Biological wall-breaking method of powder

A technology of Ganoderma lucidum spore powder and seeds is applied in the field of biological wall breaking of Ganoderma lucidum spore powder, which can solve the problems of loss of active substances, difficult removal of exogenous enzymes and high cost.

Active Publication Date: 2013-10-09
ZHEJIANG ESSENCE FUNGI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the conventional physical and chemical method of breaking the wall of ganoderma lucidum spores, it is easy to cause the loss of active substances, while the enzymatic method of breaking the wall has problems such as high cost and difficulty in removing exogenous enzymes. The present invention provides a beneficial microorganism that can secrete chitinase as a Lysing bacteria of Ganoderma lucidum spores, a method for effectively and safely breaking the wall of Ganoderma lucidum spore powder

Method used

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Examples

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Embodiment 1

[0018] A method for biological wall breaking of Ganoderma lucidum spore powder, said method comprising the following steps:

[0019] (1) Using C 0 60 irradiation to sterilize Chizhi spore powder, the irradiation dose is 25kGy;

[0020] (2) Preparation of Cordyceps militaris seed culture medium: Inoculate the strain of Cordyceps militaris ACCC52244 into the liquid seed culture medium, culture at 18° C. and 150 r / min for 4 days, and set aside. Preparation of liquid seed culture solution: 200g potatoes, cut into small pieces, add 1000ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000ml, add 20g chitin, 20g yeast extract, 50g peptone, 2g KH 2 PO 4 and 0.5g MgSO 4 , sterilized at 121°C for 20 minutes, and set aside;

[0021] (3) Mix (1) sterilized Chizhi spore powder and (2) Cordyceps militaris seed culture solution at a solid-to-liquid mass ratio of 1:1, and ferment at 28°C for 4 days in the dark; solid-liquid separation after ferme...

Embodiment 2

[0023] A method for biological wall breaking of Ganoderma lucidum spore powder, said method comprising the following steps:

[0024] (1) The Chizhi spore powder was sterilized in a Chinese patent medicine sterilizing cabinet, and the sterilization condition was 121° C. for 30 minutes.

[0025] (2) Preparation of Cordyceps militaris seed culture medium: Inoculate the strain of Cordyceps militaris ACCC52244 into the liquid seed culture medium (same as Example 1), culture at 28°C, 150r / min for 2 days, and set aside.

[0026] (3) Mix (2) Cordyceps militaris seed culture solution with (1) sterilized Ganoderma lucidum spore powder at a solid-to-liquid ratio of 1:1.5, and ferment at 25°C for 5 days in the dark; solid-liquid separation after fermentation, and dry , that is, the Ganoderma lucidum spore powder after breaking the wall; the wall breaking rate of the spore powder is about 98.5% as detected by the blood count method.

Embodiment 3

[0028] A method for biological wall breaking of Ganoderma lucidum spore powder, said method comprising the following steps:

[0029] (1) Using C 0 60 irradiation to sterilize Chizhi spore powder, the irradiation dose is 25kGy

[0030] (2) Preparation of Cordyceps militaris seed culture solution: Inoculate the strain of Cordyceps militaris ACCC52244 into the liquid seed culture solution (same as Example 1), culture at 25°C, 150r / min for 3 days, and set aside.

[0031] (3) Mix (2) Cordyceps militaris seed culture solution with (1) sterilized Chizhi spore powder at a solid-to-liquid mass ratio of 1:2, and ferment at 18°C ​​for 6 days in the dark; solid-liquid separation after fermentation, After drying, the Ganoderma lucidum spore powder after breaking the wall can be obtained; the wall breaking rate of the spore powder is about 99.3% as detected by the blood count method.

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Abstract

The invention provides a biological wall-breaking method of aseptic ganoderma lucidium spore powder. The method comprises: adding cordyceps militaris into the aseptic ganoderma lucidium spore powder for solid fermentation, and making the ganoderma lucidium spore powder to break the wall. By using the chitinase-secreting cordyceps militaris as a wall-degrading fungus, effective and safe wall breaking for ganoderma lucidium spore can be carried out, and thereby the active substances of the ganoderma lucidium powder are furthest reserved and protected, and are in favor of human body absorption and utilization; and not only the cordyceps militaris fermentation process makes ganoderma lucidium spore to break the wall, but also the cordyceps militaris mycelium does not need removing, because cordyceps militaris is a beneficial microbe, and can generate active substances such as cordyceps polysaccharide, cordycepic acid, cordycepin and the like which can cooperatively reinforce the efficacies of the aseptic ganoderma lucidium spore powder.

Description

(1) Technical field [0001] The invention relates to a biological wall breaking method of ganoderma lucidum spore powder. (2) Background technology [0002] Ganoderma lucidum is a traditional Chinese food and medicine dual-purpose precious medicinal material, which has the functions of strengthening the body and strengthening the body, and is listed as the top grade in "Compendium of Materia Medica". Ganoderma lucidum spores are extremely small spores ejected from the cap during the mature growth stage of Ganoderma lucidum, and accumulate a large amount of active ingredients such as polysaccharides, triterpenoids, and ganoderma spore oil. A large number of modern pharmacological studies and clinical cases have proved that Ganoderma lucidum spore powder has a good regulatory effect on the immune system, nervous system, endocrine and metabolic systems, and cardiovascular system. However, Ganoderma lucidum spores have a double-layer outer wall made of chitin, which is extremely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61K9/14
Inventor 吴学谦徐娟吴学良吴庆其陈如楚
Owner ZHEJIANG ESSENCE FUNGI DEV
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