130 results about "Wheat diseases" patented technology

A cultivation method for high yield of selenium enrichment wheat is provided. Selenium fertilizer is mixed with seeds and applied during periods of straw pulling and flowering to enable wheat to produce physiological and biochemical reactions, so inorganic selenium can be absorbed and converted into organic selenium capable of being absorbed by human bodies; and enriched wheat grain become selenium enriched wheat. Through lab tests, content of selenium content of selenium of wheat grains can reach as high as 0.2mg/kg to 0.3mg/kg, a regulated value for human body health; wheat disease resistance property is enhanced according to scientific selenium fertilizer; 12 to 18% of wheat is increased compared with common wheat according to experts' test; and great benefit is produced to national grain production; and the method is worthy of promotion.

The invention belongs to the technical field of monitoring of crop diseases and in particular relates to a method for determining wheat head scab levels based on a cloud model. The method comprises the following steps: extracting disease areas, constructing color characteristics, generating a gibberella saubinetii color characteristic cloud model, establishing a comprehensive evaluation model andanalyzing the evaluation model. By utilizing the relationship between color changes in the disease areas and wheat diseased panicle rate levels in the gradual pathologic process of wheat head scab, the color characteristic cloud of the disease areas is obtained by combining with three numerical characteristics of the cloud model, the wheat head scab levels are subjected to non-rigid diversion by utilizing the cloud model, and comprehensive evaluation is realized.

The invention discloses a long-term preservation method of blumeria graminis strains, which comprises the steps of (1) the expanding propagation of monadic strains; (2) the collection of conidium; and (3) after silica gel is slowly dried for 5h, preservation in a refrigerator at a temperature of -70 DEG C. According to the method, the preservation time of blumeria graminis strains can be longer, is increased to 1 year from 10-12d by using conventional methods, and the method is simple, convenient and practical. The defects of the conventional preservation methods that operation is complicated, manpower and material resources consumption are seirous are overcome, so that the normal implementation of related researches on blumeria graminis strains is ensured. The method disclosed by the invention can be popularized and applied to wheat disease research and breeding mechanisms.

The invention relates to a wheat powdery mildew remote sensing monitoring method with a disease feature preprocessing function. Compared with the prior art, the method overcomes the defects of high feature redundancy and poor monitoring precision of wheat diseases. The method comprises the following steps of obtaining and processing remote sensing data; extracting feature variables; processing thefeature variables; building and optimizing a powdery mildew monitoring model; and obtaining a wheat powdery mildew remote sensing monitoring result. By combining relief and mRMR feature selection technologies with a support vector machine optimized through a genetic method, powdery mildew of regional scale is subjected to effective remote sensing monitoring.

The invention discloses a wheat disease resistance protein, an encoding gene and an application of the wheat disease resistance protein and the encoding gene in regulation of plant disease resistance. The wheat disease resistance protein TaZnF2 is a protein in A1), A2) or A3) as follows: A1) a protein with the amino acid sequence shown as sequence 2, A2) a protein related with the plant disease resistance, wherein the amino acid sequence shown in the sequence 2 in a sequence table is subjected to substitution and/or deletion and/or addition of one or more amino acid residues for acquisition of the protein, and A3) a fusion protein obtained by connecting labels to an N end or/and a C end of A1) or A2). Experiments prove that the resistance, to wheat sharp eyespot, of wheat genetically modified with TaZnF2 and having TaZnF2 gene overexpression is improved remarkably; while TaZnF2 gene expression in disease resistance wheat CI12633 is inhibited, resistance of plants to the wheat sharp eyespot is reduced, and the result that the TaZnF2 gene is a necessary disease resistance gene for the wheat sharp eyespot resistant reaction is proved.

The invention discloses a Bacillus sp. E1R-j, which is preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee on August 16th, 2007 with the preservation number of CGMCC No.2134; the invention further discloses a strain separating and identifying method, a thallus preparation method and a preparation method of an antimicrobial active substance, as well as applications of the E1R-j strain, the thallus and the antimicrobial active substance of the strain in controlling diseases including wheat take-all, Blumeria graminis and wheat yellow stripe rust as well as promoting seed germination and growth of wheat. The E1R-j strain is obtained from wheat root, has simple screening method, convenient operation, stable product quality, environmental protection and remarkable effects on controlling wheat take-all, Blumeria graminis and wheat yellow stripe rust, and does not have toxic side effect.

The invention discloses a wheat stripe rust resistance gene yrZ15-1370 as well as a molecular marker and application thereof. The wheat stripe rust resistance gene yrZ15-1370 is located on a long armof a 6A chromosome of wheat, and the physical position of the wheat stripe rust resistance gene yrZ15-1370 in a RefSeqv1.0 genome version is 601.47 Mb and 603.30 Mb. The SNP site of the molecular marker is located in the interval of yrZ15-1370, the polymorphism is A/G, and the molecular marker can accurately track the wheat stripe rust resistance gene yrZ15-1370. The invention further discloses aprimer group for identifying the wheat stripe rust resistance gene yrZ15-1370, the primer group provided by the invention can be used for rapidly screening out a wheat variety or strain with the wheatstripe rust resistance gene yrZ15-1370 for breeding, and the breeding process of a wheat disease-resistant variety can be greatly accelerated.

The invention relates to the fields of agricultural biotechnical engineering and wheat disease resistance genetic breeding, and in particular relates to a molecular marker interlocking with a wheat stripe rust resistant gene, an acquisition method and application thereof. The marker interlocking with the wheat stripe rust resistant gene is obtained through amplification by degenerate primers: an upstream primer 5-'AAGTGGAACAAGGTTACG-3', and a downstream primer 5'-GGIGGIGTIGGIAAIACIAC-3'. The marker can be used to assist selective breeding of wheat stripe rust resistance, and overcome the shortcomings in wheat stripe rust resistance identification in conventional breeding, such as vulnerability to environment impact, poor stability and repeatability, heavy workload, time and labor consumption and long breeding cycle, so as to speed up the breeding process of a wheat stripe rust resistance variety.

The invention discloses a wheat seed treating agent composition containing silthiopham, which solves the technical problems that a bactericidal spectrum is narrow, the usage amount per mu is high and the cost is high when silthiopham and fludioxonil are respectively used for preventing and treating wheat diseases. According to the technical scheme, the composition comprises the following components by weight percent: 10-80% of effective components, 5-15% of an auxiliary and 5-80% of fillers, wherein the effective components include silthiopham and fludioxonil by a weight ratio of (1:20)-(20:1). The wheat seed treating agent composition has the technical effects of being good in environment compatibility, low in toxicity and safe to humans and livestock; the composition is capable of preventing and treating the wheat diseases such as stinking smut, winter scald, snow mold, rhizoctonia solani kuhn, fusarium solani, septoria nodorum and flag smut which can be respectively prevented and treated by the silthiopham and the fludioxonil when the silthiopham and the fludioxonil are used respectively; besides, the composition has a remarkable synergism effect on gaeummanomyces; the usage amount per mu is lower than that when the silthiopham and the fludioxonil are used respectively, so that the usage amount is lowered and the cost is saved.

The invention discloses a special organic fertilizer for wheat plantation and a preparation method and application thereof. The organic fertilizer is prepared from, by mass, 50-70 parts of humic acid with the phosphorus content larger than or equal to 3% and the potassium content larger than or equal to 3%, 25-40 parts of bean pulp, 5-15 parts of rice chaff, 2-8 parts of radix sophorae tonkinensis residues, 5-15 parts of powdery urea, 10-20 parts of stems of cabbage, 20-30 parts of chicken manure, 25-35 parts of goose manure, 5-15 parts of patience dock root residues, 2-6 parts of fermentation microflora and 10-15 parts of microelement fertilizer. The organic fertilizer is quick and durable in fertilizer efficiency, and capable of restraining wheat diseases, promoting development of wheat root systems and improving yield and quality of wheat.

The invention belongs to the field of wheat disease resistance breeding, and in particular discloses a method for effectively breeding a durable resistance breeding material for stripe rust, wherein the material is obtained by combination of a seedling stage disease-resistant gene and adult-plant stage disease-resistant genes such as Yr18 by using high-pressure identification and multi-generation backcrossing techniques of hybrid microspecies. The method can provide an excellent intermediate material for rust-resistant breeding.

The invention discloses a wheat stripe rust resistance QTL molecular marker IWB12253 and an application thereof. The wheat stripe rust resistance QTL molecular marker IWB12253 is nucleotide shown at a51st site of a sequence 4 in a sequence table in a wheat genome, the wheat disease resistance molecular marker is T or C, experiments find that the stripe rust resistance of wheat with T at an SNP site IWB12253 is remarkably higher than that of wheat with C at the SNP site IWB12253, and the IWB12253 is related to the stripe rust of the wheat and can be used for detecting the stripe rust resistance of the wheat and breeding stripe rust resistant molecules.

The invention relates to a wheat seed coating agent which comprises the following active components: thiamethoxam and thifluzamide in the mass ratio of (2:1) to (20:1), preferably (6:1) to (9:1), wherein the weight percents of the active components, namely thiamethoxam and thifluzamide are 10%-90% of total weight, preferably 10%-70%. The invention further relates to application of the wheat seed coating agent for preventing and treating wheat pests and/or wheat diseases.

The invention relates to the technical field of plant gene engineering, in particular to the separation, cloning and applications of a TtLRR-STK gene the full length cDNA of which is 3081bp. The TtLRR-STK gene is separated from the emmer with high resistance to wheat diseases; the gene codes the receptor protein kinase of a leucine-rich area-serine/threonine; and the receptor protein kinase includes a plurality of parts such as an N-end conserved sequence, a leucine-rich structure domain (LRR), a middle transmembrane structure domain, a C-end protein kinase structure, and the like. The structure thereof is similar to a cloned rice resistance to bacterial blight protein Xa21 and is highly alike to one coding protein of the receptor kinase. The TtLRR-STK gene can be inserted into the downstream of promoters with different types for building a plant expression vector and carrying out plant disease resistance improvement through the gene engineering technology, and moreover, the cloning of the gene can also be used for manufacturing the gene chip of the wheat and be applied to the researches of wheat disease resistance, and the like.

The invention discloses a cultivating method for disease-resistant TaMYB-KW gene-transferred wheat, related biomaterials and application. TaMYB-KW protein is one of the proteins as follows: the first protein has an amino acid sequence shown in the sequence 2, the second protein is obtained in the mode that the amino acid sequence shown in the sequence 2 in a sequence table is subjected to substitution and/or deletion and/or addition of one or several amino acid residues and is related to the disease resistance of plants, and the third fusion protein is obtained by connecting labels to the N end or/and the C end of the first protein or the second protein. Experiments prove that the TaMYB-KW and a coding gene thereof can improve the disease resistance of the plants, and the TaMYB-KW gene is a wheat disease-resistant protein gene which is closely related to the sharp eyespot resistance and can be applied to genetic improvement of the plants.

The invention relates to the technical field of agricultural planting, in particular to compound fertilizer for preventing and treating wheat scab and an application method thereof. The compound fertilizer is prepared from the following raw materials in parts by weight: 5-15 parts of thiram wettable powder, 40-50 parts of straws, 28-35 parts of livestock manure, 14-29 parts of urea, 31-36 parts ofmonopotassium phosphate, 2-5 parts of borax, 2-4 parts of borax, 1-3 parts of magnesium sulfate, 2-6 parts of ferrous sulfate, 9-15 parts of sodium selenite, 40-60 parts of Chinese herbal medicine extract, 0.5-2.5 parts of a fermentation inoculant, 1-2 parts of cellulase and 1-4 parts of brown sugar. The compound fertilizer for preventing and treating wheat scab and the application method thereofare provided by the invention, and various medicaments are perfectly combined together by combining a traditional Chinese medicine medicament with sterilization and parasite expelling effects according to different action mechanisms of the medicaments, so that various wheat diseases such as the wheat scab can be prevented and treated, physical and chemical properties of soil are also improved, fertilizer utilization rate and soil fertility are improved, beneficial microflorae in the soil are enriched, and pollution of harmful substances on the soil is reduced.

The invention discloses a wheat disease resistance and heading regulation gene TaCOK, a related biological material and an application. The TaCOK is any one of the following proteins: A1) a protein with an amino acid sequence as shown in SEQ ID No.2; A2) a protein which is obtained by substituting and/or deleting and/or adding one or more amino acid residues to the amino acid sequence limited in A1), has 90% or more identity with the protein shown in A1) and is related to plant disease resistance; and A3) a fusion protein obtained by connecting a label to the N terminal or/and C terminal of A1) or A2). The TaCOK and a coding gene thereof provided by the invention can be used for improving disease resistance of plants and regulating and controlling the heading of the plants by regulating and controlling the expression effect of vernalization genes and vernalization related genes, and have important significance for cultivating new plant varieties which are resistant to diseases and havewide adaptability.

The invention provides a wheat stripe rust monitoring method based on the spectral information and meteorological data. The method comprises steps of a time phase remote sensing image of a to-be-monitored area being acquired; obtaining an image of the wheat planting area from the time-phase remote sensing image; obtaining a first vegetation spectral index of the wheat planting area in a first preset time and a second vegetation spectral index of the wheat planting area in a second preset time, and performing normalization calculation on the first vegetation spectral index and the second vegetation spectral index of the wheat planting area to obtain a normalized vegetation spectral index change value of the wheat planting area; obtaining meteorological information data of the wheat plantingarea in a preset time period; and inputting the normalized vegetation spectral index change value and the meteorological information data of the wheat planting area into a constructed wheat stripe rust remote sensing monitoring model to obtain a wheat disease index of the wheat planting area, thereby judging a wheat stripe rust monitoring result of the wheat planting area. According to the method, the infection condition of the wheat stripe rust in each wheat area can be judged in a large range.

Pm23 is a wheat powdery mildew resistant gene tested in an ordinary wheat strain in China. The gene represents high resistance or immune on the powdery mildew prevalent in a plurality of wheat areas in the world, located to 5A chromosome. In the research, the wheat strain Pm23 with the disease resistant gene Pm23 is used as a disease resistant parent and the wheat breed Chancellor without any disease resistant gene as a susceptible parent for confecting hybridized combination, and RAPD analysis is carried out on the resistance gene Pm23 by a bulked segregant analysis (BSA) method. The augmentation result of 350 ten-base random primers in an induction resistant pool shows that coupling phase marker OPE051100 may be tightly linked with Pm23. RAPD analysis on F2 segregation population shows that the linkage distance between the marker and the Pm23 gene is 10.65+3.25cM. The marker can be effectively used in the molecular marker-assisted selection of wheat disease resistant breeding.

The invention discloses a green-keeping anti-ageing agent in middle and later grouting period of wheat and an operating method of efficient production increasing technique, wherein when wheat enters into the later grouting period, namely, from wheat grain forming initial period to wheat milk ripeness initial period, a bactericide tebuconazole (or propiconazole or triazolone or diniconazole or difenoconazole or epoxiconazole), a plant growth regulator gibberellic acid and a leaf fertilizer: a mixture of urine and monopotassium phosphate are sparyed, so that the wheat is green-keeping, anti-aging and mature. The exogrnous substances are fresh golden yellow when wheat is mature. The control efficiency to wheat diseases reaches 90.17-100%, the thousand seed weight of wheat is increased by 4.12-6.62g, and the increase production per mu (a unit of area) is 42.90-87.96kg.

The present invention discloses a method for preventing fungal wheat diseases by using biological agent and chemical resistance inducer. The method is capable to overcome the defects of other methods. The method includes the following steps, step 1:during plowing before sowing, 300g soil activator Medina for each mu of soil is mixed with proper amount of water and sprayed into the plowed soil; proper amount of plant chemical resistance inducer Isolane regulator is used for seed dressing; step 2: during the tillering stage of the wheat, 20ml Isolane regulator is used for one mu of leaf sprinkling or root irrigating during the tillering stage of the wheat; step 3: during the greening stage, 20ml Isolane regulator is used to sprinkle one mu of foliages once; step 4: during the grouting stage, another 20ml Isolane regulator is used to sprinkle the foliages once; according to the practice, the diseases seldom take place during the fully growing stage, and the average yield increases by about 30 percent.

The present invention relates to plant gene engineering technology, and is especially one kind of wheat disease resisting gene Lr-L1 and its application. The wheat disease resisting gene Lr-L1 has cDNA of whole length 2326 bp, including 5'-noncoding area of 135 bp, 3'-noncoding area of 249 bp, coding area of 1911 bp, terminator, and polyadenyl acid tail of 28 bp. The 3'-noncoding area includes includes 3 typical tailing signal sequences and can reinforce the tailing process after gene transcription. The wheat disease resisting gene Lr-L1 may be inserted into the downstream of different types of promoter to constitute plant expression vector for plant disease resisting improvement in gene engineering technology, and may be cloned for making wheat gene chip for wheat disease resistance and other research.

The disease-preventing and yield-increasing disinfectant consists of diniconazole, thiram, assistants and humic acid in certain weight proportion. The assistants include lubricant, dispersant and filler in certain weight proportion. When it is applied, the disease-preventing and yield-increasing disinfectant is compounded into water solution for dressing seed or spraying to foliage. The disease-preventing and yield-increasing disinfectant can prevent and control many kinds of wheat diseases, decrease the harm of dry hot wind and raise the yield of wheat.

The invention relates to the field of wheat disease resistance, and specifically relates to a BFR protein associated with resistance of wheat to powdery mildew as well as a coding gene and applicationthereof. The BFR protein associated with resistance of wheat to powdery mildew has an amino acid sequence as shown in sequence 7, or a sequence constructed by replacing one or more amino acids in theamino acid sequence shown in sequence 7. Expression of the BFR protein in wheat leaves is reduced by adopting a virus-induced gene silencing technology and a CRISPR/Cas9 gene editing technology so asto have reaction type of infection of a powdery-mildew-resistant wheat variety, xiao-bai-dong (Triticum aestivum L.), on an avirulent strain E18 changed from grade 0-1 to grade 3-4. Over-expression of the BFR gene in wheat leaves is allowed by adopting biolistic transformation; and thus, pathogen spore germination of a virulent strain E18 on leaves of the wheat variety Kelong-199 is significantlyinhibited.