1494 results about "Saccharomyces cerevisiae" patented technology

The invention relates to ice wine yeast with high fermentation activity and outstanding aroma-producing characteristics and application thereof. The ice wine yeast is yeast (Saccharomyces cerevisiae) YSL16010, and the bacterial strain has been preserved in China General Microbiological Culture Collection Center on April 18, 2016, with a preservation number of CGMCC No.12349. The bacterial strain YSL16010 is obtained by separating and screening Vidal grapes from a Huanren producing area of Liaoning at a temperature of 16 DEG C in a natural fermentation process, and the ice wine wine-making yeast having good making characteristics and highly producing esters is obtained through a series of screening and identifications and small pilot scale fermentation. The ice wine wine-making yeast YSL1610 highly producing esters is used for fermenting and producing ice wine, so that the yield of volatile acid is effectively controlled while the standards of the grape wine producing countries can be met, and therefore, the content of aromatic compounds such as esters is remarkably increased. The bacterial strain can be applied to ice wine brewing industry, and can play an important role in improving the quality of the ice wine and bringing advantages of the grape producing areas into play.

The invention discloses a compound microbial agent for enhancing production of beta-phenethyl alcohol in a white spirit brewing process as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of microorganisms. The compound microbial agent is prepared from aspergillus niger, pichia membranaefaciens, Kazakstan monospora, saccharomyces cerevisiae and sporospora delbrueckii; in the compound microbial agent, the final concentration OD600 of the aspergillus niger, the final concentration OD600 of the pichia membranaefaciens, the final concentration OD600 of the Kazakstein monospora, the final concentration OD600 of the saccharomyces cerevisiae and the final concentration OD600 of the sporospora delbrueckii are 0.8-1.5, 0.8-1.5, 0.5-1, 0.8-1.5 and 0.5-1 respectively. After the compound microbial agent provided by the invention is applied to a solid-state fermentation system of Luzhou-flavor liquor, the yield of the aroma substance beta-phenethyl alcohol can be obviously increased by adjusting the eukaryotic microbial community structure of the fermentation system; the method can effectively solve the problem that the stability of the method for producing beta-phenethyl alcohol in the process of intensifying Baijiu fermentation by a single strain is poor.

The invention relates to the related technical field of extraction of active ingredients in roselle calyx, and particularly provides an extract based on roselle oil and roselle polypeptide as well as an extraction method and application of the extract. The extraction method comprises the following steps: S1, sequentially performing crushing treatment and supercritical fluid extraction treatment on roselle calyx to obtain a roselle oil extract and flower residues; s2, the roselle residues are sequentially subjected to fermentation treatment and enzymolysis treatment, and a roselle polypeptide extract is obtained; wherein a strain used in the fermentation treatment is selected from at least one of saccharomyces cerevisiae, bacillus subtilis and lipomyces starkeyi; an enzyme used in the enzymolysis treatment is selected from at least one of acid protease and compound protease; the extract comprises the roselle oil extract and the roselle polypeptide extract. The extraction method provided by the invention improves the extraction rate of active ingredients extracted from roselle calyx.

The invention relates to a gene modified cell and a method for producing fucosyllactose by using the gene modified cell, in particular to a saccharomyces cerevisiae gene modified cell and application thereof, and belongs to the technical field of biological gene engineering. The present invention provides a genetically modified cell comprising a recombinant nucleic acid sequence encoding a polypeptide having an [alpha]-1, 2-fucosyltransferase polypeptide ([alpha]-1, 2-fucosyltransferase) activity. The alpha-1, 2-fucosyltransferase polypeptide includes, but is not limited to, polypeptides having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical amino acid sequences with the amino acid sequences of SEQ ID NO: 1-25. According to the gene modified cell, the fucosyllactose synthesis capability of an original strain is improved.

The invention provides an application of an MET14 gene or an MET14 protein in improving the freezing resistance of saccharomyces cerevisiae. It is found for the first time that through overexpression of the MET14 gene or the MET14 protein, the freezing resistance of the saccharomyces cerevisiae can be remarkably improved, the saccharomyces cerevisiae can normally ferment and grow in frozen products, the excellent physiological activity of the saccharomyces cerevisiae in the frozen products is fully exerted, and then the quality of the frozen products is remarkably improved.

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention discloses a ruminant feed capable of replacing soybean meal as well as a preparation method and application of the ruminant feed. The ruminant animal feed is prepared from the following raw material matrixes in parts by weight through compound probiotic fermentation: 45-55 parts of corn sugar residues, 15-25 parts of corn steep liquor, 10-20 parts of corn germ meal, 3-8 parts of extruded soybeans, 3-8 parts of corn protein powder, 2-4 parts of urea and 0.5-1.5 parts of lysine. The compound probiotics are prepared from saccharomyces cerevisiae, lactobacillus plantarum and bacillus subtilis. The invention further discloses a preparation method of the ruminant feed and application of the ruminant feed in completely replacing soybean meal in daily ration of ruminants. The ruminant feed disclosed by the invention can completely replace soybean meal in daily ration of ruminants, and even if the ruminants are in a fattening period, the soybean meal does not need to be additionally added to maintain protein supply, so that the feed cost is effectively reduced, and multiple targets of saving cost, improving efficiency and protecting environment are achieved.

The invention relates to the technical field of biology, in particular to application of N-terminal truncated 8-isopentenyl transferase in production of 8-isopentene kaempferol. The invention provides the N-terminal truncated 8-isopentenyl transferase, and the N-terminal truncated 8-isopentenyl transferase can be used for carrying out isopentenylation on kaempferol to generate 8-isopentene kaempferol. Specifically, the saccharomyces cerevisiae is used as a chassis, under the condition that kaempferol is exogenously added, through overexpression of 8-isopentenyl transferase, biosynthesis of 8-isopentenyl kaempferol in the saccharomyces cerevisiae is realized, and meanwhile, a foundation is laid for subsequent biosynthesis of icaritin. The invention provides a novel reference scheme for biosynthesis of flavonoid compounds.

The invention relates to the technical field of fruit wine, in particular to herbal fresh fruit wine and a brewing process thereof. The preparation method comprises the following steps: juicing juicy peaches, apples and raspberries, and adding water for blending; the preparation method comprises the following steps of: adding cellulase and pectinase for primary enzymolysis, adding papain and bromelain for secondary enzymolysis, performing enzyme deactivation and sterilization, inoculating bacillus licheniformis CI CC 24553, lactobacillus plantarum CI CC 21794 and saccharomyces cerevisiae CI CC 1406 for fermentation, and performing post-treatment to prepare the herbal fresh fruit wine. The herbal fresh fruit wine prepared by the invention has the effects of excellent oxidation resistance, high total sugar content and excellent smell and taste, and has better use experience.

The invention relates to the technical field of microbial genetic engineering, in particular to a method for improving cordycepin synthesized by saccharomyces cerevisiae and saccharomyces cerevisiae engineering bacteria. According to the invention, codon-optimized 2 '-carbonyl-3'-deoxyadenosine reductase gene and 3 '-adenosine monophosphate phosphohydrolase gene from cordyceps militaris are expressed by using plasmids, and then the plasmids are transformed into host cells to obtain the saccharomyces cerevisiae engineering bacteria. And inoculating a seed solution obtained by seed culture of the activated saccharomyces cerevisiae engineering bacteria into a fermentation culture medium containing a metabolic effector, and carrying out fermentation culture to synthesize cordycepin. Metabolic effectors (Cu < 2 + >, Fe < 2 + >, Mg < 2 + >, Zn < 2 + >, citric acid, cysteine, aspartic acid, glycine, VB1 and tea polyphenol) are added into a fermentation culture medium strain, and the variety and concentration of the metabolic effectors are optimized, so that the yield and synthesis efficiency of cordycepin are remarkably improved. By using the combination of the strain obtained by the invention and the optimal effector, the yield of cordycepin in a fermentation tank reaches 2.9 g / L.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus paracasei ZY and application thereof, and the preservation number of the lactobacillus paracasei ZY is CGMCC (China General Microbiological Culture Collection Center) No.30303. The invention provides the lactobacillus paracasei ZY capable of regulating the iron balance of intestinal tracts, and the lactobacillus paracasei ZY also can promote the growth of saccharomyces cerevisiae. The animal feed prepared from the lactobacillus paracasei ZY can regulate the iron absorption of intestinal tracts according to the iron content in blood, promote the intestinal tracts to autonomously regulate the iron balance, and effectively solve the problem of iron balance imbalance in large-scale production; a fermented product prepared from the lactobacillus paracasei ZY is good in palatability, digestion is promoted, and the immunity of the organism is enhanced.

The invention discloses a breeding method of high-concentration cane molasses tolerant robust saccharomyces cerevisiae and high-concentration ethanol fermentation application of the high-concentration cane molasses tolerant robust saccharomyces cerevisiae, and belongs to the technical field of biology. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on July 25, 2023, and the number of the Saccharomyces cerevisiae NGTM-F1 is GDMCC 63687. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is named as Saccharomyces cerevisiae NGTM-F1. The saccharomyces cerevisiae is obtained by taking laboratory saccharomyces cerevisiae GJ08 as an original strain, carrying out ARTP (room temperature plasma) mutagenesis and adaptive evolution breeding and utilizing high-throughput screening, keeps a good growth state in cane molasses with the total sugar being 300 g / L, and has good fermentation performance in cane molasses with the total sugar being 250 g / L at the temperature of 30 DEG C. Under the condition of 30 DEG C in a conical flask, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 102.30 g / L, and the fermentation efficiency is 80.8%; under the condition of 30 DEG C in a 5 L fermentation tank, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 111.65 g / L, and the fermentation efficiency is 87.0%.

The invention relates to a diacylglycerol acyltransferase mutant and application thereof in synthesis of triacylglycerol from saccharomyces cerevisiae, and belongs to the technical field of enzyme engineering. Diacylglycerol acyltransferase is a rate-limiting step of a synthetic route of saccharomyces cerevisiae triacylglycerol (TAG), and at present, a research on a DGA1 mutant for efficiently synthesizing TAG is lacked, and a research on the aspect of a DGA1 catalytic mechanism is also lacked. The DGA1 mutant capable of effectively improving TAG synthesis is obtained by screening in combination with a directed evolution technology, and a result shows that 282-site mutation of diacylglycerol acyltransferase can significantly increase the accumulation amount of TAG. Meanwhile, the catalytic mechanism of the DGA1 is preliminarily explored by combining protein structure prediction, molecular docking and molecular dynamics simulation, and a foundation is laid for deeply analyzing the catalytic mechanism of the DGA1. The recombinant saccharomyces cerevisiae is also constructed based on the mutant, so that the TAG synthesis is improved, and meanwhile, the relative proportion of C18: 1 in total fatty acids is remarkably increased.

The invention discloses corynebacterium glutamicum for producing N-acetylglucosamine and application of the corynebacterium glutamicum, and belongs to the technical field of synthetic biology. Specifically, the corynebacterium glutamicum overexpresses a GNA1 gene from a brewer's yeast S288C controlled by a tacM promoter, a glmS gene from corynebacterium glutamicum S9114 and a yqab gene from escherichia coli K12 MG1655, and simultaneously has five gene defect types of nagA, nagB, ldhA, msccg and msccg2 in a genome and two gene single base mutation types of odhA and glnA, and the codon of the glnA for coding the 405th amino acid is mutated from TAC to TTC. By blocking excretion of glutamic acid of corynebacterium glutamicum and enhancing conversion of glutamic acid to glutamine, the supply capacity of a precursor of corynebacterium glutamicum is enhanced, and an optimal way for synthesizing N-acetylglucosamine is obtained, so that efficient production of N-acetylglucosamine in corynebacterium glutamicum is realized, and the yield of N-acetylglucosamine in corynebacterium glutamicum is increased. The method has important economic value and social significance.

The invention relates to saccharomyces cerevisiae capable of producing soluble beta-glucan at high yield and application of the saccharomyces cerevisiae. According to the invention, the saccharomyces cerevisiae with high yield of soluble beta-glucan is obtained by screening in a manner of separating and screening from soil. The saccharomyces cerevisiae MF-001 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center on January 3, 2025, and the preservation number is GDMCC No: 65715. The saccharomyces cerevisiae MF-001 obtained through screening and separation can reach the logarithmic phase more quickly, the fermentation period is short, the fermentation efficiency is improved, meanwhile, the saccharomyces cerevisiae MF-001 has the high beta-glucan yield, a liquid product which is high in purity, colorless, transparent, low in viscosity and high in flowability can be obtained through the fermentation liquor obtained through fermentation of the saccharomyces cerevisiae MF-001 through a simple purification step, and the yield of beta-glucan is improved. The subsequent purification production efficiency can be improved. And the liquid product is proved to have good biological activity and wide application prospect.

The invention discloses a kind of eucommia leaf fermentation extract and its preparation method and application, and belongs to the technical field of microorganisms and feed additives. With eucommia leaf and wheat bran as substrates, by configuring a specific black aspergillus niger-trichoderma harzianum-saccharomyces cerevisiae complex bacterial liquid as a fermentation agent, a closed fermentation is carried out to make black aspergillus, trichoderma harzianum, and saccharomyces cerevisiae ferment to produce a large amount of cellulase, protease, lipase, amylase and glucosidase, etc., and then by pumping and circulating treatment, the cellulose, protein, starch, beta-glycan and other macromolecular substances in eucommia leaf are further enzymolyzed into protein oligopeptides, monosaccharides, and soluble oligosaccharides that are beneficial to livestock and poultry absorption. The eucommia leaf fermentation extract prepared by the present invention is used as a feed additive for beef cattle feeding, improves the daily weight gain and feed intake of beef cattle, and significantly reduces the feed-to-meat ratio, saves feed consumption, improves the protein content in meat, reduces the fat content, reduces energy, and improves meat quality.

The invention relates to the technical field of mulberry processing, in particular to fermented mulberry powder with sleep-aiding and sugar-resisting effects as well as a preparation method and application of the fermented mulberry powder. The preparation method comprises the following steps: adding mulberries into water, and pulping to prepare pulp; adding a compound enzyme into the slurry for enzymolysis to obtain an enzymatic hydrolysate; equally dividing the enzyme solution into two parts, and respectively adding saccharomyces cerevisiae and plant lactobacillus for first-stage fermentation to obtain a first fermentation solution; sterilizing the first fermentation liquor, and adding casei paracasei for second-stage fermentation to obtain second fermentation liquor; and filtering the second fermentation liquor, adding a solid filler into the filtrate, and then sterilizing and drying in sequence to obtain the fermented mulberry powder. Compared with common mulberry powder, the fermented mulberry powder prepared by the preparation method disclosed by the invention has the effects of aiding sleep and resisting sugar, is obvious in aiding sleep, can inhibit generation of AGEs, and has the effect of inhibiting glycosylation.

The invention discloses a rare ginsenoside dammadienol synthetase and an application of the rare ginsenoside dammadienol synthetase in preparation of dammadienol. The dammadienol synthetase is identified for the first time in the research, a metabolic network of a saccharomyces cerevisiae host cell is modified through a metabolic engineering strategy, genes which do not exist in the host cell are introduced into an engineering strain for heterologous expression, the host cell is changed from nothing to existence, and the dammadienol engineering strain is constructed. In addition, through homologous modeling, key catalytic pocket searching and key enzyme gene site-directed mutagenesis modification, enzyme catalysis efficiency is improved, efficient synthesis of dammadienol is achieved, the problem that natural sources of the rare ginsenoside are insufficient is solved, green synthesis and sustainable utilization of the rare ginsenoside are promoted, and the method is suitable for industrial production. And a foundation is laid for subsequent construction of rare ginsenoside cell factories.

The invention belongs to the technical field of biological fermentation and cosmetics, and particularly relates to a cortex magnoliae officinalis fermented product with oil-controlling and dandruff-removing effects as well as preparation and application of the cortex magnoliae officinalis fermented product. The preparation method comprises the following steps: performing ethanol ultrasonic extraction on officinal magnolia bark powder to obtain an officinal magnolia bark extract; adding mangnolia officinalis, honeysuckle, green tea and pericarpium citri reticulatae powder into pure water, performing enzymolysis treatment by adopting cellulase and pectinase, then adding a carbon source, prebiotics and inorganic salt, performing high-temperature sterilization, performing cooling, then adding a mangnolia officinalis extract, and performing uniform mixing so as to obtain a fermentation culture medium; the method comprises the following steps: inoculating an aspergillus seed solution and a saccharomyces cerevisiae seed solution into a fermentation culture medium for first-step fermentation, heating and inactivating, cooling, inoculating fruit wine yeast for second-step fermentation, and sterilizing at high temperature, cooling and filtering after fermentation to obtain filtrate which is the cortex magnoliae officinalis fermented product with the effects of controlling oil and removing dandruff. According to the preparation method, enzymolysis and fermentation technologies are combined, and the honeysuckle, the green tea and the pericarpium citri reticulatae are fermented together, so that the antibacterial, anti-dandruff and oil-control effects of the mangnolia officinalis can be synergistically improved.

The invention belongs to the field of bioengineering, and particularly relates to phosphorylcholine cytidyltransferase and application thereof in preparation of citicoline, and the specific application is that the phosphorylcholine cytidyltransferase can efficiently catalyze phosphorylcholine and CTP to react to generate citicoline. The phosphorylcholine cytidyltransferase is prepared by the following steps: synthesizing a coding gene of the phosphorylcholine cytidyltransferase, selecting pHT01 as an expression vector, co-expressing with choline kinase, transforming into host bacteria, and inducing protein expression. According to the choline phosphate cytidyltransferase disclosed by the invention, under the same reaction condition and within the same reaction time, the yield of a citicoline product obtained by conversion is higher than that of choline phosphate cytidyltransferase (ScCCT) which is most commonly used in the market at present and is derived from saccharomyces cerevisiae S288c. Specifically, the yield of the MsCCT001 is 10 times higher than that of the ScCCT, the yield of the MbCCT006 is 6 times higher than that of the ScCCT, and the yield of the MeCCT008 is 8 times higher than that of the ScCCT.

The invention particularly relates to a saccharomyces cerevisiae genetically engineered bacterium for de novo synthesis of 7-DHC as well as a construction method and application of the saccharomyces cerevisiae genetically engineered bacterium and belongs to the technical field of biology. According to the invention, a saccharomyces cerevisiae engineering bacterium SC9 is taken as an original strain, multiple times of multiple copies of tHMG1, DHCR24 and ERG1 are carried out on TY transposon, multiple copies of ERG2, POS5, ERG7, ERG11, IDI1 and ERG3 are carried out on TY transposon, and POS5, ERG3 and DHCR24 are singly copied on a genome, so that the content of intracellular reducing coenzyme II is increased, and a main path for synthesizing 7-DHC is enhanced. The genome single copy ACL, ACS1, ERG10, ERG19 and the like are used for enhancing the utilization of saccharomyces cerevisiae ethanol and dynamically regulating and controlling the synthesis of 7-DHC. The saccharomyces cerevisiae gene engineering strain constructed by the invention has high product accumulation, and lays a foundation for replacing a traditional multi-step chemical synthesis route and realizing industrial production.

The invention belongs to the technical field of biology, and particularly relates to a preparation method and application of a rose extract, and the content of water-soluble vitamins in roses is increased by utilizing a microbial fermentation technology. According to the method, strains including lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are selected, the proportion of the strains is adjusted, the viable count proportion of the lactobacillus plantarum to the saccharomyces cerevisiae to the bacillus subtilis is (1-3): (1-2): (1-2), a complex microbial inoculant is formed, and pretreated roses are added for segmented fermentation. And finally, the content of water-soluble vitamins in the rose extract is increased, so that the total antioxidant activity is increased by about 35% while rose polysaccharide substances are retained. The rose extract is suitable for providing raw materials for functional food, health care products and cosmetics.

The invention discloses a medicinal and edible homologous compound based on probiotic fermentation synergy and application of the medicinal and edible homologous compound in blood sugar regulation and control, and belongs to the technical field of microorganisms and fermentation engineering. The preparation method comprises the following steps: respectively carrying out two-stage fermentation on aloe, mulberry leaves and liquorice by utilizing saccharomyces cerevisiae BLCC4-0032 and plant lactobacillus DFR-1, so as to obtain a fermentation mixture; the preparation method comprises the following steps: fermenting a fermentation mixture, adding balsam pear peptide into the fermentation mixture to obtain a medicinal and edible compound, forming a metabolic closed loop, and cooperatively regulating a'blood sugar-liver-intestinal flora axis', thereby providing a new solution for diabetes intervention, and opening up a new path for preventing non-alcoholic fatty liver and intestinal inflammation caused by glucose and lipid metabolism disorder.

The invention belongs to the technical field of biological control, and particularly relates to biological control fermentation liquor and a preparation method and application thereof, the preparation method comprises the following steps: S1, raw material treatment: selecting solidago canadensis as a plant raw material, cutting the solidago canadensis into pieces, performing autoclaved sterilization, adding water, and mixing to obtain a mixture; s2, adding the cultured bacillus subtilis and saccharomyces cerevisiae into the mixture in the step S1, mixing and inoculating, and stirring and fermenting to obtain a fermentation stock solution; s3, centrifuging the fermentation stock solution, taking supernate, and filtering to obtain the biological control fermentation liquor. The biological control fermentation liquor prepared by the invention can be used for controlling seed germination, seedling growth and flowering processes of solidago canadensis, and meanwhile, the biological control fermentation liquor can also be used for preparing products for controlling solidago canadensis. The problem that a biological medicament for preventing and treating solidago canadensis is lacked at present is solved.

The invention relates to a preparation method of traditional Chinese medicine residue metabiotics and application of the traditional Chinese medicine residue metabiotics in fish feed, and belongs to the technical field of feed additives. Beneficial bacteria such as bacillus subtilis, lactobacillus lactis, bifidobacterium longum and saccharomyces cerevisiae are separated from intestinal contents of grass carp and are subjected to enlarged culture, then traditional Chinese medicine residues such as fresh ginger, folium mori, ginseng, fructus lycii, rhizoma curcumae longae, liquorice and mint are added, staged mixed fermentation culture is performed, obtained metabolites are added into fish feed, and the fish feed is obtained. The feeding rate and the growth rate of fishes such as mandarin fish and micropterus salmoides are effectively improved, and the resistance is enhanced.

The present application relates to the technical field of biocatalysis and biosynthesis, and specifically discloses a natural blue pigment and a biosynthesis method therefor. The present application uses metabolically engineered bacteria to express indigoidine synthetase and phosphopantetheinyl transferase, to catalyze glutamine and N-acetylglutamine to biosynthesize a natural blue pigment N-acetylindigoidine, and deduces its molecular structure by means of mass spectrometry, nuclear magnetic resonance and other methods. The present application achieves catalytic synthesis of N-acetylindigoidine from glutamine and N-acetylglutamine in Escherichia coli, Corynebacterium glutamicum, Saccharomyces cerevisiae and Streptomyces. Compared with indigoidine, N-acetylindigoidine has a maximum absorption wavelength of 584 nm, possesses better color brightness, and has more stable coloration that is not easily faded, with a very broad application range and industrial production application prospects.

The invention provides a high-yield GPP / FPP saccharomyces cerevisiae engineering strain as well as a construction method and application thereof, and relates to the technical field of synthetic biology. Through a gene editing technology, heterologous mvaE and mvaS genes are introduced into saccharomyces cerevisiae, endogenous ERG12, ERG8, ERG19 and ERG20 genes are over-expressed, and the metabolic flux of an MVA pathway in saccharomyces cerevisiae cells is enhanced; meanwhile, a positioning signal tag of peroxisome is added to the gene, and an MVA pathway and a terpenoid precursor synthesis pathway are reconstructed in the peroxisome, so that the compartment synthesis of the terpenoid precursor is realized. Besides, a heterologous MthIPKs gene is further introduced, a branch pathway for IPP synthesis is constructed, higher energy efficiency and less enzymatic reactions are achieved, an MVA pathway and metabolic flux for terpenoid synthesis are concentrated, and the biosynthesis efficiency and conversion rate are further improved.

The invention discloses saccharomyces cerevisiae MBPSc21 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc21 for expressing immune nutrient-beta-carotene, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc21 is CGMCC No.32663; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene, and the content of the beta-carotene is reduced. The soybean meal protein is endowed with new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and a leading level is achieved in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention relates to the technical field of Chinese herbal medicine fermentation, and discloses a mixed strain fermentation method for efficient conversion of non-medicinal parts of traditional Chinese medicine, which comprises the following steps: constructing an intelligent fermentation system comprising a plurality of independent fermentation units, respectively culturing different strains such as aspergillus niger, bacillus subtilis, saccharomyces cerevisiae and the like in each fermentation unit, the online sensor is integrated in the system and used for monitoring key fermentation parameters in real time, and a fermentation model and an optimization algorithm are arranged in the system and can automatically adjust fermentation conditions. An intelligent fermentation system comprising a plurality of independent fermentation units is constructed, precise classification pretreatment and mixing according to a specific ratio are carried out on rhizoma polygonati stems and leaves and gentian stems and leaves, and an intelligent control system is utilized to dynamically regulate and control the fermentation process, so that the synergistic effect of different strains such as aspergillus niger, bacillus subtilis and saccharomyces cerevisiae is fully exerted, and the fermentation efficiency is improved. The dissolution rate and the conversion rate of active ingredients in the non-medicinal part of the traditional Chinese medicine are improved.

The invention belongs to the technical field of biology, and discloses a yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application of the yarrowia lipolytica engineering strain. Delta9 elongase, Delta8 desaturase, Delta5 desaturase and Delta17 desaturase genes in a fatty acid synthesis route, heterologous ATP-citrate lyase and a mitochondrial citric acid vector are overexpressed. According to the invention, ATP-citric acid lyase and a mitochondrial citric acid carrier gene which are respectively derived from Schizochylene sp. And Saccharomyces cerevisiae are jointly expressed in the yarrowia lipolytica engineering strain, so that the yields of EPA and grease in the yarrowia lipolytica engineering strain are remarkably improved, and compared with a control strain, the yields of the grease and the EPA are respectively improved by 243.35% and 161.85%.