181 results about "Wheat diseases" patented technology

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.

The invention discloses a dCAPS molecular marker for identifying a wheat leaf rust resistance associated SNP site and application of the dCAPS molecular marker, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The invention develops a dCAPS molecular marker of SNP (Single Nucleotide Polymorphism) linked with a leaf rust resistance genetic locus QLr.hebau-1BS in a wheat adult-plant stage by virtue of 277 parts of wheat materials which have genotype data and are introduced from Turkey and in combination with a Chinese spring reference sequence. By utilizing the molecular marker, different wheat can be accurately and quickly detected, early-stage molecular-assisted selection of leaf rust resistant germplasm materials in the adult-plant stage is realized, and the wheat disease-resistant breeding efficiency is improved. The method solves the problem of high difficulty in detecting the leaf rust resistance gene in the wheat adult-plant stage in the prior art, and is suitable for the field of molecular genetic breeding.

The invention discloses a YOLOv8 improvement-based lightweight detection method for wheat diseases and insect pests in northwest weather, and relates to the technical field of artificial intelligence and machine vision monitoring. The method comprises the following steps: acquiring an original RGB image in a wheat filling period in a northwest dry land area environment; performing screening and Mosaic-8 data enhancement on the collected original image data set, and establishing a wheat disease and insect pest image data set; constructing an improved wheat lesion detection lightweight model based on YOLOv8, and optimizing a network structure; inputting the established wheat disease and insect pest image data set into the improved network model for training; testing the application of the improved network model in a wheat disease and insect pest image test data set, and continuously updating learning parameters of the improved YOLOv8 network model; the updated and trained YOLOv8 network model is used for monitoring a dry land area where wheat is planted in the Gansu province in real time, and the complexity and accuracy of the model are evaluated; and finally, real-time feedback of a model detection result is realized, and wheat plant diseases are dynamically updated to improve model precision.

The invention belongs to the technical field of gene engineering, and discloses a wheat susceptibility related gene, a protein and application. The wheat susceptibility related gene is a TaBRG10 gene and is derived from a wheat variety water source 11, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the TaBRG10 gene is as shown in SEQ ID NO: 2. The TaBRG10 gene is induced to express by stripe rust, and under the infection of stripe rust, the infection surface of a TaBRG10 gene silent plant infected by stripe rust is positively and obviously lower than that of a control plant, which indicates that the silent TaBRG10 gene improves the resistance of the plant to wheat stripe rust. The invention provides a novel germplasm material for the cultivation of the stripe rust resistant wheat variety.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention relates to a wheat leaf disease monitoring method based on the Internet of Things. The method comprises the following steps: acquiring a wheat leaf image by using an Internet of Things camera; transmitting the wheat leaf images to an upper computer, and setting a corresponding disease type label for each wheat leaf image in the upper computer; performing illumination balance compensation on each wheat leaf image to generate a compensated wheat leaf image; inputting the compensated wheat leaf image as a sample into a neural network for training to obtain a wheat disease monitoring model; and inputting the target wheat leaf image into the wheat disease monitoring model to obtain the disease type of the corresponding wheat. According to the invention, the wheat leaf image is automatically collected by using the Internet of Things camera, and the wheat leaf image is input into the wheat disease monitoring model to complete disease identification, so that the dependence on manual inspection is reduced, the labor cost is reduced, and the wheat disease monitoring precision is improved.

The invention relates to the technical field of genetic engineering, and discloses a fusarium pseudograminearum effect protein FpE01 and application thereof in wheat disease resistance. The amino acid sequence of the Fusarium pseudograminearum effect protein FpE01 is as shown in SEQ ID NO. 1. The nucleotide sequence of the coding gene of the Fusarium pseudograminearum effect protein FpE01 is as shown in SEQ ID NO. 2. The fusarium pseudograminearum effect protein FpE01 and the application thereof in wheat disease resistance are adopted, a knockout mutant of the coding gene FpE01 is constructed, the toxic function of the coding gene FpE01 in the process that wheat is infected by fusarium pseudograminearum is determined, then the effect protein is utilized to construct a wheat strain resistant to stem rot, and the wheat strain resistant to stem rot is obtained. And an excellent wheat material is provided for cultivation of basal stem rot resistant varieties.

The invention relates to the field of wheat disease detection, in particular to a wheat disease detection method based on deep learning. According to the method, foreground target extraction and multi-level feature extraction are performed on a to-be-detected wheat image by adopting an image processing technology based on deep learning so as to capture wheat foreground shallow layer features and deep layer features, and global interactive modeling is performed on the wheat foreground shallow layer features and the deep layer features by using a Transform architecture; the contextual information expression ability of the features is improved, and whether the wheat has diseases or not is intelligently identified through bidirectional attention joint perception of the features and the features. Therefore, the accuracy of wheat disease detection can be effectively improved, and the robustness of the model in a complex scene is enhanced.

The invention discloses a wheat stripe rust resistance regulation gene TaWAK2 as well as an encoding protein and application thereof, and belongs to the technical field of molecular biology. The wheat stripe rust resistance regulation gene TaWAK2 disclosed by the invention is as shown in a sequence table SEQ ID NO: 1, and the length of the wheat stripe rust resistance regulation gene TaWAK2 is 7042bp; the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 4 in a sequence table, and the length of the amino acid sequence is 693. Through a VIGS silencing technology and gene sequencing analysis, it is proved that the gene TaWAK2 plays an important role in wheat stripe rust resistance regulation and control, and the wheat stripe rust resistance is negatively regulated and controlled. The gene TaWAK2 disclosed by the invention can be applied to wheat stripe rust resistance breeding; research on the gene is beneficial to revealing a molecular genetic basis of wheat stripe rust resistance, and an important gene target is provided for wheat breeding for disease resistance.

The invention belongs to the technical field of wheat disease resistance breeding, and discloses a molecular marker for detecting wheat powdery mildew resistance QTL and application of the molecular marker. On the basis of a three-year group powdery mildew adult-plant stage resistance identification result, the powdery mildew resistance QTL is detected through a complete interval mapping method (ICIM) of IciMapping v4.2, two major QTLs, namely Qpm.histwrc-1AS and Qpm.histwrc-2BS-1, are detected in total, and the resistance contribution rates are 19.4%-34.5% and 19.7%-21.3%. Related molecular markers are developed through Bainong dwarf 58 sequencing data and can be applied to wheat disease-resistant molecular breeding, and a foundation is laid for exploring an Edinburgh-B disease-resistant gene and a resistance genetic mechanism.

The invention discloses application of an Fpg13 gene as a target in prevention and treatment of wheat diseases caused by fusarium pseudograminearum, and belongs to the technical field of biology. The invention researches and verifies that the Fpg13 protein is a fusarium pseudograminearum key toxic effect protein for the first time, and plays an important role in the process of infecting wheat by the fusarium pseudograminearum. By means of a PEG-mediated homologous recombination knockout technology, the effect protein Fpg13 gene of the fusarium pseudograminearum is knocked out, and the pathogenic ability of the fusarium pseudograminearum to wheat is weakened by the knockout mutant after knockout. The invention lays a theoretical foundation for developing the fusarium pseudograminearum small-molecule inhibitor by utilizing pathogenic factors of pathogenic bacteria, and has important significance for developing novel drugs of the pathogenic bacteria and inhibiting the generation of drug resistance.

The invention discloses an intelligent wheat disease diagnosis method and system based on a multi-modal large model. The method comprises the following steps: constructing a knowledge graph; obtaining a geographic position and current time of a user, positioning a wheat planting area based on the geographic position, calculating a wheat growth period in combination with the current time and the wheat planting area, and selecting a corresponding knowledge graph sub-graph according to the wheat growth period and the planting area; collecting texts and pictures input by a user through guiding type interaction; extracting text keywords, and identifying disease sites, disease types and disease symptoms in the pictures by using a deep learning model; fusing the text analysis result and the image analysis result to form a multi-modal feature; the optimized features are screened from the multi-modal features; a keyword set is decoded from the optimized features; generating a query statement based on the keyword set; and retrieving answers from the knowledge graph sub-graphs based on the query statements, and generating response contents based on the answers and feeding back the response contents to the user. The accuracy and efficiency of wheat disease diagnosis are improved.

The invention relates to the field of biomolecular detection, in particular to a KASP molecular marker related to wheat stripe rust resistance characters, a primer pair and application of the KASP molecular marker and the primer pair. According to the invention, 559 parts of common wheat germplasm resources are subjected to whole genome association analysis, a stable stripe rust resistance site QYr.hzau-3B. 1 is identified on a wheat 3B chromosome long arm, and a KASP molecular marker (KASP molecular marker KASP 3B2800) is developed aiming at the site and is used for screening large-scale materials. The KASP molecular marker provided by the invention provides a new site and a new marker for wheat stripe rust resistance breeding; meanwhile, the KASP molecular marker can be used for rapidly screening wheat germplasm containing the site, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The invention provides a wheat infected wheat ear segmentation method based on a perception enhanced gating network, and the method comprises the steps: obtaining a to-be-detected wheat image which comprises a plurality of wheat ears; inputting a to-be-detected wheat image into the trained perception enhanced gating network model to obtain a segmented image; the colors of healthy wheat ears, the colors of diseased wheat ears and the colors of background areas in the segmented images are different; the perception enhanced gating network model integrates a double-gating mechanism and a multi-scale expansion convolution block so as to realize accurate segmentation of wheat ears. According to the method, shallow local features are reserved in a semantic feature extraction branch through two different gating units, and deeper context features are extracted at the same time, so that various feature details are obtained, and a semantic segmentation model can still have excellent performance in a complex scene; and thus, a very accurate segmented image is obtained.

Cloning of a wheat broad-spectrum powdery mildew-resistant gene Pm26 and a use thereof. A Pm26 protein is a protein composed of the amino acid sequence represented by SEQ ID No. 3. Also provided is a gene Pm26, wherein a coding region is DNA molecules represented by positions 3001-9489 in SEQ ID No. 1, or the coding region is DNA molecules represented by SEQ ID No. 2. Further provided are gene mapping, map-based cloning and powdery mildew-resistant biological function identification methods for the wheat broad-spectrum powdery mildew-resistant gene PM26. The wheat broad-spectrum powdery mildew-resistant gene Pm26 can be widely used in plant fields of wheat disease-resistant genetic breeding, germplasm resource improvement, transgenic and genome editing breeding and the like, and plays an important role in improving and enhancing the germplasm resources of crops such as wheat.

The invention provides a molecular marker for identifying a wheat stalk rot resistant genetic locus and application thereof, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The molecular marker 4AS-AX-111108057 which is closely linked with the wheat stalk rot resistance genetic locus Qfcr. Hebau-4AS is developed by virtue of 360 parts of wheat materials which have 15K SNP chip data at home and abroad and in combination with a Chinese spring reference sequence. By utilizing the molecular marker, different wheat can be accurately and rapidly detected, early molecular assisted selection of stem rot resistant germplasm materials is realized, and the wheat disease-resistant breeding efficiency is improved. The method solves the problem that the detection difficulty of the wheat stem rot resistance gene in the prior art is large, and is suitable for the field of molecular genetic breeding.

The invention relates to the technical field of molecular breeding and phytopathology, in particular to a KASP molecular marker of a wheat stripe rust resistant gene and application of the KASP molecular marker. The invention particularly relates to a functional SNP (Single Nucleotide Polymorphism) marker of a wheat stripe rust resistance major gene Chr6B excavated on the basis of Meta-GWAS (Complete Genome Association Analysis), and application of a KASP (Kinase Amplified Sequence Protein) molecular marker system developed on the basis of an SNP locus in wheat disease-resistant germplasm resource screening, molecular marker-assisted breeding and gene pyramiding breeding. The major effect of the MQTL6B.2 site on the Chr6B chromosome in resistance phenotypic variation is disclosed for the first time, and the contribution rate is greater than or equal to 28.54%. The primer can distinguish a disease-resistant material from a susceptible material, and provides an effective molecular marking tool for subsequent disease-resistant breeding. A molecular marker detection tool can be provided for identification of stripe rust-resistant germplasm resources and breeding of disease-resistant varieties, the selection efficiency is improved, and the breeding process is accelerated.

The invention discloses a molecular marker and a primer for detecting a leaf rust resistant gene of wheat and application of the molecular marker and the primer. The method is not influenced by external environmental factors and is simple and convenient to operate. Primers of molecular markers Lsdau-33 and Xsdau3829 in close linkage with the wheat leaf rust resistance gene are utilized, PCR amplification electrophoresis detection is carried out, germplasm materials carrying the wheat leaf rust resistance gene can be screened out according to the size of a target band, the molecular marker can accelerate research and utilization of the excellent wheat leaf rust resistance gene, and the application prospect is wide. A new material is provided for wheat disease-resistant genetic improvement.

The invention belongs to the technical field of gene engineering, and relates to application of wheat receptor kinase TaSRK1 in improvement of wheat disease resistance. The wheat receptor kinase gene TaSRK1 is analyzed and screened by using a reverse genetics method, it is proved that TaSRK1 is induced to express by stripe rust germs, a CRISPR-Cas9 gene editing technology is adopted to knock out TaSRK1 to create a function deletion mutant, the resistance to main epidemic microspecies of wheat stripe rust is improved, and the resistance to the main epidemic microspecies of wheat stripe rust is improved. It is determined that the wheat receptor kinase gene TaSRK1 plays a negative regulation role in wheat stripe rust resistance, and the wheat stripe rust resistance gene TaSRK1 can be used for wheat stripe rust resistance genetic improvement.

The invention belongs to the technical field of genetic engineering, and relates to application of a wheat disease-resistant factor TaCBL4 in prevention and treatment of basal stem rot. A wheat strain capable of overexpressing the wheat disease-resistant factor gene TaCBL4 is obtained by adopting an agrobacterium-mediated method, and the condition that the wheat disease-resistant factor TaCBL4 plays a positive regulation role in the immune response of wheat stem rot resistance is known by comparing the morbidity condition and the fungal biomass condition of a receptor wheat Fielder infected with stem rot pathogens. On the basis, the wheat disease-resistant factor TaCBL4 provided by the invention can be used for creating a stem rot resistant wheat germplasm material, and plays an important role in prevention and treatment of wheat stem rot.

The invention provides a wheat disease identification method and device, and is applied to the technical field of crop disease identification. The method comprises the steps of obtaining a training sample set; determining mass-to-charge ratio signal intensity data of volatile organic compounds of the wheat leaf samples in the training sample set, determining a maximum characteristic value of each mass-to-charge ratio channel in the mass-to-charge ratio signal intensity data, mapping the maximum characteristic values of all the mass-to-charge ratio channels into pixel points, and obtaining a two-dimensional pixel matrix of the wheat leaf samples; extracting parent ions and pixel points where fragment ions corresponding to the parent ions are located from the two-dimensional pixel matrix, and rearranging the positions of the extracted pixel points in the two-dimensional pixel matrix to obtain a recombined feature map; and inputting the recombinant feature map into a wheat disease recognition model for network training, wherein the wheat disease recognition model is used for recognizing the disease type and infection degree of wheat according to the mass-to-charge ratio signal features in the recombinant feature map.

The invention belongs to the technical field of gene engineering, and discloses application of a transcription factor TaNAC67 in regulation and control of wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown as SEQ ID NO: 1, and the transcription factor can induce cell necrosis and exert a positive regulation function in the interaction process of wheat and puccinia striiformis. The coding gene TaNAC67 of the transcription factor is a disease-resistant related gene, is infected by stripe rust in non-affinity interaction of plants and stripe rust and is strongly induced to express, and the nucleotide sequence is shown as SEQ ID NO: 2. On the basis of the characteristics, a transgenic plant over-expressing the TaNAC67 gene is further obtained, and it is verified that the transgenic plant shows resistance to stripe rust. The invention provides gene resources and technical support for subsequent genetic improvement of stripe rust resistant wheat materials.

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.

The invention discloses a wheat stripe rust adult plant resistance gene Yr54, a specific functional molecular marker and application of the wheat stripe rust adult plant resistance gene Yr54 in wheat stripe rust resistance breeding, and belongs to the technical field of biology. A wheat stripe rust adult-plant resistance gene TaNLR2DL transgenic line is subjected to adult-plant-stage stripe rust resistance identification, and a result shows that compared with a non-TaNLR2DL gene line, the stripe rust resistance of the TaNLR2DL transgenic line is obviously improved, while a TaNLR2DL gene knockout line is contrary to the TaNLR2DL gene knockout line, so that the TaNLR2DL is a potential stripe rust resistance gene, and the TaNLR2DL gene knockout line can be used for identifying the stripe rust resistance in the adult-plant stage. The method can be used for genetic improvement of wheat stripe rust resistance. The invention further develops a functional specific molecular marker of TaNLR2DL, and the marker can quickly screen wheat germplasm containing TaNLR2DL, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The application discloses a wheat leaf rust resistance linkage SNP site, a dCAPS molecular marker, a primer, a kit and application, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The application provides a wheat leaf rust resistance linkage SNP site, wherein the SNP site is located at the 134261400th base on the 4D chromosome of a Chinese spring reference sequence with the version number of IWGSC_v1.1, and the polymorphism site is T / G. With the aid of 277 wheat materials introduced from Turkey with genotype data, and in combination with the Chinese spring reference sequence, a dCAPS molecular marker of the SNP site linked to the wheat adult stage leaf rust resistance genetic locus is developed, different wheat can be accurately and rapidly detected, early molecular assisted selection of the adult stage leaf rust resistance germplasm material is realized, and the efficiency of wheat disease resistance breeding is improved.

The invention discloses MlIW92 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to improve the resistance of wheat to powdery mildew. The MlIW92 protein disclosed by the invention is a protein of A1, A2 or A3 as follows: A1 is a protein of which the amino acid sequence is an amino acid sequence shown as SEQ ID No.2 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence as shown in SEQ ID No.2 in the sequence table, has 80% or more of identity with the protein as shown in A1, and is related to wheat powdery mildew resistance; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2. The protein disclosed by the invention is derived from wild two-grain wheat, and powdery mildew resistance gene resources of wheat disease resistance breeding work are enriched.

The invention belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease-resistant factor TaPKS26 in prevention and treatment of leaf rust. The CDS sequence of the encoding gene of the wheat disease-resistant factor TaPKS26 is shown as SEQ ID NO: 1, a transgenic wheat strain of the overexpressed gene TaPKS26 is obtained by adopting an agrobacterium-mediated method, and the transgenic wheat strain of the overexpressed gene TaPKS26 is proved to have enhanced resistance to wheat leaf rust compared with Fielder wheat after being infected with physiological races THT and PHT, and the wheat leaf rust resistance is improved. The expression quantity of the disease-resistant related marker gene is up-regulated, which indicates that the wheat disease-resistant factor TaPKS26 plays a positive regulation role in wheat leaf rust-resistant defensive reaction. The invention provides new gene resources and technical thoughts for the cultivation of leaf rust resistant varieties.

The invention discloses a molecular marker for detecting stripe rust and brown leaf rust resistant one-cause multiple-effect sites of wheat and a use method, and belongs to the technical field of nucleotide polymorphism and molecular markers. The technical problem to be solved by the invention is how to quickly and accurately screen or assist in screening wheat with stripe rust or / and leaf rust resistance or / and breed or assist in breeding wheat single plants with stripe rust or / and leaf rust resistance. The invention provides a composition for detecting single nucleotide polymorphism or genotype of a specific SNP site in a wheat genome, the SNP site can be the 47th nucleotide of a DNA fragment as shown in SEQ ID NO: 1 in the wheat genome, and the type of the nucleotide can be A or T. The primer composition and the molecular marker provided by the invention assist in wheat breeding for resisting stripe rust and leaf rust in wheat disease resistance breeding, greatly shorten the breeding period and play an important role in wheat breeding.