The invention provides a diluent capable of improving the stability of an acridinium ester antigen-antibody conjugate and reducing the background. The diluent comprises the components of 5 to 7g of Tris-base, 3.5 to 4.0 mL of concentrated hydrochloric acid, 9 to 11 g of polyethylene glycol-6000, 0.95 mL to 1.05 mL of 10% lauryl sodium sulfate, 9.5 mL to 10.5 mL of Tween, 0.8 to 1.0 g of ethylene diamine tetraacetic acid disodium salt, 9 to 11 g of sodium caseinate, 0.95 mL to 1.05 mL of Proclin 300, 9.5 to 10.5 mL of triton X-100, and 1000mL of purified water for an in-vitro diagnostic reagent. The diluent has the advantages that the components are few; polyethylene glycol 6000, sodium dodecyl sulfate, tween and several active agents are mixed for use, meanwhile, a Tris-base buffer solution system is used, and multiple raw materials play a mutual synergistic effect, so that the diluent has a better protection effect on acridinium ester labeled antigens and antibodies, can be stored for a long time, can provide good blocking property and protectiveness for the antibodies, and reduces non-specific reactions; meanwhile, the material cost is saved, the specificity, stability, repeatability and other properties of the reagent are not influenced, and the reagent is suitable for popularization and application.