23 results about "Proclin" patented technology

The invention discloses a triiodothyronine dissociation agent, a detection kit, a preparation method of the triiodothyronine dissociation agent and application of the triiodothyronine dissociation agent and the detection kit, and the triiodothyronine dissociation agent comprises 10-100 mM of acetic acid-sodium acetate buffer solution, 0.05-1 g / L of ethylene diamine tetraacetic acid disodium salt, 1-10 g / L of perfluoroalkyl ethyl methacrylate, 0.2-1 mL / L of Tween-20 and 0.2-2 mL / L of ProClin 300, the pH (Potential of Hydrogen) of the triiodothyronine dissociation agent is 5.5 + / -1; the kit comprises a triiodothyronine dissociation agent and a reagent strip. According to the present invention, the two sites can be specifically recognized, the sensitivity and the specificity are high, the high-specificity detection on the specific antigen can be achieved, and the low-end sensitivity is high; meanwhile, the interference problem of rare earth europium during detection of triiodothyronine can be solved, and the accuracy of a detection result is further improved.

The invention relates to a chemiluminescence kit for detecting pepsinogen II. The kit comprises an enzyme marker, a luminescent substrate solution and an anti-interference buffer system, the anti-interference buffer system comprises a nonionic surfactant, animal-derived IgG (immunoglobulin G), an antioxidant and catalase; the chemiluminescence kit also comprises a PG II calibration product and a PG II quality control product. The core active component of the enzyme marker is beta-galactosidase. The enzyme marker further comprises an anti-PG II monoclonal antibody marked by beta-galactosidase, bovine serum albumin, trehalose, cane sugar, MgCl2. 6H2O, sodium chloride, Proclin300 and a phosphate buffer solution. According to the kit, a brand-new anti-interference buffer system is introduced on the basis of a spatial proximity chemiluminescence analysis method, so that the resistance to clinically common interferents (such as hemolysis, lipemia, jaundice and heterotropism antibodies) is remarkably improved, and the detection result is more accurate and reliable.

The application relates to a buffer reagent, a kit, a branched-chain alpha-keto acid or a branched-chain amino acid detection method in the technical field of biological detection. The buffer reagent comprises 15-25 mM, pH 8.5-9.5 Tris-HCl buffer solution, 10-50 g / L trehalose, 0.1-1.5 g / L Proclin 300, 0.1-0.5 g / L Triton X-100, 0.1-1.5 g / L sodium salt of EDTA, 1-10 kU / L ascorbic acid oxidase and 0.5-3 g / L bovine serum albumin. The buffer reagent is suitable for preparation of a reagent containing leucine dehydrogenase, the reagent containing the buffer reagent and the leucine dehydrogenase and the kit prepared by using the reagent can effectively eliminate interference problems in the process of being used for branched-chain alpha-keto acid, branched-chain amino acid detection, and has good correlation with mass spectrometry.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention provides a composite quality control product of an AIDS antibody, a syphilis antibody and a hepatitis C antibody as well as a preparation method and application of the composite quality control product. The composite quality control product comprises an AIDS (Acquired Immune Deficiency Syndrome) antibody, a syphilis antibody, a hepatitis C antibody and the following components: a matrix solution, 1.5 to 3 g / L of Pluronic F68, 0.05 to 0.15% v / v of ProClin 300, 0.1 to 1 g / L of sodium surfactin, 1 to 10 g / L of bovine serum albumin, 1 g / L of alanine, 2 to 5 g / L of trehalose, 0.5 to 1.0 g / L of EDTA (Ethylene Diamine Tetraacetic Acid) and 2 to 5 mmol / L of benzamidine. The matrix liquid comprises a buffer solution and 20-30% v / v human serum. According to the invention, the aggregation and inactivation risk of the antibody in the freezing and thawing process is effectively reduced, the antibody can be repeatedly frozen and thawed for more than three times, the degradation of the antibody at high temperature can be effectively prevented, and the reliability of the quality control product in the transportation and clinical use process is ensured.

The invention relates to the technical field of biological detection, and discloses a sample diluent and application thereof and a kit, pure water is taken as a solvent, and the sample diluent at least comprises 15-80 g / L of soluble sodium salt, 0.05-0.5 g / L of soluble potassium salt, 8-15 g / L of amino acid, 0.2-1 g / L of goat serum albumin, 0.2-1 g / L of surfactant, 0.5-2 g / L of trehalose and 0.5-2 mL / L of Proclin-300 according to concentration, the sample diluent provided by the invention can weaken non-specific binding of impurities in a blood sample and a target analyte, so that the accuracy of a final detection result is improved.

The application relates to the technical field of biological detection, and discloses a sample diluent, application and kit thereof, wherein the solvent is pure water and at least includes 15-80 g / L of soluble sodium salt, 0.05-0.5 g / L of soluble potassium salt, 8-15 g / L of amino acid, 0.2-1 g / L of goat serum albumin, 0.2-1 g / L of surfactant, 0.5-2 g / L of trehalose and 0.5-2 mL / L of Proclin-300; the amino acid is an alkaline amino acid; and the sample diluent provided by the application can weaken the non-specific combination of impurities and target analytes in a blood sample, thereby improving the accuracy of a final detection result.

The invention belongs to the technical field of medical immunodiagnostic reagents, and discloses a freeze-dried VB12 rapid detection kit and a VB12 detection method. According to the kit provided by the invention, in the preparation process of a freeze-dried microsphere detection reagent, trehalose, glucose, gelatin, triton, polyethylene glycol, Proclin 950, 5-bromo-5-nitro-1, 3-dioxane, mannitol and glycine in a specific mass ratio are adopted as a freeze-drying solution system; stress damage to VB12 binding protein and a VB12 compound antibody in the freeze-drying process is reduced, the stability of the reagent in the preparation and storage process is improved, meanwhile, quick redissolution of the freeze-drying reagent can be achieved, and a detection system formed by compounding a reagent storage solution, a freeze-drying microsphere redissolution solution and a sample dissociation solution has the excellent anti-interference capacity and can be used for detecting the VB12 binding protein and the VB12 compound antibody in the freeze-drying process. The obtained detection kit has the advantages of excellent detection sensitivity, reliability, stability and the like, and has a very excellent practical application prospect.

The invention discloses a sampling extracting solution for a total IgE antibody detection product, a total IgE antibody detection kit and application of the total IgE antibody detection kit, and the sampling extracting solution comprises the following components in percentage by mass: 0.5-2% of a component A, 0.5-2% of a component B and the balance of a component C, 0.3% to 1.2% of Tris; 0.3% to 1.2% of Na2HPO4; 0.3% to 1.2% of NaCl; 0.01%-0.1% of a component B; the balance of purified water; wherein the component A is Triton X100 or Tween 20, and the component B is Tween 20; and the component A is prolin 300 or NaN3, and the component B is prolin 300 or NaN3. According to the present invention, with the extraction liquid, the detected substance can be completely and efficiently extracted from the sampling test paper, and the components of the extraction liquid are matched with the reaction system of the reagent so as to improve the antigen-antibody binding efficiency to a certain extent; soft materials such as sampling filter paper are adopted to suck ocular surface liquid, so that hard materials can be prevented from directly making contact with the surfaces of eyeballs, substance exchange between other liquid and the human body is avoided, a subject can conduct sampling by himself / herself, the risk can be remarkably reduced in the sampling process, operation is easy, and professionals do not need to cooperate for operation.

The invention discloses a detection kit for detecting calprotectin in human excrement, the detection kit is composed of a sample diluent and detection test paper, the detection test paper is composed of a bottom plate, a nitrocellulose membrane, a conjugate pad, a sample pad and absorbent paper, the absorbent paper, the nitrocellulose membrane, the conjugate pad and the sample pad are sequentially overlapped on the bottom plate, a shell is arranged outside the detection test paper, and the shell is connected with the sample pad. An observation window and a sample adding hole are formed in the shell, and the position of the observation window is matched with the position of the nitrocellulose membrane; the position of the sample adding hole is matched with the position of the sample pad; the nitrocellulose membrane is sequentially provided with a T1 detection line coated with an anti-calprotectin monoclonal antibody, a T2 detection line coated with an anti-calprotectin monoclonal antibody and a quality control line C coated with a goat anti-chicken IgY antibody and / or a goat anti-mouse IgG antibody; a calprotectin monoclonal antibody marked by colloidal gold is solidified on the combination pad, and the sample diluent is prepared from sodium chloride, EDTA (Ethylene Diamine Tetraacetic Acid), PEG (Polyethylene Glycol) 20000, Proclin-300 and purified water.

The application provides a complex quality control product of HIV antibody, syphilis antibody and hepatitis C antibody and a preparation method and application thereof. The complex quality control product comprises HIV antibody, syphilis antibody and hepatitis C antibody and the following components: a matrix liquid, 1.5-3 g / L Pluronic F68, 0.05-0.15 % v / v ProClin 300, 0.1-1 g / L sodium mycobacterial lipopeptide, 1-10 g / L bovine serum albumin, 1 g / L alanine, 2-5 g / L trehalose, 0.5-1.0 g / L EDTA and 2-5 mmol / L benzamidine; the matrix liquid comprises a buffer and 20-30 % v / v human serum. The application effectively reduces the aggregation and inactivation risk of the antibodies in the freeze-thaw process, can be repeatedly freeze-thawed more than three times, can effectively prevent the degradation of the antibodies under high temperature, and ensures the reliability of the quality control product in the transportation and clinical use process.

This invention discloses a chromogenic solution and kit for visual colorimetric detection of free ferrous protoporphyrin in cells. The chromogenic solution consists of reagents A, B, and C: Reagent A is prepared with deionized water and contains NaCl, Proclin 300, and glycerol; Reagent B is prepared with an acetate-sodium acetate buffer at pH 4.0 and contains glycerol, Triton X-100, β-cyclodextrin, disodium EDTA, citric acid, ethanol, and 3,3',5,5'-tetramethylbenzidine; Reagent C is prepared with an acetate-sodium acetate buffer at pH 4.0 and contains 2.0-4.0% hydrogen peroxide and 35.0-50.0% ethanol. The chromogenic solution of this invention has a lower background value and can detect the content of free ferrous protoporphyrin in cells within 10 minutes by visual colorimetry, with a detection limit as low as 10 nmol / L. The chromogenic stability is maintained for 12 hours, making it suitable for rapid assessment of cellular pathological conditions.

The application provides a method for stable storage of pseudo-virus at room temperature, a preservative solution prepared by using BSA, Tween 80, PEG-8000, Na2HPO4, NaH2PO4, KCl and Proclin-300, 500-800 mL of purified water is taken, the above reagents are added, fully stirred until completely dissolved, the pH value is adjusted to 7.2-7.6 by using dilute hydrochloric acid or 1M NaOH, the purified water is diluted to 1000 mL, and then 0.22 mu m filter membrane is used for filtering and sterilizing, so that the pseudo-virus can be stably stored in the preservative solution at 0-37 DEG C for 12 months, the storage period of the pseudo-virus at 0-37 DEG C is greatly prolonged, the transportation and the later related application of the pseudo-virus are more favorable, and the method has the advantages of low cost and convenient use.

This invention relates to the field of biodetection technology, disclosing a preservation solution, an adiponectin detection kit, its preparation method, and its application. The preservation solution comprises Tris, trehalose, Tween 20, sodium caseinate, PVP-10, Proclin-300, TX-100, surfactant S13, and glycine. This specific formulation effectively prevents quantum dot aggregation and maintains protein activity for a long time. The adiponectin detection kit is based on quantum dot immunofluorescence chromatography technology and includes components such as a sample pad, a release pad, and a nitrocellulose membrane. The release pad is simultaneously coated with quantum dot-labeled antibodies and unlabeled detection line antibodies, and the quantum dot-labeled antibodies are coupled in a cascade manner of "quantum dot-streptavidin-biotinylated antibody". This invention, through liquid dialysis linking technology and a unique release pad antibody premixing process, utilizes the signal amplification effect of the biotin system and the principle of homogeneous reaction, significantly improving the sensitivity, accuracy, and reagent stability of the detection.

The invention provides an enzyme-labeled antibody protective agent and application of the enzyme-labeled antibody protective agent in an ELISA (Enzyme-Linked Immunosorbent Assay) detection kit. The provided enzyme-labeled antibody protective agent is prepared from 0.1 to 0.5 percent of aminated mesoporous silicon dioxide nano particles, 0.5 to 2 percent of bovine serum albumin, 5 to 9 percent of complex saccharides, 0.01 to 0.1 percent of poloxamer 188, 0.01 to 0.05 percent of reduced glutathione and 0.02 to 0.05 percent of Proclin-300. The protective agent provided by the invention can effectively maintain the structure and activity of the enzyme-labeled antibody under various adverse conditions such as temperature fluctuation, oxidative stress and repeated freezing and thawing. The protective agent has broad-spectrum stability, not only can significantly reduce the activity loss of the antibody in the storage, transportation and use processes, but also can maintain the native conformation of the enzyme and the antibody by slowing down the oxidation effect, thereby avoiding inactivation and aggregation, and significantly prolonging the storage life and the use cycle of the antibody.

The invention relates to the field of biological medicine, in particular to an antithrombin III determination kit and a preparation method thereof. The antithrombin III determination kit comprises a reagent 1, a reagent 2, an ATIII calibration product and an ATIII quality control product. The reagent 1 is prepared from a Tris-HCl buffer solution, calcium chloride, bovine serum albumin, potassium ferrocyanide, gentamicin sulfate, ProClin 300, recombinant human thrombin and a nano blocking agent; the reagent 2 is prepared from a Tris-HCl buffer solution, S-2238, gentamicin sulfate and ProClin 300; the ATIII calibrator is prepared from a synthetic plasma matrix, recombinant human ATIII, natural ATIII, ProClin 300, a blood coagulation inhibitor, protamine and trehalose; the ATIII quality control product is prepared from a synthetic plasma matrix, recombinant human ATIII, natural ATIII, ProClin 300, a blood coagulation inhibitor, trehalose and mannitol, according to the scheme, the method is suitable for a full-automatic blood coagulation analysis platform and conforms to the development trend of in-vitro diagnostic reagents.

The application belongs to the technical field of in vitro diagnostic reagents, and discloses a cleaning solution for acridinium ester chemiluminescence immunoassay of allergens, a preparation method and application thereof. The cleaning solution is composed of HEPES buffer, a triple surfactant system (poloxamer 188, sodium deoxycholate and octyldecyl glucoside), glycerol, PEG8000, disodium EDTA and a composite preservative of Proclin 950 and BHT. The triple surfactant synergistic system is adopted to reduce non-specific adsorption, the HEPES weak alkaline buffer system avoids the inhibition of phosphates on acridinium ester luminescence, and the composite preservative system solves the toxicity and stability problems of traditional preservatives. The cleaning solution can significantly reduce the background luminescence value, the inhibition rate of hemolysis / lipemia / jaundice interference is less than 5%, the detection accuracy deviation is within ±5% (r>0.99), the precision is less than 3.5%, and the room temperature stability is more than 13 months, and the comprehensive performance is better than that of the commercially available products.

The application provides a cell climbing sheet processing reagent for a Ranvier node / paranode antibody spectrum detection kit (cellular immunofluorescence method), a method and application thereof, and relates to the technical field of in-vitro detection. The cell climbing sheet processing reagent comprises a cell fixing solution with a specific composition and an antigen repair composition after cell fixing, wherein the cell fixing solution is composed of paraformaldehyde, formaldehyde, acetic acid, raffinose and a buffer solution, and the antigen repair composition after cell fixing is composed of ammonium sulfate, hydroxylamine hydrochloride, Brij 78, TCEP, ProClin 300 and a buffer solution. Through the synergistic effect of the two, the natural conformation of the target antigen is maximally retained, and non-specific interference is reduced, so that precise and efficient detection of the Ranvier node / paranode antibody spectrum NF155, NF186, CNTN1, CASPR1 and MAG can be realized, the clinical diagnosis demand is met, and reliable technical support is provided for precise typing of autoimmune Ranvier node disease.

The invention belongs to the technical field of biology, and particularly relates to an ELISA (enzyme-linked immuno sorbent assay) kit composition and kit for detecting advanced oxidized protein products. The kit composition is prepared from the following raw materials: 20 to 50 mM of Tris-HCl, 100 to 200 mM of NaCl, 0.5 to 2.0 percent (w / v) of fetal calf serum, 0.1 to 0.5 percent (w / v) of sodium caseinate, 1 to 5 mM of disodium ethylene diamine tetraacetate, 1 to 5 percent (w / v) of trehalose, 0.05 to 0.2 percent (w / v) of polyvinylpyrrolidone and 0.02 to 0.05 percent (v / v) of ProCin 300. The kit composition provided by the invention is applied to the kit, so that the kit has better accuracy, sensitivity and stability.

The application provides a composite buffer and application and products thereof in Helicobacter pylori typing, and relates to the technical field of immunodiagnosis. The composite buffer provided by the application comprises Tris-HCl, NaCl, bovine serum albumin (BSA), polyethylene glycol nonylphenyl ether (NP40), methyl isothiazolinone (MIT), glycine, perfluorooctanoic acid and Proclin 300. The composite buffer provided by the application is combined with a chemiluminescence determination technology to detect Helicobacter pylori, and has the advantages of high sensitivity, good repeatability and strong anti-interference capability.

The present application relates to the technical field of biology, and particularly relates to a colloidal gold goat anti-mouse secondary antibody storage buffer.The present application discloses a colloidal gold goat anti-mouse secondary antibody storage buffer, which is prepared by mixing the following components: bovine serum albumin, glycerol, an acid buffer, a pH regulator, ammonium sulfate, dextran, Proclin 300 and goat anti-mouse secondary antibody.The present application has the advantages of simple buffer formula, easy access to raw materials, easy amplification, and improved reactivity and stability of the goat anti-mouse secondary antibody without affecting the performance of the chromatographic test strip.

The invention relates to the technical field of in-vitro diagnosis, and discloses a preparation method of full-soluble animal plasma, which comprises the following steps: S1, preparation: taking animal whole blood, mixing an anticoagulant and the animal whole blood according to a volume ratio of 1: 8-1: 10, and immediately and uniformly mixing to obtain anticoagulant whole blood; the anticoagulant is a 3.2%-3.8% sodium citrate aqueous solution or a composite anticoagulant containing sodium citrate and / or citric acid; s2, cold bath treatment; s3, transportation: transporting the anticoagulant whole blood in a heat preservation box at 2-8 DEG C; s4, standing is carried out; s5, siphon transfer: carrying out siphon transfer on the supernatant liquid; s6, centrifugation: performing graded centrifugation and collecting supernatant; and S7, preparation: taking the high-speed centrifugal supernatant, and adding at least one of Proclin300, potassium sorbate, sodium diacetate and benzalkonium bromide to obtain the traditional Chinese medicine composition. The animal plasma prepared through the technical scheme is free of hemolysis and insoluble particles, and the in-batch variation coefficient of CV: R, K, Angle and MA is smaller than 8%; and the inter-batch relative range: the inter-batch relative range of R, K, Angle and MA is less than 10%.

The invention relates to a chemiluminescence kit for detecting pepsinogen I. The chemiluminescence kit comprises an enzyme marker, a luminescent substrate solution and an anti-interference buffer system, the anti-interference buffer system comprises a nonionic surfactant, animal-derived IgG (immunoglobulin G), an antioxidant and catalase; the chemiluminescence kit also comprises a PGI calibration product and a PGI quality control product. The core active component of the enzyme marker is beta-galactosidase. The enzyme marker further comprises an anti-PGI monoclonal antibody marked by beta-galactosidase, bovine serum albumin, trehalose, cane sugar, magnesium chloride, sodium chloride, Proclin300 and PBS (Phosphate Buffer Solution). According to the kit, on the basis of the advantages of a spatial proximity method luminescence analysis method, a brand new anti-interference buffer system is introduced, so that the resistance of the kit to clinical common sample mechanism effects (such as hemolysis, lipemia, jaundice and heterotropism antibodies) is remarkably improved, and a detection result is more accurate and reliable in a complex real clinical sample.