67 results about "Preservation solutions" patented technology

The invention discloses a liquid cell product, a preparation method thereof and a preserving fluid for preparing the liquid cell product, and belongs to the technical field of immunodetection. The dendritic polymer is creatively added into the preserving fluid for preparing the liquid cell product, the cell morphology in the liquid cell product can be improved, and a thermal acceleration test proves that the preserving fluid can maintain the cell morphology in the liquid cell product for a long time. When the preservation solution is used for preparing the liquid cell quality control product for immunodetection, quality control spots with complete circles can be formed after sample application on slides made of different materials, it can be obviously judged that cells in the liquid cell quality control product are dispersed very uniformly after immunohistochemical staining, and the preservation solution has very high application value.

The invention relates to a fresh flower preservation method which sequentially comprises the following steps: manually poking grape seeds, cleaning and drying, grinding powder to obtain grape seed powder, adding ethanol in a ratio of 1: 10, adopting a microwave soaking method to obtain a preservation solution, diluting by 50 times, and uniformly spraying on leaves by adopting a spraying method. According to the fresh flower preservation method provided by the invention, the storage time of the petals of the fresh flowers can be prolonged, the colors of the petals cannot be obviously changed after the petals are placed for a long time, meanwhile, the period of water loss and withering is also greatly prolonged, and the sales range of the fresh flowers is expanded.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The utility model discloses a urine sample collection and preservation device, which belongs to the technical field of sample experiment reaction and comprises a preservation container and a collection container, the collection container is sleeved outside the preservation container and is detachably matched with the preservation container, the collection container is provided with an open collection cavity, and the preservation container is provided with a sealed storage cavity; the storage container is of a transparent structure and is provided with at least a first scale mark and a second scale mark, in the storage cavity, a first capacity space is formed between the first scale mark and the bottom of the storage container, and a second capacity space is formed between the second scale mark and the bottom of the storage container. The volume of the second capacity space is larger than that of the first capacity space. The first scale mark and the second scale mark are used as marks, the preserving fluid is quantitatively added, the urine sample and the preserving fluid are quantitatively mixed and preserved, it is ensured that the urine sample is relatively stable, cells in the urine sample are well preserved, and the accuracy of detection data is obviously improved.

The invention provides a preservation method of mold mycelium pellets. According to a preservation solution used for low-temperature preservation, the COD concentration is 500-1000 mg / L, the NH4 < + >-N concentration is 100-300 mg / L, the PO4 < 3->-P concentration is 20-50 mg / L, the metal ion concentration is 0.5 mg / L, and the pH value is 2-3. The preservation solution for normal temperature preservation has the COD concentration of 100-300 mg / L, the NH4 < + >-N concentration of 20-50 mg / L, the PO4 < 3->-P concentration of 5-10 mg / L, the metal ion concentration of 0.2 mg / L and the tea polyphenol concentration of 1-2 g / L, and the pH value is adjusted to 2-3. With the adoption of the preserving fluid, strain cells in the mycelium pellets are kept in a low-energy state, only weak endogenous metabolism is performed, mycelium pellet cells are not split, the mycelium pellets can keep lasting activity, and the storage conditions of the mold mycelium pellets are improved, so that the utilization rate of the mycelium pellets is greatly increased.

The invention relates to the technical field of liquid-based cytology, and particularly discloses serosa effusion cell preserving fluid. Comprising a preservation solution, an anticoagulant, a red blood cell treatment solution, a cell membrane stabilizer, an antioxidant and a metal ion chelating agent, the preservation solution contains a buffer system, an energy supply component, an antibiotic and a surfactant, and the red blood cell treatment solution contains a hemolysis active component and a cell immobilization component; the preparation method comprises the following steps: firstly, preparing the preserving fluid, adjusting the pH value, adding the active substances, mixing the antibiotics, and then completing constant volume and sterile filtration; the anticoagulant solution and the red blood cell treating fluid are independently prepared, and sterilization is achieved through filtration; and finally, packaging all the components in a sterilized storage bottle in a matched manner. The composition provided by the invention can be used for preserving cells in a clinical serosal effusion sample, and can provide a high-quality sample for subsequent cell morphology observation or detection; in addition, according to the preparation method, the controllability is high when all the components are independently prepared, and the preserving fluid with the consistent performance can be stably prepared.

This invention discloses a method for preparing large-scale, original-color seaweed specimens, belonging to the field of seaweed preparation methods. The method includes the following steps: S1: Collecting algal bodies with reproductive organs and basal holdfasts; S2: Washing the algal bodies from S1 with pretreated water, selecting algal bodies with intact reproductive organs and basal holdfasts, and drying them; S3: Completely wrapping the algal bodies from S2 with plastic wrap and irradiating them with ultraviolet light; S4: Preparing a preservation solution; S5: Immersing the algal bodies from S3 in the preservation solution; removing them after 5 minutes; S6: Preparing the original-color specimen: removing the algal bodies from S5, placing them in a paper bag, and air-drying them in a dry, ventilated location. The dried specimen is then the original-color specimen. This method can produce original-color seaweed specimens.

The present application relates to a high concentration of ammonium bisulfite preservation solution, the preservation solution components include ammonium bisulfite and ethanol, the concentration of ammonium bisulfite is about 13 mol / L to about 15 mol / L, the concentration of ethanol is about 0.3 mol / L to about 1.5 mol / L. The present application also provides a method and kit for preserving high concentration of ammonium bisulfite solution.

The application relates to the technical field of medicines, in particular to a lymphocyte preservation solution, a preparation method and application in sample pretreatment, and the lymphocyte preservation solution is prepared from the following components in parts by mass: Tris-HCl buffer 90-100 parts, trehalose 10-15 parts, trichostatin A 0.01-0.03 parts, nuclease inhibitor 0.3-0.5 parts and the like raw materials. Through the synergistic design of a composite buffer system and functionalized nanomaterials, combined with multi-stage active protection technology, cell metabolism regulation and precise isolation of external interference factors are realized. The application improves the processing efficiency of complex samples, prolongs the preservation time of key biomarkers, and enhances the adaptability in extreme environments. The integration of the innovative preparation process and the intelligent quality control module provides reliable support for immune diagnosis and treatment, rare cell research and remote medical treatment, and has wide clinical application and scientific research transformation prospects.

This invention discloses a simple long-term preservation method for small insect specimens used in DNA molecular detection, comprising the following steps: Freshly collected insect specimens are placed in centrifuge tubes filled with anhydrous ethanol, which is added to 4 / 5 of the tube's height, and a label is written on the tube before placement; insects of the same species from the same collection site are classified under a dissecting microscope and placed in the same centrifuge tube; after classification, centrifuge tubes from the same collection site are placed in the same sealed container, and a magnesium sheet wrapped in a water-permeable material is placed in the container, with anhydrous ethanol added until the magnesium sheet is submerged; the sealed container is then stored away from light, and anhydrous ethanol is added periodically. This invention solves the problem of preservation solution evaporation due to poor sealing of centrifuge tubes, and also reduces the water content of the anhydrous ethanol in the outer container, enabling long-term or permanent preservation of insect specimens, and allowing for quick identification based on label markings.

A cell preservation solution composition, excluding dimethyl sulfoxide (DMSO), includes a copolymer of compound A and compound B. In a composition of 100 wt % of the cell preservation solution composition, the compound A ranges from 60.00 wt % to 99.95 wt %, and compound B ranges from 0.05 wt % to 40.00 wt %.

The present application relates to the technical field of biology, and particularly relates to a use of a cell preservation solution in preparation of an immunosuppressant. The present application provides a use of a cell preservation solution in preparation of an immunosuppressant, the cell preservation solution is obtained by a specific method, and the cell preservation solution is applied to a preparation (such as an injection solution) containing mesenchymal stem cells, so that the injection solution is endowed with certain immunosuppressive function, the combination with MSCs cells improves the treatment effect, and the long-time transportation capacity and the survival rate of MSCs are improved.

The invention belongs to the field of biology, and discloses a tissue formula preserving fluid containing antimycin A. The tissue formula preserving fluid comprises a basic culture medium, the basal culture medium contains superoxide dismutase, catalase and antimycin A. On the basis of the superoxide dismutase and the catalase, the preservation solution is matched with the antimycin A, so that the activity of tissue cells can be remarkably improved; in the research process, we have the following specificity that: 1, when the respiratory chain inhibitor drug is applied to the formula of the invention, the improvement degree of the cell viability of only antimycin A is the highest; 2, the function of antimycin A must be exerted based on the use of superoxide dismutase and catalase; meanwhile, the invention further provides a tissue preservation method.

The invention relates to the technical field of storage, in particular to an oral cavity sample storage device which comprises a storage tube, a one-way piece, a collection rod, a sealing column and an interaction plate, and a complete technical scheme is formed through optimization of multiple embodiments. During collection, a sample is obtained through the ball head of the collection rod, and after the storage tube is placed, a preservation solution generates impact and suction through the through hole of the interaction plate by shaking, so that the sample is quickly and uniformly dissolved, and long-term soaking of the collection head is avoided. The one-way piece is designed through a buffer groove, a piston and a push rod, so that the preservation liquid is controllably discharged in a dripping mode, and overflow is prevented. The sealing column is matched with a double-horn-mouth and supporting rod structure through a sliding block-sliding groove-limiting groove, rapid positioning and limiting are achieved, sealing is enhanced, the shaking space is reduced, and the dissolving efficiency is improved. The device avoids detection impurity interference, is reliable in sealing and convenient to operate, adapts to various detection scenes and sample requirements, considers sample storage stability and detection accuracy, and greatly improves the pretreatment efficiency.

The application provides a method for improving the effect of sperm freezing preservation, and particularly relates to adding sperm into a freezing preservation solution containing carnosine to perform freezing preservation. The freezing preservation solution added with carnosine can effectively improve the activity of the sperm after freezing. The addition of carnosine into the sperm freezing preservation solution can effectively improve the quality of the sperm after freezing preservation, and provides certain technical support for sperm preservation.

This invention relates to the field of fecal genomics kit technology, and particularly to a safe, rapid, and high-throughput kit for extracting fecal genomes and its usage method. The kit includes a sample preservation solution, magnetic beads, a lysis buffer, a washing buffer 1, a washing buffer 2, and an elution buffer. The sample preservation solution is composed of guanidine isothiocyanate, sodium citrate, Triton X-100, Tween 20, propidium iodide, and purified water. The magnetic beads are hydroxyl magnetic beads. The lysis buffer is composed of guanidine hydrochloride, Tris base, Tris hydrochloric acid, Triton X-100, isopropanol, and purified water. Washing buffer 1 is composed of guanidine hydrochloride solution and isopropanol. Washing buffer 2 is composed of anhydrous ethanol and purified water. This kit can easily extract the genome from fecal samples, is simple to operate, safe and non-toxic, and can extract multiple samples at once, enabling rapid diagnosis and filling the gap in the current market for automated high-throughput kits for extracting genomes from fecal samples.

This invention discloses a preservation solution for preserving sample DNA at room temperature and its preparation method, belonging to the field of biological sample preservation technology. The preservation solution comprises: a lysis system, a nucleic acid protection system, and a buffering and stabilizing system; the lysis system comprises a complex surfactant and an enzymatic hydrolysis aid; the complex surfactant comprises: polyether polyol fatty acid ester and cocamidopropyl hydroxysulfonate betaine; the enzymatic hydrolysis aid comprises lysozyme Lyso-V and proteinase K; the nucleic acid protection system comprises: nitrogen-heterocyclic polyamine-carboxylic acid derivatives and dextran sulfate; the nitrogen-heterocyclic polyamine-carboxylic acid derivatives comprise: 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''tetraacetic acid, 1,4,7-triazacyclononane-N,N',N''-triacetic acid, disodium ethylenediaminetetraacetate-nitrocyclic derivative, and diethylenetriaminepentaacetic acid-piperazine derivative; the buffering and stabilizing system comprises: an amphoteric buffer and a polymeric stabilizer; the amphoteric buffer comprises: 2-(N-morpholino)ethanesulfonic acid and N-tris(hydroxymethyl)methylglycine.

This invention discloses a method for preserving surgical transplant materials, a combination of transplant materials, and a skull transplant material, belonging to the field of biomedical engineering and surgical transplant material processing technology. The method includes the following steps: skull harvesting and pretreatment, preparation and precooling of preservation solution, pulsed xenon light sterilization, sealing of the surgical transplant material, and cryopreservation. This application employs a pulsed xenon light sterilization method for preserving surgical transplant materials, which solves the problems of chemical sterilization potentially causing residual penetration and toxicity to bone cells, high-temperature sterilization leading to collagen denaturation and cell inactivation, and irradiation sterilization potentially causing DNA damage to cells and incurring high equipment costs. This method maintains the cell activity of the transplant material after long-term cryopreservation while improving the osteogenic induction and healing quality of the transplant material, and also has low preservation costs.

The present application relates to the technical field of biology, and discloses a cell preservation solution, a preparation method and preparation thereof, and a cell preservation method. The cell preservation solution contains 1-10 mL compound electrolyte glucose injection, 1-10 mL human blood albumin injection, 1-10 mL compound amino acid injection, 10-20 uM salvianolic acid B, 20-60 uM rhodioside, and 1-10 uM ginkgo biloba bilobal, and the compound electrolyte injection is supplemented. The cell preservation solution can improve the biological activity of cells and prolong the preservation time of cells in multiple ways by adding traditional Chinese medicine extracts as antioxidants, membrane stabilizers and antithrombotics in response to the excess of free radicals in the low-temperature preservation process.

The invention discloses deep learning-based deep sea cold spring microbiome antibacterial peptide directional screening and application thereof, and belongs to the technical fields of deep sea extreme environment microbial resource high-value utilization, food preservation and fresh-keeping and bioinformatics. The antibacterial peptide DSCSAMP1-4 applicable to the soy sauce in the high-salt environment is directionally screened by constructing a data set containing polypeptide physicochemical properties, structural characteristics and weighted scores. Wherein the DSCSAMP2-3 containing the alpha-helical structure has obvious growth inhibition on gram-negative bacteria such as escherichia coli and pseudomonas aeruginosa, gram-positive bacteria such as staphylococcus aureus and fungi such as saccharomycetes respectively. The antibacterial peptide is natural, safe, wide in antibacterial spectrum, high in acting efficiency and free of chemical residues, does not affect the flavor of soy sauce, can be prepared into preservative additives of various dosage forms, provides an efficient and environment-friendly preservative solution for the soy sauce industry, promotes high-value utilization of deep sea cold spring microbial resources, and has great industrial popularization value.

The present invention relates to a preservation solution for a reverse osmosis membrane element. Based on the total mass of the preservation solution, the preservation solution comprises the following components: 5,900-18,000 ppm of sodium ions, 9,000-30,000 ppm of chloride ions, 1-3,000 ppm of multivalent cations, 2-200 ppm of an ether polymer, 1-20,000 ppm of a non-oxidizing bactericide and a solvent, wherein the solvent comprises water; the multivalent cations comprise divalent and / or trivalent metal ions; and the ether polymer comprises a structural unit derived from ethylene oxide. The preservation solution for a reverse osmosis membrane element in one embodiment of the present invention can be used for preservation of reverse osmosis membrane elements. By using the preservation solution to preserve seawater desalination reverse osmosis membrane elements, the elements can maintain stable water production, salt rejection rate, boron rejection rate and the like even after long-term storage, and the reproduction of microorganisms can be effectively controlled.

The invention belongs to the technical field of microorganism preservation, and particularly relates to normal-temperature preservation liquid for group B streptococcus (GBS) in a vaginal swab as well as a preparation method and application of the normal-temperature preservation liquid. The invention provides a GBS normal-temperature preservation solution. The formula of the GBS normal-temperature preservation solution comprises glycerol, glucose, sodium citrate, EDTA, FBS, compound amino acid, NaCl and the like. The preservation solution ensures that GBS can be stably preserved for 30 days at normal temperature, the activity of GBS and the stability of RNA are effectively maintained, the problems of RNA degradation and bacterial activity loss in an existing preservation method are solved, and reliable sample support is provided for subsequent bacterial culture and RNA detection. The method is suitable for long-time preservation and subsequent detection of clinical vaginal swab samples, and has a good application prospect.

The invention discloses edible fresh-keeping gel beads for a vegetable protein beverage and a preparation method of the edible fresh-keeping gel beads, and belongs to the technical field of functional food and intelligent packaging. According to the edible fresh-keeping gel beads prepared by the preparation method disclosed by the invention, Schiff base formed by perillaldehyde-yeast protein is used as a liquid core, and Ca < 2 + > is used for pre-crosslinking; the wall material is a sodium alginate-rice protein composite network and can intelligently respond to imine bond protonation caused by pH drop in the beverage storage process, so that controllable release of perillaldehyde is realized, protein aggregation and microbial growth are inhibited, and a clean label and original sense are kept. According to the invention, triple advantages of edible, intelligent response and low cost are taken into consideration, and a plug-and-play fresh-keeping solution is provided for the plant-based protein beverage.

The present invention provides a sample collection container that effectively prevents leakage of the contents contained within the container body. [Solution] A sample collection container comprising a lid having a lid portion for sealing the container and a sample collection rod, a container body having a storage portion for storing a sample preservation solution and an opening for inserting the sample collection rod into the storage portion, wherein a pin-stopping portion having a reentrant structure is provided continuously or discontinuously along the circumferential direction of the inner surface of the container body.

This invention discloses a method for cryopreservation of donkey semen using antioxidants, belonging to the field of animal reproductive technology. The method is based on a specific basal diluent, with the addition of targeted mitochondrial antioxidants SKQ1 or SS-31. First, a basal diluent containing sugars, citrate, proline, skim milk powder, and antibiotics is prepared. Then, SKQ1 (dissolved in DMSO) or SS-31 (dissolved in physiological saline) is separately prepared into a stock solution, which is then mixed with the basal diluent to obtain a composite preservation solution. Finally, the donkey semen treated with this composite preservation solution is diluted and stored at 4°C. The method of this invention can significantly improve sperm motility and acrosome integrity rate of donkey semen stored at 4°C for 5 days, reduce ROS content and abnormality rate, and significantly increase the insemination and conception rate of the preserved semen to over 40%, effectively extending the practical preservation period of donkey semen.

This invention provides an immersion preservation method and preservation solution that maintain organs for transplantation in good condition for a longer period than conventional methods. [Solution] The present invention relates to a method for preserving organs for transplantation, which involves immersing the organs in a nitrogen-rich UW solution at a temperature suitable for immersion preservation, where the dissolved oxygen content is 1.0 mg / L or less and the nitrogen content is 90 mg / L or more at one atmosphere. As an example, a nitrogen-rich UW solution is prepared in which the dissolved oxygen content is 1.0 mg / L or less and the nitrogen content is 90 mg / L or more at one atmosphere and a temperature suitable for immersion preservation, and the organs for transplantation are immersed in the prepared nitrogen-rich UW solution. As another example, a nitrogen-rich UW solution is prepared in which the dissolved oxygen content is 1.0 mg / L or less and the nitrogen content is 90 mg / L or more at one atmosphere and room temperature, and the prepared nitrogen-rich UW solution is cooled to a temperature suitable for immersion preservation before the organs for transplantation are immersed in it.

This invention discloses a method for preserving supercooled ex vivo organs. The method involves placing the ex vivo organ in a preservation solution and then cooling it for preservation. The key feature is that the ex vivo organ is placed within a magnetic field range and its temperature is lowered to a supercooled range of 0°C to -5°C for preservation. This invention can better reduce the organ preservation temperature, thereby extending the preservation time and improving the preservation effect and organ viability. It is particularly suitable for clinical organ preservation and research sample preservation.

This invention relates to a fecal preservation solution, its method of use, and its applications, belonging to the field of molecular biology and biosample preservation technology. To address the shortcomings of existing fecal sample preservation methods, which struggle to simultaneously maintain the stability of low-abundance host DNA and inhibit microbial community drift at room temperature, and which suffer from equipment dependence, cumbersome operation, or safety hazards, this invention provides a fecal preservation solution composed of ethanol, inorganic salts, trehalose, and water. It utilizes the fixation effect of ethanol, the high-salt, hypertonic environment, and the molecular protection mechanism of trehalose to produce a synergistic effect. The fecal preservation solution provided by this invention has a very simple composition and low cost, containing only three effective components and no chelating agents or guanidine salts; it is safe and non-toxic, with an ethanol concentration ≤60%, is not a hazardous material, and is easy to operate; it provides dual protection for both microorganisms and host DNA; it is suitable for human and wildlife fecal samples, and the preserved DNA can be directly used for downstream molecular detection without additional purification.

This invention discloses an in-situ vitrification-semi-solid nucleic acid room-temperature stable preservation solution, its preservation method, and a reagent kit, belonging to the field of biological sample preservation technology. The preservation solution comprises a double buffer system, a mild chelation system, a vitrification framework, a thermoresponsive gelling system, an oxidative protection system, a low-toxicity antibacterial system, and water. The thermoresponsive gelling system forms a weakly reversible gel network within the range of 18-37°C after the sample is mixed with the preservation solution, placing the sample nucleic acid in a confined microenvironment with low diffusion and low water activity. This invention achieves long-term stable preservation of nucleic acid samples at room temperature through the synergistic effect of a triple mechanism of "low water activity - thermoresponsive gelling - multi-target passivation." This invention eliminates the need for cold chain transportation, is simple to operate, and has high safety, making it suitable for the room-temperature collection, transportation, and preservation of various biological samples such as blood, saliva, oral swabs, sputum, and tissue homogenates.

The utility model provides a medical clinical microbiological detection sampling device and relates to the technical field of medical sampling. The medical clinical microbiological detection sampling device comprises a storage box, a heating wire is arranged in the storage box, a placement groove is formed in the storage box, a medicine bottle is arranged in the placement groove, a sealing cover is arranged at the upper end of the medicine bottle, a rubber cover is arranged in the sealing cover, and a sample conveying pipe is connected into the rubber cover. The side end of the sample conveying pipeline is connected with an air bag. According to the medical clinical microbiological detection sampling device, the medicine bottle and the heating wire are arranged, a specific microbiological preservation solution is injected into the medicine bottle, the heating wire is electrified to generate heat, heat is supplied to the medicine bottle, the human body temperature is simulated, and the temperature condition close to the original living environment of microorganisms can be provided for the microorganisms; the activity of the microorganisms can be maintained in the period from the time when the microorganisms are taken out of the human body to the time before detection, so that the detection result can more accurately reflect the real condition of the microorganisms in the human body.