10 results about "Proclin" patented technology

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention belongs to the technical field of medical immunodiagnostic reagents, and discloses a freeze-dried VB12 rapid detection kit and a VB12 detection method. According to the kit provided by the invention, in the preparation process of a freeze-dried microsphere detection reagent, trehalose, glucose, gelatin, triton, polyethylene glycol, Proclin 950, 5-bromo-5-nitro-1, 3-dioxane, mannitol and glycine in a specific mass ratio are adopted as a freeze-drying solution system; stress damage to VB12 binding protein and a VB12 compound antibody in the freeze-drying process is reduced, the stability of the reagent in the preparation and storage process is improved, meanwhile, quick redissolution of the freeze-drying reagent can be achieved, and a detection system formed by compounding a reagent storage solution, a freeze-drying microsphere redissolution solution and a sample dissociation solution has the excellent anti-interference capacity and can be used for detecting the VB12 binding protein and the VB12 compound antibody in the freeze-drying process. The obtained detection kit has the advantages of excellent detection sensitivity, reliability, stability and the like, and has a very excellent practical application prospect.

The invention discloses a sampling extracting solution for a total IgE antibody detection product, a total IgE antibody detection kit and application of the total IgE antibody detection kit, and the sampling extracting solution comprises the following components in percentage by mass: 0.5-2% of a component A, 0.5-2% of a component B and the balance of a component C, 0.3% to 1.2% of Tris; 0.3% to 1.2% of Na2HPO4; 0.3% to 1.2% of NaCl; 0.01%-0.1% of a component B; the balance of purified water; wherein the component A is Triton X100 or Tween 20, and the component B is Tween 20; and the component A is prolin 300 or NaN3, and the component B is prolin 300 or NaN3. According to the present invention, with the extraction liquid, the detected substance can be completely and efficiently extracted from the sampling test paper, and the components of the extraction liquid are matched with the reaction system of the reagent so as to improve the antigen-antibody binding efficiency to a certain extent; soft materials such as sampling filter paper are adopted to suck ocular surface liquid, so that hard materials can be prevented from directly making contact with the surfaces of eyeballs, substance exchange between other liquid and the human body is avoided, a subject can conduct sampling by himself / herself, the risk can be remarkably reduced in the sampling process, operation is easy, and professionals do not need to cooperate for operation.

This invention discloses a chromogenic solution and kit for visual colorimetric detection of free ferrous protoporphyrin in cells. The chromogenic solution consists of reagents A, B, and C: Reagent A is prepared with deionized water and contains NaCl, Proclin 300, and glycerol; Reagent B is prepared with an acetate-sodium acetate buffer at pH 4.0 and contains glycerol, Triton X-100, β-cyclodextrin, disodium EDTA, citric acid, ethanol, and 3,3',5,5'-tetramethylbenzidine; Reagent C is prepared with an acetate-sodium acetate buffer at pH 4.0 and contains 2.0-4.0% hydrogen peroxide and 35.0-50.0% ethanol. The chromogenic solution of this invention has a lower background value and can detect the content of free ferrous protoporphyrin in cells within 10 minutes by visual colorimetry, with a detection limit as low as 10 nmol / L. The chromogenic stability is maintained for 12 hours, making it suitable for rapid assessment of cellular pathological conditions.

The application provides a method for stable storage of pseudo-virus at room temperature, a preservative solution prepared by using BSA, Tween 80, PEG-8000, Na2HPO4, NaH2PO4, KCl and Proclin-300, 500-800 mL of purified water is taken, the above reagents are added, fully stirred until completely dissolved, the pH value is adjusted to 7.2-7.6 by using dilute hydrochloric acid or 1M NaOH, the purified water is diluted to 1000 mL, and then 0.22 mu m filter membrane is used for filtering and sterilizing, so that the pseudo-virus can be stably stored in the preservative solution at 0-37 DEG C for 12 months, the storage period of the pseudo-virus at 0-37 DEG C is greatly prolonged, the transportation and the later related application of the pseudo-virus are more favorable, and the method has the advantages of low cost and convenient use.

This invention relates to the field of biodetection technology, disclosing a preservation solution, an adiponectin detection kit, its preparation method, and its application. The preservation solution comprises Tris, trehalose, Tween 20, sodium caseinate, PVP-10, Proclin-300, TX-100, surfactant S13, and glycine. This specific formulation effectively prevents quantum dot aggregation and maintains protein activity for a long time. The adiponectin detection kit is based on quantum dot immunofluorescence chromatography technology and includes components such as a sample pad, a release pad, and a nitrocellulose membrane. The release pad is simultaneously coated with quantum dot-labeled antibodies and unlabeled detection line antibodies, and the quantum dot-labeled antibodies are coupled in a cascade manner of "quantum dot-streptavidin-biotinylated antibody". This invention, through liquid dialysis linking technology and a unique release pad antibody premixing process, utilizes the signal amplification effect of the biotin system and the principle of homogeneous reaction, significantly improving the sensitivity, accuracy, and reagent stability of the detection.

The application belongs to the technical field of in vitro diagnostic reagents, and discloses a cleaning solution for acridinium ester chemiluminescence immunoassay of allergens, a preparation method and application thereof. The cleaning solution is composed of HEPES buffer, a triple surfactant system (poloxamer 188, sodium deoxycholate and octyldecyl glucoside), glycerol, PEG8000, disodium EDTA and a composite preservative of Proclin 950 and BHT. The triple surfactant synergistic system is adopted to reduce non-specific adsorption, the HEPES weak alkaline buffer system avoids the inhibition of phosphates on acridinium ester luminescence, and the composite preservative system solves the toxicity and stability problems of traditional preservatives. The cleaning solution can significantly reduce the background luminescence value, the inhibition rate of hemolysis / lipemia / jaundice interference is less than 5%, the detection accuracy deviation is within ±5% (r>0.99), the precision is less than 3.5%, and the room temperature stability is more than 13 months, and the comprehensive performance is better than that of the commercially available products.

The application provides a cell climbing sheet processing reagent for a Ranvier node / paranode antibody spectrum detection kit (cellular immunofluorescence method), a method and application thereof, and relates to the technical field of in-vitro detection. The cell climbing sheet processing reagent comprises a cell fixing solution with a specific composition and an antigen repair composition after cell fixing, wherein the cell fixing solution is composed of paraformaldehyde, formaldehyde, acetic acid, raffinose and a buffer solution, and the antigen repair composition after cell fixing is composed of ammonium sulfate, hydroxylamine hydrochloride, Brij 78, TCEP, ProClin 300 and a buffer solution. Through the synergistic effect of the two, the natural conformation of the target antigen is maximally retained, and non-specific interference is reduced, so that precise and efficient detection of the Ranvier node / paranode antibody spectrum NF155, NF186, CNTN1, CASPR1 and MAG can be realized, the clinical diagnosis demand is met, and reliable technical support is provided for precise typing of autoimmune Ranvier node disease.

The invention belongs to the technical field of biology, and particularly relates to an ELISA (enzyme-linked immuno sorbent assay) kit composition and kit for detecting advanced oxidized protein products. The kit composition is prepared from the following raw materials: 20 to 50 mM of Tris-HCl, 100 to 200 mM of NaCl, 0.5 to 2.0 percent (w / v) of fetal calf serum, 0.1 to 0.5 percent (w / v) of sodium caseinate, 1 to 5 mM of disodium ethylene diamine tetraacetate, 1 to 5 percent (w / v) of trehalose, 0.05 to 0.2 percent (w / v) of polyvinylpyrrolidone and 0.02 to 0.05 percent (v / v) of ProCin 300. The kit composition provided by the invention is applied to the kit, so that the kit has better accuracy, sensitivity and stability.

The present application relates to the technical field of biology, and particularly relates to a colloidal gold goat anti-mouse secondary antibody storage buffer.The present application discloses a colloidal gold goat anti-mouse secondary antibody storage buffer, which is prepared by mixing the following components: bovine serum albumin, glycerol, an acid buffer, a pH regulator, ammonium sulfate, dextran, Proclin 300 and goat anti-mouse secondary antibody.The present application has the advantages of simple buffer formula, easy access to raw materials, easy amplification, and improved reactivity and stability of the goat anti-mouse secondary antibody without affecting the performance of the chromatographic test strip.