2342results about "Microorganism preservation" patented technology

The present disclosure provides water-soluble film packages composed of a water-soluble film, where the packages include: one or more compartment(s) including dehydrated microbes. The disclosure further provides methods of production of the water-soluble film packages, and methods of application thereof. The water-soluble packages of the disclosure enable convenient and accurate application of microbial dry powders to plants, promote uniform dispersion of powdered microbes, enhance microbial shelf life, and enhance the survival of microbes on seed, while being environmentally sustainable.

The invention provides a bifidobacterium animalis subsp. Lactis strain B21247 with a hypoglycemic function and application thereof, and belongs to the technical field of functional microorganisms. The invention relates to a bifidobacterium animalis subsp. Lactis strain B21247 with a hypoglycemic function. The preservation number of the bifidobacterium animalis subsp. Lactis strain B21247 is GDMCC No: 65075. The strain B21247 has a good intestinal colonization function, and has the activity of inhibiting alpha-glucosidase in vitro. Meanwhile, a type 2 diabetes mouse model is used as an experimental model to verify that the strain B21247 achieves the effect of reducing blood sugar by adjusting glycosylated hemoglobin, reducing pancreatic beta cell damage and increasing secretion of intestinal GLP-1, and a new thought is provided for reducing blood sugar or treating diabetes.

The invention relates to lactobacillus rhamnosus CBBM005 and application thereof in products for balancing intestinal flora, improving immunity and improving oral cavity, and belongs to the technical field of microorganisms. The lactobacillus rhamnosus CBBM005 is separated from human intestinal secretion, the lactobacillus rhamnosus CBBM005 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation time is March 20, 2025, and the preservation number is CGMCC NO.33904. The lactobacillus rhamnosus CBBM005 has the characteristics of balancing intestinal flora, improving immunity and improving oral cavity.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The invention discloses a probiotic with the depression improving effect and a microecological preparation and application thereof, and is characterized in that the probiotic comprises lactobacillus mucus PC17 with the preservation number of CGMCC No.33866, the active ingredients of the lactobacillus microecological preparation comprise thalli of the lactobacillus mucus PC17, and a preparation method of the microecological preparation comprises the following steps: adding the lactobacillus mucus PC17 into the lactobacillus mucus PC17, uniformly mixing the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17 and the lactobacillus mucus PC17 The lactic acid bacteria are inoculated into a fermentation culture medium according to a volume ratio of 1-5% to prepare the lactic acid bacteria microecological preparation, can be used for preparing antidepressant drugs, and has the advantages of being capable of promoting RIN14B cells to secrete 5-hydroxytryptamine and 5-hydroxytryptophan, reducing the levels of interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, corticosterone and corticotropin in serum, and improving the antidepressant activity of the antidepressant drugs. The levels of aminobutyric acid and brain-derived neurotrophic factors in serum are improved, and the depression-like behaviors of CUMS depression model mice are obviously improved.

The invention provides lactobacillus plantarum NSL0125 and application thereof in preparation of a microbial preparation for promoting calcium supplement and growth and development. The lactobacillus plantarum NSL0125 is classified and named as Lactobacillus plantarum and is preserved in the China Center for Type Culture Collection, and the preservation number of the lactobacillus plantarum NSL0125 is CCTCC (China Center for Type Culture Collection) NO: M 2024609. The lactobacillus plantarum NSL0125 is high in safety, free of pathogenicity and capable of fermenting various nutrient substances to supply energy for growth. Experiments prove that the lactobacillus plantarum NSL0125 can be colonized in intestinal tracts for a long time, and meanwhile, the lactobacillus plantarum NSL0125 has unique effects on the aspects of adsorption, transfer, absorption promotion and the like of calcium ions, so that calcium absorption, growth and development of organisms are effectively promoted. The lactobacillus plantarum NSL0125 is high in fermentation level and suitable for large-scale industrial production. A large number of experimental studies prove that the lactobacillus plantarum NSL0125 and the microbial preparation thereof have remarkable effects of promoting calcium supplement and growth and development, have good application value and have good market application prospects.

The invention provides a Lactobacillus paracasei, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.32951. The Lactobacillus paracasei has a preservation number of CGMCC No.32951. The Lactobacillus paracasei has a preservation number of CGMCC No.32951. The lactobacillus paracasei ID001 provided by the invention has an inhibition effect on enteropathogenic bacteria such as salmonella, escherichia coli, staphylococcus aureus and the like. The probiotic preparation is obtained through fermentation and the dendrobium officinale extract, and the probiotic preparation has the immunoregulation effect through performance verification and screening.

The invention discloses a microbial agent and a method for repairing cadmium-polluted rice field soil by using the microbial agent, relates to the technical field of repairing cadmium pollution of rice field soil, and solves the problem that the cadmium metal enrichment capacity of duckweed is limited due to the fact that the duckweed has certain endurance capacity to cadmium stress. The microbial agent comprises bacillus subtilis ATCC6633 and pseudomonas aeruginosa ATCC27853, and the mass ratio of the bacillus subtilis ATCC6633 to the pseudomonas aeruginosa ATCC27853 is 2: 1, and the method comprises the following steps: step 1, spreading the microbial agent on the surface layer of cadmium-polluted rice field soil, then mixing the microbial agent with the rice field soil, raking the rice field soil, adjusting the water-holding capacity of the rice field soil, applying a base fertilizer, and irrigating the rice field. According to the method, the cadmium tolerance of the duckweed can be promoted, so that the enrichment capacity of the duckweed on cadmium is promoted, the remediation capacity of the duckweed on cadmium-polluted soil is improved, the cadmium content in the soil can be reduced, and the content of metal cadmium in rice is also reduced through a competitive cadmium absorption way.

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The present application relates to animal bifidobacterium lactis Ca360 and its application. The present application also provides the application of animal bifidobacterium lactis in promoting the absorption and transport of minerals, improving osteoporosis, iron deficiency anemia and zinc deficiency. The animal bifidobacterium lactis of the present application can promote the absorption and transport of minerals, improve osteoporosis, iron deficiency anemia and zinc deficiency.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum 9A-2 and application thereof in preparation of products for regulating intestinal tracts and protecting eyes. A probiotic product is successfully researched and developed on the basis of the lactobacillus plantarum, and verification shows that the lactobacillus plantarum can continuously provide endophytic essential isorhamnetin, chromium and vitamin H in the intestinal reproduction and growth process while regulating intestinal flora balance and promoting digestion, and also has the effect of relieving visual fatigue; particularly, visual fatigue caused by eye related diseases is relieved. The endophytic essential quantity of isorhamnetin, chromium and vitamin H can be continuously provided in the intestinal reproduction and growth process by feeding 3 billion strains and 200 milligrams of metabolites of the strain every day, visual fatigue caused by eye related diseases can be prevented and relieved, and the body health can be kept.

The invention relates to the technical field of biological control, in particular to bacillus velezensis JNJK-1, a microbial agent and application of the bacillus velezensis JNJK-1 and the microbial agent in edible fungus disease control, the bacillus velezensis JNJK-1 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CMCC No.31075. The novel bacillus velezensis JNJK-1 is screened out for the first time, and the bacillus velezensis can be applied to biological control of edible mushroom diseases and has a broad-spectrum inhibition effect on pathogenic bacteria of edible mushrooms. Meanwhile, the inventor also finds that the bacillus velezensis JNJK-1, curcumin and sodium alginate are compounded into the microbial agent, so that the inhibition effect on the pathogenic bacteria of the edible fungi can be improved, and the microbial activity can be maintained.

The invention discloses a ginseng extract rich in rare saponins and preparation and anti-aging application thereof, and belongs to the technical field of oral beauty and skin care products, the ginseng extract is obtained by fermentation treatment of ginseng powder through phytobacterium plantarum, and the preservation number of the phytobacterium plantarum is GDMCC No.64374. The ginseng extract rich in rare saponin is fermented by specific plant lactobacillus, ginsenoside is efficiently converted into CK and F1, various natural plant components such as ginseng polysaccharide are reserved, and a specific synergistic effect is generated. Through verification, the prepared ginseng extract is high in bioavailability, can more effectively inhibit cell inflammation, and can more effectively recover the mitochondrial function of senescent cells and inhibit cell senescence. The preparation method provided by the invention is high in operability and easy to standardize and intellectualize. Compared with a traditional extraction and purification method, the adopted fermentation method is more environmentally friendly and efficient, and large-scale production can be achieved in industrial production.

The invention relates to the field of microorganisms, and discloses an aspergillus cristatus strain and application thereof. The preservation number of the aspergillus cristatus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.41727. The aspergillus cristatus provided by the invention can produce chymosin at high yield, has excellent curding activity and relatively low proteolytic activity, and can reduce the bitter taste of dairy products and improve the yield; the aspergillus cristatus provided by the invention not only can shorten the fermentation time of the dairy product, improve the cream flavor of the dairy product, reduce the peculiar smell of the dairy product, balance the sourness and sweetness of the dairy product and improve the cinnamon flavor, but also has intestinal colonization ability, and has the potential of improving the flavor level of the dairy product and becoming intestinal probiotics.

The invention discloses pediococcus acidilactici and application thereof, and relates to the technical field of microorganisms. The pediococcus acidilactici is pediococcus acidilactici Mei.1103.1, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.32564, the preservation date is November 11, 2024, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.32564. The pediococcus acidilactici Mei.1103.1 is CGMCC NO.32564. The pediococcus acidilactici Mei.1103.1. The invention also provides application of the fermented dry rice flour in preparation of products for reducing uric acid, preventing / treating hyperuricemia, regulating immunity, preventing / treating kidney injury and preventing / treating fatty liver and fermented dry rice flour. The bacterium can reduce uric acid and relieve hyperuricemia; regulating blood fat; kidney and liver injuries caused by hyperuricemia are repaired; the content of volatile flavor compounds in the fermented dry rice flour is increased.

The invention discloses pediococcus pentosaceus CCFM1402 capable of effectively repairing a skin barrier and a self-fermentation metabolite of the pediococcus pentosaceus CCFM1402, and belongs to the technical field of microorganisms and medicines. The pediococcus pentosaceus CCFM1402 provided by the invention has the effect of improving the skin barrier function in both external use and oral administration; the cell activity of damaged HaCaT cells and the expression of the gene level of skin barrier function protein and tight junction protein can be improved by virtue of a thallus lysate and a fermented supernatant (metagen) of the external pediococcus pentosaceus CCFM1402; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of appearance, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the pediococcus pentosaceus CCFM1402. Therefore, the pediococcus pentosaceus CCFM1402 has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention relates to bifidobacterium animalis subsp. Lactis Ca360 and application thereof. The invention further provides application of the animal bifidobacterium subsp. Lactis in promoting absorption and transport of mineral substances and improving osteoporosis, iron-deficiency anemia and zinc deficiency. The bifidobacterium animalis subsp. Lactis disclosed by the invention can promote absorption and transport of mineral substances and improve osteoporosis, iron-deficiency anemia and zinc deficiency.

The invention discloses lactobacillus reuteri CCFM1288 for relieving depressive mood and application of the lactobacillus reuteri CCFM1288, and belongs to the technical field of microorganisms. The lactobacillus reuteri CCFM1288 has the tryptophan metabolism capability, and can survive for 7 days in the intestinal tract; after the lactobacillus reuteri CCFM1288 intervenes a depressive mouse, anxiety-like and depression-like behaviors of the mouse can be obviously relieved, flora alpha-diversity is improved, intestinal symbiotic bacteria are promoted to secrete indole substances, intestinal homeostasis is maintained, hypothalamic-pituitary-adrenal axis hyperfunction is relieved, dopamine and serotonin neurotransmitter nervous system functions are improved, and the effect of preventing and treating depression is achieved. The neurotransmitter release is promoted, and the anti-depression effect is achieved. In clinical tests, intervention of Lactobacillus reuteri CCFM1288 can significantly improve the depressive symptom of MDD patients, and a new safe alleviation approach is provided for depression treatment.

The invention belongs to the technical field of microorganisms, and provides streptococcus salivarius subsp. Thermophilus HX-ST36, and the preservation number of the streptococcus salivarius subsp. Thermophilus HX-ST36 is CGMCC NO.32955. The streptococcus salivarius subsp. Thermophilus provided by the invention can be used for fermenting astragalus membranaceus-angelica sinensis. In a cold coagulation and blood stasis type primary dysmenorrheal model mouse, a fermentation product can remarkably inhibit the writhing reaction, and the effects of relieving dysmenorrheal and enriching blood can be achieved by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissue and increasing the content of hemoglobin (HGB). The dampness clearing effect of the fermentation product is verified by improving the heavy physical sign of moisture through crowd experiments. The invention also provides a preparation method of the probiotic preparation and the probiotic fermentation product. The preparation methods of the probiotic preparation and the probiotic fermentation product are simple, and large-scale production can be realized.

The invention relates to bifidobacterium animalis subsp. Lactis C-2 with an effect of improving inflammatory bowel diseases and application of the bifidobacterium animalis subsp. Lactis C-2. The bifidobacterium animalis subsp. Lactis C-2 is classified and named as Bifidobacterium animalis subsp. Lactis, and the preservation number of the bifidobacterium animalis subsp. Lactis C-2 is GDMCC No: 65455. The bacterial strain can inhibit the colonization of harmful bacteria and promote the colonization of beneficial bacteria and optimize the composition of intestinal microorganisms by regulating the intestinal microecology, repairing the intestinal mechanical barrier, improving the intestinal immune response, inhibiting oxidative stress reaction and other action mechanisms, thereby inducing anti-inflammatory response and improving the intestinal barrier function. Therefore, the bifidobacterium animalis subsp. Lactis C-2 strain is suitable for preparing the medicine with the effect of preventing, improving or treating the inflammatory bowel disease.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The application provides a lactobacillus rhamnosus GS044 and a lyophilized powder and a probiotic of the lactobacillus rhamnosus GS044 in application to promoting growth and development and improving immunity, and belongs to the technical field of microorganisms.The application provides a lactobacillus rhamnosus GS044 with a preservation number of CGMCC No.33713.The application further provides a method for preparing gamma-aminobutyric acid by using the lactobacillus rhamnosus GS044, and a lyophilized powder prepared by using the lactobacillus rhamnosus GS044, a probiotic and application of the lyophilized powder and the probiotic.The lyophilized powder and the probiotic prepared by the application can effectively promote growth and development and improve immunity.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The present invention relates to compositions comprising a microbial consortium for treating or preventing medical disorders and pathologies through microbiome modulation, including medical disorders associated with a dysbiosis or a loss of gut microbial function.

The invention belongs to the technical field of biological fermentation, and relates to a high-bacteriostatic-activity propionibacterium scherichia subspecies and application thereof, in particular to a propionibacterium scherichia subspecies which is classified and named as Propionibacterium fredenreichiii subsp. Scherichia CJPF0001, and the preservation number of the propionibacterium scherichia subspecies is CCTCC (China Center for Type Culture Collection) No.M 20242477. The invention further relates to a preparation method of the propionibacterium scherichia subspecies and application of the propionibacterium scherichia subspecies. The fermentation product of the strain has high protein and high bacteriostatic activity, can inhibit the growth of common spoilage microorganisms, and has the effects of improving the nutritional value of food and prolonging the shelf life of food and the like.

The invention discloses parabacteroides faecium capable of relieving senescence and application of the parabacteroides faecium, and belongs to the technical field of microorganisms and the technical field of medicines. Animal experiments prove that Parabacteroides vervae CCFM1428 has the effects of improving the weakness index of a mouse, increasing peroxide dismutase in the liver of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px), increasing the level of catalase, reducing an inflammatory factor IL-6, reducing an inflammatory factor TNF-alpha and increasing the level of acetylcholine in the brain of the mouse, and has the effects of improving the weakness index of the mouse, increasing the liver peroxide dismutase of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px) and the level of catalase. The gripping force of the mouse is improved. The strain disclosed by the invention has a huge application prospect in preparation of products for relieving senescence.

The invention relates to a bifidobacterium longum subsp. Infantis Imu-01 and an application thereof. Specifically, the invention provides bifidobacterium longum subsp.infantis Imu-01, a culture, a preparation and application of the bifidobacterium longum subsp.infantis Imu-01 in preparation of a product for improving the immune function. The bifidobacterium longum subsp. Infantis provided by the invention can regulate and control the immune level of an object, improve the content of butyric acid in intestinal tracts and improve the utilization of oligosaccharide in breast milk.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The invention discloses a lactobacillus plantarum strain and application thereof, the lactobacillus plantarum strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 29, 2024, and the preservation number is CGMCC NO.31485. The lactobacillus plantarum strain is named as lactobacillus plantarum, and the lactobacillus plantarum strain is named as lactobacillus plantarum. The lactobacillus plantarum C0502 can effectively realize degradation of xylan, not only provides a new way for realizing high-value utilization of xylan, but also can promote growth and metabolism of lactic acid bacteria, so that the lactobacillus plantarum C0502 can greatly reduce the production cost of probiotics and metabolites thereof, and has great application value and economic benefit.