The invention relates to an antimicrobial moisturizing degradable porous gel preservative film and a preparation method and application thereof. The gel preservative film is formed by polymerizing a carboxyl or amido contained functional monomer, an inorganic nano-powder, biodegradable natural polymer, calcium-ion-contained salt, an inorganic antimicrobial agent and a reductant-oxidant in a water solution. A gel three-dimensional network porous structure is used for spontaneously adjusting gas components inside the film, and the natural polymer is adopted, so that the antimicrobial moisturizing degradable porous gel preservative film has the advantages of being wide in source, biologically degradable, good in film forming property and the like; due to the high antimicrobial property of the inorganic antimicrobial agent, the defects that a traditional preservative film is high in pollution, short in storage period, high in cost, hard to recycle, complex in technology and the like are avoided, and the antimicrobial moisturizing degradable porous gel preservative film can partially replace cardboards, water absorption sponges, ice blocks or plastics and other preservative materials. The preparation method is easy and convenient to implement, capable of promoting large-scale production, long in preservation time, good in permeability, and capable of effectively slowing down the food rotting speed, thereby being applied to food, fruit, vegetable transportation, storage, delivery, assembling, disassembling, storage and preservation in the edible process.