219 results about "Stripe rust" patented technology

The invention discloses application of a wheat WRKY transcription factor gene TaWRKY1, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the gene TaWRKY1 is shown as SEQ ID NO: 1, and the gene TaWRKY1 is infected by stripe rust to induce expression in interaction of wheat and stripe rust, and plays a negative regulation role in wheat stripe rust resistance. A TaWRKY1 gene editing mutant plant is created by using an agrobacterium tumefaciens-mediated genetic transformation method, the sporulation quantity on wheat leaves of the gene editing mutant is obviously reduced, and the allergic necrosis is increased, which indicates that the edited gene TaWRKY1 can improve the resistance of wheat to stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention provides a wheat early-stage stripe rust detection method and system, and the method comprises the steps: inputting time dimension embedded multi-modal data, pre-training a spectrum reconstruction and time sequence comparison learning model, and mining universal feature modes of wheat stripe rust in different development stages and different spectral bands; and transferring the pre-trained weight to a multi-modal encoder of a multi-modal multi-scale target detection model, extracting interactive fusion features of different scales, inputting the interactive fusion features into a multi-scale decoder, comprehensively utilizing global context information and local detail information, and meanwhile, fully utilizing the features of different scales through a cross attention mechanism so as to obtain a multi-scale multi-scale target detection model. Outputting a stripe rust detection result; finely adjusting parameters of the multi-scale decoder by using data with labels in the early-stage stripe rust data to obtain a trained multi-modal multi-scale target detection model; and performing early stripe rust detection on wheat to be detected by using the trained multi-modal multi-scale target detection model. The kit can be used for detecting the early wheat stripe rust.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention provides a wheat stripe rust early warning method, system and device, a storage medium and a program product, and relates to the technical field of agriculture, and the method comprises the steps: obtaining the spore capture amount, meteorological data and field morbidity of a to-be-monitored farmland; the to-be-monitored farmland is used for planting to-be-monitored wheat, the spore capture amount is the capture number of puccinia striiformis wheat specialized spores, and the field morbidity is the probability that the to-be-monitored wheat is infected with wheat stripe rust; based on the meteorological data, the spore capture amount and the field morbidity, constructing a wheat stripe rust prediction model; and based on the wheat stripe rust prediction model, performing wheat stripe rust monitoring and early warning on the to-be-monitored farmland. By means of the mode, efficient, accurate and real-time monitoring and early warning can be conducted on the wheat stripe rust without depending on manual investigation, the accuracy rate is high, the efficiency is high, and outbreak of the wheat stripe rust can be effectively prevented.

The invention belongs to the technical field of genetic engineering, and discloses a phytosusceptibility-related TaCNGC13 gene, a protein and application of the phytosusceptibility-related TaCNGC13 gene and the protein. The phytosusceptible related TaCNGC13 gene is derived from a wheat variety water source 11, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the gene encoding protein is shown as SEQ ID NO: 2. The plant-susceptibility-related TaCNGC13 gene is induced to express by the puccinia striiformis, the expression of the plant-susceptibility-related TaCNGC13 gene in wheat is stably silenced, and the resistance of the obtained transgenic wheat to a plurality of physiological races of the puccinia striiformis is higher than that of wild wheat. The invention provides a novel germplasm material for green control of wheat stripe rust and cultivation of wheat stripe rust resistant varieties.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat magnesium transporter TaNIPA8-6B and application of a coding gene thereof to regulation and control of plant stripe rust resistance. Functional analysis is carried out on the wheat magnesium transporter TaNIPA8-6B by means of gene editing and overexpression, and it is found that the wheat magnesium transporter TaNIPA8-6B induces expression in both affinity and non-affinity system reactions of wheat and puccinia striiformis, so that a mutant plant of the gene editing and overexpression TaNIPA8-6B gene is created; according to the TaNIPA8-6B gene, the gene knockout mutant of the TaNIPA8-6B gene is identified to show susceptibility to stripe rust non-compatible races CYR23, the over-expression mutant plant shows disease resistance to stripe rust compatible races CYR32, the fact that the TaNIPA8-6B gene plays a positive regulation role in the disease resistance of wheat to stripe rust is clear, and a high-quality germplasm resource is provided for stripe rust resistance of wheat.

The invention provides wheat receptor cytoplasm kinase TaRIPK8 and application thereof in improvement of stripe rust resistant varieties of cereal crops, and belongs to the technical field of biological agriculture. The invention provides application of wheat receptor cytoplasm kinase TaRIPK8 in regulation and control of stripe rust resistance of cereal crops and / or improvement or cultivation of stripe rust resistance varieties of the cereal crops. The wheat receptor cytoplasm kinase TaRIPK8 comprises protein with an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The invention further provides application of the wheat receptor cytoplasm kinase TaRIPK8 in regulation and control of the stripe rust resistance character of the cereal crops and / or improvement or cultivation of the stripe rust resistance varieties of the cereal crops. The receptor-like cytoplasm kinase TaRIPK8 participates in negative regulation of wheat on stripe rust, so that a high stripe rust resistance strain can be created on the basis, and an excellent wheat material is provided for cultivation of stripe rust resistance varieties.

The invention belongs to the technical field of gene engineering, and discloses a wheat susceptibility related gene, a protein and application. The wheat susceptibility related gene is a TaBRG10 gene and is derived from a wheat variety water source 11, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the TaBRG10 gene is as shown in SEQ ID NO: 2. The TaBRG10 gene is induced to express by stripe rust, and under the infection of stripe rust, the infection surface of a TaBRG10 gene silent plant infected by stripe rust is positively and obviously lower than that of a control plant, which indicates that the silent TaBRG10 gene improves the resistance of the plant to wheat stripe rust. The invention provides a novel germplasm material for the cultivation of the stripe rust resistant wheat variety.

The invention belongs to the technical field of biotechnology, and particularly relates to a wheat thermo-sensitive stripe rust resistant gene TaEDR2-7B, a recombinant vector, application and a method. The nucleotide sequence of the gene TaEDR2-7B is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention provides the gene TaEDR-7B related to the temperature-sensitive stripe rust resistance of the wheat as well as the encoding protein and the application of the gene TaEDR-7B for the first time. The invention discloses that the gene TaEDR2-7B plays an important role in the temperature-sensitive disease resistance of the wheat.

The invention relates to the technical field of intelligent agricultural remote sensing monitoring, in particular to a wheat stripe rust multi-scale detection system and method based on unmanned aerial vehicle multi-spectral images. Comprising an attitude compensation image registration module, a sheath and leaf boundary enhancement module, a stripe rust incubation period stripe extraction module, a canopy stomatal density inversion and mapping module and a three-dimensional space infection simulation module. Wherein the attitude compensation image registration module is used for eliminating flight attitude interference; the leaf sheath and leaf junction enhancing module is used for enhancing the structural boundary characteristics of a leaf sheath and leaf junction area; the stripe rust incubation period stripe extraction module is used for extracting disease incubation period stripe features; and the canopy stomatal density inversion and mapping module is used for inverting canopy stomatal conductance parameters. According to the method, by fusing multispectral image feature extraction, thermal infrared stomatal conductance inversion and three-dimensional space propagation modeling, multi-scale accurate detection and visual expression of the wheat stripe rust from incubation period identification to a spatial diffusion process are realized.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention provides a stripe rust resistant gene and application thereof. The stripe rust resistant gene is over-expressed in a material Yannong 19 with high stripe rust susceptibility, and a transgenic positive plant shows near immunity.

The invention discloses a wheat stripe rust resistance regulation gene TaWAK2 as well as an encoding protein and application thereof, and belongs to the technical field of molecular biology. The wheat stripe rust resistance regulation gene TaWAK2 disclosed by the invention is as shown in a sequence table SEQ ID NO: 1, and the length of the wheat stripe rust resistance regulation gene TaWAK2 is 7042bp; the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 4 in a sequence table, and the length of the amino acid sequence is 693. Through a VIGS silencing technology and gene sequencing analysis, it is proved that the gene TaWAK2 plays an important role in wheat stripe rust resistance regulation and control, and the wheat stripe rust resistance is negatively regulated and controlled. The gene TaWAK2 disclosed by the invention can be applied to wheat stripe rust resistance breeding; research on the gene is beneficial to revealing a molecular genetic basis of wheat stripe rust resistance, and an important gene target is provided for wheat breeding for disease resistance.

The invention relates to the technical field of molecular genetics, and particularly discloses a Yr6 gene, protein, a recombinant vector and application of the Yr6 gene, the protein and the recombinant vector, the full-length sequence of the Yr6 gene is shown as SEQ ID NO.1, and the CDS sequence of the Yr6 gene is shown as SEQ ID NO.2. Yr6 is overexpressed in a transgenic mode to improve wheat stripe rust resistance. The Yr6 gene provided by the invention is beneficial to improving the resistance of wheat to stripe rust, and provides support for breeding of stripe-rust-resistant wheat.

The invention belongs to the technical field of biology, and particularly relates to a TaGAT2-1B gene resistant to wheat stripe rust and application of the TaGAT2-1B gene, and the nucleotide sequence of the TaGAT2-1B gene is shown as SEQ ID NO.3. It is found that the TaGAT2-1B gene and the encoding protein thereof can effectively improve the wheat stripe rust resistance, the TaGAT2-1B gene is a new stripe rust resistance gene in the wheat adult-plant stage, infection of various stripe rust strains can be effectively resisted, the field phenotype of the TaGAT2-1B gene shows the characteristic of slow rust, and the TaGAT2-1B gene and the encoding protein thereof have good application prospects. The discovery of the gene provides a new genetic resource for the research of the wheat stripe rust resistance mechanism and the wheat stripe rust resistance breeding.

The invention discloses a wheat stripe rust effect protein gene Pst4593 and application thereof, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the wheat stripe rust effect protein gene Pst4593 is shown as SEQ ID NO: 1, the gene positively regulates pathogenicity of stripe rust to wheat, silence of the wheat stripe rust effect protein gene Pst4593 can improve resistance of wheat to stripe rust, and relative expression quantities of wheat related defense genes TaPR1, TaPR2 and TaPR5 are remarkably improved. The wheat stripe rust effect protein gene Pst4593 is related to induced expression of stripe rust aspirator, and the protein coded by the wheat stripe rust effect protein gene Pst4593 also has lipase activity and can influence pathogenicity of stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention provides application of wheat cysteine receptor kinase TaCRK25-1B and a coding gene thereof in regulation and control of stripe rust resistance of plants, and belongs to the technical field of plant genetic engineering. The TaCRK25-1B provided by the invention is subjected to induced expression of stripe rust infection in wheat, a VIGS technology instantaneous silencing experiment shows that the resistance of the wheat to stripe rust CYR31 is enhanced by silencing the TaCRK25-1B, the TaCRK25-1B is over-expressed in the wheat by a genetic transformation technology, the resistance of the wheat to stripe rust is weakened, and an RNAi interference plant infection pathogen experiment of the TaCRK25-1B created by an RNAi technology shows that the TaCRK25-1B has the advantages that the TaCRK25-1B can be used for expressing the stripe rust CYR31 in the wheat; the TACRK25-1B plays a negative regulation role in wheat stripe rust resistance. Therefore, the invention provides a new means for wheat stripe rust resistance and resistant wheat breeding.

The invention belongs to but not limited to the technical field of plant protection, and particularly relates to application and method for improving wheat stripe rust resistance through allogenic material treatment, 250 micromoles of luteolin is used for culturing stripe rust fungus spores, germination of the stripe rust fungus spores is remarkably inhibited, 500 micromoles of luteolin is used for pretreating wheat leaves, spraying is carried out once a day, spraying is carried out three times in total, and the yield of the wheat stripe rust resistance is increased. After the leaves absorb the luteolin, the puccinia striiformis is inoculated, and the result shows that the endogenous luteolin content of the wheat plants treated by the luteolin is increased, the infection area of the puccinia striiformis is remarkably reduced, the number of puccinia striiformis spores on the leaves is remarkably reduced, and the biomass of the puccinia striiformis is remarkably reduced. The invention proves that the occurrence of wheat stripe rust can be inhibited by exogenous luteolin treatment, and a certain reference is provided for stripe rust disease-resistant mechanism research and prevention and control measures.

The invention relates to the field of biomolecular detection, in particular to a KASP molecular marker related to wheat stripe rust resistance characters, a primer pair and application of the KASP molecular marker and the primer pair. According to the invention, 559 parts of common wheat germplasm resources are subjected to whole genome association analysis, a stable stripe rust resistance site QYr.hzau-3B. 1 is identified on a wheat 3B chromosome long arm, and a KASP molecular marker (KASP molecular marker KASP 3B2800) is developed aiming at the site and is used for screening large-scale materials. The KASP molecular marker provided by the invention provides a new site and a new marker for wheat stripe rust resistance breeding; meanwhile, the KASP molecular marker can be used for rapidly screening wheat germplasm containing the site, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The invention discloses a terahertz time-domain spectroscopy-based wheat stripe rust uredospore optical parameter extraction method. The method comprises the following steps: mixing wheat stripe rust uredospore powder and polyethylene powder, and tabletting; a transmission module of the terahertz time-domain spectrometer is used for collecting time-domain spectral data of the mixed tablets in a nitrogen environment; carrying out filtering processing on the collected time-domain spectral data to eliminate high-frequency noise; main waves of the time domain signals are reserved, the echo position is estimated, sampling points are randomly generated through normal distribution to dynamically correct the echo signals, and time domain interference is reduced; and performing fast Fourier transform on the corrected time domain signal, and calculating the refractive index, the absorption coefficient and the dielectric constant of the wheat stripe rust uredospores through frequency domain amplitude and phase information on the basis of an improved Dorne-Duvillaret model. Through sample preparation optimization, echo correction and model improvement, the measurement precision can be remarkably improved.

The invention belongs to the field of biological agriculture, and particularly relates to application of a wheat stripe rust effect protein gene HASP 38 in improvement of stripe rust resistance of wheat. The amino acid sequence of the wheat stripe rust effect protein HASP 38 provided by the invention is as shown in SEQ ID NO: 1, the nucleotide sequence of the wheat stripe rust effect protein gene HASP 38 is as shown in SEQ ID NO: 2, and an RNA interference vector of the gene is provided. According to the invention, an RNA (Ribonucleic Acid) interference vector of the gene is transformed into a Fielder wild type material, so that the HASP 38 gene silencing wheat with resistance to stripe rust CYR32 is obtained. According to the invention, the wheat stripe rust effect protein gene HASP 38 is determined to play a negative regulation role in wheat stripe rust resistance, and a stripe rust resistant wheat material is created by using the negative regulation factor, so that a new gene and germplasm resource are provided for wheat stripe rust resistant breeding.

The invention relates to the technical field of molecular breeding and phytopathology, in particular to a KASP molecular marker of a wheat stripe rust resistant gene and application of the KASP molecular marker. The invention particularly relates to a functional SNP (Single Nucleotide Polymorphism) marker of a wheat stripe rust resistance major gene Chr6B excavated on the basis of Meta-GWAS (Complete Genome Association Analysis), and application of a KASP (Kinase Amplified Sequence Protein) molecular marker system developed on the basis of an SNP locus in wheat disease-resistant germplasm resource screening, molecular marker-assisted breeding and gene pyramiding breeding. The major effect of the MQTL6B.2 site on the Chr6B chromosome in resistance phenotypic variation is disclosed for the first time, and the contribution rate is greater than or equal to 28.54%. The primer can distinguish a disease-resistant material from a susceptible material, and provides an effective molecular marking tool for subsequent disease-resistant breeding. A molecular marker detection tool can be provided for identification of stripe rust-resistant germplasm resources and breeding of disease-resistant varieties, the selection efficiency is improved, and the breeding process is accelerated.

The invention belongs to the technical field of gene engineering, and relates to application of wheat receptor kinase TaSRK1 in improvement of wheat disease resistance. The wheat receptor kinase gene TaSRK1 is analyzed and screened by using a reverse genetics method, it is proved that TaSRK1 is induced to express by stripe rust germs, a CRISPR-Cas9 gene editing technology is adopted to knock out TaSRK1 to create a function deletion mutant, the resistance to main epidemic microspecies of wheat stripe rust is improved, and the resistance to the main epidemic microspecies of wheat stripe rust is improved. It is determined that the wheat receptor kinase gene TaSRK1 plays a negative regulation role in wheat stripe rust resistance, and the wheat stripe rust resistance gene TaSRK1 can be used for wheat stripe rust resistance genetic improvement.

The invention discloses a wheat stripe rust molecular marker and application thereof in stripe rust resistance identification. The wheat stripe rust molecular marker disclosed by the invention is nucleotides corresponding to 29th to 32th sites of SEQ ID No.2 in a wheat genome, and the nucleotides are 29th to 32th sites of SEQ ID No.2 or 29th to 32th sites of SEQ ID No.2 deleted. Experiments prove that wheat materials with stripe rust resistance can be quickly screened out by applying the molecular marker disclosed by the invention, so that the breeding pace of new wheat varieties is accelerated. The method has important theoretical significance and economic value.

The invention belongs to the technical field of gene engineering, and discloses application of a transcription factor TaNAC67 in regulation and control of wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown as SEQ ID NO: 1, and the transcription factor can induce cell necrosis and exert a positive regulation function in the interaction process of wheat and puccinia striiformis. The coding gene TaNAC67 of the transcription factor is a disease-resistant related gene, is infected by stripe rust in non-affinity interaction of plants and stripe rust and is strongly induced to express, and the nucleotide sequence is shown as SEQ ID NO: 2. On the basis of the characteristics, a transgenic plant over-expressing the TaNAC67 gene is further obtained, and it is verified that the transgenic plant shows resistance to stripe rust. The invention provides gene resources and technical support for subsequent genetic improvement of stripe rust resistant wheat materials.

The invention discloses a KASP molecular marker related to wheat stripe rust resistance characters and application of the KASP molecular marker, and belongs to the technical field of molecular markers and plant genetic breeding. SNP sites QYr.hzau4B of the KASP molecular marker are located at the 452, 424 and 208 basic groups of a wheat reference genome 4B chromosome, C / T mutation exists at the sites, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO. 1. The KASP molecular marker can be used for identifying stripe rust resistant and susceptible varieties or strains of wheat, and a valuable molecular tool is provided for screening stripe rust resistant germplasm resources of wheat, so that the breeding efficiency of improved varieties is improved, and the breeding process is accelerated.

Compositions and methods for enhancing the resistance of wheat and barley plants to wheat stripe rust caused by Puccinia striiformis f. sp. tritici are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of wheat and barley plants to wheat stripe rust comprise introducing a nucleic acid molecule encoding an R gene product into a wheat or barley plant cell. Additionally provided are methods for using the wheat and barley plants in agriculture to limit wheat stripe rust.

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.

The utility model discloses wheat stripe rust collecting and storing equipment which comprises a handle, a collector is fixedly connected to the top of the handle, a collecting tank is detachably connected to the bottom of the collector, a material suction opening is fixedly connected to one end of the collector, and a collecting assembly is arranged on the outer wall of the material suction opening. According to the wheat stripe rust fungus collecting and storing equipment, materials can be sucked in through a material suction opening, in the suction process, wheat leaves can be held by the other hand to be straightened, a brush is attached to the wheat leaves, rust fungi on the surfaces of the wheat leaves can be treated through back-and-forth pulling, in addition, when the brush conducts brushing, the wheat leaves can be conveniently cleaned, and the wheat leaves can be conveniently stored. Under the supporting of the supporting spring, the connecting rod can move back and forth through the connecting plate, through the arrangement of the supporting spring, the flexibility, adaptability and durability of the brush can be enhanced, the spring can ensure that the brush and the surface keep proper contact force for uneven wheat leaves, and therefore the material suction effect is improved.