118 results about "Cordycepin" patented technology

The invention relates to a saccharomyces cerevisiae strain for efficiently synthesizing cordycepin as well as a construction method and application of the saccharomyces cerevisiae strain, and belongs to the technical field of microbial genetic engineering. The construction method provided by the invention comprises the following steps: carrying out multi-copy genome integrated expression on heterologous codon optimized 2 '-carbonyl-3'-deoxyadenosine reductase genes and 3 '-adenosine monophosphate phosphohydrolase genes, so as to increase the dosage of key enzymes; and supplementing the defective gene of the engineering strain to obtain the saccharomyces cerevisiae strain B5U3 with high yield of cordycepin. According to the obtained saccharomyces cerevisiae strain for efficiently synthesizing the cordycepin, the cordycepin yield reaches 2.92 g / L after shake flask fermentation, the cordycepin yield reaches 7.5 g / L after fermentation in a fermentation tank, and a foundation is laid for safer and more reliable industrial production of the cordycepin.

The invention relates to the technical field of microbial genetic engineering, in particular to a method for improving cordycepin synthesized by saccharomyces cerevisiae and saccharomyces cerevisiae engineering bacteria. According to the invention, codon-optimized 2 '-carbonyl-3'-deoxyadenosine reductase gene and 3 '-adenosine monophosphate phosphohydrolase gene from cordyceps militaris are expressed by using plasmids, and then the plasmids are transformed into host cells to obtain the saccharomyces cerevisiae engineering bacteria. And inoculating a seed solution obtained by seed culture of the activated saccharomyces cerevisiae engineering bacteria into a fermentation culture medium containing a metabolic effector, and carrying out fermentation culture to synthesize cordycepin. Metabolic effectors (Cu < 2 + >, Fe < 2 + >, Mg < 2 + >, Zn < 2 + >, citric acid, cysteine, aspartic acid, glycine, VB1 and tea polyphenol) are added into a fermentation culture medium strain, and the variety and concentration of the metabolic effectors are optimized, so that the yield and synthesis efficiency of cordycepin are remarkably improved. By using the combination of the strain obtained by the invention and the optimal effector, the yield of cordycepin in a fermentation tank reaches 2.9 g / L.

The invention relates to the technical field of data processing, in particular to a data processing method for component content detection.The method comprises the steps that a chromatogram of a cordyceps militaris extract is obtained through high performance liquid chromatography, the abscissa of the chromatogram is retention time, and the ordinate of the chromatogram is an absorbance value; and performing baseline correction on the chromatogram by using an ALS algorithm, in the correction process, adjusting the peak sensitivity of the ALS algorithm based on the adaptive weight of each data point in the chromatogram, and performing baseline correction so as to detect the cordycepin concentration in the cordyceps militaris extract based on the chromatogram after baseline correction. According to the method, the baseline correction accuracy of the chromatographic data of the cordyceps militaris extract can be improved, so that accurate detection of the concentration of cordycepin in cordyceps militaris is realized.

The invention provides cytokine hydrogel as well as a preparation method and application thereof in skin injury repair, and belongs to the technical field of biological medicines. Comprising the following steps: (1) adding beta-GP into a chitosan solution to obtain a CS-beta-GP pregel solution; (2) dissolving Pluronic F127 and hyaluronic acid in a PBS (Phosphate Buffer Solution) until the Pluronic F127 and the hyaluronic acid are completely transparent to obtain an F127-HA solution; and (3) sequentially dissolving trehalose, cordycepin, a cell factor composition and collagen peptide in a PBS solution, incubating to obtain a cell factor mixed solution, and mixing the cell factor mixed solution with the CS-beta-GP pregel solution and the F127-HA solution. According to the invention, a dual temperature-sensitive system is established, the gel can be quickly formed under the conditions of injection at low temperature and body temperature, the effective period of active ingredients is prolonged by utilizing a trehalose stabilization technology, and meanwhile, the anti-inflammatory effect of cordycepin and the cell repair effect of collagen peptide are combined, so that the efficient repair of skin injury can be realized.

The invention belongs to the technical field of medicine crystals, and relates to cordycepin-oxalate as well as a preparation method and application thereof. The invention relates to a preparation method of cordycepin-oxalate. The preparation method comprises the following steps: (1) physical mixing: sufficiently and uniformly mixing oxalic acid and cordycepin-crystal form II crystals; (2) liquid auxiliary grinding: adding an auxiliary agent, and continuously grinding to obtain a solid mixture; and (3) drying the solid mixture to obtain the cordycepin-oxalate. According to the method, the cordycepin-oxalic acid eutectic salt is prepared for the first time, the dissolution rate of the cordycepin-oxalic acid eutectic salt is obviously lower than the dissolution rate of cordycepin reported in the prior art, and the cordycepin-oxalic acid eutectic salt has extremely high potential medicinal application value. The cordycepin-oxalic acid eutectic salt has important technical innovation and market application potential due to the slow release effect.

The invention relates to the technical field of mycelium culture, and provides a culture method and application of cordyceps militaris mycelium with high selenium content and high cordycepin content. The culture method comprises the following steps: inoculating cordyceps militaris mycelia into a liquid culture medium, and carrying out shake culture for 4-7 days to obtain cordyceps militaris liquid seeds; adding a plant selenium source into the solid culture medium, sterilizing to obtain a cordyceps militaris selenium-rich solid culture medium, adding the cordyceps militaris liquid seeds into the cordyceps militaris selenium-rich solid culture medium, and continuously culturing for 30-40 days to obtain the high-selenium and high-cordycepin cordyceps militaris mycelium. According to the culture method, the steps are simple and convenient, the raw materials are cheap and safe, the total selenium content of the produced mycelium is 0.76-191.45 mg / kg, the organic selenium content is more than 99%, the cordycepin content is 17707.3-24769 mg / kg, the content of various effective components such as the total selenium, the organic selenium and the cordycepin is outstanding, the yield is high, standardized popularization and application of the selenium industry in multiple fields are facilitated, and the wide market prospect is achieved.

This invention discloses a protein-polysaccharide ternary nanoparticle loaded with cordycepin, its preparation method, and its applications. The invention employs a layer-by-layer self-assembly method to modify the surface of zein nanoparticles. Since zein has a positive surface charge, the first layer modification uses the anionic polysaccharide sodium alginate for electrostatic bonding, and the second layer modification uses the cationic polysaccharide chitosan, constructing positively charged ternary composite nanoparticles. These nanoparticles have small particle size, high encapsulation efficiency, high drug loading capacity, and a sustained-release effect, significantly reducing cytotoxicity and significantly improving the therapeutic effect on osteoarthritis. This invention develops a novel cordycepin formulation, overcoming the limitations of cordycepin's clinical use.

The invention discloses a cordyceps militaris strain with high yield of ergothioneine and application of the cordyceps militaris strain, and belongs to the technical field of microorganisms and fermentation engineering. On the basis of the screened cordyceps militaris strain with high-yield ergothioneine potential, the cordyceps militaris strain is subjected to ultraviolet mutagenesis treatment, and a cordyceps militaris mutant strain which can be stably inherited and has high-yield ergothioneine is obtained through screening. According to the method, the yield of ergothioneine, cordycepin and cordyceps adenosine in the cordyceps militaris strain is synergistically improved on the basis of precursor directional metabolism remodeling by optimizing and screening precursor substances. The extracting solution of the cordyceps militaris strain can improve the survival rate of lactobacillus reuteri at the high temperature of 50 DEG C and has a protection effect on cells, and the active ingredients of the cordyceps militaris strain can provide an antioxidant protection effect to improve the viability of the cells in an extreme environment.

The present application relates to the field of gene editing technology, bioengineering and application of microbial fermentation, and discloses a Pichia pastoris high-efficiency gene editing system, a construction method thereof and application in a high-yield cordycepin engineering strain.The construction of the Pichia pastoris CRISPR-Cas9 gene editing system mainly includes the following steps: the histidine HIS4 nutritional deficiency back complementation is used to realize the resistance-free assembly of KhCas9; the sgRNA-tRNA array and the IIS type restriction endonuclease combination are used to simplify the gene editing operation and realize the simultaneous and high-efficiency editing of multiple genes; and the Brex27 domain is fused with KhCas9 to strengthen the homologous recombination efficiency. By using the gene editing system, through the optimization of methanol assimilation, 3'-AMP and adenosine precursor supply, ATP / NADPH energy supply and the like, the construction of a cordycepin engineering strain without resistance gene residues is realized for the first time, the gene editing efficiency of Pichia pastoris is greatly improved, and a powerful tool is provided for the industrialized production of cordycepin.

The invention discloses paecilomyces hepialid powder and a production process thereof, and belongs to the technical field of microorganisms. The production process comprises the following steps: S01, slant strain preparation; s02, shaking the seed solution in a flask; s03, fermenting in a fermentation tank; s04, performing centrifugal separation; s05, drying is carried out; and S06, crushing and sieving to obtain the paecilomyces hepialid powder. According to the paecilomyces hepialid powder obtained by the invention, the yield is 42 to 55 g / L, and the adenosine content is 13.29 to 14.83 g / kg; the content of cordycepin is 2.14 to 3.08 g / kg. Static-high-temperature fermentation is introduced in the fermentation process, the obtained paecilomyces hepialid powder has the characteristics of high biomass, high adenosine content and high cordycepin content, the problems of scarcity of cordyceps sinensis resources and high medicine price can be solved, the contradiction between supply and demand can be relieved, the environment can be protected, the cost of the cordyceps sinensis preparation can be reduced, and the application prospect is wide. And the effect of a medication group of the cordyceps sinensis preparation as a tonic drug is increased.

The invention provides a pulmonary nodule targeting food and medicine homologous composition based on metabonomics screening and application thereof. By analyzing a pulmonary nodule specific metabolic profile, active components, namely platycodin D, mogroside V, oyster active peptide and fermented cordycepin, which can cooperatively regulate and control an arachidonic acid inflammation pathway, a glutathione antioxidant pathway and a tricarboxylic acid cycle energy pathway are reversely screened out. Animal experiments prove that the composition can reduce the volume of pulmonary nodules by 58.3%, human body application shows that the nodules of 76.7% of subjects are reduced, and liver and kidney toxicity is avoided. The composition can be prepared into tablets, capsules and other dosage forms, can be used as functional food or adjuvant therapy drugs for high risk groups of pulmonary nodules, and realizes accurate nutrition intervention.

The invention discloses a wild breeding optimization method for cordyceps sinensis, and relates to the technical field of cordyceps sinensis breeding. The cordyceps sinensis wild breeding optimization method comprises the following specific steps: separating high-activity strains from wild cordyceps sinensis, optimizing a synthesis path of key components by combining the screened high-activity strains with a gene editing technology, and inoculating pupae with the finally obtained bacterial liquid. And placing in a culture column to optimize the growth speed of hyphae, completing optimization of the content of active components, and simulating plateau low-temperature and low-oxygen environment in which the culture column is located. A metabolic pathway is optimized by combining high-activity strain screening and gene editing, the synthesis efficiency of key active components such as cordycepin and adenosine is remarkably improved, an ecological column with the light transmittance being larger than or equal to 85% is constructed, key ecological parameters of the high altitude of the Qinghai-Tibet Plateau are simulated and reproduced by combining ultraviolet intensity regulation and control and a low-temperature and low-oxygen environment, and the application prospect is wide. The breeding problem of loss of active ingredients in low-altitude cultivation is solved.

The invention discloses an immunomodulatory composition based on a cordycepin-selenium nano chelate and a preparation method thereof, and belongs to the technical field of biological medicines.The immunomodulatory composition is characterized in that 15-25 nm zero-valent selenium nano particles are chelated through hydroxyl of cordycepin and N7 site bidentate coordination, and a stable five-membered ring structure with the molar ratio is formed; the preparation method comprises the following steps: reducing sodium selenite in an inert atmosphere to generate selenium colloid, chelating the selenium colloid with a cordycepin ethanol solution, and carrying out ultrafiltration and freeze-drying to obtain the product. The invention aims to solve the problems of fuzzy structure, inconsistent batch, uncontrollable immunomodulatory function and the like caused by non-specific mixing in the prior art, and the composition can bidirectionally regulate and control IRF3 pathways, promote polarization of M2 type macrophages and proliferation of Treg cells, and has a clear immunomodulatory mechanism and excellent stability.

The invention discloses a cordyceps sinensis culture control method and system, and core culture targets are set in generations by identifying the subculture generations G1, G2 and G3 of cordyceps sinensis strains: the G1 generation activates a cordycepin and adenosine synthesis pathway, the G2 generation maximizes the total nucleoside content and antioxidant activity, and the G3 generation improves the biomass and delays degradation. The system monitors environmental parameters such as temperature and humidity, illumination, gas concentration and the like, and thallus physiological indexes such as biomass, metabolism precursor concentration, active substance content and the like in real time, and adopts algorithms such as fuzzy PID, NSGA-II multi-objective optimization, model prediction control and the like to dynamically adjust culture conditions such as nutrition supply, dissolved oxygen, illumination period and the like. The method provided by the invention solves the problem that traditional static regulation neglects intergenerational difference of strains, realizes subgeneration refined targeted regulation, remarkably improves the yield and quality of target products, prolongs the service cycle of the strains, and is suitable for industrial bionic cultivation of cordyceps sinensis.

The application discloses a high-quality and high-yield cultivation method of wasp pupa cordyceps, and belongs to the field of artificial cultivation technology of rare medicinal and edible fungi. The method selects frozen wasps at each day age of mature larva stage to metamorphic stage as main raw materials of culture medium, and the culture medium is comprehensive in nutrition and high in quality. The cordyceps cultivated by the method contains high medicinal and nutritional components such as cordycepin, amino acid and cordyceps polysaccharide, and the content of cordycepin is particularly outstanding. The application has high safety and is not affected by environmental factors. The application adopts a pipette injection method, and the culture medium is prepared through normal pressure sterilization or high pressure sterilization. The number of wasp pupae inoculated per hour is 20 times that of the prior art, and the bacterial contamination rate can reach 100%. The application solves the problems of labor-intensive, time-consuming and low bacterial contamination rate of the current manual injection method, shortens the time for the insect body to be fully covered with bacteria by more than one week, and increases the yield by more than 3 times. The application is convenient for large-scale production, and has novelty, creativity and wide application value.

The invention relates to the technical field of daily chemicals, in particular to a preparation method and application of a cordyceps sinensis fermentation product. The cordyceps sinensis fermentation product is obtained by fermenting cordyceps sinensis flowers serving as a solid culture medium and lactic acid bacteria and saccharomyces cerevisiae serving as strains. According to the cordyceps sinensis probiotic fermentation composition provided by the invention, a composite solid-liquid two-stage fermentation system taking the cordyceps sinensis flowers as the raw material is constructed, and the lactic acid bacteria and the saccharomycetes are cooperatively applied for metabolic conversion, so that the content and the stability of active substances such as cordycepin, cordyceps polysaccharide, adenosine, small molecule peptide and amino acid in a fermentation product are remarkably improved; meanwhile, good skin adaptability and a skin care effect are endowed.

This invention belongs to the fields of genetic engineering and microbial technology, specifically relating to a yeast quorum sensing dynamic regulation system, method, and its applications. This invention successfully constructs a functional, bacterial-derived RpaI / RpaR quorum sensing system in yeast for the first time. The constructed yeast quorum sensing dynamic regulation system possesses advantages such as low background leakage, high sensitivity, and wide dynamic range. By flexibly coupling the core system with a signal amplification module (Gal4) and a signal conversion module (CRISPRi), a bifunctional cascade platform integrating activation and inhibition is constructed, achieving multidimensional and precise dynamic regulation of metabolic pathways. Furthermore, the system has been applied to the biosynthesis of several important compounds, including cordycepin, geraniol, and 3-hydroxypropionic acid, significantly increasing yields and effectively balancing the conflict between cell growth and product synthesis, demonstrating its great potential as a universal dynamic regulation tool.

The method comprises the following steps: S1, cleaning and drying: firstly cleaning impurities on the surface of the cordyceps militaris, and then drying the cleaned cordyceps militaris; s2, sterilization treatment: sterilizing the dried cordyceps militaris, and killing bacteria on the cordyceps militaris; s3, cordycepin extraction: soaking the sterilized cordyceps militaris and the white spirit in an extraction container, and adjusting the soaking time and the alcohol concentration according to a cordycepin extraction efficiency formula. The cordyceps militaris wine has the advantage of high nutritional value, and solves the problems that effective components in cordyceps militaris are inconvenient to extract into the wine in the blending process of the existing wine, the cordyceps militaris is rich in various components beneficial to human bodies, such as cordycepin and adenosine, and if the components are not fully dissolved in the wine, the components cannot be fully dissolved in the wine. And the nutritional value of the final product is greatly reduced.

The invention belongs to the technical field of gene engineering, and provides a method for improving cordycepin synthesized by a strain through lipid droplet engineering modification and application. Based on the characteristic that key enzymes (ketodeoxyadenosine reductase and adenylate dephosphatase) synthesized by cordycepin are positioned on lipid droplets, the synthesis efficiency of the product is improved by increasing the number or volume of the lipid droplets. The lipid droplet engineering modification comprises the following steps: intensively expressing a dga1 gene, an lro1 gene, a pah1 gene or an acc1 gene; the hrd1 gene, the erde1 gene, the opi3 gene or the fld1 gene are inactivated / weakened; cu < 2 + > is exogenously added into the culture medium. Wherein the hrd1 and the erdes 1 are inactivated / weakened at the same time, so that the number of lipid droplets can be increased synergistically, and the yield of cordycepin is increased by 37.7%. The production efficiency of cordycepin is remarkably improved through targeted lipid droplet regulation and control, and the method has the potential of industrial application.

Provided is a culture medium for enhancing the content of cordycepin from Cordyceps militaris, wherein the culture medium comprises Hermetia illucens adult insects. According to the present invention, the culture medium can ensure a significantly higher cordycepin yield than conventional grain-based media or pupa-based media by means of a method for extracting cordycepin from Cordyceps militaris cultured using Hermetia illucens adult insects as a host.

The present invention relates to a method for producing a fermented powder of Cordyceps militaris with enhanced active ingredients by culturing Cordyceps militaris mycelium using sword beans and brown rice as culture media, and extracting and pulverizing the mycelium. Specifically, the method comprises the steps of: (1) preparing a sterile culture medium by mixing pre-treated sword beans and brown rice in a certain ratio; (2) inoculating the Cordyceps militaris mycelium with accession number KCCM13296P into the culture medium and performing dark culture and photoculture; (3) preparing a complex fermented product by sterilizing, drying, and grinding the culture; and (4) preparing a final powder by adding ethanol to the fermented product to extract active ingredients, and concentrating and freeze-drying the extract. The fermented powder produced in this manner has increased content of beneficial components such as β-glucan, ergosterol, cordycepin, and adenosine, and exhibits excellent inhibitory effects on NO and inflammatory cytokines, making it useful as a raw material for health functional foods or pharmaceuticals.

1. The name of the design product: Cordyceps freeze-dried goat milk powder packaging box. 2. The use of the design product: for packaging Cordyceps freeze-dried goat milk powder. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: front view.

The present disclosure is in the field of biomedicine and particularly relates to a therapeutic agent for the treatment of mitochondrial disorder and promoting mitochondrial function in a subject. The method comprises administering nucleoside derivative cordycepin CO1, its derivatives and a pharmaceutically acceptable carrier to a subject. Also provided is cordycepin, its derivatives and a pharmaceutical composition thereof.

The present invention relates to: a method for cultivating Cordyceps, comprising a step of inoculating a starter culture of Cordyceps into a mixed medium of hemp seeds and brown rice and culturing same; Cordyceps grown by the method; and a method for increasing the cordycepin and adenosine contents of Cordyceps.

The invention relates to the field of biochemistry, in particular to cordycepin and a preparation method thereof. The catalyst applied to cordycepin comprises red phosphorus or / and black scale. The preparation method comprises the following step: carrying out reduction reaction on a halogenated product of organic acylated adenosine, a catalyst and a reducing agent under light irradiation to obtain the cordycepin. The dehalogenation reaction is promoted through a photocatalysis mechanism under the irradiation of light. Under the irradiation of light, halide ions in the halogenated product are effectively removed to form cordycepin. Light irradiation can excite red phosphorus to generate free radicals, the free radicals are transmitted to a reducing agent (such as rongalite to generate sulfur dioxide free radicals) through electrons, the cordycepin can be stably prepared, the purity of the cordycepin reaches 95% or above, and the yield can reach 82%.

The invention relates to the technical field of food processing, and discloses a medicinal and edible Chinese wolfberry fruit composite primary pulp formula and a preparation method thereof, the medicinal and edible Chinese wolfberry fruit composite primary pulp formula comprises the following components by weight: 65-75% of dried Chinese wolfberry fruit, 18-22% of cordyceps militaris mycelium, and 8-12% of fresh emblic leafflower fruit; the method further comprises the following steps: by taking the cleaning and crushing of medlar and emblic leafflower fruit as a starting point, degrading cell walls by stages through gradient enzymolysis, and dynamically regulating and controlling the carbon nitrogen ratio by combining with the liquid fermentation of cordyceps militaris to activate the synthesis of cordycepin; heat-sensitive components are protected through cold plasma non-thermal sterilization, polysaccharide-protein self-assembly is induced at low temperature to form nanoparticles to construct a stable system, and finally filling and storage are performed. Activity synergistic interaction is achieved through molecular compatibility and a gradient regulation and control process, thermosensitive activity is accurately protected through cold plasma-nitrogen combined sterilization, a nano stable system is constructed through polysaccharide-protein self-assembly, and a natural anti-corrosion barrier is formed through polyphenol multi-target bacteriostasis.

The invention provides a ketodeoxyadenosine reductase mutant and application thereof in biosynthesis of cordycepin. The invention relates to a method for improving the yield of cordycepin by constructing the ketodeoxyadenosine reductase mutant. The method comprises the following steps: mutating ketodeoxyadenosine reductase, and screening to obtain a mutant containing any one or a combination of more of key amino acid site mutations such as N19S, A31T, L124P, Y256F, V387I, G406A, A485V and V666A. The invention also provides a high-throughput screening method of the mutant, an expression vector and a host cell comprising the nucleotide, and a fermentation process for producing cordycepin by using the mutant. When the ketodeoxyadenosine reductase mutant disclosed by the invention is expressed in saccharomyces cerevisiae, the yield of cordycepin is increased by 6%-36.8%.

The invention discloses a yak bone polypeptide and cordyceps militaris extract nano-composite and a method for determining the content of cordycepin, cordycepic acid and adenosine in a product of the yak bone polypeptide and cordyceps militaris extract nano-composite, and belongs to the technical field of food and drug quality detection. According to the method, dynamic ultrasonic-assisted extraction is creatively combined with a dual purification technology, and high-sensitivity detection is carried out in combination with a high performance liquid chromatography-triple quadrupole mass spectrometry method. Specifically, efficient release of target components is achieved through 40% methanol aqueous solution with the temperature controlled to be 45 DEG C under the synergistic effect of high-speed homogenization and intermittent ultrasound, and accurate quantification is conducted in an MRM mode after protein precipitation and secondary centrifugal purification are conducted through acetonitrile. According to the method, the extraction efficiency and the detection sensitivity of the trace components in the complex nano-composite are remarkably improved, and the detection limit is as low as ng / mL level. Compared with a traditional method, the method has the advantages of being easy and convenient to operate, high in accuracy, high in matrix interference resistance and the like, and a standardized solution is provided for quality control of related products.

The invention belongs to the technical field of biological medicines, and particularly relates to a pharmaceutical preparation added with pyruvate as well as a preparation method and application of the pharmaceutical preparation. The pharmaceutical preparation added with pyruvate is prepared from the following raw materials in percentage by mass: 0.2 to 0.4 percent of cordycepin derivative, 0.1 to 0.2 percent of sodium pyruvate and the balance of normal saline, the pharmaceutical preparation added with pyruvate can effectively promote bronchial relaxation; the structural formula of the cordycepin derivative is as follows: # imgabs0 #.

The invention belongs to the field of wine brewing, and particularly relates to SOD (superoxide dismutase) cordyceps sinensis health wine and a preparation method thereof. The SOD cordyceps sinensis health-preserving wine is prepared from the following raw materials in parts by weight: 70 to 80 parts of base wine, 5 to 8 parts of cordyceps sinensis extract, 0.05 to 0.1 part of SOD microcapsules, 10 to 15 parts of traditional Chinese medicine auxiliary materials and 2 to 3 parts of corrigent. Through segmented extraction, ultrasonic treatment and segmented ageing processes, the activity of cordycepin and SOD is effectively reserved, so that the health-care wine has the effects of tonifying the kidney, benefiting the lung, resisting oxidation and protecting the liver; moreover, the product tastes mellow and mild, and the drinking health risk can be remarkably reduced.