197 results about "Chitinase" patented technology

The invention discloses a compound enzyme preparation for improving the absorption and utilization rate of a fertilizer and a preparation method thereof, and belongs to the technical field of enzyme preparations. The compound enzyme preparation is prepared from the following components in parts by weight: 100 parts of purified water, 6 to 8 parts of modified cellulase, 10 to 12 parts of immobilized protease, 10 to 12 parts of phosphatase, 10 to 14 parts of urease, 8 to 10 parts of nitrate reductase, 10 to 12 parts of pectinase, 8 to 10 parts of chitinase, 6 to 8 parts of xylanase, 10 to 12 parts of beta-glucosidase, 10 to 12 parts of superoxide dismutase and 4.5 to 6 parts of a microbial agent, the modified cellulase is obtained by chemically modifying cellulase; the immobilized protease is prepared by the following steps: adsorbing activated and coupled modified polylactic acid and a protease solution, crosslinking with glutaraldehyde, filtering, washing and drying. The compound enzyme preparation improves the fertilizer absorption and utilization rate of plants.

The invention relates to the technical field of microorganisms and bio-organic fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating rhizome diseases of horseradish and application of the bio-organic fertilizer, the bio-organic fertilizer comprises the following components: component A: a bacillus velezensis microbial inoculum with the preservation number of CCTCC NO: M 2022923 and a trichoderma yunnanense microbial inoculum with the preservation number of CCTCC NO: M 2025645; the component B is prepared from soybean meal, vinegar residues, charcoal, wollastonite powder and allicin. The biological organic fertilizer disclosed by the invention can be used for increasing the content of growth hormones such as salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) and phytoalexin as well as the activity of chitinase and beta-1, 3-glucanase of a wasabi root system, and has a relatively good prevention and treatment effect on a black heart disease caused by infection of phoma wabi Yokog and a soft rot disease caused by infection of pectobacterium carotovorum subsp. Carotovorum (Pcc). The biological organic fertilizer disclosed by the invention has a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi Yokog and a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi subsp. Carotovorum (Pcc).

The invention discloses an antibacterial peptide fragment intercepted from chitinase IDGF4 protein and application of the antibacterial peptide fragment in preparation of antibacterial drugs. According to the invention, short peptide fragments in chitinase IDGF4 protein are screened, three candidate peptides are obtained through chemical synthesis, the antibacterial performance of the candidate peptides is systematically evaluated, and the evaluation result shows that the peptide fragment with the amino acid sequence as shown in SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg / mL; the antibacterial effect of the compound is confirmed in inhibition zone experiments, enzyme-labeled turbidimetry and growth curve monitoring, and the compound shows stable and remarkable antibacterial activity and can be used as a novel antibacterial molecule; the invention provides a new candidate sequence for developing the antibacterial peptide with high efficiency and low drug resistance risk, and has application potential in the aspect of preparing clinical antibacterial drugs.

The invention relates to a composite microbial agent suitable for saline-alkali soil remediation of sorghum production in northeast alpine regions, and a preparation method and application thereof. The composite microbial agent comprises bacillus subtilis, bacillus mucilaginosus and pseudomonas fluorescens. Bacillus mucilaginosus capable of efficiently producing polyglutamic acid and potassium and pseudomonas fluorescens capable of secreting chitinase are separated and screened from obstacle soil in northeast alpine regions, and field trials are carried out in the obstacle soil (coastal saline-alkali soil) in the northeast alpine regions. The low-temperature slow-release fertilizer has the unique characteristics of secreting polyglutamic acid, potassium and chitinase under the low-temperature condition, is low in cost, and can effectively improve the physical and chemical properties of soil, increase the seed emergence rate, stimulate the root growth and enhance the salt resistance and alkali resistance of seedlings after being applied.

The invention provides a high-activity chitinase mutant and application thereof in degradation of crustacean waste, and belongs to the technical field of enzyme engineering and agricultural waste conversion. According to the invention, chitinase PbChi from Paenibacillus barengltzii is subjected to molecular modification, and a plurality of chitinase mutants with high activity are successfully constructed by increasing the flexibility of a connexin, changing steric hindrance at a substrate catalytic crack inlet and changing catalytic residues in a beta sheet layer in a catalytic structural domain through calculation design and experimental verification; the chitinase mutant can effectively degrade shrimp shell meal, so that carapace waste is effectively utilized, and the chitinase mutant has a wide application prospect in the biological feed industry.

The invention relates to the technical field of fertilizers, and particularly discloses a bacterial strain capable of reducing the function of vomitoxin of wheat and corn and application of the bacterial strain, the bacterial strain capable of reducing the function of vomitoxin of wheat and corn is applied to preparation of a fertilizer, 10%-90% of bacillus velezensis XNYF-1 fermentation liquor (A liquor) and 10%-90% of pseudomonas fluorescens XNYF-2 fermentation liquor (B liquor) are mixed to prepare AB mother liquor, and the AB mother liquor is used for preparing the fertilizer. The XNYF-1 and the XNYF-2 are two functional microbial strains which are subjected to screening mutagenesis and can be used for producing chitinase, iturin, various phenazine derivatives and biological surfactin respectively, and the XNYF-1 and the XNYF-2 can be used for producing the chitinase, the iturin, the various phenazine derivatives and the biological surfactin respectively. And the metabolite has a good biocontrol effect.

The invention relates to a skin wound repairing material and a preparation method thereof, and belongs to the technical field of biological medicines. The repair material disclosed by the invention contains palmitoyl tripeptide-1, methacrylated collagen, cuttlebone extract, recombinant human epidermal growth factor lipidosome, polydopamine nanoparticles, hyaluronic acid, sodium alginate modified silk fibroin and the like in a specific proportion. Wherein the cuttlebone extract is a product which is obtained by carrying out common enzymolysis on cuttlebone through chitinase and nattokinase and then carrying out step-by-step enzymolysis on the cuttlebone through gelatinase and lumbrukinase and is less than 5kDa. The components are prepared into an efficient product by adopting a special method, the stability of the components is improved, the problem that the activity is difficult to maintain is solved, and the skin wound repairing effect is good through reasonable compatibility.

The invention discloses application of oilseed rape chitinase EP3 and a coding gene thereof in improving clubroot resistance of cruciferous plants, and belongs to the technical field of molecular biology. The invention discloses the application of the rape chitinase EP3 in improving the clubroot resistance of plants for the first time. The amino acid sequence of the rape chitinase EP3 is as shown in SEQ ID NO.2; the nucleotide sequence of the coding gene EP3 is shown as SEQ ID NO. 1. Experimental results show that by preparing agrobacterium containing an EP3 gene overexpression vector and using a treating fluid containing the agrobacterium to perform seed soaking and root irrigation treatment, the EP3 gene is overexpressed in a rape plant, and the clubroot resistance of the rape can be remarkably improved. The invention provides a new gene resource and a safe, efficient, simple, convenient and universal prevention and treatment method for preventing and treating clubroot of cruciferous plants, and has remarkable agricultural application value and wide application prospect.

The invention discloses bacillus Witzmannii for inhibiting phytopathogen and application of the bacillus Witzmannii, relates to the field of biological prevention and control, and aims to solve the problem that chemical prevention and control methods of phytopathogen cause negative effects on ecological environment and human health. The bacillus wiedmannii is bacillus wiedmannii SJZ and is preserved in the China Center for Type Culture Collection, the preservation date is December 23, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20242884. The bacillus vetzmannii disclosed by the invention can be used for inhibiting sclerotinia sclerotiorum and solanum nigrum. And protease, cellulase and chitinase can be secreted. The bacillus vetzmannii is used for nitrogen fixation and can secrete siderophores and IAA (indoleacetic acid).

The invention provides mangrove chitin bacillus FCG-7 and application of the mangrove chitin bacillus FCG-7 in preparation of N-acetylglucosamine, and belongs to the technical field of microorganisms, the mangrove chitin bacillus FCG-7 provided by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 63488. The strain provided by the invention has good low-temperature resistance and extracellular chitinase production capability, and can efficiently degrade chitin under a low-temperature condition to produce N-acetylglucosamine with an excellent biological effect; and the strain is still stable after passage, and is expected to be applied to the production of high-added-value N-acetylglucosamine by using chitin biomass as a raw material under a low-temperature condition.

The invention provides a cordyceps militaris mycelium and a preparation method and application thereof, and relates to the technical field of biological materials. According to the invention, chitinase is added into the cordyceps militaris mycelium material to carry out enzymolysis on chitin to obtain chitooligosaccharide and N-acetylglucosamine, so that beta-glucan in a mycelium framework is dissolved out, and the grafting efficiency of the cordyceps militaris mycelium material is improved; furthermore, the antibacterial effect of the antibacterial material is improved by carrying out grafting reaction on the cordyceps militaris mycelium material and an exogenous monomer antibacterial peptide which has antibacterial performance and contains carboxyl groups.

The invention belongs to the technical field of microorganisms, and particularly discloses a strain of Paenibacillus amyloliquefaciens (Paenibacillus amyloliquefaciens) capable of increasing the yield of tea leaves and improving the quality of the tea leaves and an application of the Paenibacillus amyloliquefaciens (Paenibacillus amyloliquefaciens) capable of increasing the yield of the tea leaves and improving the quality of the tea leaves. The preservation number of the chitin-dissolving paenibacillus is CCTCC NO: M 20241893, and the strain has efficient potassium-dissolving activity, strong organophosphorus-dissolving activity and IAA (Indoleacetic Acid) production capacity, and can degrade chitin by secreting chitinase, so that the strain has certain inhibition capacity on fungal pathogenic bacteria, and can be applied to the field of agricultural production. The bacterial strain can increase the content of rapidly available phosphorus and rapidly available potassium in tea garden soil, increase the total potassium content of tea leaves, increase the density of tea buds and the yield of the tea leaves, increase the content of free amino acids in the tea leaves and reduce the content of tea polyphenols and the phenol-ammonia ratio of the tea leaves, so that the quality of the tea leaves is improved.

The present invention discloses a chitinase-producing desert bacillus and its application, and relates to the field of microbial technology. The present invention discovers a new microbial strain, which is identified as belonging to the genus Desert Bacillus and is named Desert Bacillus sp.MYHB_202305ChI. Experimental verification shows that the strain has the ability to tolerate high salt and secrete chitinase.

The invention discloses a lilium lancifolium LlELP1 gene, the nucleotide sequence of the lilium lancifolium LlELP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of expression protein of the lilium lancifolium LlELP1 gene is as shown in SEQ ID NO: 2; according to a specific primer pair of the lilium lancifolium LlELP1 gene, the nucleotide sequences of the LlELP1-F and the LlELP1-R are as shown in SEQ ID NO: 3 and SEQ ID NO: 4. The invention also discloses an application of the lilium lancifolium LlELP1 gene in lilium lancifolium breeding. The lilium lancifolium LlELP1 gene can promote the generation process of bulbils of lilium lancifolium and increase the number of bulbils. The chitinase related gene capable of regulating and controlling the bulbil generation number is excavated for the first time, directional selection of the bulbil generation number is facilitated, and reference is provided for molecular breeding.

The invention belongs to the technical field of chitosan oligosaccharide preparation, and particularly relates to a method for preparing chitosan oligosaccharide based on compound enzyme. According to the method for preparing the chitosan oligosaccharide based on the compound enzyme, a multi-enzyme synergistic system is matched with the design of a specific protective agent, chitosanase and chitinase can cooperatively cut beta-1, 4 glycosidic bonds of chitosan and control molecular weight distribution of products, meanwhile, chitinase can directionally cut the beta-1, 4 bonds at the tail end of chitosan, and the molecular weight distribution of the products is controlled. The papain is used for removing protein impurities in the chitosan to realize accurate control of the polymerization degree. Besides, a specific protective agent matched with the multi-enzyme synergistic system is specifically designed, the specific protective agent and the multi-enzyme composition act together, the molecular weight distribution width can be remarkably reduced, the yield and the DP 4-8 proportion can be increased, meanwhile, physical field ultrasonic assistance is added in the enzymolysis treatment process, and the enzymolysis treatment efficiency is improved. The combination of the substrate and the enzyme is further promoted, and the reaction time is shortened.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to a high-efficiency yield-increasing liquid fertilizer containing enzymolysis nereis and a preparation method thereof.The method comprises the steps that the nereis is cleaned and smashed, subjected to freeze-thaw cycle pretreatment and then subjected to enzymolysis through a compound protease preparation containing protease and chitinase under the alkalescence condition, and the liquid fertilizer containing the enzymolysis nereis and the chitinase is obtained; carrying out solid-liquid separation on the obtained enzymatic hydrolysate to obtain enzymatic clam worm liquid; the preparation method comprises the following steps: mixing urea, a phosphorus-potassium fertilizer, trace elements, potassium fulvate and polyaspartic acid in a preparation tank, and then adding an N-carbamoyl-L-glutamic acid-chitosan grafted copolymer and selenocysteine-alginic acid nanoparticles to obtain a nutrient solution; and finally, mixing the enzymolysis nereis solution with the nutrient solution and the plant growth regulator to obtain a finished product. Active substances in the nereis are fully released through a biological enzymolysis technology, the prepared liquid fertilizer has the functions of nutrition supply, physiological regulation and soil improvement, crop growth can be remarkably promoted, the yield can be remarkably increased, and high-value utilization of marine biological resources is achieved.

Disclosed in the present invention are a Serratia marcescens strain, and the use thereof in co-production of prodigiosin, chitinase and lipase. The Serratia marcescens strain is classified and named Serratia marcescens LT-7 and has been preserved in China Center for Type Culture Collection with the accession number CCTCC NO: M20231646, the preservation date being September 07 2023. The strain synthesizes prodigiosin, chitinase and lipase by means of fermentation by using molasses as a carbon source and tryptone as a nitrogen source; and during a coproduction process, the concentration of prodigiosin accumulated in a culture medium reaches up to 20.70 g / L, the activity of chitinase is 49.78 U / mL and the activity of the lipase is 86.92 U / mL. The operation method disclosed by the present invention is simple, effective and low-cost, has a great industrial production prospect, and provides an innovative process for the biosynthesis of prodigiosin, chitinase and lipase.

The invention discloses a Chinese herbal medicine leavening, a preparation method thereof and application of the Chinese herbal medicine leavening in cosmetics, and particularly relates to the technical field of cosmetics. Comprising the following raw materials in parts by weight: 12-18 parts of radix astragali, 8-12 parts of radix ampelopsis, 10-15 parts of radix saposhnikoviae, 18-22 parts of rhizoma chuanxiong, 6-9 parts of bitter almond seeds, 18-22 parts of radix salviae miltiorrhizae, 7-10 parts of herba centellae, 6-9 parts of folium artemisiae argyi, 7-10 parts of fructus gardeniae, 4-6 parts of flos sophorae immaturus, 5-8 parts of fructus citri, 12-15 parts of poria cocos and 1.5-2.5 parts of a dendrobium officinale extract. The composition can synergistically exert moisturizing, immune regulation and anti-allergy effects with other raw material components to form an anti-allergy, anti-oxidation and repair efficacy closed loop, a process chain from aspergillus oryzae high-enzyme-activity solid fermentation to double-bacterium liquid symbiosis to chitinase directional enzymolysis can synergistically improve the yield of active matters, and the situation that traditional anti-allergy components have no hair growth effect is broken through, so that the anti-allergy and anti-allergy effects are achieved. Due to the limitation that the hair restorer stimulates sensitive skin, the hair growing and double effects are integrated, and the hair restorer is suitable for preparing cosmetics for resisting allergy, resisting oxidation and repairing barriers and is especially suitable for sensitive skin care and scalp care.

The invention relates to the technical field of agricultural microorganisms, in particular to paenibacillus brevis KF-B6 and application thereof, in particular to application in prevention and treatment of continuous cropping obstacles of apples. The preservation number of the paenibacillus brevis KF-B6 is CGMCC (China General Microbiological Culture Collection Center) No.32131. The paenibacillus brevis KF-B6 has the compound functions of quickly producing EPS (Expandable Polystyrene) at high yield, producing chitinase at high yield, producing water-soluble and volatile antibacterial substances, decomposing macromolecular organic matters and the like. The application of the bacterium, the microbial agent and the compound microbial fertilizer thereof can effectively improve the number of bacteria in rhizosphere soil, reduce the number of pathogenic fungi, increase the proportion of the bacteria to the fungi and promote the restoration of a soil microbial ecosystem of a continuous cropping obstacle apple orchard; the proportion of water-stable aggregates is increased, the soil organic matter content is increased, the soil enzyme activity is improved, and the soil is improved; the survival rate of planted saplings in the new variety updating process of the continuous cropping obstacle old orchard is increased, the plant root activity and plant growth are promoted, and the apple continuous cropping obstacle is comprehensively relieved and prevented.

The invention discloses a method for synergistically degrading chitin by multiple enzymes, which comprises the following steps: adding lysing polysaccharide monooxygenase OsLPMO10A, chitinase and beta-N-acetylglucosaminidase Sohex into an alpha-chitin solution, and carrying out enzymolysis to prepare GlcNAc; the amino acid sequence of the beta-N-acetylglucosaminidase Sohex is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention further discloses the beta-N-acetylglucosaminidase Sohex and application of the beta-N-acetylglucosaminidase Sohex in degradation of chitin, preparation of GlcNAc and synthesis of lactose-N-trisaccharide II. The invention also discloses a method for synthesizing the lactose-N-trisaccharide II. The beta-N-acetylglucosaminidase Sohex with high hydrolytic activity and promotion effect is excavated, the beta-N-acetylglucosaminidase Sohex is combined with lysing polysaccharide monooxygenase and chitinase to be applied to degradation of alpha-chitin to prepare GlcNAc, and the beta-N-acetylglucosaminidase Sohex has very important environmental protection and industrial significance and has important industrial application value and economic value.

The invention discloses a novel brevibacterium root rot resistant species of Huanglonglong and application of the novel brevibacterium root rot resistant species. The invention provides a Huanglonglong brachybacterium root rot resistant strain, which is a Huanglonglong brachybacterium root rot resistant strain, the strain number of the Huanglonglong brachybacterium root rot resistant strain is BS409, and the registration number of the Huanglonglong brachybacterium root rot resistant strain in the China General Microbiological Culture Collection Center is CGMCC No.34201. The invention further provides a preparation method of the Huanglonglong brachybacterium root rot resistant strain. The strain BS409 provided by the invention is a new species of brevibacterium, can generate chitinase and hydrolyze chitin, and has inhibitory activity on root rot pathogenic bacteria fusarium equiseti (Fusarium equiseti). The results indicate that the strain BS409 has good potential in the aspect of biological prevention and control of root rot of medicinal plants (such as bighead atractylodes rhizome, pseudo-ginseng and ginseng), and can also be used in the aspects of soil remediation, soil improvement and the like of planting fields of bighead atractylodes rhizome, pseudo-ginseng and ginseng.

The invention relates to a composition for bone repair and growth and a preparation method thereof, and belongs to the technical field of medicines, the composition comprises a glue layer extracted peptide in sandjellyfish and a tabasheer-grifola frondosa composite bacterial peptide; the extract peptide of the glue layer in the sand jellyfish contains a product between 500Da and 2000Da, wherein the product is obtained by sequentially carrying out reaction on the glue layer in the sand jellyfish through lumbrukinase, serrapeptase and glutamine transaminase; the tabasheer-grifola frondosa composite bacterial peptide contains a product between 500Da and 2000Da, which is obtained by performing common enzymolysis on tabasheer mushrooms and grifola frondosa mushrooms through cellulase and chitinase, then performing common enzymolysis on the tabasheer mushrooms and the grifola frondosa mushrooms through nattokinase and serrapeptase and finally performing common reaction on aminopeptidase and glutamine transaminase. According to the invention, a special method is adopted to prepare the glue layer extracted peptide and the tabasheer-grifola frondosa compound bacterium peptide in the sandfish, and the glue layer extracted peptide and the tabasheer-grifola frondosa compound bacterium peptide are matched for use to achieve good biological activity, stability and absorption and utilization rate, so that the defects of the existing skeleton repair and growth medicines are overcome, and the repair and growth of skeletons are effectively promoted.

The invention provides a disease-resistant and growth-promoting aspergillus ochraceus AF-P9 and application thereof, and belongs to the technical field of microorganisms. The aspergillus ochraceus AF-P9 provided by the invention has salt resistance and alkali resistance, has the capabilities of producing siderophores and secreting chitinase, and has an efficient inhibition effect on a plurality of pathogenic bacteria such as fusarium oxysporum, fusarium oxysporum, fusarium tripteroides, pythium miscanthus, avena minuta, phomopsis divaricata, sclerotinia sclerotiorum, phytophthora and the like; the composition can be applied to prevention and treatment of rice damping-off. Furthermore, the metabolite of the aspergillus ochraceus AF-P9 is analyzed to obtain that the aspergillus ochraceus AF-P9 can generate a tricyclic cyclohexanone compound, a cyclohexene compound with a hydroxyl group, a phenanthrone-like compound with a hydroxyl group, a benzene ring compound with two long-chain esters, a cyclohexene compound with four parallel ring structures and the like. The aspergillus ochraceus AF-P9 provided by the invention provides a strain resource for biological prevention and control of diseases.

The present application relates to "application of a compound in soybean cyst nematode chitinase inhibitor", belong to the technical field of leguminous plant symbiotic protection, the application of the compound and its pharmaceutically acceptable salt in soybean cyst nematode chitinase inhibitor, the present application is evaluated and researched through the inhibitory activity of compound by screening work, the inhibitor is screened finally, the inhibitor is likely to have good application prospect in controlling soybean cyst nematode disease aspect.The present application also provides a kind of chitinase inhibitor with the function of inhibiting the establishment of symbiosis of soybean cyst nematode to destroy, the number of nodule obtained by processing is flat with the number when only inoculating rhizobium, the inhibitory effect of Hg-CHI-1 on rhizobium nodule is completely restored, and it has good application prospect in the aspect of leguminous plant symbiotic protection.

This invention discloses a chitinase-producing Bacillus albus A01 and its application in the fermentation of shrimp and crab solid waste. The chitinase-producing Bacillus albus is named... Bacillus albus A01, with accession number CCTCC: M 20252571; the 16S rDNA gene sequence of this chitinase-producing Bacillus albus strain A01 is shown in SEQ ID NO.1. The fermentation agent of this strain can effectively degrade the chitin content in shrimp and crab solid waste, and improve the nutritional value of shrimp and crab fermented powder through solid-state fermentation. This invention solves the problem of unsatisfactory fermentation effects of existing feed fermentation strains, and has great application potential for improving the feed nutritional quality of shrimp and crab fermented powder and developing feed resources.

The present application relates to a kind of organic water-soluble fertilizer and its preparation method, which uses fish waste and shell waste as raw material, after being crushed and sterilized, enzymatic hydrolysis is carried out using acid protease and chitinase to obtain enzymatic hydrolysate;Add molasses to the enzymatic hydrolysate, and inoculate mixed bacterial agent for primary fermentation to obtain fermentation liquor A;Add yeast, neutral protease and sodium alginate to the fermentation liquor A, and carry out secondary fermentation under stirring to obtain fermentation liquor B;After treating the fermentation liquor B, add chitosan to obtain the organic water-soluble fertilizer;The organic fertilizer prepared by the method of the present application can utilize shell waste, and the obtained organic water-soluble fertilizer can improve its water-retaining and fertilizer-retaining performance in sandy soil while meeting the standard requirements of amino acid water-soluble fertilizer.

The invention discloses chitinase derived from myxococcus flavus and application of the chitinase. The amino acid sequence of the chitinase ChitM disclosed by the invention is shown as the 22 -406 amino acid sequence of SEQ ID NO. 2. The chitinase ChitM disclosed by the invention has a remarkable splitting action on plant pathogenic fungi such as banana disc elongispora, banana fusarium oxysporum, tomato fusarium oxysporum, magnaporthe oryzae and botrytis cinerea, and when the encoding gene of the chitinase ChitM is transferred into arabidopsis thaliana, the resistance of the arabidopsis thaliana with the transferred ChitM gene to botrytis cinerea is obviously improved compared with that of wild arabidopsis thaliana; besides, the activity of ChitM is compared with that of commercial chitinase C.Chit, it is found that when the substrates are chitin and shrimp shells, the enzyme activity of the two is not obviously different, when the substrates are chitosan, the activity of ChitM is obviously higher than that of C.Chit, and when the substrates are soluble chitosan and colloidal chitin, the enzyme activity of C.Chit is obviously higher than that of ChitM, and when the substrates are soluble chitosan and colloidal chitin, the enzyme activity of ChitM is obviously higher than that of C.Chit. The results show that the enzyme has great application potential in the aspects of biological prevention and control of plant fungal diseases, disease resistance, breeding and environmental protection.

The application belongs to the technical field of agricultural fertilizer, and particularly relates to a high-efficiency yield-increasing liquid fertilizer containing enzymatic nereid and a preparation method thereof, which comprises the following steps: washing and crushing nereid, and then performing freeze-thaw cycle pretreatment; under weak alkaline conditions, a complex protease preparation containing protease and chitinase is used for enzymolysis, and an enzymatic nereid liquid is obtained through solid-liquid separation; urea, phosphate-potassium fertilizer, trace elements, potassium fulvate and polyaspartic acid are mixed in a preparation tank, and then N-carbamoyl-L-glutamic acid-chitosan graft copolymer and seleno-cysteine-alginic acid nanometer microspheres are added to obtain a nutrient liquid; finally, the enzymatic nereid liquid, the nutrient liquid and a plant growth regulator are mixed to obtain a finished product. Through the biological enzymolysis technology, the active substances in the nereid are fully released, the prepared liquid fertilizer has the functions of nutrient supply, physiological regulation and soil improvement, can significantly promote crop growth and increase yield, and realizes high-value utilization of marine biological resources.