A culture medium unsealing and transferring device relates to the field of container unsealing and comprises a moving plate, a supporting plate, a rotating plate, a rotating shaft and a rotating shaft, the unsealing mechanism is arranged on the supporting plate and comprises a
moving frame moving in the length direction of the unsealing mechanism, a vertically-moving pressing plate is arranged on the inner side of the
moving frame, a plurality of clamping rings are arranged on the pressing plate, a vertically-moving supporting frame is arranged above the pressing plate, a plurality of pressing blocks are arranged on the supporting frame,
mucilage glue is arranged at the bottoms of the pressing blocks, and a plurality of hooks are arranged on the
peripheral sides of the pressing blocks; the clamping mechanism is arranged at one end of the supporting plate and comprises a bearing frame which moves vertically and rotates around one side of the bearing frame, and a plurality of clamping rods are arranged on the bearing frame; the
transfer mechanism is arranged at the other end of the supporting plate and comprises a rotating frame which is movably arranged in the length direction and the vertical direction of the rotating frame, a plurality of clamping blocks are arranged on the rotating frame in the length direction of the rotating frame and used for clamping the conical flasks, and the rotating frame rotates along the connecting line of the centers of the clamping blocks. According to the device, the conical flasks can be unsealed in batches, culture media in the conical flasks can be transferred, efficiency is improved, and the requirement for large-
batch operation is met.