708 results about "Preservation solutions" patented technology

An improved preservation solution is described, which is intended for the preservation of organs and tissues, or parts thereof, from humans and animals. The improved preservation solution contains calcium, at least one colloidosmotically active substance, and nitroglycerin. Also described is a method for preserving organs and tissues, or parts thereof, from humans and animals in the improved preservation solution.

The invention relates to the technical field of clinical medicines, and particularly relates to a cell preservation solution and a preparation method and applications thereof. The cell preservation solution comprises albumin, glucose, vitamin C and basic preservation liquid, wherein the basic preservation liquid is a mixture of compound electrolyte injection and compound amino acid injection. The cell preservation solution disclosed by the invention achieves a good maintaining effect on the viability and morphology of more than two kinds of seed cells, and can preserve the seed cells for a long time, and maintain the biological characteristics of the seed cells, thereby significantly improving the therapeutic effect of the seed cells.

A method of preserving, storing and transplanting mammalian donor organs. The method includes the cooling of refrigeration preservation, loading pre-freezer preservation, cryopreservation and washing solutions at least containing polyvinylpyrrolidone, a calcium channel blocker, a nucleoside, potassium chloride, polyethylene glycol, at least one amino acid, and a steroid to a temperature of 2° to 4° C. and/or of 0° to 2° C., harvesting a donor organ, perfusing it with one or more of the solution, immersing it in one or more of the solutions and storing it at a temperature above 0° C. or at a temperatures below 0° C. The cryopreservation solution also contains cryopreservative agents. Preserved organs may be transplanted directly from refrigeration storage or from freezer storage by cooling the washing refrigeration preservation solutions to 2° to 4° C., perfusing the organ with washing solution and then preservation solution, and transplanting it.

The invention relates to a pathological examination, in particular to a liquid-based cell preservation solution used in cytopathological examination of human cervical mucus, sputum, urine, pleural fluid, tracheal mucus and the like. It is characterized in that it is prepared from alcohols, sodium phosphate buffer, edetate disodium, sodium chloride 0.08%-0.12%, potassium chloride, formaldehyde, dithiothreitol, calcium acetate, magnesium acetate, etc. As a result, it can not only maintain the stability of the cell structure. It can reduce the agglomeration and precipitation of cell mucus and the loss of cell rupture, and can also make cells easy to stain, improve the clarity of cell preparation, facilitate the smooth progress of pathological examination, and the cost is low, which is conducive to popularization and use.

To provide an aqueous solution for cell preservation which is free of a natural animal-derived component such as a basal medium or serum. An aqueous preservation solution showing a high cell survival rate was obtained by removing a natural animal-derived component such as a basal medium or serum and controlling other components and their concentrations.

The current invention provides a new organ preservation solution, suitable for machine perfusion, for maintaining viability of organs, parts of organs and tissues. This solution has been designed to overcome a number of problems associated with hypothermic machine perfusion of donor organs, in particular organs obtained from non-heart-beating donors. The solution prevents or minimizes the adverse affects caused by ischemia, hypoxia, energy and nutrient depletion, acidification, hypothermia and reperfusion injury. The preservation solutions according to the current invention are superior to current state of the art preservation solutions, in particular for preservation and perfusion of organs obtained from non-heart-bearing donors, by supplying increased concentrations and an optimized balance of amino acids, vitamins, anti-oxidants, high molecular weight additives and enhanced buffering capacity. In addition, the preservation solution according to the invention combines optimal physical and chemical properties with the use of readily available, inexpensive and pharmaceutically tested and acceptable compounds, reducing the cost of manufacturing and facilitating medical certification of solutions according to the current invention.

The invention relates to a preservation solution for organs, tissues or cells of human bodies or animals. The organ preservation solution comprises sodium citrate, potassium citrate, magnesium sulfate, sodium dihydrogen phosphate, sodium hydroxide, adenosine, arginine, tryptophan, mannitol, tetramethylpyrazine hydrochloride and other components. The organ preservation solution can be used for cooling, lavaging and preserving the organs of the human bodies or the animals, can effectively preserve human in vitro kidneys for 48 hours and animal in vitro kidneys for 72 hours, can prevent ischemia-reperfusion injury, and has great value for clinical application.

The invention relates to the technical field of litchi preservation, and particularly discloses a room-temperature litchi storage preservative, and a preparation method and an application of the preservative. The preservative mainly comprises carboxymethyl-chitosan, nisin, natamycin, tea polyphenol, epsilon-polylysine, citric acid and phytic acid; litchi fruit is soaked in a preservation solution, and then aired, so that the litchi fruit can be stored at a room temperature; the quality and low level browning of the litchi fruit can be kept for a long time at the room temperature. Litchi treated by the preservative has the characteristics that the preservative is good in preservation effect, safe, environment-friendly, simple to operate and convenient to use; the preservative is extensive in source, low in cost, edible, and easy to degrade; no pesticide residue risk exists; short-term preservation during room-temperature storage and room-temperature logistics can be achieved; the preservative is a novel preservation material with a great development potential.

To provide a cell preservation solution that enables cells to be preserved not only in cryopreservation but also in cold storage, and improves the performance of cell preservation, and also provides a simple cell preservation method using the preservation solution. The cell preservation solution is characterized in that it contains at least carbohydrates, sodium ions and potassium ions, and the molar concentration of the sodium ions is equal to or greater than the molar concentration of the potassium ions. In this way, the cells can be preserved not only by cryopreservation but also by cryopreservation, thereby providing a convenient cell preservation method. Furthermore, with the cell preservation solution of the present invention, cells can be suspended in the preservation solution and directly inoculated in a culture medium for cell culture without going through steps such as washing, so it is very convenient.

A method of formulating high ambient temperature (room temperature and above) stable biologics (biologically active macromolecules, enzymes, serums, vaccines, viruses, pesticides, drug delivery systems, liposomes, cells suspensions, sperm, erythrocytes, other blood cells, stem cells, multicellular tissues, skin, heart valves) including secondary drying comprising at least two steps of stability drying at elevated temperature: 35° C., 40° C., 45° C., 50° C., and higher temperatures. The method could be applied to stabilize biologics encapsulated in alginate gel microspheres for better oral delivery. The method encompasses the following: microspheres are formulated using a cryo-encapsulation procedure comprised of mixing drops of frozen preservation mixture (To form the preservation mixture, biologics are mixed with preservation solutions containing sodium alginate.) with frozen drops of a calcium solution (i.e. calcium gluconate) and subsequent warming to form the gel particles.

The invention relates to a body anti-corrosive preservation solution. The raw materials in percentage by weight of the preservation solution are: 10 to 40 percent of glycerol, 10 to 70 percent of ethanol, 1 to 20 percent of metacetonic acid, 0.1 to 20 percent of hexamethylene tetramine, 0.2 to 10 percent of 5-chloro-2-methyl-4-isothiazoline-3-ketone, 0.1 to 1 percent of peppermint oil and the balance being water. The application mode of the preservation solution comprises the following steps: the anti-corrosive preservation solution is injected into the digestive system of a body from the oral cavity or the nose and the throat of the body; the anti-corrosive preservation solution is injected through an aortic arch, a brachial artery or a femoral artery; and the anti-corrosive preservation solution can also be used to wipe the surface of a body by a tampon dipped in the preservation solution. The body anti-corrosive preservation solution is nontoxic and harmless to an operator and is easy to operate; moreover, the preservation solution has no influence on the environment and excellent anti-corrosive effects, and can ensure that a body maintains a natural state and softness in a natural state (at room temperature) for 5 to 12 days. In addition, the preservation solution can be used repeatedly and has a simple preparing method, easily obtained raw materials and strong maneuverability; moreover, the preservation solution has convenient practical application and is propitious to popularize and use.

The present invention provides compositions and methods for preservation, perfusion or reperfusion of an organ, such as heart, for transplantation. The compositions comprise a preservation solution and an effective amount of neuregulin. The methods comprising contacting an organ with an effective amount of neuregulin.

The invention discloses a simple thin liquid-based cytology examine method applied for the gynecological examination, the cervical cancer general examine and the cervical cancer preceding lesion of selected crowd, the steps including: using the cervical cells brush and the scraper to collect samples, put samples in the liquid-based cell preservation solution, add base liquid cell processing solution, oscillate, centrifugally separate, take and discard the supernatant samples liquid, using adding samples gun to assimilate sediment samples, smear, sheet drying, using 95% ethanol marinate or spray for fixed, washing and dyeing. The invention has simple method, small steps, high positive detection rate, low misdiagnosis rate, low cost, and it can effectively eliminate the red blood cells, mucus and other interference components from the cervical cytology detection specimens, with good sheet-producing result.

The present invention discloses an RNA virus inactivation preservation solution. The inactivation preservation solution comprises ammonium salt, 1-ethyl-3-methylimidazolium bisulfate, lithium chloride, ethylenediaminetetraacetic acid, an RNase inhibitor, a surfactant, a reducing agent, a buffer agent and water. The present invention also provides an RNA virus sample inactivation and preservation method and an RNA virus detection method, and the method is conducted by using the inactivation preservation solution. The preservation solution has low cost, inhibits RNase activity, preserves RNA integrity, realizes preservation and transportation of samples at room temperature, can also quickly inactivate pathogens at room temperature, reduces infection risks of medical personnel, and reduces false negatives in detection. At the same time, the inactivation preservation solution can realize a lysis function, does not contain chaotropic reagents and alcohols, is compatible with mainstream nucleic acid extraction kits on the market, greatly saves time of an entire process, and is particularly suitable for 2019 new coronavirus (COVID-19) inactivation and lysis steps after swab sampling.

The invention discloses an immunomagnetic beads preservation solution, which comprises a buffer, an inert protein, glycerin, a water-soluble sugar, a synthetic high molecular polymer, a protein stabilizer, a reducing substance, an amino acid, a chelating agent, a serum, a surfactant, and a preservative. According to the immunomagnetic beads preservation solution of the invention, the activity of immunomagnetic beads can be maintained at a high level within one year at 4 DEG C storage conditions, thereby providing a guarantee for the practical detection of the immunomagnetic beads.

The present invention provides a liquid for preserving cornea for medium period and a preparing method thereof, and relates to a liquid for cornea material. The invention provides the liquid for preserving cornea for medium period and the preparing method thereof, wherein the liquid has the advantages of longer storing time, higher quality, no pollution and relatively lower cost compared with theprior liquid for preserving cornea for medium period. The liquid for preserving cornea for medium period contains culture solution, thickening agent, bovine serum, amino acid, antibiotic, acid-alkalimodifying agent and cell nutrition components. According to the formula of liquid for preserving cornea for medium period, the thickening agent, the bovine serum, the amino acid, the antibiotic and cell nutrition components are added into the culture solution for mixing to uniform. The pH value is adjusted to 7.2-7.4 with the acid-alkali modifying agent. The osmotic pressure is adjusted to 350-380mOsm/L with an osmotic pressure buffering agent. The liquid for preserving cornea for medium period is obtained after membrane filtering for sterilizing.

The invention discloses a human feces DNA extraction method. The prior art is complicated and time-consuming; and the trace DNAs extracted by the previous sampling method is too little to perform molecular diagnosis. The extraction method uses selective sample collection PSC equipment for feces samples and adopts a low temperature preservation method. The extraction method comprises the following steps: picking a sample by using a sterilized gun head or sterilized blade and directly placing the picked sample on ice; and adding a feces lysis solution, fully cracking and evenly mixing, adding bovine serum albumin, centrifuging to remove the supernatant, adding protease K to perform a digestion reaction at 58 DEG C, then adding a protein precipitation solution to precipitate the protein, centrifuging to obtain the supernatant, adding a silica gel membrane binding solution to fully bind DNAs, centrifuging to obtain precipitates, adding a silica gel membrane washing solution to wash twice, centrifuging, and adding a TE (Tris + EDTA) buffer solution to finally obtain a preservation solution with DNAs. The human feces DNA extraction method has the advantages of high repeatability and stability, simpleness and short time.

The present invention discloses a saliva preservation solution, a preparation method and uses thereof, wherein the saliva preservation solution comprises 0.1-2 mol/L of lithium chloride, 1-50 mmol/L of Tris-HCl, 0.1-2 mol/L of urea, 0.1-2% (w/v) of SDS, 1-10 mmol/L of EDTA, and 10-90% (v/v) of ethanol, and the pH value of the system is 7-10. The saliva preservation solution of the present invention is used for preserving saliva, can maintain the integrity of DNA in cells for a long time, and can prevent bacteria and other microorganisms from breeding.

The invention discloses a preservation solution for exfoliated cell and a preparation method which uses the preservation solution to process cell thin layer smear. The preservation solution for exfoliated cell comprises the components which are prepared by the volume proportion of 10 to 15 portions of methanol, 10 to 15 portions of alcohol, 1 to 2 portions of glycerin, 1 to 2 portions of glycol polyethylene, 10 to 12 portions of formaldehyde, 0.5 to 1 portions of glacial acetic acid, 10 to 20 portions of sodium chloride with 0.85 percent to 0.9 percent of mass concentration and 33 percent to 68.5 percent of phosphate buffer. The steps of the preparation method for cell thin layer smear are that: 1) the collected sample is put in the preservation solution for exfoliated cell for shaking; the sample is transferred to a centrifuge for centrifugal treatment after standing; 2) the supernatant is removed until 1ml to 2ml residue is left in the centrifuge tube; the cell suspension is obtained after uniform vibration; 3) 0.5ml to 1ml cell suspension is put into a smear preparation cup which is made into cell thin layer smear by a cell smear preparation machine.

The invention relates to an organ preservation solution and a method for preparing the same. The method comprises the following steps of: (1) adopting double buffer pairs of citric acids and phosphates to enable the PH value of a solution to be maintained in a range of 7.5 to 8; (2) adding active ingredients such as adenosine, L-arginine, tryptophan, ligustrazine hydrochloride, reduced glutathione and the like and keep the solution stable; (3) to maintain a hypertonic property of the preservation solution with an osmotic pressure of 350-380 mOsm/L, adopting trehalose and gluconolactone as impermeable substances to effectively prevent cellular edema; and (4) adopting polyethylene glycol with a large molecular weight as a colloidal substance to effectively prevent extracellular space from expanding. Compared with the prior art, the method employs raw materials which are all homemade medicinal components and meet the standards of national pharmacopoeia, and are cheap and free of incompatibility; the organ preservation solution prepared is a colorless and transparent liquid which has stable properties, and can be sterilized by high temperature and high pressure, stored at normal temperature or low temperature, and refrigerated, and thus preparation, transportation, storage and usage of the organ preservation liquid are all very easy and convenient.