107 results about "Wheat diseases" patented technology

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention discloses application of an Fpg13 gene as a target in prevention and treatment of wheat diseases caused by fusarium pseudograminearum, and belongs to the technical field of biology. The invention researches and verifies that the Fpg13 protein is a fusarium pseudograminearum key toxic effect protein for the first time, and plays an important role in the process of infecting wheat by the fusarium pseudograminearum. By means of a PEG-mediated homologous recombination knockout technology, the effect protein Fpg13 gene of the fusarium pseudograminearum is knocked out, and the pathogenic ability of the fusarium pseudograminearum to wheat is weakened by the knockout mutant after knockout. The invention lays a theoretical foundation for developing the fusarium pseudograminearum small-molecule inhibitor by utilizing pathogenic factors of pathogenic bacteria, and has important significance for developing novel drugs of the pathogenic bacteria and inhibiting the generation of drug resistance.

The invention discloses an intelligent wheat disease diagnosis method and system based on a multi-modal large model. The method comprises the following steps: constructing a knowledge graph; obtaining a geographic position and current time of a user, positioning a wheat planting area based on the geographic position, calculating a wheat growth period in combination with the current time and the wheat planting area, and selecting a corresponding knowledge graph sub-graph according to the wheat growth period and the planting area; collecting texts and pictures input by a user through guiding type interaction; extracting text keywords, and identifying disease sites, disease types and disease symptoms in the pictures by using a deep learning model; fusing the text analysis result and the image analysis result to form a multi-modal feature; the optimized features are screened from the multi-modal features; a keyword set is decoded from the optimized features; generating a query statement based on the keyword set; and retrieving answers from the knowledge graph sub-graphs based on the query statements, and generating response contents based on the answers and feeding back the response contents to the user. The accuracy and efficiency of wheat disease diagnosis are improved.

The invention belongs to the technical field of genetic engineering, and relates to application of a wheat disease-resistant factor TaCBL4 in prevention and treatment of basal stem rot. A wheat strain capable of overexpressing the wheat disease-resistant factor gene TaCBL4 is obtained by adopting an agrobacterium-mediated method, and the condition that the wheat disease-resistant factor TaCBL4 plays a positive regulation role in the immune response of wheat stem rot resistance is known by comparing the morbidity condition and the fungal biomass condition of a receptor wheat Fielder infected with stem rot pathogens. On the basis, the wheat disease-resistant factor TaCBL4 provided by the invention can be used for creating a stem rot resistant wheat germplasm material, and plays an important role in prevention and treatment of wheat stem rot.

The invention belongs to the technical field of gene engineering, and discloses application of a transcription factor TaNAC67 in regulation and control of wheat disease resistance. The amino acid sequence of the TaNAC67 transcription factor is shown as SEQ ID NO: 1, and the transcription factor can induce cell necrosis and exert a positive regulation function in the interaction process of wheat and puccinia striiformis. The coding gene TaNAC67 of the transcription factor is a disease-resistant related gene, is infected by stripe rust in non-affinity interaction of plants and stripe rust and is strongly induced to express, and the nucleotide sequence is shown as SEQ ID NO: 2. On the basis of the characteristics, a transgenic plant over-expressing the TaNAC67 gene is further obtained, and it is verified that the transgenic plant shows resistance to stripe rust. The invention provides gene resources and technical support for subsequent genetic improvement of stripe rust resistant wheat materials.

This invention discloses the application of the wheat gene TaALD1 in improving wheat stripe rust resistance, belonging to the field of biotechnology. The key technical points are: the nucleotide sequence of TaALD1 is shown in SEQ ID No. 1. In this invention, TaALD1 is upregulated after infection with stripe rust fungus, and virus-induced gene silencing confirms that TaALD1 plays a positive regulatory role in wheat stripe rust resistance. Simultaneously, transgenic plants of the ALD1 gene significantly enhance wheat's resistance to stripe rust, showing promising application prospects in breeding disease-resistant wheat varieties and laying an important foundation for wheat disease-resistant breeding.

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.

The invention discloses a wheat stripe rust adult plant resistance gene Yr54, a specific functional molecular marker and application of the wheat stripe rust adult plant resistance gene Yr54 in wheat stripe rust resistance breeding, and belongs to the technical field of biology. A wheat stripe rust adult-plant resistance gene TaNLR2DL transgenic line is subjected to adult-plant-stage stripe rust resistance identification, and a result shows that compared with a non-TaNLR2DL gene line, the stripe rust resistance of the TaNLR2DL transgenic line is obviously improved, while a TaNLR2DL gene knockout line is contrary to the TaNLR2DL gene knockout line, so that the TaNLR2DL is a potential stripe rust resistance gene, and the TaNLR2DL gene knockout line can be used for identifying the stripe rust resistance in the adult-plant stage. The method can be used for genetic improvement of wheat stripe rust resistance. The invention further develops a functional specific molecular marker of TaNLR2DL, and the marker can quickly screen wheat germplasm containing TaNLR2DL, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The application discloses a wheat leaf rust resistance linkage SNP site, a dCAPS molecular marker, a primer, a kit and application, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The application provides a wheat leaf rust resistance linkage SNP site, wherein the SNP site is located at the 134261400th base on the 4D chromosome of a Chinese spring reference sequence with the version number of IWGSC_v1.1, and the polymorphism site is T / G. With the aid of 277 wheat materials introduced from Turkey with genotype data, and in combination with the Chinese spring reference sequence, a dCAPS molecular marker of the SNP site linked to the wheat adult stage leaf rust resistance genetic locus is developed, different wheat can be accurately and rapidly detected, early molecular assisted selection of the adult stage leaf rust resistance germplasm material is realized, and the efficiency of wheat disease resistance breeding is improved.

The invention discloses MlIW92 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to improve the resistance of wheat to powdery mildew. The MlIW92 protein disclosed by the invention is a protein of A1, A2 or A3 as follows: A1 is a protein of which the amino acid sequence is an amino acid sequence shown as SEQ ID No.2 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence as shown in SEQ ID No.2 in the sequence table, has 80% or more of identity with the protein as shown in A1, and is related to wheat powdery mildew resistance; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2. The protein disclosed by the invention is derived from wild two-grain wheat, and powdery mildew resistance gene resources of wheat disease resistance breeding work are enriched.

The invention belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease-resistant factor TaPKS26 in prevention and treatment of leaf rust. The CDS sequence of the encoding gene of the wheat disease-resistant factor TaPKS26 is shown as SEQ ID NO: 1, a transgenic wheat strain of the overexpressed gene TaPKS26 is obtained by adopting an agrobacterium-mediated method, and the transgenic wheat strain of the overexpressed gene TaPKS26 is proved to have enhanced resistance to wheat leaf rust compared with Fielder wheat after being infected with physiological races THT and PHT, and the wheat leaf rust resistance is improved. The expression quantity of the disease-resistant related marker gene is up-regulated, which indicates that the wheat disease-resistant factor TaPKS26 plays a positive regulation role in wheat leaf rust-resistant defensive reaction. The invention provides new gene resources and technical thoughts for the cultivation of leaf rust resistant varieties.

The invention discloses a molecular marker for detecting stripe rust and brown leaf rust resistant one-cause multiple-effect sites of wheat and a use method, and belongs to the technical field of nucleotide polymorphism and molecular markers. The technical problem to be solved by the invention is how to quickly and accurately screen or assist in screening wheat with stripe rust or / and leaf rust resistance or / and breed or assist in breeding wheat single plants with stripe rust or / and leaf rust resistance. The invention provides a composition for detecting single nucleotide polymorphism or genotype of a specific SNP site in a wheat genome, the SNP site can be the 47th nucleotide of a DNA fragment as shown in SEQ ID NO: 1 in the wheat genome, and the type of the nucleotide can be A or T. The primer composition and the molecular marker provided by the invention assist in wheat breeding for resisting stripe rust and leaf rust in wheat disease resistance breeding, greatly shorten the breeding period and play an important role in wheat breeding.

The invention belongs to the technical field of genetic engineering, and relates to application of a wheat disease-resistant factor TaCIPK10 in prevention and treatment of basal stem rot. A wheat strain capable of overexpressing the wheat disease-resistant factor gene TaCIPK10 is obtained by adopting an agrobacterium-mediated method, and the condition that the wheat disease-resistant factor gene TaCIPK10 plays a positive regulation role in the immune response of wheat stem rot resistance is known by comparing the morbidity condition and the fungal biomass condition of a receptor wheat Fielder infected with stem rot pathogens. On the basis, the wheat disease-resistant factor TaCIPK10 provided by the invention can be used for creating a stem rot resistant wheat germplasm material, and plays an important role in prevention and treatment of wheat stem rot.

The invention belongs to the technical field of molecular biology, and relates to a KASP molecular marker for wheat stripe rust resistance gene detection and application. The KASP molecular marker XK6B-5 disclosed by the invention is obtained by utilizing a specific primer group as shown in SEQ ID NO.2-4 for amplification; the KASP molecular marker XK6B-5 is closely linked with a wheat stripe rust resistance gene YrZT94.1, and has a co-separation characteristic, and the polymorphism of the XK6B-5 is G / T. The KASP molecular marker provided by the invention can accurately track the stripe rust resistance gene YrZT94.1 of wheat, performs molecular marker-assisted selection, and improves the breeding efficiency of wheat disease resistance. The method can be applied to screening of the stripe rust resistant gene YrZT94.1 in wheat germplasm resources in a simple, rapid and high-throughput manner, so that the method is applied to molecular design breeding, and the wheat breeding process is greatly accelerated.

The invention belongs to the technical field of molecular biology, and relates to a KASP molecular marker for detecting a wheat stripe rust resistance gene YrZT94.2 and application of the KASP molecular marker. The KASP molecular marker XK7B-32 disclosed by the invention is obtained by utilizing a specific primer group as shown in SEQ ID NO.2-4 for amplification; the KASP molecular marker XK7B-32 is closely linked with a wheat stripe rust resistance gene YrZT94.2, and has a co-separation characteristic, and the polymorphism of the XK7B-32 is C / G. The KASP molecular marker provided by the invention can accurately track the stripe rust resistance gene YrZT94.2 of wheat, performs molecular marker-assisted selection, and improves the breeding efficiency of wheat disease resistance. The method can be simply and quickly applied to screening of the stripe rust resistant gene YrZT94.2 in wheat germplasm resources with high throughput, so that the method is applied to molecular design breeding, and the wheat breeding process is greatly accelerated.

This invention belongs to the field of genetic engineering technology and relates to the application of wheat receptor kinase TaSPR12 in improving wheat disease resistance. This invention utilizes reverse genetics methods to analyze and screen wheat receptor kinase genes. TaSPR12 This gene is induced to express by the stripe rust pathogen. The wheat receptor kinase gene was knocked out using CRISPR-Cas9 gene editing technology. TaSPR12 The created loss-of-function mutants exhibited enhanced resistance to the main prevalent races of wheat stripe rust. This invention identified the wheat receptor kinase gene. TaSPR12 It plays a negative regulatory role in wheat resistance to stripe rust and can be used for genetic improvement of wheat resistance to rust.

The invention belongs to the field of genetic engineering, and particularly relates to a wheat stripe rust resistance germplasm resource identification and breeding method. The CDS nucleotide sequence of the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the wheat stripe rust resistance gene Yrym112 is as shown in SEQ ID NO. 2. The developed functional molecular marker Yrym112-InDel comprises an upstream primer sequence as shown in SEQ ID NO. 3 and a downstream primer sequence as shown in SEQ ID NO. 4. A material containing the Yrym112 gene can be divided into a disease-resistant genotype and a susceptible genotype according to the size of an amplified band. The molecular marker is used for identifying or breeding wheat stripe rust resistant germplasm resources, or wheat gene Yr5 typing and wheat molecular marker assisted breeding are used, and the breeding process of wheat disease resistant varieties is accelerated.

The application relates to the technical field of genetic engineering, and discloses application of a transcription factor TabHLH168 protein in wheat disease resistance. The amino acid sequence of the TabHLH168 protein is shown as SEQ ID NO. 1; the coding gene sequence of the TabHLH168 protein is shown as SEQ ID NO. 2. The TabHLH168 protein is a target protein of a false smut fungus effect protein FpE02. The application uses the above-mentioned application of the transcription factor TabHLH168 protein in wheat disease resistance, overexpresses the coding gene of the TabHLH168 protein, obtains transgenic wheat with stable heredity and wheat stem base rot resistance, provides a new solution for carrying out wheat disease resistance breeding work, and has important significance in the field of wheat breeding with disease resistance.

The invention relates to an image recognition and expert system fused wheat disease intelligent diagnosis system and method, and belongs to the technical field of wheat disease diagnosis. Different processing modes are adopted for different detection results; for the condition that the disease image features are detected and the corresponding disease is unique, the disease result is directly output; a second expert system module performs further identification on the condition that the detected disease image features are not unique corresponding to the disease; and for the condition that no disease image feature is detected, the first expert system module performs diagnosis, if the disease type is diagnosed, a disease result is output, and if the disease type is not diagnosed, the manual judgment module can perform further diagnosis. According to the system and the method, all types of scenes such as obvious image features, similar image features, non-image feature dominant and unknown diseases can be covered, and intelligent diagnosis of various types of wheat diseases is realized.

The invention belongs to the technical field of genetic engineering breeding, and discloses a wheat disease-resistant factor gene TaPsaF and application thereof. A TaPsaF gene sequence is analyzed, and a TaPsaF-GFP fusion protein is constructed, so that the localization of the TaPsaF-GFP fusion protein in cell chloroplast is determined. An overexpression and silence vector of the TaPsaF gene is successfully constructed by using a gene engineering technology, and wheat is transformed by using an agrobacterium-mediated method to obtain a transgenic wheat material. Functional verification shows that over-expression of the TaPsaF gene can significantly enhance the resistance of wheat to stripe rust, and silencing of the gene leads to reduction of the resistance. Gene resources and a technical path are provided for wheat stripe rust resistance molecular breeding, the blank of the TaPsaF protein in biological stress response mechanism research is filled up, and the TaPsaF protein has important significance for guaranteeing food safety.

The application discloses a wheat disease-resistant gene TaLOX and application thereof in regulating resistance to stripe rust. TaLOX The application overexpresses the wheat gene in wheat, inoculates the overexpressed wheat plants with stripe rust, and observes the resistance phenotype of the overexpressed wheat plants to the stripe rust (Puccinia striiformis f. Puccinia striiformis sp. tritici ). TaLOX The results show that, compared with a control group, the amount of spores of the stripe rust on the surface of the overexpressed wheat plants is significantly reduced, the disease index and the fungal biomass are significantly reduced, and the mycelium length is significantly shorter than that of the control group, indicating that the gene positively regulates the resistance of the wheat to the stripe rust. TaLOX The application has application prospects in breeding of wheat varieties resistant to stripe rust and improvement of the resistance level of the wheat to the stripe rust.

The application discloses a wheat salicylic acid receptor NPR3 gene and application thereof in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The application discloses application of a wheat salicylic acid receptor NPR3 gene or related biological materials thereof, and the application is as follows: A1, improving the resistance of wheat to stripe rust; A2, improving the resistance of wheat to powdery mildew; A3, improving the resistance of wheat to leaf rust; and the nucleotide sequence of the wheat salicylic acid receptor NPR3 gene is shown as SEQ ID NO. 3. The results of the application show that NPR3 gene knockout lines are resistant to different stripe rust physiological races CYR17, CYR32, CYR33 and CYR34, and further resistance identification shows that the NPR3 gene knockout lines are resistant to powdery mildew and leaf rust. The application provides theoretical guidance and genetic gene resources for wheat disease resistance breeding.

The invention discloses a candidate gene AetNLR2CIae8 of a powdery mildew resistant gene pmAeCIae82DS derived from aegilops tauschii as well as a protein coded by the candidate gene AetNLR2CIae8 and application of the candidate gene AetNLR2CIae8, and the nucleotide sequence of the candidate gene AetNLR2CIae8 of the powdery mildew resistant gene derived from the aegilops tauschii is as shown in SEQ ID NO. 1. 1, and amino acid sequences coded by the gene are respectively as shown in SEQ ID NO. 2. The pmAeCIae82DS gene sequence belongs to the protection range of the invention. The invention also protects the application of the candidate gene AetNLR2CIae8 of the pmAeCIae82DS in the improvement of the disease resistance of the plants. The AetNLR2CIae8 is transformed into the susceptible wheat variety Yangmai 158 by utilizing a transient overexpression system, so that the aspirator index of powdery mildew can be obviously reduced; when the VIGS system is used for respectively silencing AetNLR2CIae8 in the aegilops sphaeroides powdery mildew resistant material TX11-1 containing the pmAeCIae82DS, the susceptibility of plants can be improved. Therefore, the AetNLR2CIae8 can positively regulate and control the wheat powdery mildew resistance and is an important gene resource for wheat disease-resistant breeding.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat VOZ transcription factor or a coding gene thereof in regulation and control of stripe rust resistance of crops. The invention systematically reveals that wheat transcription factors TaVOZ1 and TaVOZ2 play a positive regulation role in immune response of wheat to stripe rust for the first time, and enriches a functional spectrum of a VOZ family in plant immune regulation; meanwhile, the broad-spectrum resistance of an overexpression material to wheat stripe rust (Pst) is remarkably enhanced by overexpressing the encoding gene of the wheat VOZ transcription factor in wheat, and the wheat VOZ transcription factor has practical breeding application potential, so that the wheat VOZ transcription factor encoding gene provided by the invention can be used as an important candidate for breeding disease-resistant molecules; a new way and a new strategy are provided for green prevention and control of wheat diseases.

The application discloses a candidate gene NLR-G4 derived from a Timopheevi wheat powdery mildew resistance gene Pm6 and application thereof.The nucleotide sequence of the candidate gene NLR-G4 derived from the Timopheevi wheat powdery mildew resistance gene is shown in SEQ ID NO.1.The application also protects the application of the candidate gene NLR-G4 of Pm6 in improving plant disease resistance.Using a transient overexpression system, the NLR-G4 can be transformed into a susceptible wheat variety Yangmai 158, so that the sinker index can be significantly reduced; using a VIGS system, the NLR-G4 can be silenced in a disease-resistant material IGV1-465, so that the plant disease susceptibility is enhanced.Therefore, the NLR-G4 positively regulates the powdery mildew resistance, and has important significance for wheat disease-resistant breeding.

The present invention discloses a wheat S-adenosylmethionine synthase, TaSAMS-6B2, and its use in improving wheat resistance to scab. The wheat S-adenosylmethionine synthase gene, TaSAMS-6B2, provided by the present invention, can significantly improve the disease resistance of susceptible wheat, providing an effective technical means for preventing and controlling scab in production. This, in turn, accelerates wheat disease-resistant breeding and improves disease prevention and control measures, thus having broad application prospects.

The invention belongs to the technical field of molecular genetic breeding, and particularly relates to an SNP site for identifying wheat sharp eyespot resistance, a CAPS molecular marker primer and application of the SNP site and the CAPS molecular marker primer. The SNP (Single Nucleotide Polymorphism) sites of the wheat sharp eyespot resistance disclosed by the invention comprise 6A-AX-111665286 and / or 6A-AX-110674425, and the SNP sites of the wheat sharp eyespot resistance disclosed by the invention comprise the The 6A-AX-111665286 is located at the 600397542bp position of a wheat 6AL chromosome, and T / G polymorphism exists in the 6A-AX-111665286. The 6A-AX-110674425 is located at the 600382189th bp position of a wheat 6AL chromosome, and the A / G polymorphism exists in the 6A-AX-110674425. The molecular marker developed by using the SNP site can accurately and rapidly detect the sheath blight resistance of wheat, can be used for early molecular assisted selection of disease-resistant wheat, and improves the breeding efficiency of wheat disease resistance.

This invention provides a method and apparatus for identifying wheat diseases, applied in the field of crop disease identification technology. The method includes: acquiring a training sample set; determining the mass-to-charge ratio (M / C ratio) signal intensity data of volatile organic compounds in wheat leaf samples from the training sample set, and determining the maximum eigenvalue of each M / C ratio channel in the M / C ratio signal intensity data; mapping the maximum eigenvalues ​​of all M / C ratio channels to pixels to obtain a two-dimensional pixel matrix of wheat leaf samples; extracting the parent ion and its corresponding fragment ions from the two-dimensional pixel matrix, and rearranging the positions of the extracted pixels in the two-dimensional pixel matrix to obtain a reconstructed feature map; inputting the reconstructed feature map into a wheat disease identification model for network training, wherein the wheat disease identification model is used to identify the disease type and severity of wheat based on the M / C ratio signal features in the reconstructed feature map.

The invention discloses a molecular marker related to wheat curly mite resistance and application of the molecular marker, and belongs to the technical field of wheat breeding for disease resistance. According to the invention, a nucleotide fragment related to wheat curly mite resistance is obtained through screening, and based on a nucleotide sequence of the fragment, the invention also designs a molecular marker for specific detection of the nucleotide fragment. The nucleotide fragment and the detection molecular marker thereof are not limited by environmental conditions, PCR amplification is carried out through the primer pair, plant germplasm resources with wheat curly mite resistance can be accurately screened according to the existence of target bands, the breeding efficiency is improved, and the breeding process is accelerated.