16results about How to "Increase chlorophyll content" patented technology

The invention provides bacillus stutzeri HMB39561 and application thereof, and belongs to the technical field of agricultural microorganisms. The bacillus stutzeri HMB39561 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus stutzeri HMB39561 is CGMCC No.37015. The strain not only has a good prevention and treatment effect on cotton damping-off and verticillium wilt, but also can increase the cotton yield and improve the cotton quality. The bacillus stutzeri HMB39561 disclosed by the invention can be used for green prevention and control of cotton damping-off and verticillium wilt, and has a relatively good application prospect in the field of cotton planting.

The invention discloses preparation of p-methyl peptide and application of the p-methyl peptide in agriculture, and relates to the field of preparation of p-methyl peptide and application of the p-methyl peptide in plants, and the preparation method comprises the following steps: step 1, feeding and device building: adding a preset amount of L-alanine and ethylene glycol into a 250mL single-mouth round-bottom flask. As a natural cyclic dipeptide, the p-methyl peptide shows an excellent environment-friendly characteristic in plant stress resistance application. Compared with a traditional method depending on chemical fertilizers and pesticides, the method has the advantages that the method is non-toxic and harmless, soil hardening and water eutrophication caused by excessive use of the chemical fertilizers are avoided, quality safety of agricultural products is not affected as pesticide residues exceed the standard, and damage to non-target organisms and damage to ecological balance are avoided; the problem of environmental pollution caused by a traditional method is avoided from the source, and powerful support is provided for agricultural ecological environmental protection.

The invention relates to a cultivation method of cold-resistant high-chlorophyll non-heading Chinese cabbages. The breeding method disclosed by the invention has the characteristics of simple steps, clear target and accurate breeding, a new variety of the non-heading Chinese cabbage with strong cold resistance and high chlorophyll is successfully cultivated by utilizing the breeding method, and the problem that the quality is poor due to insufficient cold resistance and low chlorophyll content of the non-heading Chinese cabbage cultivated in autumn and winter in the middle and lower reaches of Yangtze River is solved.

The application discloses a metal chelate modified lignin fertilizer additive for promoting plant growth and increasing chlorophyll content. The material is prepared by taking lignin and urea as raw materials, preparing urea modified lignin through a Mannich reaction, and then performing chelation reaction on the urea modified lignin and metal ion salt. The material can slowly release nitrogen, stabilize chelated trace elements, adjust the plant growth environment and the trace elements in the plant body, significantly increase the chlorophyll content of plant leaves, enhance the photosynthetic efficiency, and promote plant growth and biomass accumulation. The application has the advantages of simple process, easily available raw materials, environmental friendliness, natural degradation, high-value utilization of waste lignin, and provides a new scheme for efficient plant growth promoting materials, and is suitable for popularization in the field of agricultural planting.

The application discloses a plant growth regulator. By externally applying the plant growth regulator to leguminous plants, the leguminous plants can maintain high tolerance under weak light conditions such as dense planting, strip complex planting, under forest, facility greenhouse or cloudy and rainy days, and the implementation is simple, labor is saved, and the plant growth regulator can be used for mechanical operation.

The invention provides a breeding application of a cotton GhD05G2577 gene in increasing the plant germination rate, the biomass, the seed size and the thousand seed weight. Through a transgenic technology, an arabidopsis thaliana homozygous line plant over-expressing the GhD05G2577 gene is obtained. Compared with a wild strain, the early germination rate of a transgenic plant in a 1 / 2 MS plate is obviously increased; in the seedling stage, the leaf number is increased, the leaf length and the leaf width are increased, the plant height is increased, the chlorophyll content SPAD is increased, and the fresh weight, the dry matter content and the biomass accumulation are remarkably increased; the fruit shows that the legume length and width are increased, the seed length and width are increased, and the thousand seed weight is increased, and all reach a significant level. When the gene is applied to high-yield and income-increasing breeding of plants, the germination rate of the plants can be increased, biomass accumulation of the plants can be increased, the seed size and thousand seed weight can be increased, and an alternative scheme is effectively provided for high-yield molecular breeding of the plants.

This invention relates to the field of tea cultivation technology, and more particularly to a cultivation method for improving tea quality using corn syrup. It includes the following steps: (1) planting asexual tea seedlings in containers filled with cultivation substrate and cultivating them in a greenhouse; (2) applying nutrient solution every 6-8 days during cultivation; (3) continuing cultivation until the tea seedlings sprout in early spring, and then spraying them with corn syrup solution. This invention, through foliar spraying with 0.5 mL / L corn syrup, significantly promotes tea growth and photosynthetic efficiency, improves tea quality, and under drought stress conditions, CSL0.5 treatment makes the tea leaves greener and more upright, enhancing the drought resistance of the tea trees.

The invention discloses a method for improving the nitrogen utilization efficiency of cucumbers and promoting growth by utilizing piriformospora indica and application, and relates to the technical field of agricultural microbial technologies and plant cultivation. According to the invention, the piriformospora indica is inoculated into a cucumber cultivation system to establish a symbiotic relationship with a cucumber root system, so that the biomass, chlorophyll content and nitrogen content of the cucumber under normal nitrogen and low nitrogen conditions can be remarkably increased. The molecular mechanism of the piriformospora indica is as follows: the piriformospora indica can obviously up-regulate the expression of a plurality of nitrogen transport protein genes (CsNPF / CsNRT2) in the root system of the cucumber, and particularly negatively regulate the expression of transcription factors CsbZIP55 and CsbZIP65 under a low-nitrogen condition, so that the inhibition effect of the piriformospora indica on downstream CsNPF genes is relieved, and nitrogen absorption is promoted. The invention provides a new technical scheme and theoretical basis for developing an environment-friendly cucumber growth-promoting microbial preparation and realizing low-nitrogen efficient cultivation.

The application provides the field of agriculture, and in particular relates to the application of a formula combination containing plant extracts in plant growth regulation activity. The active components of the formula combination are active ingredient A and active ingredient B. The active ingredient A is persimmon tannin, and the active ingredient B is chitooligosaccharide. Compared with single agents, the plant growth regulation formula combination of the application has more remarkable effects, can enhance the stress resistance of crops, promote crop growth, increase crop yield, improve the fruit quality of crops, and can be used as a detasseling agent.

The application belongs to the technical field of genetic engineering, and particularly relates to a CtPYL6 protein related to plant drought resistance, a gene and application thereof. The application provides a CtPYL6 protein related to plant drought resistance, wherein the amino acid sequence of the CtPYL6 protein is shown as SEQ ID NO. 1. The CtPYL6 protein provided by the application can improve the drought resistance of plants. The results of examples show that overexpression of the gene can improve the seed germination rate, the chlorophyll content of plants and the photosynthetic efficiency of plants. CtPYL6 Compared with wild type plants, overexpression of the gene can reduce the accumulation of malondialdehyde, superoxide anion and hydrogen peroxide, and significantly improve the activities of superoxide dismutase, catalase and peroxidase, thereby improving the drought resistance of plants. CtPYL6 The CtPYL6 protein provided by the application can obtain new germplasm with the drought resistance characteristics, and is of great significance for carrying out drought-resistant crop breeding.

The invention relates to the field of agricultural biology, and particularly discloses a preparation method of phenylalanine (cyclo (leucine-phenylalanine) dipeptide) and application of the phenylalanine (cyclo (L-leucine-L-phenylalanine) dipeptide) in agriculture. The active ingredient of the composition comprises cyclo (L-leucine-L-phenylalanine) dipeptide Cyclo (-L-Leuu-L-Phe) obtained by fermenting and extracting external myrothecium advena CGMCC No. 42202; according to the invention, the cyclic (L-leucine-L-phenylalanine) dipeptide is scientifically compounded with different bioactive substances, and an application mode of flushing or spraying is adopted, so that the growth of crops can be effectively promoted, and the stress resistance of the crops can be enhanced. Specifically, on various crops such as corn, lettuce and tomato, the composition can significantly improve key growth indexes such as plant height, root length, stem diameter and fresh weight.

This invention discloses a gene, PvHsf16, encoding a plant heat shock transcription factor, and its applications. This gene originates from differentially expressed genes upregulated in members of the Hsf gene family of switchgrass under cadmium (Cd) stress. By constructing a yeast expression vector for PvHsf16, PvHsf16 cells exhibited significantly greater cadmium tolerance, indicating that this gene enhances yeast cadmium resistance. PvHsf16 is located in the cell nucleus. Overexpression of the PvHsf16 gene in Arabidopsis thaliana, compared to the wild type under CdCl2 treatment, showed enhanced cadmium tolerance in the PvHsf6 overexpressing lines, with significantly increased chlorophyll content, root length, and biomass. The PvHsf16 gene provides a genetic resource for cultivating switchgrass germplasm suitable for both energy grass and cadmium remediation, and can be applied to the remediation of cadmium-polluted areas, playing an important role in researching phytoremediation technologies and improving soil heavy metal pollution.

The invention discloses wheat TaAOR1 protein and application of a coding gene thereof in regulation and control of plant stress resistance, and belongs to the technical field of biotechnology. The technical problem to be solved by the invention is how to improve the drought resistance of wheat. Therefore, the invention provides application of the TaAOR1 protein and related biological materials in providing wheat drought resistance, and further provides a method for regulating and controlling plant drought resistance, and the method can regulate and control the drought resistance of a receptor plant by regulating and controlling the expression quantity of a coding gene of the TaAOR1 protein in the receptor plant and / or the content of the TaAOR1 protein. An overexpression experiment proves that compared with wild type receptor wheat, the drought resistance of a transgenic wheat strain in the seedling stage and the mature stage is improved. The gene for regulating and controlling the plant stress resistance has important significance and application value for cultivating a new drought-resistant variety of the plant.

The application discloses a synergistically enhanced potassium-magnesium sulfate type compound fertilizer and a preparation method thereof, and belongs to the technical field of potassium-magnesium sulfate type compound fertilizers. The synergistically enhanced potassium-magnesium sulfate type compound fertilizer is composed of the following components in parts by weight: 200-250 parts of potassium-magnesium sulfate, 600-700 parts of powdered potassium sulfate, 40-60 parts of ammonium polyphosphate modified magnesium oxide, 10-20 parts of potassium fulvate, and 80-120 parts of a magnesium chloride-citric acid compound solution. The ammonium polyphosphate modified magnesium oxide is introduced, the unique polyphosphate long-chain structure of the ammonium polyphosphate is used as an ion lock, magnesium ions are captured and chelated in situ at a fertilizer dissolving micro-interface, and a complex with strong anti-interference capability is formed; the magnesium in this form can effectively avoid the competitive inhibition of high-concentration potassium ions on root absorption channels.

The invention provides a fungus and actinomycete complex microbial inoculant for preventing and treating root rot. The complex microbial inoculant contains spiropolyspora rosea with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.42332 and streptomyces taenanensis with the preservation number of CGMCC No.36965. The invention further provides a preparation method of the fungus and actinomycete complex microbial inoculant. The invention also provides an application of the fungus and actinomycete complex microbial inoculant. The complex microbial inoculant provided by the invention contains fermentation products of spiropolyspora roseus HLY-1 and streptomyces talanigerum NYC 1-5, the two fermentation products have no inhibition effect, and a synergistic effect can be generated by mixed application of the two fermentation products.

The invention provides stenotrophomonas bentonite KG93 and application thereof, and belongs to the technical field of agricultural microorganisms. The stenotrophomonas bentonite KG93 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.37256. The strain is separated from healthy balsam pear rhizosphere soil in a leek-balsam pear relay intercropping mode, and tests show that the bacterial colony growth inhibition rate of the strain to balsam pear fusarium wilt pathogenic fungus fusarium oxysporum balsam pear specialized type reaches 86.06%, and the prevention and treatment effects on balsam pear fusarium wilt in natural soil and sterilized soil are 73.91% and 36.05% respectively. By inoculating the strain KG93, the disease index can be obviously reduced, the chlorophyll content of bitter gourd leaves is improved, the photosynthesis is enhanced, the plant growth is promoted, and the soil enzyme activity is improved. The strain KG93 disclosed by the invention has good application potential in biological prevention and control of bitter gourd fusarium wilt.