72 results about "Early disease" patented technology

New and improved applications of Raman Scattering are disclosed. These applications may be implemented with or without using an enhanced nano-structured surface that is trademarked as the RamanNanoChip™ disclosed in a pending patent. As a RamanNanoChip™ provides much higher sensitivity in SERS compared with conventional enhance surface, broader scopes of applications are now enabled and can be practically implemented as now disclosed in this application. Furthermore, a wide range of applications is achievable as new and improved Raman sensing applications. By applying the analysis of Raman scattering spectrum, applications can be carried out to identify unknown chemical compositions to perform the tasks of homeland security; food, drug and drinking materials safety; early disease diagnosis; environmental monitoring; industrial process monitoring, precious metal and gem authentications, etc.

Devices, systems and methods are provided for the real-time detection of disease through constant or periodic monitoring of biomolecules in an individual without laboratory sampling. Biomolecule detector devices may be worn in contact with skin, or be implanted in fluid communication with a biological fluid to provide information and identity of disease-related circulating biomolecules in an individual.

The invention discloses a water-based road asphalt rehabilitating agent and a preparation method thereof. The formula of the water-based road asphalt rehabilitating agent comprises the following components in percentage by weight: 20 to 30 percent of asphalt, 10 to 20 percent of water, 5 to 10 percent of solvent, 10 to 20 percent of modifier, 0.5 to 2 percent of emulsifier, 0.5 to 1 percent of adhesion promoter, 0.5 to 2 percent of antisettling agent and 25 to 40 percent of filler. The preparation method comprises the following steps of: mixing the water and the emulsifier at the temperature of between 55 and 60 DEG C uniformly; adding the asphalt and the modifier which are diluted by the solvent sequentially; emulsifying the materials in asphalt emulsifying equipment; adding the adhesion promoter, the antisettling agent and the filler sequentially; and continuously stirring for 15 to 30 minutes under the conditions of normal temperature, normal pressure and high speed to obtain the finished product. The water-based road asphalt rehabilitating agent has high permeability, cohesive property and durability, is used for beautifying road surface and repairing early diseases such as ageing of the asphalt road surface, pitting surface, water seepage and the like, prolongs the service life of the road by 2 to 3 years, and has remarkable economic and social benefits.

The invention discloses a magnetic and luminous bifunctional hydroxyapatite nanoparticle in a core-shell structure and belongs to the technical field of nano functional material preparation. According to the method, pure hydroxyapatite continues to grow on the surface of a magnetic hydroxyapatite nanoparticle by controlling a coprecipitation condition to realize hydroxyapatite thin layer coating of the magnetic particle, so that dissolving of magnetic ions (such as Fe<3+>) quenching luminescence of a rare earth ion is inhibited. Rare earth doped hydroxyapatite continues to grow on the surface of the magnetic hydroxyapatite nanoparticle with a hydroxyapatite coating layer, so that the magnetic and luminous hydroxyapatite nanoparticle in the core-shell structure is obtained. The core-shell type magnetic and luminous hydroxyapatite nanoparticle has good magnetic property, luminescence property and biocompatibility, further has target positioning and mark tracking functions, and has good application prospects in the field of biomedicine such as substance detection inside and outside a cell, nucleic acid hybridization and sequencing, early disease diagnosis, transport of gene drugs, and magnetic heat treatment.

The present invention relates to the analysis and monitoring of ocular fluids for determining the physiological state of an organism, to monitor drug efficacy and dynamics, for early disease detection, as well as to certain molecular markers and fingerprints of identified molecules or molecule fragments in such analysis.

The present invention relates to markers and diagnostic reagents for the diagnosis or prognosis of lung cancer. The present invention discloses for the first time that DDX56 is significantly increasedin patients with early disease progression (= 12 months) after treatment of two major subtypes of small cell lung cancer (lung squamous cell carcinoma, lung adenocarcinoma), and that the increased expression of DDX56 significantly affects the overall survival of the patients. Therefore, DDX56 can be used as a diagnostic marker for early recurrence of lung squamous cell carcinoma or lung adenocarcinoma.

The invention discloses a maintenance construction method of an asphalt road surface solid permeability wearing layer. The construction method includes the following steps that the spreading amount ofa solid permeability agent is surveyed; a road surface is cleaned; the road surface is pre-processed; the solid permeability agent is sprayed; a wearing layer is spread; manual trimming is carried out; preliminary maintenance is carried out. According to the maintenance construction method, the solid permeability agent is demulsified immediately after being atomized or smeared on the original road surface, heavy cycloparaffin distilled oil rapidly infiltrates to restore old asphalt performance, early diseases of the original asphalt road surface are repaired, and meanwhile a matrix asphalt and tackifier mixture is left on the road surface to form a binding layer. A solid permeability layer is formed by the repaired original asphalt road surface and the binding layer. The maintenance construction method is high in construction speed and efficiency.

Screening apparently healthy individuals using sensitive techniques for identifying early vascular and cardiac disease provides the opportunity for effective intervention to prevent or delay the occurrence of cardiovascular events which represent the most common cause of morbidity and mortality in our society. Preliminary studies in the have revealed an incidence of over 50% of early disease in need of therapy. The apparent ineffectiveness of the health care system to uncover this early disease emphasizes the need for a more aggressive community approach to screen for early disease and to initiate therapy that should reduce health care costs and improve quality and duration of life.

The invention discloses a multilayer information monitoring early warning method of early diseases of crops. The objective is to provide a lossless monitoring method of early diseases of crops. The method comprises steps of shooting the whole crop to obtain an object infrared image; calculating the average temperature of a monitoring object pixel point, the average temperature of a low-temperature pixel point and the average temperature of a high-temperature pixel point, and calculating the temperature difference between the average temperature of the low-temperature pixel point and the average temperature of the high-temperature pixel point; comparing the temperature difference with a monitoring threshold value and taking the temperature difference as the basis for determining whether the crops are infected with diseases; using a visible light-near infrared high spectral imaging device to monitor the crops suffering the diseases to obtain characteristic images; using a microscopy imaging device to monitor disease speckles in the characteristic images,= to obtain microscopy images; and according to a microscopy image gray scale histogram, estimating the early disease degree of the crops by combining the characteristic images. According to the invention, multilayer information of temperatures, pigments and textures of the crops is monitored; and disease detection and precise analysis in the incubation period and at the disease early stage are achieved.

The invention discloses a kit for quantitatively detecting GFAP concentration in human serum by polystyrene microsphere, comprising a 96-hole filter plate, a standard product, a sample diluent, a polystyrene microsphere coupled with an anti-GFAP antibody on the surface, a microsphere storage solution, an enzyme compound, a substrate A, a substrate B, a 20*cleaning solution, an adhesive sticker strip and an operating manual. The kit provided by the invention changes the solid phase carrier made of traditional microporous plate into the polystyrene microsphere, generates the antigen antibody reaction in the liquid phase to keep a good bioactivity and a correct spatial configuration, and eliminates the effect on the antigen antibody reaction by the surface tension and the steric hindrance ofthe traditional reaction mode, and effectively shortens the reaction time and the rinsing times, besides, the invention has the characteristics of simple operation, high sensitivity, good specificityand low cost and has important clinical significance for the diagnosis of the acute cerebral hemorrhage early disease degree.

The invention provides LAMP (Loop-mediated Isothermal Amplification) primers for rapidly assaying tea tree anthracnose pathogens and an assay method. Aimed at the beta-tubulin(TUB2) gene sequence of tea tree anthracnose pathogens, the invention designs and screens out a set of specific assay primer group, and the primer group consists of four specific primers F3, B3, FIP and BIP. The primers screened out by the invention have high sensitivity and strong specificity, the established assay method ha the advantages of high accuracy, strong specificity, easiness in operation, short assay time, simple instruments and equipment and the like, and is applicable to the high-sensitivity rapid assay, identification and early disease diagnosis of tea tree anthracnose pathogens in diseased tissues andpathogen-carrying plants, and for the early monitoring of tea tree anthracnose in agricultural production, the invention is of great significance for the determination of optimal prevention and control time.

The invention discloses an OCT (Optical Coherence Tomography) image analyzing method based on speckle contrast, comprising the following steps of: firstly, acquiring a single-width OCT image of an object to be imaged by using an OCT device, and selecting a processing window; calculating the contrast of pixel intensity in the processing window; moving the processing window and calculating the contrast of the pixel intensity in the processing window until traversing the image of the whole OCT; and according to the values of the contrasts, performing false color dying on the image. In the early disease diagnosis, the changes such as a micron dimension of intracellular fine structures need to be respectively distinguished, and the change of the micron-dimension cell level can be extracted by adopting the OCT image analyzing method provided by the invention.

The invention relates to a highway asphalt material technique, and discloses a gangue asphalt modifier, a modified asphalt mixture and preparation methods of the gangue asphalt modifier and the modified asphalt mixture. The gangue asphalt modifier comprises the following components by weight percentage: 70-90% of activated gangue powder, 10-20% of quick lime, and 10-20% of natural brucite fiber. The modified asphalt mixture is characterized in that the modified asphalt mixture comprises the following components in parts by weight: 5-8 parts of gangue asphalt modifier, 4-6 parts of asphalt and 88-92 parts of aggregate. The gangue asphalt modifier can obviously improve high-temperature stability, low-temperature cracking resistance and water stability of the asphalt mixture, can effectively prevent and control early diseases of a pavement, and can prolong the service life of an asphalt pavement; in addition, the gangue asphalt modifier is prepared by taking gangue as a main raw material, full use of industrial residues is facilitated, and the highway construction cost is lowered, so that higher economic and social benefits can be obtained.

The invention discloses a single photon emission computed tomography developer and a preparation method thereof. The method is characterized by respectively synthetising two peptides by the solid-phase synthesis to obtain corresponding initial products and final products, synthetising a single compound with a first kind feature structure (I) or a second kind feature structure (II) and forming the developer according to a needed dosage ratio. The imaging developer is micro-molecule, good in hydrophilicity and easy to prepare, and precursor micro-molecule of the developer has peptides specially recognized by specific enzyme, so that imaging areas can be targeted actively, and controlled self-assembly of radioactive nano-structures in living cells is realized to study activity of specific enzymes in tumor cells. Compared with conventional micro-nano systems used in early disease diagnosis, by the preparation method, micro-nano materials synthetised in vitro are not needed to be injected into living bodies for tumor diagnosis, problems of low intake and difficulty in targeting of conventional nano-materials are solved.

The invention discloses a kit for early screening of the lung cancer based on peripheral blood. The kit comprises a connector, a PCR primer and a capture probe. The connector comprises a Tag sequence,a Poly N sequence, a first hairpin sequence, a second hairpin sequence, a first index sequence, a first common primer combination sequence, a second common primer combination sequence and dUTP, and the first index sequence is used for distinguishing different peripheral blood samples; the PCR primer is a primer designed for 358 genes which are related to the pulmonary nodule and indirectly influence proliferation and differentiation of cells, and the PCR primer is used for being combined with the connector; the capture probe is a probe designed for the 358 genes which are related to the pulmonary nodule and indirectly influence proliferation and differentiation of the cells, and the capture probe is used for capturing a cfDNA area of plasma in the peripheral blood samples. The provided kit can be applied to early disease screening of the lung cancer, and the pulmonary nodule is diagnosed in a noninvasive and high-sensitivity mode.

The invention discloses a primer group for detecting field pectobacterium carotovorum subsp and a kit and method for extracting and detecting DNA of the pectobacterium carotovorum subsp. The kit combines a sample nucleic acid rapid extracting technology and a loop-mediated isothermal amplification technology to establish a bacterial soft rot pathogenic bacterium product based on a soft rot pathogenic bacterium specific target single-base difference phenomenon. The detection method has the advantages that soft rot pathogenic bacteria, plant tissue and soil samples can be rapidly detected separately, nucleic acid extraction can be completed within 30 seconds, detection only needs 30-40 minutes, detection is rapid and accurate, no expensive instrument is needed, the cost is low, and the detection method can be directly used for real-time and on-site detection in the field, which facilitates dynamic monitoring of the soft rot pathogenic bacteria in soil, facilitates the development of popular prediction and early warning work of the soft rot, and facilitates early disease prevention and treatment.

Musculoskeletal fibroproliferative disorders, such as Dupuytren's disease may be treated by administering locally a TNF-α antagonist. TNF-α antagonists find particular utility in inhibiting the progression of early disease state Dupuytren's disease and other musculoskeletal fibroproliferative disorders and, in combination with extracellular matrix degradation agents (such ascollagenase or matrix metalloproteinase I), treating advanced disease state Dupuytren's disease and, in particular inhibiting recurrence.

The invention relates to Blackberrylily rhizome antiviral injection, preparation and venous medicine feeding blackberrylily rhizome antiviral injection thereof. The original medicine comprises 1-1000 portion of belamcanda rhizome by weight, 1-1000 portion of honeysuckle by weight, 1-1000 portion of Eupatorium by weight, 1-1000 portion of capilary artemisia by weight, 1-1000 portion of radix bupleuri by weight, 1-1000 portion of dandelion by weight, 1-1000 portion of isatis root by weight, 1-1000 portion of dyers woad leaf by weight. The medicine is obtained by following steps: distilling by vapor, boiling, adding adding clarifier, alcohol-settling, water-settling, centrifugation, hyperfiltration to get active component of the Blackberrylily rhizome antiviral injection, and then subpackaging, disinfecting to get finished preparation, in which the chlorogenic acid (C16H18O9) is not less than 0.5 mg / ml. The injection has the function of antivirus and killing bacteria and relieving inflammation and treating epidemic hemorrhagic fever early disease. The invention avoids the medicine liquid thickness, deposition and color changing, stabilizes the preparation, prolongs the product effective date. The injection can be applied through vein so as to prevent the adverse effects such as pain in the administration location, blood stasis and even muscle necrosis.

The invention relates to a method and a device for judging different disease stresses of cucumbers based on a chlorophyll fluorescence imaging technology. The diagnosis method comprises the followingsteps of collecting cucumber canopy fluorescence images under different light intensities by changing voltage; extracting fluorescence parameter information of leaf areas; carrying out image segmentation and feature extraction on the leaf scab areas; analyzing the difference of fluorescence parameters under different disease stresses; and establishing a cucumber disease stress judgment model by adopting multiple classifiers. According to the cucumber disease stress diagnosis method, fluorescence parameters of a whole plant can be provided to locate a disease spot area, different diseases can be diagnosed according to the difference of the fluorescence parameters, and early diseases of the plant can be recognized.

The invention discloses a PSR (Polymerase Spiral Reaction) detection primer for escherichia coli O157:H7, a kit and a detection method thereof. The primer comprises detection primers Ft and Bt, and acceleration primers IF and IB, wherein the nucleotide sequence of the detection primer Ft is shown as SEQ ID NO. 1; the nucleotide sequence of the primer Bt is shown as SEQ ID NO. 2; the nucleotide sequence of the acceleration primer IF is shown as SEQ ID NO. 3; the nucleotide sequence of the acceleration primer IB is shown as SEQ ID NO. 4. The invention further provides a PSR detection kit for theescherichia coli O157:H7. By adopting the PSR detection primer, the kit and the detection method thereof, a detection result can be obtained in about 40 minutes, and meanwhile the detection reliability, specificity and sensitivity can be ensured. The PSR detection primer, the kit and the detection method thereof are particularly suitable for middle-small units and field detection, and are of veryimportant significance to the increase of diagnosis rate of significant group diseases, and early disease diagnosis.

The invention discloses a method for monitoring a crop disease and comprehensively evaluating the degree of the disease by virtue of an infrared image, and belongs to an optical nondestructive early crop disease monitoring method. The method comprises the following steps: shooting the whole crop or shooting all crops in a certain planting region to obtain an infrared image of a monitoring object; calculating the average temperature of all pixels of the monitoring object, the low-temperature area sum of low-temperature pixels, the high-temperature area sum of high-temperature pixels and the total temperature area of all the pixels according to the infrared image respectively, and calculating the ratio of the low-temperature area sum to the total temperature area; taking the ratio as a comprehensive valuation parameter for the degree of the crop disease. According to the method, an early disease imperceptible for eyes can be nondestructively and accurately identified on line, and the detection and analysis time and cost can be reduced; the method can also be used for comprehensive evaluation for nondestructive monitoring of the health condition of the crop.

Provided herein are methods for the diagnosis and treatment of human melanoma and prediction of early disease genesis to metastasis by assessing CpG island methylation or expression level of epigenetically regulated differentially expressed coding or non-coding genes. Methods of treatment of human melanoma by modifying or regulating the same pathways are also provided.

The invention belongs to the technical field of plant root-knot nematode inoculation methods, and particularly provides a plant root-knot nematode potting three-layer sick soil inoculation method. Themethod comprises the steps of 1, disease-free soil collection and treatment; 2, sick soil collection and treatment; 3, sick soil expanding propagation cultivation; 4, expanded propagation sick soil pretreatment; and 5, three-layer sick soil inoculation. The plant root-knot nematode potting three-layer sick soil inoculation method adopts a three-layer sick soil inoculation technology that disease-free soil is inoculated on upper and lower layers, and nematode sick soil is inoculated on a middle layer. Compared with an existing root-knot nematode egg and larvae inoculation technology, the technology approaches natural inoculation, the inoculation survival rate is high, a nematode selecting link is omitted, and the workload is greatly reduced. Compared with an existing root-knot nematode single sick soil inoculation technology, the technology has the advantages that the usage amount of the sick soil is reduced by 1 / 2-2 / 3, and the workload and cost of the collection, transportation, expanding propagation cultivation and the like of the sick soil are greatly reduced; and newly transplanted seedlings can be protected from being infected by nematodes, not only can the success of the inoculation be ensured, but also too early disease death of the seedlings can be avoided.

A system for predicting diseases may accumulate information about the volatile, semi-volatile, and non-volatile organic compounds in breath / saliva. Such information may be analyzed over time to identify early disease indications, using non-invasive data collection via breath and alert patients directly for follow-up with a health professional.