734 results about "Cordycepin" patented technology

The invention aims at providing a health-care food enhancing immune function of human body and provides a preparation technique thereof. The health-care food, according to weight proportion, is produced by raw materials comprising 10-50 portions of gen-seng, 10-50 portions of glossy ganoderma and 10-50 portions of zymotic cordycepin powder; the raw materials comprising the gen-seng, the glossy ganoderma and the zymotic cordycepin powder are positioned in a hop-pocket; the processed raw materials are carried out heating and decoction for 1-3 times (each time is for 1-2 hours) after being added with water for soaking for one hour; extraction solution is mixed, filtered and concentrated to defined amount; after being cooled, concentrated solution is carried out high-speed centrifugal impurity removal; the obtained concentrated solution is produced into required preparation independently or by adding with accessories acceptable in medical science with the conventional processes of pharmacy.

The invention provides a method for producing cordyceps militaris fermentation rice (cordyceps rice for short) by utilizing solid state fermentation of rice, and the method is characterized in that the rice is taken as a main fermentation substrate; and the cordyceps mycelia grows in the rice through a solid state fermentation manner so as to obtain the rice rich in the cordyceps mycelia. The method comprises the following steps: 1. preparing a cordyceps militaris liquid strain; 2. preparing a rice solid state fermentation culture medium; and 3. inoculating and fermenting. In the method, the medical effect of the cordyceps militaris is organically combined with the nutrition of the rice, thus the product contains various nutritive components and mineral elements, especially cordycepin andcordycepic acid, wherein, the content of the cordycepin is more than or equal to 600mg / 100g, and the content of the cordycepic acid is more than or equal to 3g / 100g, thereby improving the immunity ofthe organism, inhibiting tumours, preventing ageing, and satisfying the requirements of healthy eating of people.

The present invention relates to a method for extracting the purificatory cordycepin and the cordyceps amylase from the cordyceps militaris, belonging to the fields of medicines and health protection and chemical engineering. The cordyceps militaris powder is extracted and filtrated by a cooling seep method, the filter residue is mixed with ethanol, the supersonic wave assists to extract and offcenter, the supernatant has the processes of concentration and alcohol-precipitating, then the alcohol-precipitated supernatant has the processes of decompression, concentration and elution, the eluted liquid has the processes of concentration and crystallization and recrystallization using the n-butyl alcohol, at last the purificatory amylase is obtained after freezing and drying. The preparation progress of the present invention only uses water and ethanol as the solvent, thus reducing the pollution, the resin is capable of being reused a plurality of times with low cost. The present invention fully exploits the biological activity ingredients of the cordyceps militaris, realizes the coextraction of the cordycepin and the cordyceps amylase, guarantees the biological activities of the cordycepin and the cordyceps amylase, farthest increases the utilization rate of the raw material to the largest extent, reduces the manufacturing cost. The present invention is suitable for the large-scale industrialized manufacture.

The invention discloses a method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation, wherein bacteria strain activation and antagonism test are required beforehand, and afterwards, the cordycepin are required to be detected. The method comprises four stages: (1) red yeast rice cultivation; (2) cordyceps militars cultivation; (3) dixenie mixed synergistic fermentation; and (4) fruiting body management. The method not only can significantly improve the output of the cordyceps militars fruiting body by more than 30 percent, but also can improve the quality of the cordyceps militars fruiting body, thus causing the content of cordycepin in the fruiting body to be improved by 20 percent and reach more than 20.2mg / g. The method has great significance in improving the output and efficiency of cordyceps militars solid fermentation, and improving the content of effective constituents in medical cordycepin powder and capsule, thus having good application and development prospect.

The invention relates to a novel method for breeding cordyceps militaris by utilizing silkworm larvae. The method comprises the steps of taking live matured silkworm as the host, vaccinating cordyceps militaris strains through an injection method, and promoting silkworm to sleep, and the vaccination attainment rate is improved; after the method is adopted, the inactivation rate of the silkworm larvae reaches 90% to 100%, and the outgrowth rate of fruiting body reaches 85% to 100%; moreover, the water-solubility protein, polysaccharide, cordycepic acid, cordycepin and adenosine of the cordyceps militaris bred by the method are higher than those of cordyceps militaris bred by silkworm chrysalis in content. The novel method for breeding cordyceps militaris by utilizing silkworm larvae has the advantages of convenience in operation, low breeding cost, good experiment repeatability and wide application prospect, and is suitable for large-scale cultivation.

This invention describes the large-scale fermentation and processing of Chinese caterpillar fungus. In this invention, hirsutella sinensis strains are used as the fermentation strain and cultivated in the inclined plate and shaking-bottle. 3-5% of potato, 0.5-2% of soybean powders, 0.01-0.02% of soifnum muicfun, 0.01-0.02% of Chinese yam, 0.5-2% of protein, 0.5-5% of glucose, a small amount of dipotassium hydrogen phosphorus and magnesium sulfate, and water culture medium are successively directed into all levels of fermenter for fermentation and processing. The yield of the fermented product is 20% higher than that in the present processes, the fermentation periodic time is 12 days shorter, the cost is 20% lower and the pollution rate is 2% lower. Through the DNA molecular sequence examination by the institute of microbes, Chinese Academy of Sciences, the constitution of the fermented product is the same with that of natural Chinese caterpillar fungus, and the content of cordycepin, adenosine, Chinese caterpillar fungus polyoses, sterol, biological base, amino acids and trace elements is much higher than that in natural Chinese caterpillar fungus. The pharmacological action of the fermented product is better than that of natural Chinese caterpillar fungus.

The invention provides a method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds. The method comprises the following steps: on the basis of utilizing the steamed lotus seeds as a worm grass solid state fermentation culture medium, inoculating 3-8ml worm grass liquid strain to per 100 g of the lotus seeds; culturing for 10-30 days at the temperature of 5-30 DEG C and the humidity of 60-90% in dark, namely obtaining worm grass lotus seeds on which hyphae are overgrown; and culturing the worm grass lotus seeds on which the hyphae are overgrown at the temperature of 5-30 DEG C and the humidity of 80-95% for 10-30 days with the illumination of 8-15 hours per day, thus obtaining the worm grass lotus seeds on which worm grass sporocarps are grown. The invention provides a new worm grass source and a new lotus seed health food. According to the invention, the medical effect of worm grass and the nutrition of the lotus seeds are organically combined, so that the product has various nutrient components, is especially rich in active components such as cordycepin, a cordycepic acid, a worm grass polyose and the like and has high edible and medical values; and the worm grass lotus seed fungus preparation process is simple and can easily realize large-scale production, popularization and implementation.

The invention provides a method for industrialized cultivation of Cordyceps militaris with high content of cordycepin. The method of the present invention uses the screened bacterial strains Cordyceps militaris COB201 and CGMCC No.1823 to carry out optimized cultivation to obtain Cordycepin militaris with a high content of cordycepin. The content of cordycepin in the product reaches 1.7% after testing. The method of the present invention is simple and suitable for large-scale industrialization Production.

The invention discloses river crab feed, which comprises fish meal, vegetable dregs, corn, soya bean meal, inferior meal, soya-bean oil, shrimps, cuttlefish ointment, monocalcium phosphate, mineral substances for crabs, vitamin for the crabs, a binder, 0.7 to 14.0 mg / kg of cordycepin, 131.0 to 2,620.0 mg / kg of cordycepic acid, 0.1 to 2.0 mg / kg of adenosine and 290.0 to 5,800.0 IU / kg of superoxide dismutase. The river crab feed has the high utilization rate, reduces the pollution of feed residues to environment, avoids the negative effects of antibiotic residues and tolerance caused by the antibiotic residues, is safe and harmless to the craps and the environments and can enhance the immunity of the crabs and accelerate the growth of the crabs. The invention also provides a production process of the river crab feed, which has the characteristics of low cost and simple and convenient operation.

The invention belongs to the technical field of health wine, and discloses a preparation method of cordyceps wine which has health care effect for human health. The preparation method comprises the following steps of: taking grain and cordyceps substrates according to the proportion of 1:0.8-1.2; crashing; adding water; steaming for sterilization; putting into a cylinder for ferment; separating wine and lees; and slaking to produce the cordyceps wine. In the preparation process, the cordyceps substrates and the grain are adopted as raw materials, the effective components of cordycepin, cordyceps polysaccharide, and the like in the substrates are dissolved into the prepared cordyceps wine. The cordyceps wine prepared by the process has rich raw materials, changes waste into valuable and can greatly reduce the production cost of the cordyceps wine, otherwise, the cordyceps wine can be produced by a solid fermentation process, the effective components of cordycepin, cordyceps polysaccharide, and the like in the cordyceps substrates can be better dissolved into the wine, and the cordyceps wine can generate special flavor and is beneficial to increasing the function and the quality of nutrient wine.

The invention provides an extraction and purification method of cordycepin from Cordyceps militaris, and belongs to the deep-processing technical field of agricultural products. The method comprises the following steps: crushing Cordyceps militaris raw material, mixing the raw material with water at 1:20 raw material / water ratio, performing ultrasonic extraction, and performing centrifugal filtration on obtained extracting solution and pouring in a macroporous resin column at the adsorption flow of 1.8BV / hr; taking 15% methanol aqueous solution as an eluant to perform resin column elution, and decompressing and condensing obtained eluent at the temperature of 60 DEG C by a rotary evaporator to obtain Cordyceps militaris cordycepin concentrate. The method can help realize aqueous solution extraction of the cordycepin from the Cordyceps militaris raw material at low temperature, and the extraction rate reaches 85%; and polysaccharide and protein can be removed from the extract by macroporous resin separation and purification, thus the content of the cordycepin in the extract can reach 10%. The cordycepin in the Cordyceps militaris product has high activity, can be taken as a raw material for producing cordycepin monomer and can also be directly taken as an additive of health products and functional food, thus the cordycepin has wide market development prospect.

The invention discloses cordyceps mycelia and a preparation method thereof. The preparation method of the cordyceps mycelia comprises the following steps of inoculation and constant-temperature cultivation of paecilomyces hepialid, seeding tank cultivation, fermentation tank cultivation, ultralow-temperature vacuum concentration, freeze drying and crushing. In comparison with the cordyceps mycelia produced in the prior art, the cordyceps mycelia produced by the method has the advantages that the calcium content is increased by 90-120 times, the magnesium content is increased by 1-2 times, the contents of ferrum, zinc, cordycepic acid and cordycepin are increased by 20-50%, and the superoxide dismutase (SOD) activity is increased by 20-30%. Therefore, the health efficacy of the product is remarkably improved.

A method for artificially culturing north cordyceps to increase the contents of its physiological active components (cordyceptic acid, cordycepin and cordyceptic polyose) includes such steps as washing fresh wild north cordyceps, section, regulating pH of culture medium to 6-7, sterilizing, shaping slant, slant culturing at 20-25 deg.C for 7-10 days, regulating pH of liquid culture medium to 6-7,sterilizing, inoculating, culturing, preparing solid culture medium from rice and sorghum grains, inoculating, and culturing at 25-29 deg.C for 40-60 days.

The invention discloses a method for separation and purification of cordycepin, the method adopts the cordyceps militaris solid drying culture media as the raw materials, and uses NKA-II macroporous resin column and C18 column for purification and finally uses the distilled water for repeated recrystallization. The purity of the obtained cordycepin can achieve more than 95 percent. The method has the advantages of rich sources of the raw materials, low cost, simple process and high purity, so the method is applicable to the industrialization production.

The invention relates to a cordyceps militaris fruit body culture medium, mainly comprising a starch substrate and inorganic salt containing phosphorus, potassium and magnesium ions. The cordyceps militaris fruit body culture medium is characterized by further comprising cordyceps fungi powder which is powder containing cordyceps militaris or powder containing cordyceps militaris mycelium. The invention also provides a preparation method of the cordyceps militaris fruit body culture medium. The raw material of zhao cordyceps fungi powder as the important components of the culture medium is cultured waste material, namely culture medium after the cordycepin is cultured or waste material of the fruit body, and therefore, the production cost is reduced. The cordyceps militaris fruit body cultured by using the culture medium, the grown fruit body has excellent quality, and the content of adenosine and cordycepin as effective components is obviously improved compared with the common culturemedium.

The invention pertains to the field of agriculture microbial technology, in particular to a method for producing cordycepin, a breeding and an application of a high-yield Cordyceps militaris bacterial strain. The method obtains the high-yield Cordyceps militaris bacterial strain BYB-08 by mutation breeding, and the bacterial strain is collected in China Center for Type Culture Collection as a patent procedure before the application date (the collection number is CCTCC NO: M207091). The invention discloses a successful embodiment in a combined method of the optimized deep fermentation and the still placement surface layer fermentation culture of the Cordyceps militaris bacterial strain BYB-08 which is obtained by the method, compared with the cordycepin biological yield which is obtained by the methods which are reported in the existing patent documents and the non-patent documents, the cordycepin content in the obtained fermentation liquid of the invention is significantly improved.

The invention relates to a method for extracting cordycepin and cordyceps polysaccharide from cordyceps militaris. The method comprises the following steps of: water extraction, alcohol extraction, column extraction of supernate and column extraction of sediment. In the water extraction step, the water temperature is set between 55-58DEG C, namely active ingredients in cordyceps can be fully dissolved, and the cordycepin can be prevented from being decomposed. Therefore, resource waste is avoided when the cordycepin and the cordyceps polysaccharide are extracted at the water temperature.

The invention provides a fermentation method for high cordyceps militaris biomass and high cordycepin content, which belongs to the technical field of bio-engineering. A cordyceps militaris strain is activated, inoculated to a first stage seed tank and further expanded to a fermentation tank, when a certain biomass is achieved in the fermentation tank, a biotic elicitor of Xylaria type endophytic fungus from binkgo biloba extract liquid and non-biotic elicitors of sodium acetate and ammonium sulfate are added for promoting the production of cordycepin in bacteria. The fermentation method adopts an optimized culture medium for effectively increasing the mycelial growth of cordyceps militaris; furthermore, under the regulation role of the elicitors, the cordyceps militaris biomass and the cordycepin content in the bacteria are respectively increased by 1.76 times and 7.14 times compared with the contrast culture medium without the addition of the elicitors and respectively increased by 2.35 times and 23.25 times compared with the ordinary improved PDA. The fermentation method greatly improves the production amount of cordyceps militaris mycelia and the content of the active characteristic ingredient, thereby having broad market prospect.

The invention relates to a method for extracting a composition containing nano-oligopeptide from cordyceps militaris as well as the composition extracted by the method. In the method, the cordyceps militaris is hydrolyzed by a complex enzymatic method to obtain the small molecular nano-oligopeptide. The invention is characterized in that the molecular weight of the nano-oligopeptide is mainly distributed within 100-1000D; and preferably the Gaussian distribution peak area percentage of the nano-oligopeptide the molecular weight of which is 100-500 D in the composition is more than 50%. The cordyceps militaris oligopeptide extract provided by the invention reserves other active ingredients such as cordycepin, ecdysone, adenosine, cordyceps polysaccharide, cordycepic acid, trace elements, vitamins and free amino acid. The invention further relates to an application of the composition containing the nano-oligopeptide extracted from the cordyceps militaris in preparing medicines, healthcare products and cosmetics.

The invention discloses a method for simultaneously extracting cordycepin and cordyceps polysaccharides from a residue of a cordyceps militaris culture medium. The method comprises the following steps of: extracting cordycepin and cordyceps polysaccharides in a residue of a cordyceps militaris culture medium by utilizing an extractant, meanwhile, adsorbing the extracting solution through macroporous resin, and then extracting and adsorbing the residue of the cordyceps militaris culture medium; eluting the cordycepin and the cordyceps polysaccharides, which are adsorbed on the macroporous resin; and concentrating and drying the eluent to obtain a product containing the cordycepin and the cordyceps polysaccharides. The method can simultaneously extract the cordycepin and the cordyceps polysaccharides from the residue of the cordyceps militaris culture medium and has high active constituent content; the resin does not need acid-alkali regeneration and can be recycled; and the method has the advantages of environmental protection, low cost, simple process and equipment and realization of continuous extraction.

The invention belongs to the technical field of fungus culture, particularly to a method for culturing mycelia and sporocarps of aweto, which solves the problem that artificial aweto in the prior art can not be cultured in a large scale. The method comprises the following steps of: selecting cephalosporium acremonium and Isaria cicadae; preparing a cephalosporium acremonium liquid strain and an Isaria cicadae liquid strain; preparing a solid culture medium from corn grit, soybean flour, wheat grains, sorghum grains, silkworm chrysalis meal, whole chicken eggs, black bean coarse powder containing hulls, degreased linseed oil and a nutrient solution, wherein the nutrient solution comprises chicken blood, pine needles, carrots, black beans and cattle bone; evenly mixing, and inoculating; and culturing to obtain the sporocarps and the mycelia. The detection indicates that the sporocarps contain at least 0.02% of adenosine and at least 0.1% of cordycepin, and the mycelia contain at least 0.055% of adenosine; the finger-print test result indicates that the degree of similarity of the sporophores and the mycelia is greater than 97%; and acute toxicity experiments and long-term toxicity experiments indicate that the sporophores and the mycelia have no toxicity and can be used as substitutes of natural aweto.

The invention provides a method for increasing cordycepin content in Chinese caterpillar fungus solid medium, which comprises three steps of activation of fungus, preparation of liquid fungus and culture of the Chinese caterpillar fungus. The method is characterized in that in the method, rice with the grain size of 20 meshes and 14 meshes is mixed according to the mass ratio of 1:2 to obtain culture medium; 3 percent of glucose, 3.5 percent of peptone, 0.05 percent of K2HPO4, 0.05 percent of MgSO4.7H2O, 2mg / L VB1 and 1,000mL water are used to prepare nutrient solution, and the Chinese caterpillar fungus is cultured according to the prior solid fermentation method; and after stroma of the Chinese caterpillar fungus grows out, illumination intensity and time are increased. The method has the advantages of greatly increasing the cordycepin content (more than 0.76 percent) in the culture medium and having important significance on improving yield and efficiency of producing the cordycepin by solid fermentation of the Chinese caterpillar fungus, along with simple and quick operation; therefore, the method is suitable for mass production of the cordycepin by the solid fermentation of the Chinese caterpillar fungus.

The invention disclosed a way to successively extract active ingredients from carpohole of north aweto. The invention adopts super critical CO2 to extract sterin substances, precipitating with alcohol to get nucleoside substances with cordycepin and Cordyceps polyose, separating with normal phase low pressure gel silica chromatography to get crude cordycipin, preparative reversed-phase high-performance liquid chromatography to get cordycepin crystal with high purity. The invention can not only successively extract sterin substances and Cordyceps polyose (CM-1) from the carpohole of north aweto, it can also get the cordycepin metamer; it is not applicable to small experiments but also applicable to industrial production. Since the producers can extract many substances by one treatment of carpohole, the invention can not only decrease the industrial cost but also make the biggest economic returns.

The invention belongs to the technical field of fungus cultivation, and provides a method for cultivating cordyceps militaris. The method can improve the content of cordycepin in the cordyceps militaris and the content of cordycepin is stable around 1.7-2.5%. The method comprises the steps of preparing cordyceps militaris strains, preparing liquid cordyceps militaris strains, and cultivating cordyceps militaris. The cordyceps militaris is cultivated in a cultivation base. Wheat, oat and rice are used as cultivation base main materials, and peptone, silkworm chrysalis meal, sugar, magnesium sulfate and monopotassium phosphate are used as auxiliary materials, all materials are made into solutions to form the cultivation base. Cordyceps militaris sporocarps are obtained through cultivation under a certain cultivation condition. The content of cordycepin in the obtained cordyceps militaris sporocarps is as high as 1.7-2.5% and stable around 2%. Selection of the cordyceps militaris strains is random, 1-2 generations of mother strains of the cordyceps militaris strains can be selected at random, no matter the cordyceps militaris strains are obtained by separation finished by a user himself or bought by the user at different places or stored strains, all the trains can be used.

A solid culture method for northern cordyceps features that its solid culture medium is prepared from glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, triammonium citrate, VB, long-grained non-glutinous rice, and silkworm powder. Its advantage is high content of cordycepin in its sporophores.

The invention provides a method for culturing cordyceps militaris sporocarp with high-content bioactive substances, comprising the following steps: inoculating good cordyceps militaris strains after multiple repeated filtering on a culture medium with the best optimized proportion of carbon to nitrogen sources; and optimizing and culturing at different temperatures, humidity and illumination intensities at different growth periods. Through the cordyceps militaris sporocarp planted and obtained by the method provided by the invention, the contents of bioactive substances are greatly improved, and the bioactive substances mainly comprise the chemical index components of adenosine, cordycepin, cordyceps polysaccharide, cordycepic acid, SOD (Super Oxide Dlsmutase) and the like; for the cordyceps militaris sporocarp planted and obtained by the method provided by the invention, the pharmacology and drug effect are extremely similar to natural Chinese caterpillar fungus, and the contents of bioactive substances of adenosine, cordycepin, cordyceps polysaccharide and cordycepic acid are over that of the natural Chinese caterpillar fungus.

The invention provides a comprehensive method of extracting various effective components from cordyceps militaris. The dry and crushed cordyceps militaris is extracted and filtered with organic solvent to get filtrate and leftover; the filtrate is condensed to recycling the solvent, and the condensed liquor after being saponified, extracted and dried can present aweto sterols; the leftover is extracted and filtered, and the sediment and supernatant are obtained after condensing, alcohol sinking and centrifugating the filtrate; cordycepin polysaccharide is obtained through operations such as dissolving the sediment into water and removing the albumen; the supernatant is processed by methods such as ion-exchange resin column and crystallization to obtain cordycepin. The method is material saving, with high purity of the extracts, low cost and simple operation, thus realizing the comprehensive extraction of various effective components from cordyceps militaris.

Pharmaceutical prodrug compositions are provided comprising azide derivatives of drugs which are capable of being converted to the drug in vivo. Azide derivatives of drugs having amine, ketone and hydroxy substituents are converted in vivo to the corresponding drugs, increasing the half-life of the drugs. In addition azide prodrugs are often better able to penetrate the blood-brain barrier than the corresponding drugs. Especially useful are azide derivatives of cordycepin, 2′-F-ara-ddI, AraA, acyclovir, penciclovir and related drugs. Useful azide prodrugs are azide derivatives of therapeutic alicyclic amines, ketones, and hydroxy-substituted compounds, including aralkyl, heterocyclic aralkyl, and cyclic aliphatic compounds, where the amine or oxygen moiety is on the ring, or where the amine or oxygen moiety is on an aliphatic side chain, as well as therapeutic purines and pyrimidines, nucleoside analogs and phosphorylated nucleoside analogs.

The invention relates to a new preparation technology for preparing adenosine, cordycepin and N6-(2-hydroxyethyl)adenosine chemical reference substances simultaneously which are three chemical reference substances. The preparation technology is that the two-step efficient purification of Cordyceps militaris extract is performed to obtain the three chemical reference substances of which purities are more than 98%, wherein the two-step efficient purification contains resin column separation and high performance liquid chromatography. The preparation technology has simple process steps and high purity and is easy to perform large-scale production.

The invention relates to a Chinese caterpillar fungus polypeptide product that uses silkworm pupa or egg as culture medium, accesses pupa Chinese caterpillar fungus as raw material to gain after degreasing and enzyme hydrolyzing. The invention combines pupa Chinese caterpillar fungus with high protein material. Thus, it contains active ingredients like polypeptide, amino acid, cordycepin, etc. The invention has great edible value and medical value. It has advantages of simple manufacturing technology, short producing cycle, low process cost and it could be used in medicine, edible, and so on.