to fit and analyze the negative curve of light adsorption consumed by substrate to predetermine background instead of directly detemining background. It can directly determine body fluid sample, and it has no meed of diluting said sample, and its determination accuracy is high.

The invention relates to a beta-fructofuranosidase enzyme-linked immunoassay detection kit which comprises a beta-fructofuranosidase standard enzyme solution, an enzyme label plate enveloped with a beta-fructofuranosidase monoclonal antibody, a sample diluent, a beta-fructofuranosidase rabbit polyclonal antibody working solution, an enzyme labelled secondary antibody working solution, a substrate coloured solution, a washing concentrate and a stop solution. For the kit disclosed by the invention, the content of beta-fructofuranosidase in a honey sample is measured by adopting a double antibodies sandwich ELISA (enzyme-linked immuno sorbent assay) method; a linearly dependent coefficient of a dosage-reaction curve is more than 0.980; R2 is equal to 0.9993; the sensitivity is 10U/KG; and the national ELISA detection quality requirement is met; and an intra-plate variation coefficient (CV%) of the kit is less than 10 percent. The kit has stable measurement result and good precision. The kit disclosed by the invention has the advantages of simpleness in processing the sample, high detection speed, short detection time of only 2.5 hours, sensitivity, accuracy and high throughput, and provides an effective means for detecting counterfeiting of honey.

The invention discloses a composite foul gas sensor detecting device which comprises a gas inlet unit, a sampling pool unit and a processing unit. The gas inlet unit is connected with the sampling pool unit which is connected with the processing unit. The gas inlet unit comprises two gas storage cylinders, a mass flow meter and a three-way valve. The sampling pool unit comprises at least one gas sampling module, a diaphragm pump, a sampling pool unit shielding wire and a power line. The gas inlet unit is used for providing detected gas with stable flow velocity and background gas with a flushing effect and ensuring the rapid switching of the detected gas and the background gas. A sensor for detecting foul gas and a signal conditioning circuit board matched with the sensor are put in the sampling pool unit so that complex foul components can be extracted and analyzed. The processing unit further processes signals collected by the sensor so that a gas reaction curve can be obtained and finally evaluates the concentration level of foul gas.

The invention discloses a whole blood CRP detection apparatus, which comprises a sampling unit, a reagent unit, a reagent distribution unit, a red blood cell detection unit, a white blood cell detection unit, a CRP detection unit, a signal processing and acquisition unit, a storage and calculation unit and an output unit, wherein the signal processing and acquisition unit comprises a red blood cell signal processing and acquisition module, a white blood cell signal processing and acquisition module and a CRP signal processing and acquisition module, and the storage and calculation unit is usedfor performing calculation on acquired red blood cell pulse waveform data and acquired white blood cell pulse waveform data to obtain packed cell volume and performing calculation on first CRP reaction signal data and second CRP reaction signal data to obtain a CRP reaction curve, and finally calculating according to the CRP reaction curve and the packed cell volume to obtain a CRP value. With the whole blood CRP detection apparatus of the present invention, in the case of the accurate measurement of the low-value CRP sample, the upper limit of the measurement range of the CRP can be improved, and the inaccurate measurement result of the high-value CRP sample can be avoided.

The invention relates to a reagent for determining total bilirubin by a sodium nitrite oxidation method, which can effectively solve present problems and has a wide linear range, strong interference immunity, a standard reaction curve, a rapidly-reached end point, high accuracy and precision of determination, and the capability of practical application without environmental pollution. The preparation method of the reagent is that: a reagent 1 is prepared by adding water into 2.25-5.26 g of trisodium citrate, 16.40-19.80 g of citric acid, 0.10-1.0 ml of polyethyleneglycol octyl phenyl ether, 0.20-0.65 g of disodium ethylene diamine tetraacetate to obtain 1000 ml, and mixing the mixture, wherein the reagent 1 has a pH of 2.2-3.6; a reagent 2 is prepared by adding water into 0.14-0.56 g of sodium nitrite, 0.05-0.15 g of sodium hydroxide, 0.10-0.30 g of sodium carbonate to obtain 1000 ml, and mixing the mixture, wherein the reagent 2 has a pH of 11-13. The invention has scientific components, is easy to produce, has low cost, stable performance, and long shelf life, is accurate in determination, and has no environmental pollution.

The invention relates to a method for measuring enzyme substrate amount by combining a terminal balance method and an enzyme reaction dynamic process to process enzyme reaction process data. The method comprises the steps: the data, which has the characteristic that the signal change between adjacent data exceeds two times of the noise of an instrument, in a continuously recorded enzyme reaction curve is valid data; detection signals are measured before little enzyme liquid is added, and an enzyme liquid dilution effect is corrected to obtain a corrected starting signal; when the number of the valid data in the enzyme reaction curve is not more than 7 or the absolute value of the difference between the corrected starting signal and the final recorded data is smaller than 50 times of the noise of the instrument, a reaction terminal signal is determined by the terminal balance method, and when the number of the valid data is more than 7 or the absolute value of the difference between the corrected starting signal and the final recorded data is larger than 50 times of the noise of the instrument and the consumption ratio of the substrate achieves requirement, the reaction terminal signal is predicted by an enzyme reaction process analysis method; and the concentration of the substrate to be measured is determined according to a standard response curve with the absolute value of the difference between the corrected starting signal and the reaction terminal signal as a measuring index. The method is applicable to irreversible enzyme reaction systems which can be continuously monitored.