455 results about "Chitinase" patented technology

The subject invention concerns materials and methods for controlling agricultural traits in plants that are mediated by the plant hormone ethylene. One aspect of the invention concerns a polynucleotide that comprises a sequence encoding a mutant ethylene receptor that is operably linked to a regulatory sequence that drives expression of the mutant receptor in a tissue-specific manner. In an exemplified embodiment, the mutant receptor sequence is an etr1-1 sequence, or a functional fragment or variant thereof, and the regulatory sequence is a promoter sequence from a cotton chitinase gene that can promote expression of the mutant ethylene receptor in abscission zone tissue of a plant. The subject invention also concerns plants and plant tissue transformed with the polynucleotide of the subject invention. Plants expressing the polynucleotide of the subject invention do not drop their flowers in response to exposure to ethylene.

The present invention provides a nucleic acid encoding a chitinase and methods for it to enhance resistance of plants to fungal infections.

The invention discloses an insect bioreactor capable of expressing multiple exogenous genes, and a construction method and application thereof. The construction method comprises the following steps: (1) introducing multicopy high-efficiency bacteria DNA (deoxyribonucleic acid) replication initiator into chitinase and cysteine proteinase genes of a baculovirus genome to obtain a baculovirus shuttle plasmid; (2) replacing virus duplicated essential gene downstream the polyhedrosis gene of the baculovirus shuttle plasmid with antibiotic gene to obtain a baculovirus plasmid DNA; and (3) replacingother virus duplicated and infected nonessential genes in the baculovirus shuttle plasmid with reverse selection marker gene to obtain the insect bioreactor. The antibiotic gene or reverse selection marker gene in the insect bioreactor which is constructed by replacing the exogenous target genes can express multiple exogenous genes in a host insect or insect cell. The insect bioreactor disclosed by the invention can efficiently expressing one or more exogenous genes in an insect body at the same time, and can produce massive recombinant proteins at low cost.

The invention discloses a strain of Streptomyces and application thereof. The biocontrol Streptomyces is blastmycin Streptomyces and its strain number is JZB130180, which is registered in the general microbiological center of China Committee for Culture Collection of Microorganisms with CGMCC No. 13270. The strain had significant antagonistic effect on 14 kinds of plant pathogenic fungi and 6 kinds of plant pathogenic bacteria in agricultural production, and has wide antimicrobial spectrum. The strain produces chitinase, cellulase, protease and selenium, and has the basic characteristics of biocontrol bacteria. The fermentation broth of this strain has strong control effect on tomato gray mold and peach brown rot, and has significant promotion action on the tomato plants, which indicated that the strain had a broad application prospect in the field of biological control of plant diseases.

A planting system involving a non-damaging stimulus (3) which is placed in the vicinity of a propagule (2) causes a naturally defensive substance (5) to be produced by the propagule. This substance may exist within an encapsulant (1) for a period of time so that the propagule may have enhanced disease control until it develops sufficiently to fend for itself. In one embodiment a large encapsulant surrounds a potato tuber. This encapsulant may include chitin to cause the release of chitinase through intermediate stimulants such as mRNA and activated carbon to absorb or release particular substances. The encapsulant is bound through the use of pentosan which acts with flaked chitin to achieve an outer casing and further protect the propagule both mechanically and chemically.

The present invention provides compositions and methods for the detection, treatment, and prevention of a kidney injury. The invention relates to the discovery that chitinase 3-like 1 / Brp-39 / YKL-40 serves as both a biomarker for the degree of kidney injury and a critical mediator of a reparative response in the kidney.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The present invention relates to chitin oligose preparing process, and is especially enzyme generating fermentation process to degrade chitin colloid for preparing bioactive chitin oligose. The preparation process has simple technological path, effective inhibition of further degradation of bioactive chitin oligose, reuse of degrading enzyme and other advantages, and is suitable for industrial production. The preparation process includes: culturing Aeromonascaviae strain to obtain fermented liquid, centrifugally separating the fermented liquid to obtain enzyme supernatant; preparing chitin colloid; compounding chitin colloid buffer solution in an enzyme reactor and adding abacterial enzyme liquid for enzymolysis to obtain enzymolyzed chitin oligose liquid; four stages of membrane separation to obtain concentrate and spray drying to obtain bioactive chitin oligose product.

The invention provides chitinase gene sequences and application of dsRNA thereof, which can silence specific chitinase genes and make insects die of retarded development. By cloning, sequencing and analyzing expression of the chitinase genes of a plurality of locusta migratoria manilensis, the chitinase genes with a sequence of SEQ IDNO:1 are obtained; and the chitinase genes with sequences of SEQ ID NO:2 and SEQ ID NO:3 are selected and used for synthesizing the dsRNA. After the dsRNA is injected into a coelom of an insect, the insect shows difficult molting in the development process and dies. The chitinase gene sequences provide a basis for the effective prevention and control of the insects based on RNA interference, and provide a new approach for the development of a safe and pollution-free method for preventing and controlling the insects.

The invention discloses an enzymatic extraction method for auricularia auricula polysaccharides, which comprises the following steps of: mechanically and coarsely grinding dried auricularia auricula, sieving, mixing according to a material-to-water ratio of 1:(50-200), and extracting at the temperature of between 35 and 50 DEG C and the pH of between 5.0 and 9.0 under the action of chitinase for 60 to 180 minutes, wherein the addition amount of the chitinase is 100 to 600U / g; raising the extraction temperature to be between 80 and 95 DEG C, extracting for 60 to 180 minutes to obtain extract, and centrifuging to obtain supernatant and precipitates; and adding ethanol into the supernatant to precipitate polysaccharides, performing centrifugal separation to obtain precipitates, dissolving the precipitates in water, concentrating to remove residual ethanol, adding a mixture of diatomite and activated carbon into concentrated solution, mixing, fully stirring, filtering, performing membrane filtration on filtrate by using a polysulfone membrane of 30 to 100kD, collecting macromolecular concentrated solution which does not filter through the membrane, and drying the macromolecular concentrated solution to obtain the auricularia auricula polysaccharides. The prepared products have high purity and bioactivity; and the polysaccharide content is over 40 percent.

The invention discloses Bacillus atrophaeus and an application thereof. The Bacillus atrophaeus JZB120050CGMCC No.10919 has stable, efficient and broad-spectrum antibacterial properties. The Bacillus atrophaeus can generate a biological membrane, has strong capability of generating siderophore, chitinase, cellulase and protease, which shows that the strain has relatively strong biocontrol potential. The control effect of fermentation liquor of the strain to tomatoes inoculated with botrytis cinerea for culturing 11d is 100% (the control effect of a 5-time diluent treatment group of JZB120050 strain fermentation liquor is 48%, the control effect of a treatment group of JZB120028 strain fermentation liquor is 50%, the control effect of a 5-time diluent treatment group of JZB120028 strain fermentation liquor is 26%, and the control effects of a blank control group, a 1000-time liquid treatment group of 3% polyoxin, and 1000-time liquid treatment group of 50% carbendazim are 0%), which shows that the strain has an effective inhibitory action on the botrytis cinerea. The strain is safe to human and domestic animals, does not have environmental pollution problems, is simple in culture condition, easy to store and suitable for industrial production, and has a good development application prospect.

Plants are provided with improved resistance against pathogenic fungi. They are genetically transformed with one or more polynucleotides which essentially comprise one or more genes encoding plant chitinases and beta -1,3-glucanases. Preferred are the intracellular forms of the said hydrolytic enzymes, especially preferred are those forms which are targeted to the apoplastic space of the plant by virtue of the modification of the genes encoding the said enzymes. Particularly preferred are plants exhibiting a relative overexpression of at least one gene encoding a chitinase and one gene encoding a beta -1,3-glucanase.

The invention provides a sequence of locust I type chitinase gene (cht5), and an application of dsRNA of the locust I chitinase gene. The dsRNA can silence the I type chitinase gene to make locusts dead. The I type chitinase gene with the sequence represented by SEQ ID NO:1 is obtained by cloning the locust I type chitinase gene, and sequencing; and a gene fragment with the sequence represented by SEQ ID NO:2 is selected to synthesize double strand RAN (dsRNA). After the dsRNA is injected to the body cavity of the locust, the locust has delayed growth, and cannot successfully molt to enter a next age period, so the locust is dead. A new target is provided for the RNA interference based insect control in the invention.

The invention provides a biological wall-breaking method of aseptic ganoderma lucidium spore powder. The method comprises: adding cordyceps militaris into the aseptic ganoderma lucidium spore powder for solid fermentation, and making the ganoderma lucidium spore powder to break the wall. By using the chitinase-secreting cordyceps militaris as a wall-degrading fungus, effective and safe wall breaking for ganoderma lucidium spore can be carried out, and thereby the active substances of the ganoderma lucidium powder are furthest reserved and protected, and are in favor of human body absorption and utilization; and not only the cordyceps militaris fermentation process makes ganoderma lucidium spore to break the wall, but also the cordyceps militaris mycelium does not need removing, because cordyceps militaris is a beneficial microbe, and can generate active substances such as cordyceps polysaccharide, cordycepic acid, cordycepin and the like which can cooperatively reinforce the efficacies of the aseptic ganoderma lucidium spore powder.

The invention relates to a Trichoderma strain for antagonizing soil-borne disease, belonging to the field of biological control. The Trichoderma strain is Trichoderma asperellum ZJSX5003 CGMCC No.6480. The Trichoderma strain provided by the invention has the characteristics of high growth speed and high spore production amount; the in-vitro standoff pathogen inhibition rates are respectively up to 79.79%, 73.68% and 72.25%, the pathogen spore survival rates are respectively 0.00%, the in-vivo preventive effects for cucumber fusarium wilt and maize stem rot are respectively 85.08% and 66.67%, the enzyme activity of chitinase is 4.0783U, the enzyme activity of beta-1,3-glucanase is 0.8050U, the enzyme activity of extracellular proteinase is 4.8503U, and the content of silicone, terpene and carboxylic acid having antagonistic actions in antibiotic secondary metabolites is 44.44%. The Trichoderma strain provided by the invention has the advantages of high antagonism and high specificity. The biological pesticide produced from the strain can effectively control the soil-borne disease, thereby ensuring the stable increase of crop and vegetable yields.

The invention discloses a strain of brevibacillus laterosporus SNB10 for inhibiting riziocotinia solani. The brevibacillus laterosporus SNB10 was preserved in China General Microbiological Culture Collection Center on May 17, 2017, and the preservation number is 14171. The brevibacillus laterosporus SNB10 has the following characteristics of high inhibition effect on the riziocotinia solani and good prevention and treatment effect on rice sheath blight when being prepared into a biocontrol aqueous solution. The brevibacillus laterosporus SNB10 can produce chitinase, is wide in antibacterial spectrum, and has a certain inhibition effect on various plant pathogenic fungi and pathogenic bacteria. The brevibacillus laterosporus SNB10 does not have toxicity and pathogenicity, is safe for human and livestock, environmentally friendly, high in stress resistance and stable and durable in bacteriostatic activity, and has potential application prospect. The brevibacillus laterosporus SNB10 provides a microbial strain and a pesticide product thereof, wherein the microbial strain is non-toxic for human and livestock and friendly for the ecological environment, and has a good effect of preventing the rice sheath blight.

The invention discloses streptomyces alboniger capable of preventing diseases and promoting the growth and preparation and application of metabolites of the streptomyces alboniger. The strain number of the streptomyces alboniger is T22, the registration number in the China microbial strain preservation management committee general microbiological center is CGMCC No.14190. The streptomyces alboniger T22 has inhibition effects on botrytis cinerea, monilinia fructicola, colletotrichum capsici and the like of fruit and vegetable crops. Sterile fermentation filtrate of the streptomyces alboniger T22 can effectively inhibit the growth of strawberry botrytis cinerea hyphae, tomato botrytis cinerea hyphae and cucumber botrytis cinerea hyphae. The streptomyces alboniger can form a hydrolyzation circle on an enzyme production activity detection culture medium by producing cellulase and chitinase. It is clear that the streptomyces alboniger T22 can inhibit plant pathogenic fungi by producing antibiotic active substances and hydrolases. The sterile fermentation filtrate of the streptomyces alboniger T22 can significantly promote the elongation of tomato hypocotyls and / or radicles after being diluted appropriately, and the germination potential of tomato seeds is enhanced.

The invention discloses an efficient microbial agent for resisting insects, preventing diseases, increasing fertility and promoting growth. In order to solve the technical problem that the existing microbial agent does not have ideal effects of resisting insects, preventing diseases, increasing the fertility and promoting the growth. The technical scheme adopted by the invention is as follows: the microbial agent is obtained by implanting soybean protein, amino acid, fulvic acid and a medium trace element which are used as carriers into a mixture of ferment bacteria, endogenous bacillus and beauveria bassiana and fermenting for 48 to 60 hours at the temperature of between 38 and 46 DEG C; and diseases and insects are prevented and controlled through antibiotics and chitinase generated in the bacteria fermentation process according to the technical principle. The microbial agent has the technical effects that the soil can be improved, and the root system is stimulated to grow, so that crops well absorb required nutrients; the microbial agent has a good effect of resisting the infringement of root-knot nematode and stem eelworms, has quite positive promoting effects of swelling fruits and increasing the sweetness, has obvious promoting effects on crop growth, plant reinforcement, yellowing resistance, lodging resistance and rotting resistance, and integrates insect resistance, disease prevention, fertility increase and growth promotion; and the labor intensity is reduced, manpower and material resources are saved, and the production cost is reduced.

The invention relates to a Trichoderma strain for antagonizing soil-borne disease, belonging to the field of biological control. The Trichoderma strain is Trichoderma asperellum ZJSX5003 CGMCC No.6480. The Trichoderma strain provided by the invention has the characteristics of high growth speed and high spore production amount; the in-vitro standoff pathogen inhibition rates are respectively up to 79.79%, 73.68% and 72.25%, the pathogen spore survival rates are respectively 0.00%, the in-vivo preventive effects for cucumber fusarium wilt and maize stem rot are respectively 85.08% and 66.67%, the enzyme activity of chitinase is 4.0783U, the enzyme activity of beta-1,3-glucanase is 0.8050U, the enzyme activity of extracellular proteinase is 4.8503U, and the content of silicone, terpene and carboxylic acid having antagonistic actions in antibiotic secondary metabolites is 44.44%. The Trichoderma strain provided by the invention has the advantages of high antagonism and high specificity. The biological pesticide produced from the strain can effectively control the soil-borne disease, thereby ensuring the stable increase of crop and vegetable yields.

The invention aims to provide bacillus subtilis capable of producing chitinase and application thereof. The bacillus subtilis is obtained by plasma mutagenesis, has higher chitinase enzyme activity and protease enzyme activity, can be used as leavening agents, probiotics and preservatives, and can be widely applied to enzyme preparations and food industries. The preservation number of bacillus subtilis is CGMCC No.9166. The bacillus subtilis strain has relatively high chitinase enzyme activity and protease enzyme activity, is simple in nutrition requirement but has high fecundity, can be used as leavening agents, probiotics and preservatives, and has a wide application prospect.

The invention discloses a microbial preparation for preventing and controlling plant root knot nematodes and the application thereof. The preparation is mixed from Paecilomyces lilacinus, Bacillus subtilis, shell powder, shrimp shell powder, weathered coal humic acid and peat. The shell powder and shrimp shell powder are added to induce paecilomyces lilacinus to produce chitinase. The preparation can be stored at room temperature, and after storage for 90 days at 25 DEG C, the survival rate of paecilomyces lilacinus is more than 80%. The tomato pot experiment and cucumber field test prove that the preparation has remarkable effect on the control of root knot nematodes; at the same time, soil colonization test verify the Paecilomyces lilacinus in the preparation has good colonization condition in soil.

A composition for treating cancer is provided. The composition is a hexosaminidase covalently attached to a cancer cell targeting ligand and improves selectively of the hexosaminidase for tumor cells. In certain embodiments, the hexosaminidase is alternately chitinase (N-acetyl-glucosaminohydrolase), chitosanase, or N-acetyl-hexosaminidase and the targeting ligand is either a monoclonal antibody, an antibody fragment immunospecific to a tumor cell or cancer cell antigen, epidermal growth factor (EGF), fibroblast growth factor (FGF), transferrin, or folic acid. Also provided is a method for treating cancerous tumors comprising administering the composition to a patient that has a cancerous tumor.

The invention provides an organized enzyme biologic restorer (fertilizer), which can remove toxin in crops and toxin in fruits organizations. The polluted noxious crops can be restored to an original innoxious status, and the soil which comprises noxious substances can also be restored to the original innoxiuos status. Meanwhile, the invention also has the functions of increasing yield of crops and killing pests. The restorer mainly comprises urease and peroxydase generated by bacteria one, two, three, laccase generated by fungi one and two, and tyrosinase generated by ray fungi one and two. An active bacterium and organized enzyme cenobium agent is formed, which can effectively degrade noxious polluted substances; chitinase (chitinase) acts as insecticidal action, and rubisco enzyme can improve yield of the crops. The formulation of the restorer (fertilizer) consists of bio organic liquid or sorbent (accounts for 25 percent), active enzyme revulsant I and II preparation (accounts for 9 percent), acylase nitrogen source (accounts for 25 percent), organized enzyme auxiliary and amplifier (accounts for 16 percent) and inorganic fertilizer and accessories (accounts for 25 percent).

The invention relates a method of for preparing agrocybe cylindracea hydrolysate by using a compound enzyme method, comprising the following steps of: (1) washing agrocybe cylindracea, crushing and pulping; (2) adding water for mixing pulp according to the proportion that the agrocybe cylindracea to water is 1:2 to 1:5; (3) first enzymolysis: adding compound enzyme I which comprises cellulase, xylanase, beta-dextranase, mannase and chitinase, wherein the addition amount of the compound enzyme I is 0.05% to 0.3% of that of the agrocybe cylindracea, and carrying out enzymolysis for 2-4 hours at 45 DEC C to 55 DEG C; (4) second enzymolysis: adding compound enzyme II and carrying out enzymolysis for 2-4 hours at 50 DEC C to 60 DEG C; (5) boiling enzymatic hydrolysate and killing enzyme; and (6) adsorbing, filtering and concentrating to obtain transparent hydrolysate. In the method, a compound enzyme preparation is used for preparing the agrocybe cylindracea so as to fully give play to the synergistic interaction; and a subsection processing technology is adopted for leading bioactive substances in the agrocybe cylindracea to be released to the greatest extent.

The invention relates to an insecticidal and antifungal bacillus thuringiensis bacterial strain and the application. The bacterial strain is bacillus thuringiensis 519-1, which is called as Bt 519-1 for short, the collection number is CGMCC No. 2327, the bacterial strain carries the insecticidal protein coding genes of cry1Aa, cry1Ab, cry1Ac, cry1I, cry2 and the vip3A and 70 kDa chitinase coding gene chiB. The semi-lethal concentration (LC50) to spodoptera exigua of the spore crystal complex freeze-dried powder of the bacterial strain is only 8.8Mug / mL, and the bacterial strain can inhibit the growth of 8 common plant pathogen fungal myceliums and inhibit the germination of the fungal spores completely. The usage of the bacterial strain can produce the insecticidal and anti-disease multifunctional biological pesticides, which is used for the prevention and the treatment of lepidoptera major pest spodoptera exigua and bollworm; at the same time, the bacterial strain also has the inhibition function of the plant pathogen fungi.

The invention discloses a peripheral nerve regeneration tube and its manufacturing method, comprising: using the biodegradable material via the weave to attain the tube, and the surface of tube has chitinase and its derivant, a coating of nerve growth factor whose thickness is 0.01-0.5 mm, and the molecular weight of chitinase and its derivant is 50-0.5 million. The degradation cycle of said inventive peripheral nerve regeneration tube is synchronous to the peripheral nerve regeneration, and the invention can induce the nerve to grow quick with better elasticity and anchorage force, therefore, it can effectively improve the quality and speed of nerve regeneration. In addition, the invention can bridge connect the damaged nerve in 80 mm, to reach the advanced world level.

The invention provides a bacterial strain capable of producing chitinase and application of the bacterial strain to production of chitinase by fermenting crab shell. The bacterial strain is paenibacillus pasadenensis CS0611 and is preserved in China Center for Type Culture Collection; the preservation number is CCTCC M2014458; the preservation date is October 8, 2014. The bacterium is a start strain; the crab shell is used as a carbon source and a nitrogen source and is fermented for producing chitinase. The fermentation technology is simple; the production cost is low; the enzyme activity of the fermentative supernatant liquid can reach 211.3mU / mL; the wastes of shrimp and crab shells can be efficiently hydrolyzed; the pollution to the environment is reduced.

The invention belongs to the application field of microorganisms, in particular to a rapeseed pseudomonas with plant growth promoting effect. The specific technical scheme is as follows: a strain of rapeseed pseudomonas is deposited in the General Microbial Center of China Microbial Culture Collecting Management Committee on July 30, 2018, and the deposit number is CGMCC No. 16170. The rapeseed pseudomonas has excellent salt tolerance, can maintain strong vitality and excellent ability of secreting plant hormones at high salt concentration, and can secrete chitinase and degrade chitin, thus having certain effect on controlling plant fungal diseases. The results show that the strain of Pseudomonas campestris has good salt tolerance, and can be used for controlling plant fungal diseases. Atthe same time, the bacterium also has good potassium and phosphorus solubilization effect, and can be practically used in saline-alkali soil improvement.

The invention discloses a method for producing N-acetyl glucosamine by enzymatically degrading chitin. The method comprises the following steps: step 1, producing chitinase by microbial fermentation; step 2, obtaining a crude enzyme solution; step 3, concentrating the enzyme solution; step 4, producing N-acetyl glucosamine. The invention firstly provides the method for producing N-acetyl glucosamine by enzymatically degrading chitin, and an enzyme production medium is low in cost and short in production cycle. The material used in the step of concentrating the enzyme solution can be recycled, the operation process is easy, and the enzyme recovery is high. A measure of preventing bacterial contamination is adopted in the degradation process, so that the purpose that the N-acetyl glucosamine with high medical value is efficiently and quickly produced by using cheap chitin with low value as a raw material is achieved, and the method has significant economic and social benefits.