20 results about "Stripe rust" patented technology

The application discloses a kind of molecular markers of wheat stripe rust SNP homozygous site based on whole genome sequence and application thereof.The application is analyzed to 28 strains of global wheat stripe rust genome sequence, compared with reference genome, and more than a million SNP sites are mined out.Sequence depth screening, linkage disequilibrium analysis and heterozygosity detection, identify 1076 SNP homozygous sites.Further based on the frequency of secondary allele, screening of deletion rate and adjacent simple repeat sequence, finally determine 37 core SNP markers.Through primer design to these SNP sites, and add fluorescent linker, develop KASP-SNP molecular marker, which can be used for accurate genotyping identification of wheat stripe rust population.The molecular marker developed based on global wheat stripe rust whole genome is suitable for wheat stripe rust population research in all regions of the world.Based on the development of molecular marker of homozygous site, polymorphism difference caused by heterozygous site can be effectively excluded, to ensure the accuracy of detection site.The set of KASP-SNP molecular marker has high polymorphism, good repeatability and high detection efficiency, and can be widely applied to genetic research of wheat stripe rust population.

This invention relates to the field of crop breeding technology, specifically to a highly efficient breeding method for early-maturing highland barley. The method includes the following steps: parent selection and matching, planting substrate preparation, application of growth regulators, planting management, multi-generation screening, and variety identification. This invention, by screening parents with early-maturing and disease-resistant characteristics, optimizing the planting substrate (adding microbial agents), precisely applying growth regulators, and dynamically controlling the planting environment and nutrient solution, combined with early maturity, disease resistance, and variety identification, efficiently breeds highland barley with a short growth period and strong resistance to stripe rust and powdery mildew. It optimizes plant type and yield structure, improves grain quality, achieves increased yield and genetic stability, and provides technical support for highland barley variety improvement and high-yield, high-quality cultivation.

This invention belongs to the field of genetic engineering technology and discloses... TaRNS4 Application of genes in wheat resistance to stripe rust. TaRNS4 The nucleotide sequence of the gene is shown in SEQ.ID.NO:1. This gene is involved in wheat resistance to stripe rust and plays a positive regulatory role in wheat resistance to stripe rust. Overexpression of the gene... TaRNS4 Genes enhance wheat's resistance to stripe rust. Overexpression TaRNS4 The gene can activate the wheat MAPK signaling pathway, increase the expression of disease resistance-related genes in wheat, thereby improving wheat's resistance to stripe rust. Overexpression TaRNS4 The gene can also reduce yield loss in wheat caused by stripe rust. This invention provides a germplasm resource for the green control of wheat stripe rust and offers new ideas for breeding rust-resistant wheat varieties.

This invention discloses a molecular breeding method for creating new wheat germplasm highly resistant to Fusarium head blight, stripe rust, and leaf rust. This method first develops newly located loci for grain weight / grain length / grain width. QTGW / KL / KW-1A The linkage marker KASP.QTGW / KL / KW-1A was identified and its effectiveness was verified. A major active site for resistance to Fusarium head blight was also developed. QFhb-2DL The linkage markers Fhb-2D-dcaps for the candidate genes were used. During the breeding process, molecular marker selection was first used to ensure that the created DH lines carried... QFhb-2DL Candidate genes for resistance to Fusarium head blight and QTGW / KL / KW-1A The enhanced allelic variation ensures the basic resistance and yield traits of the bred germplasm or materials. Furthermore, after propagation of the DH line, phenotypic identification was conducted in Yangzhou and Chengdu, simultaneously screening for resistance to Fusarium head blight, stripe rust, and leaf rust. Using local yield control varieties as comparisons, this facilitates the selection of germplasm or varieties adapted to local conditions and resistant to both Fusarium head blight and stripe rust / leaf rust, achieving a breakthrough in disease-resistant wheat breeding in the middle and upper reaches of the Yangtze River.

The application discloses application of ATG9-4B protein and a coding gene thereof in regulating disease resistance of wheat. The ATG9-4B protein is any one of the following B1)-B4): B1) a protein with an amino acid sequence shown in sequence 2; B2) a fusion protein with the same function obtained by connecting a tag to the N terminal and / or C terminal of the amino acid sequence shown in sequence 2; B3) a protein with the same function obtained by substituting, deleting and / or adding one or more amino acid residues to the amino acid sequence shown in sequence 2; and B4) a protein with 80% or above identity with the amino acid sequence shown in sequence 2 and with the same function. The application finds that the ATG9-4B protein and the coding gene thereof can regulate wheat stem rust resistance, and will play an important role in cultivating plant varieties with disease resistance.

This invention discloses the cloning and application of the wheat broad-spectrum multi-effect disease resistance gene WAI4. This invention provides a protein, which is as follows: A1) or A2) or A3): A1) a protein comprising the amino acid sequence shown in SEQ ID No. 2; A2) a protein derived from A1) by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in SEQ ID No. 2, and having the same function; A3) a fusion protein obtained by adding a tag protein to the end of the protein shown in A1). This invention provides gene localization, map-based cloning, and biological function identification methods for the wheat stress resistance gene WAI4, demonstrating that the gene WAI4 has resistance to powdery mildew and / or stripe rust, playing an important role in improving and enhancing germplasm resources of crops such as wheat.

The application discloses a wheat stripe rust grading recognition method based on an improved YOLOv11 and a ConvNeXtV2 model, and steps include: acquiring image data of a typical wheat stripe rust epidemic area and preprocessing; performing leaf boundary box labeling, and optimizing and training a leaf area extraction model YOLOv11-leaf; inputting a field wheat original image, and extracting a wheat leaf area image by using the trained leaf area extraction model YOLOv11-leaf; based on disease label labeling, performing wheat stripe rust multi-level severity recognition on the extracted wheat leaf area image by using a stripe rust grading model SE-ConvNeXtV2-Rust, and outputting a wheat stripe rust severity recognition result. The significant effect is that the light detection and efficient classification strategy are fused, and the balance between precision, resource efficiency and model interpretability is realized.

The application belongs to the technical field of genetic engineering and molecular breeding, and particularly relates to application of TaHPPD protein in regulation of wheat stripe rust resistance. Moro The TaHPPD protein can weaken active oxygen (ROS) accumulation and cell necrosis reaction triggered by CNL class protein NLR, and reduce ion leakage. The application obtains TaHPPD knockout (TaHPPD-KO) wheat material by knocking out TaHPPD gene in wheat through gene editing technology, and obtains wheat with wheat stripe rust resistance. The results of the embodiment show that, compared with the control Fielder, the TaHPPD-KO wheat plant shows significant resistance. The TaHPPD protein can be used to regulate wheat stripe rust resistance, and can be used for cultivating wheat resistant to stripe rust.

This invention discloses TaALDH7 Application of genes in regulating crop resistance to stripe rust and breeding disease-resistant varieties. This invention screened key genes regulating wheat resistance to stripe rust through combined transcriptomic and metabolomic analysis. TaALDH7 Through transient overexpression and gene silencing technology, TaALDH7 The function of the gene in wheat resistance to stripe rust was analyzed; the experimental results showed that, compared with the control group, the gene was overexpressed in wheat. TaALDH7 The disease index and fungal biomass of the gene were significantly reduced, and the colony area and hyphal length of stripe rust fungus were significantly decreased; conversely, in silent wheat... TaALDH7 The gene significantly reduces the resistance response of wheat to stripe rust, indicating that... TaALDH7 Genes act as positive regulators in the wheat's resistance to stripe rust. This invention shows promise for applications in breeding stripe rust-resistant wheat varieties and improving wheat's resistance to stripe rust fungi.

This invention discloses the disease resistance function and application of the CC domain of CNL protein 2541 involved in wheat stripe rust resistance, belonging to the field of functional gene technology. This invention provides the application of the 2541-CC gene domain in regulating wheat stripe rust resistance, and provides a recombinant expression vector overexpressing the 2541-CC gene domain, as well as a method for constructing plant germplasm overexpressing the 2541-CC gene domain using the recombinant expression vector. This invention found that plants overexpressing the 2541-CC gene domain exhibited significant resistance, increased leaf hypersensitive necrosis, reduced stripe rust urediniospores, increased reactive oxygen species, increased cell necrosis, and inhibited stripe rust growth and development, thus confirming that the 2541-CC gene domain is a key domain in the 2541 gene's disease resistance signal transduction.

The application discloses a method for improving resistance to wheat scab QFhb-2DL and QFhb- 3BL The application discloses a method for improving resistance to wheat scab, a molecular marker, a primer, the molecular marker comprising a dCAPS marker of QFhb-2DL and / or a KASP marker of QFhb-3BL . The application first develops a molecular marker closely linked to QFhb-2DL and a molecular marker closely linked to QFhb-3BL . Under the premise of stabilizing the resistance to stripe rust, dwarf, large spike and multiple spike characteristics of a wheat variety in the upper reaches of the Yangtze River, the application realizes molecular design breeding by using the developed new marker closely linked to the locus, and quickly introduces the disease-resistant genotypes of QFhb-2DL and QFhb-3BL into the wheat variety in the upper reaches of the Yangtze River, breeds a strain which is resistant to scab and has a yield significantly higher than that of a control, and realizes a breakthrough in the resistance to scab in the upper reaches of the Yangtze River.

A wheat E3 ubiquitin ligase gene RING-finger E3 ligase 1 (RFEL1) and its application in wheat disease resistance. The nucleotide sequence of the wheat E3 ubiquitin ligase gene RFEL1 is as shown in SEQ ID NO. 3. The present disclosure further provides an application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof, including any one of the following applications: A1, enhancing wheat resistance to stripe rust; A2, enhancing wheat resistance to powdery mildew; and A3, enhancing wheat resistance to leaf rust.

The present application belongs to the technical field of bioengineering, and relates to serine / threonine protein kinase TaPIX7-NW and application thereof. The amino acid sequence of the serine / threonine protein kinase TaPIX7-NW provided by the present application is shown as SEQ ID NO:1, and the coding ORF sequence of the serine / threonine protein kinase TaPIX7-NW is shown as SEQ ID NO:2. The present application performs gene editing on Fielder wild type material, obtains a TaPIX7-NW gene editing mutant plant, and verifies that the TaPIX7-NW gene editing mutant plant shows resistance to Puccinia striiformis CYR23. The present application verifies that the TaPIX7-NW gene is induced by the non-compatible race CYR23 of Puccinia striiformis, determines that the gene plays a positive regulation role in the resistance of wheat to stripe rust, and provides a new gene resource for wheat breeding against stripe rust.

This invention belongs to the field of genetic engineering breeding technology and provides a TaSnRK2.8 The application of genes in regulating wheat disease resistance. TaSnRK2.8 The nucleotide sequence of the gene is shown in SEQ ID NO:1. This gene encodes wheat cytoplasmic serine / threonine protein kinase TaSnRK2.8, ​​and the amino acid sequence of TaSnRK2.8 is shown in SEQ ID NO:2. Overexpression TaSnRK2.8 Genes can significantly enhance wheat's resistance to stripe rust and weaken the pathogenicity of stripe rust fungus; knockout TaSnRK2.8 The gene weakens wheat's resistance to disease, confirming that it is positively regulating wheat resistance to stripe rust. This invention provides important genetic resources and technical support for the genetic improvement of wheat resistance to rust, and can be used to breed wheat varieties resistant to stripe rust.

The application belongs to the field of bio-agriculture, and particularly relates to a wheat stripe rust effector protein gene HASP 38 and application thereof in improving wheat resistance to stripe rust. The amino acid sequence of the wheat stripe rust effector protein HASP 38 provided by the application is shown as SEQ ID NO: 1, the nucleotide sequence of the wheat stripe rust effector protein gene is shown as SEQ ID NO: 2, and an RNA interference vector of the gene is provided. HASP 38 The application transforms the RNA interference vector of the gene into Fielder wild-type material to obtain a gene-silenced wheat which exhibits resistance to stripe rust CYR32. HASP 38 The application determines that the wheat stripe rust effector protein gene HASP 38 plays a negative regulation role in wheat resistance to stripe rust, and uses the negative regulation factor to create a wheat material resistant to stripe rust, thereby providing a new gene and germplasm resource for wheat breeding against stripe rust.

This invention belongs to the field of plant genetic engineering technology, specifically relating to stripe rust fungus effector protein genes. Ps13167 The invention relates to the application of its encoded proteins in regulating plant resistance to stripe rust. This invention utilizes an Agrobacterium-mediated wheat genetic transformation system to transfer stripe rust effector protein genes... Ps13167 The silent and full-length fragments of the gene were transformed into wheat embryos, resulting in gene-silenced plants and overexpressing plants, respectively. Phenotypic identification revealed that the silent plants exhibited significant resistance to wheat stripe rust, while the overexpressing plants showed weakened resistance to stripe rust. Furthermore, this invention demonstrates the effectiveness of the stripe rust effector protein gene... Ps13167 Its important role in wheat infection by stripe rust and the ability to utilize stripe rust effector protein genes Ps13167 Improve the disease resistance of wheat, so as to breed genetic material resistant to rust.

The application belongs to the technical field of genetic engineering, and relates to a wheat stripe rust resistance protein YR26, a coding gene thereof and application. The wheat stripe rust resistance protein YR26 provided by the application has an amino acid sequence as shown in SEQ ID NO:1, and is encoded by a gene YR26 The coding sequence of the gene YR26 Is shown in SEQ ID NO:2. The sequence of the gene YR26 Is isolated for the first time, and the disease resistance function thereof is verified by various molecular biology means such as mutants, gene editing, transgenesis, gene silencing and the like.

The present application relates to the field of crop breeding technology, and specifically discloses a wheat tandem translocation chromosome, a molecular marker thereof and application, wherein the translocation chromosome comprises a segment of 637-812 Mb at the end of the long arm of a wheat 2B chromosome replaced by the long arm of a rye 2R chromosome and the 2S chromosome of a variable goat grass v to form a 2BS.2BL-2RL-2S v L tandem translocation chromosome. The present application creates a tandem translocation chromosome containing a powdery mildew resistance gene Pm2R QL and a stripe rust resistance gene YrAev , and develops a supporting molecular marker, solves the problem that after endogenous disease resistance genes are aggregated in wheat breeding, the identification of exogenous chromosome fragments relies on time-consuming and laborious cytological methods and is difficult to be applied in high-throughput, and quickly and accurately distinguishes between 2BS.2BL-2RL-2S v L tandem translocation chromosome carriers and non-carriers, replaces traditional cytological identification, supports molecular marker-assisted breeding, and accelerates the breeding process of new varieties of multi-resistant wheat.

This invention discloses TaMP Genes and their application in improving wheat rust resistance belong to the field of plant biotechnology and breeding. TaMP The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention utilizes Agrobacterium-mediated wheat embryo genetic transformation technology to prepare... TaMP Transgenic wheat plants with gene overexpression and knockout. It was verified that overexpression... TaMP Genes that reduce wheat's resistance to stripe rust; knockout TaMP The gene enhances wheat's resistance to stripe rust. Therefore, this invention verifies the efficacy of wheat myristylated modified protein. TaMP Genes play a crucial role in wheat's resistance to stripe rust. As mentioned above, TaMP This gene plays a negative regulatory role in the defense response of wheat against rust. It can be used to improve the disease resistance traits of wheat and other crops, and to breed new transgenic plant varieties resistant to disease.