78 results about "Salt-tolerant crops" patented technology

The invention discloses a saline-alkali soil improver and a use method thereof, and belongs to the technical field of soil improvement. The modifier comprises the following components in parts by mass: 60-80 parts of an organic carrier, 10-20 parts of an inorganic calcium material, 5-9 parts of a compound microbial agent, 10-20 parts of a sustained-release material, 5-10 parts of an acidity regulator and 15-23 parts of a plant growth promoter. The preparation method comprises the following steps: crushing the raw materials, embedding the sustained-release microspheres, loading the microbial inoculum, and granulating and drying. Through cooperation of multiple mechanisms such as calcium and sodium replacement, pH reduction by organic acid and aggregate formation promotion by microorganisms, differential application strategies are designed for light, medium and severe saline-alkali soil, salt-tolerant crop planting is matched, the yield of wheat, alfalfa and the like is increased by 18%-30%, the soil organic matter is increased to 2.1%, and the microbial diversity index is increased by 133%. The method has the advantages of long-acting salt control, ecological restoration and cost, and is suitable for treatment of coastal and inland saline-alkali soil.

The invention relates to the technical field of agricultural biology, in particular to application of a BnaC04.bZIP16 gene to improvement of salt tolerance of plants. The sequence of the BnaC04.bZIP16 gene provided by the invention is as shown in SEQ ID NO.1, and the coded protein sequence of the BnaC04.bZIP16 gene is as shown in SEQ ID NO.2. The invention discovers that the BnaC04.bZIP16 gene can be highly expressed after wild brassica napus is irrigated with saline water, and meanwhile, the transgenic brassica napus with the over-expressed gene can better grow in a salt stress environment, so that the BnaC04.bZIP16 gene can be used for preparing a salt-tolerant brassica napus. The rape with the BnaC04.bZIP16 gene knocked out is more difficult to grow in a salt stress environment, so that the effect of the BnaC04.bZIP16 gene as a forward regulatory factor in enhancing the salt tolerance of the rape is verified, and a key molecular tool is provided for developing a new variety of brassica napus with high salt tolerance; the gene has wide application potential and commercial value in the field of agricultural planting, especially in improving the salt tolerance of crops and cultivating transgenic crops with high salt tolerance.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a wheat saline-alkaline tolerant protein kinase gene TaCRCK and application thereof. Specifically, the invention discloses a wheat saline-alkaline tolerant protein kinase gene TaCRCK, and the nucleotide sequence of cDNA of the gene is as shown in SEQ ID NO. 1. The invention also discloses a plant expression vector containing the gene TaCRCK and application of the gene and the plant expression vector in cultivation of saline-alkali resistant plants. Tests prove that silent expression of the gene promotes the resistance of wheat to saline-alkaline stress, and the saline-alkaline tolerance of transgenic plants is obviously improved. The gene has a negative regulation effect on the salt and alkali tolerance of plants, provides a new tool and method for improving the salt tolerance of crops by using a genetic engineering means, and has important scientific significance and application prospects.

The invention discloses a composition containing iso-aromatic oxalic acid and taurine and application of the composition in relieving salt stress of crops. The composition is composed of iso-aromatic oxalic acid and taurine according to a mass ratio of 1: (1-4). According to the composition containing the iso-aromatic oxalic acid and the taurine, the iso-aromatic oxalic acid and the taurine are compounded according to the specific mass ratio of 1: (1-4), the growth and salt tolerance of crops can be enhanced through the synergistic effect of the iso-aromatic oxalic acid and the taurine, and especially for common food crops such as wheat and rice, the growth and salt tolerance of the crops can be improved. A good growth state can still be kept in a saline-alkali environment, the germination rate of crops is increased, tender leaves and roots are long, and the application prospect in agricultural production is very wide.

The invention discloses a saline-alkali soil salt-tolerant crop intelligent breeding evaluation method and system. The method comprises the steps of crop data acquisition, crop data improvement, crop breeding evaluation model establishment and crop intelligent breeding evaluation. The invention belongs to the field of breeding evaluation, and particularly relates to an intelligent breeding evaluation method and system for saline-alkali soil salt-tolerant crops. According to the scheme, environmental suitability grade difference regulation is introduced, and it is avoided that only a single salt-tolerant grade sample is generated; target function optimization is carried out by introducing a stress level difference coefficient, and a function is adjusted through a phenotypic sample generator feature, so that a generated sample is highly matched with an actual salt stress rule of the saline-alkali soil; evaluating contributions of different hierarchical features of the crop breeding data according to a feature importance function through a hierarchical attention mechanism, and eliminating redundant features; by introducing environmental edge loss, the distinction degree of adjacent salt tolerance grades in the extreme saline-alkaline environment is improved; the distance between adjacent salt tolerance grade characteristic centers is increased, so that the crop breeding evaluation effect is improved.

The invention discloses an RNA (Ribonucleic Acid) aptamer and application thereof in improving the salt tolerance of rice. The aptamer sequence eApt-2 with high affinity to eIF4A is obtained through enrichment by using a phylogenetic screening technology of the nucleic acid aptamer, and the aptamer sequence eApt-2 can inhibit the RNA helicase activity of eIF4A and selectively block a 5'cap-dependent translation initial process. After the eApt-2 coding sequence is introduced into a rice cell, the transgenic rice shows significantly enhanced salt tolerance under a salt stress condition: the growth condition is improved, and the biomass and the photosynthetic efficiency are both higher than those of a control plant. Compared with a traditional strategy of improving the salt tolerance through gene overexpression or knockout, the method does not need permanent modification of animal and plant genomes, accurate and reversible regulation and control of translation initiation factors are carried out through aptamers, optimization of stress response protein synthesis is achieved, and higher specificity and safety are achieved. The invention provides a novel and efficient technical approach for cultivating salt-tolerant crops, and has a wide application prospect.

The invention relates to the technical field of soil treatment, in particular to an integrated saline grainfield soil improvement and remediation method. According to the method, precise irrigation, concealed pipe drainage, salt-tolerant crop planting and bioremediation technologies are comprehensively applied, and systematic treatment of the salinized soil is achieved. Precise irrigation dynamically regulates and controls the leaching depth and frequency on the basis of real-time soil salinity and moisture data, the underground water level is intelligently controlled in combination with an underground pipe drainage network, salt accumulation is effectively reduced, and water resource waste is avoided; according to the salt-tolerant crop and green manure rotation mode, salt damage is reduced through plant salt absorption and root system nitrogen fixation, soil organic matter accumulation and microbial community reconstruction are promoted through application of a microbial agent and biochar, and a sustainable soil improvement mechanism is formed. The intelligent monitoring system integrates multiple types of sensors, collects key indexes such as salinity, nutrients and pH values in real time, predicts the salinization trend and regulates and controls irrigation, fertilization and drainage equipment in a linkage mode, full-process automatic management is achieved, and the management precision and efficiency are remarkably improved.

The invention discloses a compound microbial agent and application thereof to improvement of saline-alkali stress resistance of crops. The compound microbial agent is prepared from the following components in parts by weight: 1 to 3 parts of bacillus cereus, 1 to 3 parts of bacillus megatherium, 6 to 8 parts of rhizopus heterotropoides, 50 to 70 parts of gypsum powder, 10 to 30 parts of humic acid, 2 to 3 parts of yeast metabolite, 2 to 5 parts of aluminum sulfate, 2 to 5 parts of citric acid and 0.4 to 0.6 part of an auxiliary agent. The bacillus cereus, the bacillus megatherium and the rhizopus heterosporioides are combined for the first time, the biological functions of the bacillus cereus, the bacillus megatherium and the rhizopus heterosporioides are synergistically exerted, the growth performance of crops under saline-alkali stress is remarkably improved, the physicochemical properties and nutrient conditions of saline-alkali soil are effectively improved by adding humic acid, yeast metabolite and other components, the soil fertility is improved, and the saline-alkali soil quality is improved. Meanwhile, a favorable environment is provided for the growth of the microbial agent; the plant root activity is remarkably improved, the salt tolerance of crops under the salt and alkali stress condition is improved, the growth state of the plants is improved, and therefore the yield of the crops is increased.

The invention discloses a brassica napus Bna.A08. SNAT2 protein and application of a coding gene of the brassica napus Bna.A08. SNAT2 protein in salt stress resistance, the Bna.A08. SNAT2 gene is introduced into brassica napus through a genetic engineering technology, and it is found that the tolerance of the brassica napus in a salt stress environment can be remarkably improved through expression of the gene. Bna.A08. SNAT2 protein is used as a key salt-tolerant factor, cells are protected from being damaged by salt stress by adjusting osmotic pressure and ion balance in oilseed rape cells, germination of oilseed rape under salt stress is promoted, cultivation and popularization of salt-tolerant crop varieties are expected to be promoted through further research and application, and the application prospect is wide. Powerful support is provided for solving the problems of saline-alkali soil utilization and grain safety.

This invention relates to the field of agricultural biotechnology, specifically to... BnaC04.bZIP16 Application of genes in improving plant salt tolerance. This invention provides... BnaC04.bZIP16 The gene, whose sequence is shown in SEQ ID NO.1, and whose encoded protein sequence is shown in SEQ ID NO.2, is found in this invention to be highly expressed in wild-type Brassica napus after irrigation with salt water. BnaC04.bZIP16 Genes, and transgenic rapeseed overexpressing this gene, can grow better under salt stress conditions; knockout gene... BnaC04.bZIP16 The genetically modified rapeseed is more difficult to grow in salt-stressed environments, thus verifying... BnaC04.bZIP16 This gene plays a positive regulatory role in enhancing the salt tolerance of rapeseed and provides a key molecular tool for developing new rapeseed varieties with high salt tolerance. This gene has broad application potential and commercial value in the field of agricultural planting, especially in improving the salt tolerance of crops and breeding highly salt-tolerant transgenic crops.

The invention relates to the technical field of saline-alkali soil treatment, in particular to a severe saline-alkali soil reserve cultivated land ecological improvement method. Comprising the following steps: taking corresponding physical desalting measures on soil according to the condition of the saline-alkali soil to be improved; a halophyte community is constructed, and the soil is improved; a salt-tolerant plant community is constructed, and the soil is improved; salt-tolerant crops are planted, and soil conservation is achieved. The problems of high salinity and poor texture of saline-alkali soil can be gradually improved, and the soil quality of severe saline-alkali soil is improved.

The application provides preparation of tobacco stalk hydrolysis fermentation and application thereof in crop salt resistance, and relates to the technical field of agriculture.The tobacco stalk hydrolysis fermentation of the application is obtained by microwave-assisted hydrolysis of tobacco stalk to obtain a hydrolysis product, then the hydrolysis product is subjected to fermentation treatment by using bacillus amyloliquefaciens Cas02 to obtain a fermentation crude extract, and after freeze-drying, ethyl acetate extraction is carried out, and the residual solid phase insoluble in ethyl acetate is the tobacco stalk hydrolysis fermentation. The hydrolysis fermentation can improve the salt tolerance of crops, and can effectively alleviate the inhibition of saline-alkali stress on the growth of crops.

The invention discloses a fertilizer for improving salt tolerance of crops and a preparation method thereof, and belongs to the technical field of fertilizers. The fertilizer for improving the salt tolerance of the crops comprises the following components in percentage by weight: 50%-60% of trace elements, 5%-10% of amino acid oligosaccharin, 10%-15% of methionine, 5%-7% of halophilic strains, 5%-10% of salt-tolerant strains, 15%-20% of biochar, biochar-coated halophilic strains and biochar-coated salt-tolerant strains. According to the fertilizer for improving the salt tolerance of the crops, the halophilic strains, the salt-tolerant strains and the microelements are combined for use for the first time, and the influence on the growth of the halophilic strains and the salt-tolerant strains due to the large amount of the microelements in the combination process is prevented.

The invention relates to the technical field of plant genetic engineering, and discloses a limonium bicolor gene LbYAB1 and application thereof. The new function of the LbYAB1 is found, firstly, the expression of the LbYAB1 in salt glands and leaf primordia is proved through RNA in-situ hybridization and a promoter driven GUS tissue staining technology, and the expression of the LbYAB1 is induced by treatment of 6-benzylaminopurine. Secondly, a genetic transformation technology is updated, a stable overexpressed LbYAB1 strain is obtained, and by exploring the LbYAB1 function of an overexpressed and silent strain, it is found that compared with a wild type, development of salt glands of the overexpressed strain is inhibited, the salt secreting capacity is weakened, and the salt tolerance is reduced; a silent strain shows an opposite phenotype, and a result proves that the LbYAB1 is a negative regulation factor for salt gland development. Overexpression of the gene in limonium bicolor and arabidopsis thaliana both leads to enhancement of salt sensitivity. The invention not only lays a foundation for further exploring and revealing the development mechanism of salt glands, but also opens up a new path for cultivating salt-tolerant crops.

The application discloses a use of a wheat TaCIPK19-3D gene and a method for improving salt tolerance of crops. The wheat TaCIPK19-3D gene is taken as the object, and it is found that wild type (WT), a transgenic line overexpressing the TaCIPK19-3D gene and an oscipk19 gene knockout mutant plant all show obvious phenotype differences after salt stress. The transgenic line overexpressing the TaCIPK19-3D gene shows obvious salt tolerance phenotype, and the leaves of the transgenic line overexpressing the TaCIPK19-3D gene are greener and more active than those of the wild type and the mutant after salt treatment. The leaves of the oscipk19 gene knockout mutant line of a rice homologous gene are more withered than those of the wild type after salt treatment. The results show that the TaCIPK19-3D gene can positively regulate the salt tolerance of plants, and can be applied to the breeding of wheat salt-tolerant varieties through molecular breeding and other technologies.

The invention discloses a saline-alkali soil raw water double-micro-flooding root-promoting technology. According to the method, 150-1500 kg of a soil conditioner, 30-150 kg of a soil remediation agent and 450-1500 kg of a soil biological conditioning fertilizer are added into per hectare of original soil according to the actual salinity condition of farmland soil. According to the actual condition of crop cultivation, ditching, ridging or bedding, drip irrigation tape laying, irrigation and seeding are carried out; and the halophilic bacteria agent is conveyed and applied through a drip irrigation belt after being uniformly stirred. By synergistically promoting metabolism of halophilic microorganisms to remove salt and improve rhizosphere microenvironment of crops, root growth is facilitated, saline-alkaline resistance of the crops is improved, and high yield and high quality of the crops are achieved. The technology does not need soil dressing, needs to consume a large amount of fresh water resources, does not have complex treatment engineering, and saves cost investment. The saline-alkali soil is improved gradually and effectively while the yield and economic benefits are obtained freely through saline-alkali soil planting, and a breakthrough positive contribution is made to planting crops on the saline-alkali soil.

The invention discloses a crop saline-alkaline tolerance identification pool and an identification method, and relates to the technical field of crop saline-alkaline tolerance identification. The crop salt tolerance identification pool comprises a spray irrigation belt, a drip irrigation belt and an infiltrating irrigation pipe which are arranged in the identification pool, the spray irrigation belt, the drip irrigation belt and the infiltrating irrigation pipe are all connected to the same water conveying main pipeline, and the water conveying main pipeline is connected with a water source; a three-in-one soil sensor is arranged in the identification pool; a rain shelter capable of being far away from or covering the identification pool is also arranged above the identification pool in a sliding manner. According to the crop saline-alkaline tolerance identification pool and identification method provided by the invention, the defect that environmental factors are susceptible to external interference in traditional field identification can be effectively overcome, the accuracy and reliability of crop saline-alkaline tolerance identification results are remarkably improved, and the occurrence of the situation that the difference of experimental results is not remarkable is reduced; and a firmer technical support is provided for screening and cultivation of salt-tolerant crop varieties and treatment of saline-alkali soil.

This invention relates to a method for intercropping halophytes and forage crops in saline-alkali land. This method selects moderately to severely saline-alkali cultivated land or newly reclaimed saline-alkali wasteland in arid inland areas. Based on the principle of planting suitable crops for suitable sites, it constructs an intercropping pattern of halophytes + salt-tolerant forage crops by screening halophytes and salt-tolerant forage crops, achieving high-yield planting and improvement of the original soil in moderately to severely saline-alkali land. The method described in this invention has significant guiding value for improving the quality and efficiency of moderately to severely saline-alkali cultivated land and for the improvement and utilization of newly reclaimed saline-alkali wasteland.

The invention discloses a gene ZmGLN2 for regulating and controlling salt tolerance of corn and application of the gene ZmGLN2. The nucleotide sequence of cDNA of the gene ZmGLN2 is shown as SEQ ID NO: 1. The gene ZmGLN2 belongs to a GLN protein family and can regulate salt stress resistance and proline level under salt stress, so that the salt tolerance of crops is improved. According to the invention, two ZmGLN2 early termination mutations are separated, purified and identified by using corn EMS mutant library resources. The two mutations destroy the structure of the salt-tolerant gene ZmGLN2, the salt-tolerant phenotype and the physiological index of a mutant plant obtained by purification under salt stress are significantly reduced, the proline content is reduced under salt stress, which indicates that ZmGLN2 participates in crop salt stress response, and the gene can be used for improving the salt tolerance of crops.

The invention discloses a method and system for rapid restoration and suitable planting of a severe saline-alkali soil habitat, and belongs to the technical field of saline-alkali soil habitat restoration and suitable planting. The method comprises the steps that soil layer targeted diagnosis is carried out through multi-source physical detection equipment and a water-salt sensor array, and clay layer space distribution characteristics are obtained; constructing a three-dimensional layered salt elimination system according to spatial distribution characteristics of the clay layer, and completing salt elimination to obtain a plough layer desalted plot; carrying out soil clay layer crushing operation on the plough layer desalted land parcel by adopting a vibration type ultra-deep scarification machine tool, synchronously injecting a humic acid phosphogypsum composite modifier and a decomposed organic fertilizer, and carrying out rotary tillage to obtain a soil body structured land parcel; salt-tolerant crops coated with arbuscular mycorrhizal fungi are sown in the soil body reconstructed land parcels, and water and fertilizer regulation and seedling stage bacterial liquid dilution root irrigation are implemented through intelligent drip irrigation. The method realizes rapid restoration of the saline-alkali soil, has ecological and economic benefits, and is suitable for treatment of severe saline-alkali soil such as coastal saline-alkali soil.

The application discloses a kind of composite microbial inoculant and its application in improving the ability of crop to resist saline-alkali stress, the composite microbial inoculant includes the following weight fraction components: bacillus cereus 1-3 parts, bacillus megaterium 1-3 parts, hetero root sporocyst 6-8 parts, gypsum powder 50-70 parts, humic acid 10-30 parts, yeast metabolite 2-3 parts, aluminum sulfate 2-5 parts, citric acid 2-5 parts and adjuvant 0.4-0.6 parts.The application combines bacillus cereus, bacillus megaterium and hetero root sporocyst for the first time, and synergistically exerts its biological function, significantly improves the growth performance of crops under saline-alkali stress, by adding humic acid, yeast metabolite and other components, effectively improves the physical and chemical properties and nutrient status of saline-alkali soil, improves soil fertility, and provides a favorable environment for the growth of microbial inoculant;Significantly improve plant root activity, improve the salt tolerance of crops under saline-alkali stress conditions, improve the growth state of plants, thereby improving the yield of crops.

This application relates to the field of plant genetic engineering technology, specifically disclosing the application of a sterol synthesis-related gene in the response regulation of plant salt tolerance. This application clarifies the sterol synthesis-related gene. SMT2 This study expands our understanding of the role of sterol biosynthesis in plant stress response by revealing the function of calcium signaling under salt stress; it also unveils a novel mechanism by which sterol-membrane lipid interactions regulate calcium signaling, providing a new perspective for research on plant salt stress signal transduction; furthermore, this application provides new salt tolerance gene resources. SMT2 Genes can be used for molecular breeding of salt-tolerant crops, to breed salt-tolerant crop varieties, or to develop salt-tolerant molecular markers.

The invention discloses application of a plant source extract in saline-alkali soil crop stress resistance and yield increase, and belongs to the technical field of plant growth regulation. Salvia miltiorrhiza and safflower are crushed and sieved, deionized water is added, and ultrasonic-assisted extraction is adopted to obtain a water extract; and concentrating the water extracting solution, and freeze-drying to obtain extract dry powder, namely the salvia miltiorrhiza and safflower combined extract. The concentration of the salvia miltiorrhiza and safflower combined extract is 1 microgram / mL, the saline-alkali soil is light and moderate coastal saline-alkali soil, and the salt content is 2.5-4 per mill; the crop is corn. The salvia miltiorrhiza and safflower combined extract is sprayed on leaf surfaces of crops planted in the saline-alkali soil, and the spraying amount is 225-375L hm <-2 >. The salvia miltiorrhiza and safflower combined extract is sprayed on leaf surfaces once in the large horn mouth period and the tasseling period of corn respectively, so that the salt tolerance of crops can be remarkably improved, the net photosynthetic rate, the transpiration rate, the stomatal conductance, the dry weight of a single plant and the yield are improved, and the intercellular CO2 concentration of saline-alkali soil crops is reduced.

The invention belongs to the field of plant genetic engineering, and particularly relates to application of an AhSTF1 gene in regulation and control of plant salt tolerance. The base sequence of the AhSTF1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the protein expressed by the AhSTF1 gene is as shown in SEQ ID NO.2. Experimental results show that the AhSTF1 gene relieves damage caused by salt stress through synergistic effects of improving POD activity, reducing MDA content and accumulating soluble protein, and finally, phenotypes show that the germination rate is higher, the root system is more developed, and the growth condition is better. The results fully prove that the AhSTF1 gene plays a key and positive role in improving the salt tolerance of plants, provides important gene resources and theoretical basis for stress-resistant breeding of peanuts, and has important application value for cultivating new varieties of salt-tolerant crops.

The invention discloses a salt-tolerant active substance generated by a Panchondroius sp. PanST and an application of the salt-tolerant active substance. The molecular formula of the substance is C40H44O18, and the substance has the effect of improving the salt tolerance of rice, obviously increases the plant height, the root length, the overground part fresh weight and the underground part fresh weight of the rice, is stable in effect, and has a relatively good application prospect; the compound can be used as a preparation for improving the salt tolerance of crops in agricultural green production.

The invention discloses application of peanut exocrine peptide AhSIP1 in improving salt tolerance of plants, and belongs to the technical field of molecular breeding. The nucleotide sequence of the peanut exocrine peptide AhSIP1 is as shown in SEQ ID NO: 1, and the sequence of amino acid coded by the peanut exocrine peptide AhSIP1 is as shown in SEQ ID NO: 2. Experiments prove that when the AhSIP1 is mature and applied in vitro, peanut seed germination under salt stress can be remarkably promoted, 35S: AhSIP1 transgenic apple callus grows faster (fresh weight is larger) under salt stress, and it is indicated that the peanut exocrine small peptide AhSIP1 positively regulates plant salt tolerance. Functional identification of the peanut exocrine small peptide AhSIP1 provides theoretical basis and technical guidance for seedling emergence, full seedling and high-yield cultivation of saline-alkali soil plants, and has important application value in salt-tolerant crop breeding.

The application discloses a salt-tolerant crop and microorganism synergistic saline-alkali soil clearing and repairing system and method, and relates to the technical field of saline-alkali soil improvement.The application comprises the following steps: collecting soil samples of a target saline-alkali soil, performing soil physical and chemical property determination, and obtaining soil physical and chemical parameters; performing saline-alkali degree grading according to initial pH value and salt content, calculating the amount of salt-tolerant microorganisms to be put according to the saline-alkali grade and salt content, performing salt clearing treatment, and obtaining salt clearing state parameters; determining salt-tolerant microorganism putting control parameters, obtaining pH values multiple times and updating the salt-tolerant microorganism putting control parameters until the pH value reaches a preset target interval; outputting the matching parameters of organic fertilizer and original microbial flora according to the organic matter content and nutrient content, and performing soil microbial flora transplantation; collecting real-time monitoring parameters and crop growth state parameters, and adjusting the salt-tolerant microorganism putting control parameters.The application realizes multi-stage cooperative repair of saline-alkali soil based on soil state information driving.

The invention belongs to the technical field of salt-resistant planting of crops in coastal saline-alkali soil, relates to a seed coating agent with a double-layer structure for improving the salt resistance of crops in saline-alkali soil as well as a preparation method and application of the seed coating agent, and is suitable for seeding and early growth of crops such as corn and the like under a saline-alkali soil condition. According to the invention, a technical thought that seeds are suitable for places is adopted, and a stable microenvironment is formed in crop germination and early seedling stage by constructing an internal and external synergistic double-layer coating structure on the surfaces of the seeds, so that the salt tolerance of the crops is enhanced. In the double-layer structure, the inner layer is a hydrogel system composed of silk fibroin, trehalose and nano magnesium oxide and is used for relieving salt stress induced permeation unbalance and oxidative damage; the outer layer is a water-swelling gel layer formed by compounding pectin, sodium carboxymethyl cellulose and monosaccharide, and a buffer layer is formed through water swelling, so that salt ions are inhibited from migrating to the periphery of the seeds, and the environmental conditions at the initial germination stage are improved. The synergistic effect of the inner layer and the outer layer is beneficial to stable germination and growth of crops in saline-alkali soil.

The present invention discloses an RNA aptamer and its application in improving the salt tolerance of rice. The present invention utilizes the systematic evolution screening technology of nucleic acid aptamers to enrich and obtain an aptamer sequence eApt‑2 with high affinity for eIF4A, which can inhibit the RNA helicase activity of eIF4A and selectively block the 5' cap-dependent translation initiation process. After the eApt‑2 coding sequence is introduced into rice cells, the transgenic rice shows significantly enhanced salt tolerance under salt stress conditions: the growth condition is improved, and the biomass and photosynthetic efficiency are higher than those of the control plants. Compared with the traditional strategy of improving salt tolerance by gene overexpression or knockout, the method of the present invention does not require permanent modification of the plant genome, and utilizes aptamers to perform precise and reversible regulation of translation initiation factors, thereby achieving optimization of stress-responsive protein synthesis, and has higher specificity and safety. The present invention provides a novel and efficient technical approach for cultivating salt-tolerant crops, and has broad application prospects.

The invention relates to the technical field of soil remediation, in particular to a saline-alkali soil remediation fungicide and a preparation method thereof. Comprising the following raw materials in parts by weight: 4 to 8 parts of bacillus subtilis, 8 to 15 parts of purple sulfur bacteria, 10 to 17 parts of rhizobium, 1 to 3 parts of bacillus megatherium, 1 to 3 parts of candida tropicalis, 1 to 3 parts of aspergillus niger, 0.05 to 0.2 part of proline, 0.5 to 2.5 parts of humic acid, 3 to 5 parts of modified charcoal, 0.1 to 0.5 part of glucose, 0.4 to 1.2 parts of monopotassium phosphate and 0.3 to 0.6 part of calcium chloride. The application of the soil remediation fungicide not only improves the growth environment of the crops, but also obviously improves the salt tolerance of the crops by adjusting the stress resistance mechanism of the crops. After the soil remediation fungicide is applied, the root system development of crops is more robust, and the density and length of the root system are obviously increased, so that the crops can absorb water and nutrients from the soil deeper layer, and the surface salt damage can be effectively resisted.