51 results about "Cordycepin" patented technology

The invention relates to the technical field of mycelium culture, and provides a culture method and application of cordyceps militaris mycelium with high selenium content and high cordycepin content. The culture method comprises the following steps: inoculating cordyceps militaris mycelia into a liquid culture medium, and carrying out shake culture for 4-7 days to obtain cordyceps militaris liquid seeds; adding a plant selenium source into the solid culture medium, sterilizing to obtain a cordyceps militaris selenium-rich solid culture medium, adding the cordyceps militaris liquid seeds into the cordyceps militaris selenium-rich solid culture medium, and continuously culturing for 30-40 days to obtain the high-selenium and high-cordycepin cordyceps militaris mycelium. According to the culture method, the steps are simple and convenient, the raw materials are cheap and safe, the total selenium content of the produced mycelium is 0.76-191.45 mg / kg, the organic selenium content is more than 99%, the cordycepin content is 17707.3-24769 mg / kg, the content of various effective components such as the total selenium, the organic selenium and the cordycepin is outstanding, the yield is high, standardized popularization and application of the selenium industry in multiple fields are facilitated, and the wide market prospect is achieved.

This invention discloses a protein-polysaccharide ternary nanoparticle loaded with cordycepin, its preparation method, and its applications. The invention employs a layer-by-layer self-assembly method to modify the surface of zein nanoparticles. Since zein has a positive surface charge, the first layer modification uses the anionic polysaccharide sodium alginate for electrostatic bonding, and the second layer modification uses the cationic polysaccharide chitosan, constructing positively charged ternary composite nanoparticles. These nanoparticles have small particle size, high encapsulation efficiency, high drug loading capacity, and a sustained-release effect, significantly reducing cytotoxicity and significantly improving the therapeutic effect on osteoarthritis. This invention develops a novel cordycepin formulation, overcoming the limitations of cordycepin's clinical use.

The invention discloses an immunomodulatory composition based on a cordycepin-selenium nano chelate and a preparation method thereof, and belongs to the technical field of biological medicines.The immunomodulatory composition is characterized in that 15-25 nm zero-valent selenium nano particles are chelated through hydroxyl of cordycepin and N7 site bidentate coordination, and a stable five-membered ring structure with the molar ratio is formed; the preparation method comprises the following steps: reducing sodium selenite in an inert atmosphere to generate selenium colloid, chelating the selenium colloid with a cordycepin ethanol solution, and carrying out ultrafiltration and freeze-drying to obtain the product. The invention aims to solve the problems of fuzzy structure, inconsistent batch, uncontrollable immunomodulatory function and the like caused by non-specific mixing in the prior art, and the composition can bidirectionally regulate and control IRF3 pathways, promote polarization of M2 type macrophages and proliferation of Treg cells, and has a clear immunomodulatory mechanism and excellent stability.

The invention discloses a cordyceps sinensis culture control method and system, and core culture targets are set in generations by identifying the subculture generations G1, G2 and G3 of cordyceps sinensis strains: the G1 generation activates a cordycepin and adenosine synthesis pathway, the G2 generation maximizes the total nucleoside content and antioxidant activity, and the G3 generation improves the biomass and delays degradation. The system monitors environmental parameters such as temperature and humidity, illumination, gas concentration and the like, and thallus physiological indexes such as biomass, metabolism precursor concentration, active substance content and the like in real time, and adopts algorithms such as fuzzy PID, NSGA-II multi-objective optimization, model prediction control and the like to dynamically adjust culture conditions such as nutrition supply, dissolved oxygen, illumination period and the like. The method provided by the invention solves the problem that traditional static regulation neglects intergenerational difference of strains, realizes subgeneration refined targeted regulation, remarkably improves the yield and quality of target products, prolongs the service cycle of the strains, and is suitable for industrial bionic cultivation of cordyceps sinensis.

The application discloses a high-quality and high-yield cultivation method of wasp pupa cordyceps, and belongs to the field of artificial cultivation technology of rare medicinal and edible fungi. The method selects frozen wasps at each day age of mature larva stage to metamorphic stage as main raw materials of culture medium, and the culture medium is comprehensive in nutrition and high in quality. The cordyceps cultivated by the method contains high medicinal and nutritional components such as cordycepin, amino acid and cordyceps polysaccharide, and the content of cordycepin is particularly outstanding. The application has high safety and is not affected by environmental factors. The application adopts a pipette injection method, and the culture medium is prepared through normal pressure sterilization or high pressure sterilization. The number of wasp pupae inoculated per hour is 20 times that of the prior art, and the bacterial contamination rate can reach 100%. The application solves the problems of labor-intensive, time-consuming and low bacterial contamination rate of the current manual injection method, shortens the time for the insect body to be fully covered with bacteria by more than one week, and increases the yield by more than 3 times. The application is convenient for large-scale production, and has novelty, creativity and wide application value.

This invention belongs to the fields of genetic engineering and microbial technology, specifically relating to a yeast quorum sensing dynamic regulation system, method, and its applications. This invention successfully constructs a functional, bacterial-derived RpaI / RpaR quorum sensing system in yeast for the first time. The constructed yeast quorum sensing dynamic regulation system possesses advantages such as low background leakage, high sensitivity, and wide dynamic range. By flexibly coupling the core system with a signal amplification module (Gal4) and a signal conversion module (CRISPRi), a bifunctional cascade platform integrating activation and inhibition is constructed, achieving multidimensional and precise dynamic regulation of metabolic pathways. Furthermore, the system has been applied to the biosynthesis of several important compounds, including cordycepin, geraniol, and 3-hydroxypropionic acid, significantly increasing yields and effectively balancing the conflict between cell growth and product synthesis, demonstrating its great potential as a universal dynamic regulation tool.

Provided is a culture medium for enhancing the content of cordycepin from Cordyceps militaris, wherein the culture medium comprises Hermetia illucens adult insects. According to the present invention, the culture medium can ensure a significantly higher cordycepin yield than conventional grain-based media or pupa-based media by means of a method for extracting cordycepin from Cordyceps militaris cultured using Hermetia illucens adult insects as a host.

The present invention relates to a method for producing a fermented powder of Cordyceps militaris with enhanced active ingredients by culturing Cordyceps militaris mycelium using sword beans and brown rice as culture media, and extracting and pulverizing the mycelium. Specifically, the method comprises the steps of: (1) preparing a sterile culture medium by mixing pre-treated sword beans and brown rice in a certain ratio; (2) inoculating the Cordyceps militaris mycelium with accession number KCCM13296P into the culture medium and performing dark culture and photoculture; (3) preparing a complex fermented product by sterilizing, drying, and grinding the culture; and (4) preparing a final powder by adding ethanol to the fermented product to extract active ingredients, and concentrating and freeze-drying the extract. The fermented powder produced in this manner has increased content of beneficial components such as β-glucan, ergosterol, cordycepin, and adenosine, and exhibits excellent inhibitory effects on NO and inflammatory cytokines, making it useful as a raw material for health functional foods or pharmaceuticals.

1. The name of the design product: Cordyceps freeze-dried goat milk powder packaging box. 2. The use of the design product: for packaging Cordyceps freeze-dried goat milk powder. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: front view.

The present disclosure is in the field of biomedicine and particularly relates to a therapeutic agent for the treatment of mitochondrial disorder and promoting mitochondrial function in a subject. The method comprises administering nucleoside derivative cordycepin CO1, its derivatives and a pharmaceutically acceptable carrier to a subject. Also provided is cordycepin, its derivatives and a pharmaceutical composition thereof.

The application discloses a 3'-deoxyadenosine compound and application thereof in preparation of an antitumor drug. The 3'-deoxyadenosine compound and the pharmaceutical composition thereof have good antitumor proliferation effect. Compared with a parent drug, the 3'-deoxyadenosine compound has better affinity to a cell membrane, so that the half-life of in-vivo metabolism of the drug is longer, and the drug stays in the body for a longer time. Compared with other nucleoside antitumor drugs, the cordycepin derivative and the pharmaceutical composition thereof have a wider range of tumor types and effects, including excellent inhibition effect on gastric cancer, pancreatic cancer, liver cancer, small cell lung cancer, colorectal cancer, melanoma, ovarian cancer and the like, and the side effect is lower and the curative effect is better.

The present application provides a kind of Cordyceps militaris pre-made noodles and its preparation method, belong to food processing technical field especially.The present application is prepared by compounding wheat flour, Cordyceps militaris fruiting body ultrafine powder, Cordyceps militaris fermented wheat flour and Cordyceps militaris fruiting body enzymatic solution, supplemented with konjac flour, gluten powder, a kind of pre-made noodles with Cordyceps militaris flavor and nutritional activity rich in cordycepin, cordyceps polysaccharide, cordyceps acid, adenosine, flavone and vitamin.The pre-made noodles of the present application are smooth in taste, rich in functional components, enrich the market product variety of Cordyceps militaris, solve the existing Cordyceps militaris noodle poor toughness, easy to break during cooking process, easy to paste soup, taste and taste is not good, Cordyceps militaris addition is less, actual nutritional effect is insufficient, and cooking process nutrition is easy to lose, etc.Problems, realize the high value utilization of Cordyceps militaris, promote the application range of Cordyceps militaris, bring the Cordyceps militaris delicious and nutritious to more consumers.

The application discloses a kind of scalable production's bee pupa cordyceps cultivation method, belong to precious edible mushroom artificial cultivation technical field, select frozen honeybee in mature larva period to metamorphosis period each day old pupa body as culture medium main raw material, through normal pressure sterilization or high pressure sterilization production culture medium, using pipette gun injection method, such as every hour inoculation amount is 20 times of prior art, bee pupa cordyceps culture medium dyeing bacteria success rate can reach more than 98%, such as solve the current by hand injection method inoculation speed slow, time-consuming, dyeing bacteria rate poor drawbacks, and dyeing bacteria period time can be shortened by more than one week, bee pupa cordyceps single bottle yield is more than 2 times of prior art, high safety performance, it is favorable to scale production.The bee pupa cordyceps active ingredient cordycepin content is very high, medicinal value is big, development prospect is wide, not only can drive more beekeeping person to make money, also opens another piece of sky of precious medicinal and edible mushroom industrial production.

The invention discloses a pharmaceutical composition for adjuvant therapy of nodular diseases and a preparation method thereof, and relates to the technical field of medicines.The pharmaceutical composition is prepared from, by weight, 1-10 parts of cordycepin, 10-20 parts of spica prunellae extract, 10-30 parts of plant extract for softening hardness to dissipate stagnation and 15-30 parts of functional auxiliary materials; the cordyceps nucleoside is a nucleoside component of which the purity is greater than or equal to 98%; the mass ratio of oleanolic acid to ursolic acid in the prunella vulgaris extract is 3: 1, and the total content of oleanolic acid and ursolic acid is greater than or equal to 12%; the hard lump resolving plant extract is an extract of thunberg fritillary bulb, radix scrophulariae, oyster powder, seaweed, kelp, radix curcumae and radix bupleuri; the functional auxiliary material is formed by compounding at least three of a filling agent, a disintegrating agent, a suspending aid and an adhesive according to an equal mass ratio; the multiple raw materials are scientifically proportioned to play a synergistic role, the preparation process is standardized, active ingredients are reserved, indexes of finished products are stable, and large-scale production and adjuvant therapy of the nodular diseases can be achieved.

The application discloses a method for preparing functional caragana fiber compound feed and belongs to the technical field of feed processing. The method comprises the following steps: taking caragana as raw material, performing solid-liquid separation after dilute sulfuric acid pretreatment, preparing cordycepin and lysine through liquid state fermentation of cordyceps militaris and glutamic acid corynebacterium respectively, neutralizing caragana solid fiber and caragana fiber raw material obtained through ammonia treatment, and preparing fiber feed through solid anaerobic fermentation of the compound fermentation strain, and finally, compounding the three to obtain functional caragana fiber compound feed. The method realizes efficient utilization of all components of caragana sugar and fiber resources, and the prepared compound feed is rich in cordycepin, lysine and dietary fiber, the crude protein content of the fiber feed is more than 20%, and the fiber feed is rich in cordycepin and lysine functional components. The method not only provides a new way for resource utilization and high value utilization of caragana, but also provides a new idea for research and development of functional coarse feed, and has good economic, ecological and industrial popularization value.

The application discloses a Leuconostoc strain and application thereof, and the Leuconostoc strain HX-236 is screened by the inventors. The Leuconostoc strain HX-236 uses cordyceps militaris powder as the only nitrogen source, and after fermentation culture, a fermentation filtrate can be obtained. The fermentation filtrate has high contents of cordycepin and cordycepic acid, is easily soluble in water, is convenient to use, has good stability, has good compatibility, can significantly improve the expression of hypoxia inducible factor (HIF-1 alpha) in cells, thereby activating downstream signal pathways for regulating inflammatory reactions, cell autophagy and tissue neogenesis, and then regulating cell hypoxia pressure, improving problems of skin aging such as cell activity reduction, collagen loss, pigmentation and skin elasticity reduction caused by hypoxia, and having wide application prospects in the fields of medicines, cosmetics and the like.

The application discloses a compound with anticancer effect based on 3'-deoxyadenosine modified by chemical modification, and a structure of the compound is shown as formula I. The cordycepin derivative and the pharmaceutical composition thereof have good antitumor proliferation effect. Compared with the parent drug, the cordycepin derivative has better affinity to the cell membrane, so that the half-life of the drug metabolism in the body is longer, and the drug stays in the body for a longer time. Compared with other nucleoside antitumor drugs, the cordycepin derivative and the pharmaceutical composition thereof have a wider range of tumor types and effects, including excellent inhibition effect on gastric cancer, pancreatic cancer, liver cancer, small cell lung cancer, colorectal cancer, melanoma, ovarian cancer and the like, and the side effect is lower and the curative effect is better.

The invention discloses a preparation method of a cordyceps militaris fermentation filtrate, the cordyceps militaris fermentation filtrate and application, the preparation method comprises the following steps: mixing a cordyceps militaris strain with a liquid culture medium, dispersing and culturing to obtain a high-density cordyceps militaris strain seed solution; inoculating the high-density cordyceps militaris seed solution into a fermentation culture medium for fermentation, adding Tween 80 in the middle and later periods of the logarithmic growth phase of microorganisms in the fermentation process, adding sophorolipid in the stable growth phase of the microorganisms, and obtaining a mixed fermentation solution after the fermentation is finished; and carrying out inactivation, centrifugation, freeze thawing, filtration, re-inactivation and preservative treatment on the mixed fermentation liquid to obtain the cordyceps militaris fermentation filtrate. The cordyceps militaris fermentation filtrate prepared by the method disclosed by the invention is high in cordycepin content, strong in oxidation resistance and relatively high in stability, and has a very good application prospect in cosmetics, health care products or raw material medicines.

The application discloses a cordycepin-V type starch inclusion compound and a preparation method thereof, wherein the cordycepin is used as a guest to be included, and the V type starch is used as a carrier, and the relative crystallinity of the V type starch is 11.07-25.12%. In the preparation method, the V type starch is prepared by using a glycerol ethanol hot substitution method, and then the cordycepin-V type starch inclusion compound is prepared by weighing the cordycepin and the V type starch according to a mass ratio of 1:80. The prepared inclusion compound can improve the stability of the cordycepin in gastric juice and prolong the intestinal release time of the cordycepin under the premise of ensuring the loading amount of the cordycepin, and provides a new thought and theoretical guidance for effectively improving the bioavailability of the cordycepin.

The present application relates to a kind of cordyceps militaris extract and its extraction process, belong to traditional Chinese medicine extraction technical field.The present application is through enzymolysis, pulse ultrasonic and supercritical extraction synergistic effect to improve extraction efficiency.Specifically including: cordyceps militaris raw materials and cellulase, pectinase mixed enzymolysis, under the protection of inert gas, adding the protective agent containing glutathione and ascorbic acid and ethanol-citrate buffer solution is treated by pulse ultrasonic;Extract liquid is concentrated after supercritical CO2 extraction removes fat-soluble impurities, freeze-drying obtains extract.The obtained extract cordycepin content is ≥0.8%, and the mass ratio of cordycepin and cordycepic acid is stably at 1:0.2-1.The process solves the problem of serious damage of active ingredient in traditional method, low efficiency.

The invention relates to the technical field of cordyceps militaris culture, in particular to a culture method for planting cordyceps militaris through silkworms, which comprises the following steps: activating a strain through a solid culture medium, transferring into a liquid culture medium, and carrying out shake culture to obtain a hypha suspension; inoculating the bacterial liquid to a silkworm chrysalis substrate subjected to composite coating pretreatment in a sterile environment, and carrying out dark culture under a first condition of low temperature and light shielding until hyphae overgrow; and then converting to a second condition with day and night temperature difference and scattered light to induce primordium formation, and then adjusting to a third condition with constant temperature and high humidity to promote fruiting bodies to be mature and then harvesting. According to the cultivation technology, pollution can be effectively reduced, growth can be promoted, the yield can be effectively increased, the quality of cordyceps militaris sporocarp can be remarkably improved, and the content of main active ingredients, namely cordycepin and polysaccharide, of cordyceps militaris can reach the high level.

The application belongs to the technical field of biological medicine, and particularly relates to a cordycepin nano delivery body and a preparation method and application thereof. The method utilizes rolling circle amplification technology, and connects the cordycepin on the circular DNA through base complementary pairing to obtain the cordycepin nano delivery body. The prepared cordycepin nano carrier is a nanoparticle, and the particle size is 100-120 nm. The application utilizes the characteristics that the cordycepin can form unconventional base complementary pairing with DNA, and first and successfully adopts the biological macromolecule DNA as the cordycepin carrying carrier to obtain the cordycepin nano delivery body with high efficient carrying, controllable slow release and high biological safety, and the cordycepin nano delivery body has good targeting property to melanoma. The cordycepin nano delivery body has mild and safe preparation condition, and the preparation process is simple and easy to operate, and is beneficial to the industrialized production of the nano carrier and the wide clinical application.

The invention belongs to the technical field of feeds, and particularly relates to a special feed for low-cholesterol eggs and a preparation process of the special feed. The invention discloses a composition. The composition is prepared from the following components: plant polysaccharide, plant polypeptide, hesperidin, lactobionic acid, cordycepin and pubescent holly root saponin. The invention also discloses an application of the composition in preparation of a special feed for low-cholesterol eggs. A certain amount of the composition is added into the basal feed, the composition takes plant polysaccharide and plant polypeptide as main raw materials and takes hesperidin, lactobionic acid and other active molecules as auxiliary materials, and the combination of the components has a synergistic effect, so that the immunity and disease resistance of laying hens can be effectively improved, the laying rate is ensured, and the quality of laying hens is improved. Meanwhile, the content of cholesterol in eggs can be reduced, the color of the eggs is improved, the viscosity of egg white and the strength of eggshells are improved, the breakage rate is reduced, and the comprehensive quality of the eggs is comprehensively improved.

This invention discloses a health-preserving Cordyceps and Polygonatum Soup and its preparation method. The preparation method includes steps such as raw material pretreatment, extraction and construction of selenium-based composite molecules, preparation of selenium-based nanocomposites, preparation of gel microspheres, preparation of soup base and finished product. This invention significantly improves the extraction rate of functional substances such as cordycepin, cordycepic acid, Polygonatum polysaccharides, and Polygonatum saponins by introducing organic selenium elements; it achieves efficient intestinal delivery by constructing targeted nanocomposites using mannose and fructose, combined with chitosan derivative-modified gel microspheres; this product has significant immunomodulatory, anti-fatigue, lung-moistening, qi-tonifying, anti-inflammatory, and lung-protecting functions, as well as improving intestinal flora. The finished product has a thick, smooth texture, a soothing and sweet aftertaste, and achieves natural preservation without the addition of chemical preservatives, providing a highly efficient and safe new ready-to-eat product for the health food industry.

The invention belongs to the technical field of specific therapeutic activity of compounds or pharmaceutical preparations, and particularly relates to a cordycepin nano-liposome, preparation and application of the cordycepin nano-liposome in treatment of lung injury. The cordycepin nano-liposome provided by the invention is composed of cordycepin, lecithin, cholesterol, dipalmitoyl phosphatidylcholine-polyethylene glycol and water-soluble pentosan, is prepared by adopting a film hydration method, and has high drug loading capacity, high bioavailability and excellent physical stability; precise lung targeted delivery is achieved by means of the synergistic effect of the components, and in-vivo experiments prove that the nano-liposome can effectively inhibit inflammatory response of the acute lung injury part and remarkably improve the lung injury symptom, provides an efficient treatment scheme for acute lung injury, and has important clinical transformation value and wide application prospects.

The invention discloses an engineering strain for improving the yield of cordycepin of cordyceps militaris based on polygene combination expression and precursor engineering and a construction method of the engineering strain, a strong promoter gpdA is adopted for driving, a P2A peptide connection strategy is utilized, and two polygene expression modules are constructed: firstly, cordycepin biosynthesis key genes cns1 and cns2 are overexpressed at the same time, and then the cordycepin biosynthesis key genes cns1 and cns2 are overexpressed at the same time; the terminal catalytic efficiency of the synthetic route is enhanced; and 2, the cns1 and a precursor supply gene nt5e are subjected to combined expression, so that the synergistic enhancement of precursor supply and terminal catalysis is realized. And introducing the expression module into cordyceps militaris through an agrobacterium-mediated method to obtain an engineering strain with genetic stability. A fermentation experiment shows that the cordycepin yield of the cns1-P2A-cns2 engineering strain reaches 186.5 mg / L, which is 2.3 times that of a wild type; and the yield of the cns1-P2A-nt5e engineering strain is further increased to 252.6 mg / L, which is 3.1 times that of a wild type. According to the invention, the induction cost is avoided, the construction is simple, the stability is strong, and a new strategy is provided for industrial efficient production of cordycepin.

The invention discloses a brucea javanica extract-cordycepin HPV (human papillomavirus) attenuated preparation as well as a preparation method and application thereof, and particularly relates to the technical field of antiviral preparations and gynecological medical treatment. The buccal tablet is prepared from the following components: 5%-15% of a brucea javanica extract, 0.3%-1.0% of cordycepin, 1%-3% of a surfactant, 2%-5% of a penetration enhancer, 0.5%-2% of a thickening agent, a pH regulator and the balance of purified water, the dosage of the pH regulator is a dosage for stabilizing the pH value of the preparation in a range of 4.0-6.5. Through pharmacological synergy of the brucea javanica extract and the cordycepin, a breakthrough of dual effects of direct virus resistance and active immune regulation is realized, the virus load is rapidly reduced, and the immune microenvironment of HPV persistent infection is fundamentally reversed by enhancing the self-cleaning capability of the body, so that the immune effect of the human body is improved. Through cooperation of double strategies of physical particle size optimization and chemical penetration enhancement, it is ensured that high-activity medicine components can efficiently permeate and be enriched in a focus part, and therefore the exertion speed and the action strength of the medicine effect are remarkably improved.

The invention relates to liquid modified milk and a preparation method thereof, the liquid modified milk comprises animal milk, glucosamine hydrochloride, calcium beta-hydroxy-beta-methylbutyrate and cordyceps militaris extract, the cordyceps militaris extract contains cordycepin and adenosine, the cordycepin content in the liquid modified milk is greater than or equal to 1.6 mg / 100mL, and the adenosine content in the liquid modified milk is greater than or equal to 0.6 mg / 100mL. According to the modified milk provided by the invention, cordycepin enhances the biosynthesis and energy supply capability of mitochondria by activating related signal channels, and provides an energy basis for the GaHMB to play a role, so that the synthesis efficiency of muscle protein is improved; adenosine and glucosamine have a synergistic effect, so that conduction of inflammation-related signals can be blocked, and decomposition of cartilage matrixes is reduced; meanwhile, glucosamine is used as a glycosaminoglycan precursor, under the action of a signal channel regulated and controlled by adenosine, the synthesis efficiency of cartilage tissue can be remarkably stimulated, the utilization efficiency of sugar is improved, and finally triple synergistic intervention of muscle-joint-metabolism is achieved.

The application discloses a cordycepin derivative, a preparation method and application thereof. The structural general formula of the cordycepin derivative is provided in the application. The cordycepin derivative is modified by a special group, so that the stability of the cordycepin derivative is improved, and the biological activities such as antibacterial activity, anti-inflammatory activity, antioxidant activity and antitumor activity are also significantly improved. In addition, the cordycepin derivative in the application has certain selectivity in the aspect of antibacterial activity, especially for some beneficial bacteria, so that the application range of the cordycepin derivative is expanded.

Cordycepin oxalate of formula (I) (C10H13N5O3.H2C2O4) is provided. A method of preparing cordycepin oxalate is also provided comprising the steps: (i) physical mixing: mixing oxalic acid and cordycepi