The invention proposes beer yeast capable of producing beer with the low acetaldehyde content, and a domestication method of the beer yeast. The beer yeast is DY-01, a preservation number of the beeryeast is CGMCC No. 19840, and the beer yeast is preserved in the China General Microbiological Culture Collection Center on May 19, 2020. The domestication method mainly comprises the steps such as adaptive evolution treatment, domestication treatment, enzyme labeling determination, and fermentation result evaluation and screening. The domestication method of the invention does not perform drasticmutagenesis on the strain, and does not produce drastic changes to the strain's genome, but an adaptive evolution method is adopted, and an alcohol dehydrogenase repressor and an acetaldehyde dehydrogenase repressor are added into a culture medium, so that activities of both alcohol dehydrogenase (ADH2) and acetaldehyde dehydrogenase (ALDH) of the beer yeast are improved, and the ability that acetaldehyde is metabolized into ethanol and acetic acid is further improved. The beer yeast and domestication method have broad application prospects in the field of beer yeast and a domestication method thereof.