3333results about How to "Increase enzyme activity" patented technology

Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.

A bio-implantable electrochemical cell system for active implantable medical devices. In one embodiment, the fuel cell includes an electrode structure consisting of immobilized anode and cathode enzymes deposited on nanostructured high-surface-area metal nanowires or carbon nanotube electrodes. The anode enzyme comprises immobilized glucose oxidase and the cathode enzyme comprises immobilized laccase. Glucose is oxidized at the surface of the anode and oxygen is reduced at the surface of the cathode. The coupled glucose oxidation-oxygen reduction reactions provide a self-generating current source. In another embodiment, the nanowires or carbon nanotubes, along with the adjacent surface anode and cathode electrodes, are coated with immobilized glucose oxidase and immobilized laccase containing biocolloidal substrates, respectively. This results in the precise construction of an enzyme architecture with control at the molecular level, while increasing the reactive surface area and corresponding output power by at least two orders of magnitude.

The invention relates to a fermentation method for production of nutritionally balanced natural fruit enzymes. The method comprises the following steps: (1) pretreatment on raw materials; (2) a first bacterium inoculation: inoculating a saccharomyces cerevisiae liquor with concentration of 106 CFU / ml into a pulp according to an inoculation proportion of 5%; (3) early stage fermentation; (4) a secondary bacterium inoculation: after the early fermentation, inoculating acetic acid bacterium liquid and lactic acid bacterium liquid in a weight ratio of 1:1 for a secondary bacterium inoculation according to an inoculation amount of 5%; (5) middle-term fermentation; (6) coarse filtration; (7) secondary fermentation; (8) ripening; and (9) fine filtration to obtain the natural fruit enzyme. The fermentation method provided by the invention through low-temperature sterilization and strict control on the fermentation temperature retains the enzyme activity to the maximum; and twice inoculation of dominant bacteria effectively inhibits breeding of other infectious microbes, so as to realize stable product quality.

The present invention relates to a fruit and vegetable ferment beverage and a preparation method thereof. The beverage is prepared by using carrots, shii-takes, burdock roots, prunes, pineapples, mulberries, beta vulgaris and tomatoes as main raw materials, sorting, cleaning, drying and squeezing the raw materials, fermenting the squeezed raw materials with the addition of yeast, vinegar organism and lactic acid bacteria, and adding drinking water, brown sugar, isomaltooligosaccharide and L-malic acid as adjunct materials into the mixture. The beverage uses fruit and vegetable nutrient components of the raw materials for mutual promotion and mutual complementation to synergistically improve nutritional value of the whole beverage, and is fermented with three kinds of bacteria so that fruit and vegetable nutrients and beneficial metabolites of probiotics are preserved, making the beverage has effects in conditioning stomach and intestine, improving digestion and absorption, strengthening physical fitness, enhancing immunity, improving resistance, eliminating free radicals, delaying aging, preventing cancers, maintaining beauty and preserving countenance and controlling weight.

The invention provides a soil improvement type straw compound organic fertilizer and a preparation method of the soil improvement type straw compound organic fertilizer. The soil improvement type straw compound organic fertilizer is prepared from the following raw materials in parts by weight: 30 to 35 parts of rice straw, 25 to 30 parts of animal excrement, 20 to 25 parts of biological charcoal, 10 to 15 parts of soybean meal, 5 to 10 parts of cassava residue, 5 to 10 parts of diatom ooze, 2 to 2.5 parts of plant ash, 2 to 2.5 parts of turfy soil, 2 to 3 parts of zeolite powder, 3 to 4 parts of a microorganism bacterium agent, 2 to 3 parts of sodium benzoate, 2 to 3 parts of ammonium monohydrogen phosphate, 1 to 2 parts of calcium chloride, 1 to 2 parts of amino acid, 1 to 2 parts of starch, 1 to 2 parts of molasses powder and 1 to 2 parts of humic acid. The soil improvement type straw compound organic fertilizer takes plant wastes as raw materials; the quality of soil can be easily improved and the production cost can also be reduced, so that the physicochemical properties of the soil are improved, and the structure of the soil is comprehensively improved, and furthermore, the soil quality has the effects of softness, air permeability, water retention and fertilizer conservation; the aim of producing an environment-friendly high-quality organic fertilizer through preferably selecting heavy metal capable of being passivated, the biological charcoal capable of improving the fertilizer efficiency of the organic fertilizer, and conversion products of organic wastes including the biological humic acid and the like as composting base materials, and optimizing a composting process.

A process for improving the yield and efficiency of an ethanol fermentation plant that receives organic fermentable feedstock material, prepares the feedstock for fermentation, ferments the feedstock with yeast to produce ethanol, and produces stillage as a byproduct of ethanol fermentation. The process steps which can be operated independently or in combination, may include, but are not limited to, degrading fatty acids in the fermentable feedstock material prior to fermentation; degrading cellulose and hemicellulose present in the feedstock prior to fermentation; adding a surfactant to the fermentable feedstock; separating a liquid fraction from the stillage; recycling the liquid fraction to be combined with the fermentable feedstock; recovering a solid fraction from the stillage; and introducing at least a portion of the solid fraction to an anaerobic digester to produce methane.

The present invention relates to a method for generating alkenes biologically. It relates more particularly to a method for producing terminal alkenes by enzymatic decarboxylation of 3-hydroxyalkanoate molecules. The invention also relates to the enzymatic systems and the microbial strains used, and also to the products obtained.

The invention relates to preparation method and application method of a grape special fertilizer, wherein the preparation method comprises the following steps: preparing an I-type fertilizer by taking the following ingredients in parts by weight: 30-40 parts of urea, 20-30 parts ammonium phosphate, 15-30 parts of potassium sulphate, 1-3 parts of humic acid, 1-3 parts of excrement, 1-5 parts of synergist, 0.1-1.0 part of ferrous sulphate, 0.1-0.8 part of zinc sulfate, 0.1-0.6 part of manganese sulphate, 0.1-0.6 part of borax, 0.1-0.4 part of copper sulfate, 3-7 parts of plaster and 3-13 parts of bentonite; preparing an II-type fertilizer by taking the following ingredients in parts by weight: 7-15 parts of urea, 25-35 parts ammonium phosphate, 35-45 parts of potassium sulphate, 1-3 parts of humic acid, 1-5 parts of excrement, 2-5 parts of synergist, 0.1-1.0 part of ferrous sulphate, 0.1-0.8 part of zinc sulfate, 0.1-0.6 part of manganese sulphate, 0.1-0.6 part of borax, 0.1-0.4 part of copper sulfate, 3-7 parts of plaster and 3-13 parts of bentonite; and blending, spraying, pelleting and drying at lower temperature to obtain the product. The application method of the fertilizer comprises the following steps: taking an organic fertilizer and the II-type fertilizer as a base fertilizer; applying the I-type fertilizer before blossom, applying the II-type fertilizer in the young fruit development period, and meanwhile, respectively spraying monopotassium phosphate and rare earth mixed liquid once on the leaf surfaces; and spraying calcium nitrate or calcium acetate to enhance the storage resistance property before picking.

The present invention relates to methods of producing modified fatty acids comprising a functional group which is a hydroxyl group, an epoxy group, an acetylenic group or a conjugated double bond. For example, seeds, seedoil and methods of making seedoil are provided wherein at least 23% (mol %) of the fatty acid content of the seed or seedoil comprises the functional group. Also provided are novel polypeptides, and polynucleotides thereof, which can be used to produce the modified fatty acids, particularly in transgenic plants and cells suitable for fermentation.

Method and apparatus for processing paraffin embedded samples, e.g., to disassociate paraffin from tissue components and / or other biomolecules from the paraffin. The sample may be exposed to focused acoustic energy while held in a vessel containing a non-solvent, aqueous solution. Disassociated paraffin may be emulsified into the liquid or otherwise separated from the sample.

The invention discloses an anti-stress anti-diarrhea growth-promotion Chinese herbal medicine feed additive for a weaned pig, which is obtained by carrying out the superfine grinding and mixing on the following components at the mass ratio: 1-5 parts of Astragalus mongholicus, 1 part of Scutellaria baicalensis, 1 part of malt, 1 part of liquorice, 1-5 parts of dangshen, 1-5 parts of poria cocos and 1-5 parts of largehead atractylodes rhizome. According to the Chinese herbal medicine feed additive disclosed by the invention, the weaned pig growth performance can be improved, and the Chinese herbal medicine feed additive has the anti-diarrhea effect; the gastrointestinal tract development of the weaned pig can be promoted; the activity of the digestive enzyme of the gastrointestinal digestive tract of the wigged pig can be improved to promote the development of the pig digestive enzyme system; the micro-ecological environment of the digestive tract of the pig can be regulated and stabilized to accelerate the pig to grow; the antioxidation function of the wigged pig organism can be improved so as to improve the compensatory capability of the wigged pig organism on external irradiation; the intestinal mucosa form of the wigged pig can be improved so as to alleviate the intestinal villus withering of the wigged pig and promote the tract of the wigged pig to restore; and the immunologic function of the intestinal mucosa of the wigged pig can be regulated.

The invention relates to a magnetic gamma-Fe2O3 nano-particle mimetic enzyme and provides a method for applying a magnetic gamma-Fe2O3 nano-particle mimetic enzyme to biological detection. The magnetic gamma-Fe2O3 nano-particle mimetic enzyme replaces a horse radish peroxidase (HRP) to be used in biological detection. The method comprises the following steps: coupling carboxyl groups on the surface of magnetic gamma-Fe2O3 nano-particles and a specific molecular probe to construct a specific nano probe; combining the nano probe with the specificity of the corresponding target molecules to be detected; developing by using a developer containing peroxides and hydrogen-supply zymolytes; and measuring the absorbency or carrying out the micro-observation so as to realize the qualitative and quantitative detection on target molecules.

The invention discloses an aspergillusniger strain for producing beta-glucuroide. Aspergillusniger T2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on 26th September, 2008 with the preservation number of CGMCC No.2715. The aspergillusniger T2 strain is the aspergillusniger strain which is obtained by performing mutagenesis with ultraviolet rays and nitrous acid and screening by using a Congo red-carboxymethylcellulose (CMC) double-layer flat plate, has high enzymatic activity and is used for producing the beta-glucuroide. The aspergillusniger T2 strain is applied to the production of the beta-glucuroide. The beta-glucuroide produced by performing liquid state fermentation on corn cob, yeast powder and the like serving as raw materials and the aspergillusniger T2 strain serving as the strain has the characteristics of enzymatic activity, simple production method and the like.

The invention relates to a method for biosynthesis preparation of human glucagon-like peptide-1 (GLP-1) and an analogue thereof. With adopting of a gene engineering technology, a recombinant escherichia coli expressed GLP-1 fusion protein is constructed, and a protein enzyme digestion site is designed in the fusion protein; a fusion gene has a gene sequence with a form of A-B-C structure, wherein A is a chaperonin gene, B is a nucleotide sequence encoding a connection peptide containing the enzyme digestion site, and C is a gene encoding the GLP-1 or the analogue thereof. After recombinant engineering bacteria is subjected to induced expression, the fusion protein is purified and subjected to enzyme digestion, and then the GLP-1 and the analogue thereof are obtained and are detected to have biological activity. The preparation method of the GLP-1 and the analogue thereof provided by the invention is simple and quick, the production conditions are mild, the product is convenient to separate and extract, the process is simple, and the industrialization prospect is good.

The invention provides a transaminase mutant and applications of the transaminase mutant. The amino acid sequence of the transaminase mutant is the amino acid sequence with mutation as shown in SEQ IDNO:1, the amino acid sites with mutation comprise the T7C+S47C site. For the transaminase mutant with the mutation sites, the enzyme activity and / or stability are / is greatly improved, and the transaminase mutant can be applied in the relatively extreme environment. In addition, the transaminase mutant with the mutation sites can be further prepared into immobilized enzyme through the immobilization technology, the activity and the stability of the immobilized enzyme are relatively high, and the immobilized enzyme can be recycled for a plurality of times, and is suitable for being applied to continuous flow reaction of a packed bed.

The invention relates to a bacillus subtilis subso natto for producing natto kinase and application thereof. The bacillus subtilis subso natto (bacillus subtilis subso natto LNUB236), CCTCC No.: M 208156. The bacillus subtilis subso natto is subject to activation of culture, seed liquid and fermentation liquor are prepared, and the natto kinase is extracted. The invention relates to application of the natto kinase to preparing a health product for preventing and treating cerebral thrombosis. Constituents of the health product are proportioned by weight: lyophilized natto powder : natto kinase : natto kinase : evening primrose oil=1:2.0-3.0:1.0-1.5. The bacillus subtilis subso natto LNUB236 has high natto kinase yield, the highest enzyme activity is 431.455IU / ml in the event of production in a 30l fermenter, and the prepared natto kinase-containing health product has the advantages of high enzyme activity, obvious effect and no side effect.

The invention relates to the field of malting and in particular relates to a method for preparing malts with high malt aroma and beer thereof. The method mainly comprises the following steps: selecting wheat, raw material pretreatment, steeping, sprouting, green malt drying, kilning and removing roots. According to the steeping and sprouting process, the sprouting uniformity is improved, lots of amino acids and reducing sugars are generated under the optimum water activity conditions, the degree of a Maillard reaction is controlled through the drying and gradient temperature rise kilning process, specific malt aroma components are generated, and due to sensory evaluation, the malt has pure and strong aroma, is clean and coordinative and does not have burnt taste and other odors. The method is used for improving the content of malt aroma substances in the malt, the defects that the malt prepared in the prior art is not obvious in aroma and partial specific malts have obvious burnt taste except certain malt aroma are overcome, the technical problem that the malt is aromatic but easily burnt is effectively solved, pure and strong malt aroma is presented, the malt can be used for preparing high-malt aroma wort or high-malt aroma beer, and the typical flavor characteristics of the malt aroma of the beer are reflected.

The invention relates to a method for preparing a mixed-type nucleotide feed additive from yeast cells and applications of the feed additive, which belong to the technical field of the preparation of nucleotide feed additives from yeast cells. The method comprises the steps: using yeast cells as raw materials and combining a homogenization method and a salt method to break yeast cell walls ; separating the yeast cell walls, protein and other insoluble substances by centrifugation and fractional isoelectric crystallization; specially collecting RNA precipitates at special isoelectric points to obtain wet and crude nucleic acid with the purity of 70 percent; dissolving the wet and crude nucleic acid in water, using high-activity phosphodiesterase from malt roots to carry out a high-efficiency directional enzymatic reaction; directly spraying, drying and crushing the solution after the enzymatic reaction to obtain the mixed-type nucleotide feed additive product comprising the protein content lower than 10 percent and four monosomic nucleotides (AMP, GMP, CMP and UMP) larger than 60 percent. After the feed additive is applied to animal feed, the intestinal absorption function of animals can be notably improved, the growth of the animals is stimulated, and the immune function, the antiviral capability and the anti-stress capability of the animals are improved.

The invention provides a clothes washing and drying machine. The clothes washing and drying machine comprises a wash bucket assembly and a heat pump assembly, the wash bucket assembly comprises a water inlet heat exchanger, a detergent box and a wash bucket, the water inlet end of the water inlet heat exchanger is connected with a water inlet pipeline, the water outlet end of the water inlet heat exchanger is connected with the water inlet end of the detergent box, and the water outlet end of the detergent box is connected with the wash bucket. The heat pump assembly comprises a heating condenser arranged inside the water inlet heat exchanger. According to the clothes washing and drying machine, the water inlet heat exchanger is arranged, and cold water inside the water inlet heat exchanger is heated through the heating condenser of the heat pump assembly. Compared with an electrical heating mode in the prior art, energy consumption can be effectively reduced, the water inlet heat exchanger is arranged on the upper stream of the detergent box, dissolution of detergent is facilitated, the activity of enzyme in the detergent is improved, and therefore the clothes washing effect is better.

Compositions comprising DNase encapsulated in a liposome are provided. Additionally, provided are compositions comprising a free enzyme and empty liposomes, compositions comprising a free enzyme and an antiinfective encapsulated in a liposome, compositions comprising a free enzyme, a free-antiinfective, and empty liposomes, compositions comprising a free enzyme, a free antiinfective, and an antiinfective encapsulated in a liposome, and a composition comprising a free enzyme, empty liposomes, and an antiinfective encapsulated in a liposome, and compositions comprising a free enzyme, a free antiinfective, empty liposomes, an antiinfective encapsulated in a liposome, and pharmaceutical compositions comprising the aforementioned compositions. The Also provided are methods of treating pneumonia, bronchitis, cystic fibrosis, or emphysema comprising administering to a subject in need thereof a therapeutically effective amount of the aforementioned pharmaceutical composition.

The present invention discloses genetically-modified cyanobacteria with ethanol-production capabilities enhanced over the currently-reported art, and methods of making such cyanobacteria. The invention provides a genetically modified photoautotrophic, ethanol producing host cell comprising an overexpressed pyruvate decarboxylase enzyme converting pyruvate to acetaldehyde and an overexpressed Zn2+ dependent alcohol dehydrogenase enzyme converting acetaldehyde to ethanol.

The invention discloses a Trichoderma viride F4 strain, and applications thereof. The Trichoderma viride F4 strain is stored at China General Microbiological Culture Collection Center on 10th November 2008, and preservation number is CGMCC No.2736. When the bacterial strain is used for producing cellulase taking pretreated lignocellulose as a carbon source and an inducer, the bacterial strain is capable of tolerating a plurality of inhibitors brought by pretreated lignocellulose, and producing enzyme with high efficiency; the secreted cellulase is comprehensive in composition, enzyme activity is high, and enzymolysis adaptability on pretreated lignocellulose substrate is high.

The present invention relates to a lipasegenic bacterium, its screening method and its industrial application. Said strain is a rhizopchin obtained by separating Chinese daqu liquor, its name is Rhizopus chinensis CCTCC M201021. Its screening method includes the following steps: taking daqu liquor sample or unstrained spirits, diluting and coating on plate, slant culture, shake-flask culture, centrifugal separation, injecting supernatant fluid into preliminary screen plate hole, selecting bacterium with larger transparent ring and / or fluorescent ring to make shake-flask fermentation and rescreening, freeze-drying thallus so as to obtian whole cell rhizopchin lipase enzyme preparation. It can be used for conversion synthesis of aromatic ester in orgnaic phase.

The invention relates to a black tea preparing method and particularly relates to a method for preparing congou black tea from anji white tea. The method sequentially comprises the following steps: picking based on a one-bud and one-leaf standard; withering; rolling; fermenting: arranging the rolled leaves to be fermented into a bamboo basket, controlling the leaf stacking thickness to be 8-10cm, uniformly spreading, and layering the leaves on a fermentation rack; fermenting by using a tea fermenting machine, controlling the air temperature at 35-40 DEG C, keeping the leaf temperature 30-32 DEG C, the relative humidity over 95% and sufficient oxygen supply, recording the fermentation time beginning from the rolling time, and controlling the fermentation time to be 5.5-6.0h. At the later period of fermentation, the temperature of a fermentation chamber is lowered and is controlled at 30-35 DEG C, so that the excessive fermentation is avoided; the moderate fermentation is carried out, and the fermented leaves are removed from the fermentation chamber, subjected to heat radiation, cooled and dried. By using the method, the anji white tea can be prepared into the congou black tea which is dark in luster, compact in strip, sweet and durable in fragrance, red bright in liquor color as well as pure, mild and fresh in taste.

The present invention relates to the immobilization of enzymes by adsorbing enzymes, a polyfunctional amine and a cross-linking agent onto a particulate porous carrier in a mixer apparatus or in a fluid bed apparatus.

The invention relates to a multi-hole foam glass carrier for a fast mass transfer biological fluidized bed and a preparation method and application thereof, which belong to sewage disposal technology. The multi-hole foam glass carrier is prepared by using waste glass as a raw material to be composited with volcanic rocks, coal ash, coal gangue, furnace clinker, iron powder, aluminum powder, binding agents, foaming agents, foam stabilizer and fluxing agents by weight, and multi-hole foam glass is obtained by stages of preheating, fast sintering, foaming, fast cooling and annealing. The preparation process of the multi-hole foam glass carrier is simple in route, easy to control and low in cost, and the prepared carrier is stable in performance, high in porosity, large in specific surface area, resisting in impact, good in mechanical performance, high in biocompatibility, favorable to efficient fixing of the microorganism, and suitable to the fast mass transfer biological fluidized bed. The multi-hole foam glass carrier solves the problem that biological immobilization carriers in the prior art are easy to abrade, small in bio-film formation amount, low in mass transfer effects and the like in the fast mass transfer biological fluidized bed. The preparation method opens up new approaches for preparing the biological carriers, and fills blank spaces in using of foam glass materials in sewage disposal.

An L-amino acid is produced by culturing a Methylophilus bacterium which can grow by using methanol as a main carbon source and has L-amino acid-producing ability, for example, a Methylophilus bacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation through introduction into cells, of a DNA coding for dihydrodipicolinate synthase that does not suffer feedback inhibition by L-lysine and a DNA coding for aspartokinase that does not suffer feedback inhibition by L-lysine, or a Methylophilus bacterium made to be casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.

The invention relates to a quick biological drying method for household garbage. The method comprises the following steps of: uniformly mixing the household garbage from which large impurities are screened out, a biological drying product of the garbage and broken straw, and stacking the mixture in a groove-type compost reactor; covering bundled straw cushions on the garbage surface; supplying enough oxygen to aerobic microorganisms by a timed ventilation method; and after the temperature of the stack reaches a high-temperature section (more than 55 DEG C), adopting a stack temperature feedback ventilation method to evaporate moisture by fully using heat generated by metabolism of the aerobic microorganisms, and bringing out steam by using sufficient ventilation flow to realize quick drying of the garbage. The dried garbage is separated and recycled or transported to an incineration plant for energy recovery. The method improves a lower heat value of the dried garbage by 100 percent and a separation rate by 140 percent, obviously improves household garbage incineration and separation recycling values, and can be widely applied to drying treatment of household garbage which contains 65 to 50 percent of moisture and in which biodegradable organisms account for 50 to 35 weight percent of dry matters.

The invention relates to decontaminating composites, and methods, compositions, and kits comprising the same. In some aspects, the invention relates to a decontaminating composite, comprising a perhydrolase associated with a carbon nanotube, that is useful for producing peracids.