398 results about "Hydroxylation" patented technology

The invention relates to a three-dimensional boron nitride skeleton, a high-thermal-conductivity insulating composite material and a preparation method and application thereof, and belongs to the technical field of composites.The preparation method of the three-dimensional boron nitride skeleton comprises the steps that S1, hydroxylation treatment is conducted on boron nitride, and hydroxylated boron nitride is obtained; s2, carrying out surface modification on the hydroxylated boron nitride by adopting a silane coupling agent to obtain modified boron nitride; s3, uniformly mixing the modified boron nitride with saccharides, and performing compression molding to obtain a prefabricated blank; and S4, putting the prefabricated blank in water, and dissolving to remove saccharides, thereby obtaining the three-dimensional boron nitride skeleton. The preparation method of the three-dimensional boron nitride skeleton provided by the invention is simple in process, environment-friendly and low in cost, and the prepared three-dimensional boron nitride skeleton has relatively good structural strength and stability.

The invention discloses an ultrasonic activation type piezoelectric gel dressing and a preparation method and application thereof.The surface of a system is an antibacterial hydrogel layer, the antibacterial hydrogel layer is composed of polyethyleneimine (PEI) and polyvinyl alcohol (PVA) cross-linked hydrogel, and ultrasound and current can be transmitted to a deep area; bT-dNO piezoelectric nanoparticles are packaged inside, and BT-dNO is prepared by coating a nitric oxide (NO) donor on the surface of piezoelectric barium titanate (BT) subjected to hydroxylation and dopamine modification. Under the stimulation of low intensity focused ultrasound (LIFU), the donor can release NO as required, and the BT nanoparticles can generate reactive oxygen species (ROS) and output piezoelectric current. The dressing system has an effective combined repair effect, excellent biocompatibility and good anti-inflammatory ability.

The invention relates to the technical field of adhesives, and provides a high-thermal-conductivity modified silane polyether adhesive and a preparation method thereof.The silane-terminated polyether sealant is prepared from silane modified polyether resin, modified filler, a flame retardant, a silane coupling agent, a catalyst, an antioxidant and a water removal agent; the modified filler is obtained by grafting a hydroxylated AlN filler or a hydroxylated BN filler or a hydroxylated AlN and hydroxylated BN compound filler with silane under the catalysis of a weak acid to form a Si-O-Al or / and Si-O-B covalent bond network, and then mixing the Si-O-Al or / and Si-O-B covalent bond network with nano silicon dioxide particles of which the surfaces are coated with polydopamine. Through a multi-dimensional filler modification technology and reasonable matching of other auxiliaries, the problem of synergism of heat conduction, flame retardance and mechanical properties of the MS adhesive under a high filling system is solved, and the material is suitable for a new energy power battery module.

The application discloses an improved electrolytic synthesis method of 3,6-dichloropicolinic acid, which comprises the following steps: adopting a diaphragm electrolytic cell, taking an aqueous solution containing 3,4,5,6-tetrachloropicolinic acid and a certain flow of CO2 gas as a cathode liquid, taking an aqueous solution of alkali metal hydroxide as an anode liquid, taking silver as a cathode, and taking a nickel-based material as an anode to carry out an electrolytic reaction, and obtaining 3,6-dichloropicolinic acid after the electrolytic reaction is completed. In the electrolytic process, the anode is separated from the cathode, so that corrosion is avoided; meanwhile, the CO2 gas is introduced to effectively control the occurrence of a hydroxylation side reaction, the molar yield of a hydroxyl byproduct is reduced from 20% to 35% to 2% to 8%, the molar yield of 3,6-dichloropicolinic acid is increased from 45% to 50% to above 80%, and the highest can reach 92.2%.

The invention discloses a preparation method of 2-chloro-4-(4-fluoro-2-methylphenyl)-5-aminopyridine as an elinnetin intermediate, and belongs to the technical field of medicine synthesis. According to the invention, cheap and easily available 3-nitro-4-hydroxypyridine is used as an initial raw material, and the 2-chloro-4-(4-fluoro-2-methylphenyl)-5-aminopyridine is synthesized at low cost and high yield through the steps of halogenation, coupling, hydroxylation, reduction, condensation and the like. The raw materials used in the whole process are simple and easy to obtain, the reaction conditions are milder, and the reaction route is short. According to the invention, the Suzuki coupling reaction is firstly carried out, and then the hydroxylation of the pyridine 2 position is carried out, so that the use of an anhydrous reagent and harsh anhydrous reaction conditions are effectively avoided, and the reaction cost is remarkably reduced. In addition, the method is easy and convenient to operate, good in stability, high in total yield and suitable for industrial large-scale production.

The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

The invention discloses a composite material modified electrode based on a functionalized single-walled carbon nanotube and application thereof, and belongs to the technical field of electrochemistry, the modified electrode comprises a glassy carbon electrode and a functionalized single-walled carbon nanotube-glucose composite material coating coated on the surface of the glassy carbon electrode; the mass ratio of functionalized single-walled carbon nanotubes to glucose in the coating is (0.2-5): 1, and the functionalized single-walled carbon nanotubes comprise at least one of hydroxylated single-walled carbon nanotubes, carboxylated single-walled carbon nanotubes and aminated single-walled carbon nanotubes, preferably hydroxylated single-walled carbon nanotubes. The composite material modified electrode provided by the invention has a large number of active sites, and by effectively utilizing the synergistic effect of the hydroxylated single-walled carbon nanotubes and glucose, the modified electrode shows excellent selective recognition capability on amino acid enantiomers and also shows good stability; and the method has a good application prospect in quantitative analysis of the amino acid enantiomers.

The invention discloses a method and a device for degrading bisphenol A by using a propane matrix membrane bio-membrane reactor, and belongs to the technical field of microorganism application. The method specifically comprises the following steps: inoculating activated sludge into a membrane bio-membrane reactor with a basic inorganic salt culture medium, and introducing propane to promote the growth of a bio-membrane on hollow fiber membrane filaments in the membrane bio-membrane reactor, so as to obtain the membrane bio-membrane reactor after membrane formation is completed; the method comprises the following steps: introducing bisphenol A-containing wastewater to be treated into a membrane bio-membrane reactor, continuously introducing propane, and inducing microorganisms to express alkane monooxygenase and cytochrome P450 monooxygenase, thereby promoting hydroxylation of bisphenol A and degrading bisphenol A into a downstream product of which the toxicity is lower than that of bisphenol A. The propane is used as a carbon source and an electron donor, the propane can be efficiently utilized to provide a safe and good environment for biodegradation of bisphenol A, and secondary pollution possibly caused by a traditional carbon source is avoided.

The invention provides a preparation method and application of a modified PVC adsorbent for gold extraction of carbonaceous gold ore, and belongs to the technical field of organic polymer materials and resource utilization. Based on construction of a hydroxylation-xanthate esterification reaction and bifunctional adsorption sites, a series of chemical modification paths of acetate nucleophilic substitution-hydrolysis-carbon disulfide alkali treatment are established, and xanthate functional groups with high reaction activity are accurately constructed on an inert PVC framework. Sulfur atoms in xanthate groups of the modified PVC are excellent electron donors and can generate strong chemical adsorption with metal impurities or polar sites on the surface of carbon, meanwhile, Van der Waals interaction between a main chain of the modified PVC and the carbon is reserved, and unmodified PVC or single hydroxylated PVC does not have the synergism of chemical adsorption and physical adsorption, so that the modified PVC can be used for preparing the carbon-modified PVC composite material, and the carbon-modified PVC composite material can be used for preparing the carbon-modified PVC composite material. And leap-type improvement of the adsorption capacity and selectivity of organic carbonaceous in minerals is realized.

The utility model provides an energy-saving device for separating and purifying materials after hydroxylation reaction of phenol and hydrogen peroxide, and relates to the technical field of chemical synthesis. Materials obtained after phenol hydrogen peroxide hydroxylation reaction are separated and purified through the extraction tower, the extraction agent recovery tower, the first phenol removal tower, the tar removal tower, the second phenol removal tower, the catechol removal tower, the light component removal tower and the hydroquinone tower, the whole technological process is simple, the yield is high, and the high-purity hydroquinone product and the high-purity catechol product can be obtained; according to the utility model, the extraction tower is adopted to replace a dehydration rectifying tower in the traditional process to remove phenol-containing wastewater, so that the phenol recovery rate is improved, and the subsequent wastewater treatment burden is reduced; and the tar removal tower is arranged behind the phenol removal tower I, so that the retention time of tar impurities in the whole process system is shortened, and the process safety is improved. According to the utility model, the high-temperature waste heat at the top of the dephenolization tower I is recovered by combining waste heat recovery with a mechanical steam recompression energy-saving technology, so that the process energy consumption of the operation of the whole device is effectively reduced.

A near-atomic-scale electrolytic machining method based on an interface self-assembly biomask comprises the steps that firstly, hydroxylation pretreatment is conducted on the surface of an anode of a workpiece, then the surface of the anode is covered with an organic solvent phase containing phospholipid monomers, water-phase electrolyte drops are introduced, molecular self-assembly driving force at an oil-water-metal three-phase interface is utilized, and the surface of the anode of the workpiece is subjected to near-atomic-scale electrochemical machining. And rapidly forming a layer of ultrathin, continuous and stable support phospholipid bilayer mask on the surface of the workpiece in situ. Then, biological nanochannels are embedded in the biomimetic mask to construct a confinement ion transport path. And finally, a precise pulse electric field is applied, electrolyte ions are driven to be strictly limited in the nanometer channel and transmitted to the surface of the workpiece, local anodic dissolution is triggered, and therefore localized material removal of the nanometer scale or even the near-atomic scale is achieved. According to the method, the technical bottleneck that the high-quality phospholipid bilayer is difficult to prepare on the high-surface-energy metal surface is solved, and a new way is provided for ultra-precision electrolytic machining.

The application discloses a kind of generation (4S) 4-hydroxyisophorone monooxygenase mutant and preparation method thereof, the monooxygenase is cytochrome P450 BM3 Monooxygenase from bacillus megaterium. The cytochrome P450 BM3 Monooxygenase mutant has high stereoselectivity and high reaction activity to catalyze isophorone hydroxylation, mutant R47L / Y51F / F81W / A191T / N239H / I259V / A276T / A328I / A330S / L353I / I401L / L437LL catalyzes isophorone to obtain (4S) 4-hydroxyisophorone diastereomeric excess rate can reach 95%, yield can reach 96%; the cytochrome P450 BM3 Monooxygenase mutant catalyzes isophorone synthesis (4S) 4-hydroxyisophorone process is simple, environmental protection, and industrialization prospect is good.

The invention discloses 10-hydroxydecanoic acid as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, Candida lipolytica is taken as a fermentation thallus, the Candida lipolytica contains alkane monooxygenase (CYP450), alcohol dehydrogenase (ADH), fatty alcohol oxidase (FAO) and fatty aldehyde dehydrogenase (FALDH), amplification and activation are performed on the Candida lipolytica in a seed culture medium, and the Candida lipolytica is obtained. The method comprises the following steps: inoculating a seed solution into a liquid culture medium, enabling a strain to adapt to a culture environment in advance, reducing strain death caused by environmental mutation after inoculation, inoculating the seed solution into the liquid culture medium, culturing until OD600 is 45-55, realizing high-density enrichment of the strain, being beneficial to increasing the content of CYP450, ADH, FAO and FALDH, adding decane and Tween 80, and enabling the Tween 80 to be capable of increasing the permeability of a cell membrane and increasing the reaction rate, the CYP450 is used for catalyzing decane to carry out hydroxylation, and ADH, FAO and FALDH are used for carrying out oxidation, so that the high-purity 10-hydroxydecanoic acid is obtained.

The present invention relates to a cosmetic composition comprising 2,4-diaminopyrimidine-3-N-oxide and / or one of its organic or mineral acid salts and / or one of its hydrates, piroctone olamine, niacinamide, and at least 1% by weight of a particular hydroxylated compound. The invention also relates to a cosmetic treatment method for human keratinous materials such as skin, particularly scalp, hair, and / or eyelashes, comprising at least the application of the cosmetic composition according to the present invention. The present invention further relates to the use of said cosmetic composition to treat hair loss and / or promote the regrowth of keratin fibers.

The application belongs to the field of compound synthesis and particularly relates to a photocatalytic preparation method of quinoxaline-2,3-dione compounds, which comprises the following steps: preparing a raw material solution containing quinoxaline-2-ketone, mercaptan, a photocatalyst and acetonitrile, and performing a photocatalytic reaction under light to obtain a quinoxaline-2,3-dione compound product; the mercaptan is C1-C4 alkyl mercaptan, and the -SH is connected to a primary carbon or a secondary carbon. The application adopts a photocatalytic method to perform hydroxylation on the C3 position of quinoxaline-2-ketone, and further controls the combination of the mercaptan and acetonitrile solvent to improve the photocatalytic reaction effect of C3 hydroxylation.

The present application relates to a kind of cytochrome P450 oxidase CYP716C52 protein, and the CYP716C52 protein coding gene, the protein can catalyze hydroxylation of maytenic acid C2 position to generate triptolide acid C, in turn participate in triptolide biosynthesis.

We are disclosing derivatives of 5-hydroxylated polymethoxyflavones. The derivatives offer enhanced oral bioavailability and improved intestinal permeability. The derivatives of the invention release the biologically active 5-hydroxylated polymethoxyflavones that are activated by chemical or enzymatic processes in the GI Tract. The derivatives are constructed as bioreversible prodrugs containing the bioactive molecules covalently linked with lipids like fatty acids, glycerides or phospholipids. The derivatives are useful against inflammatory conditions and aging. Formulations including the derivatives are also disclosed.

The invention discloses a preparation method and application of high-activity recombinant collagen, and relates to the field of biological medicine, and the preparation method comprises the following steps: S1, providing engineering bacteria containing a recombinant collagen expression vector, and performing seed culture; s2, inoculating the seed solution into a fermentation culture medium for fermentation culture; s3, in the fermentation process, when the thallus density reaches a preset value, a signal factor without an animal source is added into a fermentation system, and the signal factor is bionic polypeptide; s4, after the signal factors are added, the fermentation conditions are adjusted, and target collagen expression is induced; according to the preparation method and the application of the high-activity recombinant collagen, an endogenous metabolic network of bacteria is actively regulated and controlled by accurately adding animal-source-free bionic polypeptide signal factors in the fermentation process of engineering bacteria, and correct folding and specific post-translational modification of target collagen are induced; therefore, the hydroxylation rate and the biological activity of the recombinant collagen are improved, and the structure and the function of the recombinant collagen are closer to those of natural collagen of a human body.

The application relates to the technical field of nanofiltration membrane processing, in particular to a modification and recycling method of waste nanofiltration membranes, which comprises the following steps: 1) cyclic normal-temperature cleaning of the nanofiltration membranes by using alkaline agents or acid agents in combination with ultrasonic waves, and the cleaned nanofiltration membranes are washed clean by using deionized water or distilled water; 2) oxidation modification treatment in an oxidation reaction device by using an ozone oxidation method; 3) oxidation modification by using an oxidation agent in a modification device; 4) oxidation modification by using an acid medium oxidizing agent in the modification device; 5) hydroxylation modification by using a hydroxylation agent in the modification device; 6) modification and stabilization treatment by using a buffer stabilizing agent, and washing clean, so as to complete the modification and recycling treatment of the waste nanofiltration membranes. According to the modification and recycling method, the waste nanofiltration membranes can be utilized, the modification process is relatively simple, the membrane surface is not damaged after modification, the nanofiltration membrane flux is significantly improved after modification, and the interception rate meets the design and use requirements.

The invention provides a hyoscyamine 6 beta hydroxylase mutant as well as a preparation method and application thereof. The hyoscyamine 6 beta hydroxylase mutant at least has the following two sites or amino acid mutation in functional equivalent sites compared with wild hyoscyamine 6 beta hydroxylase: the 14th site and the 97th site; the wild type hyoscyamine 6 beta hydroxylase has an amino acid sequence as shown in SEQ ID NO: 1. The obtained hyoscyamine 6 beta hydroxylase mutant has higher hydroxylation activity, and meanwhile, the epoxidation capacity disappears or is reduced. In industrial application, the catalytic efficiency can be remarkably improved, reagent waste is reduced, the production cost is saved, generation of redundant by-products in chemical reaction is reduced, and environmental pollution is reduced.

The invention belongs to the technical field of bioengineering, and particularly relates to a kluyveromyces marxianus engineering strain for recombinant expression of hydroxylated human III-type collagen and application of the kluyveromyces marxianus engineering strain. The Kluyveromyces marxianus engineering strain of the human type III collagen is obtained by carrying out recombinant expression construction on proline hydroxylase and a human type III collagen gene in the Kluyveromyces marxianus; according to the invention, two truncation designs and codon optimization are carried out on the existing natural human III type collagen amino acid sequence to obtain the human III type collagen C3S1 and C3S2, and the recombinant secretory expression in the kluyveromyces marxianus engineering strain is realized. Like hydroxylation modification of the human III type collagen, the human III type collagen C3S1 and the human III type collagen C3S2 which are subjected to recombinant expression have proline hydroxylation characteristics. The recombinant human III-type collagen is prepared by using the kluyveromyces marxianus engineering strain, the method is simple, the production efficiency is high, and large-scale production is easy.

The invention relates to the technical field of functional cotton fibers, in particular to a preparation method of deodorizing antibacterial far-infrared heating quick-dry cotton fibers, which comprises five steps of activation of cotton fibers and hydroxylation modification pretreatment of nano oxide, preparation of functional impregnation liquid, impregnation and liquid removal of the cotton fibers, cross-linking curing and post-treatment. The preparation method comprises the following steps: activating cotton fibers to fully activate active hydroxyl groups on the surfaces of the cotton fibers, hydroxyl groups of modified cyclodextrin and hydroxylated nano oxide, and carrying out nucleation addition reaction with isocyanate groups of water-based terminated polyisocyanate; the nucleation addition reaction is triggered by the consistency of the water-based terminated polyisocyanate deblocking temperature and the drying temperature and the relaxation setting temperature of the cotton fiber production process, so that the problems that the existing cotton fiber does not have the functions of deodorization, antibiosis and far infrared heating and is difficult to dry after being washed are solved.

This invention provides an aerogel-modified recycled polyol, its preparation method, and its applications, relating to the field of polyurethane. The preparation method of the aerogel-modified recycled polyol includes the following steps: S1: Under an inert atmosphere, a mixture comprising waste polyurethane particles, a diol, a nitrogen-based organosilicon compound, and hydroxylated aerogel is reacted to obtain a polyurethane depolymerization product; S2: The polyurethane depolymerization product is separated to obtain a first product and a second product, the first product including the aerogel-modified recycled polyol. The preparation method of the aerogel-modified recycled polyol provided by this invention introduces aerogel simultaneously for in-situ chemical grafting in a highly efficient alcoholysis reaction system, directly preparing the aerogel-modified recycled polyol. This method not only retains the advantages of mild reaction, low energy consumption, and high-quality recycled polyol, but also upgrades the technical goal from chemical recycling to the direct synthesis of high-performance materials.

The application discloses a kind of DHA algal oil capsule-containing modulated milk and its preparation method.The preparation method of modulated milk provided by the application comprises the following steps: 1) dissolving DHA algal oil and hydroxylated polycaprolactone at both ends in ethanol;2) quickly injecting the mixed solution obtained in step 1) into water to obtain DHA algal oil capsule mixture A;3) drying the mixture A to obtain DHA algal oil capsules;4) mixing the DHA algal oil capsules with water to obtain mixture B;5) mixing the mixture B with raw cow milk to obtain mixture C;6) homogenizing;7) UHT ultra-high temperature instantaneous sterilization;8) cooling to room temperature and then aseptically filling.The application solves the problem of instability of DHA algal oil in modulated milk by encapsulating DHA algal oil with hydroxylated polycaprolactone at both ends, so that DHA algal oil maintains good flavor in the product, is not oxidized, reduces the generation of fishy smell, and makes the product system more stable.

The invention relates to a process for the acylation of a solid material, known as the hydroxylated material, bearing hydroxyl groups (—OH), known as reaction-generating hydroxyls, which are accessible and capable of reacting with at least one fatty acid chloride in gaseous form, said hydroxylated material being scrolled through a chamber, known as the acylation chamber, delimiting an internal space under a gaseous atmosphere. The invention also relates to an acylation device for performing said process.

Fatty acids with one or more unsaturations and an odd-numbered hydrocarbon chain are described, wherein these fatty acids have the chemical structure of the therapeutically active metabolites of alpha-hydroxylated mono- or polyunsaturated fatty acids with an even-numbered chain. The compositions comprising these fatty acids, their medical uses, and their use as indicators of a patient's efficacy and / or response to treatment with the alpha-hydroxylated mono- or polyunsaturated fatty acids with an even-numbered chain from which they are derived are also described.

The invention relates to a TS-1 molecular sieve, a preparation method thereof and application of the TS-1 molecular sieve in phenol hydroxylation reaction, and according to the preparation method, tetraethyl orthosilicate, tetrapropylammonium hydroxide, tetrabutyl titanate and amino acid are used as raw materials, crystallization is performed firstly, and then calcination is performed to prepare the TS-1 molecular sieve. The TS-1 molecular sieve catalyst is rich in a large amount of framework titanium, has high-activity hexa-coordinated titanium species, and can be used for efficient catalysis of phenol hydroxylation reaction. Compared with the prior art, the catalyst has excellent catalytic performance and excellent para-selectivity, and is simple in process, low in cost, good in stability and easy to popularize.

This invention discloses a heme protein mutant and its application in the preparation of 5-hydroxy-L-tryptophan, belonging to the fields of enzyme engineering and biocatalysis. This invention uses heme protein A2 as the starting protein and provides a method for direct catalysis with the participation of hydrogen peroxide. L A mutant of heme protein with β-tryptophan hydroxylation. The mutant constructed in this invention can be used in a solution containing 3 mmol / L... L In a reaction system containing 5-hydroxy-L-tryptophan, 15 mmol / L hydrogen peroxide, and 20 μmol / L purification enzyme, a 5-hydroxy-L-tryptophan conversion rate of 24.95% was achieved, compared to the single-mutant A2. M1 The percentage of 5-hydroxy-L-tryptophan was increased by approximately 8.63 times from 2.89%; and the area ratio of 5-hydroxy-L-tryptophan in the main monohydroxylation product peak was increased from approximately 49.8% to 97.1%, while the non-target product peak was reduced.

The application discloses a method for improving the moisture and heat resistance reliability of a bump connection structure, which comprises the following steps: (1) performing hydroxylation treatment on the bump connection structure for semiconductor device connection; (2) performing treatment on the bump connection structure with an organic compound to form a self-assembled molecular film; (3) performing dehydration treatment on the bump connection structure; and (4) performing etching on the self-assembled molecular film by using a reactive ion etching technology. Finally, the self-assembled molecular film with regular orientation and close arrangement is formed on the surface of the bump connection structure, especially at the undercut structure. The preparation method is simple, the self-assembled molecular film formed has high stability and corrosion resistance, and the reliability and stability of the bump in a moisture and heat environment can be effectively improved.