61 results about "Wheat diseases" patented technology

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.

The invention discloses a wheat stripe rust adult plant resistance gene Yr54, a specific functional molecular marker and application of the wheat stripe rust adult plant resistance gene Yr54 in wheat stripe rust resistance breeding, and belongs to the technical field of biology. A wheat stripe rust adult-plant resistance gene TaNLR2DL transgenic line is subjected to adult-plant-stage stripe rust resistance identification, and a result shows that compared with a non-TaNLR2DL gene line, the stripe rust resistance of the TaNLR2DL transgenic line is obviously improved, while a TaNLR2DL gene knockout line is contrary to the TaNLR2DL gene knockout line, so that the TaNLR2DL is a potential stripe rust resistance gene, and the TaNLR2DL gene knockout line can be used for identifying the stripe rust resistance in the adult-plant stage. The method can be used for genetic improvement of wheat stripe rust resistance. The invention further develops a functional specific molecular marker of TaNLR2DL, and the marker can quickly screen wheat germplasm containing TaNLR2DL, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The application discloses a wheat leaf rust resistance linkage SNP site, a dCAPS molecular marker, a primer, a kit and application, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The application provides a wheat leaf rust resistance linkage SNP site, wherein the SNP site is located at the 134261400th base on the 4D chromosome of a Chinese spring reference sequence with the version number of IWGSC_v1.1, and the polymorphism site is T / G. With the aid of 277 wheat materials introduced from Turkey with genotype data, and in combination with the Chinese spring reference sequence, a dCAPS molecular marker of the SNP site linked to the wheat adult stage leaf rust resistance genetic locus is developed, different wheat can be accurately and rapidly detected, early molecular assisted selection of the adult stage leaf rust resistance germplasm material is realized, and the efficiency of wheat disease resistance breeding is improved.

The invention discloses MlIW92 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to improve the resistance of wheat to powdery mildew. The MlIW92 protein disclosed by the invention is a protein of A1, A2 or A3 as follows: A1 is a protein of which the amino acid sequence is an amino acid sequence shown as SEQ ID No.2 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence as shown in SEQ ID No.2 in the sequence table, has 80% or more of identity with the protein as shown in A1, and is related to wheat powdery mildew resistance; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2. The protein disclosed by the invention is derived from wild two-grain wheat, and powdery mildew resistance gene resources of wheat disease resistance breeding work are enriched.

This invention belongs to the field of genetic engineering technology and relates to the application of wheat receptor kinase TaSPR12 in improving wheat disease resistance. This invention utilizes reverse genetics methods to analyze and screen wheat receptor kinase genes. TaSPR12 This gene is induced to express by the stripe rust pathogen. The wheat receptor kinase gene was knocked out using CRISPR-Cas9 gene editing technology. TaSPR12 The created loss-of-function mutants exhibited enhanced resistance to the main prevalent races of wheat stripe rust. This invention identified the wheat receptor kinase gene. TaSPR12 It plays a negative regulatory role in wheat resistance to stripe rust and can be used for genetic improvement of wheat resistance to rust.

The invention belongs to the field of genetic engineering, and particularly relates to a wheat stripe rust resistance germplasm resource identification and breeding method. The CDS nucleotide sequence of the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 2. The developed functional molecular marker Yrym112-InDel comprises an upstream primer sequence as shown in SEQ ID NO. 3 and a downstream primer sequence as shown in SEQ ID NO. 4. A material containing the Yrym112 gene can be divided into a disease-resistant genotype and a susceptible genotype according to the size of an amplified band. The molecular marker is used for identifying or breeding wheat stripe rust resistant germplasm resources, or wheat gene Yr5 typing and wheat molecular marker assisted breeding are used, and the breeding process of wheat disease resistant varieties is accelerated.

The invention relates to an image recognition and expert system fused wheat disease intelligent diagnosis system and method, and belongs to the technical field of wheat disease diagnosis. Different processing modes are adopted for different detection results; for the condition that the disease image features are detected and the corresponding disease is unique, the disease result is directly output; a second expert system module performs further identification on the condition that the detected disease image features are not unique corresponding to the disease; and for the condition that no disease image feature is detected, the first expert system module performs diagnosis, if the disease type is diagnosed, a disease result is output, and if the disease type is not diagnosed, the manual judgment module can perform further diagnosis. According to the system and the method, all types of scenes such as obvious image features, similar image features, non-image feature dominant and unknown diseases can be covered, and intelligent diagnosis of various types of wheat diseases is realized.

The application discloses a wheat disease-resistant gene TaLOX and application thereof in regulating resistance to stripe rust. TaLOX The application overexpresses the wheat gene in wheat, inoculates the overexpressed wheat plants with stripe rust, and observes the resistance phenotype of the overexpressed wheat plants to the stripe rust (Puccinia striiformis f. Puccinia striiformis sp. tritici ). TaLOX The results show that, compared with a control group, the amount of spores of the stripe rust on the surface of the overexpressed wheat plants is significantly reduced, the disease index and the fungal biomass are significantly reduced, and the mycelium length is significantly shorter than that of the control group, indicating that the gene positively regulates the resistance of the wheat to the stripe rust. TaLOX The application has application prospects in breeding of wheat varieties resistant to stripe rust and improvement of the resistance level of the wheat to the stripe rust.

The application discloses a wheat salicylic acid receptor NPR3 gene and application thereof in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The application discloses application of a wheat salicylic acid receptor NPR3 gene or related biological materials thereof, and the application is as follows: A1, improving the resistance of wheat to stripe rust; A2, improving the resistance of wheat to powdery mildew; A3, improving the resistance of wheat to leaf rust; and the nucleotide sequence of the wheat salicylic acid receptor NPR3 gene is shown as SEQ ID NO. 3. The results of the application show that NPR3 gene knockout lines are resistant to different stripe rust physiological races CYR17, CYR32, CYR33 and CYR34, and further resistance identification shows that the NPR3 gene knockout lines are resistant to powdery mildew and leaf rust. The application provides theoretical guidance and genetic gene resources for wheat disease resistance breeding.

The invention discloses a candidate gene AetNLR2CIae8 of a powdery mildew resistant gene pmAeCIae82DS derived from aegilops tauschii as well as a protein coded by the candidate gene AetNLR2CIae8 and application of the candidate gene AetNLR2CIae8, and the nucleotide sequence of the candidate gene AetNLR2CIae8 of the powdery mildew resistant gene derived from the aegilops tauschii is as shown in SEQ ID NO. 1. 1, and amino acid sequences coded by the gene are respectively as shown in SEQ ID NO. 2. The pmAeCIae82DS gene sequence belongs to the protection range of the invention. The invention also protects the application of the candidate gene AetNLR2CIae8 of the pmAeCIae82DS in the improvement of the disease resistance of the plants. The AetNLR2CIae8 is transformed into the susceptible wheat variety Yangmai 158 by utilizing a transient overexpression system, so that the aspirator index of powdery mildew can be obviously reduced; when the VIGS system is used for respectively silencing AetNLR2CIae8 in the aegilops sphaeroides powdery mildew resistant material TX11-1 containing the pmAeCIae82DS, the susceptibility of plants can be improved. Therefore, the AetNLR2CIae8 can positively regulate and control the wheat powdery mildew resistance and is an important gene resource for wheat disease-resistant breeding.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat VOZ transcription factor or a coding gene thereof in regulation and control of stripe rust resistance of crops. The invention systematically reveals that wheat transcription factors TaVOZ1 and TaVOZ2 play a positive regulation role in immune response of wheat to stripe rust for the first time, and enriches a functional spectrum of a VOZ family in plant immune regulation; meanwhile, the broad-spectrum resistance of an overexpression material to wheat stripe rust (Pst) is remarkably enhanced by overexpressing the encoding gene of the wheat VOZ transcription factor in wheat, and the wheat VOZ transcription factor has practical breeding application potential, so that the wheat VOZ transcription factor encoding gene provided by the invention can be used as an important candidate for breeding disease-resistant molecules; a new way and a new strategy are provided for green prevention and control of wheat diseases.

The application discloses a candidate gene NLR-G4 derived from a Timopheevi wheat powdery mildew resistance gene Pm6 and application thereof.The nucleotide sequence of the candidate gene NLR-G4 derived from the Timopheevi wheat powdery mildew resistance gene is shown in SEQ ID NO.1.The application also protects the application of the candidate gene NLR-G4 of Pm6 in improving plant disease resistance.Using a transient overexpression system, the NLR-G4 can be transformed into a susceptible wheat variety Yangmai 158, so that the sinker index can be significantly reduced; using a VIGS system, the NLR-G4 can be silenced in a disease-resistant material IGV1-465, so that the plant disease susceptibility is enhanced.Therefore, the NLR-G4 positively regulates the powdery mildew resistance, and has important significance for wheat disease-resistant breeding.

The invention belongs to the technical field of molecular genetic breeding, and particularly relates to an SNP site for identifying wheat sharp eyespot resistance, a CAPS molecular marker primer and application of the SNP site and the CAPS molecular marker primer. The SNP (Single Nucleotide Polymorphism) sites of the wheat sharp eyespot resistance disclosed by the invention comprise 6A-AX-111665286 and / or 6A-AX-110674425, and the SNP sites of the wheat sharp eyespot resistance disclosed by the invention comprise the The 6A-AX-111665286 is located at the 600397542bp position of a wheat 6AL chromosome, and T / G polymorphism exists in the 6A-AX-111665286. The 6A-AX-110674425 is located at the 600382189th bp position of a wheat 6AL chromosome, and the A / G polymorphism exists in the 6A-AX-110674425. The molecular marker developed by using the SNP site can accurately and rapidly detect the sheath blight resistance of wheat, can be used for early molecular assisted selection of disease-resistant wheat, and improves the breeding efficiency of wheat disease resistance.

This invention discloses the application of the wheat gene TaMIPS in regulating resistance to Fusarium head blight and stem rot. Through the creation of wheat materials, it was demonstrated that overexpression of the TaMIPS gene significantly enhances wheat resistance to Fusarium head blight and stem rot, while mutants exhibit decreased disease resistance. Furthermore, this invention utilizes gene editing technology to optimize the promoter region of the TaMIPS gene, removing the inhibitory effect of the upstream non-coding open reading frame (uORF) on its expression, thereby significantly increasing the expression level of the TaMIPS gene-encoded protein and effectively enhancing wheat disease resistance.

The invention discloses a stripe rust resistance gene YrRC1 derived from roegneria ciliata as well as an encoding protein and application thereof. The stripe rust resistance gene YrRc1 provided by the invention is derived from a roegneria ciliata 2Sc chromosome, the nucleotide sequence of the gene is SEQ ID NO.1, and the amino acid sequence of the gene is SEQ ID NO.2. The invention further discloses a preparation method of the stripe rust resistance gene YrRc1. The invention also protects the application of the YrRC1 in improving the disease resistance of plants. The YrRc1 gene is induced by puccinia striiformis to realize rapid up-regulation expression; a VIGS system is utilized to silence YrRc1 in a disease-resistant material DA2Sc to enhance the plant susceptibility; the YrRc1 gene is introduced into a stripe rust susceptible variety Fielder through a transgenic technology, so that the stripe rust resistance can be remarkably improved. Therefore, the YrRc1 can positively regulate and control the stripe rust resistance and has important significance on wheat disease-resistant breeding.

The application discloses a wheat scab-resistant gene linked molecular marker, a primer combination and application. The molecular marker W17 is linked to the scab-resistant gene, can be directly applied to wheat scab-resistant production practice, provides technical support for breeding of a wheat improved line carrying the scab-resistant gene, can improve the efficiency of molecular marker assisted selection, thereby speeds up research and utilization of a wheat scab-resistant excellent gene, and provides a new material for wheat disease-resistant genetic improvement. In addition, the application has the advantages of not being affected by external environmental factors, simple and convenient operation and the like.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of wheat VOZ transcription factors or their encoding genes in regulating crop stripe rust resistance. This invention systematically reveals for the first time the positive regulatory role of wheat transcription factors TaVOZ1 and TaVOZ2 in the immune response of wheat to stripe rust, enriching the functional spectrum of the VOZ family in plant immune regulation; simultaneously, this invention significantly enhances the resistance of overexpressed materials to wheat stripe rust fungus by overexpressing the encoding gene of wheat VOZ transcription factors. Pst The broad-spectrum resistance of wheat VOZ transcription factor has practical breeding application potential, thus proving that the wheat VOZ transcription factor encoding gene provided by this invention can be an important candidate for disease-resistant molecular breeding, providing a new approach and strategy for green prevention and control of wheat diseases.

The invention discloses a strain of schizophyllum commune DKK and application thereof in prevention and treatment of rice and wheat diseases, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is May 19, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.42010. The DKK strain is separated from persimmon fruits on which sporocarp grows. Researches show that the strain DKK inhibits the growth of rhizoctonia solani through antagonism and hyperparasitism; and the compound also has relatively good antagonistic activity on other pathogenic bacteria such as cochliobotrys miyabeanus, fusarium graminearum, wheat stem rot pathogen, potato late blight pathogen, tomato fusarium wilt pathogen, sclerotium fistulosum, strawberry botrytis cinerea, colletotrichum gloeosporioides and ligustrum japonicum leaf spot pathogen.

The invention belongs to the technical field of gene engineering, and relates to application of wheat receptor-like kinase TaSPR12 in improvement of wheat disease resistance. The wheat receptor kinase gene TaSPR12 is analyzed and screened by using a reverse genetics method, and the gene is induced to express by stripe rust germs. According to the present invention, the CRISPR-Cas9 gene editing technology is adopted to knock out the wheat receptor kinase gene TaSPR12 to create the function deletion mutant, and the resistance of the wheat stripe rust main epidemic race is improved; the invention determines that the wheat receptor kinase gene TaSPR12 plays a negative regulation role in wheat stripe rust resistance, and can be used for genetic improvement of wheat rust resistance.

The present application belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease resistance factor TaPKS26 in leaf rust prevention and treatment. The CDS sequence of the coding gene of the wheat disease resistance factor TaPKS26 is shown as SEQ ID NO:1, and the present application adopts an agrobacterium-mediated method to obtain a transgenic wheat strain overexpressing the gene TaPKS26. After being infected with the physiological race THT and PHT of leaf rust, the transgenic wheat strain overexpressing the gene TaPKS26 is compared with Fielder wheat, and the results show that the resistance of the transgenic wheat strain to wheat leaf rust is enhanced, and the expression amount of a disease resistance related marker gene is up-regulated, indicating that the wheat disease resistance factor TaPKS26 plays a positive regulation role in the defense response of wheat against leaf rust. The present application provides a new gene resource and technical idea for breeding of a leaf rust disease resistance variety.

This invention provides a method, device, and storage medium for identifying wheat diseases and pests in the field, belonging to the field of image processing. The method uses a neural network (DenseNet) to extract richer wheat disease and pest features; it optimizes the disease feature information output by each group of convolutions in DenseNet through feature pyramid fusion; it incorporates a convolutional attention module to optimize the output features of multiple convolutional layers to suppress the influence of complex background features on the performance of wheat disease and pest identification in the field; and it uses the Sobel filtering algorithm edge protocol header to detect the edge features of lesion areas, using these edge features to guide the model to predict the mask generation direction, thereby improving the identification speed of this invention. This method performs multiple feature optimizations on wheat disease and pest features through feature fusion and feature suppression during the feature extraction process, suppressing useless features introduced by complex backgrounds and highlighting the effective features of wheat disease and pest targets, thereby improving the accuracy of disease and pest identification.

This invention belongs to the field of genetic engineering technology and discloses the application of the transcription factor TaNAC67 in regulating wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown in SEQ ID NO: 1. This transcription factor can induce cell necrosis and plays a positive regulatory role in the interaction between wheat and stripe rust fungus. The gene encoding this transcription factor... TaNAC67 This is a disease resistance-related gene that is strongly induced by stripe rust infection in the incompatible interaction between plants and stripe rust fungi. Its nucleotide sequence is shown in SEQ ID NO: 2. Based on these characteristics, this invention further obtains an overexpressing gene. TaNAC67 The transgenic plants were tested and found to exhibit resistance to stripe rust. This invention provides genetic resources and technical support for the subsequent genetic improvement of wheat materials resistant to stripe rust.

This invention discloses a method for synergistic improvement of wheat quality and disease resistance based on the creation and utilization of the T1DL.1VS translocation line. Addressing the problem of low breeding efficiency caused by the dispersion of disease resistance and quality genes, this invention creates a novel T1DL.1VS translocation line that aggregates Pm67 / Glu-V1 by crossing the T1DL.1V#4S translocation line NAU195 carrying Glu-V1 with the T1DL.1V#5S translocation line NAU196 carrying Pm67. Recombinant single plants are screened in the F2 generation. Furthermore, utilizing the co-segregation characteristic of this translocation line with the wax repressor gene IW-V1, a dual-marker selection system combining morphological markers and the NAU1VS-1 molecular marker is established and introduced into a high-yielding variety background. This translocation line has no significant negative effect on yield traits, and the newly bred lines exhibit high resistance to powdery mildew and significantly improved bread processing quality, achieving synergistic and efficient improvement of wheat disease resistance and processing quality.

This invention relates to the field of genetic engineering technology, and more particularly to a molecular marker for detecting wheat leaf rust resistance genes LrN4B or LrN3B and its application. The molecular marker LrN4B-1 is located in the 578-585Mb region of chromosome 4BL, with a polymorphism of C / G. The molecular marker LrN3B-1 is located in the 4-8Mb region of chromosome 3BS, with a polymorphism of T / C. This invention obtains molecular markers linked to wheat leaf rust resistance genes LrN4B and LrN3B through the construction of genetic linkage maps and the screening and localization of molecular markers. These two molecular markers can be used to distinguish the LrN4B+LrN3B disease-resistant genotype, screen for wheat lines containing these two disease-resistant genes, accelerate the process of wheat disease-resistant breeding, and are of great significance in the field of breeding leaf rust-resistant wheat.

The invention discloses a wheat disease detection and identification method and system based on wavelet and posterior probability, and the method comprises the steps: S1, obtaining and preprocessing an original wheat disease image, inputting a reference SegFormer model, and extracting a multi-scale feature map through an encoder; s2, performing frequency domain attention processing based on two-dimensional discrete wavelet decomposition on the multi-scale feature map to generate a feature map after space enhancement; s3, obtaining an illumination intensity estimation value by using an illumination perception network, and calculating the Bayesian posterior probability of each category based on statistical association; s4, fusing the spatially enhanced feature map through a decoder to obtain fused features, predicting uncertainty through Dropout operation and convolution processing in combination with Bayesian posterior probability and by utilizing Monte Carlo sampling quantization, and outputting a segmentation result; wheat disease area segmentation is realized by combining the Bayesian posterior probability of frequency domain attention and illumination perception based on two-dimensional discrete wavelet decomposition and utilizing Monte Carlo sampling quantification to predict uncertainty.

This invention provides a method for breeding disease-resistant wheat varieties. [Solution] The wheat receptor-like protein kinase TaMLR1 provided by this application has a specific amino acid sequence, and the open reading frame (ORF) sequence encoding the wheat receptor-like protein kinase TaMLR1 is described in a specific nucleic acid sequence. In this disclosure, gene editing is performed on wild-type material Fielder to obtain TaMLR1 gene-edited mutant plants exhibiting resistance to wheat yellow rust, powdery mildew, and Fusarium head blight. This disclosure provides a novel technical approach for breeding disease-resistant varieties, confirms the negative regulatory role of this gene in wheat disease resistance, and provides a novel genetic resource for broad-spectrum disease-resistant breeding of wheat.

The invention discloses an omnibearing measuring device based on wheat agronomic traits, which comprises a box body and a shell located at the top end of the box body, a connecting column extending to the outside is arranged on the shell, and a detection piece for detecting the wheat agronomic traits is arranged at the top end of the connecting column. An adjusting assembly connected with the connecting column is arranged in the shell, a first lifting plate and a second lifting plate which are connected in a sliding mode are arranged in the shell, and limiting assemblies are arranged at the top end of the first lifting plate and the top end of the second lifting plate. The adjusting assembly can drive the detecting part to move through the connecting column, then the angle of the detecting part is adjusted according to the real-time condition, detected data are more accurate, meanwhile, the detecting part is matched for detecting pests on wheat plants, the proportion of wheat diseases and insect pests can be ingeniously recognized, and subsequent operation on the wheat plants is facilitated.

The invention relates to the related technical field of wheat disease prevention and control, in particular to a preparation method and application of carbon nanodots for wheat disease prevention and control, semen cassiae is used as a precursor, ethyl alcohol is used as a reaction solvent, the semen cassiae and the ethyl alcohol are mixed according to the mass ratio of 1: 2-1: 10 to obtain a mixed precursor, then the mixed precursor is placed in a reaction kettle to be sealed, and the carbon nanodots are prepared at the heating temperature of 140-180 DEG C for 1-2 h. The preparation method comprises the following steps: adding carbon nanodots into a reaction kettle, reacting for 2-8 hours, filtering the reacted solution to remove reaction residues, reserving the solution, putting the solution into a drying box, drying at 60 DEG C to obtain powder, re-dispersing the dried powder by using deionized water, dialyzing by using a dialysis bag with the molecular weight of 500 for 12 hours, and freeze-drying a sample by using a freeze-drying machine to obtain the carbon nanodot powder capable of being used for preventing and treating wheat diseases. The carbon nanodots can effectively generate active oxygen under excitation of visible light, disease fungi can be killed through a photophysical process, generation of drug resistance can be reduced to the maximum extent, and the disease incidence rate of wheat in the field can be effectively reduced by spraying the carbon nanodot solution to wheat.

This invention discloses the cloning and application of the wheat broad-spectrum powdery mildew resistance gene Pm26. The invention provides the Pm26 protein, a protein composed of the amino acid sequence shown in SEQ ID No. 3; it also provides the gene Pm26, a DNA molecule with the coding region shown in positions 3001-9489 of SEQ ID No. 1, or a DNA molecule with the coding region shown in SEQ ID No. 2; this invention provides gene localization, map-based cloning, and methods for identifying the biological function of the wheat broad-spectrum powdery mildew resistance gene Pm26. The wheat broad-spectrum powdery mildew resistance gene Pm26 can be widely used in plant fields such as wheat disease resistance genetic breeding, germplasm resource improvement, transgenic and genome editing breeding, and plays an important role in improving and enhancing the germplasm resources of crops such as wheat.