1735results about "Microorganism preservation" patented technology

The present disclosure provides water-soluble film packages composed of a water-soluble film, where the packages include: one or more compartment(s) including dehydrated microbes. The disclosure further provides methods of production of the water-soluble film packages, and methods of application thereof. The water-soluble packages of the disclosure enable convenient and accurate application of microbial dry powders to plants, promote uniform dispersion of powdered microbes, enhance microbial shelf life, and enhance the survival of microbes on seed, while being environmentally sustainable.

The invention relates to lactobacillus rhamnosus CBBM005 and application thereof in products for balancing intestinal flora, improving immunity and improving oral cavity, and belongs to the technical field of microorganisms. The lactobacillus rhamnosus CBBM005 is separated from human intestinal secretion, the lactobacillus rhamnosus CBBM005 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation time is March 20, 2025, and the preservation number is CGMCC NO.33904. The lactobacillus rhamnosus CBBM005 has the characteristics of balancing intestinal flora, improving immunity and improving oral cavity.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The invention discloses a probiotic with the depression improving effect and a microecological preparation and application thereof, and is characterized in that the probiotic comprises lactobacillus mucus PC17 with the preservation number of CGMCC No.33866, the active ingredients of the lactobacillus microecological preparation comprise thalli of the lactobacillus mucus PC17, and a preparation method of the microecological preparation comprises the following steps: adding the lactobacillus mucus PC17 into the lactobacillus mucus PC17, uniformly mixing the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17 and the lactobacillus mucus PC17 The lactic acid bacteria are inoculated into a fermentation culture medium according to a volume ratio of 1-5% to prepare the lactic acid bacteria microecological preparation, can be used for preparing antidepressant drugs, and has the advantages of being capable of promoting RIN14B cells to secrete 5-hydroxytryptamine and 5-hydroxytryptophan, reducing the levels of interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, corticosterone and corticotropin in serum, and improving the antidepressant activity of the antidepressant drugs. The levels of aminobutyric acid and brain-derived neurotrophic factors in serum are improved, and the depression-like behaviors of CUMS depression model mice are obviously improved.

The invention provides a Lactobacillus paracasei, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.32951. The Lactobacillus paracasei has a preservation number of CGMCC No.32951. The Lactobacillus paracasei has a preservation number of CGMCC No.32951. The lactobacillus paracasei ID001 provided by the invention has an inhibition effect on enteropathogenic bacteria such as salmonella, escherichia coli, staphylococcus aureus and the like. The probiotic preparation is obtained through fermentation and the dendrobium officinale extract, and the probiotic preparation has the immunoregulation effect through performance verification and screening.

The invention discloses a microbial agent and a method for repairing cadmium-polluted rice field soil by using the microbial agent, relates to the technical field of repairing cadmium pollution of rice field soil, and solves the problem that the cadmium metal enrichment capacity of duckweed is limited due to the fact that the duckweed has certain endurance capacity to cadmium stress. The microbial agent comprises bacillus subtilis ATCC6633 and pseudomonas aeruginosa ATCC27853, and the mass ratio of the bacillus subtilis ATCC6633 to the pseudomonas aeruginosa ATCC27853 is 2: 1, and the method comprises the following steps: step 1, spreading the microbial agent on the surface layer of cadmium-polluted rice field soil, then mixing the microbial agent with the rice field soil, raking the rice field soil, adjusting the water-holding capacity of the rice field soil, applying a base fertilizer, and irrigating the rice field. According to the method, the cadmium tolerance of the duckweed can be promoted, so that the enrichment capacity of the duckweed on cadmium is promoted, the remediation capacity of the duckweed on cadmium-polluted soil is improved, the cadmium content in the soil can be reduced, and the content of metal cadmium in rice is also reduced through a competitive cadmium absorption way.

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The present application relates to animal bifidobacterium lactis Ca360 and its application. The present application also provides the application of animal bifidobacterium lactis in promoting the absorption and transport of minerals, improving osteoporosis, iron deficiency anemia and zinc deficiency. The animal bifidobacterium lactis of the present application can promote the absorption and transport of minerals, improve osteoporosis, iron deficiency anemia and zinc deficiency.

The invention discloses a ginseng extract rich in rare saponins and preparation and anti-aging application thereof, and belongs to the technical field of oral beauty and skin care products, the ginseng extract is obtained by fermentation treatment of ginseng powder through phytobacterium plantarum, and the preservation number of the phytobacterium plantarum is GDMCC No.64374. The ginseng extract rich in rare saponin is fermented by specific plant lactobacillus, ginsenoside is efficiently converted into CK and F1, various natural plant components such as ginseng polysaccharide are reserved, and a specific synergistic effect is generated. Through verification, the prepared ginseng extract is high in bioavailability, can more effectively inhibit cell inflammation, and can more effectively recover the mitochondrial function of senescent cells and inhibit cell senescence. The preparation method provided by the invention is high in operability and easy to standardize and intellectualize. Compared with a traditional extraction and purification method, the adopted fermentation method is more environmentally friendly and efficient, and large-scale production can be achieved in industrial production.

The invention relates to the field of microorganisms, and discloses an aspergillus cristatus strain and application thereof. The preservation number of the aspergillus cristatus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.41727. The aspergillus cristatus provided by the invention can produce chymosin at high yield, has excellent curding activity and relatively low proteolytic activity, and can reduce the bitter taste of dairy products and improve the yield; the aspergillus cristatus provided by the invention not only can shorten the fermentation time of the dairy product, improve the cream flavor of the dairy product, reduce the peculiar smell of the dairy product, balance the sourness and sweetness of the dairy product and improve the cinnamon flavor, but also has intestinal colonization ability, and has the potential of improving the flavor level of the dairy product and becoming intestinal probiotics.

The invention discloses pediococcus acidilactici and application thereof, and relates to the technical field of microorganisms. The pediococcus acidilactici is pediococcus acidilactici Mei.1103.1, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.32564, the preservation date is November 11, 2024, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.32564. The pediococcus acidilactici Mei.1103.1 is CGMCC NO.32564. The pediococcus acidilactici Mei.1103.1. The invention also provides application of the fermented dry rice flour in preparation of products for reducing uric acid, preventing / treating hyperuricemia, regulating immunity, preventing / treating kidney injury and preventing / treating fatty liver and fermented dry rice flour. The bacterium can reduce uric acid and relieve hyperuricemia; regulating blood fat; kidney and liver injuries caused by hyperuricemia are repaired; the content of volatile flavor compounds in the fermented dry rice flour is increased.

The invention relates to bifidobacterium animalis subsp. Lactis Ca360 and application thereof. The invention further provides application of the animal bifidobacterium subsp. Lactis in promoting absorption and transport of mineral substances and improving osteoporosis, iron-deficiency anemia and zinc deficiency. The bifidobacterium animalis subsp. Lactis disclosed by the invention can promote absorption and transport of mineral substances and improve osteoporosis, iron-deficiency anemia and zinc deficiency.

The invention belongs to the technical field of microorganisms, and provides streptococcus salivarius subsp. Thermophilus HX-ST36, and the preservation number of the streptococcus salivarius subsp. Thermophilus HX-ST36 is CGMCC NO.32955. The streptococcus salivarius subsp. Thermophilus provided by the invention can be used for fermenting astragalus membranaceus-angelica sinensis. In a cold coagulation and blood stasis type primary dysmenorrheal model mouse, a fermentation product can remarkably inhibit the writhing reaction, and the effects of relieving dysmenorrheal and enriching blood can be achieved by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissue and increasing the content of hemoglobin (HGB). The dampness clearing effect of the fermentation product is verified by improving the heavy physical sign of moisture through crowd experiments. The invention also provides a preparation method of the probiotic preparation and the probiotic fermentation product. The preparation methods of the probiotic preparation and the probiotic fermentation product are simple, and large-scale production can be realized.

The invention relates to bifidobacterium animalis subsp. Lactis C-2 with an effect of improving inflammatory bowel diseases and application of the bifidobacterium animalis subsp. Lactis C-2. The bifidobacterium animalis subsp. Lactis C-2 is classified and named as Bifidobacterium animalis subsp. Lactis, and the preservation number of the bifidobacterium animalis subsp. Lactis C-2 is GDMCC No: 65455. The bacterial strain can inhibit the colonization of harmful bacteria and promote the colonization of beneficial bacteria and optimize the composition of intestinal microorganisms by regulating the intestinal microecology, repairing the intestinal mechanical barrier, improving the intestinal immune response, inhibiting oxidative stress reaction and other action mechanisms, thereby inducing anti-inflammatory response and improving the intestinal barrier function. Therefore, the bifidobacterium animalis subsp. Lactis C-2 strain is suitable for preparing the medicine with the effect of preventing, improving or treating the inflammatory bowel disease.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The application provides a lactobacillus rhamnosus GS044 and a lyophilized powder and a probiotic of the lactobacillus rhamnosus GS044 in application to promoting growth and development and improving immunity, and belongs to the technical field of microorganisms.The application provides a lactobacillus rhamnosus GS044 with a preservation number of CGMCC No.33713.The application further provides a method for preparing gamma-aminobutyric acid by using the lactobacillus rhamnosus GS044, and a lyophilized powder prepared by using the lactobacillus rhamnosus GS044, a probiotic and application of the lyophilized powder and the probiotic.The lyophilized powder and the probiotic prepared by the application can effectively promote growth and development and improve immunity.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The invention relates to a bifidobacterium longum subsp. Infantis Imu-01 and an application thereof. Specifically, the invention provides bifidobacterium longum subsp.infantis Imu-01, a culture, a preparation and application of the bifidobacterium longum subsp.infantis Imu-01 in preparation of a product for improving the immune function. The bifidobacterium longum subsp. Infantis provided by the invention can regulate and control the immune level of an object, improve the content of butyric acid in intestinal tracts and improve the utilization of oligosaccharide in breast milk.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The present disclosure provides agronomically stable liquid agricultural compositions, methods of formulation thereof, and methods of application thereof. The agricultural compositions comprise nitrogen-fixing microorganisms and one or more of a buffering agent, a microbial stabilizer, and a physical stabilizer. The disclosed liquid agricultural compositions have a longer shelf life and greater ease of application than other existing dry and liquid formulations. The disclosed liquid agricultural compositions are stable for a period of thirty days or longer with low toxin accumulation and high microbial stability. The compositions are suitable for use on agricultural plant tissues or the environs thereof for providing a source of fixed atmospheric nitrogen to the agricultural plant. The compositions are used to increase crop yield and decrease yield variance.

The invention belongs to the field of fermentation, and particularly relates to a complex microbial inoculant, original cheese, processed cheese with a blood sugar reducing function and preparation and application of the complex microbial inoculant. Wherein the processed cheese is prepared by the method comprising the following steps: mixing the components including raw cheese, sugarcane juice, corn oil, casein, concentrated milk protein, blueberry powder, mono / diglycerol fatty acid ester, sodium citrate, sodium tripolyphosphate, sodium hexametaphosphate, salt guar gum, potassium sorbate and water, and then emulsifying to obtain the processed cheese, the sugar cane juice, the corn oil, the casein, the concentrated milk protein, the blueberry powder, the mono / diglycerol fatty acid ester, the sodium citrate, the sodium tripolyphosphate, the sodium hexametaphosphate, the salt guar gum, the potassium sorbate and the water, the original cheese is prepared from a complex microbial inoculant, and the complex microbial inoculant comprises lactococcus lactis bacterial powder, lactococcus lactis subsp. Lactis bacterial powder, lactobacillus delbrueckii subsp. Lactis bacterial powder, lactobacillus plantarum bacterial powder and lactobacillus paracasei bacterial powder. The processed cheese provided by the invention has a better function of controlling blood sugar, and meanwhile, the processed cheese is fine and uniform in texture and has good smearing performance.

The invention discloses an application of Lactobacillus plantarum in at least one of the following items: a1, an application in improving the silage quality of silage, a2, an application in improving the silage quality, a3, an application in improving the silage quality, a4, an application in improving the silage quality, and a5, an application in improving the silage quality. A2, application in preparation of silage; a3, application in preparation of a silage additive; the strain name of the lactobacillus plantarum is LA2, the lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus plantarum is CGMCC No. 33484. According to the invention, the lactobacillus plantarum or the microbial inoculum thereof or the silage additive thereof is added into the silage for the first time, and tetracycline resistance genes in the silage can be remarkably reduced; after the lactobacillus plantarum or the microbial inoculum thereof or the silage additive thereof disclosed by the invention is added, the generation and residue of tetracycline resistance genes in feed can be reduced, the propagation of the tetracycline resistance genes in a food chain is reduced, the safety of forage grass is ensured, and the damage to animals, environment and human bodies is reduced; and the drug resistance of human pathogenic bacteria is reduced while the health level of animals is improved.

The invention provides a complex microbial inoculant as well as a preparation method and application thereof, and relates to the technical field of complex microbial inoculant preparation. The compound bacterial agent is prepared from bacillus pumilus JMB004, bacillus safeensis strain BXC22 and bacillus sp. EB35, and the compound bacterial agent is prepared from bacillus pumilus strain JMB004, bacillus safeensis strain BXC22 and bacillus sp. EB35. The three strains are obtained through autonomous screening and prepared into the complex microbial inoculant, dry powder can be stored at normal temperature, the storage life is prolonged to one year, and the composite microbial inoculant is convenient to carry. The complex microbial inoculant is only composed of three strains of bacteria, and the components are simple; the low-temperature composting agent is applied to composting treatment under a low-temperature condition, and is relatively high in temperature rise rate, relatively short in fermentation time and relatively high in decomposition degree.

The invention relates to the technical field of soil remediation, in particular to a charcoal-fungus symbiont for a freeze-thaw environment and a preparation method and application of the charcoal-fungus symbiont. The preparation method of the charcoal-fungus symbiont comprises the following steps: crushing a biomass raw material, and carrying out pyrolysis to prepare basic charcoal; placing the basic biochar in an iron oxide solution, and carrying out an iron oxide loading reaction to obtain iron oxide biochar; the preparation method comprises the following steps: dipping iron oxide biochar in a chitosan solution for coating modification, then continuously adding a cross-linking agent for cross-linking solidification, and freeze-drying to obtain chitosan-iron oxide composite modified biochar; and immersing the chitosan-iron oxide composite modified biochar into a bacterial suspension of functional microorganisms, oscillating and adsorbing, and freeze-drying to obtain the carbon-bacteria symbiont. The invention aims to improve the heavy metal pollution phenomenon of soil and the soybean root rot disaster in the freezing and thawing cycle environment at the same time.

According to the microbial flora culture method for efficiently degrading the organic pollutants, three continuous culture stages of enrichment, domestication and optimization are adopted, parameters such as the temperature, the pH value, the rotating speed and the ventilatory capacity are accurately controlled in each stage, and growth of microorganisms with degradation capacity is promoted. Through multi-stage culture and domestication, the obtained flora has efficient degradation capability on organic pollutants such as petroleum hydrocarbons, pesticides and polycyclic aromatic hydrocarbons, and has strong adaptability to pollutants with different concentrations. The 16S rRNA gene is used for sequencing and identifying the flora, and composition and dominant strains are clear. The flora is prepared into a microbial inoculum, and the microbial inoculum is preserved by freeze drying or liquid nitrogen, so that the activity can be effectively maintained. The flora can be widely applied to the fields of polluted soil and water body remediation, industrial wastewater treatment and the like, is green and environment-friendly, is relatively low in cost, avoids secondary pollution, conforms to the concept of sustainable development, and has relatively high economic and environmental values.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention relates to the technical field of biology, in particular to a sample preservation solution for vagina micro-ecology detection and a preparation method of the sample preservation solution. The invention relates to a sample preservation solution for vaginal micro-ecology detection. The sample preservation solution comprises the following components in percentage by mass: 0.5-0.9% of paraformaldehyde, 0.08-0.12% of glutaraldehyde, 10-18% of a buffer solution, 0.1-0.4% of inositol, 1-10% of a stabilizer, 0.8-5% of a cell treatment agent and the balance of water, the stabilizing agent is prepared from sodium alginate, tween-20 and beta-cyclodextrin. The invention aims to solve the problems of high false negative and false positive rate or difficulty in interpretation caused by liquid-based cell accumulation, unstable subsequent dyeing and decoloration time and the like of a cell preservation solution in the prior art, so that the sample preservation solution for sample cells can keep the forms of cells, protozoa and the like unchanged, and ensures that gram staining negative and positive properties of bacteria and fungi are not changed. The staining and decoloring are relatively stable, the false negative and false positive rate of sample cell detection is effectively reduced, and the sample cell lesion detection and judgment are more accurate.

The invention provides a spleen and stomach conditioning factor lactobacillus reuteri obtained by fermenting poria cocos powder. The strain has good acid resistance, cholate resistance and antibacterial ability. The invention further provides a probiotic preparation which has an obvious effect on improving the spleen and the stomach. In addition, the invention also provides a composite preparation composition containing Lactobacillus reuteri, and the composition effectively promotes normal peristalsis and digestion and absorption functions of gastrointestinal tracts by regulating the secretion level of gastrointestinal hormones, enhances the immunity of the organism, protects intestinal mucosa barriers, comprehensively improves the symptoms of weakness of the spleen and the stomach, and improves the overall health level.

The invention provides a microecological preparation with an immunological enhancement function and application of the microecological preparation in antibiotic-free breeding of livestock and poultry, and relates to the technical field of agricultural biology. The microecological preparation with the immunological enhancement function comprises a probiotic component, a prebiotics component and an immunological enhancement component, and the probiotic component, the prebiotics component and the immunological enhancement component are respectively composed of lactic acid bacteria, bacillus, clostridium butyricum, fructo-oligosaccharide, yeast beta-glucan, astragalus polysaccharide and chitosan oligosaccharide. Wherein the lactic acid bacteria accounts for 30%-40%, the bacillus accounts for 20%-30%, the clostridium butyricum accounts for 10%-20%, the fructo-oligosaccharide accounts for 5%-10%, the yeast beta-glucan accounts for 3%-8%, the astragalus polysaccharide accounts for 2%-5%, and the chitosan The probiotics can enhance immunity and regulate intestinal tracts at the same time, the effect is improved by 40% or above compared with common probiotics, animals suffer less illness and grow fast, flora can resist high temperature and gastric acid through a special protection technology, the loss of viable bacteria is less than 5% after the probiotics are stored for 3 months, and the probiotics are stable by two times compared with conventional products.