261 results about "Stripe rust" patented technology

The invention discloses application of a wheat WRKY transcription factor gene TaWRKY1, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the gene TaWRKY1 is shown as SEQ ID NO: 1, and the gene TaWRKY1 is infected by stripe rust to induce expression in interaction of wheat and stripe rust, and plays a negative regulation role in wheat stripe rust resistance. A TaWRKY1 gene editing mutant plant is created by using an agrobacterium tumefaciens-mediated genetic transformation method, the sporulation quantity on wheat leaves of the gene editing mutant is obviously reduced, and the allergic necrosis is increased, which indicates that the edited gene TaWRKY1 can improve the resistance of wheat to stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention provides a wheat early-stage stripe rust detection method and system, and the method comprises the steps: inputting time dimension embedded multi-modal data, pre-training a spectrum reconstruction and time sequence comparison learning model, and mining universal feature modes of wheat stripe rust in different development stages and different spectral bands; and transferring the pre-trained weight to a multi-modal encoder of a multi-modal multi-scale target detection model, extracting interactive fusion features of different scales, inputting the interactive fusion features into a multi-scale decoder, comprehensively utilizing global context information and local detail information, and meanwhile, fully utilizing the features of different scales through a cross attention mechanism so as to obtain a multi-scale multi-scale target detection model. Outputting a stripe rust detection result; finely adjusting parameters of the multi-scale decoder by using data with labels in the early-stage stripe rust data to obtain a trained multi-modal multi-scale target detection model; and performing early stripe rust detection on wheat to be detected by using the trained multi-modal multi-scale target detection model. The kit can be used for detecting the early wheat stripe rust.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention belongs to the field of biological agriculture, and particularly relates to a wheat negative regulatory factor TaEDR2 and application thereof. The amino acid sequence of the wheat negative regulatory factor encoding protein provided by the invention is as shown in SEQ ID NO: 1, and the nucleotide sequence of the wheat negative regulatory factor TaEDR2 is as shown in SEQ ID NO: 2. According to the invention, gene editing is carried out in a Fielder wild type material, and a TaEDR2 gene editing plant which shows resistance to stripe rust CYR32 is obtained. It is determined that the TaEDR2 gene plays a negative regulation role in wheat stripe rust resistance, a stripe rust resistant wheat material is created by using the negative regulation factor, and new gene and germplasm resources are provided for wheat stripe rust resistant breeding.

The invention relates to a linked KASP molecular marker of a wheat stripe rust resistance gene YrBN1 and application of the molecular marker. The KASP molecular marker is XK1D-8; the KASP molecular marker and a wheat stripe rust resistance gene YrBN1 are co-localized on a wheat 1DS chromosome, the nucleotide sequence of XK1D-8 is as shown in SEQ ID NO.1, the 100th basic group from the 5'end of the nucleotide sequence is an SNP site, and the polymorphism of the SNP site is C / A. The KASP molecular marker is closely linked with the stripe rust resistant gene YrBN1, can accurately identify wheat stripe rust resistant plants, and is of great significance to wheat germplasm resource stripe rust resistant gene screening, wheat stripe rust resistant material creation or molecular assisted breeding.

The invention provides a wheat stripe rust early warning method, system and device, a storage medium and a program product, and relates to the technical field of agriculture, and the method comprises the steps: obtaining the spore capture amount, meteorological data and field morbidity of a to-be-monitored farmland; the to-be-monitored farmland is used for planting to-be-monitored wheat, the spore capture amount is the capture number of puccinia striiformis wheat specialized spores, and the field morbidity is the probability that the to-be-monitored wheat is infected with wheat stripe rust; based on the meteorological data, the spore capture amount and the field morbidity, constructing a wheat stripe rust prediction model; and based on the wheat stripe rust prediction model, performing wheat stripe rust monitoring and early warning on the to-be-monitored farmland. By means of the mode, efficient, accurate and real-time monitoring and early warning can be conducted on the wheat stripe rust without depending on manual investigation, the accuracy rate is high, the efficiency is high, and outbreak of the wheat stripe rust can be effectively prevented.

The invention belongs to the technical field of genetic engineering, and discloses a phytosusceptibility-related TaCNGC13 gene, a protein and application of the phytosusceptibility-related TaCNGC13 gene and the protein. The phytosusceptible related TaCNGC13 gene is derived from a wheat variety water source 11, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the gene encoding protein is shown as SEQ ID NO: 2. The plant-susceptibility-related TaCNGC13 gene is induced to express by the puccinia striiformis, the expression of the plant-susceptibility-related TaCNGC13 gene in wheat is stably silenced, and the resistance of the obtained transgenic wheat to a plurality of physiological races of the puccinia striiformis is higher than that of wild wheat. The invention provides a novel germplasm material for green control of wheat stripe rust and cultivation of wheat stripe rust resistant varieties.

The invention relates to a KASP molecular marker linked with a wheat stripe rust resistance gene Yr85 and application of the KASP molecular marker. The KASP molecular marker comprises XK1B-63 and / or XK1B-65, the KASP molecular marker and a wheat stripe rust resistance gene Yr85 are co-localized on a wheat 1BS chromosome, the nucleotide sequence of XK1B-63 is as shown in SEQ ID NO.1, and the polymorphism of XK1B-63 is C / G; the nucleotide sequence of the XK1B-65 is as shown in SEQ ID NO. 2, and the polymorphism of the XK1B-65 is A / G. The KASP molecular marker is closely linked with the stripe rust resistant gene Yr85, can accurately identify wheat stripe rust resistant plants, and is of great significance to wheat germplasm resource stripe rust resistant gene screening, wheat stripe rust resistant material creation or molecular assisted breeding.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat magnesium transporter TaNIPA8-6B and application of a coding gene thereof to regulation and control of plant stripe rust resistance. Functional analysis is carried out on the wheat magnesium transporter TaNIPA8-6B by means of gene editing and overexpression, and it is found that the wheat magnesium transporter TaNIPA8-6B induces expression in both affinity and non-affinity system reactions of wheat and puccinia striiformis, so that a mutant plant of the gene editing and overexpression TaNIPA8-6B gene is created; according to the TaNIPA8-6B gene, the gene knockout mutant of the TaNIPA8-6B gene is identified to show susceptibility to stripe rust non-compatible races CYR23, the over-expression mutant plant shows disease resistance to stripe rust compatible races CYR32, the fact that the TaNIPA8-6B gene plays a positive regulation role in the disease resistance of wheat to stripe rust is clear, and a high-quality germplasm resource is provided for stripe rust resistance of wheat.

The invention provides wheat receptor cytoplasm kinase TaRIPK8 and application thereof in improvement of stripe rust resistant varieties of cereal crops, and belongs to the technical field of biological agriculture. The invention provides application of wheat receptor cytoplasm kinase TaRIPK8 in regulation and control of stripe rust resistance of cereal crops and / or improvement or cultivation of stripe rust resistance varieties of the cereal crops. The wheat receptor cytoplasm kinase TaRIPK8 comprises protein with an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The invention further provides application of the wheat receptor cytoplasm kinase TaRIPK8 in regulation and control of the stripe rust resistance character of the cereal crops and / or improvement or cultivation of the stripe rust resistance varieties of the cereal crops. The receptor-like cytoplasm kinase TaRIPK8 participates in negative regulation of wheat on stripe rust, so that a high stripe rust resistance strain can be created on the basis, and an excellent wheat material is provided for cultivation of stripe rust resistance varieties.

The invention provides a molecular marker linked with a wheat stripe rust resistance gene YrZ41-2AL and application of the molecular marker, and belongs to the technical field of molecular markers. The nucleotide sequence of the molecular marker KASP-2AL-2 provided by the invention is as shown in SEQ ID NO. 1, and the polymorphism of the nineteenth base of the nucleotide sequence is T or C. The molecular marker KASP-2AL-2 has the advantages that the nucleotide sequence is shown in SEQ ID NO. The molecular marker KASP-2AL-2 is closely linked with the stripe rust resistant gene YrZ41-2AL on the wheat 2A chromosome, can rapidly and accurately detect the stripe rust resistant gene, and has high application value in the aspects of screening or identifying the wheat stripe rust resistance, cultivating wheat with stripe rust resistance, improving wheat germplasm resources and the like.

The invention belongs to the technical field of gene engineering, and discloses a wheat susceptibility related gene, a protein and application. The wheat susceptibility related gene is a TaBRG10 gene and is derived from a wheat variety water source 11, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the TaBRG10 gene is as shown in SEQ ID NO: 2. The TaBRG10 gene is induced to express by stripe rust, and under the infection of stripe rust, the infection surface of a TaBRG10 gene silent plant infected by stripe rust is positively and obviously lower than that of a control plant, which indicates that the silent TaBRG10 gene improves the resistance of the plant to wheat stripe rust. The invention provides a novel germplasm material for the cultivation of the stripe rust resistant wheat variety.

The invention belongs to the technical field of genetic engineering and molecular breeding, and particularly relates to application of TaHPPD protein in regulation and control of wheat stripe rust resistance. The TaHPPD protein disclosed by the invention can be used for weakening the accumulation of reactive oxygen species (ROS) and cell necrosis reaction caused by CNL protein NLRMro and reducing ion leakage. A TaHPPD gene in wheat is knocked out through a gene editing technology, a TaHPPD knockout (TaHPPD-KO) wheat material is obtained, and wheat with wheat stripe rust resistance is obtained. The result of the embodiment shows that the TaHPPD-KO wheat plant shows obvious resistance compared with the control Fielder. The TaHPPD protein can be used for regulating and controlling the stripe rust resistance of wheat, and can be used for cultivating stripe rust resistant wheat.

The invention belongs to the technical field of biotechnology, and particularly relates to a wheat thermo-sensitive stripe rust resistant gene TaEDR2-7B, a recombinant vector, application and a method. The nucleotide sequence of the gene TaEDR2-7B is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention provides the gene TaEDR-7B related to the temperature-sensitive stripe rust resistance of the wheat as well as the encoding protein and the application of the gene TaEDR-7B for the first time. The invention discloses that the gene TaEDR2-7B plays an important role in the temperature-sensitive disease resistance of the wheat.

The invention relates to the technical field of intelligent agricultural remote sensing monitoring, in particular to a wheat stripe rust multi-scale detection system and method based on unmanned aerial vehicle multi-spectral images. Comprising an attitude compensation image registration module, a sheath and leaf boundary enhancement module, a stripe rust incubation period stripe extraction module, a canopy stomatal density inversion and mapping module and a three-dimensional space infection simulation module. Wherein the attitude compensation image registration module is used for eliminating flight attitude interference; the leaf sheath and leaf junction enhancing module is used for enhancing the structural boundary characteristics of a leaf sheath and leaf junction area; the stripe rust incubation period stripe extraction module is used for extracting disease incubation period stripe features; and the canopy stomatal density inversion and mapping module is used for inverting canopy stomatal conductance parameters. According to the method, by fusing multispectral image feature extraction, thermal infrared stomatal conductance inversion and three-dimensional space propagation modeling, multi-scale accurate detection and visual expression of the wheat stripe rust from incubation period identification to a spatial diffusion process are realized.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention provides a stripe rust resistant gene and application thereof. The stripe rust resistant gene is over-expressed in a material Yannong 19 with high stripe rust susceptibility, and a transgenic positive plant shows near immunity.

The invention discloses a wheat stripe rust resistance regulation gene TaWAK2 as well as an encoding protein and application thereof, and belongs to the technical field of molecular biology. The wheat stripe rust resistance regulation gene TaWAK2 disclosed by the invention is as shown in a sequence table SEQ ID NO: 1, and the length of the wheat stripe rust resistance regulation gene TaWAK2 is 7042bp; the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 4 in a sequence table, and the length of the amino acid sequence is 693. Through a VIGS silencing technology and gene sequencing analysis, it is proved that the gene TaWAK2 plays an important role in wheat stripe rust resistance regulation and control, and the wheat stripe rust resistance is negatively regulated and controlled. The gene TaWAK2 disclosed by the invention can be applied to wheat stripe rust resistance breeding; research on the gene is beneficial to revealing a molecular genetic basis of wheat stripe rust resistance, and an important gene target is provided for wheat breeding for disease resistance.

The invention relates to a KASP molecular marker linked with a wheat stripe rust resistance gene YrZT97 and application of the KASP molecular marker. The KASP molecular marker is XK7B-034; the KASP molecular marker and a wheat stripe rust resistance gene YrZT97 are co-localized on a wheat 7BL chromosome, the nucleotide sequence of XK7B-034 is as shown in SEQ ID NO.1, the 18th basic group from the 5'end of the nucleotide sequence is an SNP site, and the polymorphism of the SNP site is G / A. The KASP molecular marker provided by the invention is closely linked with the stripe rust resistant gene YrZT97, can accurately identify wheat stripe rust resistant plants, and is of great significance to wheat germplasm resource stripe rust resistant gene screening, wheat stripe rust resistant material creation or molecular assisted breeding.

The invention relates to the technical field of molecular genetics, and particularly discloses a Yr6 gene, protein, a recombinant vector and application of the Yr6 gene, the protein and the recombinant vector, the full-length sequence of the Yr6 gene is shown as SEQ ID NO.1, and the CDS sequence of the Yr6 gene is shown as SEQ ID NO.2. Yr6 is overexpressed in a transgenic mode to improve wheat stripe rust resistance. The Yr6 gene provided by the invention is beneficial to improving the resistance of wheat to stripe rust, and provides support for breeding of stripe-rust-resistant wheat.

The invention belongs to the technical field of biology, and particularly relates to a TaGAT2-1B gene resistant to wheat stripe rust and application of the TaGAT2-1B gene, and the nucleotide sequence of the TaGAT2-1B gene is shown as SEQ ID NO.3. It is found that the TaGAT2-1B gene and the encoding protein thereof can effectively improve the wheat stripe rust resistance, the TaGAT2-1B gene is a new stripe rust resistance gene in the wheat adult-plant stage, infection of various stripe rust strains can be effectively resisted, the field phenotype of the TaGAT2-1B gene shows the characteristic of slow rust, and the TaGAT2-1B gene and the encoding protein thereof have good application prospects. The discovery of the gene provides a new genetic resource for the research of the wheat stripe rust resistance mechanism and the wheat stripe rust resistance breeding.

The invention discloses a wheat stripe rust effect protein gene Pst4593 and application thereof, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the wheat stripe rust effect protein gene Pst4593 is shown as SEQ ID NO: 1, the gene positively regulates pathogenicity of stripe rust to wheat, silence of the wheat stripe rust effect protein gene Pst4593 can improve resistance of wheat to stripe rust, and relative expression quantities of wheat related defense genes TaPR1, TaPR2 and TaPR5 are remarkably improved. The wheat stripe rust effect protein gene Pst4593 is related to induced expression of stripe rust aspirator, and the protein coded by the wheat stripe rust effect protein gene Pst4593 also has lipase activity and can influence pathogenicity of stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention belongs to the technical field of bioengineering, and relates to a wheat secretion carrier related membrane protein TaSCAMP6 and application thereof. The amino acid sequence of the wheat secretion vector related membrane protein TaSCAMP6 provided by the invention is as shown in SEQ ID NO: 2; the nucleotide sequence for coding the wheat secretion vector related membrane protein TaSCAMP6 gene is as shown in SEQ ID NO: 1; according to the invention, the function of the TaSCAMP6 gene is researched through a genetic means, transgenosis is carried out in a Fielder wild type material, a TaSCAMP6 overexpression plant is obtained, and verification shows that the resistance of TaSCAMP6 overexpression to stripe rust is enhanced, the gene is determined to play a positive regulation role in stripe rust resistance of wheat, and a new gene resource is provided for stripe rust resistance breeding of wheat.

A broad-spectrum disease resistance-related protein derived from wheat and related biomaterials and use thereof are provided. The protein is selected from the group consisting of A1) a protein with the amino acid sequence of SEQ ID NO: 1; A2) a protein having more than 80% identity to, having a same function as the protein with the amino acid sequence of SEQ ID NO: 1, and obtained by substitutions and / or deletions and / or additions of amino acid residues thereof; and A3) a fusion protein with a same function obtained by joining a tag at N-terminus and / or C-terminus of A1) or A2). The TaCNGC5.1 overexpression lines showed significantly improved disease resistance, thus suggesting that TaCNGC5.1 may play an important role in breeding plants with improved resistance to stripe rust.

The invention provides application of wheat cysteine receptor kinase TaCRK25-1B and a coding gene thereof in regulation and control of stripe rust resistance of plants, and belongs to the technical field of plant genetic engineering. The TaCRK25-1B provided by the invention is subjected to induced expression of stripe rust infection in wheat, a VIGS technology instantaneous silencing experiment shows that the resistance of the wheat to stripe rust CYR31 is enhanced by silencing the TaCRK25-1B, the TaCRK25-1B is over-expressed in the wheat by a genetic transformation technology, the resistance of the wheat to stripe rust is weakened, and an RNAi interference plant infection pathogen experiment of the TaCRK25-1B created by an RNAi technology shows that the TaCRK25-1B has the advantages that the TaCRK25-1B can be used for expressing the stripe rust CYR31 in the wheat; the TACRK25-1B plays a negative regulation role in wheat stripe rust resistance. Therefore, the invention provides a new means for wheat stripe rust resistance and resistant wheat breeding.

The invention belongs to but not limited to the technical field of plant protection, and particularly relates to application and method for improving wheat stripe rust resistance through allogenic material treatment, 250 micromoles of luteolin is used for culturing stripe rust fungus spores, germination of the stripe rust fungus spores is remarkably inhibited, 500 micromoles of luteolin is used for pretreating wheat leaves, spraying is carried out once a day, spraying is carried out three times in total, and the yield of the wheat stripe rust resistance is increased. After the leaves absorb the luteolin, the puccinia striiformis is inoculated, and the result shows that the endogenous luteolin content of the wheat plants treated by the luteolin is increased, the infection area of the puccinia striiformis is remarkably reduced, the number of puccinia striiformis spores on the leaves is remarkably reduced, and the biomass of the puccinia striiformis is remarkably reduced. The invention proves that the occurrence of wheat stripe rust can be inhibited by exogenous luteolin treatment, and a certain reference is provided for stripe rust disease-resistant mechanism research and prevention and control measures.

The invention discloses application of a wheat gene TaALD1 in improving wheat stripe rust resistance, relates to the technical field of biology, and has the technical key points that the nucleotide sequence of the TaALD1 is shown as SEQ ID No.1. According to the invention, TaALD1 is subjected to up-regulation expression after being infected by stripe rust, and virus-induced gene silencing is adopted to determine that TaALD1 plays a positive regulation role in wheat stripe rust resistance; and meanwhile, the transgenic ALD1 plant can obviously improve the stripe rust resistance of the wheat, has a better application prospect in the cultivation of disease-resistant wheat varieties, and lays an important foundation for the disease-resistant breeding of the wheat.

The invention relates to the field of biomolecular detection, in particular to a KASP molecular marker related to wheat stripe rust resistance characters, a primer pair and application of the KASP molecular marker and the primer pair. According to the invention, 559 parts of common wheat germplasm resources are subjected to whole genome association analysis, a stable stripe rust resistance site QYr.hzau-3B. 1 is identified on a wheat 3B chromosome long arm, and a KASP molecular marker (KASP molecular marker KASP 3B2800) is developed aiming at the site and is used for screening large-scale materials. The KASP molecular marker provided by the invention provides a new site and a new marker for wheat stripe rust resistance breeding; meanwhile, the KASP molecular marker can be used for rapidly screening wheat germplasm containing the site, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The invention discloses a terahertz time-domain spectroscopy-based wheat stripe rust uredospore optical parameter extraction method. The method comprises the following steps: mixing wheat stripe rust uredospore powder and polyethylene powder, and tabletting; a transmission module of the terahertz time-domain spectrometer is used for collecting time-domain spectral data of the mixed tablets in a nitrogen environment; carrying out filtering processing on the collected time-domain spectral data to eliminate high-frequency noise; main waves of the time domain signals are reserved, the echo position is estimated, sampling points are randomly generated through normal distribution to dynamically correct the echo signals, and time domain interference is reduced; and performing fast Fourier transform on the corrected time domain signal, and calculating the refractive index, the absorption coefficient and the dielectric constant of the wheat stripe rust uredospores through frequency domain amplitude and phase information on the basis of an improved Dorne-Duvillaret model. Through sample preparation optimization, echo correction and model improvement, the measurement precision can be remarkably improved.