The present invention discloses an 
RNA virus inactivation preservation solution. The inactivation preservation solution comprises 
ammonium salt, 1-ethyl-3-methylimidazolium bisulfate, 
lithium chloride, 
ethylenediaminetetraacetic acid, an RNase inhibitor, a surfactant, a 
reducing agent, a buffer agent and water. The present invention also provides an 
RNA virus sample inactivation and preservation method and an 
RNA virus detection method, and the method is conducted by using the inactivation preservation solution. The preservation solution has low cost, inhibits RNase activity, preserves RNA integrity, realizes preservation and transportation of samples at 
room temperature, can also quickly inactivate pathogens at 
room temperature, reduces 
infection risks of medical personnel, and reduces false negatives in detection. At the same time, the inactivation preservation solution can realize a 
lysis function, does not contain chaotropic reagents and alcohols, is compatible with mainstream 
nucleic acid extraction kits on the market, greatly saves time of an entire process, and is particularly suitable for 2019 new 
coronavirus (COVID-19) inactivation and 
lysis steps after swab sampling.