The invention discloses a sesame oil extraction method. The method comprises the following steps that 1, sesame seeds are cleaned, husked, smashed, subjected to water regulation and then subjected to freezing treatment; 2, microwave defrosting is conducted on the sesame powder subjected to freezing treatment; 3, the defrosted sesame powder is subjected to step-by-step enzymolysis, centrifugal separation is conducted after enzymolysis is completed, and sesame oil, emulsion, hydrolysate and residues are obtained; 4, the emulsion is subjected to ultrasonic treatment, ethyl alcohol is added, centrifugation is conducted, and the sesame oil is obtained. According to the sesame oil extraction method, the high-quality green sesame oil can be obtained, needed equipment is simple, operation is safe, the obtained sesame oil is free of residual solvent, and the final total oil extraction rate can reach 97.91%.