The invention relates to the technical field of
cell culture, and discloses a
cell culture method based on a car-t therapy, which comprises a channel conversion valve, a
peristaltic pump, a dynamic culture unit, a static culture unit, a centrifugal transduction mechanism, an electric
pinch valve, a material bag, a flow meter, a magnetic controller, a
magnetic bead cleaning unit and a waste liquid collection unit. According to the
cell culture method based on the car-t therapy, the
cell culture efficiency is improved, the clinical transformation period is shortened, the
time loss is reduced through automatic material transmission, the
culture environment is accurately controlled, the
cell activity is optimized, the
gene transduction efficiency is improved through a centrifugal transduction mechanism, exogenous
pollution is avoided through a totally-closed process, the
process safety is enhanced, and the
pollution risk is reduced; the whole process is automatic, personal errors are reduced, the process management and control capability is improved in
traceability,
automation and intelligence are achieved, labor dependence is reduced, cultivation cost is reduced, technology popularization is promoted, materials are efficiently utilized, waste is reduced, and labor cost is reduced through
automation.