This invention discloses a method for preparing Yuanjiang
asparagus extract and its application. The method uses fresh Yuanjiang
asparagus tender stems as
raw material. After selection, washing, freeze-
drying, pulverizing, and sieving,
choline chloride and 1,2-
propanediol eutectic
solvent are added for constant-temperature extraction. After ultrasonic
temperature control,
cellulase and
pectinase are added for complex
enzymatic hydrolysis. After heating to inactivate the enzymes,
centrifugation is performed, and the supernatant is collected to obtain the crude extract of Yuanjiang
asparagus saponins. This method has high extraction efficiency, and the obtained asparagus saponins have strong
antioxidant activity, exhibiting strong
scavenging ability against
DPPH,
ABTS, and hydroxyl radicals, as well as Fe... 2+ The
chelation rate was high, and it showed significant
in vitro inhibitory effects on the proliferation of human
colorectal cancer cells Caco-2 and human
hepatocellular carcinoma cells HepG2. The
raw material used in this method is fresh, tender stems of Yuanjiang asparagus, which is widely available and of excellent quality. The extraction process is green,
environmentally friendly, and simple, not only improving the comprehensive utilization value of Yuanjiang asparagus but also providing a new method for extracting natural
saponin active ingredients. The obtained asparagus saponins can be used to develop
antioxidant and anti-tumor functional foods, showing
potential market prospects.