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136 results about "Wheat diseases" patented technology

The cereal grain wheat is subject to numerous wheat diseases, including bacterial, viral and fungal diseases, as well as parasitic infestations.

Tilletia controversa Kuhn effect protein g16561 and application thereof

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.
Owner:SANYA NATIONAL INSTITUTE OF SOUTHERN BREEDING CHINESE ACADEMY OF AGRICULTURAL SCIENCES +2

Lightweight detection method for wheat diseases and insect pests in northwest weather based on YOLOv8 improvement

The invention discloses a YOLOv8 improvement-based lightweight detection method for wheat diseases and insect pests in northwest weather, and relates to the technical field of artificial intelligence and machine vision monitoring. The method comprises the following steps: acquiring an original RGB image in a wheat filling period in a northwest dry land area environment; performing screening and Mosaic-8 data enhancement on the collected original image data set, and establishing a wheat disease and insect pest image data set; constructing an improved wheat lesion detection lightweight model based on YOLOv8, and optimizing a network structure; inputting the established wheat disease and insect pest image data set into the improved network model for training; testing the application of the improved network model in a wheat disease and insect pest image test data set, and continuously updating learning parameters of the improved YOLOv8 network model; the updated and trained YOLOv8 network model is used for monitoring a dry land area where wheat is planted in the Gansu province in real time, and the complexity and accuracy of the model are evaluated; and finally, real-time feedback of a model detection result is realized, and wheat plant diseases are dynamically updated to improve model precision.
Owner:LANZHOU JIAOTONG UNIV

Wheat susceptibility related gene, protein and application

The invention belongs to the technical field of gene engineering, and discloses a wheat susceptibility related gene, a protein and application. The wheat susceptibility related gene is a TaBRG10 gene and is derived from a wheat variety water source 11, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the TaBRG10 gene is as shown in SEQ ID NO: 2. The TaBRG10 gene is induced to express by stripe rust, and under the infection of stripe rust, the infection surface of a TaBRG10 gene silent plant infected by stripe rust is positively and obviously lower than that of a control plant, which indicates that the silent TaBRG10 gene improves the resistance of the plant to wheat stripe rust. The invention provides a novel germplasm material for the cultivation of the stripe rust resistant wheat variety.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

Application of wheat transcription factor TaWRKY22 and coding gene thereof in regulation and control of plant stripe rust resistance

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

Application of Fpg13 gene as target in prevention and treatment of wheat diseases caused by fusarium pseudograminearum

The invention discloses application of an Fpg13 gene as a target in prevention and treatment of wheat diseases caused by fusarium pseudograminearum, and belongs to the technical field of biology. The invention researches and verifies that the Fpg13 protein is a fusarium pseudograminearum key toxic effect protein for the first time, and plays an important role in the process of infecting wheat by the fusarium pseudograminearum. By means of a PEG-mediated homologous recombination knockout technology, the effect protein Fpg13 gene of the fusarium pseudograminearum is knocked out, and the pathogenic ability of the fusarium pseudograminearum to wheat is weakened by the knockout mutant after knockout. The invention lays a theoretical foundation for developing the fusarium pseudograminearum small-molecule inhibitor by utilizing pathogenic factors of pathogenic bacteria, and has important significance for developing novel drugs of the pathogenic bacteria and inhibiting the generation of drug resistance.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Wheat disease intelligent diagnosis method and system based on multi-modal large model

The invention discloses an intelligent wheat disease diagnosis method and system based on a multi-modal large model. The method comprises the following steps: constructing a knowledge graph; obtaining a geographic position and current time of a user, positioning a wheat planting area based on the geographic position, calculating a wheat growth period in combination with the current time and the wheat planting area, and selecting a corresponding knowledge graph sub-graph according to the wheat growth period and the planting area; collecting texts and pictures input by a user through guiding type interaction; extracting text keywords, and identifying disease sites, disease types and disease symptoms in the pictures by using a deep learning model; fusing the text analysis result and the image analysis result to form a multi-modal feature; the optimized features are screened from the multi-modal features; a keyword set is decoded from the optimized features; generating a query statement based on the keyword set; and retrieving answers from the knowledge graph sub-graphs based on the query statements, and generating response contents based on the answers and feeding back the response contents to the user. The accuracy and efficiency of wheat disease diagnosis are improved.
Owner:NANJING INST OF TECH +1

Cloning of wheat broad-spectrum powdery mildew-resistant gene pm26 and use thereof

PCT designated stage expiredWO2025103148A1Plant peptidesFermentationBiotechnologyGermplasm
Cloning of a wheat broad-spectrum powdery mildew-resistant gene Pm26 and a use thereof. A Pm26 protein is a protein composed of the amino acid sequence represented by SEQ ID No. 3. Also provided is a gene Pm26, wherein a coding region is DNA molecules represented by positions 3001-9489 in SEQ ID No. 1, or the coding region is DNA molecules represented by SEQ ID No. 2. Further provided are gene mapping, map-based cloning and powdery mildew-resistant biological function identification methods for the wheat broad-spectrum powdery mildew-resistant gene PM26. The wheat broad-spectrum powdery mildew-resistant gene Pm26 can be widely used in plant fields of wheat disease-resistant genetic breeding, germplasm resource improvement, transgenic and genome editing breeding and the like, and plays an important role in improving and enhancing the germplasm resources of crops such as wheat.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

KASP molecular marker of wheat stripe rust resistant gene and application of KASP molecular marker

The invention relates to the technical field of molecular breeding and phytopathology, in particular to a KASP molecular marker of a wheat stripe rust resistant gene and application of the KASP molecular marker. The invention particularly relates to a functional SNP (Single Nucleotide Polymorphism) marker of a wheat stripe rust resistance major gene Chr6B excavated on the basis of Meta-GWAS (Complete Genome Association Analysis), and application of a KASP (Kinase Amplified Sequence Protein) molecular marker system developed on the basis of an SNP locus in wheat disease-resistant germplasm resource screening, molecular marker-assisted breeding and gene pyramiding breeding. The major effect of the MQTL6B.2 site on the Chr6B chromosome in resistance phenotypic variation is disclosed for the first time, and the contribution rate is greater than or equal to 28.54%. The primer can distinguish a disease-resistant material from a susceptible material, and provides an effective molecular marking tool for subsequent disease-resistant breeding. A molecular marker detection tool can be provided for identification of stripe rust-resistant germplasm resources and breeding of disease-resistant varieties, the selection efficiency is improved, and the breeding process is accelerated.
Owner:XINJIANG AGRI UNIV

Application of wheat receptor kinase TaSRK1 in improvement of wheat disease resistance

The invention belongs to the technical field of gene engineering, and relates to application of wheat receptor kinase TaSRK1 in improvement of wheat disease resistance. The wheat receptor kinase gene TaSRK1 is analyzed and screened by using a reverse genetics method, it is proved that TaSRK1 is induced to express by stripe rust germs, a CRISPR-Cas9 gene editing technology is adopted to knock out TaSRK1 to create a function deletion mutant, the resistance to main epidemic microspecies of wheat stripe rust is improved, and the resistance to the main epidemic microspecies of wheat stripe rust is improved. It is determined that the wheat receptor kinase gene TaSRK1 plays a negative regulation role in wheat stripe rust resistance, and the wheat stripe rust resistance gene TaSRK1 can be used for wheat stripe rust resistance genetic improvement.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

Application of wheat disease-resistant factor TaCBL4 in prevention and treatment of basal stem rot

The invention belongs to the technical field of genetic engineering, and relates to application of a wheat disease-resistant factor TaCBL4 in prevention and treatment of basal stem rot. A wheat strain capable of overexpressing the wheat disease-resistant factor gene TaCBL4 is obtained by adopting an agrobacterium-mediated method, and the condition that the wheat disease-resistant factor TaCBL4 plays a positive regulation role in the immune response of wheat stem rot resistance is known by comparing the morbidity condition and the fungal biomass condition of a receptor wheat Fielder infected with stem rot pathogens. On the basis, the wheat disease-resistant factor TaCBL4 provided by the invention can be used for creating a stem rot resistant wheat germplasm material, and plays an important role in prevention and treatment of wheat stem rot.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

Wheat disease identification method and device

The invention provides a wheat disease identification method and device, and is applied to the technical field of crop disease identification. The method comprises the steps of obtaining a training sample set; determining mass-to-charge ratio signal intensity data of volatile organic compounds of the wheat leaf samples in the training sample set, determining a maximum characteristic value of each mass-to-charge ratio channel in the mass-to-charge ratio signal intensity data, mapping the maximum characteristic values of all the mass-to-charge ratio channels into pixel points, and obtaining a two-dimensional pixel matrix of the wheat leaf samples; extracting parent ions and pixel points where fragment ions corresponding to the parent ions are located from the two-dimensional pixel matrix, and rearranging the positions of the extracted pixel points in the two-dimensional pixel matrix to obtain a recombined feature map; and inputting the recombinant feature map into a wheat disease recognition model for network training, wherein the wheat disease recognition model is used for recognizing the disease type and infection degree of wheat according to the mass-to-charge ratio signal features in the recombinant feature map.
Owner:INTELLIGENT EQUIPMENT RESEARCH CENTER BEIJING ACADEMY OF AGRICULTURE AND FORESTRY SCIENCES

Application of transcription factor TaNAC67 in regulation and control of wheat disease resistance

The invention belongs to the technical field of gene engineering, and discloses application of a transcription factor TaNAC67 in regulation and control of wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown as SEQ ID NO: 1, and the transcription factor can induce cell necrosis and exert a positive regulation function in the interaction process of wheat and puccinia striiformis. The coding gene TaNAC67 of the transcription factor is a disease-resistant related gene, is infected by stripe rust in non-affinity interaction of plants and stripe rust and is strongly induced to express, and the nucleotide sequence is shown as SEQ ID NO: 2. On the basis of the characteristics, a transgenic plant over-expressing the TaNAC67 gene is further obtained, and it is verified that the transgenic plant shows resistance to stripe rust. The invention provides gene resources and technical support for subsequent genetic improvement of stripe rust resistant wheat materials.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

Application of wheat gene taald1 in improving resistance to stripe rust in wheat

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.
Owner:GANSU AGRI UNIV

Application of wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.
Owner:HAINAN NORMAL UNIV

Wheat stripe rust adult plant resistance gene Yr54, specific functional molecular marker and application of wheat stripe rust resistance gene Yr54 in wheat stripe rust resistance breeding

The invention discloses a wheat stripe rust adult plant resistance gene Yr54, a specific functional molecular marker and application of the wheat stripe rust adult plant resistance gene Yr54 in wheat stripe rust resistance breeding, and belongs to the technical field of biology. A wheat stripe rust adult-plant resistance gene TaNLR2DL transgenic line is subjected to adult-plant-stage stripe rust resistance identification, and a result shows that compared with a non-TaNLR2DL gene line, the stripe rust resistance of the TaNLR2DL transgenic line is obviously improved, while a TaNLR2DL gene knockout line is contrary to the TaNLR2DL gene knockout line, so that the TaNLR2DL is a potential stripe rust resistance gene, and the TaNLR2DL gene knockout line can be used for identifying the stripe rust resistance in the adult-plant stage. The method can be used for genetic improvement of wheat stripe rust resistance. The invention further develops a functional specific molecular marker of TaNLR2DL, and the marker can quickly screen wheat germplasm containing TaNLR2DL, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.
Owner:HUAZHONG AGRI UNIV

A wheat leaf rust resistance linkage SNP site, a dCAPS molecular marker, a primer, a kit and an application thereof

The application discloses a wheat leaf rust resistance linkage SNP site, a dCAPS molecular marker, a primer, a kit and application, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The application provides a wheat leaf rust resistance linkage SNP site, wherein the SNP site is located at the 134261400th base on the 4D chromosome of a Chinese spring reference sequence with the version number of IWGSC_v1.1, and the polymorphism site is T / G. With the aid of 277 wheat materials introduced from Turkey with genotype data, and in combination with the Chinese spring reference sequence, a dCAPS molecular marker of the SNP site linked to the wheat adult stage leaf rust resistance genetic locus is developed, different wheat can be accurately and rapidly detected, early molecular assisted selection of the adult stage leaf rust resistance germplasm material is realized, and the efficiency of wheat disease resistance breeding is improved.
Owner:HEBEI AGRICULTURAL UNIV.

Wheat powdery mildew resistance related MlIW92 protein and related biological material and application thereof

The invention discloses MlIW92 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to improve the resistance of wheat to powdery mildew. The MlIW92 protein disclosed by the invention is a protein of A1, A2 or A3 as follows: A1 is a protein of which the amino acid sequence is an amino acid sequence shown as SEQ ID No.2 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence as shown in SEQ ID No.2 in the sequence table, has 80% or more of identity with the protein as shown in A1, and is related to wheat powdery mildew resistance; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2. The protein disclosed by the invention is derived from wild two-grain wheat, and powdery mildew resistance gene resources of wheat disease resistance breeding work are enriched.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

Application of wheat disease-resistant factor TaPKS26 in prevention and treatment of brown leaf rust

ActiveCN120944944APlant peptidesFermentationBiotechnologyGenetically modified wheat
The invention belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease-resistant factor TaPKS26 in prevention and treatment of leaf rust. The CDS sequence of the encoding gene of the wheat disease-resistant factor TaPKS26 is shown as SEQ ID NO: 1, a transgenic wheat strain of the overexpressed gene TaPKS26 is obtained by adopting an agrobacterium-mediated method, and the transgenic wheat strain of the overexpressed gene TaPKS26 is proved to have enhanced resistance to wheat leaf rust compared with Fielder wheat after being infected with physiological races THT and PHT, and the wheat leaf rust resistance is improved. The expression quantity of the disease-resistant related marker gene is up-regulated, which indicates that the wheat disease-resistant factor TaPKS26 plays a positive regulation role in wheat leaf rust-resistant defensive reaction. The invention provides new gene resources and technical thoughts for the cultivation of leaf rust resistant varieties.
Owner:NORTHWEST A & F UNIV

Molecular marker for detecting stripe rust and brown leaf rust resistant one-cause multiple-effect sites of wheat and use method of molecular marker for detecting stripe rust and brown leaf rust resistant one-cause multiple-effect sites of wheat

The invention discloses a molecular marker for detecting stripe rust and brown leaf rust resistant one-cause multiple-effect sites of wheat and a use method, and belongs to the technical field of nucleotide polymorphism and molecular markers. The technical problem to be solved by the invention is how to quickly and accurately screen or assist in screening wheat with stripe rust or / and leaf rust resistance or / and breed or assist in breeding wheat single plants with stripe rust or / and leaf rust resistance. The invention provides a composition for detecting single nucleotide polymorphism or genotype of a specific SNP site in a wheat genome, the SNP site can be the 47th nucleotide of a DNA fragment as shown in SEQ ID NO: 1 in the wheat genome, and the type of the nucleotide can be A or T. The primer composition and the molecular marker provided by the invention assist in wheat breeding for resisting stripe rust and leaf rust in wheat disease resistance breeding, greatly shorten the breeding period and play an important role in wheat breeding.
Owner:ZHONGYUAN RES CENT +1

Application of wheat disease-resistant factor TaCIPK10 in prevention and treatment of basal stem rot

The invention belongs to the technical field of genetic engineering, and relates to application of a wheat disease-resistant factor TaCIPK10 in prevention and treatment of basal stem rot. A wheat strain capable of overexpressing the wheat disease-resistant factor gene TaCIPK10 is obtained by adopting an agrobacterium-mediated method, and the condition that the wheat disease-resistant factor gene TaCIPK10 plays a positive regulation role in the immune response of wheat stem rot resistance is known by comparing the morbidity condition and the fungal biomass condition of a receptor wheat Fielder infected with stem rot pathogens. On the basis, the wheat disease-resistant factor TaCIPK10 provided by the invention can be used for creating a stem rot resistant wheat germplasm material, and plays an important role in prevention and treatment of wheat stem rot.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

KASP molecular marker for detecting stripe rust resistance gene of wheat and application of KASP molecular marker

The invention belongs to the technical field of molecular biology, and relates to a KASP molecular marker for wheat stripe rust resistance gene detection and application. The KASP molecular marker XK6B-5 disclosed by the invention is obtained by utilizing a specific primer group as shown in SEQ ID NO.2-4 for amplification; the KASP molecular marker XK6B-5 is closely linked with a wheat stripe rust resistance gene YrZT94.1, and has a co-separation characteristic, and the polymorphism of the XK6B-5 is G / T. The KASP molecular marker provided by the invention can accurately track the stripe rust resistance gene YrZT94.1 of wheat, performs molecular marker-assisted selection, and improves the breeding efficiency of wheat disease resistance. The method can be applied to screening of the stripe rust resistant gene YrZT94.1 in wheat germplasm resources in a simple, rapid and high-throughput manner, so that the method is applied to molecular design breeding, and the wheat breeding process is greatly accelerated.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

KASP molecular marker for detecting stripe rust resistance gene YrZT94.2 of wheat and application of KASP molecular marker

The invention belongs to the technical field of molecular biology, and relates to a KASP molecular marker for detecting a wheat stripe rust resistance gene YrZT94.2 and application of the KASP molecular marker. The KASP molecular marker XK7B-32 disclosed by the invention is obtained by utilizing a specific primer group as shown in SEQ ID NO.2-4 for amplification; the KASP molecular marker XK7B-32 is closely linked with a wheat stripe rust resistance gene YrZT94.2, and has a co-separation characteristic, and the polymorphism of the XK7B-32 is C / G. The KASP molecular marker provided by the invention can accurately track the stripe rust resistance gene YrZT94.2 of wheat, performs molecular marker-assisted selection, and improves the breeding efficiency of wheat disease resistance. The method can be simply and quickly applied to screening of the stripe rust resistant gene YrZT94.2 in wheat germplasm resources with high throughput, so that the method is applied to molecular design breeding, and the wheat breeding process is greatly accelerated.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

Use of a wheat receptor kinase, ta spr12, to improve disease resistance in wheat

This invention belongs to the field of genetic engineering technology and relates to the application of wheat receptor kinase TaSPR12 in improving wheat disease resistance. This invention utilizes reverse genetics methods to analyze and screen wheat receptor kinase genes. TaSPR12 This gene is induced to express by the stripe rust pathogen. The wheat receptor kinase gene was knocked out using CRISPR-Cas9 gene editing technology. TaSPR12 The created loss-of-function mutants exhibited enhanced resistance to the main prevalent races of wheat stripe rust. This invention identified the wheat receptor kinase gene. TaSPR12 It plays a negative regulatory role in wheat resistance to stripe rust and can be used for genetic improvement of wheat resistance to rust.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

KASP Molecular Marker Linked to Wheat Stripe Rust Resistance Gene YrG363 and Its Application

The present invention discloses KASP molecular markers linked to the wheat stripe rust resistance gene YrG363 and their applications, belonging to the field of agricultural biotechnology. The KASP molecular markers include Sicau-1BS.1 and Sicau-1BS.2, and their nucleotide sequences are as shown in SEQ ID NO.1-SEQ ID NO.2. The KASP molecular markers can be used for the fine mapping of YrG363 and molecular marker-assisted selection of the YrG363 gene, improving the efficiency of wheat disease-resistant breeding. The present invention overcomes the disadvantages in traditional disease-resistant breeding, such as the susceptibility of stripe rust resistance phenotype selection to environmental influence, poor stability and repeatability, and low efficiency, thus accelerating the breeding process of wheat varieties resistant to stripe rust. The KASP molecular markers do not rely on gel electrophoresis and can achieve high-throughput and large-scale detection of the YrG363 gene locus.
Owner:SICHUAN AGRI UNIV

Method for identifying and breeding stripe rust-resistant germplasm resources of wheat

The invention belongs to the field of genetic engineering, and particularly relates to a wheat stripe rust resistance germplasm resource identification and breeding method. The CDS nucleotide sequence of the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 2. The developed functional molecular marker Yrym112-InDel comprises an upstream primer sequence as shown in SEQ ID NO. 3 and a downstream primer sequence as shown in SEQ ID NO. 4. A material containing the Yrym112 gene can be divided into a disease-resistant genotype and a susceptible genotype according to the size of an amplified band. The molecular marker is used for identifying or breeding wheat stripe rust resistant germplasm resources, or wheat gene Yr5 typing and wheat molecular marker assisted breeding are used, and the breeding process of wheat disease resistant varieties is accelerated.
Owner:FOOD CROPS RES INST YUNNAN ACAD OF AGRI SCI

Application of transcription factor tabhlh168 protein in wheat disease resistance

The application relates to the technical field of genetic engineering, and discloses application of a transcription factor TabHLH168 protein in wheat disease resistance. The amino acid sequence of the TabHLH168 protein is shown as SEQ ID NO. 1; the coding gene sequence of the TabHLH168 protein is shown as SEQ ID NO. 2. The TabHLH168 protein is a target protein of a false smut fungus effect protein FpE02. The application uses the above-mentioned application of the transcription factor TabHLH168 protein in wheat disease resistance, overexpresses the coding gene of the TabHLH168 protein, obtains transgenic wheat with stable heredity and wheat stem base rot resistance, provides a new solution for carrying out wheat disease resistance breeding work, and has important significance in the field of wheat breeding with disease resistance.
Owner:INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI

Cloning and application of wheat broad-spectrum multi-resistance gene WAI-B2

The invention discloses cloning and application of a wheat broad-spectrum multi-resistance gene WAI-B2, and belongs to the technical field of genetic engineering breeding. The technical problem to be solved by the invention is how to improve the stress resistance of plants, for example, how to improve the disease resistance of wheat. In order to solve the technical problems, in the first aspect, the invention provides application of the protein or a substance for regulating gene expression or a substance for regulating the activity or content of the protein in regulation of plant stress resistance, the protein is coded by the gene, and the protein is WAI-B2 protein. The invention provides a biological function identification method for gene positioning, map-based cloning and disease resistance of a wheat stress resistance gene WAI-B2. The wheat stress resistance gene WAI-B2 can be widely applied to the plant fields of wheat disease-resistant genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and plays an important role in improving germplasm resources of crops such as wheat and the like.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

Wheat disease intelligent diagnosis system and method integrating image recognition and expert system

The invention relates to an image recognition and expert system fused wheat disease intelligent diagnosis system and method, and belongs to the technical field of wheat disease diagnosis. Different processing modes are adopted for different detection results; for the condition that the disease image features are detected and the corresponding disease is unique, the disease result is directly output; a second expert system module performs further identification on the condition that the detected disease image features are not unique corresponding to the disease; and for the condition that no disease image feature is detected, the first expert system module performs diagnosis, if the disease type is diagnosed, a disease result is output, and if the disease type is not diagnosed, the manual judgment module can perform further diagnosis. According to the system and the method, all types of scenes such as obvious image features, similar image features, non-image feature dominant and unknown diseases can be covered, and intelligent diagnosis of various types of wheat diseases is realized.
Owner:河南开放大学

WHEAT VOLTAGE-DEPENDENT ANION CHANNEL GENE TaVDAC1 AND USE THEREOF

The present disclosure belongs to the field of genetic engineering technology and relates to a voltage-dependent anion channel gene TaVDAC1 of wheat and use thereof. The wheat voltage-dependent anion channel gene TaVDAC1 negatively regulates wheat resistance to Fusarium head blight (FHB). When the gene TaVDAC1 is overexpressed, the resistance of wheat to FHB decreases; conversely, when the gene TaVDAC1 is silenced or edited, the resistance of wheat to FHB significantly increases. The wheat voltage-dependent anion channel gene TaVDAC1 may be used for the breeding and development of crop variety or germplasm resources with resistance to FHB, thereby enhancing wheat disease resistance and addressing the technical issues of wheat susceptibility to FHB and the reliance on chemical control methods for its management.
Owner:NORTHWEST A & F UNIV

Wheat disease-resistant factor gene TaPsaF and application thereof

The invention belongs to the technical field of genetic engineering breeding, and discloses a wheat disease-resistant factor gene TaPsaF and application thereof. A TaPsaF gene sequence is analyzed, and a TaPsaF-GFP fusion protein is constructed, so that the localization of the TaPsaF-GFP fusion protein in cell chloroplast is determined. An overexpression and silence vector of the TaPsaF gene is successfully constructed by using a gene engineering technology, and wheat is transformed by using an agrobacterium-mediated method to obtain a transgenic wheat material. Functional verification shows that over-expression of the TaPsaF gene can significantly enhance the resistance of wheat to stripe rust, and silencing of the gene leads to reduction of the resistance. Gene resources and a technical path are provided for wheat stripe rust resistance molecular breeding, the blank of the TaPsaF protein in biological stress response mechanism research is filled up, and the TaPsaF protein has important significance for guaranteeing food safety.
Owner:HUAIBEI NORMAL UNIVERSITY