191 results about "Stripe rust" patented technology

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat magnesium transporter TaNIPA8-6B and application of a coding gene thereof to regulation and control of plant stripe rust resistance. Functional analysis is carried out on the wheat magnesium transporter TaNIPA8-6B by means of gene editing and overexpression, and it is found that the wheat magnesium transporter TaNIPA8-6B induces expression in both affinity and non-affinity system reactions of wheat and puccinia striiformis, so that a mutant plant of the gene editing and overexpression TaNIPA8-6B gene is created; according to the TaNIPA8-6B gene, the gene knockout mutant of the TaNIPA8-6B gene is identified to show susceptibility to stripe rust non-compatible races CYR23, the over-expression mutant plant shows disease resistance to stripe rust compatible races CYR32, the fact that the TaNIPA8-6B gene plays a positive regulation role in the disease resistance of wheat to stripe rust is clear, and a high-quality germplasm resource is provided for stripe rust resistance of wheat.

The invention provides wheat receptor cytoplasm kinase TaRIPK8 and application thereof in improvement of stripe rust resistant varieties of cereal crops, and belongs to the technical field of biological agriculture. The invention provides application of wheat receptor cytoplasm kinase TaRIPK8 in regulation and control of stripe rust resistance of cereal crops and / or improvement or cultivation of stripe rust resistance varieties of the cereal crops. The wheat receptor cytoplasm kinase TaRIPK8 comprises protein with an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The invention further provides application of the wheat receptor cytoplasm kinase TaRIPK8 in regulation and control of the stripe rust resistance character of the cereal crops and / or improvement or cultivation of the stripe rust resistance varieties of the cereal crops. The receptor-like cytoplasm kinase TaRIPK8 participates in negative regulation of wheat on stripe rust, so that a high stripe rust resistance strain can be created on the basis, and an excellent wheat material is provided for cultivation of stripe rust resistance varieties.

The invention relates to the technical field of intelligent agricultural remote sensing monitoring, in particular to a wheat stripe rust multi-scale detection system and method based on unmanned aerial vehicle multi-spectral images. Comprising an attitude compensation image registration module, a sheath and leaf boundary enhancement module, a stripe rust incubation period stripe extraction module, a canopy stomatal density inversion and mapping module and a three-dimensional space infection simulation module. Wherein the attitude compensation image registration module is used for eliminating flight attitude interference; the leaf sheath and leaf junction enhancing module is used for enhancing the structural boundary characteristics of a leaf sheath and leaf junction area; the stripe rust incubation period stripe extraction module is used for extracting disease incubation period stripe features; and the canopy stomatal density inversion and mapping module is used for inverting canopy stomatal conductance parameters. According to the method, by fusing multispectral image feature extraction, thermal infrared stomatal conductance inversion and three-dimensional space propagation modeling, multi-scale accurate detection and visual expression of the wheat stripe rust from incubation period identification to a spatial diffusion process are realized.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention relates to the technical field of molecular genetics, and particularly discloses a Yr6 gene, protein, a recombinant vector and application of the Yr6 gene, the protein and the recombinant vector, the full-length sequence of the Yr6 gene is shown as SEQ ID NO.1, and the CDS sequence of the Yr6 gene is shown as SEQ ID NO.2. Yr6 is overexpressed in a transgenic mode to improve wheat stripe rust resistance. The Yr6 gene provided by the invention is beneficial to improving the resistance of wheat to stripe rust, and provides support for breeding of stripe-rust-resistant wheat.

The invention belongs to the technical field of biology, and particularly relates to a TaGAT2-1B gene resistant to wheat stripe rust and application of the TaGAT2-1B gene, and the nucleotide sequence of the TaGAT2-1B gene is shown as SEQ ID NO.3. It is found that the TaGAT2-1B gene and the encoding protein thereof can effectively improve the wheat stripe rust resistance, the TaGAT2-1B gene is a new stripe rust resistance gene in the wheat adult-plant stage, infection of various stripe rust strains can be effectively resisted, the field phenotype of the TaGAT2-1B gene shows the characteristic of slow rust, and the TaGAT2-1B gene and the encoding protein thereof have good application prospects. The discovery of the gene provides a new genetic resource for the research of the wheat stripe rust resistance mechanism and the wheat stripe rust resistance breeding.

The invention provides application of wheat cysteine receptor kinase TaCRK25-1B and a coding gene thereof in regulation and control of stripe rust resistance of plants, and belongs to the technical field of plant genetic engineering. The TaCRK25-1B provided by the invention is subjected to induced expression of stripe rust infection in wheat, a VIGS technology instantaneous silencing experiment shows that the resistance of the wheat to stripe rust CYR31 is enhanced by silencing the TaCRK25-1B, the TaCRK25-1B is over-expressed in the wheat by a genetic transformation technology, the resistance of the wheat to stripe rust is weakened, and an RNAi interference plant infection pathogen experiment of the TaCRK25-1B created by an RNAi technology shows that the TaCRK25-1B has the advantages that the TaCRK25-1B can be used for expressing the stripe rust CYR31 in the wheat; the TACRK25-1B plays a negative regulation role in wheat stripe rust resistance. Therefore, the invention provides a new means for wheat stripe rust resistance and resistant wheat breeding.

The invention discloses a terahertz time-domain spectroscopy-based wheat stripe rust uredospore optical parameter extraction method. The method comprises the following steps: mixing wheat stripe rust uredospore powder and polyethylene powder, and tabletting; a transmission module of the terahertz time-domain spectrometer is used for collecting time-domain spectral data of the mixed tablets in a nitrogen environment; carrying out filtering processing on the collected time-domain spectral data to eliminate high-frequency noise; main waves of the time domain signals are reserved, the echo position is estimated, sampling points are randomly generated through normal distribution to dynamically correct the echo signals, and time domain interference is reduced; and performing fast Fourier transform on the corrected time domain signal, and calculating the refractive index, the absorption coefficient and the dielectric constant of the wheat stripe rust uredospores through frequency domain amplitude and phase information on the basis of an improved Dorne-Duvillaret model. Through sample preparation optimization, echo correction and model improvement, the measurement precision can be remarkably improved.

The invention belongs to the field of biological agriculture, and particularly relates to application of a wheat stripe rust effect protein gene HASP 38 in improvement of stripe rust resistance of wheat. The amino acid sequence of the wheat stripe rust effect protein HASP 38 provided by the invention is as shown in SEQ ID NO: 1, the nucleotide sequence of the wheat stripe rust effect protein gene HASP 38 is as shown in SEQ ID NO: 2, and an RNA interference vector of the gene is provided. According to the invention, an RNA (Ribonucleic Acid) interference vector of the gene is transformed into a Fielder wild type material, so that the HASP 38 gene silencing wheat with resistance to stripe rust CYR32 is obtained. According to the invention, the wheat stripe rust effect protein gene HASP 38 is determined to play a negative regulation role in wheat stripe rust resistance, and a stripe rust resistant wheat material is created by using the negative regulation factor, so that a new gene and germplasm resource are provided for wheat stripe rust resistant breeding.

The invention discloses a wheat stripe rust molecular marker and application thereof in stripe rust resistance identification. The wheat stripe rust molecular marker disclosed by the invention is nucleotides corresponding to 29th to 32th sites of SEQ ID No.2 in a wheat genome, and the nucleotides are 29th to 32th sites of SEQ ID No.2 or 29th to 32th sites of SEQ ID No.2 deleted. Experiments prove that wheat materials with stripe rust resistance can be quickly screened out by applying the molecular marker disclosed by the invention, so that the breeding pace of new wheat varieties is accelerated. The method has important theoretical significance and economic value.

The invention belongs to the technical field of gene engineering, and discloses application of a transcription factor TaNAC67 in regulation and control of wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown as SEQ ID NO: 1, and the transcription factor can induce cell necrosis and exert a positive regulation function in the interaction process of wheat and puccinia striiformis. The coding gene TaNAC67 of the transcription factor is a disease-resistant related gene, is infected by stripe rust in non-affinity interaction of plants and stripe rust and is strongly induced to express, and the nucleotide sequence is shown as SEQ ID NO: 2. On the basis of the characteristics, a transgenic plant over-expressing the TaNAC67 gene is further obtained, and it is verified that the transgenic plant shows resistance to stripe rust. The invention provides gene resources and technical support for subsequent genetic improvement of stripe rust resistant wheat materials.

The invention discloses a KASP molecular marker related to wheat stripe rust resistance characters and application of the KASP molecular marker, and belongs to the technical field of molecular markers and plant genetic breeding. SNP sites QYr.hzau4B of the KASP molecular marker are located at the 452, 424 and 208 basic groups of a wheat reference genome 4B chromosome, C / T mutation exists at the sites, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO. 1. The KASP molecular marker can be used for identifying stripe rust resistant and susceptible varieties or strains of wheat, and a valuable molecular tool is provided for screening stripe rust resistant germplasm resources of wheat, so that the breeding efficiency of improved varieties is improved, and the breeding process is accelerated.

Compositions and methods for enhancing the resistance of wheat and barley plants to wheat stripe rust caused by Puccinia striiformis f. sp. tritici are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of wheat and barley plants to wheat stripe rust comprise introducing a nucleic acid molecule encoding an R gene product into a wheat or barley plant cell. Additionally provided are methods for using the wheat and barley plants in agriculture to limit wheat stripe rust.

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.

The utility model discloses wheat stripe rust collecting and storing equipment which comprises a handle, a collector is fixedly connected to the top of the handle, a collecting tank is detachably connected to the bottom of the collector, a material suction opening is fixedly connected to one end of the collector, and a collecting assembly is arranged on the outer wall of the material suction opening. According to the wheat stripe rust fungus collecting and storing equipment, materials can be sucked in through a material suction opening, in the suction process, wheat leaves can be held by the other hand to be straightened, a brush is attached to the wheat leaves, rust fungi on the surfaces of the wheat leaves can be treated through back-and-forth pulling, in addition, when the brush conducts brushing, the wheat leaves can be conveniently cleaned, and the wheat leaves can be conveniently stored. Under the supporting of the supporting spring, the connecting rod can move back and forth through the connecting plate, through the arrangement of the supporting spring, the flexibility, adaptability and durability of the brush can be enhanced, the spring can ensure that the brush and the surface keep proper contact force for uneven wheat leaves, and therefore the material suction effect is improved.

The application discloses a kind of molecular markers of wheat stripe rust SNP homozygous site based on whole genome sequence and application thereof.The application is analyzed to 28 strains of global wheat stripe rust genome sequence, compared with reference genome, and more than a million SNP sites are mined out.Sequence depth screening, linkage disequilibrium analysis and heterozygosity detection, identify 1076 SNP homozygous sites.Further based on the frequency of secondary allele, screening of deletion rate and adjacent simple repeat sequence, finally determine 37 core SNP markers.Through primer design to these SNP sites, and add fluorescent linker, develop KASP-SNP molecular marker, which can be used for accurate genotyping identification of wheat stripe rust population.The molecular marker developed based on global wheat stripe rust whole genome is suitable for wheat stripe rust population research in all regions of the world.Based on the development of molecular marker of homozygous site, polymorphism difference caused by heterozygous site can be effectively excluded, to ensure the accuracy of detection site.The set of KASP-SNP molecular marker has high polymorphism, good repeatability and high detection efficiency, and can be widely applied to genetic research of wheat stripe rust population.

The application provides application of a wheat ELP2 gene in regulating resistance of wheat to stripe rust, and belongs to the technical field of genetic engineering. The application provides application of the wheat ELP2 gene in regulating resistance of wheat to stripe rust, also provides a silencing fragment of the wheat ELP2 gene obtained by an amplification method, and gene silencing of the wheat ELP2 gene is carried out by using the silencing fragment, and in the examples, overexpression plants of the ELP2 gene are constructed, it is found that the overexpression weakens the resistance of wheat to non-compatible races of Puccinia striiformis f. sp. tritici, the interaction relationship between ELP2 and the PAZ motif of DCL1 is verified by using yeast double hybridization, LCI and Pull-down, meanwhile, the resistance of wheat to compatible races is enhanced and the infection of Puccinia striiformis f. sp. tritici is inhibited after silencing DCL1 in wheat by VIGS, therefore, the ELP2 gene plays a disease function in the interaction between wheat and Puccinia striiformis f. sp. tritici.

The invention provides a GMRM (Genome-based Mutant RNA Mapping) method taking a genome as a reference, in particular, the invention provides a method for cloning candidate functional genes of wheat stripe rust resistant plants and an analysis process, and the GMRM method disclosed by the invention can be used for simultaneously cloning two genes with complementary disease-resistant functions, namely Yr6NLR1 and Yr6NLR2. In an example of Yr6, the two disease-resistant genes are closely linked genetically, but the cloning process of the genes does not involve any genetic recombination. Therefore, theoretically, the method provided by the invention can be used for simultaneously cloning a plurality of functional complementary genes (namely genes participating in the same pathway) on the premise that the target gene can generate effective mutation through EMS mutagenesis.

The invention discloses a wheat stripe rust adult plant resistance gene Yr54, a specific functional molecular marker and application of the wheat stripe rust adult plant resistance gene Yr54 in wheat stripe rust resistance breeding, and belongs to the technical field of biology. A wheat stripe rust adult-plant resistance gene TaNLR2DL transgenic line is subjected to adult-plant-stage stripe rust resistance identification, and a result shows that compared with a non-TaNLR2DL gene line, the stripe rust resistance of the TaNLR2DL transgenic line is obviously improved, while a TaNLR2DL gene knockout line is contrary to the TaNLR2DL gene knockout line, so that the TaNLR2DL is a potential stripe rust resistance gene, and the TaNLR2DL gene knockout line can be used for identifying the stripe rust resistance in the adult-plant stage. The method can be used for genetic improvement of wheat stripe rust resistance. The invention further develops a functional specific molecular marker of TaNLR2DL, and the marker can quickly screen wheat germplasm containing TaNLR2DL, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

This invention relates to the field of crop breeding technology, specifically to a highly efficient breeding method for early-maturing highland barley. The method includes the following steps: parent selection and matching, planting substrate preparation, application of growth regulators, planting management, multi-generation screening, and variety identification. This invention, by screening parents with early-maturing and disease-resistant characteristics, optimizing the planting substrate (adding microbial agents), precisely applying growth regulators, and dynamically controlling the planting environment and nutrient solution, combined with early maturity, disease resistance, and variety identification, efficiently breeds highland barley with a short growth period and strong resistance to stripe rust and powdery mildew. It optimizes plant type and yield structure, improves grain quality, achieves increased yield and genetic stability, and provides technical support for highland barley variety improvement and high-yield, high-quality cultivation.

Provided are a strain of Bacillus velezensis EA19 and the use thereof. The strain is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO:M2023602. The strain has a relatively good treatment effect and prevention effect on stripe rust of Triticuma aestivum, and shows a comparable control efficacy to triadimefon. Moreover, the strain has an effect of reducing dosage while enhancing efficacy when used in combination with mefentrifluconazole. Therefore, the strain has important application values in the control of agricultural pests and diseases, provides a new way for green control of stripe rust of Triticuma aestivum, and has broad application prospects.

The invention discloses a molecular marker for detecting stripe rust and brown leaf rust resistant one-cause multiple-effect sites of wheat and a use method, and belongs to the technical field of nucleotide polymorphism and molecular markers. The technical problem to be solved by the invention is how to quickly and accurately screen or assist in screening wheat with stripe rust or / and leaf rust resistance or / and breed or assist in breeding wheat single plants with stripe rust or / and leaf rust resistance. The invention provides a composition for detecting single nucleotide polymorphism or genotype of a specific SNP site in a wheat genome, the SNP site can be the 47th nucleotide of a DNA fragment as shown in SEQ ID NO: 1 in the wheat genome, and the type of the nucleotide can be A or T. The primer composition and the molecular marker provided by the invention assist in wheat breeding for resisting stripe rust and leaf rust in wheat disease resistance breeding, greatly shorten the breeding period and play an important role in wheat breeding.

The invention relates to the technical field of biology, and particularly discloses application of a TaSLAH2-B gene or a TaSLAH2-B protein in improving wheat stripe rust resistance and a recombinant vector, the nucleotide sequence of the TaSLAH2-B gene is shown as SEQ ID NO.1, the CDS sequence of the TaSLAH2-B gene is shown as SEQ ID NO.2, and the amino acid sequence of the protein coded by the TaSLAH2-B gene is shown as SEQ ID NO.3. The invention further discloses a preparation method of the TaSLAH2-B gene. The TaSLAH2-B gene provided by the invention achieves the purpose of improving stripe rust resistance through overexpression in wheat plants.

The present invention discloses a seed endophyte for preventing and treating wheat fungal diseases and its application, belonging to the technical field of crop planting and crop disease biological control. The wheat endophyte (Pseudescherichia vulneris) GSE25 in the present invention was deposited in the China General Microbiological Culture Collection Center (CGMCC) on November 21, 2022, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC NO.26158. The GSE25 strain has the ability to significantly reduce the pathogenicity of gibberellins and stripe rust on wheat plants, and can significantly reduce the mycotoxins produced by pathogens on wheat grains, has a promoting effect on crop growth and development, and has a broad-spectrum effect. The GSE25 strain of the present invention is of great value in the healthy growth, yield increase, control of pathogenic fungi and mycotoxin pollution of crops.

The invention belongs to the technical field of molecular biology, and relates to a KASP molecular marker for wheat stripe rust resistance gene detection and application. The KASP molecular marker XK6B-5 disclosed by the invention is obtained by utilizing a specific primer group as shown in SEQ ID NO.2-4 for amplification; the KASP molecular marker XK6B-5 is closely linked with a wheat stripe rust resistance gene YrZT94.1, and has a co-separation characteristic, and the polymorphism of the XK6B-5 is G / T. The KASP molecular marker provided by the invention can accurately track the stripe rust resistance gene YrZT94.1 of wheat, performs molecular marker-assisted selection, and improves the breeding efficiency of wheat disease resistance. The method can be applied to screening of the stripe rust resistant gene YrZT94.1 in wheat germplasm resources in a simple, rapid and high-throughput manner, so that the method is applied to molecular design breeding, and the wheat breeding process is greatly accelerated.

The invention belongs to the technical field of molecular biology, and relates to a KASP molecular marker for detecting a wheat stripe rust resistance gene YrZT94.2 and application of the KASP molecular marker. The KASP molecular marker XK7B-32 disclosed by the invention is obtained by utilizing a specific primer group as shown in SEQ ID NO.2-4 for amplification; the KASP molecular marker XK7B-32 is closely linked with a wheat stripe rust resistance gene YrZT94.2, and has a co-separation characteristic, and the polymorphism of the XK7B-32 is C / G. The KASP molecular marker provided by the invention can accurately track the stripe rust resistance gene YrZT94.2 of wheat, performs molecular marker-assisted selection, and improves the breeding efficiency of wheat disease resistance. The method can be simply and quickly applied to screening of the stripe rust resistant gene YrZT94.2 in wheat germplasm resources with high throughput, so that the method is applied to molecular design breeding, and the wheat breeding process is greatly accelerated.

This invention belongs to the field of genetic engineering technology and relates to the application of wheat receptor kinase TaSPR12 in improving wheat disease resistance. This invention utilizes reverse genetics methods to analyze and screen wheat receptor kinase genes. TaSPR12 This gene is induced to express by the stripe rust pathogen. The wheat receptor kinase gene was knocked out using CRISPR-Cas9 gene editing technology. TaSPR12 The created loss-of-function mutants exhibited enhanced resistance to the main prevalent races of wheat stripe rust. This invention identified the wheat receptor kinase gene. TaSPR12 It plays a negative regulatory role in wheat resistance to stripe rust and can be used for genetic improvement of wheat resistance to rust.

The present invention relates to a method for monitoring summer spores of wheat stripe rust based on a spin Unet++ network, including: obtaining microscopic images of summer spores; constructing a sample data set; dividing the sample data set into a training set, a test set, and a validation set; improving the Unet++ network model to obtain an improved Unet++ network model; inputting the images in the sample data set into the improved Unet++ network model for spin training to obtain a summer spore counting network model, and outputting the images in the sample data set with detection frames; training the summer spore counting network model; inputting the microscopic images of the summer spores to be detected into the trained summer spore counting network model, and outputting images with detection frames and counts. In the present invention, the counting accuracy of the summer spore counting network model is high, reaching 99.03%; the segmentation rate is relatively high, reaching 86.45%; the detection rate is relatively high, reaching 14 images per second, and the memory occupancy is small, only 46.8 MB.

The present invention discloses KASP molecular markers linked to the wheat stripe rust resistance gene YrG363 and their applications, belonging to the field of agricultural biotechnology. The KASP molecular markers include Sicau-1BS.1 and Sicau-1BS.2, and their nucleotide sequences are as shown in SEQ ID NO.1-SEQ ID NO.2. The KASP molecular markers can be used for the fine mapping of YrG363 and molecular marker-assisted selection of the YrG363 gene, improving the efficiency of wheat disease-resistant breeding. The present invention overcomes the disadvantages in traditional disease-resistant breeding, such as the susceptibility of stripe rust resistance phenotype selection to environmental influence, poor stability and repeatability, and low efficiency, thus accelerating the breeding process of wheat varieties resistant to stripe rust. The KASP molecular markers do not rely on gel electrophoresis and can achieve high-throughput and large-scale detection of the YrG363 gene locus.