472 results about "Biological studies" patented technology

Powder type culture medium is from plenty of element kinds , trace element and iron salt and vitamin kind of composition, after processing manufacture the original powder that makes powder culture medium. The various components of the medium and the final product were dry powder. The preparation Method: First, concentrate all plenty of elements to collect reserve; Mix trace element and vitamin kind totally to wrap to attach , and then mix the thin medicine and plenty of elements of trace element totally, under the role with centrifugal high speed, making it even scatter and wrap up to glue to unite. So, make the original powder of powder culture medium for manufacture. This medium-dry powder manufacturing easier, does not require the use of complex machines and equipment, and low-cost easy to promote. The product is of high quality in existing product. It can be used in plant breeding, tissue culture and molecular biology research, and can be used for various commercial purposes.

The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.

A clothes dryer is provided which incorporates ultraviolet light sources to aid in the sterilization of the clothing as it is dried. A series of ultraviolet emitters, envisioned to be germicidal lamps, are located around the perimeter of the drying drum inside of the dryer. As the clothes dryer operates, these lamps are energized, and their light passed thru clear panels into the drying drum where they can aid in the killing of germs, bacteria and the like. Such a feature is envisioned as being particularly useful in hospitals, centers where biological studies are performed, or for those who are particularly susceptible to infectious diseases.

Compositions and methods are provided for integrating one or more genes of interest into cellular DNA without substantially disrupting the expression of the gene at the locus of integration, i.e., the target locus. These compositions and methods are useful in any in vitro or in vivo application in which it is desirable to express a gene of interest in the same spatially and temporally restricted pattern as that of a gene at a target locus while maintaining the expression of the gene at the target locus, for example, to treat disease, in the production of genetically modified organisms in agriculture, in the large scale production of proteins by cells for therapeutic, diagnostic, or research purposes, in the induction of iPS cells for therapeutic, diagnostic, or research purposes, in biological research, etc. Reagents, devices and kits thereof that find use in practicing the subject methods are also provided.

The reconstruction of genetic networks in mammalian systems is one of the primary goals in biological research, especially as such reconstructions relate to elucidating not only common, polygenic human disease, but living systems more generally. The present invention provides novel gene network reconstruction algorithms that utilize naturally occurring genetic variations as a source of perturbations to elucidate the networks. The algorithms incorporate relative transcript abundance and genotypic data from segregating populations by employing a generalized scoring function of maximum likelihood commonly used in Bayesian network reconstruction problems. The utility of these novel algorithms can be demonstrated via application to gene expression data from a segregating mouse population. The network derived from such data using the novel network reconstruction algorithm is able to capture causal associations between genes that result in increased predictive power, compared to more classically reconstructed networks derived from the same data.

The invention discloses a microfluidic chip system integrating cell sorting and detection. The system comprises a microfluidic chip, a high-frequency lock-in amplifier and a processor, wherein the microfluidic chip is formed by aligning and packaging a flow channel layer, an electrode layer, a substrate layer and a PCB (printed circuit board) in sequence; a cell sorting spiral flow channel, a detection main flow channel and a retraction flow channel are formed in the flow channel layer; the retraction flow channel is aligned to a planar metal electrode of the electrode layer; liquid electrode structures are formed on two sides of the main flow channel; an electrode of the electrode layer forms a differential high-frequency impedance measurement circuit together with the high-frequency lock-in amplifier through a power amplification circuit and an I / V conversion circuit of the PCB to realize differential detection of cell alternating-current impedance. The system can realize the integration of rare cell sorting and representation function, improves the integrity and accuracy of a cell detection technology, and can be widely used in the fields of rare cell biological study, early disease diagnosis and treatment and the like.

The membrane scaffold proteins (MSP) of the present invention assemble with hydrophobic or partially hydrophobic proteins to form soluble nanoscale particles which preserve native structure and function; they are improved over liposomes and detergent micelles, in terms of stability and preservation of biological activity and native conformation. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles, which are robust in terms of integrity and maintenance of biological activity of incorporated proteins, facilitate pharmaceutical and biological research, structure / function correlations, structure determinations, bioseparations, and drug discovery.

A method for extracting antineoplastic component from Bupleurum scorzonerifolium applicable in treating cell proliferative disorder is proposed. The antineoplastic components of Bupleurum scorzonerifolium include at least a Y-butyolactone centred heterocyclic compound with a Z configuration or E configuration at its carbon 2(5) position, and molecules, such as Chaihulactone, Isochaihulactone, Chaihulactone analogues or derivatives containing in the heterocyclic compound. From results of cell and molecular biological studies, in vivo animal test, and histological study, it is found that antineoplastic components extracted from Bupleurum scorzonerifolium are effective in suppressing a variety of cancer cell growths, inhibiting telomerase activity, as well as effective in killing with high specificity Taxol-resistant tumor cells at later stage of chemical therapy, making the components a new choice for anti-cancer agent, anti-HIV agent, or synergistic agent.

This invention relates to one method to acquire biology spectrum information and life information and to realize three-dimensional imaging. The invention is characterized by the following: getting multi-photon or single photon triggering of five-dimensional fluorescent microscope imaging information, that is three-dimensional space, one dimensional time and spectrum. This multi-parameter compound measurement and technique, which can satisfy the needs of different layers in study and can realize the flexible made three-dimensional spectrum for measurement.

The invention discloses a method for integrating focusing and detection of cells and a miniaturized system thereof. The miniaturized system comprises a microfluidic chip, a data acquisition card, a miniature computer and a sample injection device, wherein the microfluidic chip is formed by a flow channel layer, a substrate layer and a PCB (Printed Circuit Board) which are aligned and packaged in sequence; the flow channel layer is provided with an asymmetric sinusoidal flow channel, a detection main flow channel, polyelectrolyte gel and a conductive-fluid storage pool; the polyelectrolyte gel, the conductive-fluid storage pool and a silver-silver chloride wire form a detection electrode; the silver-silver chloride wire is connected with the data acquisition card and the miniature computer by a transimpedance amplifier and a differential amplifier so as to form a differential impedance detection circuit of the cells; the miniature computer is used for realizing generation of pseudorandom excitation signals, processing of system response signals and analysis and display of multi-performance parameters of the cells. The method and the miniaturized system disclosed by the invention integrate the functions of focusing and detection of the cells, realizes miniaturization and portability of the system and can be widely used for biological study of blood cells and rare cells.

The present invention relates to nanoparticles the surface of which is modified by deposition of proteins. The invention further relates to a method for producing said nanoparticles and to their use in biological research and in the biomedical field (for example labelling and diagnosis).

A simple and effective stereocontrolled synthesis of salinosporamide A(1)has been developed which follows the pathway outlined in the FIGURE. The process, the first total synthesis of salinosporamide A, is capable of providing the compound in substantial quantities for further biological studies. In addition to the method of Scheme I, the present invention also includes several novel synthetic intermediate compounds, several intermediate steps of the preferred synthetic process; and the uses of these compounds in the preparation of synthetic derivatives of the compound Salinosporamide A. Salinosporamide A is of special interest as a synthetic target because of its protein in vitro cytotoxic activity against many tumor cell lines (IC50 values of 10 nM or less).

The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.

The invention relates to the high-stable increased chemical illuminant base material, which is composed of the solutions A and B. the solution A contains luminal, reinforcing agent phenol and coordinate reinforcing agent; the solution B contains urea peroxide and H2O2. The illuminant signal of the invention is stable and is suitable for the automatic or hand analysis, and the testing results are precise. The 2-8 Deg. C. storing stability can last for two years.

The invention is directed to a model system for structure-activity relationship analysis of peptide or protein molecules involved in important biological processes. Provided by the invention are combinatorial peptide libraries comprising peptides with a novel “tryptophan zipper” scaffold (trpzip) that forms stable β-hairpin structure in solution. Methods of selecting and using such scaffold are provided herein, which are useful for mimicking native protein structures and interactions and designing therapeutic agents. Thus, the invention has profound utility for biological studies and drug development.

The invention relates to synthesis of fluorescent molecules with aggregation-induced emission (AIE) characteristics and application as fluorescent probes for cellular imaging, killing, and antibiotic screening, wherein the cells are bacteria or mammalian cells. The fluorescent molecules generate reactive oxygen species (ROS) upon light irradiation. The invention also relates to a probe comprising fluorescent molecules exhibiting AIE phenomenon, wherein the probe is used for bacterial and biological study. The invention further relates to a method of imaging and quantifying bacterial, a method of killing cells, a method of high throughput antibiotics screening and determining bacteria resistance, a method of photodynamic therapy, and a method of determining critical micelle concentration.

The invention can prepare the microarray of double -stranded DNA(ds DNA) on the surface of solid substrates. By this method, the probes of ds DNA can engomphosised in the nuclear factor kappa B(NF-kappa B) to high-throughpput detect NF-kappa B inside of caryon and screen ds DNA / NF-kappa B molecules interfering dsDNA / NF-kappa B interaction. First, depending on patent technic and others methods of this invention, the invention prepare the microarray of double -stranded DNA(ds DNA) on the surface of solid substrates to allow mounts of dsDNA explorer on the dsDNA micro embattle to link with locus. Second, allow dsDNA micro embattle to across and link with specifically cell nuckoproteides or NF-kappa pure albumen. Third, do qualitative determination or quantiative determination to NF-kappa albumen in special caryon by reaction of NF-kappa B with dsDNA as micro embattle butt.

The invention discloses a multifunctional micro-fluidic chip for cell migration and an invasion assay. The multifunctional micro-fluidic chip comprises a first polydimethylsiloxane (PDMS) film layer, a second PDMS film layer and a glass substrate layer, wherein the first PDMS film layer, the second PDMS film layer and the glass substrate layer are sequentially and irreversibly bonded to form an integral structure; a first micro valve and a second micro valve are arranged on the first PDMS film layer; a first cell culture channel, a second cell culture channel and a third cell culture channel are formed in the second PDMS film layer; the first micro valve is positioned on the upper part of the joint of the first cell culture channel and the second cell culture channel; the second micro valve is positioned on the upper part of the joint of the second cell culture channel and the third cell culture channel. The multifunctional micro-fluidic chip has the advantages of being flexible and easy to operate, reliable to operate, low in manufacturing cost, high in assay success rate, multiple in functions and the like, has high biological study and economic values and is expected to provide a novel research platform for development of medicines for inhibiting tumor cell invasion in the future.

The invention discloses a glass chip for cultivating a single cell array based on microfluidic patterning technology, and a preparation method thereof, and belongs to the field of biological micro electro mechanical systems. The glass chip adopts the structure that a layer of PHEMA hydrogel pattern 2 is arranged on a glass base 4, and divides the glass base 4 into a plurality of rectangular cell growth zones 1 to generate the single cell array. The preparation process is that graphical PHEMA processing is performed on the surface of a glass sheet through a PDMS elastic stamp. The glass chip has the benefits that the stable surface chemical modification is adopted, so that hydrogel dressings capable of resisting cell adhesion is arranged on the surface of the glass in a graphical manner to form the cell graphical surface containing cell adhesion and resisting cell adhesion so as to cultivate the single cell array; the preparation process is simple and easy to operate; when the chip is applied to cell culture, the single cell growth array is obtained, and a new tool for studying basic cell biology is provided.

The invention discloses a method and a device for creating a function curve of an identified material in a system for inspecting an object by utilizing forward scattering radiation. The method comprises the following steps: enabling direct radiation emitted by a radiation source and the forward scattering radiation emitted by a scattering body to interact with known materials with different thicknesses, and detecting to obtain penetration numbers; matching the penetration numbers of two kinds energy to various materials with the same thickness, and calculating a specific value between transparencies under the two kinds of energy; and grouping according to the different materials, and fitting out the variation curve of various materials along with the thickness according to the specific value, wherein the variation curve is used as the function curve of the identified material. The method and the device for creating the function curve of the identified material can be used for performing non-out of box audit on cargoes at customs, harbors and airports, and also can be used for biological studies or medical detection.

The invention provides a terahertz metamaterial device for detecting circulating tumor cells and a preparation method thereof. The device is mainly characterized with a stereo terahertz metamaterial,supplemented by a liquid diversion region. Only a small amount of blood sample is introduced from an inlet hole in a cover plate, and then blood cells of smaller size can be filtered according to structural features. Cancer cells of larger size are fixed at the sensitive positions, namely the clamping positions, of a metamaterial resonant structure. The cancer cells in the sample can be detected by comparing spectral changes before and after the sample is introduced. In addition, the device enables single separation of cancer cells, and can be used for subsequent biological study of individualcancer cells, so as to master the trait changes of each individual cancer cell stimulated by external conditions based on spectral analysis. Compared with the prior art, the device has high sensing sensitivity, and has remarkable effects when being used for fixed-point capture or large cell monitoring.

The invention discloses paecilomyces javanicus strain and the application thereof. The strain adopts paecilomyces javanicus IJ-N2, the strain is preserved in the China Center for Type Culture Collection on tenth of June in 2008 with a CCTCC No of M208085. The strain is wild strain obtained through separating a plutella xylostella body naturally infested by entomogenous fungous from Nonggang Natural Forest Reserve Areas in Guangxi, the paecilomyces javanicus strain is obtained through the wild strain being back inoculated to the plutella xylostella body for rejuvenation, and depurative strain is obtained through the monospore separating operation being performed to the strain. The paecilomyces javanicus strain has strong infestation and insecticidal effect to aleyrodids, solenopsis invicta buren, spodoptera litura, aphid and plutella xylostella according to long-term infection biology study and indoor bioassay.

The invention discloses a micro-flow control chip for cell tissue culture and real-time monitoring and a use method thereof. The chip comprises a glass substrate layer and a PDMS micro-flow channel layer positioned on the glass substrate layer; the glass substrate layer comprises a glass substrate and a plurality of pairs of microelectrodes arranged on the glass substrate layer; the PDMS micro-flow channel layer comprises a plurality of independent micro-fluidic channels; the microelectrodes on the glass substrate are corresponding to the micro-flow channels on the PDMS micro-flow channel layer one by one; the microelectrodes are electrically connected with an external circuit. The use method comprises the steps of cell capture, cell tissue culture, electrical impedance spectroscopy detection and tissue release. The chip disclosed by the invention is processed by a transparent substrate, microscopic imaging can be taken as a supplementary means of the electrical impedance spectroscopydetection, and the dynamic growth and the physiological behavior of cell tissues and other biological processes are subjected to observation and in-situ analysis. The micro-flow control chip can be used in the fields of biological research, drug screening and the like of cells or tissues.

The invention, which belongs to the field of biochemistry, relates to a tumor-targeting functionalized quantum dot and a preparation method thereof. The functionalized quantum dot consists of folic acid, cyclodextrin and quantum dot, and part of folic acid molecules are contained in the hydrophobic cavity of the cyclodextrin to form folic acid-cyclodextrin inclusion compound, which is then combined with the surface of the quantum dot. In the process of preparing the functionalized quantum dot, the folic acid is first combined with the Gamma-cyclodextrin to form the inclusion compound, and the Gamma-cyclodextrin-folic acid inclusion compound is then modified on the surface of the quantum dot, so that the dual-function nano material with excellent fluorescent characteristic and tumor-targeting property is produced. Since the Gamma-cyclodextrin is used to include the folic acid, the water-solubility and dispersibility of the folic acid are improved. The folic acid and the quantum dot are connected together by an effective method, and the produced fluorescent probe can be used for biological research on tumor cells. The preparation method is simple, the cost is low, and the screening of a variety of tumor cells and an in-vivo experiment on mice indicate that the material is characterized by low toxicity, high selectivity and the like.

The present invention relates to nanometer magnetic fluorescent microsphere and its preparation process and application. The nanometer magnetic fluorescent microsphere is one core-shell including core of Eu3+Y-Fe2O3 compound, shell of SiO2 coated fluorescent material and surface modifying organic functional group. It is prepared through reverse micro emulsion process to synthesize nanometer particle, and the subsequent surface chemical modification to become magnetic nanometer particle capable of combining with probe. The nanometer magnetic fluorescent microsphere is used as biological research tool with integrated fluorescent indication and magnetic separation, and may have important application in biomicromolecule detection, medical diagnosis and other fields.

The invention discloses an aschersonia aleyrodis strain and the application of the strain. The aschersonia aleyrodis strain is a wild strain separated from bemisia tabaci body in Guangzhou area, which is naturally infected by worm living fungus. The wild strain is multi-planted on the bemisia tabaci body for the rejuvenation, so that the aschersonia aleyrodis strain is obtained; the purified strain is obtained through single spore isolation. The paecilomyces fumosoroseus strain is AA01-N8; the preservation number of the strain is CCTCC No: M207087; the strain is stored in China Center for Type Culture Collection on 27 June, 2007. Through a long term of infectious biology research and the indoor biology testing, the aschersonia aleyrodis has strong infectious and killing effects for a plurality of sucking mouth parts insects, such as the white fly and the cotton worm etc.

The invention discloses a nonglutinous rice callus culture medium and a genetic transformation method mediated by agrobacterium tumfaciens. The culture medium contains major elements, minor elements, organic elements and hormone in different culture stages which are required for the growth of nonglutinous rice calluses. The genetic transformation method of nonglutinous rice based on the culture medium comprises the steps of induction, subculture, pre-culture, co-culture, sieving, aftersieving, differentiation, rooting of the calluses and the like. The method is widely suitable for nonglutinous rice genotypes, all genetic transformation of the tested 35 nonglutinous rice genotypes are successful, and the transformation rate is between 20% and 50%. The genetic transformation method solves the technical problem of low genotype reliance and transformation frequency existing in the nonglutinous rice callus culture and mediation of agrobacterium tumfaciens for a long time, and is widely applied to the research in the molecular biology, the functional genomics of the nonglutinous rice and the genetic improvement of varieties. The planting area of the nonglutinous rice accounts for more than 80% of the total production area of the nonglutinous rice, and the genetic transformation method has an important function on the research of the basic biology, the improvement of new varieties and the safety guarantee of future grain.

The present invention relates to a method for detecting target nucleic acids by simultaneous isothermal amplification of the target nucleic acids and a signal probe 5 using an external primer set, a DNA-RNA-DNA hybrid primer set and a DNA-RNA-DNA hybrid signal probe. The method according to the present invention can be used to amplify target nucleic acids in a sample, rapid and exact manner without the risk of contamination compared to the conventional methods such as PCR, and it can simultaneously amplify target nucleic acid and a signal probe, so that it can 0 be applied to various genome projects, detection and identification of a pathogen, detection of gene modification producing a predetermined phenotype, detection of hereditary diseases or determination of sensibility to diseases, and estimation of gene expression. Thus, it is useful for molecular biological studies and disease diagnosis.

The invention discloses a method and a device for detecting an object by using forward scattered radiation. The method comprises the following steps: detecting a first penetration value after the interaction between first radiation generated by a radiation source and a detected object; making second radiation generated by the radiation source interact with a scatterer so as to generate the forward scattered radiation having a preset angle with the second radiation; detecting a second penetration value after the interaction between the forward scattered radiation and the detected object; and obtaining material attribute information of the detected object by using the detected first penetration value and the second penetration value. The method and the device can be applied to detection of goods without opening boxes at customs houses, harbors and airports, and can also be applied to biological studies or medical detections.