Method for identifying quality of cotton salt-tolerant seeds

An identification method and salt-tolerant technology, applied in the agricultural field, can solve problems such as cotton damage and achieve the effect of high-throughput detection

Inactive Publication Date: 2011-09-07
JIANGSU COASTAL AREA AGRI SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil salinity below 0.2% is suitable for the emergence and growth of cotton, but when the soil salinity is greater than 0.3%, it will cause harm to cotton

Method used

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  • Method for identifying quality of cotton salt-tolerant seeds
  • Method for identifying quality of cotton salt-tolerant seeds
  • Method for identifying quality of cotton salt-tolerant seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Indoor germination experiment I-identification of double-layer filter paper

[0014] The specific method of the double-layer filter paper method is to first place a piece of filter paper soaked in salt water (0.4% sodium chloride solution) under the plastic square, then put the seeds soaked in salt water (0.4%) on the filter paper, and then cover it. Put a layer of filter paper on it, and the filter paper above is also soaked in 0.4% salt water. The test materials include salt-tolerant strains: Yan 1032, Yan 1129, and Yan 2018. Controls: Sumian No. 9 and Sikang No. 1 (other non-emerging materials are omitted). 1 row for each material, 16 grains per row, set up two replicates, divided into salt treatment and blank control (Table 2). Remove the upper layer of filter paper and soak in salt water (0.4%) every day. The cultivation environment was an artificial incubator, and the temperature was set at 30 degrees. Germinate in three days, and count the germination rate...

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Abstract

The invention discloses a method for identifying quality of cotton salt-tolerant seeds. Salt-tolerant seeds are screened by adopting an indoor experiment and an outdoor experiment, wherein the indoor experiment comprises an indoor germination experiment I and / or an indoor germination experiment II; and the outdoor experiment comprises outdoor salt-tolerance identification I and / or outdoor salt-tolerance identification II. In the invention, the salt-tolerance character of cotton is integrally identified and evaluated by adopting double-layered filter papers, sand, salt ponds and heavy alkali-salinity soils by the seaside. The salt-tolerance in the germination stage, the seedling stage and the plant-forming stage of cotton can be identified and evaluated. The salt-tolerance of cotton is detected roundly and stably with high efficiency and high throughout. The integral identification and evaluation of multi-stage, multi-place and multi-level can be performed for the salt-tolerance of crops which are controlled by complicated quantitative inheritance genes and greatly influenced by external environment.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a cotton salt-tolerant germplasm identification method. Background technique [0002] At least 20% of the cultivated land in the world is salinized. The high concentration of salt in the soil causes osmotic stress and ion poisoning to crops, which affects the growth of crops and seriously leads to crop yield reduction. There are about 40 million hm of salinized and secondary salinized land in my country 2 The above have become the main limiting factors for the development of agricultural production in our country. The coast of Jiangsu has vast saline-alkali land, and Yancheng alone has a tidal flat area of ​​453,000 hm 2 above, accounting for about 70% of the province, and the annual output is 666.7hm 2 The speed above is constantly silting outwards. Jiangsu Province put forward the "Science and Technology Development Strategy" early on, especially in 2009, "Jiangsu Coa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00G01N17/00
Inventor 王为潘宗瑾潘群斌蔡立旺陈建平汪保华王长彪陈浩东
Owner JIANGSU COASTAL AREA AGRI SCI RES INST
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