51results about How to "Increase chlorophyll content" patented technology

The invention belongs to the technical field of soil conservation, and particularly relates to a method for promoting plant growth and regulating soil nutrients. The invention discloses a method for regulating and controlling corn and tobacco growth and rhizosphere soil nutrients through combination of AMF and a planting mode. The method comprises the following steps: preparing an AMF-containing microbial inoculum by using M. mosseae (F. mosseae); cultivating flue-cured tobacco seedlings and corn seedlings; flue-cured tobacco seedlings and corn seedlings are jointly planted in an intercropping mode by utilizing an AMF-containing fungicide. The method can relieve continuous cropping obstacles of flue-cured tobaccos and promote growth of the flue-cured tobaccos and corn, and has a certain effect on soil conservation.

The invention discloses a compound plant stress-resistant agent as well as a preparation method and application thereof. The compound plant stress-resistant agent comprises a compound of glycine betaine and polyglutamic acid, wherein the mass ratio of the glycine betaine to the polyglutamic acid is 1: (0.1-2). The preparation method of the composite plant stress-resistant agent comprises the following steps: mixing polyglutamic acid or a solution thereof with glycine betaine in ethanol to obtain a mixed solution, adjusting the pH value of the mixed solution to 3.0-6.0, carrying out an ultrasonic reaction to obtain a reaction solution, adding absolute ethyl alcohol into the reaction solution, stirring, filtering, and drying the precipitate to obtain the composite plant stress-resistant agent. The compound plant stress-resistant agent can be applied to crops in a foliage spraying or root irrigation mode, the growth conditions of grain crops such as corn, peanuts, celery and grapes and / or economic crops under adversity stress such as drought, low temperature and high salt are remarkably relieved, and the crop yield is increased.

The invention discloses a microbial agent for promoting cucumber growth and a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The microbial agent is prepared from the following components: achromobacter denitrificans, bradyrhizobium angularis and Vilnelandan nitrogen-fixing bacteria in a volume ratio of 2 to 1 to 1. Bacteria with a growth promoting effect are reasonably combined for use, so that the growth promoting effect of the mixed flora on cucumbers is better than that of a single strain, and the adaptability of the synergistic effect of the strains in the actual soil rhizosphere complex environment is higher. By determining the number of strains and mixing according to a specific proportion, germination of cucumber seeds is stably and effectively promoted, root elongation is promoted, the flowering and fruit setting rate is increased, and meanwhile the plant height, plant biomass and chlorophyll content of cucumbers in the seedling stage are increased. Besides, the microbial agent disclosed by the invention is beneficial to improving the growth quality and yield of plants and reducing environmental pollution, has wide application potential in the fields of agriculture and ecology, and is worthy of popularization.

The invention discloses application of N-acetyl-L-cysteine in improving drought tolerance of plants, and belongs to the technical field of plant cultivation. The invention provides the application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in the improvement of the drought tolerance of the brassica napus for the first time. Experiments prove that compared with a control group, the fresh weight, the dry weight, the root length and the chlorophyll content of rape seedlings subjected to foliage spraying of 150 mu M of N-acetyl-L-cysteine under the drought stress condition are improved, the water loss rate of leaves is reduced, and the drought stress tolerance of the rape is enhanced. Therefore, the drought stress tolerance of the rape treated by the N-acetyl-L-cysteine is obviously improved. Furthermore, an optimal application concentration of 150 mu M is obtained through experimental exploration, so that the N-acetyl-L-cysteine can be optimally utilized in improving the drought stress tolerance of the oilseed rape.

The invention belongs to the technical field of agriculture, and relates to an Ectoin-containing salt-alkali-resistant, high-temperature-resistant and growth-promoting composition as well as a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 12 to 15 parts of aloin, 6 to 10 parts of Ectoin, 10 to 15 parts of seaweed extract, 10 to 15 parts of gamma-aminobutyric acid, 3 to 4 parts of polyvinyl alcohol, 2 to 3 parts of sodium dodecyl benzene sulfonate, 3 to 4 parts of tween-20, 5 to 8 parts of glyceryl alcohol, 5 to 8 parts of ethyl salicylate, 5 to 10 parts of chitosan oligosaccharide and 45 to 50 parts of water. The composition disclosed by the invention can be used for remarkably improving the stress resistance of crops, relieving the membrane damage of saline-alkali and high-temperature stress to the crops, enhancing the activity of antioxidant enzymes in crop leaves under the saline-alkali and high-temperature stress and promoting the growth of the crops under the saline-alkali and high-temperature stress conditions.

The invention discloses a plant growth-promoting rhizobacteria and application thereof, and relates to the technical field of agricultural microorganisms. The strain is named as serratia sp. SYPP26, the strain is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO: 64911, and the preservation date is July 24, 2024, and the strain is named as serratia sp. SYPP26. The strain is named as serratia sp. SYPP26 and is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO: 64911. The strain is separated from the rhizosphere soil environment of the high-yield rubber tree, is high in environmental adaptability, keeps good growth consistency when the salt concentration is 1-7%, the temperature is 20-30 DEG C and the pH is 3.5-9.0, and can adapt to most of acid soil in the southern China; the strain has the capabilities of dissolving phosphorus, producing siderophores, producing indoleacetic acid, producing gibberellin, producing ammonia and the like, and experiments prove that the strain has the capability of improving the content of ammonium nitrogen and nitrate nitrogen in soil, and can improve the germination rate of rice seeds and remarkably promote plant growth and root development.

The invention discloses an irrigation method for improving the water and fertilizer utilization rate of greenhouse tomatoes, and the method adopts a method of combining a circulating aeration device with drip irrigation to control the fertilization amount, the soil water content and the irrigation amount in each period of tomato growth so as to monitor the change response of the soil environment and the migration, transformation and regulation of nutrients in a tomato root zone. By adopting an irrigation fertilization mode with a high aeration irrigation medium fertilizer level, growth of tomato plants and dry matter accumulation are promoted, root morphology distribution is improved, root activity is improved, photosynthetic indexes and chlorophyll content of the tomato plants are remarkably improved, photosynthesis is enhanced, and fruit yield, transverse diameter, longitudinal diameter and fruit quality are remarkably improved; the soil enzyme activity and the microorganism quantity of the tomato root zone are obviously improved, and the nutrient absorption accumulation amount and the nutrient absorption and utilization efficiency of tomato plants are improved.

The invention belongs to the field of soil improvement, and particularly relates to composite rice husk charcoal for improving soil and a carbon sequestration and sequestration method, rice husks are used for preparing rice husk charcoal, then the rice husk charcoal is mixed with calcium bicarbonate, and then calcination is performed to obtain calcified rice husk charcoal; and carrying out alkali treatment on the calcified rice husk charcoal to obtain the carbon sequestration and sink increase composite rice husk charcoal. The composite rice husk charcoal can effectively reduce the salt content and density of soil, increase the content of organic matters, effectively improve salinized soil, improve the survival rate and growth vigor of forest trees and promote the growth of young forests.

The invention provides a microalgae nutrient solution and application thereof, and belongs to the technical field of agriculture. The invention provides a microalgae nutrient solution. The microalgae nutrient solution comprises nitrogen-fixing blue-green algae and photosynthetic nutrient green algae, the content of algae single cells in the microalgae nutrient solution is greater than or equal to 1.0 * 10 < 6 >. The microalgae nutrient solution provided by the invention, as a microalgae fertilizer, can significantly improve the activities of urease, sucrase and catalase in soil and reduce the activity of polyphenol oxidase in the soil; the content of alkali-hydrolyzable nitrogen in soil is increased, and the nitrogen fertilizer absorption amount of beet is improved; the richness of soil microbial communities can be increased, and the number of actinomycetes and proteobacteria is increased; growth and development of the beet can be remarkably promoted, the plant height, fresh weight, leaf number, chlorophyll content and net photosynthetic rate of the beet can be improved, accumulation of dry matter of beet tuberous roots can be promoted, timely transfer of beet growth centers can be regulated, and then the yield and sugar yield of the beet can be increased.

The invention provides bacillus stutzeri HMB39561 and application thereof, and belongs to the technical field of agricultural microorganisms. The bacillus stutzeri HMB39561 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus stutzeri HMB39561 is CGMCC No.37015. The strain not only has a good prevention and treatment effect on cotton damping-off and verticillium wilt, but also can increase the cotton yield and improve the cotton quality. The bacillus stutzeri HMB39561 disclosed by the invention can be used for green prevention and control of cotton damping-off and verticillium wilt, and has a relatively good application prospect in the field of cotton planting.

The invention discloses preparation of p-methyl peptide and application of the p-methyl peptide in agriculture, and relates to the field of preparation of p-methyl peptide and application of the p-methyl peptide in plants, and the preparation method comprises the following steps: step 1, feeding and device building: adding a preset amount of L-alanine and ethylene glycol into a 250mL single-mouth round-bottom flask. As a natural cyclic dipeptide, the p-methyl peptide shows an excellent environment-friendly characteristic in plant stress resistance application. Compared with a traditional method depending on chemical fertilizers and pesticides, the method has the advantages that the method is non-toxic and harmless, soil hardening and water eutrophication caused by excessive use of the chemical fertilizers are avoided, quality safety of agricultural products is not affected as pesticide residues exceed the standard, and damage to non-target organisms and damage to ecological balance are avoided; the problem of environmental pollution caused by a traditional method is avoided from the source, and powerful support is provided for agricultural ecological environmental protection.

The invention belongs to the field of botany, and provides a method for improving the salt tolerance of ice leaf daylily seedlings, determines the optimum NaCl concentration for the growth of ice leaf daylily, and while the appropriate amount of NaCl treatment increases the yield of ice leaf daylily seedlings, it also increases the content of various nutrients (protein, soluble sugar, organic acid, dietary fiber and various amino acids) of the seedlings, and at this time, the betalain content is also the highest. Under 0mM (control NaCl concentration), 100mM (optimal NaCl concentration) and 200mM (stress NaCl concentration) NaCl treatments, the optimum nitrogen (7.5mM), phosphorus (1mM), potassium (4mM) and calcium (5mM) concentrations for the growth of ice leaf daylily seedlings are respectively determined. Under the treatments of 0mM, 100mM and 200mMNaCl, a four-factor three-level orthogonal experiment was used to determine the reasonable fertilization scheme for ice leaf helianthus seedlings under salt treatment conditions, that is, nutrient solutions with nitrogen, phosphorus, potassium and calcium of 7.5mM, 1mM, 8mM, 2mM or 10mM, 0.5mM, 8mM, 5mM respectively can significantly promote the growth of ice leaf helianthus.

The invention relates to a cultivation method of cold-resistant high-chlorophyll non-heading Chinese cabbages. The breeding method disclosed by the invention has the characteristics of simple steps, clear target and accurate breeding, a new variety of the non-heading Chinese cabbage with strong cold resistance and high chlorophyll is successfully cultivated by utilizing the breeding method, and the problem that the quality is poor due to insufficient cold resistance and low chlorophyll content of the non-heading Chinese cabbage cultivated in autumn and winter in the middle and lower reaches of Yangtze River is solved.

The application discloses a metal chelate modified lignin fertilizer additive for promoting plant growth and increasing chlorophyll content. The material is prepared by taking lignin and urea as raw materials, preparing urea modified lignin through a Mannich reaction, and then performing chelation reaction on the urea modified lignin and metal ion salt. The material can slowly release nitrogen, stabilize chelated trace elements, adjust the plant growth environment and the trace elements in the plant body, significantly increase the chlorophyll content of plant leaves, enhance the photosynthetic efficiency, and promote plant growth and biomass accumulation. The application has the advantages of simple process, easily available raw materials, environmental friendliness, natural degradation, high-value utilization of waste lignin, and provides a new scheme for efficient plant growth promoting materials, and is suitable for popularization in the field of agricultural planting.

The invention discloses a rhizosphere growth-promoting bacterium of sheep grass and its application, belonging to the field of microbial technology. The strain is classified and named as Phyllobacterium myrsinacearum, strain number B68, and deposit number CGMCC No.31906. The strain of the present invention is separated from the rhizosphere soil of sheep grass in an overgrazing sample, and is obtained by artificial separation and purification. The strain has high yield of 3-indoleacetic acid, has strong solubility for insoluble phosphates, and has good growth-promoting properties such as nitrogen fixation, ACC deaminase production, and IAA production. The prepared strain fermentation liquid is inoculated into sheep grass seedlings, and the strain can be colonized in the rhizosphere of sheep grass, regulate the bacterial community structure of rhizosphere soil of sheep grass; significantly promote the growth of sheep grass, enhance photosynthesis, and increase chlorophyll content; significantly increase the hormone and nutrient content in plant leaves and roots; significantly increase the content of available phosphorus, total carbon and organic matter in rhizosphere soil. Therefore, the myrsinacearum leaf bacillus of the present invention can be used for the development and related research of subsequent microbial fertilizers for grazing grasslands.

The invention relates to the technical field of tea production and processing, in particular to a green-keeping, bitter-reducing and freshness-increasing matcha production method which comprises the following steps: selecting a tea tree variety for planting, performing compensation fertilization, and shading and covering in the growth process to obtain fresh tea leaves; the collected fresh tea leaves are sequentially subjected to steam fixation, cooling and scattering, segmented baking, stem and leaf separation, drying, aging and alcoholization, impurity separation, low-temperature grinding and refining, and then the green-keeping, bitter-reducing and freshness-increasing matcha is prepared. Wherein the step of shading and covering is that overhead type shading and covering are carried out when one bud and two leaves grow on a tea tree, gray and blue polyethylene fiber nets are selected, the shading rate is 90%-95%, and fresh tea leaves are picked in two weeks to three weeks from the beginning of shading and covering; wherein in the low-temperature grinding, graphite is adopted for grinding and refining at the indoor temperature of 20 DEG C or below and the relative humidity of 50% or below; the method disclosed by the invention can improve the contents of amino acid and chlorophyll in the matcha, reduce the content of tea polyphenol and improve the nori fragrance of the matcha.

The application discloses a plant growth regulator. By externally applying the plant growth regulator to leguminous plants, the leguminous plants can maintain high tolerance under weak light conditions such as dense planting, strip complex planting, under forest, facility greenhouse or cloudy and rainy days, and the implementation is simple, labor is saved, and the plant growth regulator can be used for mechanical operation.

The present invention discloses a kind of endophytic fungus spiral Chaetomium of Dendrobium and its application in the growth of Dendrobium. It is classified and named as spiral Chaetomium Chaetomium spirochaete, and its classification status is Ascomycetes, Ascomycetes, Chaetomaceae, Chaetomium, and the strain number is JSNLSHQH-5. It has been deposited in the General Microbiological Center of China Microbiological Culture Collection Committee, and the deposit number is CGMCC NO.18542. The deposit date is September 2, 2019. The deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Postal Code 100101. The spiral Chaetomium spirochaete strain JSNLSHQH-5 of the present invention has obvious growth-promoting effect on Dendrobium officinale seedlings, promotes its growth, and enhances its stress resistance. At the same time, it can be artificially cultured, the culture conditions are simple, and it is easy to mass produce, and it has good development and application prospects.

The invention belongs to the technical field of agriculture, and particularly relates to application of copper-doped carbon quantum dots in improvement of crop seed and seedling stage salt tolerance. Research finds that after crop seeds are treated by using the copper-doped carbon quantum dots, the germination rate, the germination index and the germination potential of the seeds under salt stress can be obviously improved; after the copper-doped carbon quantum dots are used for treatment in the crop seedling stage, the fresh weight of the overground part and the underground part of the crop can be obviously improved under salt stress. In addition, after the crops are treated by the copper-doped carbon quantum dots in the seedling stage, the chlorophyll content and the oxidation resistance of the crops can be obviously improved.

The invention provides a breeding application of a cotton GhD05G2577 gene in increasing the plant germination rate, the biomass, the seed size and the thousand seed weight. Through a transgenic technology, an arabidopsis thaliana homozygous line plant over-expressing the GhD05G2577 gene is obtained. Compared with a wild strain, the early germination rate of a transgenic plant in a 1 / 2 MS plate is obviously increased; in the seedling stage, the leaf number is increased, the leaf length and the leaf width are increased, the plant height is increased, the chlorophyll content SPAD is increased, and the fresh weight, the dry matter content and the biomass accumulation are remarkably increased; the fruit shows that the legume length and width are increased, the seed length and width are increased, and the thousand seed weight is increased, and all reach a significant level. When the gene is applied to high-yield and income-increasing breeding of plants, the germination rate of the plants can be increased, biomass accumulation of the plants can be increased, the seed size and thousand seed weight can be increased, and an alternative scheme is effectively provided for high-yield molecular breeding of the plants.

The invention relates to the technical field of poplar cutting seedling raising, in particular to a poplar ultra-short branch cutting seedling raising method which comprises the following steps: (1) selecting poplar ultra-short branches with buds with the length of 3-5cm and the diameter of 0.3-0.5 cm, soaking and then performing surface disinfection; (2) obliquely inserting the treated ultra-short branches into a special matrix at an angle of 60-70 degrees, wherein the insertion depth is 2 / 3 + / -5% of the length of the branches; (3) placing the seedling raising plate after cuttage in an environment with the temperature of 25 + / -2 DEG C and the relative humidity of 80-90%, and spraying 2-3 times every day; (4) providing illumination by adopting an LED growth lamp; (5) after 14 days of cuttage, foliage spraying is carried out; the survival rate is increased to 90% or above and is increased by 50-60% compared with that of a traditional method, and the seedling raising cost is greatly reduced; the root activity is improved by 139%, the quantity is increased, the quality is also remarkably improved, and a solid foundation is laid for later-stage growth of plants.

The invention provides a rice straw and biogas residue-based seedling raising substrate and a preparation method thereof, the substrate is composed of a basic raw material and an additive, the basic raw material comprises, by weight, 50% of decomposed rice straw, 20% of biogas residue and 30% of vermiculite, and the additive is an additive; the additive is prepared by adding 1% of ferrous sulfate, 1% of calcium superphosphate, 1% of ammonium sulfate and 0.5% of amino acid fertilizer according to the total weight of the basic raw materials. According to the seedling raising substrate, the content of alkali-hydrolyzable nitrogen is 1204 mg / kg, the content of available phosphorus is 730 mg / kg, the content of available potassium is 2841 mg / kg, the pH value is 7.19, the conductivity is 3.23 mS / cm, the total porosity is 82%, the volume weight is 0.30 g / cm < 3 >, the seedling raising substrate can be suitable for mechanical transplanting seedling raising of rice, the seedling quality is high, the seedling slowing capacity is high, the seedling raising cost is low, the resource utilization process of rice straw and biogas residues can be promoted, environmental pollution is reduced, and the economic benefit is increased. And the method also has better economic, ecological and social benefits.

The present invention relates to the technical field of plant tissue culture, and in particular to a culture medium and a culture method for reducing vitrification of plant tissue culture seedlings. The culture medium of the present invention is a basic culture medium in which graphene oxide, amino acid-containing water-soluble fertilizer A and amino acid-containing water-soluble fertilizer B are added; the amino acid-containing water-soluble fertilizer A is a medium-element amino acid-containing water-soluble fertilizer, wherein the amino acid is ≥100g / L and Ga is ≥30g / L; the amino acid-containing water-soluble fertilizer B is a trace element amino acid-containing water-soluble fertilizer, wherein the amino acid is ≥100g / L and Fe+Zn+B is ≥20g / L. The present invention reduces the vitrification rate of tissue culture seedlings and improves the proliferation index of tissue culture seedlings by adding appropriate concentrations of graphene oxide, amino acid-containing water-soluble fertilizer A and amino acid-containing water-soluble fertilizer B to the primary culture medium and the proliferation culture medium of plant tissue culture, and has the advantages of simple and convenient operation.

The present invention provides a tung oil meal, a tung oil meal water extract and an application thereof in reducing the incidence of banana wilt disease and promoting banana growth. The tung oil meal and the tung oil meal water extract of the present invention can effectively inhibit the growth of banana wilt disease No. 4 physiological subspecies (Foc 4) in the banana cultivation process, reduce the incidence of banana wilt disease in the seedling stage, and at the same time, during the growth of banana seedlings, stimulate the activity of root polyphenol oxidase (POD), superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO) and other cell protection enzymes to increase their content, thereby stimulating root growth, especially root meristem growth, and can promote leaf growth, increase chlorophyll content, increase dry matter accumulation, etc. through the growth and development of the root system, thereby achieving effective promotion of growth and development, and has broad application prospects in the industry.

This invention relates to the field of tea cultivation technology, and more particularly to a cultivation method for improving tea quality using corn syrup. It includes the following steps: (1) planting asexual tea seedlings in containers filled with cultivation substrate and cultivating them in a greenhouse; (2) applying nutrient solution every 6-8 days during cultivation; (3) continuing cultivation until the tea seedlings sprout in early spring, and then spraying them with corn syrup solution. This invention, through foliar spraying with 0.5 mL / L corn syrup, significantly promotes tea growth and photosynthetic efficiency, improves tea quality, and under drought stress conditions, CSL0.5 treatment makes the tea leaves greener and more upright, enhancing the drought resistance of the tea trees.

The present invention provides a Guizhou Trichoderma 15-28 strain and its application, which relates to the technical field of agricultural microbial control. Trichoderma guizhouense ) strain number is 15-28, and it was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on July 18, 2024, with the deposit number CGMCC No. 41467; the bacteria can effectively promote the increase of plant height, leaf and stem diameter, biomass and chlorophyll content of Yunnan peony seedlings, and induce the resistance level of Yunnan peony plants to increase. In addition, the bacteria has a strong inhibitory effect on Alternaria alternata, the main pathogenic fungus of Yunnan peony black spot disease. Therefore, the bacteria provides an excellent strain resource for Yunnan peony growth promotion and disease resistance, and has good application prospects.

The invention discloses an amide calcium magnesium fertilizer, which is prepared from the following raw materials in parts by weight: 50 to 60.6 parts of calcium magnesium concentrated solution C and 39.4 to 50 parts of urea. The amide calcium magnesium fertilizer contains a nitrogen source, calcium ions and magnesium ions required by plant growth, so that the content of chlorophyll in plants can be increased, and the plant growth can be promoted; the invention also discloses a preparation method and application of the amide calcium-magnesium fertilizer, and the preparation method comprises the following steps: S1, adding lime milk with the solid content of 40% into calcium-magnesium liquid for neutralization to obtain neutralized liquid E; s2, filtering the neutralization solution E to obtain a neutralization filter cake A and a calcium-magnesium neutralization solution B; s3, heating and concentrating the calcium-magnesium neutralization solution B, and converting the calcium-magnesium neutralization solution B from yellow transparent to white to obtain a calcium-magnesium concentrated solution C; and S4, taking 50-60.6 parts of calcium and 39.4-50 parts of urea, uniformly mixing, heating to a molten state at the temperature of 110 DEG C or below, cooling, and granulating to obtain the amide calcium magnesium fertilizer F. The amide calcium magnesium fertilizer is formed by sequentially neutralizing, concentrating and granulating a calcium magnesium liquid byproduct produced in the nitric acid method ore dressing process, so that the utilization rate of the calcium magnesium liquid is increased.

The invention discloses a method for improving the nitrogen utilization efficiency of cucumbers and promoting growth by utilizing piriformospora indica and application, and relates to the technical field of agricultural microbial technologies and plant cultivation. According to the invention, the piriformospora indica is inoculated into a cucumber cultivation system to establish a symbiotic relationship with a cucumber root system, so that the biomass, chlorophyll content and nitrogen content of the cucumber under normal nitrogen and low nitrogen conditions can be remarkably increased. The molecular mechanism of the piriformospora indica is as follows: the piriformospora indica can obviously up-regulate the expression of a plurality of nitrogen transport protein genes (CsNPF / CsNRT2) in the root system of the cucumber, and particularly negatively regulate the expression of transcription factors CsbZIP55 and CsbZIP65 under a low-nitrogen condition, so that the inhibition effect of the piriformospora indica on downstream CsNPF genes is relieved, and nitrogen absorption is promoted. The invention provides a new technical scheme and theoretical basis for developing an environment-friendly cucumber growth-promoting microbial preparation and realizing low-nitrogen efficient cultivation.

The application provides the field of agriculture, and in particular relates to the application of a formula combination containing plant extracts in plant growth regulation activity. The active components of the formula combination are active ingredient A and active ingredient B. The active ingredient A is persimmon tannin, and the active ingredient B is chitooligosaccharide. Compared with single agents, the plant growth regulation formula combination of the application has more remarkable effects, can enhance the stress resistance of crops, promote crop growth, increase crop yield, improve the fruit quality of crops, and can be used as a detasseling agent.

The invention discloses a BnaPE1 protein and application of a coding gene of the BnaPE1 protein in regulating and controlling photosynthesis and / or leaf shape of brassica napus. The amino acid sequence of the BnaPE1 protein is shown as SEQ ID NO.2, and the nucleotide sequence of a coding region of the BnaPE1 gene is shown as SEQ ID NO.1 in a sequence table. According to the invention, a DNA fragment containing the BnaPE1 gene is separated and applied, and the fragment endows the brassica napus with the capabilities of enhancing photosynthesis and increasing and deepening leaf cracks, and has important application value for culturing a new variety of brassica napus with improved photosynthetic efficiency and yield.