170 results about "Biological studies" patented technology

The invention provides a construction method of a predictive cell senescence model, a method for predicting the senescence state of a tissue sample based on the senescence model, a method for screening potential therapeutic drugs, equipment, a medium and a program product, and relates to the field of intelligent medical treatment. The model construction method comprises the following steps: acquiring a training set sample expression profile data set; identifying a key senescence gene set from the data set by using a feature selection algorithm; inputting the key senescence gene set into a machine learning model to fit a prediction model, and determining an optimal hyper-parameter to obtain a cell senescence model containing the weight of a single gene in the key senescence gene set; the cell senescence model is a senescence score obtained by calculating the sum of the product of the expression quantity of a single gene and the regression coefficient thereof. The cell senescence model, namely PreCSenM, is constructed by integrating a plurality of senescence characteristic gene sets and a gene scoring algorithm, the accuracy in CS evaluation is superior to that of 10 existing methods, and the application of CS from biological research to clinical scenes is also realized.

The invention is applicable to the technical field of gene engineering, and provides an andrographis paniculata endogenous cis-regulatory element ApACR1 and application thereof.The nucleotide sequence of the cis-regulatory element ApACR1 has transcriptional activity equivalent to that of a 35S promoter, and the cis-regulatory element ApACR1 can replace the 35S promoter to be used as an andrographis paniculata endogenous regulatory element. Some potential risks of the 35S promoter in molecular breeding are avoided, and the promoter has important potential application value in subsequent andrographis paniculata molecular biology research and molecular breeding.

The invention discloses an application of fangchinoline alkali in preparation of a product for inducing aging of tumor cells. The invention provides a novel application of fangchinoline alkali in induction of tumor cell senescence, which is beneficial to deep understanding of biological behaviors of tumor cells. By using the fangchinoline alkali as the tumor cell aging inducer, the aging process and the biological mechanism of tumor cells can be effectively simulated in an in-vitro or in-vivo model, so that a powerful tool is provided for biological research.

The utility model provides a double-emulsification high-flux micro-fluidic chip. The double-emulsification high-flux micro-fluidic chip comprises a substrate, a middle layer and a cover plate, according to the utility model, the substrate is not processed, the main micro-channels are processed on the two sides of the middle layer, and the cover plate is punched, so that fluid can be conveniently introduced. A plurality of liquid drop generation units based on the flow focusing technology are designed on one side of the middle layer so as to generate liquid drops for the first time, and a stepped liquid drop generation structure based on interfacial tension is designed on the other side of the middle layer so as to generate liquid drops for the second time. The structures on the two sides of the chip are connected through the through holes. The preparation method comprises the following steps: carrying out photoetching to form a mold, preparing the middle layer by using the mold, forming the required micro-channels on the two sides of the middle layer, punching on the cover plate, and finally bonding the substrate, the middle layer and the cover plate. Through the design of the flow channel and the structure, a large number of double-emulsion droplets can be rapidly prepared, the production efficiency is improved, and the double-emulsion droplet preparation device is suitable for various applications such as drug delivery and biological research.

The invention relates to an odor molecule and olfactory receptor reaction prediction method and device based on a multi-mode deep learning network, and the method comprises the steps: firstly, extracting physical and chemical descriptors of odor molecules as the characteristics of the physical and chemical descriptors through the interaction data between the odor molecules and olfactory receptors, and employing a deep learning network model to predict the reaction between the odor molecules and the olfactory receptors; the method comprises the following steps: firstly, judging whether an unknown odor molecule can excite an olfactory receptor or not, secondly, judging the possibility of reaction between the odor molecule and each olfactory receptor through a network calculation model by adopting odor molecule sequence data, and then, further introducing odor molecule structure image data, and calculating the reaction probability between the odor molecule and each olfactory receptor. And finally, in order to improve the accuracy and reliability of a recognition result, determining a final reaction olfactory receptor of the odorant molecule through a calculation result obtained through analysis, namely two groups of probability distributions. According to the method, the recognition accuracy of the odor molecules and the olfactory receptors is remarkably improved, meanwhile, the dependence on an experimental method is reduced, and a new tool is provided for chemical and biological research.

The invention discloses an oscillation microbubble chip, a separation system and an acoustic manipulation method, the device utilizes the acoustic eddy current effect induced by oscillation microbubbles to realize micro-nano particle acoustic manipulation, the chip structure and operation are simple, the biocompatibility is high, and by designing the chip structure, due to the breathable and water-repellent characteristics of PDMS and the surface tension of fluid, the micro-nano particle acoustic manipulation can be realized. Alternatively arranged equilateral triangle column cavities with different sizes are formed on the side wall of the PDMS microfluidic channel to anchor bubbles. Bubble oscillation is driven by bulk acoustic waves generated by exciting a piezoelectric transducer through a signal generator, symmetrical vortexes are formed on the surfaces of the bubbles through secondary acoustic radiation force generated by bubble oscillation, then large-size particles are captured, and small-size particles flow to an outlet along with flowing under the action of drag force. Gradient separation is adopted to separate small particles from a mixed solution and enrich large particles, enrichment, separation and other control of microparticles are achieved through the acoustic vortex field effect of a specific structure, great potential is achieved in biological research, the structure is simple, the equipment cost is low, and integration is easy.

The invention provides a double-emulsification high-flux micro-fluidic chip and a preparation method thereof. The chip comprises a substrate, a middle layer and a cover plate. According to the invention, the substrate is not processed, but the main micro-channels are processed at the two sides of the middle layer, and the cover plate is punched, so that the fluid can be conveniently introduced. A plurality of liquid drop generation units based on the flow focusing technology are designed on one side of the middle layer so as to generate liquid drops for the first time, and a stepped liquid drop generation structure based on interfacial tension is designed on the other side of the middle layer so as to generate liquid drops for the second time. The structures on the two sides of the chip are connected through the through holes. The preparation method comprises the following steps: carrying out photoetching to form a mold, preparing the middle layer by using the mold, forming the required micro-channels on the two sides of the middle layer, punching on the cover plate, and finally bonding the substrate, the middle layer and the cover plate. Through the design of the flow channel and the structure, a large number of double-emulsion droplets can be rapidly prepared, the production efficiency is improved, and the device is suitable for various applications such as drug delivery and biological research.

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The invention provides a nucleic acid releasing agent and a use method, and relates to the technical field of biological research, by screening main influence factors of the nucleic acid releasing agent and optimizing the concentration, the nucleic acid releasing agent can fully release nucleic acid of pathogens in a clinical sample while ensuring that the nucleic acid releasing agent does not generate an inhibition effect on a subsequent detection system, and the detection accuracy is improved. The nucleic acid releasing agent provided by the invention can be directly used for sampling and storage, and can be stably stored for 3 days at room temperature and 7 days at 2-8 DEG C according to stability research, and the nucleic acid releasing agent can be used for subsequent detection, so that the detection sensitivity is improved. The nucleic acid releasing agent can efficiently release nucleic acid and ensure the quality and quantity of nucleic acid extraction by accurately adjusting the concentration and proportion of each component, a reliable basis is provided for subsequent detection and analysis, accurate diagnosis and research are facilitated, Tris-Cl in the nucleic acid releasing agent controls the pH value within a proper range, the natural structure and function of nucleic acid are kept, and the nucleic acid releasing agent has good application prospects. And degradation and denaturation are reduced.

The invention relates to an osmanthus protoplast preparation method and an instantaneous conversion method. The preparation method of the sweet-scented osmanthus protoplast comprises the following steps: collecting different tissues of fresh sweet-scented osmanthus, performing surface disinfection and sterilization, cutting into thin strips or slices, adding enzymatic hydrolysate for enzymolysis, filtering, centrifuging, washing with a W5 solution, and re-suspending. The instantaneous transformation method of the osmanthus protoplast comprises the following steps: adjusting the concentration of the osmanthus protoplast by using an MMG solution, adding plasmids for mixing, adding a PEG solution for transformation incubation, adding a W5 solution for washing and centrifuging, and detecting the instantaneous transformation efficiency of the osmanthus protoplast. According to the method, a high-quality protoplast separation system of the leaves, the stems and the petals of the sweet-scented osmanthus is successfully established, efficient conversion of mesophyll and petal protoplast is realized for the first time, and a reliable technical platform is provided for molecular biology research of the sweet-scented osmanthus.

The invention provides an induction method of sesbania hairy roots, which comprises the following steps: beveling hypocotyl of a sesbania seedling, dipping recombinant agrobacterium rhizogenes in the oblique incision of the seedling on the ground, inserting the seedling into vermiculite, and culturing until a new root system is generated, namely the new root system is a sesbania transgenic root system. The method is simple to operate, low in cost and short in time consumption, avoids tedious sterile operation, provides important technical support for molecular biology research of sesbania, and has a relatively great application prospect.

The application discloses a covalent polypeptide inhibitor targeting protein kinase A, a conjugate, a kit, a pharmaceutical composition and application. The general formula of the covalent polypeptide inhibitor is Z1-Seq-Z2; wherein Seq is an amino acid sequence; the C-terminal of Seq comprises a pseudo-substrate recognition motif recognized by protein kinase A, a non-natural amino acid with a covalent reaction group, and can be covalently crosslinked with a Cys200 residue on a catalytic subunit of protein kinase A; Z1 is selected from hydrogen, an acyl group, a fluorescent group, biotin, an isotopic tag or a biological ortho-reaction group; and Z2 is selected from a hydroxyl group, an amino group, a cell-penetrating peptide or a stabilization modification group. The covalent polypeptide inhibitor can strongly and irreversibly inhibit PKA activity, and can be used as an active probe to specifically label active PKA in a complex biological sample, and is used for biological research and drug development.

The invention discloses a method for extracting sphagnum moss protoplast, which comprises the following steps of: culturing a sphagnum moss material obtained by tissue culture under a certain condition, and performing pretreatment and enzymolysis under a specific permeation condition to finally obtain the high-quality sphagnum moss protoplast. The method provided by the invention provides an important technical basis for molecular biology research of sphagnum moss transgenosis and the like.

The invention relates to the technical field of biological research data analysis, and discloses a computational biological research data analysis display method, which comprises the following steps of: extracting total RNA (Ribonucleic Acid) from biological tissues or cells, reversely transcribing the RNA into cDNA (Complementary Deoxyribonucleic Acid), constructing a library, and sequencing the library by utilizing a high-throughput sequencing platform to obtain a large number of short sequences. According to the method, the number of the short sequences mapped to the same genome coordinate interval is compared and the threshold value is judged, so that the abnormal repeated area can be effectively identified, whether the gene expression difference result is credible or not is clearly judged, and when the number of the abnormal sequences exceeds the preset repeated sequence threshold value, the influence operation of the repeated sequences is eliminated, and the accuracy of the gene expression difference result is improved. That is to say, completely identical and overlapped short sequences are removed under the condition that the FPKM value is relatively high, and the updating result is compared again, so that the problem of expression quantity distortion caused by repeated reckoning can be corrected.

The invention discloses a cyclic dinucleotide analogue containing a triazole structural unit as well as a preparation method and application of the cyclic dinucleotide analogue. The cyclic dinucleotide analogue containing the triazole structural unit is obtained by taking a macrocyclic ribose phosphate skeleton containing a diazido group as a substrate and expanding the macrocyclic ribose phosphate skeleton and an aromatic alkynyl compound through a click chemistry method, has relatively good biofilm formation inhibition activity, has a potential application prospect, and can be widely applied to biofilm biofilm formation inhibition. According to the invention, a cyclic dinucleotide analogue library is enriched, and biological research of cyclic dinucleotide and development of immunotherapy are promoted.

This invention provides a method for storing mango seeds, belonging to the field of seed preservation technology. The method involves cutting off mango fruits with embryos, along with their pedicels and branches, 77-84 days after fruit set. A mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed on the fruit, pedicels, and branches. After drying, styrene-butadiene latex is applied to the pedicels and branches. Once the latex dries, a mixture of guanidine-copper acetate and benzoyl-pyraclostrobin is sprayed on the fruit, pedicels, and branches again. After drying, the mango fruits with petioles and branches are placed in a sealed box and stored at 10-14°C. The mango variety used is the Golden Phoenix mango. This invention increases the storage period of Golden Phoenix mango seeds from 1.5 months to 5 months, which is of great significance for the genomics and cell biology research of Golden Phoenix mangoes.

The invention belongs to the technical field of microbial genetic transformation, and particularly relates to a method for high-yield production of pleurotus ostreatus transformants, a genetic transformation system and application of the genetic transformation system. Through optimization of protoplast preparation conditions, the number of the obtained protoplast is 4.95 * 10 < 8 > CFU.mL <-1 >, and the regeneration rate of the protoplast in an OM-YEPS regeneration culture medium is 12.7%. A method for producing high-yield pleurotus ostreatus transformants is researched by using a PEG-mediated protoplast transformation method, a set of efficient and stable genetic transformation and screening method is established, about 200-300 positive transformants with stably expressed exogenous genes are obtained, and the transformation efficiency is 65.38%. The genetic transformation system of the high-yield Pleurotus ostreatus transformant, constructed by the invention, lays a solid technical foundation for subsequent gene function verification, metabolic engineering transformation and molecular breeding with excellent characters, and further accelerates the biological research and industrial application process of Pleurotus ostreatus and even other macro fungi.

The invention discloses an in-situ sampling method for root exudates of sesbania cannabina cultured by a solid substrate, and belongs to a technology in the related field of plant metabonomics. The method comprises the following steps: culturing sesbania in a solid matrix, obtaining a complete sesbania root system attached with rhizosphere soil, extracting root exudates by shaking, centrifuging to take supernatant, freeze-drying and the like. The method is simple to operate and short in time consumption, not only combines the advantages of traditional in-situ sampling to obtain the most real root exudates of sesbania in real time, but also can simulate the influence of saline-alkali stress and the like under natural conditions, is wide in application scene, effectively reduces the acquisition difficulty of the root exudates under the interaction of different soil conditions and plants, and has a wide application prospect. Important technical support is provided for biological research of sesbania, and the method has a relatively great scientific research application prospect.

The invention discloses a method for constructing a transgenic zebrafish model of hypoxia response. The method comprises the following steps: firstly assembling an sv40mp promoter after a hypoxia response element HRE sequence and cloning the promoter into a pT2AL200R150G plasmid carrying green fluorescent protein (GFP), then microinjecting the recombinant plasmid and transposase mRNA into zebrafish embryos, and inserting the recombinant sequence into the genome of F0 generation zebrafish by Tol2 transposon technology; raising the F0 generation fish to adult fish, hybridizing with wild-type zebrafish to produce F1 generation, hybridizing the F1 generation with the wild-type fish again to produce single-copy transgenic zebrafish F2 generation, self-pollinating male and female sibling zebrafish of the F2 generation, and screening out transgenic zebrafish Tg (HRE-sv40mp:GFP) of hypoxia response of F3 generation that can be stably inherited. The transgenic zebrafish Tg(HRE‑sv40mp:GFP) constructed in the present invention can be used as a simple and efficient hypoxia response model for biological research in hypoxia-related fields, as well as for screening molecular targeted drugs for HIF‑1α, providing a living animal screening model for the development of anti-tumor drugs.

The application discloses a monoclonal antibody of a rice field eel Adam8a and a preparation method and application thereof, relates to the technical field of biological antibodies, and fills the research blank of the rice field eel Adam8a. The rice field eel Adam8a monoclonal antibody is high in specificity and titer, simple in preparation process, and controllable in cost, can be widely used for recognizing Adam8a homologous proteins in different fish ovary, and is suitable for being prepared into a fish ovary Adam8a protein detection kit, and is compared with the expression of the protein in different fish, so that the rice field eel Adam8a monoclonal antibody becomes a very convenient detection tool in the biological research of fish ovary development.

The present disclosure, in certain aspects, is directed to the use of causal networks for aspects of biological study and drug discovery, including the identification of biological pathways, or components thereof, associated with a stimulus and / or condition, identification of a drug useful for treating and / or preventing a condition, drug target prioritization, and drug repurposing.

The utility model relates to an automatic tool setting device for a wax block of a slicing machine, which belongs to medical laboratory equipment and is applied to the fields of medical clinical pathological histology, cytology, clinical examination, biological research and the like. The wax block automatic tool setting device has the advantages that the displacement sensor is installed in the middle of the side of the wax block chuck below the blade of the blade frame, automatic tool setting of the wax block is achieved through positioning signals and control of the displacement sensor, fingers are prevented from being cut or scratched in the manual tool setting process of the wax block, the accuracy of tool setting of the wax block is improved, and the speed of tool setting of the wax block is increased; and the practicability, flexibility and reliability are improved. Unnecessary manual operation steps are reduced, simplicity and rapidness are achieved, labor and time are saved, and the working efficiency is improved. Therefore, the method has great market prospect and economic value.

The invention discloses a luciferase mutant, the amino acid sequence of which is as shown in SEQ ID NO. 1, and the polynucleotide sequence of which is as shown in SEQ ID NO. 2. Compared with a wild type, the luciferase mutant disclosed by the invention has the advantages that the catalytic activity is improved by 3 times, and the luciferase mutant can be industrially prepared, can be used for preparing a luciferase detection method kit and can also be used as a reporter protein or a reporter gene for biological research.

The invention relates to ribozyme for labeling biotin on target RNA (Ribonucleic Acid) as well as a screening method and application thereof. The RNA labeling technology is one of core tools for modern molecular biology research and clinical diagnosis. A specific marker is covalently linked to a target RNA molecule, so that accurate tracking, efficient detection and functional analysis of RNA can be realized. A biotin-streptavidin system becomes one of the technical routes which are most widely applied due to extremely high affinity and signal amplification capability of the biotin-streptavidin system. At present, common biotin-labeled RNA technical methods mainly comprise a chemical solid-phase synthesis method, an enzymatic method and a chemical labeling method. However, the methods have the limitations of poor site specificity, low efficiency, complex manipulation and the like in the use process. The engineering ribozyme for catalyzing RNA biotinylation is obtained by taking 12: 0 biotin coenzyme A as a biotin donor through an in-vitro screening technology. The ribozyme provided by the invention can efficiently and specifically carry out biotin labeling on target RNA, the reaction process is simple, and the labeling activity is up to 90% or above.

The present disclosure relates to methods for evaluating the interaction of a candidate compound on 3D hepatocyte spheroid in an invitro culture, including evaluating the metabolism of a candidate compound, for use in various biochemical and molecular biology studies. The methods are performed in labware that combine 3D spheroid culture with micro-patterned design that allows for multiple to several hundreds of spheroids to be treated under the same conditions and to produce sufficient materials (e.g., parent drug, drug metabolites, DNA, RNA, and proteins from cells) and higher detection signal intensity for ADME / Tox (absorption, distribution, metabolism, excretion and toxicity) studies. The methods allow for, among other uses, the investigation and generation of accurate invitro intrinsic clearance data and thus more accurate prediction of in vivo clearance, particularly with low clearance compounds.

The application provides a key enzyme involved in glycosylation modification of triterpene saponins, and an application thereof, and belongs to the technical field of genetic engineering. The glycosyltransferase gene is screened based on the omics research of the base plant of traditional Chinese medicine Duanxueliu, i.e. clinopodium gracile, and a pET28a-MBP-CcGT-g51295 prokaryotic expression vector is constructed to successfully obtain the glycosyltransferase. It is found that the glycosyltransferase can catalyze Saikogenin A and Saikogenin F to generate Prosaikogenin A and Prosaikogenin F respectively by using a glycosyl donor UDP-fucose; can catalyze Saikogenin A to generate clinopodiside I and clinopodiside X by using UDP-glucose; can catalyze Prosaikogenin A to generate Saikosaponin B1, Saikosaponin c1 and Saikosaponin X by using UDP-glucose; and can catalyze Prosaikogenin F to generate Saikosaponin A and Buddlejasaponin IV by using UDP-glucose, which reveals the catalytic function of the glycosyltransferase and provides a gene component for the biological research of triterpene saponins, and solves the problem of biological resources of rare saponins.

The invention relates to a tissue culture technology, in particular to an awnless brome callus induction culture medium and application thereof, and the awnless brome callus induction culture medium is characterized by comprising MS, sucrose, agar, 6-BA and 2, 4-D. The induction is performed by using awnless brome seeds. The awnless brome seed callus is cultivated, a new experimental material is provided for molecular biology and genetic engineering research of awnless brome, and a solid foundation is laid for molecular biology research of awnless brome. Finally, according to the culture medium, the hormone proportion and variety difference are optimized, more calluses can be induced to be generated, the overall induction efficiency is improved, the quality of the calluses can be improved, the growth stability of the seed calluses can be kept through accurately controlled environmental conditions, the difference between batches is reduced, and the yield of the seed calluses is increased. Therefore, the method is more suitable for subsequent awnless brome regeneration or genetic engineering application.

The invention discloses a universal method for producing multi-sample SNP variation sites, and relates to the technical field of biological information. Comprising the following steps: establishing a BWA / samtools index for a reference genome; comparing the sample fastq to generate SAM and converting the SAM to BAM; carrying out sambamba sorting and de-duplication; the method comprises the following steps: adding ReadGroup to Picard; constructing a BAM index; the GATK generates gvcf and converts the gvcf into VCF; the index is compressed by bgzip / tabix (bgzip / tabix); and the MergeVcfs merges and summarizes the VCFs. The simple and general method for the multi-sample SNP variation sites has remarkable advantages in efficiency, accuracy, simple universality, expandability and the like, is expected to play an important role in multiple fields such as biological research, medical diagnosis, agricultural breeding and the like, and promotes technical progress and scientific research development in related fields.

The present invention belongs to the technical field of genetic engineering, and specifically relates to a copepod luciferase mutant and the use thereof. The mutant is obtained by means of performing one or more mutations on a wild-type copepod luciferase with the signal peptide removed, and can be expressed in a prokaryotic expression system, resulting in an increased luminescence half-life or a signal rise within a certain time range, and the luminescence pattern of the luciferase is converted to glow-type luminescence or luminescence with a sustained increase in luminescence intensity. Some copepod luciferase mutants exhibit enhanced luminescence intensity. The mutant is widely used in biological research for real-time tracking and continuous monitoring, such as for labeling and tracking activities or processes in living organisms. On the basis of the advantage of increased luminescence half-life of the copepod luciferase mutant, the mutant can be used as and / or in the preparation of a glow-type luciferase. The copepod luciferase mutant is more suitable as a reporter gene for in-vivo detection in scientific research, and is conjugated with avidin, a digoxin antibody, etc., for sequencing, thereby shortening the sequencing time and improving the sequencing quality.

The utility model relates to the technical field of plant living body imaging, and discloses a plant living body imaging and simulation illumination device which comprises a box body, when the device is used, an LED plant growth lamp can be turned on firstly through an arranged environment simulation assembly, and light irradiates a plant through a through hole; light is dispersed around plants through the astigmatic lenses arranged on the through holes, so that more real natural light is simulated, meanwhile, natural light such as sunny days, cloudy days and night with moonlight can be simulated through the colored lenses, and an environment closer to the natural environment is created. According to the present invention, with the arrangement of the CCD camera, the weak fluorescence signal can be captured, the specific gene of the living plant can be subjected to fluorescence labeling, and after the EMS mutagenesis treatment, the fluorescence brightness change can be detected in the imaging device so as to find the enhancer or the suppressor, such that the biological research data can be obtained; and a better use prospect can be brought.