4066 results about "Saccharomyces cerevisiae" patented technology

The present invention provides a highly sensitive method of diagnosing inflammatory bowel disease (IBD) in an individual. The method includes the steps of isolating a sample from the individual; determining by non-histological means whether the sample is positive for anti-neutrophil cytoplasmic antibodies (ANCA); determining whether the sample is positive for anti-Saccharomyces cerevisiae immunoglobulin A (ASCA-IgA); determining whether the sample is positive for anti-Saccharomyces cerevisiae immunoglobulin G (ASCA-IgG); and diagnosing the individual as having IBD when the sample is positive for ANCA, ASCA-IgA or ASCA-IgG, and diagnosing the individual as not having IBD when the sample is negative for ANCA, ASCA-IgA and ASCA-IgG, provided that the method does not include histological analysis of neutrophils.

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention relates to a fermentation method for production of nutritionally balanced natural fruit enzymes. The method comprises the following steps: (1) pretreatment on raw materials; (2) a first bacterium inoculation: inoculating a saccharomyces cerevisiae liquor with concentration of 106 CFU / ml into a pulp according to an inoculation proportion of 5%; (3) early stage fermentation; (4) a secondary bacterium inoculation: after the early fermentation, inoculating acetic acid bacterium liquid and lactic acid bacterium liquid in a weight ratio of 1:1 for a secondary bacterium inoculation according to an inoculation amount of 5%; (5) middle-term fermentation; (6) coarse filtration; (7) secondary fermentation; (8) ripening; and (9) fine filtration to obtain the natural fruit enzyme. The fermentation method provided by the invention through low-temperature sterilization and strict control on the fermentation temperature retains the enzyme activity to the maximum; and twice inoculation of dominant bacteria effectively inhibits breeding of other infectious microbes, so as to realize stable product quality.

The present invention relates to microbial fodder additive, and a kind of microbial fodder additive comprising beneficial microbes and their metabolites and its preparation process and use. The microbial fodder additive is the mixture of fermented liquid or dry powder obtained with Bacillus subtilis, Bacillus cereus, lactobacillus and Saccharomyices cerevisiae and through high density deep layer liquid fermentation. It may be prepared into different specific liquid or powder preparations. The microbial fodder additive has high live bacillus content, long product preserving period and less foreign bacteria contamination, and has the functions of raise fodder utilization, promote animal's growth, increase animal product yield and quality, etc.

The invention discloses an organic matter decomposing inoculant, and a preparation method and application thereof. The organic matter decomposing inoculant comprises the following ingredients in part by weight: 10 to 20 parts of aspergillus niger microbial inoculum, 10 to 20 parts of lactobacillus plantarum microbial inoculum, 20 to 30 parts of saccharomyces cerevisiae microbial inoculum and 40 to 60 parts of bacillus subtilis microbial inoculum. The organic matter decomposing inoculant mainly comprises various microbial floras with mutual promotion and mutual synergy effects. The organic matter decomposing inoculant can decompose various organic matters quickly, can effectively kill germs, ova and weed seeds in the decomposed raw materials, can reduce diseases, weed and insect pests, andcan remove water and odor. The organic matter decomposing inoculant can stimulate growth and development of crops, can improve disease resistance, drought resistance and cold resistance, can increasesoil nutrients, can improve the soil structure, and can increase the utilization ratio of chemical fertilizer. The organic matter decomposing inoculant can be used directly and can also be used for producing commercial organic fertilizer, biological organic fertilizer, organic and inorganic complex fertilizer, biological organic and inorganic complex fertilizer and the like.

The invention relates to a novel molecular cloning method, and in particular to a method for obtaining recombinant vectors by modifying initial vectors through combined utilization of a CRISPR/Cas9 system and a saccharomyces cerevisiae cell endogenous gene homologous recombination system; only through one-time transformation and selection operations, the method can simultaneously complete insertion of ore or more target DNA fragments into the initial vectors, deletion of one or more DNA fragments from the initial vectors and/or substitution of one or more DNA fragments on the vectors for one or more target DNA fragments. The invention further relates to a reagent kit for conveniently and effectively applying the method provided by the invention.

The invention discloses a micro-ecological fruit-vegetable enzyme preparation method. The method comprises the steps of culturing 4 to 6 of six probiotics including lactobacillus acidophilus, bacillus coagulans, bacillus natto, saccharomyces cerevisiae, enterococcus faecium and bifidobacterium in a culture medium comprising ingredients such as brown sugar, liquorice and fresh sweet potato, and then culturing the probiotics through a diluted culture liquid containing brown sugar and liquorice in an activating manner to prepare a micro-ecological preparation, wherein the prepared micro-ecological preparation is a fermenting agent which is used for fermenting fruit pulp of fresh fruit and vegetable and traditional Chinese herbs. The microorganism enzyme in the ecological preparation and the natural vegetable enzyme in fruits, vegetables and Chinese herbs are compounded into a composite enzyme supplement by utilizing a micro-ecological fermentation technology, i.e. the micro-ecological fruit-vegetable enzyme. The micro-ecological fruit-vegetable enzyme can be made into a liquid type or a solid type (powder). An ideal effect is achieved in the fields such as medicine, healthcare and beauty.

Recombinant expression of fat-1 gene of Caenorhabditis elegans in a wide variety of cells, including cells of Arabidopsis thaliana and Saccharomyces cerevisiae, produces a polypeptide having ω-3 desaturase activity.

A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg/g, the reducing sugar reaches 20-80mg/g, the GABA reaches 0.317-0.5mg/g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu/g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU/g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

The invention provides compound probiotics fermented Chinese herbal medicine active health care liquid and a preparation method thereof. The health care liquid is prepared by the following raw materials: folium ginkgo, gingko pollen, fructus lycii, tea leaves, bacillus natto, saccharomyces cerevisiae, lactobacilli, acetobacter xylinum, bifidobactirium, white granulated sugar, brown sugar, defatted soy flour, honey, oligosaccharide, sodium chloride, deionized water and the like. The compound probiotics fermented Chinese herbal medicine active health care liquid integrates disease treatment and health care functions of several single products, contains Chinese herbal medicine active ingredients with the functions of preventing and treating hyperlipidemia, hyperglycemia and hypertension, preventing tumors and cancers, enhancing memory, protecting livers, improving immunity, preventing and treating coronary heart diseases, and also contains probiotics groups and metabolic products of the probiotics groups which can regulate balance in the stomach and intestines, resist aging, prolong the life, facilitate digestion, expel toxin for beauty, and prevent and treat thrombi, constipation and diarrhea. The health care liquid integrates health care and treatment, is very wide in market prospect, and conforms to the existing medical viewpoint of people that the prevention, health care, treatment and recovery are combined.

The invention provides a feed fermenting agent, fermented feed and a preparation method thereof, and relates to the technical field of animal product cultivation. The feed fermenting agent is mainly prepared from lactobacillus plantarum, enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, lactobacillus cellobiosas, a complex enzyme preparation and the like. The fermented feed is prepared from the feed fermenting agent and fermentation raw materials by using a preparation method of carrying out fermentation in a device which is in one-way exhaust. According to the feed fermenting agent and the fermented feed, disclosed by the invention, the problems of high livestock cultivation cost caused by poor nutritive value, low animal digestion and absorption rate and abuse of antibiotic drugs during cultivation, long fermentation time and slow fermentation starting of existing livestock breeding feed are solved, and high absorption and conversion rate of nutrient contents of the fermented feed are achieved; the fermented feed contains a large amount of probiotics for protecting intestine health of animals, so that the organism immunity of the animals is increased, and the use of antibiotics during livestock cultivation is reduced.

The invention discloses a biological method for quickly preparing fermented pickle, belongs to the field of vegetable deep processing and in particular relates to a method for producing the pickle by using composite bacteria powder. The invention solves the technical problem to quickly produce the high-quality fermented pickle. The method comprises the following main steps of: (1) adding materials and sealing: cutting vegetable raw materials, putting into a container, adding the composite bacteria powder containing acetobacter aceti and saccharomyces cerevisiae, and auxiliary materials containing table salt, and sealing the container; (2) fermenting: performing early fermentation at the temperature of between 23 and 26DEG C, performing after fermentation at the temperature of between 15 and 25DEG C, and controlling the total fermentation time to be 20-24hours; and (3) dehydrating and blending: removing water from the pickle after the fermentation is finished, adding seasonings, and uniformly mixing. The method is suitable for pickle production in different scales, the production time can be obviously shortened, and the pickle product is rich in nutrients and pure in taste; and the product has a wide application prospect in the fixed of pickle production.

The invention provides a preparation method for feed without antibiotics. Feed without antibiotics is complete pig feed without antibiotics, which is prepared from alkali protease and fermenting mixing feed of wholesome funguses and contains the wholesome funguses, a complex enzyme, complex organic acid and other microbe metabolites. The preparation method comprises the preparation steps: weighingthe raw materials of the fermenting feed: corn flour, bean dregs, fermenting bean dregs, fish meal and whey mist which are uniformly mixed; weighing immune polysaccharide, edible salt, calcium hydrophosphate, calcium carbonate, lysine, methionine, various vitamins and various minerals which are uniformly mixed; preparing wine making yeast, alkyl producing pseudofilament yeast, lactobacillus acidophilus, bacillus subtilis and bacillus licheniformis which are uniformly mixed; uniformly mixing all the raw materials and the funguses; adding water to the feed; bagging; sealing; fermenting until nogas is generated; discharging air; sealing again; sealing an outer packing bag and labeling to obtain a finished product. The feed without antibiotics prepared by the method has the advantages of good mouth feel, high nutritious value, easy assimilation and wide market prospect.

A low-temp nitrating bacteria preparation for removing NH3-N and COD from the seawater used in marine farm is prepared from Nitrobacter hamburgensis (AS 1.2729), Nitrobacter winogradskyi (AS 1.2730), Nitrobacter vulgaris (AS 1.2775), Bacillus cereus (AS 1.230), Bacillus licheniformis (AS 1.518) and saccharomyces cerevisiae (AS 2.412) through culturing, and proportional mixing.

The invention discloses microbial straw decomposition additive, which belongs to the field of straw decomposition additive and is obtained through combining four bacteria: bacillus subtilis, aspergillus niger, trichoderma reesei and saccharomyces cerevisiae. The microbial straw decomposition additive utilizes the temperature rise benefit of the aspergillus niger spore enzyme activity for raising the environment temperature of decomposition, the further temperature rise benefit in the growth process of the bacillus subtilis is cooperated, and the temperature required by the decomposition is ensured, so the decomposition effect of straws is improved. The microbial straw decomposition additive has the obvious temperature rise benefit and can be used for the in-site field returning decomposition of the straws, the straw decomposition can be better accelerated in low-temperature and normal-temperature environment, the bottleneck problem of straw utilization is solved, the straw burning can be effectively reduced, the decomposition work procedure and the labor cost are saved, the fertilizer feeding is reduced, the soil structure is improved, and the planting benefit is improved.

The invention relates to the field of microbial fermentation, in particular to a preparation method of a compound Daqu, so as to solve the problems of the traditional Daqu such as single nutritional components of raw materials, longer starter-making and brewing time, low utilization rate of grains, high cost and the like. The preparation method comprises the following steps: (1) treating the raw materials of the Daqu; (2) inoculating aspergillus niger 41254, aspergillus niger UV-11, monascus, mucor racemosus, actinomucor elegans, rhizopus oryzae, saccharomyces cerevisiae, candida drusei, acetobacter, bacillus subtilis and lactobacillus delbrueckii 6100 into a conical flask containing the raw materials of the Daqu for culturing, then expanding the culture and obtaining a mother culture; and(3) adopting uncooked materials for making a starter, inoculating the obtained mother culture and the raw materials of the Daqu into a solid fermentation device, keeping the temperature at 30 DEG C-33 DEG C, continuing for 30-33h, discharging the starter and finally carrying out low-temperature vacuum drying. The preparation method enriches the nutritional components in the raw materials and improves the quality of a brewed product; and a plurality of bacterial strain are artificially added, thereby improving the utilization rate of the grains, shortening the starter-making cycle, improving the yield of the brewed product and reducing the production cost.

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

The invention relates to a CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9-system-based Saccharomyces cerevisiae genome concurrent multiplex edition vector and application thereof. Cas9 protein expression cassettes and a plurality of sgRNA (small guide ribonucleic acid) scaffold expression cassettes are integrated into the vector to obtain a vector-Cas9-sgRNA scaffold, and a plurality of sgRNA segments designed and synthesized by using ADE1 as a target are integrated into the vector-Cas9-sgRNA scaffold, thereby obtaining the Saccharomyces cerevisiae genome concurrent multiplex edition vector. By adopting a simple substance granule single-copy vector system, one plasmid is utilized to simultaneously express the Cas9 protein and three sgRNA scaffolds, so that three target genes can be edited simultaneously; and thus, the multigene edition operation is very simple. Besides, the target gene edition system only needs one-step component and conversion, and thus, is simple to operate.

Disclosed is a process for extracting xylose and xylitol from a xylose mother liquor or a xylose digest, which comprises, using xylose hydrolysate or xylose mother liquid as the raw material, removing glucose through saccharomyces cerevisiae fermentation, then imitating moving bed, using water as eluent, completely separating xylose from foreign matter such as arabinose, thus obtaining component containing rich xylose.

The invention discloses a recombinant microorganism for preparing dharma diene and protopanoxadiol and a construction method of the recombinant microorganism. The construction method of the recombinant bacteria comprises a step of adding dharma diene synthase, protopanoxadiol synthase and nicotinamide adenine dinucleotide phosphate - cytochrome P450 reductase encoding gene into saccharomyces cerevisiae to obtain recombinant bacteria I. According to the recombinant microorganism for preparing the dharma diene and the protopanoxadiol and the construction method of the recombinant microorganism, by means of homologous recombination, the dharma diene synthase, the protopanoxadiol synthase and the nicotinamide adenine dinucleotide phosphate - the cytochrome P450 reductase encoding gene are all added into the saccharomyces cerevisiae to obtain the initial recombinant bacteria, and the effect that the initial recombinant bacteria can produce trace amount of the dharma diene and trace amount of the protopanoxadiol is discovered; tHMG1 activity of the initial recombinant bacteria is further improved, and therefore intermediate recombinant bacteria are obtained, and by means of the intermediate recombinant bacteria, the yield of the dharma diene and the yield of the protopanoxadiol are significantly increased; the activity of one or two or three of ERG1, ERG9 and ERG20 are improved on the basis of the intermediate recombinant bacteria, and the effect that the recombinant bacteria which can be used to increase the yield of the dharma diene and the yield of the protopanoxadiol are constructed is also discovered. By means of the recombinant microorganism for preparing the dharma diene and the protopanoxadiol and the construction method of the recombinant microorganism, the foundation is laid for artificially synthesizing the dammar diene and the protopanoxadiol.

The invention relates to a high-efficiency ferment for fermenting bean pulp, belonging to the biologic enzymolysis fermentation technical field. The high-efficiency ferment is formed by mixing bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, lactobacillus casei, saccharomyces cerevisiae and candida utilis. The fermentation technology comprises the following steps: crushing the bean pulp and corn; activating the strains; uniformly mixing the bean pulp and the fermentation liquor using a mixer; packing the mixed bean pulp in fermentation bag; fermenting the packaged bean pulp and processing fermented bean pulp: drying-crushing-detecting-packaging-finished product. The technology is simple and the product quality is stable and the content of the anti-nutrient factor in the bean pulp is eliminated and obviously reduced, therefore the bean pulp is a safe plant protein feeder for infant animal with balanced nutrient, good quality and low price and help for health growth of the infant animal, in replace of animal protein feeder of bone powder, fish powder or the like.

The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.