72 results about "Chitinase" patented technology

The invention discloses an antibacterial peptide fragment intercepted from chitinase IDGF4 protein and application of the antibacterial peptide fragment in preparation of antibacterial drugs. According to the invention, short peptide fragments in chitinase IDGF4 protein are screened, three candidate peptides are obtained through chemical synthesis, the antibacterial performance of the candidate peptides is systematically evaluated, and the evaluation result shows that the peptide fragment with the amino acid sequence as shown in SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg / mL; the antibacterial effect of the compound is confirmed in inhibition zone experiments, enzyme-labeled turbidimetry and growth curve monitoring, and the compound shows stable and remarkable antibacterial activity and can be used as a novel antibacterial molecule; the invention provides a new candidate sequence for developing the antibacterial peptide with high efficiency and low drug resistance risk, and has application potential in the aspect of preparing clinical antibacterial drugs.

The present application relates to "application of a compound in soybean cyst nematode chitinase inhibitor", belong to the technical field of leguminous plant symbiotic protection, the application of the compound and its pharmaceutically acceptable salt in soybean cyst nematode chitinase inhibitor, the present application is evaluated and researched through the inhibitory activity of compound by screening work, the inhibitor is screened finally, the inhibitor is likely to have good application prospect in controlling soybean cyst nematode disease aspect.The present application also provides a kind of chitinase inhibitor with the function of inhibiting the establishment of symbiosis of soybean cyst nematode to destroy, the number of nodule obtained by processing is flat with the number when only inoculating rhizobium, the inhibitory effect of Hg-CHI-1 on rhizobium nodule is completely restored, and it has good application prospect in the aspect of leguminous plant symbiotic protection.

This invention discloses a chitinase-producing Bacillus albus A01 and its application in the fermentation of shrimp and crab solid waste. The chitinase-producing Bacillus albus is named... Bacillus albus A01, with accession number CCTCC: M 20252571; the 16S rDNA gene sequence of this chitinase-producing Bacillus albus strain A01 is shown in SEQ ID NO.1. The fermentation agent of this strain can effectively degrade the chitin content in shrimp and crab solid waste, and improve the nutritional value of shrimp and crab fermented powder through solid-state fermentation. This invention solves the problem of unsatisfactory fermentation effects of existing feed fermentation strains, and has great application potential for improving the feed nutritional quality of shrimp and crab fermented powder and developing feed resources.

The present application relates to a kind of organic water-soluble fertilizer and its preparation method, which uses fish waste and shell waste as raw material, after being crushed and sterilized, enzymatic hydrolysis is carried out using acid protease and chitinase to obtain enzymatic hydrolysate;Add molasses to the enzymatic hydrolysate, and inoculate mixed bacterial agent for primary fermentation to obtain fermentation liquor A;Add yeast, neutral protease and sodium alginate to the fermentation liquor A, and carry out secondary fermentation under stirring to obtain fermentation liquor B;After treating the fermentation liquor B, add chitosan to obtain the organic water-soluble fertilizer;The organic fertilizer prepared by the method of the present application can utilize shell waste, and the obtained organic water-soluble fertilizer can improve its water-retaining and fertilizer-retaining performance in sandy soil while meeting the standard requirements of amino acid water-soluble fertilizer.

The application belongs to the technical field of agricultural fertilizer, and particularly relates to a high-efficiency yield-increasing liquid fertilizer containing enzymatic nereid and a preparation method thereof, which comprises the following steps: washing and crushing nereid, and then performing freeze-thaw cycle pretreatment; under weak alkaline conditions, a complex protease preparation containing protease and chitinase is used for enzymolysis, and an enzymatic nereid liquid is obtained through solid-liquid separation; urea, phosphate-potassium fertilizer, trace elements, potassium fulvate and polyaspartic acid are mixed in a preparation tank, and then N-carbamoyl-L-glutamic acid-chitosan graft copolymer and seleno-cysteine-alginic acid nanometer microspheres are added to obtain a nutrient liquid; finally, the enzymatic nereid liquid, the nutrient liquid and a plant growth regulator are mixed to obtain a finished product. Through the biological enzymolysis technology, the active substances in the nereid are fully released, the prepared liquid fertilizer has the functions of nutrient supply, physiological regulation and soil improvement, can significantly promote crop growth and increase yield, and realizes high-value utilization of marine biological resources.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The invention discloses a high-deacetylation-degree chitosan preparation method, which comprises: suspending chitin in a solvent to prepare a chitin suspension; adding a compound enzyme into the obtained suspension liquid to carry out enzymatic reaction, so as to prepare the chitosan with high deacetylation degree; wherein the compound enzyme is prepared from chitin deacetylase, chitinase, beta-N-acetylglucosaminidase and protease. According to the method, four enzymes with complementary functions are creatively combined to form a unique technical path of four-stage synergistic interaction of'removal-deconstruction-assistant-deacetylation ', so that a natural chitin crystal barrier can be efficiently deconstructed, the protein shielding effect is overcome, and the effect of directly preparing high-deacetylation-degree chitosan from chitin is achieved.

The application discloses a kind of low temperature resistant strain YW-1 for preventing and treating rice seedling wilt, and belongs to the field of plant protection.The low temperature resistant strain YW-1 is a pseudo-odor class fungus, and the application proves that the strain YW-1 can maintain normal growth and metabolism in a low temperature environment of 15 DEG C, and can effectively destroy the hyphal structure of the wilt pathogen by secreting cellulase and chitinase, thereby inhibiting the occurrence of the disease from the source.The strain YW-1 has no antagonism with the commonly used functional fungus Trichoderma in the seedling raising substrate, and the two can be compounded and coexist, thereby realizing the synergistic effect of functional microorganisms.The application overcomes the defects of the existing biocontrol strain, such as reduced activity in a low temperature environment and unstable prevention effect, and provides a stable and reliable biological control scheme for factory seedling raising in a low temperature season without increasing the amount of chemical pesticides, thereby significantly improving the quality of seedlings and the seedling raising success rate.

This invention discloses a method for improving the resistance to postharvest black spot disease in sweet potato tubers and maintaining their quality, belonging to the field of postharvest preservation technology for agricultural products. The method includes: selecting undamaged sweet potato tubers for callus treatment at room temperature for 48 hours, disinfecting with 1% sodium hypochlorite, rinsing with sterile water and air-drying, then soaking them in a 9.0 mmol / L benzothiadiazole (BTH) solution (containing 0.05% Tween 80) at room temperature for 60 minutes, removing them and air-drying them to complete the treatment. This invention induces the activation of the sweet potato tuber's own defense system through BTH, increasing the activity of key enzymes in phenylpropane metabolism, chitinase and other disease-resistant enzymes, promoting the accumulation of total phenols and lignin, maintaining cell membrane integrity, inhibiting starch degradation and abnormal increase in soluble sugars. Under storage conditions of 28±1℃ and 80-85%RH, it can significantly inhibit the expansion of black spot disease lesions and extend the storage period to more than 25 days. This method has no pesticide residues, is unlikely to cause pathogen resistance, is environmentally friendly, simple to operate, and cost-controllable, and is suitable for large-scale postharvest preservation of sweet potato tubers.

The invention relates to the technical field of gene engineering, and particularly discloses a chitinase truncated body, a recombinant expression vector thereof, and a construction method and application of engineering bacteria. The chitinase truncated body is Cq181NC, the amino acid sequence of the Cq181NC is as shown in SEQ ID NO: 1, and the nucleotide sequence for coding the chitinase is as shown in SEQ ID NO: 2. According to determination, the chitinase truncation provided by the invention has the advantages that the specific enzyme activity is obviously improved by 1.8 times compared with that of a wild type, the chitinase truncation is high in environmental change tolerance, and the thermal stability of the chitinase truncation is still 1.43 times that of the wild type (incubation is performed for 8 hours at 35 DEG C). More importantly, the spectrum of the enzymolysis product is changed: the wild type is mainly disaccharide and shows a typical excision enzyme mode; and the truncated Cq181NC generates a plurality of oligosaccharides including monosaccharide, disaccharide, trisaccharide and hexasaccharide, shows double catalytic characteristics of endonuclease and exonuclease, and has a better application prospect.

The invention discloses a method for producing oligosaccharide by cooperatively treating aspergillus niger residues through multiple enzymes. The method comprises the following steps: mixing pretreated aspergillus niger residues with hydrochloric acid, carrying out acidolysis wall breaking, and carrying out suction filtration on an obtained wall-broken mixture; carrying out mixed treatment on filtrate obtained by suction filtration with cellulase and beta-glucanase, filtering, and carrying out concentration and alcohol precipitation on supernate to obtain soluble beta-glucan oligosaccharide; performing filter residue enzymolysis on filter residues obtained by suction filtration by using mesoporous silica immobilized chitinase, and recovering the immobilized chitinase after enzymolysis; and filtering the mixed solution subjected to enzymolysis by using the immobilized chitinase, and carrying out alcohol precipitation on the supernate to obtain the N-acetylglucosamine and the chitosan oligosaccharide. According to the method disclosed by the invention, the aspergillus niger residues are jointly treated by adopting multiple enzymes, the yield of N-acetylglucosamine and chitosan oligosaccharide reaches 85.1%-88.5%, and the yield of beta-glucosamine oligosaccharide reaches 86.7%-89.3%. The invention provides a brand new route for producing various oligosaccharides by utilizing solid waste aspergillus niger residues in the fermentation industry.

The invention discloses a bacillus subtilis CR-6 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the bacillus subtilis CR-6 is CGMCC (China General Microbiological Culture Collection Center) No.35148. The bacillus coagulans ER-6 has the characteristic of high yield of chitinase, has a strong contact killing effect on meloidogyne incognita and reniform reniform nematodes no matter fermentation liquor or fermentation supernate, is applied to prevention and control of agricultural pests, does not involve broad-spectrum chemical toxins, does not have harmful residues, and meets the requirements of green agriculture and ecological safety. Meanwhile, the bacillus coagulans ER-6 disclosed by the invention also has a growth promoting effect on plant growth, and the yield of crops is remarkably increased.

The invention provides a high-activity exo-chitinase mutant and application of the high-activity exo-chitinase mutant in degradation of chitin waste, and belongs to the technical field of enzyme engineering and waste bioconversion. According to the present invention, the semi-rational engineering design is performed on the wild type exo-chitinase derived from Paenibacillus barengltzii, and a group of exo-chitinase mutants with characteristics of high activity, high stability and strong substrate binding affinity are constructed through the site-directed mutagenesis and domain fusion strategy; the exo-chitinase mutant shows excellent thermal stability and pH stability in a wide temperature and pH range, and the exo-chitinase mutant or an enzyme preparation containing the exo-chitinase mutant can be used for large-scale degradation of agricultural and marine chitin wastes. The method has a wide application prospect in the fields of sustainable feed production, agriculture and industrial biotechnology.

This invention discloses a truncated chitinase TSCHI-T, its preparation method, and its applications. The amino acid sequence of the truncated chitinase TSCHI-T is shown in SEQ ID NO.3. The truncated chitinase obtained by this invention has high hydrolytic activity for low-deacetylated chitosan. The crude enzyme solution produced by shake-flask fermentation has the ability to degrade 10g of low-deacetylated chitosan by 1mL, while hydrolyzing the same mass of low-deacetylated chitosan requires approximately 300mg of non-specific commercial enzyme. The enzymatic hydrolysis efficiency is increased by nearly 700 times, and it can be used for large-scale preparation of low-deacetylated chitosan oligosaccharides.

The invention discloses a method for synchronously preparing chitosan and citric acid through a kitchen waste lobster shell fermentation method, belongs to the field of solid waste resource utilization, and aims to solve the problems of difficult treatment, low utilization rate and pollution caused by impurity wrapping in shellfish kitchen waste treatment. According to the method, kitchen waste containing grease, chitin, calcium carbonate and protein is used as a raw material, and two-stage treatment mainly including fermentation is carried out. In the first stage, citric acid is produced through fermentation, and chitin is exposed through in-situ deoiling, decalcification and deproteinization; in the second stage, enzymolysis is mainly carried out, and after the pH is adjusted, chitinase and chitin deacetylase are exogenously added to convert chitin into chitosan; and finally, separating the feed liquid to obtain citric acid and chitosan. According to the method, fermentation is combined with enzymolysis segmented treatment, so that the problem of substrate wrapping is solved, the conversion rate and purity of chitosan are remarkably improved, high-valued co-production of waste is realized, and the process is mild and environment-friendly.

The invention concerns a method for prophylaxis of infections by fungi, particularly by oomycetes, and of bacterial infections in crop and ornamental plants. Areas of application are vine, fruit, vegetable and ornamental plant growing. The method of the invention is characterized in that an aqueous solution of a protease, alone or in combination with β-glucanases and / or chitinases, is prepared, stabilizers, stickers and wetters are added, and the additized solution is applied by conventional techniques to the plants a number of times within the vegetation period, preferably ahead of weather-related phases of high infection threat.

The invention belongs to the technical field of seasoning processing, and particularly relates to a method for improving the antibacterial activity of shrimp shell powder in salt-reduced soy sauce production and use processes. The method mainly comprises the following steps: decalcifying and crushing shrimp shells by comprehensively utilizing an acid solution, microwaves and a superfine crushing technology, taking the shrimp shells as part of production raw materials of the salt-reduced soy sauce, and degrading chitin in the shrimp shells into low-molecular-weight chitosan by utilizing a chitinase system and a chitosanase system produced by microorganisms in a koji-making and soy sauce mash fermentation process. The antibacterial activity of the compound in the fermentation and use processes of the salt-reduced soy sauce is improved, and the biological safety of the salt-reduced soy sauce in the production and use processes is guaranteed. Compared with the conventional soy sauce (the saline concentration is 22%, w / v), the content of NaCl in the salt-reduced soy sauce produced by the method disclosed by the invention is reduced by more than 28%, the content of organic substances (amino acid nitrogen, total nitrogen, salt-free solids and the like) is increased, and the flavor of the salt-reduced soy sauce is improved. Therefore, the method disclosed by the invention has a wide application prospect.

The invention belongs to the technical field of microorganism application, and particularly relates to bacillus pacificus YM01 capable of degrading feathers and application of the bacillus pacificus YM01. The bacillus pacificus YM01 is preserved in the China Center for Type Culture Collection on June 23, 2025, and the preservation number of the bacillus pacificus YM01 is CCTCC (China Center for Type Culture Collection) M 20251388. The strain can rapidly grow by taking feather as a unique carbon and nitrogen source and degrade and convert the feather into small molecular compounds such as peptide and amino acid, a culture method of the bacillus pacificus YM01 is optimized, conditions for degrading the feather by fermentation liquor are studied, keratinase BPK1 and chitinase BPC1 are screened out, and the application prospect is wide. The invention further establishes a method for degrading feathers by using the strain and fermentation broth thereof, namely extracellular crude enzyme, and provides a feasible technology for biotransformation and utilization of feather resources.

The application provides a blood-activating and stasis-removing medicinal composition containing fermented douchi and a preparation method thereof, belongs to the technical field of biological medicine, and the medicinal composition comprises fermented douchi, drynaria extract, extract of pereskia aculeata, tanshinone II A, notoginsenoside R1, ligustrazine, rhodioside, total flavones of hawthorn leaves and total flavones of mulberry leaves. The fermented douchi is prepared from soybeans and black beans through fermentation by bacillus subtilis and bacillus coagulans. The drynaria extract is prepared from drynaria powder through common enzymolysis by cellulase, chitinase and beta-glucanase, extraction by a two-phase solvent, and purification by a dextran gel column. The extract of pereskia aculeata is prepared from pereskia aculeata powder through common enzymolysis by pectinase, cellulase and xylanase, common enzymolysis by bromelain and subtilisin, and purification by a macroporous adsorption resin column. The medicinal components are prepared in a specific proportion to relieve blood stasis from two dimensions of "improving blood flow dynamics + preventing thrombus formation".

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas aeruginosa and application thereof. The invention discloses Pseudomonas aeruginosa R5-2, and the number of the Pseudomonas aeruginosa R5-2 is CGMCC (China General Microbiological Culture Collection Center) No.34796. The invention further discloses a preparation method of the Pseudomonas aeruginosa R5-2. The strain can secrete various active substances such as siderophores and chitinase, and can effectively inhibit growth of various pathogenic bacteria such as fusarium oxysporum, verticillium dahliae, rhizoctonia solani and fusarium graminearum. Experiments show that a fungicide containing the strain has a remarkable prevention and treatment effect on lycium barbarum root rot and tomato fusarium wilt caused by fusarium oxysporum. The invention provides a novel and environment-friendly microbial resource for preventing and treating soil-borne fungal diseases of crops such as medlar, tomato and the like, and has important application value.

The invention relates to the technical field of gene engineering, and particularly discloses exo-chitinase Cq181 and application thereof in preparation of chitobiose. The amino acid sequence of the chitinase is as shown in SEQ ID NO: 1, and the nucleotide sequence of a Cq181 gene for coding the chitinase is as shown in SEQ ID NO: 2. According to determination, the molecular mass of the chitinase is 82.53 kDa, the chitinase shows high activity and specific production of chitobiose under the conditions that the temperature is 40 DEG C and the pH value is 7.0, and the chitinase is suitable for a mild and low-energy-consumption biological manufacturing process and particularly has good application potential in the aspect of preparing chitosan oligosaccharide with a specific polymerization degree (the polymerization degree is 2).

The invention discloses pichia pastoris engineering bacteria capable of efficiently expressing chitinase and application of the pichia pastoris engineering bacteria in shrimp shell high-value processing, and belongs to the field of biological engineering. The pichia pastoris engineering bacterium takes pichia pastoris GS115 as a host, the high-purity CtChi70 enzyme preparation can be prepared by signal peptide optimization, high-copy strain screening and cofactor overexpression in combination with high-density fermentation in a fermentation tank, ultrafiltration concentration in a hollow fiber column and freeze-drying flow, and CtChi70 accounts for more than or equal to 90% of total protein in fermentation liquor. The lyophilized enzyme preparation is used for a chitin substrate extracted from shrimp shells after mild treatment, and a product mainly comprising chitobiose can be efficiently generated. According to the invention, high yield of CtChi70 and simple preparation of the preparation are realized, and an economical, environment-friendly and high-yield feasible path is provided for high-value utilization of waste shrimp shell resources.

The application discloses a preparation method and application of chitooligosaccharide alduronic acid and belongs to the technical field of marine bioengineering. The preparation method comprises the following steps: after beta-chitin and a cleaving polysaccharide monooxygenase SmLPMO10 are dissolved in a Tris-hydrochloric acid buffer solution, a reducing agent is added, and an enzymatic reaction is carried out; after the reaction is carried out for 24 hours, the reducing agent is supplemented, the reaction is continuously carried out, the reducing agent is supplemented once every 24 hours, after the reaction is carried out for 96 hours, supernatant is obtained by filtration, and the chitooligosaccharide alduronic acid is obtained after inactivation, centrifugal removal of supernatant, desalination and freeze-drying. The chitin can be cleaved by using a single cleaving polysaccharide monooxygenase, without additional addition of chitinase, and the reaction condition is mild, so that the generation of by-products and environmental pollution are avoided. The application provides an environment-friendly preparation method of chitooligosaccharide alduronic acid, and the research and development of a green conversion technology of chitin and chitin-like biomass resources are greatly promoted.

The application belongs to the technical field of microorganisms, and specifically discloses a chitinolytic Paenibacillus which can improve tea yield and tea quality and an application thereof. Paenibacillus chitinolyticus The chitinolytic Paenibacillus has a preservation number of CCTCC NO: M 20241893, has high-efficiency potassium-dissolving activity, strong organic phosphorus-dissolving activity and IAA-producing capacity, can inhibit fungal pathogenic bacteria by secreting chitinase to degrade chitin, can increase the contents of available phosphorus and available potassium in tea garden soil, can improve the total potassium content in tea, can increase the density of tea shoots and the yield of tea, can increase the content of free amino acids in tea, and can reduce the content of tea polyphenols and the phenol-ammonia ratio in tea, thereby improving the quality of tea.

The invention relates to the technical field of food preservation and fresh keeping, in particular to a natural food preservative and a preparation method thereof.The natural food preservative is prepared by mixing lactic acid bacteria rhzomorph, nisin, lysozyme, epsilon-polylysine, plant extracts, microsphere compounds, antioxidants and deionized water, and the various effective ingredients play a synergistic effect, so that the natural food preservative is prepared. The food preservative is good in preservative and fresh-keeping effect, high in antibacterial activity and high in utilization rate of natural components. Gram-positive bacteria and Gram-negative bacteria are important reasons causing putrefaction of fruits, vegetables and meat products, antibacterial peptides-nisin and lactic acid bacteria rhzomorph in the food preservative can act on the Gram-positive bacteria, chitinase in the microsphere compound has a relatively strong inhibition effect on the Gram-negative bacteria, and the antibacterial peptides-nisin and lactic acid bacteria rhzomorph can act on the Gram-positive bacteria. After being compounded with the plant extract, the compound has a relatively good inhibition effect on food spoilage and has a wide application prospect.

The invention relates to the technical field of pesticides, in particular to a pesticide composition for inhibiting pre-harvest sprouting, a preparation and application of the pesticide composition. The composition comprises the following components in parts by weight: 2-8 parts of p-chlorophenoxyacetic acid or an agronomically acceptable salt thereof, 10-50 parts of an enzyme preparation and 10-50 parts of a copper preparation, and the enzyme preparation comprises lysozyme and chitinase in a weight ratio of 1: 1. Through scientific compatibility of all the components, pre-harvest sprouting can be inhibited, and medication is safe.

The invention relates to the technical field of feed additives, in particular to a compound enzyme preparation for indoor aquaculture of marine economic animals. The compound enzyme preparation is prepared from the following components in parts by weight: 13 to 22 parts of protease, 8 to 15 parts of amylase, 12 to 26 parts of xylanase, 10 to 20 parts of phytase, 8 to 13 parts of beta-glucanase, 5 to 10 parts of cellulase, 5 to 10 parts of pectinase, 5 to 10 parts of chitinase and 3 to 5 parts of glucose oxidase. The preparation process comprises the following steps: step 1, single enzyme pretreatment; step 2, grouping and compounding; step 3, mixing and sieving; and 4, packaging a finished product. The phytase, the beta-glucanase, the chitinase and the glucose oxidase are added, so that the feed additive can adapt to different types of feeds, and meanwhile, through the synergistic effect of the phytase, the beta-glucanase, the chitinase and the glucose oxidase, the utilization rate of the feeds can be increased, the culture environment is optimized, and green ecological aquaculture is realized.