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Piping structure for extremely low temperature liquid and ship provided with the same
ActiveCN116685524Bincrease supply
A piping structure for extremely low-temperature liquid is applied to a ship that stores an extremely low-temperature liquid having a boiling point of -196°C or lower under normal pressure. The piping structure includes a low-temperature pipe (10) that is arranged along a structural member (4) of the ship (1) at a position that is apart upward from the structural member (4) and that allows the extremely low-temperature liquid to flow therethrough, and a storage region dividing member (20) that is provided on the structural member (4) and that divides a storage region (AR) capable of storing an evaporation promoting liquid on the structural member (4), the evaporation promoting liquid receiving a drop of liquefied air formed on a surface of the low-temperature pipe (10) and evaporating the drop.
Owner:KAWASAKI JUKOGYO KK
Multifunctional integrated rice drying equipment
ActiveCN224121649UImprove uniformityHelp to break upDrying gas arrangementsDrying chambers/containersAgricultural engineeringElectric machinery
The utility model provides multifunctional integrated rice drying equipment, and relates to the technical field of rice drying, the multifunctional integrated rice drying equipment comprises a bottom plate, and a conveying mechanism is arranged above the bottom plate; the conveying mechanism comprises a drying box, the bottom face of the drying box is fixedly connected with the upper surface of the bottom plate, two inclined plates are arranged in the drying box, two side plates are fixedly connected to the upper surface of each inclined plate, seven protruding columns are arranged on the upper surface of each inclined plate, and flow guide blocks are fixedly connected to the side faces, close to each other, of each set of side plates. According to the multifunctional integrated rice drying equipment, structures such as the inclined plates, the side plates, the convex columns and the flow guide blocks are arranged, so that rice can be promoted to continuously roll and move in the drying process, the position and state of the rice in the drying box are changed, and the problem that the bottom of the rice in existing equipment is difficult to fully contact with heat due to contact with conveying equipment is effectively solved; and the rice drying and heating uniformity is greatly improved, and the vibration motor can enable the inclined plate to vibrate.
Owner:TAIZHOU YUEXIANG RICE IND CO LTD
A rhizosphere iron mobilizing bacterial strain for alleviating iron deficiency chlorosis of litsea cubeba and application thereof
This invention discloses a rhizosphere iron-promoting bacterial strain that alleviates iron deficiency-induced premature aging in Litsea cubeba and its applications. It belongs to the field of agricultural microbiology and plant mineral nutrition regulation technology. This invention provides related products and specific applications. The strain of this invention possesses both ferric iron (Fe3+) reduction ability and siderophore production ability. On the one hand, it can promote the conversion of ferric iron to ferrous iron (Fe2+); on the other hand, it can chelate and activate iron in the rhizosphere environment through siderophores, thereby increasing the supply of active iron in the rhizosphere of Litsea cubeba. After the Litsea cubeba rhizosphere iron-reducing bacterium LcB6 is reintroduced, it can increase the chlorophyll content of Litsea cubeba leaves and the soluble iron content of the plant, increase the content of ferrous iron and available iron in the rhizosphere soil, and improve iron deficiency-induced premature aging phenotypes such as leaf yellowing and decreased plant growth vigor. This provides a new bacterial resource and application pathway for the regulation of rhizosphere iron nutrition in Litsea cubeba.
Owner:HUNAN ACAD OF FORESTRY
Gentamicin C1a high-yield biological manufacturing method based on metabolic network modeling and reverse metabolic engineering modification
PendingCN121950971Aimprove consistencyHigh metabolic network coverageBacteriaMicrobiological testing/measurementMetabolic network modellingMetabolic network
The invention belongs to the field of microbial engineering, and provides a gentamicin C1a high-yield biological manufacturing method based on metabolic network modeling and reverse metabolic engineering renovation, which comprises the following steps: S1, constructing a genome scale metabolic model of a target production strain, predicting and screening a first target gene in positive correlation with gentamicin biosynthesis by using the model; s2, performing whole genome sequencing on the gentamicin high-yield mutant strain, and identifying a second target gene related to the high-yield character through comparative genomics analysis; s3, combining the first target gene predicted in the step S1 and the second target gene identified in the step S2, and constructing to obtain a recombinant engineering strain; s4, carrying out fermentation culture on the recombinant engineering strain, and supplementing materials in the fermentation process; by remarkably improving the production efficiency and strain performance of gentamicin C1a, important technical support and theoretical basis are provided for green biological manufacturing of antibiotics.
Owner:EAST CHINA UNIV OF SCI & TECH
Agricultural economic water-saving device
ActiveCN224178826Uquality improvementGuaranteed filtering effectGeneral water supply conservationWatering devicesWater sourceSurface water
The utility model belongs to the technical field of agriculture, and provides an agricultural economic water-saving device which comprises an installation plate, supporting grooves oppositely installed at the upper end of the installation plate, a water tank arranged between the two supporting grooves, a water outlet pipe communicated with one side of the water tank, a water pump arranged at the upper end of the installation plate, an input end of the water pump communicated with the water outlet pipe, and an output end of the water pump communicated with a pipeline. Nozzles are arranged on the outer surface of the pipeline. Rainwater is effectively collected through the water tank in rainy days, reasonable utilization of water resources is achieved, irrigation water source supply is increased, and dependence of agricultural production on underground water or surface water is reduced, so that water cost is saved, water resource environment protection is facilitated, sustainable development of agricultural production is achieved, and the ecological agriculture development requirement is met. The filter screen can effectively intercept sundries such as fallen leaves, branches and soil, it is ensured that rainwater entering the water tank is relatively clean, and the rainwater collecting quality is improved.
Owner:曲阜市尼山镇农业综合服务中心
Intelligent automobile charging system and method based on interaction of automobile machine and cloud end, and storage medium
PendingCN121871437Areduce anxietyincrease supplyCharging stationsOperating modesNew energyControl engineering
The invention discloses an intelligent automobile charging system and method based on interaction of an automobile machine and a cloud end and a storage medium, and relates to the technical field of new energy automobiles and charging control. The system comprises a vehicle machine end, a cloud end and a vehicle charging station end, the in-vehicle terminal comprises a man-vehicle interaction module, a vehicle-mounted positioning module, a margin detection module and an in-vehicle communication module; the cloud comprises a communication center, a site management module, an electric pile distribution module and a cloud server. The method is matched with the system, and the storage medium is matched with the method. According to the invention, based on the charging requirement content, the position information of the vehicle, the residual electric quantity of the vehicle and the expected available time, the most suitable charging pile is matched in the arranged vehicle charging station end and provided for the user, technical services meeting the use requirements can be provided for the user to the maximum extent, the anxiety of the user in charging the electric vehicle is reduced, and the user experience is improved. And the supply of the charging pile is optimized, and contributions are made to popularization and development of the electric automobile.
Owner:ZHONGTENG DAPANG INTERNET TECH (GUANGZHOU) CO LTD
Gene detection system for promoting andrographolide synthesis based on biosensor
PendingCN121975971AOptimize synthetic routeGuaranteed uptimeMicrobiological testing/measurementBiostatisticsGene ModificationGenetic engineering
The invention relates to the technical field of gene engineering detection, and particularly discloses a gene detection system for promoting andrographolide synthesis based on a biosensor, a key gene ApDof29 for regulating and controlling andrographolide synthesis is determined through whole genome analysis, gene expression profile research and co-expression network analysis, the core regulation and control effect of ApDof29 is verified, and the gene detection system for promoting andrographolide synthesis based on the biosensor is used for promoting andrographolide synthesis. The invention also develops a real-time dynamic monitoring system, and realizes non-invasive monitoring of andrographolide content accumulation, biosynthesis rate and metabolic intermediate concentration change by utilizing the fusion of a specific response element and a reporter gene. Through comprehensive calculation processing of an accumulation abnormal coefficient, a synthesis rate abnormal coefficient and a concentration change abnormal coefficient, the regulation and control effect of ApDof29 is dynamically evaluated, and a scientific basis is provided for subsequent gene modification.
Owner:江西省 中国科学院庐山植物园
A soil carbon-increasing and fertilizing method based on carbon-nitrogen ratio phased regulation of microbial community and application thereof in rice planting
PendingCN122581075AOvercome technical bottlenecks that are difficult to balanceAchieve high level enrichment
The application discloses a kind of based on carbon-nitrogen ratio phased regulation and control of soil carbon-increasing fertilization method of flora and its application in rice planting.The method carries out pre-rotting to rice straw and pig manure under the condition of phased carbon-nitrogen ratio, controls carbon-nitrogen ratio to be (25-28):1 in the first stage to promote carbon-fixing flora proliferation, adjusts carbon-nitrogen ratio to be (30-32):1 in the second stage to promote nitrifying bacteria group activation, and the obtained pre-rotted material is applied as base fertilizer into paddy field, and is based on the abundance monitoring result of soil carbon-fixing flora and nitrifying bacteria group during the rice growth period to carry out topdressing regulation and control.The application realizes the synergistic enrichment of paddy field carbon-fixing flora and nitrifying bacteria group by phased carbon-nitrogen ratio regulation and control, significantly improves soil organic carbon content while effectively reducing greenhouse gas emissions, improves rice yield and nitrogen utilization rate, and establishes a feedback control mechanism based on flora monitoring, to ensure the sustained and stable fertilization effect.
Owner:SICHUAN AGRI UNIV
Liquid container and image forming device
PendingCN121799058Aeasy to useEnsure smooth supplyElectrographic process apparatusPrintingInterior spaceImage formation
The invention provides a liquid container and an image forming device.The liquid container is installed in the image forming device.The liquid container comprises a first liquid storage part and a plurality of partition plates, the first liquid storage part is provided with a plurality of containing cavities used for storing liquid of different colors, and the partition plates are used for partitioning the inner space of the first liquid storage part to form the containing cavities; a plurality of spraying heads are further included, the multiple containing cavities communicate with the multiple spraying heads correspondingly, and liquid in the containing cavities flows out of the spraying heads; the multiple containing cavities comprise the first containing cavity used for containing black liquid, and the first containing cavity can meet more black printing requirements. The multiple ejection heads comprise the first ejection head communicating with the first containing cavity in the width direction of the liquid container, and the first ejection head is closer to the first containing cavity than other ejection heads, so that the path of black liquid flowing to the first ejection head is shorter, black liquid supply is faster, and smooth supply of black ink with the maximum requirement is guaranteed.
Owner:ZHUHAI PANTUM ELECTRONICS CO LTD
Full-hydraulic full-circle needle beam trolley and method for compressed air energy storage
ActiveCN121952634AEnsure construction safetyGuaranteed continuityUnderground chambersMeasurement devicesStructural engineeringControl theory
The invention discloses a full-hydraulic full-circle needle beam trolley and method for compressed air energy storage, and relates to the technical field of beam trolleys, the full-hydraulic full-circle needle beam trolley further comprises a moving frame, the moving frame is fixedly connected to the interior of a frame assembly and arranged on the outer side of a needle beam assembly in a sleeving mode, a sliding rail is fixedly connected to the outer side of the needle beam assembly, side grooves are formed in the two sides of the moving frame, and the sliding rail is fixedly connected to the sliding rail; a containing groove is formed in the bottom of the movable frame, a plurality of sliding blocks used for sliding are arranged between the movable frame and the sliding rail, and mounting pieces are arranged between the sliding blocks and the movable frame. A displacement sensor in the detection mechanism can accurately measure the actual abrasion depth of the bottom of the sliding block, and therefore distributed and normalized monitoring of the abrasion state of the sliding block is achieved. When it is monitored that the abrasion loss of any sliding block reaches the preset highest threshold value, the system can give out early warning in time to remind an operator to replace a specific sliding block, risks such as equipment clamping stagnation, precision reduction or sudden failure possibly caused by excessive abrasion of the sliding block are avoided, and construction safety and continuity are guaranteed.
Owner:CCCC SECOND PUBLIC BUREAU FOURTH ENG CO LTD
A catalytic system for highly selective synthesis of ethanol by hydrogenation of CO, CO2 or CO / CO2 mixed gas
The application discloses a catalytic system for high-selectivity synthesis of ethanol by hydrogenation of CO, CO2 or CO / CO2 mixed gas, and belongs to the field of CO and CO2 hydrogenation applications. The catalytic system comprises a heterogeneous catalyst and a homogeneous auxiliary agent. The catalytic system comprises a single-atom catalyst, an auxiliary agent and a solvent. The active component of the single-atom catalyst comprises at least one of Rh, Pd, Ir, Fe, Co, Ni and Cu. The auxiliary agent comprises LiI. The solvent comprises at least one of water and methanol. The heterogeneous and homogeneous dual-path synergistic catalytic system constructed by the application shows excellent reaction activity, ethanol selectivity and cycle stability. The application shows the potential to realize more excellent CO, CO2 or CO / CO2 mixed gas hydrogenation reaction performance by precisely designing and coupling the heterogeneous and homogeneous catalysis.
Owner:JIANGNAN UNIV
Sorting line cigarette stacking detection device
ActiveCN224211322Uincrease supplyImprove work efficiencyConveyorsPackaging cigaretteStructural engineeringConveyor belt
The cigarette stacking detection device for the sorting line comprises a sorting conveyor belt, baffles are arranged on the two sides of the sorting conveying belt. A plurality of mounting racks are arranged on one side of the sorting conveyor belt; a scanning camera extends from the top of the mounting frame to the upper side of the sorting conveyor belt; two sliding grooves are oppositely formed in baffles on the two sides of the sorting conveying belt. Connecting rods are slidably arranged in two sliding grooves oppositely formed in the baffles on the two sides. The two connecting rods are fixedly provided with a guide plate on the upper side of the sorting conveyor belt; the two opposite connecting rods extend out of the baffles and are connected with supporting plates. A motor is arranged on one side of the sorting conveyor belt; a rotating shaft of the motor extends to be provided with a threaded rod and is rotationally connected with the baffle; the threaded rod penetrates through the supporting plate and is in threaded connection with the supporting plate; the baffle plate is provided with a height indication marking line opposite to the scanning camera. Cigarettes passing through the sorting conveyor belt are detected and scanned through the scanning camera so as to detect the stacking height of the cigarettes.
Owner:HEBEI TOBACCO CO XINGTAI CO
A stirred metal hydride hydrogen storage device and method of operation thereof
ActiveCN116557757BImprove hydrogen absorption/desorption performanceImprove responseContainer filling methodsFluid transferredMetallic hydrogenEngineering
This invention discloses a stirred metal hydride hydrogen storage device and its operation method. The device includes a cylindrical reaction vessel and an outer shell. The reaction vessel is disposed inside the outer shell, and a closed heat exchange fluid cavity is formed between the reaction vessel and the outer shell. A stirrer is provided on the inner axis of the reaction vessel. The stirrer consists of a rotating shaft and blades. The rotating shaft has a hollow structure inside, which forms a flow channel for the heat exchange fluid. The bottom end of the rotating shaft is connected to the cavity. The interior of the reaction vessel is filled with metal hydride. The hydrogen storage device provided by this invention realizes the circulation and mixing of metal hydride in the reaction vessel through the stirrer, continuously transporting the high-temperature, low-reaction-rate metal hydride to the vicinity of the heat exchange surface, effectively improving the rate of hydrogen absorption and desorption reactions, and improving the hydrogen absorption / desorption performance of the hydrogen storage tank.
Owner:SHANDONG UNIV
Water-cooled screw liquid full type water chiller
This invention relates to the field of refrigeration equipment technology, specifically to a water-cooled screw-type flooded chiller, comprising a chiller body and an evaporator. The evaporator is mounted on the chiller body, with an inlet pipe at its bottom and an outlet pipe at its top connected to a screw compressor on the chiller body. This invention uses a float plate and guide assembly to vertically raise and lower the tube bundle according to the refrigerant level: when the system load decreases and the liquid level drops, the tube bundle moves downwards synchronously, always remaining submerged in liquid refrigerant, avoiding the loss of heat exchange capacity due to the upper part of the traditional fixed tube bundle being exposed to gaseous refrigerant; when the load increases and the liquid level rises, the tube bundle moves upwards synchronously to a shallow liquid level area near the liquid surface, where the static pressure is lower, effectively overcoming the defect of traditional fixed tube bundles at the bottom where excessive static pressure inhibits refrigerant boiling. This ensures that the tube bundle is in the optimal heat exchange position under different loads, thereby significantly improving the overall heat exchange efficiency and the chiller's comprehensive energy efficiency ratio.
Owner:ANHUI NORLAND TECH CO LTD
A genetically engineered strain of Saccharomyces cerevisiae and its application
PendingCN122081104Aachieve synthesisPromote accumulationFungiMicroorganism based processesGenetic enhancementMicrobiology
This invention discloses a genetically engineered strain of *Saccharomyces cerevisiae* and its application in cholesterol synthesis. The strain was obtained through multiple genetic engineering steps, starting with *Saccharomyces cerevisiae* SquP1: DHCR24 gene was integrated at the YPL062W site to achieve 7-dehydrocholesterol synthesis; the ERG6 promoter was replaced with the ERG7 promoter and the DWF5 gene was integrated to achieve cholesterol synthesis; the TGL3, TGL4, and ROX1 genes were knocked out to enhance cholesterol accumulation; the FLX1 and ZWF1 genes were integrated to strengthen cofactor supply; the HXK1 promoter was replaced and the ERG11 gene was integrated to optimize the synthesis pathway; and auxotrophic genes were reintroduced to obtain the CC37N strain. This strain achieved a maximum yield of 6.23 g / L in a 5L bioreactor fermentation, providing an excellent strain for efficient cholesterol biosynthesis.
Owner:EAST CHINA UNIV OF SCI & TECH
A heat-mass synergistic high-efficiency transmission reinforced heat transfer device
The application discloses a kind of reinforced heat transfer devices of heat mass synergic high-efficiency transmission, belong to phase change heat transfer technical field;The device includes condensing cover plate, the bottom of the condensing cover plate is sealed to connect evaporating cover plate, the inner side of the condensing cover plate is equipped with hydrophilic / hydrophobic combined surface, the condensing cover plate and evaporating cover plate are sequentially equipped with sidewall wick, auxiliary reflux zone wick from top to bottom between the condensing cover plate and evaporating cover plate, the auxiliary reflux zone wick is equipped with capillary reflux column and is inserted, the center of the auxiliary reflux zone wick is equipped with central boiling zone wick.The device makes hydrophilic pattern gather liquid and then returns auxiliary reflux zone wick through capillary reflux column, and then quickly returns central boiling zone wick under the action of gradient capillary force, through the synergic design of condensing end hydrophilic / hydrophobic combined surface and reflux path, the structure of wick is optimized, the working medium is quickly refluxed and bubble is efficiently overflowed, and the heat transfer performance and operating stability of the device are improved.
Owner:TIANJIN UNIV
Scroll compressor
PendingCN122589699ASuppress friction lossInhibit wear
Disclosed is a scroll compressor. In the scroll compressor, an oil supply groove can be formed between a plurality of first key grooves of a main frame, and the oil supply groove can be formed between an outer circumferential surface of a scroll disk support portion and an imaginary circle passing through the radial center of the first key groove. By this, oil flowing along the periphery of the cross slip ring is trapped in the oil supply groove, rapidly moves to the first key groove, and thus friction loss and / or wear between the first key groove and the first key inserted in the first key groove can be effectively suppressed. This can also be effective at initial startup and / or intermittent operation of a low-pressure upper compression type scroll compressor using a pressure difference oil supply method.
Owner:LG ELECTRONICS INC
Updraft high-efficiency fierce fire staggered combustion system
ActiveCN224316169URealize the core function of forced air supplyImprove ejection abilityDomestic stoves or rangesGaseous fuel burnerCombustion systemMarine engineering
The utility model discloses a high efficiency's fierce fire staggered floor combustion system of upper air inlet, include: furnace head subassembly, furnace base, blast box, air blower and fierce fire air outlet structure, blast box and air blower set up in the below of range panel, the blast outlet of fierce fire air outlet structure is located the above of range panel, towards the injection port of injection channel, blast box is equipped with the blast connecting port of connecting fierce fire air outlet structure, and air blower can send air through blast box to the blast outlet of fierce fire air outlet structure, the utility model discloses, break through upper air inlet space limit, realize efficient blast, the core innovation is in the ingenious adoption " blast source lower -positioned, air -supply path up -going, air -out point upper -positioned " split type design. Through special fierce fire air outlet structure, air is accurately delivered to the injection channel injection port near the above of range panel, realizes the core function of forced air supply. This design perfectly adapts the structural characteristics of all upper -positioned of upper air inlet burner injection system.
Owner:ZHONGSHAN HONGFU HARDWARE CO LTD
Hub module for motor vehicle, motor vehicle
PendingCN121947426AIncrease braking powerguaranteed loadBraking element arrangementsAxially engaging brakesElectric driveControl theory
The invention relates to a wheel hub module (33) for a motor vehicle, having an electric drive device (1) and having a wheeled brake device (8), the wheel hub module (33) having a housing (34) with at least one first housing part (2) for the drive device (1), the wheeled brake device (8) having a brake disc (9) and an actuating device (35), the actuating device (35) being arranged in the housing part (2), the brake disc (9) being arranged in the housing part (2), and the actuating device (35) being arranged in the housing part (2). The actuating device (35) has at least one first brake piston (12), and wherein the first brake piston (12) is movably guided in the first brake cylinder (15) in the direction of the brake disc (9) and is operatively connected to a first brake pad (10) associated with the brake disc (9). According to the invention, the first brake cylinder (15) is integrally formed with the first housing part (2).
Owner:ROBERT BOSCH GMBH
Device for sealing at least one hole in a body
Owner:SIKA TECH AG
Video playing method and device in map, equipment and computer storage medium
ActiveCN115203345BImprove interactive richnessincrease supplyVisual data miningVideo data browsing/visualisationComputer graphics (images)Target control
The application provides a video playing method and device in a map, equipment and computer readable storage medium; the method can be applied in the field including but not limited to map, navigation, automatic driving, intelligent traffic and the like, and the method comprises the following steps: in response to the interactive operation of the target control in the map display interface, a first acquisition request is sent, the first acquisition request is used to acquire target video data, the target video data corresponds to the target control; the response information of the first acquisition request is received, and the target video data is acquired based on the response information; a video playing window is displayed in the map display interface, and the video playing window is used to play the target video data. Through the application, the information quantity and interactive richness provided by the electronic map can be improved.
Owner:TENCENT TECHNOLOGY (SHENZHEN) CO LTD
An engineered bacterium for producing gallic acid and application thereof in producing gallic acid
ActiveCN121271770BIncrease productionhigh yieldBacteriaMicroorganism based processesGallic acid esterBiomanufacturing
The present application belongs to the field of metabolic engineering and biological manufacturing technology, and particularly relates to an engineered bacterium for producing gallic acid and application of the engineered bacterium in producing gallic acid. The present application realizes dynamic balance between growth of the engineered bacterium and synthesis of gallic acid product by systemically regulating key metabolic nodes in the production process of gallic acid, such as improving activity of hydroxylase, regulating flow direction of pyruvic acid and improving supply of aromatic amino acid precursors, so as to significantly improve yield and carbon yield of gallic acid. It is found through experiments that the yield of gallic acid of the engineered bacterium prepared by the present application reaches 12.5 g / L in a shake flask experiment and 109.8 g / L in a 3 L fermenter; and the carbon yield is improved to 0.64 g / g glucose, which is the highest level reported in the literature at present.
Owner:BEIJING UNIV OF CHEM TECH
Archaea phosphatidylinositol production strain as well as construction method and application thereof
PendingCN121991819Aincrease supplyImprove thermal stabilityFungiMicroorganism based processesPhosphatidylinositolEngineering
The invention provides an archaea phosphatidylinositol producing strain as well as a construction method and application thereof. The molecular formula of the archaea phosphatidylinositol is C49H83O11P, and the archaea phosphatidylinositol is Saccharomyces cerevisiae which is used for over-expression of G1PDH, GGGPS, DGGGPS, CarS, AIPS and INO1. The archaea phosphatidylinositol is a saccharomyces cerevisiae which is used for over-expression of G1PDH, GGGPS, DGGGPS, CarS, AIPS and INO1. The strain has relatively high thermal stability, and the obtained archaea phosphatidylinositol uDoPhPI is also proved to be capable of forming lipidosome and has very high thermal stability.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
Self-splitting squalene production strain and application thereof in preparation of squalene
PendingCN121759328Apromote rapid accumulationincrease supplyFungiMicroorganism based processesIsopentenyl pyrophosphateIsomerase
The invention provides a squalene production strain capable of automatically cracking and application of the squalene production strain in preparation of squalene. The strain is obtained by taking saccharomyces cerevisiae as an original strain and enhancing the activity of a gene encoding the following enzyme, the enzyme comprises acetyl coenzyme A transferase, 3-methyl-3-hydroxyglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, mevalonate kinase, mevalonic acid-5-phosphate kinase, mevalonic acid-5-pyrophosphate decarboxylase, isopentenyl pyrophosphate isomerase, farnesyl pyrophosphate synthase and squalene synthase. The invention further discloses a preparation method of the enzyme. After the engineering bacterium finishes efficient synthesis of squalene, cells are cracked to efficiently release squalene accumulated in the cells into fermentation liquor, so that separation and purification of downstream products are more convenient, downstream processes are simplified, the cost is reduced, and the production economy is integrally improved.
Owner:KETAYA BIOTECHNOLOGY (JIANGSU) CO LTD
Genetically engineered Max Kluyveromyces yeast and its applications
ActiveCN121801726BSynthetic efficient and stableincrease supplyFungiHydrolasesHeterologousBatch fermentation
This invention discloses a genetically engineered strain of *Kluyveromyces martensii* and its applications, belonging to the field of bioengineering technology. The invention discloses a genetically engineered *Kluyveromyces martensii* strain, firstly obtained through screening a strain that produces high levels of ergosterol; based on this, it induces and isolates... Eye A haploid strain was constructed and then metabolically optimized through the introduction of heterologous DHCR24 and DWF5 genes, weakening of the competitive pathway gene ERG6, increasing the metabolic flux of the post-squalene pathway during sterol synthesis, peroxisome compartmentalization engineering of the mevalonate pathway, knockout of the hypoxia-inhibiting transcription factor ROX1, improved cofactor supply, and optimization of the yeast lipid pathway, resulting in a high-cholesterol-producing engineered strain. Through fed-batch fermentation, efficient and stable cholesterol synthesis was achieved, with a yield of 7.64 g / L, laying the foundation for cholesterol biomanufacturing.
Owner:ENZYMECODE BIOTECHNOLOGY CO LTD
Apparatus and method for manufacturing a tubular tire member
Owner:THE YOKOHAMA RUBBER CO LTD
A tunnel kiln cooling device
This utility model relates to the field of tunnel kiln technology and discloses a tunnel kiln cooling device, including a tunnel kiln cooling body, a cooling assembly on the tunnel kiln cooling body, and an air outlet assembly on the cooling assembly. This tunnel kiln cooling device, by setting an air guide column inside the air outlet nozzle, with multiple evenly distributed air inlets and spiral-shaped guide grooves on the air guide column, allows the airflow blown by the quenching fan to be evenly dispersed through the air inlets after entering the air outlet nozzle. Guided by the spiral guide grooves, the airflow forms a stable rotating flow state, which is beneficial for optimizing the airflow direction. Simultaneously, the multiple evenly distributed air outlet vertical pipes and air outlet nozzles allow cooling air to be blown out simultaneously from multiple directions and positions, forming a uniform cooling airflow field. This allows the cooling airflow to better act on the surface of the object, improving the uniformity of cooling.
Owner:HUBEI DAQING TECH CO LTD
Video playing method in map, device, equipment and computer storage medium
ActiveHK40075274BImprove interactive richnessincrease supplyComputer hardwareComputer graphics (images)
The application provides a video playing method and device in a map, equipment and computer readable storage medium; the method can be applied in the field including but not limited to map, navigation, automatic driving, intelligent traffic and the like, and the method comprises the following steps: in response to the interactive operation of the target control in the map display interface, a first acquisition request is sent, the first acquisition request is used to acquire target video data, the target video data corresponds to the target control; the response information of the first acquisition request is received, and the target video data is acquired based on the response information; a video playing window is displayed in the map display interface, and the video playing window is used to play the target video data. Through the application, the information quantity and interactive richness provided by the electronic map can be improved.
Owner:TENCENT TECHNOLOGY (SHENZHEN) CO LTD
Pickling apparatus for steel
The utility model discloses a pickling device for steel, including base, the top of base is installed with pickling tank, the one side of pickling tank forms the bottom groove of downward extension, the bottom of bottom groove is installed with the oscillator, the one side of bottom groove is provided with the storage box for depositing pickling agent, the bottom of one side of storage box is provided with the liquid outlet pipe, the top of storage box is provided with the cover, and the top one side of storage box has the storage groove. The utility model provides a pickling device for steel, and pickling tank is supported through setting base, and the bottom groove of pickling tank one side extension cooperates oscillator, prevents the impurity after cleaning from adhering again on steel, and storage box is used for depositing pickling agent in bulk, and the liquid outlet pipe is convenient for pickling agent and is guided into pickling tank, realizes the convenient supply of pickling agent, and the cover can reduce the volatilization or pollution of pickling agent in storage box, and the storage groove can be used for placing the tool or small -size accessories required for pickling, and the operation convenience is improved.
Owner:ZHESHANG ZHONGTUO GRP (ZHEJIANG) NEW MATERIAL TECH CO LTD
An L-homoserine-producing strain, its construction method and application
ActiveCN121022708Befficient productiongood synthesis effectCarbon-nitrogen lyasesBacteriaHeterologousHomoserine synthesis
This invention provides an L-homoserine-producing strain, its construction method, and its applications. The strain does not contain plasmids. E.coli W3110, as a chassis strain, had the relA and thrB genes knocked out, and heterologously expressed genes derived from... Clostridium acetobutylicum The gapC gene and the aspB gene derived from Bacillus subtilis were overexpressed, along with ppc, asd, and thrA. fbr ,rhtA,pntAB,spoT fbr Genes; the strain described above can effectively improve the L-homoserine synthesis efficiency by knocking out genes of the L-homoserine degradation pathway, enhancing the expression of key enzymes in the pathway, optimizing the L-homoserine transport system, increasing the reducing power NADPH content, and enhancing the ability to withstand amino acid starvation. It is genetically stable, requires no addition of resistance substances, has high acid production efficiency, and has good L-homoserine synthesis capacity.
Owner:TIANJIN UNIV OF SCI & TECH