874results about "Microorganism preservation" patented technology

The present application relates to animal bifidobacterium lactis Ca360 and its application. The present application also provides the application of animal bifidobacterium lactis in promoting the absorption and transport of minerals, improving osteoporosis, iron deficiency anemia and zinc deficiency. The animal bifidobacterium lactis of the present application can promote the absorption and transport of minerals, improve osteoporosis, iron deficiency anemia and zinc deficiency.

The invention relates to the field of microorganisms, and discloses an aspergillus cristatus strain and application thereof. The preservation number of the aspergillus cristatus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.41727. The aspergillus cristatus provided by the invention can produce chymosin at high yield, has excellent curding activity and relatively low proteolytic activity, and can reduce the bitter taste of dairy products and improve the yield; the aspergillus cristatus provided by the invention not only can shorten the fermentation time of the dairy product, improve the cream flavor of the dairy product, reduce the peculiar smell of the dairy product, balance the sourness and sweetness of the dairy product and improve the cinnamon flavor, but also has intestinal colonization ability, and has the potential of improving the flavor level of the dairy product and becoming intestinal probiotics.

The present disclosure provides agronomically stable liquid agricultural compositions, methods of formulation thereof, and methods of application thereof. The agricultural compositions comprise nitrogen-fixing microorganisms and one or more of a buffering agent, a microbial stabilizer, and a physical stabilizer. The disclosed liquid agricultural compositions have a longer shelf life and greater ease of application than other existing dry and liquid formulations. The disclosed liquid agricultural compositions are stable for a period of thirty days or longer with low toxin accumulation and high microbial stability. The compositions are suitable for use on agricultural plant tissues or the environs thereof for providing a source of fixed atmospheric nitrogen to the agricultural plant. The compositions are used to increase crop yield and decrease yield variance.

The invention relates to the technical field of soil remediation, in particular to a charcoal-fungus symbiont for a freeze-thaw environment and a preparation method and application of the charcoal-fungus symbiont. The preparation method of the charcoal-fungus symbiont comprises the following steps: crushing a biomass raw material, and carrying out pyrolysis to prepare basic charcoal; placing the basic biochar in an iron oxide solution, and carrying out an iron oxide loading reaction to obtain iron oxide biochar; the preparation method comprises the following steps: dipping iron oxide biochar in a chitosan solution for coating modification, then continuously adding a cross-linking agent for cross-linking solidification, and freeze-drying to obtain chitosan-iron oxide composite modified biochar; and immersing the chitosan-iron oxide composite modified biochar into a bacterial suspension of functional microorganisms, oscillating and adsorbing, and freeze-drying to obtain the carbon-bacteria symbiont. The invention aims to improve the heavy metal pollution phenomenon of soil and the soybean root rot disaster in the freezing and thawing cycle environment at the same time.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus agave, fermentation liquor, a biological agent and application. The invention discloses a strain of bacillus tequilensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus tequilensis is CGMCC NO.34830. The strain of bacillus tequilensis has the advantages that the preservation number is CGMCC NO.34830; the invention also discloses a fermentation broth and a biological agent prepared according to the bacillus agave, and the bacillus agave, the fermentation broth and the biological agent provided by the invention can effectively reduce the harm of root knot nematode disease to plants and promote seed germination or crop growth, and have the advantages of high efficiency, safety, environmental protection and difficult generation of resistance.

The invention relates to the technical field of agricultural microorganisms. The invention provides a compound microorganism bacterium suspension, a bacterium agent, a tobacco seed coating agent as well as a preparation method and application of the compound microorganism bacterium suspension. The compound microorganism bacterium suspension comprises a rice seed endophytic bacterium spiraceae R3 bacterium suspension and a rice seed endophytic bacterium stenotrophomonas R5 bacterium suspension; the mass ratio of R3 to R5 is 1: (0.5-1.5); the endophytic bacterium herbapiril R3 of the rice seeds is Herbapiril sp. R3, and the preservation number of the herbapiril R3 is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the rice seed endophytic bacterium stenotrophomonas R5 is Shenotrophomonas sp. R5, and the preservation number of the rice seed endophytic bacterium stenotrophomonas R5 is CCTCC (China Center for Type Culture Collection) NO: M 20231758. The compound microbial suspension is used as a seed coating agent to coat tobacco seeds, and growth of tobacco root systems can be remarkably promoted.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention discloses an Akkermansia massiliensis LTA21F2 strain and an application of the Akkermansia massiliensis LTA21F2 strain, and belongs to the technical field of microorganisms. The strain is preserved in the Guangdong Microbial Culture Collection Center on December 04, 2022, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 63035. The Akkermansia massiliensis anaerobic fermentation bacterial liquid provided by the invention is high in viable count and strong in oxygen tolerance, still has a survival rate of more than 90% after being exposed in air for 12 hours, the viable count in a sample is increased by 25.26% after being stressed by 1.5 M H2O2 for 2 hours, and the strain has a section of characteristic sequence which can be used as a specific molecular marker, and has the characteristics of high growth rate, high viable count and the like when being subjected to enlarged culture in a 200L fermentation tank.

The present application relates to a kind of Bifidobacterium longum infantis subsp.Imu-01 and its application. Specifically, the present application provides Bifidobacterium longum infantis subsp.Imu-01, culture, preparation and preparation of immune function improving product application. The present application provides that Bifidobacterium longum infantis can regulate the immune level of the subject, improve the content of intestinal butyric acid, improve the utilization of human milk oligosaccharide.

The invention relates to lactobacillus gasseri FMTA1121E with an effect of regulating a vaginal micro-ecological environment and application of the lactobacillus gasseri FMTA1121E. The lactobacillus gasseri FMTA1121E is classified and named as lactobacillus gasseri, and the preservation number of the lactobacillus gasseri FMTA1121E is CGMCC No.34962. The lactobacillus gasseri FMTA1121E can be metabolized to generate beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide so as to regulate the genital tract environment and improve the health degree; and the bacillus subtilis has excellent bacteriostasis and sterilization effects on various common vaginal pathogenic bacteria such as escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans, neisseria gonorrhoeae and the like. Therefore, the lactobacillus gasseri FMTA1121E strain can be applied to preparation of preparations for preventing and treating related genital tract diseases such as vaginitis.

The invention relates to the technical field of dairy product fermentation, and discloses a high-activity probiotic microcapsule suitable for dairy product fermentation and a preparation method thereof.The microcapsule is prepared from, by weight, 6.7-13.3 parts of bifidobacterium lactis, 3.3-6.7 parts of lactobacillus plantarum, 9.1-15.2 parts of Arabic gum, 13.6-22.7 parts of maltodextrin, 7.3-12.1 parts of chitosan, 15.1-30.2 parts of trehalose, 3.8-7.5 parts of glycerinum and 1.1-2.3 parts of ascorbic acid. The molecular weight of the chitosan is 80kDa, and the weight part ratio of the bifidobacterium lactis to the lactobacillus plantarum is 2: 1. In the invention, an inner core is formed through a eutectic structure of bifidobacterium lactis, lactobacillus plantarum and trehalose to protect the probiotics, so that the problems that the existing probiotics are easily influenced by the external environment in the storage and fermentation processes, the activity is reduced, and the fermentation effect of dairy products is poor are solved.

The invention discloses a freeze-drying protective agent composition, a freeze-drying protective agent, application of the freeze-drying protective agent, a probiotic freeze-dried product and a preparation method of the probiotic freeze-dried product. The freeze-drying protective agent composition comprises the following components in parts by weight: 0.5-4 parts of tremella polysaccharide; the relative molecular weight of the tremella polysaccharide is 1 KDa to 10 KDa; 0.5 to 4 parts of disaccharide; and 0.5-2 parts of sugar alcohol. The freeze-drying protective agent composition and water are uniformly mixed and then sterilized to obtain the freeze-drying protective agent, and the freeze-drying protective agent is used for preparing probiotic freeze-dried products and can effectively ensure the activity and stability of probiotics.

The present disclosure provides agronomically stable liquid agricultural compositions, methods of formulation thereof, and methods of application thereof. The agricultural compositions comprise nitrogen-fixing microorganisms and one or more of a buffering agent, a microbial stabilizer, and a physical stabilizer. The disclosed liquid agricultural compositions have a longer shelf life and greater ease of application than other existing dry and liquid formulations. The disclosed liquid agricultural compositions are stable for a period of thirty days or longer with low toxin accumulation and high microbial stability. The compositions are suitable for use on agricultural plant tissues or the environs thereof for providing a source of fixed atmospheric nitrogen to the agricultural plant. The compositions are used to increase crop yield and decrease yield variance.

A Bifidobacterium animalis subsp. lactis for reducing Abeta42 deposition and an application, a name of the Bifidobacterium animalis subsp. lactis is Bifidobacterium animalis subsp. lactis IOB-LO7, and classified as Bifidobacterium animalis subsp. lactis, the Bifidobacterium animalis subsp. lactis IOB-LO7 is preserved in the General Microbiology Center of the China General Microbiological Culture Collection Center (CGMCC) on Dec. 23, 2021, with the collection number of CGMCC No. 24185. By reducing the levels of Aβ42 in the cerebral cortex and hippocampus, clearing Aβ amyloid plaques, improving communication between neurons, reducing brain neuroinflammation, and protecting nerve cells, it helps to restore cognitive function and improve Alzheimer's disease. Additionally, it can also improve gut microbiota imbalance caused by Alzheimer's disease.

The invention discloses a probiotic chitosan microsphere and a preparation method thereof, and belongs to the technical field of probiotic embedding, and the method comprises the following steps: dissolving trehalose and L-glutamine in water, suspending lactic acid bacteria in the water, mixing with a chitosan solution to obtain a mixed solution, dropwise adding the mixed solution into a sodium tripolyphosphate solution, and stirring to obtain the probiotic chitosan microsphere. And washing insoluble substances, and performing freeze drying to obtain the probiotic chitosan microspheres. The trehalose and the L-glutamine are used as protective agents and nutrient substances of the probiotics at the same time, can synergistically improve the survival rate of the probiotics, are matched with other components, comprehensively improve the tolerance and activity of the probiotics, and are combined with a specific curing means and freeze drying, so that damage of high temperature to the probiotics can be avoided; the obtained microspheres have good probiotic protection capability, stability and appropriate release performance, can effectively improve the survival rate of the probiotics in storage and application processes, and can better play roles in improving intestinal flora and improving the health level of hosts.

The invention discloses low-GI probiotic compound coarse cereal meal replacement powder and a preparation method thereof. The preparation method comprises the steps of raw material and auxiliary material treatment, starch enzymolysis, strain activation culture, fermentation, freeze drying, blending and packaging and the like. The black tartary buckwheat, the black beans, the black sesame, the black rice and the black mulberries are used as main raw materials, good growth conditions are created for lactic acid bacteria by means of an amylase enzymolysis technology, and further the cereals are subjected to synergistic fermentation by inoculating a strain suspension of lactobacillus reuteri and lactobacillus rhamnosus, so that the nutritional value is increased, bioactive metabolites are added, and the nutritional value is increased. And the flavor and taste of the grains are also improved. Compared with common commercially available meal replacement powder, the product realizes directional domestication fermentation of probiotics in a composite coarse cereal environment, so that the product has high viable organism stability, ensures the colonization ability of the probiotics in cereal powder, realizes synergistic improvement of nutrition and flavor, contains certain functional components, and has relatively good sugar controlling and stabilizing ability.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

An objective of the present invention is to provide a technique for preserving cells or tissues in an unfrozen state while maintaining good viability. Cells or tissues are preserved in an unfrozen state in a liquid composition containing deacylated gellan gum or a salt thereof and an acidic polysaccharide or a salt thereof that maintains a random coil state in a divalent metal cation medium and can be crosslinked via divalent metal ions. The acidic polysaccharide may be alginic acid. The liquid composition may further contain a metal cation such as calcium ion.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LM2 and application of the lactobacillus plantarum strain LM2. The strain has relatively high GABA (gamma-aminobutyric acid) production capacity and nitrite degradation capacity, has a bacteriostatic effect on intestinal pathogens such as staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and campylobacter jejuni, has a good bacteriostatic function, also has good acid resistance and cholate resistance, and can be used for preparing a bacterial strain for treating intestinal diseases. The strain can well survive in artificial intestinal juice and artificial gastric juice; in order to increase the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LM2 is obtained by researching the accelerant, and the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 99% or above, the yield of GABA is increased to 3.0 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

The invention relates to a gastric acid-resistant sustained-release intestinal bacteria capsule based on bionic mucous membrane-enzymolysis response and a preparation method thereof. The gastric acid-resistant sustained-release intestinal bacteria capsule comprises a core layer, an adhesive layer, an outer-layer composite film and a protective coating from inside to outside, the core layer consists of intestinal flora and a multifunctional freeze-drying protective agent; the adhesive layer is composed of trehalose, N-acetyl glucosamine and MUC2 recombinant protein; the outer-layer composite film is composed of a base material and a functional filler; the protective coating is composed of beewax and chitosan. The anti-gastric acid sustained-release intestinal bacteria capsule based on bionic mucosa-enzymolysis response provided by the invention has excellent gastric acid resistance, and the gastric acid resistance is remarkably improved; good slow release performance is achieved, and accurate slow release of enzymatic hydrolysis response can be achieved; the colonization efficiency of the intestinal bacteria can be effectively improved; the storage stability is good. The preparation method provided by the invention has the advantages of simplified steps, short time consumption, controllable core operation time and obviously shortened whole process, and is beneficial to keeping the activity of the flora.

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The invention provides a bovine colostrum probiotic compound preparation as well as a preparation method and application thereof. The compound preparation provided by the invention comprises the bovine colostrum powder subjected to activity protection and the compound probiotics, and the bovine colostrum powder subjected to activity protection is obtained by adopting a microcapsule double-layer embedding and freeze-drying technology, so that the gastric acid retention rate (2h) of immune globulin in the bovine colostrum powder is greater than or equal to 90%, and IgG contained in the bovine colostrum powder is ensured to be efficiently delivered to intestinal tracts to play an immune regulation role. Wherein the compound probiotics comprise four stages of functional echeles formed by more than or equal to 6 strains, and the four stages of functional echeles comprise colon colonization major flora, gastric acid-resistant pioneer flora, antibacterial peptide secretion auxiliary flora and inflammation regulation backup flora. Through cooperation of the components in the compound preparation, the compound preparation has excellent immune enhancement and metabolic regulation functions.

The invention belongs to the technical field of microorganisms, and discloses a bifidobacterium animalis subsp. Lactis XB02 strain capable of inhibiting pathogenic bacteria and improving inflammation, the preservation number of the bifidobacterium animalis subsp. Lactis XB02 strain is CGMCC (China General Microbiological Culture Collection Center) No.34942, and the preservation date is June 18, 2025. Meanwhile, the probiotic composition adopting the bacterial strain XB02 also comprises a lactobacillus rhamnosus XB01 bacterial strain. The XB02 strain disclosed by the invention has excellent bacteriostatic activity, especially for vibrio parahaemolyticus and gardneria; the atopic dermatitis symptom can be obviously improved; the intestinal mucosa permeability is reduced, and the intestinal barrier function is enhanced; and when the strain is combined with the XB01 strain for use, a synergistic sleep promoting effect is shown. The invention further provides a probiotic composition containing the strain, a preparation and application of the probiotic composition and the preparation in food, medicine or health care products.

The invention discloses a pseudomonas capable of efficiently and rapidly degrading chloramphenicol and application of the pseudomonas. The name of the strain is Pseudomonas sp. MH-W1, the preservation number of the strain is GDMCC No: 66907, and the strain is preserved in the Guangdong Microbial Culture Collection Center on September 1, 2025. The strain can tolerate 200 mg / L of chloramphenicol; 100 mg / L of chloramphenicol can be degraded within 8 h, and the degradation rate reaches about 80%. According to the method provided by the invention, the degradation time can be remarkably shortened, the degradation efficiency is improved, the degradation condition is mild, the operation is simple and convenient, large-scale application is easy to realize, and the method has a wide industrial popularization prospect. The strain is an aerobic bacterium, is fast in growth and reproduction, simple to prepare, low in cost, convenient to use and high in efficiency, and can be used for repairing the environment polluted by chloramphenicol.

The invention belongs to the technical field of biology, and particularly relates to a complex microbial inoculant for promoting formation of a biological membrane, a microcapsule and application of the complex microbial inoculant. The complex microbial inoculant comprises lactobacillus mucus fermentation FMBL L24016DMX with the preservation number of CCTCC NO: M20241583 and bifidobacterium longum subsp. Longum FMBL B241770LS with the preservation number of CCTCC NO: M 20241993, the complex microbial inoculant can grow on a culture medium containing prebiotics, the biofilm formation amount is the highest when the complex microbial inoculant is co-cultured in a culture medium containing isomaltooligosacharide, the complex microbial inoculant is prepared into a single-layer microcapsule or a double-layer microcapsule, and the complex microbial inoculant can be used for preparing the probiotics. The endophytic fungus has good embedding rate and viable count, can resist gastrointestinal environment, has good heat resistance, and has wide application prospects.

The invention relates to the technical field of agricultural fertilizers. More specifically, the invention relates to an extraction method of tenebrio molitor antibacterial peptide and application of the tenebrio molitor antibacterial peptide in preparation of tea tree anthracnose resistant fertilizer. According to the preparation method, the colletotrichum camelliae liquid is taken as an induction factor, the ultrasonic induction technology is utilized to strengthen the absorption of tenebrio molitor to the induction factor, meanwhile, a supercritical / ethanol cosolvent extraction process and a bionic affinity chromatography purification process are combined, the efficient extraction of the antibacterial peptide is realized, and the obtained antibacterial peptide has a relatively good inhibition effect on the colletotrichum camelliae. A tea tree anthracnose resisting leaf fertilizer is further prepared through the tenebrio molitor antibacterial peptide, the leaf fertilizer has a good prevention and treatment effect on tea tree anthracnose caused by camellia colletotrichum spinosus, meanwhile, tea growth can be promoted, and the yield reduction caused by anthracnose and nutrition deficiency of tea tree crops can be effectively reduced.

The invention relates to the technical field of microbial preparations, and particularly discloses bacillus velezensis HY16 fermentation liquor freeze-dried powder and a preparation method and application thereof.The preparation method includes the steps that hydroxyl of beta-cyclodextrin reacts with anhydride of maleic anhydride, double bonds are introduced to beta-cyclodextrin molecules, and then under the action of persulfate serving as an initiator, the beta-cyclodextrin and maleic anhydride react with each other to prepare beta-cyclodextrin HY16 fermentation liquor freeze-dried powder; and carrying out free radical copolymerization reaction on the beta-cyclodextrin and 2-acrylamide-2-methylpropanesulfonic acid to obtain the modified beta-cyclodextrin. Sulfonic acid groups in 2-acrylamide-2-methylpropanesulfonic acid are strong hydrophilic groups, the water solubility of beta-cyclodextrin can be remarkably improved, growth of ice crystals during freezing is delayed, mechanical damage of the ice crystals to strains is reduced, and meanwhile negative charges of the sulfonic acid groups can be effectively absorbed through electrostatic repulsion. The freeze-drying process is simple, aggregation and inactivation of fermentation active substances or strains in the freeze-drying process are prevented, the survival rate of viable bacteria in the freeze-drying powder is increased, the stability of active ingredients in fermentation liquor is kept, and then the algae-lysing effect is improved.