110 results about "Preservation solutions" patented technology

The invention discloses a liquid cell product, a preparation method thereof and a preserving fluid for preparing the liquid cell product, and belongs to the technical field of immunodetection. The dendritic polymer is creatively added into the preserving fluid for preparing the liquid cell product, the cell morphology in the liquid cell product can be improved, and a thermal acceleration test proves that the preserving fluid can maintain the cell morphology in the liquid cell product for a long time. When the preservation solution is used for preparing the liquid cell quality control product for immunodetection, quality control spots with complete circles can be formed after sample application on slides made of different materials, it can be obviously judged that cells in the liquid cell quality control product are dispersed very uniformly after immunohistochemical staining, and the preservation solution has very high application value.

The invention relates to the technical field of organ transplantation, and discloses organ preserving fluid, application and organ dynamic supercooling perfusion equipment. The organ preserving fluid is prepared from ribose, glucose, mannitol, lidocaine, adenosine, a high-molecular polymer, L-proline, trehalose, inorganic salt and organic salt. The high-molecular polymer comprises one of hydroxyethyl starch, polyethylene glycol and albumin. According to the organ preserving fluid disclosed by the invention, the freezing point of the perfusate is reduced through the micromolecular cryoprotectant, and meanwhile, the micromolecular cryoprotectant and the high-molecular polymer jointly inhibit formation and growth of ice crystals; the problem of liquid preservation at the temperature below zero is solved from the two dimensions of physics (reducing the freezing point and inhibiting ice crystals) and biology (maintaining cell viability), and feasibility is provided for long-time low-temperature transfer and transplantation of organs. The preserving fluid can be used for static preservation or perfusion preservation of various organs such as kidney, liver, heart, pancreas, lung, small intestine, skin and the like.

The invention discloses a red blood cell room-temperature preservation solution as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The erythrocyte preservative comprises the following components in parts by weight: 1-200 parts of an anticoagulant, 1-200 parts of a blood cell stabilizer, 1-100 parts of a metabolic inhibitor, 1-100 parts of an electrolyte, 1-150 parts of an antioxidant and 350-1000 parts of deionized water, and the pH value of the erythrocyte preservative is 4.6-7.0. All the components in the preservative are mutually matched and have a synergistic effect, and the integrity of erythrocyte membranes can be kept through synergistic protection of the multiple components; the electrolyte equilibrium osmotic pressure is maintained, the antioxidant scavenges free radicals, and hemolysis is reduced; the preservation time is prolonged, and the cell morphology and function are good; the dependence on a cold chain is reduced, and the demand on deep hypothermia equipment is reduced.

The invention relates to a fresh flower preservation method which sequentially comprises the following steps: manually poking grape seeds, cleaning and drying, grinding powder to obtain grape seed powder, adding ethanol in a ratio of 1: 10, adopting a microwave soaking method to obtain a preservation solution, diluting by 50 times, and uniformly spraying on leaves by adopting a spraying method. According to the fresh flower preservation method provided by the invention, the storage time of the petals of the fresh flowers can be prolonged, the colors of the petals cannot be obviously changed after the petals are placed for a long time, meanwhile, the period of water loss and withering is also greatly prolonged, and the sales range of the fresh flowers is expanded.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention discloses a composition and a method for constructing ovarian organoid based on a clinical tissue sample. The composition comprises a sample preservation solution, a sample digestive juice and a sample culture medium, the method comprises the following operation steps: transporting a preservation solution A to a laboratory, and pretreating to obtain tissue fragment precipitates; carrying out resuspension incubation on the tissue fragment precipitate by using digestive juice B for digestion; collecting tissue blocks which are not completely digested and filtered, and performing secondary digestion to obtain single cells; combining the cells obtained in two times to obtain the required single cell; adding matrigel, then adding the mixture into a pore plate, and putting the pore plate into an incubator until the matrigel is solidified; and taking out the pore plate, adding a culture medium L2, and culturing to obtain the ovarian organ constructed based on the clinical sample. According to the method, the ovarian organoid is efficiently constructed from the ovarian tissue sample; the provided culture solution formula makes up the dilemma that normal ovarian tissue organoids are difficult to form and normal tissue-derived organoids are difficult to passage at present, and reduces the burden and experiment cost brought by sampling in the disease research process.

The utility model discloses a urine sample collection and preservation device, which belongs to the technical field of sample experiment reaction and comprises a preservation container and a collection container, the collection container is sleeved outside the preservation container and is detachably matched with the preservation container, the collection container is provided with an open collection cavity, and the preservation container is provided with a sealed storage cavity; the storage container is of a transparent structure and is provided with at least a first scale mark and a second scale mark, in the storage cavity, a first capacity space is formed between the first scale mark and the bottom of the storage container, and a second capacity space is formed between the second scale mark and the bottom of the storage container. The volume of the second capacity space is larger than that of the first capacity space. The first scale mark and the second scale mark are used as marks, the preserving fluid is quantitatively added, the urine sample and the preserving fluid are quantitatively mixed and preserved, it is ensured that the urine sample is relatively stable, cells in the urine sample are well preserved, and the accuracy of detection data is obviously improved.

The invention provides a cleaning and preserving fluid for fetal brain tissues and a preserving method thereof, and belongs to the technical field of tissue preservation. The cleaning and preserving fluid comprises trehalose, nerve growth factors, a GlutaMAX supplement, Y27632, S-nitrosoglutathione, tert-butylhydroquinone, a Versene solution and polyvinylpyrrolidone, compared with a traditional tissue preserving fluid, the cleaning and preserving fluid not only can improve the purity of neural stem cells, but also can maintain the activity and the yield of the neural stem cells in fetal brain tissue, and the cleaning and preserving fluid has the advantages that the cleaning and preserving fluid is simple in structure and convenient to use. The structural integrity of the cells is guaranteed, a high-quality sample is provided for subsequent research on proliferation, differentiation and the like of the neural stem cells, and the method has a wide application prospect.

A drug development assistance device comprising a processor, wherein the processor predicts a stable range in which the stability of a large molecule drug is equal to or higher than a set level for a first condition which is one among a plurality of conditions related to the composition of a preservation solution for the large molecule drug.

The invention provides a preservation method of mold mycelium pellets. According to a preservation solution used for low-temperature preservation, the COD concentration is 500-1000 mg / L, the NH4 < + >-N concentration is 100-300 mg / L, the PO4 < 3->-P concentration is 20-50 mg / L, the metal ion concentration is 0.5 mg / L, and the pH value is 2-3. The preservation solution for normal temperature preservation has the COD concentration of 100-300 mg / L, the NH4 < + >-N concentration of 20-50 mg / L, the PO4 < 3->-P concentration of 5-10 mg / L, the metal ion concentration of 0.2 mg / L and the tea polyphenol concentration of 1-2 g / L, and the pH value is adjusted to 2-3. With the adoption of the preserving fluid, strain cells in the mycelium pellets are kept in a low-energy state, only weak endogenous metabolism is performed, mycelium pellet cells are not split, the mycelium pellets can keep lasting activity, and the storage conditions of the mold mycelium pellets are improved, so that the utilization rate of the mycelium pellets is greatly increased.

The invention relates to the technical field of liquid-based cytology, and particularly discloses serosa effusion cell preserving fluid. Comprising a preservation solution, an anticoagulant, a red blood cell treatment solution, a cell membrane stabilizer, an antioxidant and a metal ion chelating agent, the preservation solution contains a buffer system, an energy supply component, an antibiotic and a surfactant, and the red blood cell treatment solution contains a hemolysis active component and a cell immobilization component; the preparation method comprises the following steps: firstly, preparing the preserving fluid, adjusting the pH value, adding the active substances, mixing the antibiotics, and then completing constant volume and sterile filtration; the anticoagulant solution and the red blood cell treating fluid are independently prepared, and sterilization is achieved through filtration; and finally, packaging all the components in a sterilized storage bottle in a matched manner. The composition provided by the invention can be used for preserving cells in a clinical serosal effusion sample, and can provide a high-quality sample for subsequent cell morphology observation or detection; in addition, according to the preparation method, the controllability is high when all the components are independently prepared, and the preserving fluid with the consistent performance can be stably prepared.

This invention discloses a method for preparing large-scale, original-color seaweed specimens, belonging to the field of seaweed preparation methods. The method includes the following steps: S1: Collecting algal bodies with reproductive organs and basal holdfasts; S2: Washing the algal bodies from S1 with pretreated water, selecting algal bodies with intact reproductive organs and basal holdfasts, and drying them; S3: Completely wrapping the algal bodies from S2 with plastic wrap and irradiating them with ultraviolet light; S4: Preparing a preservation solution; S5: Immersing the algal bodies from S3 in the preservation solution; removing them after 5 minutes; S6: Preparing the original-color specimen: removing the algal bodies from S5, placing them in a paper bag, and air-drying them in a dry, ventilated location. The dried specimen is then the original-color specimen. This method can produce original-color seaweed specimens.

The present application relates to a high concentration of ammonium bisulfite preservation solution, the preservation solution components include ammonium bisulfite and ethanol, the concentration of ammonium bisulfite is about 13 mol / L to about 15 mol / L, the concentration of ethanol is about 0.3 mol / L to about 1.5 mol / L. The present application also provides a method and kit for preserving high concentration of ammonium bisulfite solution.

The application relates to the technical field of medicines, in particular to a lymphocyte preservation solution, a preparation method and application in sample pretreatment, and the lymphocyte preservation solution is prepared from the following components in parts by mass: Tris-HCl buffer 90-100 parts, trehalose 10-15 parts, trichostatin A 0.01-0.03 parts, nuclease inhibitor 0.3-0.5 parts and the like raw materials. Through the synergistic design of a composite buffer system and functionalized nanomaterials, combined with multi-stage active protection technology, cell metabolism regulation and precise isolation of external interference factors are realized. The application improves the processing efficiency of complex samples, prolongs the preservation time of key biomarkers, and enhances the adaptability in extreme environments. The integration of the innovative preparation process and the intelligent quality control module provides reliable support for immune diagnosis and treatment, rare cell research and remote medical treatment, and has wide clinical application and scientific research transformation prospects.

To provide a package body in which a drug can be supported by an ophthalmic medical device during storage, and the drug is released from the ophthalmic medical device during use.SOLUTION: A package body includes an ophthalmic medical device, a drug, and a preservation solution. The ophthalmic medical device includes a hydrogel including a resin including a constitutional unit derived from a monomer including an amine structure. The pH of the preservation solution is 6.5 or less.SELECTED DRAWING: None

This invention discloses a simple long-term preservation method for small insect specimens used in DNA molecular detection, comprising the following steps: Freshly collected insect specimens are placed in centrifuge tubes filled with anhydrous ethanol, which is added to 4 / 5 of the tube's height, and a label is written on the tube before placement; insects of the same species from the same collection site are classified under a dissecting microscope and placed in the same centrifuge tube; after classification, centrifuge tubes from the same collection site are placed in the same sealed container, and a magnesium sheet wrapped in a water-permeable material is placed in the container, with anhydrous ethanol added until the magnesium sheet is submerged; the sealed container is then stored away from light, and anhydrous ethanol is added periodically. This invention solves the problem of preservation solution evaporation due to poor sealing of centrifuge tubes, and also reduces the water content of the anhydrous ethanol in the outer container, enabling long-term or permanent preservation of insect specimens, and allowing for quick identification based on label markings.

A cell preservation solution composition, excluding dimethyl sulfoxide (DMSO), includes a copolymer of compound A and compound B. In a composition of 100 wt % of the cell preservation solution composition, the compound A ranges from 60.00 wt % to 99.95 wt %, and compound B ranges from 0.05 wt % to 40.00 wt %.

The invention relates to the technical field of cell protection, and provides a cell protection solution, a protection method and application. The cell protection liquid comprises leonurine, human serum albumin and normal saline. The cell protection method comprises the following steps: S1, adding the human serum albumin into normal saline to prepare a cell preservation solution; s2, adding the leonurine into a cell preservation solution to prepare a cell protection solution; s3, resuspending and storing the collected mesenchymal stem cells or immune cells by using the cell protection liquid. The cell protection liquid is applied to mesenchymal stem cells and immune cells. According to the cell protection liquid, the protection method and the application provided by the invention, the cell activity can be maintained after mesenchymal stem cells and immune cells are prepared into a cell preparation, and the cell protection liquid has no toxic or side effect on the cells.

The present application relates to the technical field of biology, and particularly relates to a use of a cell preservation solution in preparation of an immunosuppressant. The present application provides a use of a cell preservation solution in preparation of an immunosuppressant, the cell preservation solution is obtained by a specific method, and the cell preservation solution is applied to a preparation (such as an injection solution) containing mesenchymal stem cells, so that the injection solution is endowed with certain immunosuppressive function, the combination with MSCs cells improves the treatment effect, and the long-time transportation capacity and the survival rate of MSCs are improved.

1. The name of this design product: Tissue fixator. 2. The purpose of this product design: Fixing pathological tissues can avoid the hazards of passive inhalation of formaldehyde, and can also prevent the formaldehyde preservation solution from volatilizing and polymerizing and precipitating, which requires regular renewal and replenishment. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. The bottom surface of this design product is difficult to see or cannot be seen during use, so the top view is omitted.

The invention belongs to the field of biology, and discloses a tissue formula preserving fluid containing antimycin A. The tissue formula preserving fluid comprises a basic culture medium, the basal culture medium contains superoxide dismutase, catalase and antimycin A. On the basis of the superoxide dismutase and the catalase, the preservation solution is matched with the antimycin A, so that the activity of tissue cells can be remarkably improved; in the research process, we have the following specificity that: 1, when the respiratory chain inhibitor drug is applied to the formula of the invention, the improvement degree of the cell viability of only antimycin A is the highest; 2, the function of antimycin A must be exerted based on the use of superoxide dismutase and catalase; meanwhile, the invention further provides a tissue preservation method.

Disclosed is a contact lens product, which comprises a packaging box, a sealing layer, a contact lens, and a preservation solution. The packaging box is provided with an accommodating recess body and a handheld piece body connected to the accommodating recess body. The accommodating recess body comprises a bottom wall and an annular sidewall that connects the handheld piece body and the bottom wall. The sealing layer seals an opening of the accommodating recess body, such that the sealing layer and the accommodating recess body jointly enclose to form a closed space, the closed space having a volume of 1.4 ml to 2.0 ml. The contact lens and the preservation solution are located inside of the closed space, and the contact lens is completely immersed within the preservation solution and is not exposed to air. The volume of the preservation solution is 75% to 90% the volume of the closed space. Hence, the contact lens is completely immersed within the preservation solution and is not exposed to a bubble, hence effectively preventing the contact lens from dehydration or deformation.

The invention relates to the technical field of storage, in particular to an oral cavity sample storage device which comprises a storage tube, a one-way piece, a collection rod, a sealing column and an interaction plate, and a complete technical scheme is formed through optimization of multiple embodiments. During collection, a sample is obtained through the ball head of the collection rod, and after the storage tube is placed, a preservation solution generates impact and suction through the through hole of the interaction plate by shaking, so that the sample is quickly and uniformly dissolved, and long-term soaking of the collection head is avoided. The one-way piece is designed through a buffer groove, a piston and a push rod, so that the preservation liquid is controllably discharged in a dripping mode, and overflow is prevented. The sealing column is matched with a double-horn-mouth and supporting rod structure through a sliding block-sliding groove-limiting groove, rapid positioning and limiting are achieved, sealing is enhanced, the shaking space is reduced, and the dissolving efficiency is improved. The device avoids detection impurity interference, is reliable in sealing and convenient to operate, adapts to various detection scenes and sample requirements, considers sample storage stability and detection accuracy, and greatly improves the pretreatment efficiency.

The application provides a method for improving the effect of sperm freezing preservation, and particularly relates to adding sperm into a freezing preservation solution containing carnosine to perform freezing preservation. The freezing preservation solution added with carnosine can effectively improve the activity of the sperm after freezing. The addition of carnosine into the sperm freezing preservation solution can effectively improve the quality of the sperm after freezing preservation, and provides certain technical support for sperm preservation.

This invention relates to the field of fecal genomics kit technology, and particularly to a safe, rapid, and high-throughput kit for extracting fecal genomes and its usage method. The kit includes a sample preservation solution, magnetic beads, a lysis buffer, a washing buffer 1, a washing buffer 2, and an elution buffer. The sample preservation solution is composed of guanidine isothiocyanate, sodium citrate, Triton X-100, Tween 20, propidium iodide, and purified water. The magnetic beads are hydroxyl magnetic beads. The lysis buffer is composed of guanidine hydrochloride, Tris base, Tris hydrochloric acid, Triton X-100, isopropanol, and purified water. Washing buffer 1 is composed of guanidine hydrochloride solution and isopropanol. Washing buffer 2 is composed of anhydrous ethanol and purified water. This kit can easily extract the genome from fecal samples, is simple to operate, safe and non-toxic, and can extract multiple samples at once, enabling rapid diagnosis and filling the gap in the current market for automated high-throughput kits for extracting genomes from fecal samples.

This invention discloses a preservation solution for preserving sample DNA at room temperature and its preparation method, belonging to the field of biological sample preservation technology. The preservation solution comprises: a lysis system, a nucleic acid protection system, and a buffering and stabilizing system; the lysis system comprises a complex surfactant and an enzymatic hydrolysis aid; the complex surfactant comprises: polyether polyol fatty acid ester and cocamidopropyl hydroxysulfonate betaine; the enzymatic hydrolysis aid comprises lysozyme Lyso-V and proteinase K; the nucleic acid protection system comprises: nitrogen-heterocyclic polyamine-carboxylic acid derivatives and dextran sulfate; the nitrogen-heterocyclic polyamine-carboxylic acid derivatives comprise: 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''tetraacetic acid, 1,4,7-triazacyclononane-N,N',N''-triacetic acid, disodium ethylenediaminetetraacetate-nitrocyclic derivative, and diethylenetriaminepentaacetic acid-piperazine derivative; the buffering and stabilizing system comprises: an amphoteric buffer and a polymeric stabilizer; the amphoteric buffer comprises: 2-(N-morpholino)ethanesulfonic acid and N-tris(hydroxymethyl)methylglycine.

The invention relates to the technical field of biomedical sample preservation, and discloses a preserving fluid of porcine fat-derived mesenchymal stem cell exosomes, a preparation method and application thereof. The exosome preserving fluid provided by the invention comprises the following components in percentage by weight: 5%-20% of polyvinylpyrrolidone (PVP); 2%-10% of trehalose; 0.5%-5% of glycerol; according to the cryoprotectant, a cryoprotectant is adopted, a phosphate buffer solution (PBS) is adopted as a solvent, polyvinylpyrrolidone (PVP) is adopted to replace traditional dimethyl sulfoxide (DMSO) to serve as the cryoprotectant, the problems that in the prior art, a preserving fluid is large in toxicity and poor in preserving effect are solved, and the application of the cryoprotectant in medical external application such as wound repair, inflammation treatment and skin care is expanded.

This invention discloses a method for preserving surgical transplant materials, a combination of transplant materials, and a skull transplant material, belonging to the field of biomedical engineering and surgical transplant material processing technology. The method includes the following steps: skull harvesting and pretreatment, preparation and precooling of preservation solution, pulsed xenon light sterilization, sealing of the surgical transplant material, and cryopreservation. This application employs a pulsed xenon light sterilization method for preserving surgical transplant materials, which solves the problems of chemical sterilization potentially causing residual penetration and toxicity to bone cells, high-temperature sterilization leading to collagen denaturation and cell inactivation, and irradiation sterilization potentially causing DNA damage to cells and incurring high equipment costs. This method maintains the cell activity of the transplant material after long-term cryopreservation while improving the osteogenic induction and healing quality of the transplant material, and also has low preservation costs.

The present application relates to the technical field of biology, and discloses a cell preservation solution, a preparation method and preparation thereof, and a cell preservation method. The cell preservation solution contains 1-10 mL compound electrolyte glucose injection, 1-10 mL human blood albumin injection, 1-10 mL compound amino acid injection, 10-20 uM salvianolic acid B, 20-60 uM rhodioside, and 1-10 uM ginkgo biloba bilobal, and the compound electrolyte injection is supplemented. The cell preservation solution can improve the biological activity of cells and prolong the preservation time of cells in multiple ways by adding traditional Chinese medicine extracts as antioxidants, membrane stabilizers and antithrombotics in response to the excess of free radicals in the low-temperature preservation process.

The invention discloses deep learning-based deep sea cold spring microbiome antibacterial peptide directional screening and application thereof, and belongs to the technical fields of deep sea extreme environment microbial resource high-value utilization, food preservation and fresh-keeping and bioinformatics. The antibacterial peptide DSCSAMP1-4 applicable to the soy sauce in the high-salt environment is directionally screened by constructing a data set containing polypeptide physicochemical properties, structural characteristics and weighted scores. Wherein the DSCSAMP2-3 containing the alpha-helical structure has obvious growth inhibition on gram-negative bacteria such as escherichia coli and pseudomonas aeruginosa, gram-positive bacteria such as staphylococcus aureus and fungi such as saccharomycetes respectively. The antibacterial peptide is natural, safe, wide in antibacterial spectrum, high in acting efficiency and free of chemical residues, does not affect the flavor of soy sauce, can be prepared into preservative additives of various dosage forms, provides an efficient and environment-friendly preservative solution for the soy sauce industry, promotes high-value utilization of deep sea cold spring microbial resources, and has great industrial popularization value.