198 results about "Preservation solutions" patented technology

The invention relates to the technical field of biology, in particular to a sample preservation solution for vagina micro-ecology detection and a preparation method of the sample preservation solution. The invention relates to a sample preservation solution for vaginal micro-ecology detection. The sample preservation solution comprises the following components in percentage by mass: 0.5-0.9% of paraformaldehyde, 0.08-0.12% of glutaraldehyde, 10-18% of a buffer solution, 0.1-0.4% of inositol, 1-10% of a stabilizer, 0.8-5% of a cell treatment agent and the balance of water, the stabilizing agent is prepared from sodium alginate, tween-20 and beta-cyclodextrin. The invention aims to solve the problems of high false negative and false positive rate or difficulty in interpretation caused by liquid-based cell accumulation, unstable subsequent dyeing and decoloration time and the like of a cell preservation solution in the prior art, so that the sample preservation solution for sample cells can keep the forms of cells, protozoa and the like unchanged, and ensures that gram staining negative and positive properties of bacteria and fungi are not changed. The staining and decoloring are relatively stable, the false negative and false positive rate of sample cell detection is effectively reduced, and the sample cell lesion detection and judgment are more accurate.

The invention belongs to the field of biological medicine, and provides a protection liquid capable of preserving in-vitro animal organs or in-vitro animal tissues at normal temperature as well as a preparation method and application of the protection liquid, and the protection liquid comprises a solution containing a hemoglobin oxygen carrier or a hemoglobin carrier, an osmotic pressure regulating substance and the like. The protective liquid provided by the invention can be used for preserving or perfusing broken limbs, isolated animal organs or isolated animal tissues at normal temperature, and can also be used as a culture liquid of artificial organs and artificial tissues.

The invention discloses a liquid cell product, a preparation method thereof and a preserving fluid for preparing the liquid cell product, and belongs to the technical field of immunodetection. The dendritic polymer is creatively added into the preserving fluid for preparing the liquid cell product, the cell morphology in the liquid cell product can be improved, and a thermal acceleration test proves that the preserving fluid can maintain the cell morphology in the liquid cell product for a long time. When the preservation solution is used for preparing the liquid cell quality control product for immunodetection, quality control spots with complete circles can be formed after sample application on slides made of different materials, it can be obviously judged that cells in the liquid cell quality control product are dispersed very uniformly after immunohistochemical staining, and the preservation solution has very high application value.

The invention provides a low-temperature-resistant inactivated guanidine salt virus preservation solution and a preparation method thereof, and relates to the field of virus preservation solutions. Comprising the following raw materials: NaCl, KCl, glucose, KH2PO4, Na2HPO4, guanidine hydrochloride, penicillin, streptomycin, amphotericin B and glycerol. The raw materials are mixed and uniformly stirred. The preserving fluid is suitable for being used in a low-temperature environment, can preserve a liquid state within several hours in the low-temperature environment, can significantly inactivate pathogens, can also maintain completeness of subsequent nucleic acid extraction, and has no significant influence on amplification and the like.

The invention relates to the technical field of organ transplantation, and discloses organ preserving fluid, application and organ dynamic supercooling perfusion equipment. The organ preserving fluid is prepared from ribose, glucose, mannitol, lidocaine, adenosine, a high-molecular polymer, L-proline, trehalose, inorganic salt and organic salt. The high-molecular polymer comprises one of hydroxyethyl starch, polyethylene glycol and albumin. According to the organ preserving fluid disclosed by the invention, the freezing point of the perfusate is reduced through the micromolecular cryoprotectant, and meanwhile, the micromolecular cryoprotectant and the high-molecular polymer jointly inhibit formation and growth of ice crystals; the problem of liquid preservation at the temperature below zero is solved from the two dimensions of physics (reducing the freezing point and inhibiting ice crystals) and biology (maintaining cell viability), and feasibility is provided for long-time low-temperature transfer and transplantation of organs. The preserving fluid can be used for static preservation or perfusion preservation of various organs such as kidney, liver, heart, pancreas, lung, small intestine, skin and the like.

The present invention relates to a sputum sample microbial flora and nucleic acid DNA preservation solution, including the following components by mass: 0.5-2 parts of trishydroxymethylaminomethane, 0.2-1 parts of mucus digestive agent, 3-6 parts of N-octylpyridinium bromide, 20-50 parts of fixative, 0.6-1.5 parts of auxiliary fixative, 1-2 parts of metal ion chelating agent, 0.5-3 parts of inorganic salt, the metal ion chelating agent includes at least 50wt% of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid; the auxiliary fixative is selected from at least one of sodium citrate and potassium citrate. The sputum sample microbial flora and nucleic acid DNA preservation solution using the technical solution of the present invention has the following advantages, can be stored and transported at room temperature for a long time, and is easy to operate; the sputum sample can be effectively liquefied by immersing the sputum sample in the protective solution, and the structural composition of the microorganism is stabilized at room temperature, protecting the diversity of its flora structure and DNA stability.

The invention belongs to the technical field of fruit preservation, and particularly relates to a litchi preservation solution and application thereof, the litchi preservation solution comprises the following components by weight: 0.5-2% of chitosan, 0.05-0.3% of plant essential oil, 0.05-0.2% of L-cysteine, 0.05-0.2% of gamma-polyglutamic acid, 0.5-3% of citric acid, 0.5-2% of calcium chloride, 0.5-4% of a surfactant, 1-5% of ethylene glycol, and the balance of water. The plant essential oil is composed of lemon essential oil, ginger essential oil and clove essential oil, and the mass ratio of the lemon essential oil to the ginger essential oil to the clove essential oil is (1-4): (1-4): (1-4). The litchi fresh-keeping liquid prepared by the invention is safe and non-toxic, and has an excellent fresh-keeping effect on harvested litchis.

The invention discloses a red blood cell room-temperature preservation solution as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The erythrocyte preservative comprises the following components in parts by weight: 1-200 parts of an anticoagulant, 1-200 parts of a blood cell stabilizer, 1-100 parts of a metabolic inhibitor, 1-100 parts of an electrolyte, 1-150 parts of an antioxidant and 350-1000 parts of deionized water, and the pH value of the erythrocyte preservative is 4.6-7.0. All the components in the preservative are mutually matched and have a synergistic effect, and the integrity of erythrocyte membranes can be kept through synergistic protection of the multiple components; the electrolyte equilibrium osmotic pressure is maintained, the antioxidant scavenges free radicals, and hemolysis is reduced; the preservation time is prolonged, and the cell morphology and function are good; the dependence on a cold chain is reduced, and the demand on deep hypothermia equipment is reduced.

The invention relates to a fresh flower preservation method which sequentially comprises the following steps: manually poking grape seeds, cleaning and drying, grinding powder to obtain grape seed powder, adding ethanol in a ratio of 1: 10, adopting a microwave soaking method to obtain a preservation solution, diluting by 50 times, and uniformly spraying on leaves by adopting a spraying method. According to the fresh flower preservation method provided by the invention, the storage time of the petals of the fresh flowers can be prolonged, the colors of the petals cannot be obviously changed after the petals are placed for a long time, meanwhile, the period of water loss and withering is also greatly prolonged, and the sales range of the fresh flowers is expanded.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention discloses a preserving fluid for preserving RNA (Ribonucleic Acid) nucleic acid in a biological sample at normal temperature, and belongs to the technical field of biological preserving reagents. The preservation solution comprises the following components in percentage by mass volume: 10-30% of dimethylacetamide, 90-200mM of inorganic salt, 1-10M of cracking agent, 20-100mM of chelating agent, 0.5-5% of reducing agent, 1-5M of stabilizing agent and 30-200mM of buffer salt, wherein the pH (Potential of Hydrogen) of the preservation solution is 6.0-9.0. According to the invention, dimethylacetamide is used as a preservative, so that a biological sample can be preserved at normal temperature for 15 days without degrading RNA nucleic acid. The preserving fluid provided by the invention is very suitable for preserving RNA nucleic acids, especially miRNA, can provide important guarantee for medical detection based on miRNA, and has very important clinical application value.

The invention discloses ultralow-temperature preservation liquid for Sinilabeo decorus tungting sperms as well as a preparation method, a preservation method and application of the ultralow-temperature preservation liquid and belongs to the technical field of artificial breeding of Sinilabeo decorus tungting. The ultralow-temperature preservation liquid comprises a freezing diluent, an antifreeze agent glycol and an antioxidant, the antifreeze agent accounts for 8% of the volume of the ultralow-temperature preservation liquid, the antioxidant comprises reduced glutathione and alpha-lipoic acid, and the balance is the freezing diluent. According to the present invention, the formula compounding is different from the simple superposition of the traditional formula, the ratio and the combination mode of the components are precisely optimized, the combination has the synergistic effect, the resuscitation sperm activity is improved, the good oxidation resistance is provided, and the effect far better than the effect of other schemes is achieved. The method has remarkable advantages in efficient preservation of the sinilabeo decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus

The invention relates to the technical field of reagent preservation, in particular to biotinylated tyrosine phosphatase antigen preservation liquid as well as a preparation method and application thereof. The pH value of the biotinylated tyrosine phosphatase antigen preserving fluid is 7.0-7.5, and the biotinylated tyrosine phosphatase antigen preserving fluid is prepared from the following raw material components: a buffering agent, soluble metal salt, an organic dispersing agent, poly (ethylene glycol)-block-poly (propylene glycol)-block-poly (ethylene glycol) 8400, a metal ion complexing agent, bovine serum albumin, a protein protective agent and a preservative. By optimizing the components of the biotinylated tyrosine phosphatase antigen preserving fluid, when the prepared antigen preserving fluid is used for preserving a biotin-labeled tyrosine phosphatase antigen, the storage life is greatly prolonged under the condition of 2-8 DEG C. The biotinylated tyrosine phosphatase antigen preserving fluid prepared by the method is convenient to use, stable to store, economical and practical, and simple to prepare.

The invention discloses a composition and a method for constructing ovarian organoid based on a clinical tissue sample. The composition comprises a sample preservation solution, a sample digestive juice and a sample culture medium, the method comprises the following operation steps: transporting a preservation solution A to a laboratory, and pretreating to obtain tissue fragment precipitates; carrying out resuspension incubation on the tissue fragment precipitate by using digestive juice B for digestion; collecting tissue blocks which are not completely digested and filtered, and performing secondary digestion to obtain single cells; combining the cells obtained in two times to obtain the required single cell; adding matrigel, then adding the mixture into a pore plate, and putting the pore plate into an incubator until the matrigel is solidified; and taking out the pore plate, adding a culture medium L2, and culturing to obtain the ovarian organ constructed based on the clinical sample. According to the method, the ovarian organoid is efficiently constructed from the ovarian tissue sample; the provided culture solution formula makes up the dilemma that normal ovarian tissue organoids are difficult to form and normal tissue-derived organoids are difficult to passage at present, and reduces the burden and experiment cost brought by sampling in the disease research process.

The invention belongs to the field of chemiluminescence, and particularly relates to a preservation solution of an acridinium ester marker, a kit and a preparation method and application of the preservation solution. According to the preservation solution of the acridinium ester marker, the sodium caseinate and the BSA are adopted as protein stabilizers, and the specific surfactant, the preservative and the neutral salt range are matched to serve as the preservation solution, so that the stability of the acridinium ester and the marked object of the acridinium ester can be kept. In addition, when the preservation solution acridinium ester marker is adopted for dilution, the diluted reagent can be placed for at least 8 days at 37 DEG C, background signals can be effectively detected, and the detection sensitivity can be improved.

The utility model discloses a urine sample collection and preservation device, which belongs to the technical field of sample experiment reaction and comprises a preservation container and a collection container, the collection container is sleeved outside the preservation container and is detachably matched with the preservation container, the collection container is provided with an open collection cavity, and the preservation container is provided with a sealed storage cavity; the storage container is of a transparent structure and is provided with at least a first scale mark and a second scale mark, in the storage cavity, a first capacity space is formed between the first scale mark and the bottom of the storage container, and a second capacity space is formed between the second scale mark and the bottom of the storage container. The volume of the second capacity space is larger than that of the first capacity space. The first scale mark and the second scale mark are used as marks, the preserving fluid is quantitatively added, the urine sample and the preserving fluid are quantitatively mixed and preserved, it is ensured that the urine sample is relatively stable, cells in the urine sample are well preserved, and the accuracy of detection data is obviously improved.

A processor is configured to: acquire protein information related to a protein contained in a biopharmaceutical and including site information related to a plurality of sites constituting the protein, prescription information related to a prescription of a candidate preservation solution for the biopharmaceutical, and measurement data indicating preservation stability of the candidate preservation solution; derive, based on the protein information and the prescription information, a first feature amount related to the preservation stability of the candidate preservation solution for an entire protein as a target and a second feature amount related to the preservation stability of the candidate preservation solution for each of the plurality of sites as a target; and input the measurement data, the first feature amount, and the second feature amount to a machine learning model and output a score indicating the preservation stability of the candidate preservation solution from the machine learning model.

The invention provides a cleaning and preserving fluid for fetal brain tissues and a preserving method thereof, and belongs to the technical field of tissue preservation. The cleaning and preserving fluid comprises trehalose, nerve growth factors, a GlutaMAX supplement, Y27632, S-nitrosoglutathione, tert-butylhydroquinone, a Versene solution and polyvinylpyrrolidone, compared with a traditional tissue preserving fluid, the cleaning and preserving fluid not only can improve the purity of neural stem cells, but also can maintain the activity and the yield of the neural stem cells in fetal brain tissue, and the cleaning and preserving fluid has the advantages that the cleaning and preserving fluid is simple in structure and convenient to use. The structural integrity of the cells is guaranteed, a high-quality sample is provided for subsequent research on proliferation, differentiation and the like of the neural stem cells, and the method has a wide application prospect.

A pharmaceutical support device includes a processor, in which the processor is configured to: acquire prescription information related to a prescription of a candidate preservation solution that is a candidate for a preservation solution for a biopharmaceutical containing a protein; acquire a plurality of types of measurement data actually measured in a test for confirming preservation stability of the candidate preservation solution; use a machine learning model that outputs prediction data at a future time point of first measurement data, which is at least one type of the plurality of types of measurement data; and input the prescription information, the first measurement data, and at least one type of second measurement data other than the first measurement data among the plurality of types of measurement data to the machine learning model and output the prediction data from the machine learning model.

The invention discloses a microorganism preservation solution, a preparation method, a microorganism sampling device and a use method, and belongs to the technical field of microorganism sampling and preservation. The microorganism preservation solution comprises the following components in parts by volume: 70-75 parts of a buffer solution with the pH value of 7.2-7.4, 5-10 parts of a protective agent, 0.1-0.65 part of an additive, 14-14.5 parts of a nutrient medium and 1-2 parts of an osmotic pressure regulator. The microorganism preserving fluid can ensure that microorganisms keep high activity after sampling, can effectively inhibit excessive proliferation of the microorganisms, avoids sample concentration distortion caused by too fast proliferation or inactivation, and provides reliable guarantee for accurate measurement of target microorganisms. The device comprises: a preservation container, which is provided with an opening and is filled with a microorganism preservation solution; the sampling cover detachably covers the opening in a sealing mode, a scraper and a brush head are installed on the bottom face of the sampling cover, bristles of the brush head are arranged around the scraper in the circumferential direction, and the brush head is located in the storage container and soaked in the microorganism storage liquid. And the sampling cross contamination or exposure risk is reduced.

A drug development assistance device comprising a processor, wherein the processor predicts a stable range in which the stability of a large molecule drug is equal to or higher than a set level for a first condition which is one among a plurality of conditions related to the composition of a preservation solution for the large molecule drug.

The invention provides a preservation method of mold mycelium pellets. According to a preservation solution used for low-temperature preservation, the COD concentration is 500-1000 mg / L, the NH4 < + >-N concentration is 100-300 mg / L, the PO4 < 3->-P concentration is 20-50 mg / L, the metal ion concentration is 0.5 mg / L, and the pH value is 2-3. The preservation solution for normal temperature preservation has the COD concentration of 100-300 mg / L, the NH4 < + >-N concentration of 20-50 mg / L, the PO4 < 3->-P concentration of 5-10 mg / L, the metal ion concentration of 0.2 mg / L and the tea polyphenol concentration of 1-2 g / L, and the pH value is adjusted to 2-3. With the adoption of the preserving fluid, strain cells in the mycelium pellets are kept in a low-energy state, only weak endogenous metabolism is performed, mycelium pellet cells are not split, the mycelium pellets can keep lasting activity, and the storage conditions of the mold mycelium pellets are improved, so that the utilization rate of the mycelium pellets is greatly increased.

The invention relates to the technical field of liquid-based cytology, and particularly discloses serosa effusion cell preserving fluid. Comprising a preservation solution, an anticoagulant, a red blood cell treatment solution, a cell membrane stabilizer, an antioxidant and a metal ion chelating agent, the preservation solution contains a buffer system, an energy supply component, an antibiotic and a surfactant, and the red blood cell treatment solution contains a hemolysis active component and a cell immobilization component; the preparation method comprises the following steps: firstly, preparing the preserving fluid, adjusting the pH value, adding the active substances, mixing the antibiotics, and then completing constant volume and sterile filtration; the anticoagulant solution and the red blood cell treating fluid are independently prepared, and sterilization is achieved through filtration; and finally, packaging all the components in a sterilized storage bottle in a matched manner. The composition provided by the invention can be used for preserving cells in a clinical serosal effusion sample, and can provide a high-quality sample for subsequent cell morphology observation or detection; in addition, according to the preparation method, the controllability is high when all the components are independently prepared, and the preserving fluid with the consistent performance can be stably prepared.

This invention discloses a method for preparing large-scale, original-color seaweed specimens, belonging to the field of seaweed preparation methods. The method includes the following steps: S1: Collecting algal bodies with reproductive organs and basal holdfasts; S2: Washing the algal bodies from S1 with pretreated water, selecting algal bodies with intact reproductive organs and basal holdfasts, and drying them; S3: Completely wrapping the algal bodies from S2 with plastic wrap and irradiating them with ultraviolet light; S4: Preparing a preservation solution; S5: Immersing the algal bodies from S3 in the preservation solution; removing them after 5 minutes; S6: Preparing the original-color specimen: removing the algal bodies from S5, placing them in a paper bag, and air-drying them in a dry, ventilated location. The dried specimen is then the original-color specimen. This method can produce original-color seaweed specimens.

The present application relates to a high concentration of ammonium bisulfite preservation solution, the preservation solution components include ammonium bisulfite and ethanol, the concentration of ammonium bisulfite is about 13 mol / L to about 15 mol / L, the concentration of ethanol is about 0.3 mol / L to about 1.5 mol / L. The present application also provides a method and kit for preserving high concentration of ammonium bisulfite solution.

The invention provides a formula ratio of a cell low-temperature transfer preserving fluid. The cell low-temperature transfer preserving fluid comprises the following components: 100g of sterilized water for injection, 5g of glucose, 5g of human serum albumin (protein), 0.85 g of sodium chloride, 0.03 g of potassium chloride and 0.028 g of calcium chloride. The invention discloses a use method of the cell low-temperature transfer preservation solution, which comprises the following steps: uniformly mixing cells with the cell low-temperature transfer preservation solution to obtain a cell suspension, transferring the cell suspension into a sterile blood transfer bag, and then putting the sterile blood transfer bag into a vaccine box which is provided with an ice box and is precooled in advance for preservation.

The present invention discloses a cell cold storage protection solution and a preparation method and application thereof, and belongs to the field of cell preservation technology. The present invention provides a cell cold storage protection solution, the formula composition includes a compound electrolyte solution, red blood cell preservation solution, heparin sodium, human serum albumin, human platelet lysate and glucose. When preparing the cell cold storage protection solution, mix and prepare it under sterile conditions at room temperature. When the cell cold storage protection solution of the present invention is used to refrigerate a stem cell preparation, the activity of the stem cells is still above 85% after refrigeration for 96 hours, and the growth, proliferation and differentiation potential of the stem cells can be maintained.

The invention belongs to the technical field of preservatives, and particularly relates to a fresh-keeping solution containing walnut green seedcase polyphenol and application thereof in grape preservation, and the fresh-keeping solution is prepared by the following steps: S1, taking dry walnut green seedcases, crushing the walnut green seedcases into powder, putting the powder into a conical flask with a plug, mixing the powder with ethanol, extracting, filtering, collecting filtrate, and concentrating; s2, taking a nano-silver particle solution, adding the nano-silver particle solution into the walnut green seedcase polyphenol extract, and stirring in a stirrer; and S3, adding the modified cyclodextrin into the polyphenol-nano-silver complex solution, stirring, putting into an ultrasonic generator for ultrasonic treatment, centrifuging by using a centrifugal machine, and taking supernate. The polyphenol molecules and the nano-silver particles are included in the cavities of the modified cyclodextrin to form a stable ternary complex, so that the stability of the polyphenol and the nano-silver is improved, and the preservation effect is further enhanced through the antibacterial and antioxidant properties of the chitosan.

The invention relates to the technical field of microorganism preservation, in particular to a novel gonococcus preservation solution which comprises the following components in parts by mass: 1-2 parts of a preservation agent, 40-60 parts of a solvent and 15-25 parts of a protective agent. According to the novel gonococcal preservation solution provided by the invention, the 30% glyceropancreatin soybean broth has a better gonococcal preservation effect than the conventional 10% skim milk at-20 DEG C, so that the gonococcal preservation of outpatient service and basic medical institutions with limited laboratory conditions is facilitated.

The application relates to the technical field of medicines, in particular to a lymphocyte preservation solution, a preparation method and application in sample pretreatment, and the lymphocyte preservation solution is prepared from the following components in parts by mass: Tris-HCl buffer 90-100 parts, trehalose 10-15 parts, trichostatin A 0.01-0.03 parts, nuclease inhibitor 0.3-0.5 parts and the like raw materials. Through the synergistic design of a composite buffer system and functionalized nanomaterials, combined with multi-stage active protection technology, cell metabolism regulation and precise isolation of external interference factors are realized. The application improves the processing efficiency of complex samples, prolongs the preservation time of key biomarkers, and enhances the adaptability in extreme environments. The integration of the innovative preparation process and the intelligent quality control module provides reliable support for immune diagnosis and treatment, rare cell research and remote medical treatment, and has wide clinical application and scientific research transformation prospects.