88 results about "Cordycepin" patented technology

The invention belongs to the technical field of medicine crystals, and relates to cordycepin-oxalate as well as a preparation method and application thereof. The invention relates to a preparation method of cordycepin-oxalate. The preparation method comprises the following steps: (1) physical mixing: sufficiently and uniformly mixing oxalic acid and cordycepin-crystal form II crystals; (2) liquid auxiliary grinding: adding an auxiliary agent, and continuously grinding to obtain a solid mixture; and (3) drying the solid mixture to obtain the cordycepin-oxalate. According to the method, the cordycepin-oxalic acid eutectic salt is prepared for the first time, the dissolution rate of the cordycepin-oxalic acid eutectic salt is obviously lower than the dissolution rate of cordycepin reported in the prior art, and the cordycepin-oxalic acid eutectic salt has extremely high potential medicinal application value. The cordycepin-oxalic acid eutectic salt has important technical innovation and market application potential due to the slow release effect.

The invention relates to the technical field of mycelium culture, and provides a culture method and application of cordyceps militaris mycelium with high selenium content and high cordycepin content. The culture method comprises the following steps: inoculating cordyceps militaris mycelia into a liquid culture medium, and carrying out shake culture for 4-7 days to obtain cordyceps militaris liquid seeds; adding a plant selenium source into the solid culture medium, sterilizing to obtain a cordyceps militaris selenium-rich solid culture medium, adding the cordyceps militaris liquid seeds into the cordyceps militaris selenium-rich solid culture medium, and continuously culturing for 30-40 days to obtain the high-selenium and high-cordycepin cordyceps militaris mycelium. According to the culture method, the steps are simple and convenient, the raw materials are cheap and safe, the total selenium content of the produced mycelium is 0.76-191.45 mg / kg, the organic selenium content is more than 99%, the cordycepin content is 17707.3-24769 mg / kg, the content of various effective components such as the total selenium, the organic selenium and the cordycepin is outstanding, the yield is high, standardized popularization and application of the selenium industry in multiple fields are facilitated, and the wide market prospect is achieved.

This invention discloses a protein-polysaccharide ternary nanoparticle loaded with cordycepin, its preparation method, and its applications. The invention employs a layer-by-layer self-assembly method to modify the surface of zein nanoparticles. Since zein has a positive surface charge, the first layer modification uses the anionic polysaccharide sodium alginate for electrostatic bonding, and the second layer modification uses the cationic polysaccharide chitosan, constructing positively charged ternary composite nanoparticles. These nanoparticles have small particle size, high encapsulation efficiency, high drug loading capacity, and a sustained-release effect, significantly reducing cytotoxicity and significantly improving the therapeutic effect on osteoarthritis. This invention develops a novel cordycepin formulation, overcoming the limitations of cordycepin's clinical use.

The invention discloses a cordyceps militaris strain with high yield of ergothioneine and application of the cordyceps militaris strain, and belongs to the technical field of microorganisms and fermentation engineering. On the basis of the screened cordyceps militaris strain with high-yield ergothioneine potential, the cordyceps militaris strain is subjected to ultraviolet mutagenesis treatment, and a cordyceps militaris mutant strain which can be stably inherited and has high-yield ergothioneine is obtained through screening. According to the method, the yield of ergothioneine, cordycepin and cordyceps adenosine in the cordyceps militaris strain is synergistically improved on the basis of precursor directional metabolism remodeling by optimizing and screening precursor substances. The extracting solution of the cordyceps militaris strain can improve the survival rate of lactobacillus reuteri at the high temperature of 50 DEG C and has a protection effect on cells, and the active ingredients of the cordyceps militaris strain can provide an antioxidant protection effect to improve the viability of the cells in an extreme environment.

The present application relates to the field of gene editing technology, bioengineering and application of microbial fermentation, and discloses a Pichia pastoris high-efficiency gene editing system, a construction method thereof and application in a high-yield cordycepin engineering strain.The construction of the Pichia pastoris CRISPR-Cas9 gene editing system mainly includes the following steps: the histidine HIS4 nutritional deficiency back complementation is used to realize the resistance-free assembly of KhCas9; the sgRNA-tRNA array and the IIS type restriction endonuclease combination are used to simplify the gene editing operation and realize the simultaneous and high-efficiency editing of multiple genes; and the Brex27 domain is fused with KhCas9 to strengthen the homologous recombination efficiency. By using the gene editing system, through the optimization of methanol assimilation, 3'-AMP and adenosine precursor supply, ATP / NADPH energy supply and the like, the construction of a cordycepin engineering strain without resistance gene residues is realized for the first time, the gene editing efficiency of Pichia pastoris is greatly improved, and a powerful tool is provided for the industrialized production of cordycepin.

The invention discloses paecilomyces hepialid powder and a production process thereof, and belongs to the technical field of microorganisms. The production process comprises the following steps: S01, slant strain preparation; s02, shaking the seed solution in a flask; s03, fermenting in a fermentation tank; s04, performing centrifugal separation; s05, drying is carried out; and S06, crushing and sieving to obtain the paecilomyces hepialid powder. According to the paecilomyces hepialid powder obtained by the invention, the yield is 42 to 55 g / L, and the adenosine content is 13.29 to 14.83 g / kg; the content of cordycepin is 2.14 to 3.08 g / kg. Static-high-temperature fermentation is introduced in the fermentation process, the obtained paecilomyces hepialid powder has the characteristics of high biomass, high adenosine content and high cordycepin content, the problems of scarcity of cordyceps sinensis resources and high medicine price can be solved, the contradiction between supply and demand can be relieved, the environment can be protected, the cost of the cordyceps sinensis preparation can be reduced, and the application prospect is wide. And the effect of a medication group of the cordyceps sinensis preparation as a tonic drug is increased.

The invention provides a pulmonary nodule targeting food and medicine homologous composition based on metabonomics screening and application thereof. By analyzing a pulmonary nodule specific metabolic profile, active components, namely platycodin D, mogroside V, oyster active peptide and fermented cordycepin, which can cooperatively regulate and control an arachidonic acid inflammation pathway, a glutathione antioxidant pathway and a tricarboxylic acid cycle energy pathway are reversely screened out. Animal experiments prove that the composition can reduce the volume of pulmonary nodules by 58.3%, human body application shows that the nodules of 76.7% of subjects are reduced, and liver and kidney toxicity is avoided. The composition can be prepared into tablets, capsules and other dosage forms, can be used as functional food or adjuvant therapy drugs for high risk groups of pulmonary nodules, and realizes accurate nutrition intervention.

The invention discloses a wild breeding optimization method for cordyceps sinensis, and relates to the technical field of cordyceps sinensis breeding. The cordyceps sinensis wild breeding optimization method comprises the following specific steps: separating high-activity strains from wild cordyceps sinensis, optimizing a synthesis path of key components by combining the screened high-activity strains with a gene editing technology, and inoculating pupae with the finally obtained bacterial liquid. And placing in a culture column to optimize the growth speed of hyphae, completing optimization of the content of active components, and simulating plateau low-temperature and low-oxygen environment in which the culture column is located. A metabolic pathway is optimized by combining high-activity strain screening and gene editing, the synthesis efficiency of key active components such as cordycepin and adenosine is remarkably improved, an ecological column with the light transmittance being larger than or equal to 85% is constructed, key ecological parameters of the high altitude of the Qinghai-Tibet Plateau are simulated and reproduced by combining ultraviolet intensity regulation and control and a low-temperature and low-oxygen environment, and the application prospect is wide. The breeding problem of loss of active ingredients in low-altitude cultivation is solved.

The invention discloses an immunomodulatory composition based on a cordycepin-selenium nano chelate and a preparation method thereof, and belongs to the technical field of biological medicines.The immunomodulatory composition is characterized in that 15-25 nm zero-valent selenium nano particles are chelated through hydroxyl of cordycepin and N7 site bidentate coordination, and a stable five-membered ring structure with the molar ratio is formed; the preparation method comprises the following steps: reducing sodium selenite in an inert atmosphere to generate selenium colloid, chelating the selenium colloid with a cordycepin ethanol solution, and carrying out ultrafiltration and freeze-drying to obtain the product. The invention aims to solve the problems of fuzzy structure, inconsistent batch, uncontrollable immunomodulatory function and the like caused by non-specific mixing in the prior art, and the composition can bidirectionally regulate and control IRF3 pathways, promote polarization of M2 type macrophages and proliferation of Treg cells, and has a clear immunomodulatory mechanism and excellent stability.

The invention discloses a cordyceps sinensis culture control method and system, and core culture targets are set in generations by identifying the subculture generations G1, G2 and G3 of cordyceps sinensis strains: the G1 generation activates a cordycepin and adenosine synthesis pathway, the G2 generation maximizes the total nucleoside content and antioxidant activity, and the G3 generation improves the biomass and delays degradation. The system monitors environmental parameters such as temperature and humidity, illumination, gas concentration and the like, and thallus physiological indexes such as biomass, metabolism precursor concentration, active substance content and the like in real time, and adopts algorithms such as fuzzy PID, NSGA-II multi-objective optimization, model prediction control and the like to dynamically adjust culture conditions such as nutrition supply, dissolved oxygen, illumination period and the like. The method provided by the invention solves the problem that traditional static regulation neglects intergenerational difference of strains, realizes subgeneration refined targeted regulation, remarkably improves the yield and quality of target products, prolongs the service cycle of the strains, and is suitable for industrial bionic cultivation of cordyceps sinensis.

The application discloses a high-quality and high-yield cultivation method of wasp pupa cordyceps, and belongs to the field of artificial cultivation technology of rare medicinal and edible fungi. The method selects frozen wasps at each day age of mature larva stage to metamorphic stage as main raw materials of culture medium, and the culture medium is comprehensive in nutrition and high in quality. The cordyceps cultivated by the method contains high medicinal and nutritional components such as cordycepin, amino acid and cordyceps polysaccharide, and the content of cordycepin is particularly outstanding. The application has high safety and is not affected by environmental factors. The application adopts a pipette injection method, and the culture medium is prepared through normal pressure sterilization or high pressure sterilization. The number of wasp pupae inoculated per hour is 20 times that of the prior art, and the bacterial contamination rate can reach 100%. The application solves the problems of labor-intensive, time-consuming and low bacterial contamination rate of the current manual injection method, shortens the time for the insect body to be fully covered with bacteria by more than one week, and increases the yield by more than 3 times. The application is convenient for large-scale production, and has novelty, creativity and wide application value.

This invention belongs to the fields of genetic engineering and microbial technology, specifically relating to a yeast quorum sensing dynamic regulation system, method, and its applications. This invention successfully constructs a functional, bacterial-derived RpaI / RpaR quorum sensing system in yeast for the first time. The constructed yeast quorum sensing dynamic regulation system possesses advantages such as low background leakage, high sensitivity, and wide dynamic range. By flexibly coupling the core system with a signal amplification module (Gal4) and a signal conversion module (CRISPRi), a bifunctional cascade platform integrating activation and inhibition is constructed, achieving multidimensional and precise dynamic regulation of metabolic pathways. Furthermore, the system has been applied to the biosynthesis of several important compounds, including cordycepin, geraniol, and 3-hydroxypropionic acid, significantly increasing yields and effectively balancing the conflict between cell growth and product synthesis, demonstrating its great potential as a universal dynamic regulation tool.

The method comprises the following steps: S1, cleaning and drying: firstly cleaning impurities on the surface of the cordyceps militaris, and then drying the cleaned cordyceps militaris; s2, sterilization treatment: sterilizing the dried cordyceps militaris, and killing bacteria on the cordyceps militaris; s3, cordycepin extraction: soaking the sterilized cordyceps militaris and the white spirit in an extraction container, and adjusting the soaking time and the alcohol concentration according to a cordycepin extraction efficiency formula. The cordyceps militaris wine has the advantage of high nutritional value, and solves the problems that effective components in cordyceps militaris are inconvenient to extract into the wine in the blending process of the existing wine, the cordyceps militaris is rich in various components beneficial to human bodies, such as cordycepin and adenosine, and if the components are not fully dissolved in the wine, the components cannot be fully dissolved in the wine. And the nutritional value of the final product is greatly reduced.

Provided is a culture medium for enhancing the content of cordycepin from Cordyceps militaris, wherein the culture medium comprises Hermetia illucens adult insects. According to the present invention, the culture medium can ensure a significantly higher cordycepin yield than conventional grain-based media or pupa-based media by means of a method for extracting cordycepin from Cordyceps militaris cultured using Hermetia illucens adult insects as a host.

The present invention relates to a method for producing a fermented powder of Cordyceps militaris with enhanced active ingredients by culturing Cordyceps militaris mycelium using sword beans and brown rice as culture media, and extracting and pulverizing the mycelium. Specifically, the method comprises the steps of: (1) preparing a sterile culture medium by mixing pre-treated sword beans and brown rice in a certain ratio; (2) inoculating the Cordyceps militaris mycelium with accession number KCCM13296P into the culture medium and performing dark culture and photoculture; (3) preparing a complex fermented product by sterilizing, drying, and grinding the culture; and (4) preparing a final powder by adding ethanol to the fermented product to extract active ingredients, and concentrating and freeze-drying the extract. The fermented powder produced in this manner has increased content of beneficial components such as β-glucan, ergosterol, cordycepin, and adenosine, and exhibits excellent inhibitory effects on NO and inflammatory cytokines, making it useful as a raw material for health functional foods or pharmaceuticals.

1. The name of the design product: Cordyceps freeze-dried goat milk powder packaging box. 2. The use of the design product: for packaging Cordyceps freeze-dried goat milk powder. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: front view.

The present disclosure is in the field of biomedicine and particularly relates to a therapeutic agent for the treatment of mitochondrial disorder and promoting mitochondrial function in a subject. The method comprises administering nucleoside derivative cordycepin CO1, its derivatives and a pharmaceutically acceptable carrier to a subject. Also provided is cordycepin, its derivatives and a pharmaceutical composition thereof.

The invention provides a ketodeoxyadenosine reductase mutant and application thereof in biosynthesis of cordycepin. The invention relates to a method for improving the yield of cordycepin by constructing the ketodeoxyadenosine reductase mutant. The method comprises the following steps: mutating ketodeoxyadenosine reductase, and screening to obtain a mutant containing any one or a combination of more of key amino acid site mutations such as N19S, A31T, L124P, Y256F, V387I, G406A, A485V and V666A. The invention also provides a high-throughput screening method of the mutant, an expression vector and a host cell comprising the nucleotide, and a fermentation process for producing cordycepin by using the mutant. When the ketodeoxyadenosine reductase mutant disclosed by the invention is expressed in saccharomyces cerevisiae, the yield of cordycepin is increased by 6%-36.8%.

The invention discloses a yak bone polypeptide and cordyceps militaris extract nano-composite and a method for determining the content of cordycepin, cordycepic acid and adenosine in a product of the yak bone polypeptide and cordyceps militaris extract nano-composite, and belongs to the technical field of food and drug quality detection. According to the method, dynamic ultrasonic-assisted extraction is creatively combined with a dual purification technology, and high-sensitivity detection is carried out in combination with a high performance liquid chromatography-triple quadrupole mass spectrometry method. Specifically, efficient release of target components is achieved through 40% methanol aqueous solution with the temperature controlled to be 45 DEG C under the synergistic effect of high-speed homogenization and intermittent ultrasound, and accurate quantification is conducted in an MRM mode after protein precipitation and secondary centrifugal purification are conducted through acetonitrile. According to the method, the extraction efficiency and the detection sensitivity of the trace components in the complex nano-composite are remarkably improved, and the detection limit is as low as ng / mL level. Compared with a traditional method, the method has the advantages of being easy and convenient to operate, high in accuracy, high in matrix interference resistance and the like, and a standardized solution is provided for quality control of related products.

The invention belongs to the field of wine brewing, and particularly relates to SOD (superoxide dismutase) cordyceps sinensis health wine and a preparation method thereof. The SOD cordyceps sinensis health-preserving wine is prepared from the following raw materials in parts by weight: 70 to 80 parts of base wine, 5 to 8 parts of cordyceps sinensis extract, 0.05 to 0.1 part of SOD microcapsules, 10 to 15 parts of traditional Chinese medicine auxiliary materials and 2 to 3 parts of corrigent. Through segmented extraction, ultrasonic treatment and segmented ageing processes, the activity of cordycepin and SOD is effectively reserved, so that the health-care wine has the effects of tonifying the kidney, benefiting the lung, resisting oxidation and protecting the liver; moreover, the product tastes mellow and mild, and the drinking health risk can be remarkably reduced.

The application discloses a 3'-deoxyadenosine compound and application thereof in preparation of an antitumor drug. The 3'-deoxyadenosine compound and the pharmaceutical composition thereof have good antitumor proliferation effect. Compared with a parent drug, the 3'-deoxyadenosine compound has better affinity to a cell membrane, so that the half-life of in-vivo metabolism of the drug is longer, and the drug stays in the body for a longer time. Compared with other nucleoside antitumor drugs, the cordycepin derivative and the pharmaceutical composition thereof have a wider range of tumor types and effects, including excellent inhibition effect on gastric cancer, pancreatic cancer, liver cancer, small cell lung cancer, colorectal cancer, melanoma, ovarian cancer and the like, and the side effect is lower and the curative effect is better.

The present application provides a kind of Cordyceps militaris pre-made noodles and its preparation method, belong to food processing technical field especially.The present application is prepared by compounding wheat flour, Cordyceps militaris fruiting body ultrafine powder, Cordyceps militaris fermented wheat flour and Cordyceps militaris fruiting body enzymatic solution, supplemented with konjac flour, gluten powder, a kind of pre-made noodles with Cordyceps militaris flavor and nutritional activity rich in cordycepin, cordyceps polysaccharide, cordyceps acid, adenosine, flavone and vitamin.The pre-made noodles of the present application are smooth in taste, rich in functional components, enrich the market product variety of Cordyceps militaris, solve the existing Cordyceps militaris noodle poor toughness, easy to break during cooking process, easy to paste soup, taste and taste is not good, Cordyceps militaris addition is less, actual nutritional effect is insufficient, and cooking process nutrition is easy to lose, etc.Problems, realize the high value utilization of Cordyceps militaris, promote the application range of Cordyceps militaris, bring the Cordyceps militaris delicious and nutritious to more consumers.

The application discloses a kind of scalable production's bee pupa cordyceps cultivation method, belong to precious edible mushroom artificial cultivation technical field, select frozen honeybee in mature larva period to metamorphosis period each day old pupa body as culture medium main raw material, through normal pressure sterilization or high pressure sterilization production culture medium, using pipette gun injection method, such as every hour inoculation amount is 20 times of prior art, bee pupa cordyceps culture medium dyeing bacteria success rate can reach more than 98%, such as solve the current by hand injection method inoculation speed slow, time-consuming, dyeing bacteria rate poor drawbacks, and dyeing bacteria period time can be shortened by more than one week, bee pupa cordyceps single bottle yield is more than 2 times of prior art, high safety performance, it is favorable to scale production.The bee pupa cordyceps active ingredient cordycepin content is very high, medicinal value is big, development prospect is wide, not only can drive more beekeeping person to make money, also opens another piece of sky of precious medicinal and edible mushroom industrial production.

The invention discloses a pharmaceutical composition for adjuvant therapy of nodular diseases and a preparation method thereof, and relates to the technical field of medicines.The pharmaceutical composition is prepared from, by weight, 1-10 parts of cordycepin, 10-20 parts of spica prunellae extract, 10-30 parts of plant extract for softening hardness to dissipate stagnation and 15-30 parts of functional auxiliary materials; the cordyceps nucleoside is a nucleoside component of which the purity is greater than or equal to 98%; the mass ratio of oleanolic acid to ursolic acid in the prunella vulgaris extract is 3: 1, and the total content of oleanolic acid and ursolic acid is greater than or equal to 12%; the hard lump resolving plant extract is an extract of thunberg fritillary bulb, radix scrophulariae, oyster powder, seaweed, kelp, radix curcumae and radix bupleuri; the functional auxiliary material is formed by compounding at least three of a filling agent, a disintegrating agent, a suspending aid and an adhesive according to an equal mass ratio; the multiple raw materials are scientifically proportioned to play a synergistic role, the preparation process is standardized, active ingredients are reserved, indexes of finished products are stable, and large-scale production and adjuvant therapy of the nodular diseases can be achieved.

The application discloses a method for preparing functional caragana fiber compound feed and belongs to the technical field of feed processing. The method comprises the following steps: taking caragana as raw material, performing solid-liquid separation after dilute sulfuric acid pretreatment, preparing cordycepin and lysine through liquid state fermentation of cordyceps militaris and glutamic acid corynebacterium respectively, neutralizing caragana solid fiber and caragana fiber raw material obtained through ammonia treatment, and preparing fiber feed through solid anaerobic fermentation of the compound fermentation strain, and finally, compounding the three to obtain functional caragana fiber compound feed. The method realizes efficient utilization of all components of caragana sugar and fiber resources, and the prepared compound feed is rich in cordycepin, lysine and dietary fiber, the crude protein content of the fiber feed is more than 20%, and the fiber feed is rich in cordycepin and lysine functional components. The method not only provides a new way for resource utilization and high value utilization of caragana, but also provides a new idea for research and development of functional coarse feed, and has good economic, ecological and industrial popularization value.

The application discloses a Leuconostoc strain and application thereof, and the Leuconostoc strain HX-236 is screened by the inventors. The Leuconostoc strain HX-236 uses cordyceps militaris powder as the only nitrogen source, and after fermentation culture, a fermentation filtrate can be obtained. The fermentation filtrate has high contents of cordycepin and cordycepic acid, is easily soluble in water, is convenient to use, has good stability, has good compatibility, can significantly improve the expression of hypoxia inducible factor (HIF-1 alpha) in cells, thereby activating downstream signal pathways for regulating inflammatory reactions, cell autophagy and tissue neogenesis, and then regulating cell hypoxia pressure, improving problems of skin aging such as cell activity reduction, collagen loss, pigmentation and skin elasticity reduction caused by hypoxia, and having wide application prospects in the fields of medicines, cosmetics and the like.

The invention relates to the technical field of strain breeding and fermentation, in particular to a cordyceps militaris strain and application thereof. According to the invention, an original strain EF-34 is subjected to sexual propagation and ARTP mutation breeding screening to obtain a cordyceps militaris strain with high yield of cordycepin, stable fermentation and no degradation, the cordyceps militaris strain is classified and named as Cordyceps militaris, the strain number is EF-34-Z-1, the cordyceps militaris strain is preserved in Guangdong Microbial Culture Collection Center on March 17, 2025, the preservation number is GDMCC No: 66025, and the preservation number is GDMCC No: 66025. The preservation address is the fifth floor of the building 59, No.100 courtyard, Xianlie Middle Road, Guangzhou City. The strain not only contains a whole set of genomes of cordyceps militaris, but also contains characteristic fragments MAT1-1 and MAT1-2. In the ton-scale production, fermentation and culture process of cordycepin, the strain can be repeatedly and stably operated for multiple batches, the yield is increased accumulatively, and the strain has a wide application prospect.

The application discloses a compound with anticancer effect based on 3'-deoxyadenosine modified by chemical modification, and a structure of the compound is shown as formula I. The cordycepin derivative and the pharmaceutical composition thereof have good antitumor proliferation effect. Compared with the parent drug, the cordycepin derivative has better affinity to the cell membrane, so that the half-life of the drug metabolism in the body is longer, and the drug stays in the body for a longer time. Compared with other nucleoside antitumor drugs, the cordycepin derivative and the pharmaceutical composition thereof have a wider range of tumor types and effects, including excellent inhibition effect on gastric cancer, pancreatic cancer, liver cancer, small cell lung cancer, colorectal cancer, melanoma, ovarian cancer and the like, and the side effect is lower and the curative effect is better.

The application discloses a kind of production cordycepin yeast engineering bacteria and application.The yeast engineering bacteria is inserted in the genome of yeast cordycepin biosynthesis module, and purine metabolic pathway overexpression module or ATP and precursor supply module;The cordycepin biosynthesis module is composed of Cns1 gene and Cns2 gene;The purine metabolic pathway overexpression module is composed of ADE4 gene and Cns3-NK gene;The ATP and precursor supply module is composed of PGK gene and Cns3-NK gene.The application is according to the biosynthesis pathway of cordycepin and its metabolic regulation, constructs and optimizes the biosynthesis integrated module required in yeast in vivo cordycepin, creates yeast cell factory, and establishes microbial fermentation production cordycepin technical system with cheap carbon source sugarcane molasses as raw material, realizes the efficient, green, safe production of cordycepin.

The invention discloses a preparation method of a cordyceps militaris fermentation filtrate, the cordyceps militaris fermentation filtrate and application, the preparation method comprises the following steps: mixing a cordyceps militaris strain with a liquid culture medium, dispersing and culturing to obtain a high-density cordyceps militaris strain seed solution; inoculating the high-density cordyceps militaris seed solution into a fermentation culture medium for fermentation, adding Tween 80 in the middle and later periods of the logarithmic growth phase of microorganisms in the fermentation process, adding sophorolipid in the stable growth phase of the microorganisms, and obtaining a mixed fermentation solution after the fermentation is finished; and carrying out inactivation, centrifugation, freeze thawing, filtration, re-inactivation and preservative treatment on the mixed fermentation liquid to obtain the cordyceps militaris fermentation filtrate. The cordyceps militaris fermentation filtrate prepared by the method disclosed by the invention is high in cordycepin content, strong in oxidation resistance and relatively high in stability, and has a very good application prospect in cosmetics, health care products or raw material medicines.