137 results about "Stripe rust" patented technology

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat magnesium transporter TaNIPA8-6B and application of a coding gene thereof to regulation and control of plant stripe rust resistance. Functional analysis is carried out on the wheat magnesium transporter TaNIPA8-6B by means of gene editing and overexpression, and it is found that the wheat magnesium transporter TaNIPA8-6B induces expression in both affinity and non-affinity system reactions of wheat and puccinia striiformis, so that a mutant plant of the gene editing and overexpression TaNIPA8-6B gene is created; according to the TaNIPA8-6B gene, the gene knockout mutant of the TaNIPA8-6B gene is identified to show susceptibility to stripe rust non-compatible races CYR23, the over-expression mutant plant shows disease resistance to stripe rust compatible races CYR32, the fact that the TaNIPA8-6B gene plays a positive regulation role in the disease resistance of wheat to stripe rust is clear, and a high-quality germplasm resource is provided for stripe rust resistance of wheat.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention provides application of wheat cysteine receptor kinase TaCRK25-1B and a coding gene thereof in regulation and control of stripe rust resistance of plants, and belongs to the technical field of plant genetic engineering. The TaCRK25-1B provided by the invention is subjected to induced expression of stripe rust infection in wheat, a VIGS technology instantaneous silencing experiment shows that the resistance of the wheat to stripe rust CYR31 is enhanced by silencing the TaCRK25-1B, the TaCRK25-1B is over-expressed in the wheat by a genetic transformation technology, the resistance of the wheat to stripe rust is weakened, and an RNAi interference plant infection pathogen experiment of the TaCRK25-1B created by an RNAi technology shows that the TaCRK25-1B has the advantages that the TaCRK25-1B can be used for expressing the stripe rust CYR31 in the wheat; the TACRK25-1B plays a negative regulation role in wheat stripe rust resistance. Therefore, the invention provides a new means for wheat stripe rust resistance and resistant wheat breeding.

The invention belongs to the field of biological agriculture, and particularly relates to application of a wheat stripe rust effect protein gene HASP 38 in improvement of stripe rust resistance of wheat. The amino acid sequence of the wheat stripe rust effect protein HASP 38 provided by the invention is as shown in SEQ ID NO: 1, the nucleotide sequence of the wheat stripe rust effect protein gene HASP 38 is as shown in SEQ ID NO: 2, and an RNA interference vector of the gene is provided. According to the invention, an RNA (Ribonucleic Acid) interference vector of the gene is transformed into a Fielder wild type material, so that the HASP 38 gene silencing wheat with resistance to stripe rust CYR32 is obtained. According to the invention, the wheat stripe rust effect protein gene HASP 38 is determined to play a negative regulation role in wheat stripe rust resistance, and a stripe rust resistant wheat material is created by using the negative regulation factor, so that a new gene and germplasm resource are provided for wheat stripe rust resistant breeding.

The invention discloses a wheat stripe rust molecular marker and application thereof in stripe rust resistance identification. The wheat stripe rust molecular marker disclosed by the invention is nucleotides corresponding to 29th to 32th sites of SEQ ID No.2 in a wheat genome, and the nucleotides are 29th to 32th sites of SEQ ID No.2 or 29th to 32th sites of SEQ ID No.2 deleted. Experiments prove that wheat materials with stripe rust resistance can be quickly screened out by applying the molecular marker disclosed by the invention, so that the breeding pace of new wheat varieties is accelerated. The method has important theoretical significance and economic value.

The invention belongs to the technical field of gene engineering, and discloses application of a transcription factor TaNAC67 in regulation and control of wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown as SEQ ID NO: 1, and the transcription factor can induce cell necrosis and exert a positive regulation function in the interaction process of wheat and puccinia striiformis. The coding gene TaNAC67 of the transcription factor is a disease-resistant related gene, is infected by stripe rust in non-affinity interaction of plants and stripe rust and is strongly induced to express, and the nucleotide sequence is shown as SEQ ID NO: 2. On the basis of the characteristics, a transgenic plant over-expressing the TaNAC67 gene is further obtained, and it is verified that the transgenic plant shows resistance to stripe rust. The invention provides gene resources and technical support for subsequent genetic improvement of stripe rust resistant wheat materials.

The invention discloses a KASP molecular marker related to wheat stripe rust resistance characters and application of the KASP molecular marker, and belongs to the technical field of molecular markers and plant genetic breeding. SNP sites QYr.hzau4B of the KASP molecular marker are located at the 452, 424 and 208 basic groups of a wheat reference genome 4B chromosome, C / T mutation exists at the sites, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO. 1. The KASP molecular marker can be used for identifying stripe rust resistant and susceptible varieties or strains of wheat, and a valuable molecular tool is provided for screening stripe rust resistant germplasm resources of wheat, so that the breeding efficiency of improved varieties is improved, and the breeding process is accelerated.

Compositions and methods for enhancing the resistance of wheat and barley plants to wheat stripe rust caused by Puccinia striiformis f. sp. tritici are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of wheat and barley plants to wheat stripe rust comprise introducing a nucleic acid molecule encoding an R gene product into a wheat or barley plant cell. Additionally provided are methods for using the wheat and barley plants in agriculture to limit wheat stripe rust.

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.

The application discloses a kind of molecular markers of wheat stripe rust SNP homozygous site based on whole genome sequence and application thereof.The application is analyzed to 28 strains of global wheat stripe rust genome sequence, compared with reference genome, and more than a million SNP sites are mined out.Sequence depth screening, linkage disequilibrium analysis and heterozygosity detection, identify 1076 SNP homozygous sites.Further based on the frequency of secondary allele, screening of deletion rate and adjacent simple repeat sequence, finally determine 37 core SNP markers.Through primer design to these SNP sites, and add fluorescent linker, develop KASP-SNP molecular marker, which can be used for accurate genotyping identification of wheat stripe rust population.The molecular marker developed based on global wheat stripe rust whole genome is suitable for wheat stripe rust population research in all regions of the world.Based on the development of molecular marker of homozygous site, polymorphism difference caused by heterozygous site can be effectively excluded, to ensure the accuracy of detection site.The set of KASP-SNP molecular marker has high polymorphism, good repeatability and high detection efficiency, and can be widely applied to genetic research of wheat stripe rust population.

The application provides application of a wheat ELP2 gene in regulating resistance of wheat to stripe rust, and belongs to the technical field of genetic engineering. The application provides application of the wheat ELP2 gene in regulating resistance of wheat to stripe rust, also provides a silencing fragment of the wheat ELP2 gene obtained by an amplification method, and gene silencing of the wheat ELP2 gene is carried out by using the silencing fragment, and in the examples, overexpression plants of the ELP2 gene are constructed, it is found that the overexpression weakens the resistance of wheat to non-compatible races of Puccinia striiformis f. sp. tritici, the interaction relationship between ELP2 and the PAZ motif of DCL1 is verified by using yeast double hybridization, LCI and Pull-down, meanwhile, the resistance of wheat to compatible races is enhanced and the infection of Puccinia striiformis f. sp. tritici is inhibited after silencing DCL1 in wheat by VIGS, therefore, the ELP2 gene plays a disease function in the interaction between wheat and Puccinia striiformis f. sp. tritici.

The invention provides a GMRM (Genome-based Mutant RNA Mapping) method taking a genome as a reference, in particular, the invention provides a method for cloning candidate functional genes of wheat stripe rust resistant plants and an analysis process, and the GMRM method disclosed by the invention can be used for simultaneously cloning two genes with complementary disease-resistant functions, namely Yr6NLR1 and Yr6NLR2. In an example of Yr6, the two disease-resistant genes are closely linked genetically, but the cloning process of the genes does not involve any genetic recombination. Therefore, theoretically, the method provided by the invention can be used for simultaneously cloning a plurality of functional complementary genes (namely genes participating in the same pathway) on the premise that the target gene can generate effective mutation through EMS mutagenesis.

The invention discloses a wheat stripe rust adult plant resistance gene Yr54, a specific functional molecular marker and application of the wheat stripe rust adult plant resistance gene Yr54 in wheat stripe rust resistance breeding, and belongs to the technical field of biology. A wheat stripe rust adult-plant resistance gene TaNLR2DL transgenic line is subjected to adult-plant-stage stripe rust resistance identification, and a result shows that compared with a non-TaNLR2DL gene line, the stripe rust resistance of the TaNLR2DL transgenic line is obviously improved, while a TaNLR2DL gene knockout line is contrary to the TaNLR2DL gene knockout line, so that the TaNLR2DL is a potential stripe rust resistance gene, and the TaNLR2DL gene knockout line can be used for identifying the stripe rust resistance in the adult-plant stage. The method can be used for genetic improvement of wheat stripe rust resistance. The invention further develops a functional specific molecular marker of TaNLR2DL, and the marker can quickly screen wheat germplasm containing TaNLR2DL, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

This invention relates to the field of crop breeding technology, specifically to a highly efficient breeding method for early-maturing highland barley. The method includes the following steps: parent selection and matching, planting substrate preparation, application of growth regulators, planting management, multi-generation screening, and variety identification. This invention, by screening parents with early-maturing and disease-resistant characteristics, optimizing the planting substrate (adding microbial agents), precisely applying growth regulators, and dynamically controlling the planting environment and nutrient solution, combined with early maturity, disease resistance, and variety identification, efficiently breeds highland barley with a short growth period and strong resistance to stripe rust and powdery mildew. It optimizes plant type and yield structure, improves grain quality, achieves increased yield and genetic stability, and provides technical support for highland barley variety improvement and high-yield, high-quality cultivation.

Provided are a strain of Bacillus velezensis EA19 and the use thereof. The strain is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO:M2023602. The strain has a relatively good treatment effect and prevention effect on stripe rust of Triticuma aestivum, and shows a comparable control efficacy to triadimefon. Moreover, the strain has an effect of reducing dosage while enhancing efficacy when used in combination with mefentrifluconazole. Therefore, the strain has important application values in the control of agricultural pests and diseases, provides a new way for green control of stripe rust of Triticuma aestivum, and has broad application prospects.

The invention discloses a molecular marker for detecting stripe rust and brown leaf rust resistant one-cause multiple-effect sites of wheat and a use method, and belongs to the technical field of nucleotide polymorphism and molecular markers. The technical problem to be solved by the invention is how to quickly and accurately screen or assist in screening wheat with stripe rust or / and leaf rust resistance or / and breed or assist in breeding wheat single plants with stripe rust or / and leaf rust resistance. The invention provides a composition for detecting single nucleotide polymorphism or genotype of a specific SNP site in a wheat genome, the SNP site can be the 47th nucleotide of a DNA fragment as shown in SEQ ID NO: 1 in the wheat genome, and the type of the nucleotide can be A or T. The primer composition and the molecular marker provided by the invention assist in wheat breeding for resisting stripe rust and leaf rust in wheat disease resistance breeding, greatly shorten the breeding period and play an important role in wheat breeding.

The invention belongs to the technical field of molecular biology, and relates to a KASP molecular marker for wheat stripe rust resistance gene detection and application. The KASP molecular marker XK6B-5 disclosed by the invention is obtained by utilizing a specific primer group as shown in SEQ ID NO.2-4 for amplification; the KASP molecular marker XK6B-5 is closely linked with a wheat stripe rust resistance gene YrZT94.1, and has a co-separation characteristic, and the polymorphism of the XK6B-5 is G / T. The KASP molecular marker provided by the invention can accurately track the stripe rust resistance gene YrZT94.1 of wheat, performs molecular marker-assisted selection, and improves the breeding efficiency of wheat disease resistance. The method can be applied to screening of the stripe rust resistant gene YrZT94.1 in wheat germplasm resources in a simple, rapid and high-throughput manner, so that the method is applied to molecular design breeding, and the wheat breeding process is greatly accelerated.

The invention belongs to the technical field of molecular biology, and relates to a KASP molecular marker for detecting a wheat stripe rust resistance gene YrZT94.2 and application of the KASP molecular marker. The KASP molecular marker XK7B-32 disclosed by the invention is obtained by utilizing a specific primer group as shown in SEQ ID NO.2-4 for amplification; the KASP molecular marker XK7B-32 is closely linked with a wheat stripe rust resistance gene YrZT94.2, and has a co-separation characteristic, and the polymorphism of the XK7B-32 is C / G. The KASP molecular marker provided by the invention can accurately track the stripe rust resistance gene YrZT94.2 of wheat, performs molecular marker-assisted selection, and improves the breeding efficiency of wheat disease resistance. The method can be simply and quickly applied to screening of the stripe rust resistant gene YrZT94.2 in wheat germplasm resources with high throughput, so that the method is applied to molecular design breeding, and the wheat breeding process is greatly accelerated.

This invention belongs to the field of genetic engineering technology and relates to the application of wheat receptor kinase TaSPR12 in improving wheat disease resistance. This invention utilizes reverse genetics methods to analyze and screen wheat receptor kinase genes. TaSPR12 This gene is induced to express by the stripe rust pathogen. The wheat receptor kinase gene was knocked out using CRISPR-Cas9 gene editing technology. TaSPR12 The created loss-of-function mutants exhibited enhanced resistance to the main prevalent races of wheat stripe rust. This invention identified the wheat receptor kinase gene. TaSPR12 It plays a negative regulatory role in wheat resistance to stripe rust and can be used for genetic improvement of wheat resistance to rust.

The invention belongs to the technical field of biology, and particularly relates to a plant disease resistance related protein TaCNGC10 as well as a coding gene and application thereof. The invention provides a plant disease resistance related protein TaCNGC10. The amino acid sequence of the plant disease resistance related protein TaCNGC10 is as shown in SEQ ID NO: 2. According to the invention, function analysis is carried out on related proteins and genes, so that a disease-resistant mechanism of the protein and the genes in interaction of plants and stripe rust is disclosed, and a solution is provided for cultivation of stripe rust-resistant materials.

This invention belongs to the field of genetic engineering technology and discloses... TaRNS4 Application of genes in wheat resistance to stripe rust. TaRNS4 The nucleotide sequence of the gene is shown in SEQ.ID.NO:1. This gene is involved in wheat resistance to stripe rust and plays a positive regulatory role in wheat resistance to stripe rust. Overexpression of the gene... TaRNS4 Genes enhance wheat's resistance to stripe rust. Overexpression TaRNS4 The gene can activate the wheat MAPK signaling pathway, increase the expression of disease resistance-related genes in wheat, thereby improving wheat's resistance to stripe rust. Overexpression TaRNS4 The gene can also reduce yield loss in wheat caused by stripe rust. This invention provides a germplasm resource for the green control of wheat stripe rust and offers new ideas for breeding rust-resistant wheat varieties.

The invention belongs to the technical field of plant genetic engineering and crop breeding, and particularly relates to application of wheat lectin receptor-like kinase TaSIT2 and a coding gene TaSIT2 thereof in improving stripe rust resistance of crops. A wheat lectin receptor-like kinase gene TaSIT2 is analyzed and screened by using a reverse genetics method, a TaSIT2 gene editing wheat plant is obtained by using an agrobacterium-mediated genetic transformation method through a gene editing technology, the resistance of the transgenic plant to wheat stripe rust physiological races is remarkably enhanced, and the wheat stripe rust physiological races can be obtained. It is shown that knockout of the TaSIT2 gene enhances the resistance of wheat to stripe rust, it is determined that the wheat lectin receptor-like kinase TaSIT2 gene plays a negative regulation role in wheat stripe rust resistance, and a new technical thought and gene resources are provided for wheat stripe rust resistance variety cultivation from the perspective of molecular biology. And more paths are provided for cultivating lasting broad-spectrum disease-resistant varieties.

The application relates to application of a wheat stripe rust genome SNP site combination in wheat stripe rust gene typing, wherein the wheat stripe rust genome SNP site combination comprises 9365 SNP sites, the physical positions of the 9365 SNP sites are determined based on wheat stripe rust reference genome Pst_104E_v13_all_ctg alignment; and the physical position information of the 9365 SNP sites is shown in Table 1. The application can efficiently, conveniently, accurately, economically and rapidly perform wheat stripe rust gene typing detection with high coverage.

The invention discloses an SNP molecular marker related to wheat stripe rust and application of the SNP molecular marker, and relates to the technical field of molecular biology. In order to solve the technical problem of how to quickly and accurately identify the wheat stripe rust resistance, the invention provides the application of a substance for detecting the polymorphism or genotype of SNP in a wheat genome, the SNP is the 101st nucleotide of SEQ ID NO: 1 in a sequence table, and the nucleotide variety is T or C. The invention further provides the application of the substance for detecting the polymorphism or genotype of the SNP in the wheat genome. The invention also provides a composition and / or a kit for detecting the SNP. The method established by the invention can be used for distinguishing a disease-resistant material from a susceptible material, and provides an effective molecular marking tool for subsequent disease-resistant breeding. A molecular marker detection tool can be provided for identification of stripe rust-resistant germplasm resources and breeding of disease-resistant varieties, the selection efficiency is improved, and the breeding process is accelerated.

The invention belongs to the field of genetic engineering, and particularly relates to a wheat stripe rust resistance germplasm resource identification and breeding method. The CDS nucleotide sequence of the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 2. The developed functional molecular marker Yrym112-InDel comprises an upstream primer sequence as shown in SEQ ID NO. 3 and a downstream primer sequence as shown in SEQ ID NO. 4. A material containing the Yrym112 gene can be divided into a disease-resistant genotype and a susceptible genotype according to the size of an amplified band. The molecular marker is used for identifying or breeding wheat stripe rust resistant germplasm resources, or wheat gene Yr5 typing and wheat molecular marker assisted breeding are used, and the breeding process of wheat disease resistant varieties is accelerated.

This invention discloses a molecular breeding method for creating new wheat germplasm highly resistant to Fusarium head blight, stripe rust, and leaf rust. This method first develops newly located loci for grain weight / grain length / grain width. QTGW / KL / KW-1A The linkage marker KASP.QTGW / KL / KW-1A was identified and its effectiveness was verified. A major active site for resistance to Fusarium head blight was also developed. QFhb-2DL The linkage markers Fhb-2D-dcaps for the candidate genes were used. During the breeding process, molecular marker selection was first used to ensure that the created DH lines carried... QFhb-2DL Candidate genes for resistance to Fusarium head blight and QTGW / KL / KW-1A The enhanced allelic variation ensures the basic resistance and yield traits of the bred germplasm or materials. Furthermore, after propagation of the DH line, phenotypic identification was conducted in Yangzhou and Chengdu, simultaneously screening for resistance to Fusarium head blight, stripe rust, and leaf rust. Using local yield control varieties as comparisons, this facilitates the selection of germplasm or varieties adapted to local conditions and resistant to both Fusarium head blight and stripe rust / leaf rust, achieving a breakthrough in disease-resistant wheat breeding in the middle and upper reaches of the Yangtze River.

The application discloses application of ATG9-4B protein and a coding gene thereof in regulating disease resistance of wheat. The ATG9-4B protein is any one of the following B1)-B4): B1) a protein with an amino acid sequence shown in sequence 2; B2) a fusion protein with the same function obtained by connecting a tag to the N terminal and / or C terminal of the amino acid sequence shown in sequence 2; B3) a protein with the same function obtained by substituting, deleting and / or adding one or more amino acid residues to the amino acid sequence shown in sequence 2; and B4) a protein with 80% or above identity with the amino acid sequence shown in sequence 2 and with the same function. The application finds that the ATG9-4B protein and the coding gene thereof can regulate wheat stem rust resistance, and will play an important role in cultivating plant varieties with disease resistance.

This invention discloses the cloning and application of the wheat broad-spectrum multi-effect disease resistance gene WAI4. This invention provides a protein, which is as follows: A1) or A2) or A3): A1) a protein comprising the amino acid sequence shown in SEQ ID No. 2; A2) a protein derived from A1) by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in SEQ ID No. 2, and having the same function; A3) a fusion protein obtained by adding a tag protein to the end of the protein shown in A1). This invention provides gene localization, map-based cloning, and biological function identification methods for the wheat stress resistance gene WAI4, demonstrating that the gene WAI4 has resistance to powdery mildew and / or stripe rust, playing an important role in improving and enhancing germplasm resources of crops such as wheat.

The application discloses a wheat stripe rust grading recognition method based on an improved YOLOv11 and a ConvNeXtV2 model, and steps include: acquiring image data of a typical wheat stripe rust epidemic area and preprocessing; performing leaf boundary box labeling, and optimizing and training a leaf area extraction model YOLOv11-leaf; inputting a field wheat original image, and extracting a wheat leaf area image by using the trained leaf area extraction model YOLOv11-leaf; based on disease label labeling, performing wheat stripe rust multi-level severity recognition on the extracted wheat leaf area image by using a stripe rust grading model SE-ConvNeXtV2-Rust, and outputting a wheat stripe rust severity recognition result. The significant effect is that the light detection and efficient classification strategy are fused, and the balance between precision, resource efficiency and model interpretability is realized.

The invention belongs to the technical field of genetic engineering breeding, and discloses a wheat disease-resistant factor gene TaPsaF and application thereof. A TaPsaF gene sequence is analyzed, and a TaPsaF-GFP fusion protein is constructed, so that the localization of the TaPsaF-GFP fusion protein in cell chloroplast is determined. An overexpression and silence vector of the TaPsaF gene is successfully constructed by using a gene engineering technology, and wheat is transformed by using an agrobacterium-mediated method to obtain a transgenic wheat material. Functional verification shows that over-expression of the TaPsaF gene can significantly enhance the resistance of wheat to stripe rust, and silencing of the gene leads to reduction of the resistance. Gene resources and a technical path are provided for wheat stripe rust resistance molecular breeding, the blank of the TaPsaF protein in biological stress response mechanism research is filled up, and the TaPsaF protein has important significance for guaranteeing food safety.