257results about How to "Increase enzyme activity" patented technology

The invention relates to bacillus velezensis and application thereof in preparation of a water-soluble microbial agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the effects of producing exopolysaccharides, promoting the formation of soil aggregates, degrading abamectin, hydrolyzing protein, producing indoleacetic acid, promoting the growth of tomato plants and promoting the enzyme activity of tomato rhizosphere soil enzyme. The fertilizer not only has the effects of improving the soil aggregate structure and promoting the growth of tomato plants, but also can accelerate the temperature rise of the abamectin fermentation waste residue compost, prolong the high-temperature period and shorten the compost period, and is beneficial to promoting the harmless treatment and resource utilization of the abamectin fermentation waste residue.

The invention discloses a compound enzyme preparation for improving the absorption and utilization rate of a fertilizer and a preparation method thereof, and belongs to the technical field of enzyme preparations. The compound enzyme preparation is prepared from the following components in parts by weight: 100 parts of purified water, 6 to 8 parts of modified cellulase, 10 to 12 parts of immobilized protease, 10 to 12 parts of phosphatase, 10 to 14 parts of urease, 8 to 10 parts of nitrate reductase, 10 to 12 parts of pectinase, 8 to 10 parts of chitinase, 6 to 8 parts of xylanase, 10 to 12 parts of beta-glucosidase, 10 to 12 parts of superoxide dismutase and 4.5 to 6 parts of a microbial agent, the modified cellulase is obtained by chemically modifying cellulase; the immobilized protease is prepared by the following steps: adsorbing activated and coupled modified polylactic acid and a protease solution, crosslinking with glutaraldehyde, filtering, washing and drying. The compound enzyme preparation improves the fertilizer absorption and utilization rate of plants.

The invention relates to the technical field of bioengineering, and discloses a mutant of Burkholderia lipase as well as a recombinant expression vector and application of the mutant, the mutant comprises P131S, T132A, T251A, T251S, P131A, P131Q, T132G, Q88S and P113A single processes; and a Q88S double process and a P113A double process; and a multi-site mutation combination: a combination I: Q88S, P113A, T132G and L287S, and a combination II: Q88S, P113A, T132G and L287S; a combination II: Q88S, P113A, P131S and L287S; and a combination III: Q88S, P113A, P131S and T251A. Based on experiments, the invention finds that each mutant can not only improve the protein yield, but also improve the enzyme activity by 57% at most; ePA enrichment experiments find that the EPA content of the mutant can be increased by 33% at most, and the mutant has excellent EPA purification and enrichment effects.

The invention discloses a tyrosine phenol lyase mutant and application of the tyrosine phenol lyase mutant in synthesis of levodobar. The tyrosine phenol lyase mutant is obtained by carrying out site-specific mutagenesis on the basis of wild type tyrosine phenol lyase. The mutant obtained through transformation can transform more substrates, the yield is also obviously improved, and the method is suitable for industrial production of levodopa.

The invention discloses a methyl paraoxon pesticide detection test strip based on oxygen vacancy-cerium dioxide and a preparation method of the methyl paraoxon pesticide detection test strip, and belongs to the technical field of biosensors. The test strip is characterized in that a large amount of oxygen vacancy-cerium dioxide is loaded. The oxygen vacancy-cerium dioxide nano enzyme can show simulated phosphatase activity under specific conditions, and the enzyme activity can cut off P-O and P-S bonds of organophosphorus pesticides and promote methyl paraoxon to be hydrolyzed into a yellow product p-nitrophenol. The detection limit of a nano-enzyme system is discussed, so that the portable visual quantitative detection of the paraoxon methyl pesticide is realized. The test strip established on the basis of the principle can quickly detect paraoxon methyl, has the advantages of simplicity and convenience in operation, high sensitivity, low cost and the like, and provides a new method for instant test of paraoxon methyl pesticide.

The invention relates to the field of gene engineering and enzyme engineering, in particular to an acid protease mutant with improved stability and a product and application thereof. In particular to an acid protease mutant IAIC6M with improved heat resistance and a coding gene thereof. A mutant IAIC6M is obtained by performing D86I, G168A, Q185I and Q339C mutation on an iaic6 sequence as shown in an amino acid sequence SEQ ID NO.1, and compared with IAIC, the mutant can still maintain more than 60% of enzyme activity at 75 DEG C for 3 minutes, while the IAIC can only maintain 20% of enzyme activity at 75 DEG C for 3 minutes; the acid protease mutant IAIC6M disclosed by the invention has the following properties: the optimum pH is 3.0, and the acid protease mutant IAIC6M still has higher enzyme activity under a high-temperature condition and has wide application value in the aspects of industry, food, medicine and the like.

The invention belongs to the technical field of microorganisms, and relates to lactobacillus plantarum SCWLac-1 and application thereof in aquaculture, in particular to application in the aspect of litopenaeus vannamei culture. The strain can be fermented on a large scale and can promote digestion and absorption of aquaculture animals so as to promote growth; in addition, the compound has a relatively strong inhibition effect on pathogenic bacteria, and the non-specific immune enzyme activity of the litopenaeus vannamei can also be improved; the microbial agent prepared from the strain and the aquaculture feed containing the strain are applied to aquaculture, diseases in aquaculture can be reduced, the use of antibiotics is indirectly reduced, the aquaculture environment is improved, and the economic benefits of farmers are further improved.

The invention belongs to the field of bioengineering, and particularly relates to phosphorylcholine cytidyltransferase and application thereof in preparation of citicoline, and the specific application is that the phosphorylcholine cytidyltransferase can efficiently catalyze phosphorylcholine and CTP to react to generate citicoline. The phosphorylcholine cytidyltransferase is prepared by the following steps: synthesizing a coding gene of the phosphorylcholine cytidyltransferase, selecting pHT01 as an expression vector, co-expressing with choline kinase, transforming into host bacteria, and inducing protein expression. According to the choline phosphate cytidyltransferase disclosed by the invention, under the same reaction condition and within the same reaction time, the yield of a citicoline product obtained by conversion is higher than that of choline phosphate cytidyltransferase (ScCCT) which is most commonly used in the market at present and is derived from saccharomyces cerevisiae S288c. Specifically, the yield of the MsCCT001 is 10 times higher than that of the ScCCT, the yield of the MbCCT006 is 6 times higher than that of the ScCCT, and the yield of the MeCCT008 is 8 times higher than that of the ScCCT.

The invention discloses a glutamate decarboxylase multi-site combined mutant and application thereof, and relates to the technical field of enzyme engineering and gene engineering. Amino acid residue sites needing to be mutated are determined on the basis of amino acid sites near key amino acids of a coenzyme 5 '-phosphopyridoxal binding pocket, and experimental verification is performed through a site-directed mutagenesis technology in combination with a high performance liquid chromatography screening method. Compared with a chemical synthesis method, the compound is synthesized by utilizing glutamic acid decarboxylase through biological catalysis, the synthesis condition is mild, the operation is simple, and separation and extraction are easy. According to the method, the screening probability of mutant amino acid sites can be effectively improved, the experiment efficiency and feasibility are improved, and the mutant enzyme of which the enzyme activity is obviously superior to that of the parent enzyme is obtained through screening.

The invention relates to a method for improving soil fertility and enzyme activity of a rice field by using a nitrogen-fixing cyanobacteria biological membrane, which comprises the following steps: step 1, expanding propagation of nitrogen-fixing cyanobacteria: selecting anabaena globifera as a rice field application algae species, and performing expanding culture on the algae species to obtain a nitrogen-fixing cyanobacteria secondary algae species; step 2, applying nitrogen-fixing cyanobacteria: applying the nitrogen-fixing cyanobacteria in a rice base fertilizer period and a tillering period, and replacing part of chemical fertilizer with the nitrogen-fixing cyanobacteria to reduce the application amount of the chemical fertilizer; secondly, the nitrogen-fixing cyanobacteria returns to the rice field through biological nitrogen fixation and rotting decomposition of dead algae, the content of nitrogen and carbon in the soil is increased, the soil fertility is improved, and the nitrogen-fixing cyanobacteria can provide nutrient substances for microorganisms in the soil, improve the enzyme activity of the soil and improve the quality of the soil when applied to the rice field.

The invention belongs to the technical field of soil conservation, and particularly relates to a method for promoting plant growth and regulating soil nutrients. The invention discloses a method for regulating and controlling corn and tobacco growth and rhizosphere soil nutrients through combination of AMF and a planting mode. The method comprises the following steps: preparing an AMF-containing microbial inoculum by using M. mosseae (F. mosseae); cultivating flue-cured tobacco seedlings and corn seedlings; flue-cured tobacco seedlings and corn seedlings are jointly planted in an intercropping mode by utilizing an AMF-containing fungicide. The method can relieve continuous cropping obstacles of flue-cured tobaccos and promote growth of the flue-cured tobaccos and corn, and has a certain effect on soil conservation.

The invention discloses an ethylene chain-based ferrocenyl covalent organic polymer and application thereof, and belongs to the technical field of biological medicines. The covalent organic polymer based on the ethylene chain ferrocenyl is a covalent organic polymer which is obtained by taking 1, 1 '-ferrocenedicarboxaldehyde, bromoethane and 2, 4, 6-trimethyl-1, 3, 5-triazine as monomers through polymerization and has a completely conjugated structure. The method comprises the following steps: adding 1, 1 '-ferrocenedicarboxaldehyde, bromoethane and 2, 4, 6-trimethyl-1, 3, 5-triazine into a mixed solvent under a protective atmosphere, uniformly stirring, carrying out solvothermal reaction in a closed environment, and washing and drying a product obtained by filtering to obtain the covalent organic polymer based on ethylene chain ferrocenyl. The novel COP material ITAFc-COP with a completely conjugated structure is synthesized, the photo-thermal activity of the novel COP material ITAFc-COP is enhanced by the completely conjugated structure of ITAFc-COP, and the novel COP material has a photo-thermal effect and pod-like activity at the same time.

The invention discloses a mutant with improved enzyme activity of sucrose isomerase and application of the mutant, and belongs to the technical field of genetic engineering and enzyme engineering in molecular biology. A known sucrose isomerase parent is subjected to molecular modification by utilizing a site-directed mutagenesis technology to obtain mutant enzymes T522S / K340D, D222H / K340D, G467N / K340D, T522S, K340D, D222H and G467N, and the catalytic activity of the series of mutants provided by the invention is increased by 10%-25% compared with that of original bacteria; the isomaltulose conversion rates of the combined mutants T522S / K340D, D222H / K340D and G467N / K340D are respectively increased by 2.3%, 1.5% and 1.3% compared with those of original bacteria, and the strain has good stability, can be used for biological preparation of isomaltulose and has potential application prospects.

The invention discloses aminopeptidase for synthesizing L-carnosine and an application of the aminopeptidase. The amino acid sequence of the aminopeptidase is as shown in SEQ ID NO: 3 or 5. The aminopeptidase disclosed by the invention can catalyze L-histidine with high selectivity to synthesize L-carnosine in the presence of beta-alanine methyl ester, and has relatively high enzyme activity and industrial application value.

The invention discloses efficient synthesis of ursodesoxycholic acid by improving the positive enzyme activity of 7 beta-hydroxysteroid dehydrogenase through directed evolution, and belongs to the field of biological catalysis engineering. According to the invention, the 7beta-hydroxysteroid dehydrogenase mutant TEAE / F152L / W101N with improved positive reaction activity and improved affinity with a substrate is obtained. Compared with TEAE, the forward enzyme activity of the mutant enzyme is improved by 2.9 times, the reverse activity of the mutant enzyme is reduced by 3.6 times, the catalytic efficiency kcat / Km of the mutant enzyme to 7K-LCA is improved by 4.3 times, and compared with a wild type mutant TEAE / F152L / W101N, the enzyme activity of the mutant enzyme is improved by 206.23 times, and the catalytic efficiency of the mutant enzyme to 7K-LCA is improved by 58.56 times. The invention provides a universal technical platform for directed evolution of hydroxysteroid dehydrogenase, provides an excellent mutant enzyme for biological efficient synthesis of ursodesoxycholic acid, and lays a solid research foundation for efficient and low-cost preparation of ursodesoxycholic acid.

The present invention relates to a microbial agent for preventing and treating root rot of golden olive and its application. The microbial agent comprises the following components: Bacillus velezcema with a preservation number of CCTCC No: M2022923 ( Bacillus velezensis ), Bacillus subtilis with a deposit number of CCTCC No: M20221207 ( Bacillus subtle ), marigold extract, amino acids, humic acid and trace elements. The microbial agent can significantly improve the physical and chemical properties of the soil for the cultivation of golden olives, increase enzyme activity and microbial diversity, reduce the content of phenolic acid and Fusarium toxins in the soil, and thus reduce the Fusarium nightshade ) and oomycetes ( Pythium aphanidermatum ) and ultimately achieve the prevention and control of golden olive root rot.

The invention discloses a construction method of a heparin 2-O-sulfotransferase high-activity mutant, and belongs to the technical field of biology. According to the heparin 2-O-sulfotransferase and the application of the heparin 2-O-sulfotransferase, a dissolution promoting label is fused at the N end of the heparin 2-O-sulfotransferase, the coding sequence of the dissolution promoting label is optimized, and active expression of the heparin 2-O-sulfotransferase is achieved; according to the invention, the heparin 2-O-sulfotransferase is mutated, so that a mutant with high stability and catalytic activity is obtained; furthermore, fermentation conditions of the heparin 2-O-sulfotransferase are optimized, so that the expression quantity and the enzyme activity are improved, and the application potential of the heparin 2-O-sulfotransferase in industrial production is enhanced.

The invention discloses cyclohexene formate esterase, a mutant of cyclohexene formate esterase and application of cyclohexene formate esterase in production of (S)-3-cyclohexene-1-formic acid. According to the cyclohexene formate esterase and the mutant disclosed by the invention, 100-400g / L of racemic 3-cyclohexene-1-methyl formate can be split by taking wet thalli subjected to induced expression or crude enzyme liquid obtained by ultrasonically crushing the wet thalli as a catalyst, so that (S)-3-cyclohexene-1-formic acid with chemical purity and optical purity reaching 99% or above is synthesized. The enzyme activity of the cyclohexene formate esterase mutant provided by the invention is further improved, the reaction time and the catalyst dosage are obviously reduced compared with those of a substrate with the same catalysis concentration before mutation, the reaction condition is mild, the operation is relatively simple and convenient, and the cyclohexene formate esterase mutant has a very good industrial application prospect.

The invention discloses a multifunctional nano-enzyme as well as a preparation method and application thereof, and belongs to the technical field of nano-enzyme synthesis. Aiming at the problems that a traditional nano-enzyme is single in activity, limited in treatment effect, poor in tumor active targeting specificity and the like, the multifunctional nano-enzyme provided by the invention is simple and efficient in preparation method, and is prepared by taking N and P double-doped hollow carbon nanospheres as a carrier, loading L-arginine and glucose oxidase and taking an aptamer as a targeting molecule. The multifunctional nano-enzyme is small in particle size, high in dispersion degree and good in biological safety. The multifunctional nano-enzyme not only has the activities of peroxidase, nitric oxide synthase and glucose oxidase, but also has excellent photo-thermal conversion capacity in tumor targeted therapy. The multifunctional nano-enzyme provided by the invention has a tumor focus specific recognition capability, can be highly enriched at a tumor part, finally realizes the purpose of photothermal / catalytic treatment combined tumor treatment, and provides a new thought and platform for tumor treatment.

The invention belongs to the technical field of biological fertilizers, and particularly provides a carbon sequestration and nitrogen conservation biological organic fertilizer and a preparation method thereof.Molybdenum, copper, cobalt and composite flora are loaded on biomass charcoal to prepare load type biomass charcoal; and jointly preparing the supported biomass charcoal, the perilla biomass extracting solution and animal waste into the biological organic fertilizer. According to the biological organic fertilizer provided by the invention, unnecessary waste of strain resources can be avoided, and the organic fertilizer can remarkably promote the immobilization effect of soil on ammonium nitrogen and nitrate nitrogen; the compound bacteria liquid promotes decomposition of organic matters and effectiveness of nutrients, and promotes improvement of soil, so that improvement of soil productivity is facilitated; meanwhile, the organic fertilizer can obviously improve the enzyme activity of the soil and promote the improvement of the fertility of the soil, so that the effect of conditioning the soil is finally realized.

The colla corii asini low-peptide molecule generator comprises a supporting plate and a motor, a rotating mechanism is arranged at one end of the side face of the motor, and an enzymolysis tank used for mixing a colla corii asini aqueous solution and enzymes is arranged at one end of the side face of the rotating mechanism; one end of the side surface of the enzymolysis tank is provided with an air extracting mechanism for extracting air from the interior of the enzymolysis tank, and a first connecting pipe and a second connecting pipe for air circulation are arranged between the air extracting mechanism and the enzymolysis tank; according to the device, oxygen in the enzymolysis tank can be pumped out through the air pumping mechanism when an oxygen-free environment is needed in the enzymolysis process, the enzymatic activity is protected, the enzymolysis efficiency can be better improved, then a donkey-hide gelatin aqueous solution and enzymes in the enzymolysis tank can be more uniformly mixed through the rotating mechanism, the enzymolysis tank and the like, and the enzymolysis efficiency is improved. The enzymolysis efficiency is improved, and the quality of protein is ensured.

The invention provides an improvement method for improving soil porosity of saline-alkali soil, which comprises the following steps: (1) uniformly spreading a saline-alkali soil conditioner on the surface of saline-alkali soil to be improved, and uniformly mixing the saline-alkali soil conditioner with the saline-alkali soil; (2) ploughing the saline-alkali soil to obtain a plough layer; (3) flattening the surface of the saline-alkali soil, and thoroughly watering the saline-alkali soil with water; after watering is conducted for 15-20 days, the saline-alkali soil is turned over again, and a planting layer is obtained; the soil conditioner in the step (1) is prepared from 20 to 30 parts of attapulgite-polyacrylamide, 10 to 30 parts of charcoal, 20 to 30 parts of humic acid and 10 to 20 parts of ardealite. Through the mass ratio of the attapulgite-polyacrylamide to the biochar to the humic acid, the obtained soil conditioner can better repair soil and can better maintain the granular structure of the soil, and the water retention and fertilizer retention effects are achieved.

The invention provides a bacillus for producing cellulase and application thereof, the bacillus strain XWSM3 is separated from soil of sugarcane field of Guangxi guest, the preservation number of the bacillus strain XWSM3 is GDMCC NO: 65137, the preservation date is September 14, 2024, the preservation address is Microbial Culture Collection Center in Guangzhou and Guangdong province, the preservation unit is Guangdong province Microbial Culture Collection Center, the preservation number is CGMCC NO: 65137, the preservation number is CGMCC NO: 65137, the preservation number is CGMCC NO: 65137, the preservation number is CGMCC NO: 65137, and the preservation number is CGMCC NO: 65137. The strain has relatively high enzyme production activity and good cellulase production stability, the enzyme activity of the enzyme produced by the strain reaches 16.53 U, and the yield of the prepared humic acid reaches 7.10%.

The invention belongs to the field of biopharmacy and biotransformation, and particularly relates to a nicotinamide mononucleotide adenosine transferase mutant and application thereof. According to the invention, NMN is taken as a substrate, ATP disodium salt is taken as an auxiliary substrate, and NAD < + > is prepared under the catalysis of the nicotinamide mononucleotide adenosine transferase mutant under the action of magnesium ions, so that the enzymatic activity is improved, few byproducts are generated, and the reaction rate is high. The nicotinamide mononucleotide adenosine transferase mutant disclosed by the invention has better catalytic efficiency, and when NMN is taken as a substrate for catalytic reaction, the conversion rate is higher than that of an original enzyme, and the NAD < + > yield is also increased to a certain extent. The process overcomes the defects of various process steps, harsh reaction conditions, difficulty in separation and purification and the like of a chemical method for synthesizing NAD < + >, and is an efficient, low-cost and environment-friendly production mode.

The invention belongs to the technical field of gene engineering, and particularly relates to an IsMHETase mutant with high catalytic activity and application of the IsMHETase mutant. The invention aims to solve the technical problem that the activity of the existing ISMETase is relatively low. The technical scheme of the invention is as follows: the IsMHETase mutant with high catalytic activity is a protein obtained by mutating IsMHETase of which the amino acid sequence is as shown in SEQ ID No.1. The invention also discloses a preparation method of the IsMHETase mutant with high catalytic activity. Six mutants are obtained after screening, and the enzyme activity is improved by 1.13-3.20 times compared with that of a wild type IsMHETase; the IsMHETase mutant obtained by the invention has higher enzyme activity, is more efficient when being used for biodegrading MHET micromolecules, simplifies the degradation steps, reduces the degradation cost, and provides a wide prospect for the application of the IsMHETase mutant in the industrial field.

The invention belongs to the technical field of biology, and particularly relates to a small molecule peptide composition and method for regulating natural calculus bovis production. The small molecule peptide aiming at a key target spot is designed by deeply researching secretion and metabolism of ox bile. The action mechanism covers biliary secretion stimulation, component optimization, bezoar key enzyme activity promotion and immune response regulation. A chemical synthesis or biotechnology means is adopted for preparation, and the dosage is determined according to cattle conditions during application. The safety is strictly evaluated, the yield of the natural calculus bovis can be obviously improved by 65%, the quality is also greatly improved, the effective components are increased, the impurities are reduced, the drug effect is enhanced, a new efficient and safe way is opened up for the production of the natural calculus bovis, and the method has a wide application prospect.

The invention discloses an irrigation method for improving the water and fertilizer utilization rate of greenhouse tomatoes, and the method adopts a method of combining a circulating aeration device with drip irrigation to control the fertilization amount, the soil water content and the irrigation amount in each period of tomato growth so as to monitor the change response of the soil environment and the migration, transformation and regulation of nutrients in a tomato root zone. By adopting an irrigation fertilization mode with a high aeration irrigation medium fertilizer level, growth of tomato plants and dry matter accumulation are promoted, root morphology distribution is improved, root activity is improved, photosynthetic indexes and chlorophyll content of the tomato plants are remarkably improved, photosynthesis is enhanced, and fruit yield, transverse diameter, longitudinal diameter and fruit quality are remarkably improved; the soil enzyme activity and the microorganism quantity of the tomato root zone are obviously improved, and the nutrient absorption accumulation amount and the nutrient absorption and utilization efficiency of tomato plants are improved.

The application provides an arginine deiminase mutant with alkali resistance, and the amino acid sequence of the mutant is SEQ ID NO:3. When L-arginine is used as a substrate to synthesize L-citrulline, the pH value of a reaction system can be as high as pH 11 or higher, and hydrochloric acid neutralization is not needed, so that the cost of product separation and purification and waste water treatment is significantly reduced.

The application belongs to the technical field of bioengineering, and particularly relates to a xanthan gum cleavage ancestral enzyme AncXLY196 based on ancestral sequence reconstruction, an amino acid sequence of which is shown as SEQ ID NO. 2. Based on the xanthan gum cleavage ancestral enzyme, a mutant AncXLY196-X7 is further obtained, an amino acid sequence of which is shown as SEQ ID NO. 4, xanthan gum degradation activity of which reaches 2.5 times of that of the xanthan gum cleavage ancestral enzyme, and the optimal catalytic temperature of the xanthan gum cleavage ancestral enzyme and the mutant thereof is increased to 45 DEG C, and the enzyme activity of the mutant of the xanthan gum cleavage ancestral enzyme is reserved by 50-80% after being treated at 45-55 DEG C for 12-24 h. The application provides a new enzyme resource for efficient enzymatic degradation of xanthan gum and preparation of functional oligosaccharides.

The invention discloses a carbonyl reductase mutant, an immobilized carbonyl reductase and its application, and relates to the field of genetic engineering and enzyme engineering technology. A carbonyl reductase mutant, whose amino acid sequence is shown in SEQ ID NO.1; a carbonyl reductase mutant crude enzyme solution is mixed with chitosan microspheres, a glutaraldehyde solution is added dropwise, oscillation cross-linked, and washed with water to obtain an immobilized carbonyl reductase. The enzyme activity of the immobilized carbonyl reductase prepared in the present application is increased by 5-10 times compared with that of the wild type. When eslicarbazepine is prepared by asymmetric reduction catalyzed by immobilized carbonyl reductase, the substrate concentration reaches 80-150 g / L, the chiral purity value is greater than 99%, and the conversion rate is greater than 99%. The immobilized enzyme of the present invention can be recovered by simple filtration, can be repeatedly used more than 5 times after recovery, and the chiral purity and conversion rate of the reaction used for the first time are substantially the same when reused, and has a high potential for industrial application.