677 results about "Hydroxylation" patented technology

The invention discloses a preparation method of antistatic modified chinlon filaments, and relates to the field of chinlon filaments. When the antistatic modified chinlon filament is prepared, (formylmethyl) diethyl phosphate and 1-aminopyrrole react to prepare pyrrole diethyl phosphate; reacting the hydroxylated multi-walled carbon nanotube with pyrrole diethyl phosphate to obtain a modified multi-walled carbon nanotube; the preparation method comprises the following steps: reacting 2, 2 '-bipyridine-5, 5'-dicarboxylic acid with 2-amino-5-nitrophenol, and then reducing to obtain a benzoxazole diamine monomer; then, adipic acid, 1, 5-diaminopentane and a benzoxazole diamine monomer are subjected to a reaction, and modified polyamide is prepared; uniformly mixing the modified polyamide, the modified multi-walled carbon nanotubes, pyrrole, ferric trichloride and copper chloride, and performing melt spinning to obtain the antistatic modified polyamide filament. The antistatic modified chinlon filament prepared by the invention has excellent antistatic, flame-retardant, anti-aging and mechanical properties.

The invention relates to the field of plastic bags, and discloses a biodegradable plastic bag and a preparation method thereof, the biodegradable plastic bag comprises polylactic acid, poly (butylene adipate-co-terephthalate), a functional additive, modified starch, a lubricant and an antioxidant; the functional additive is prepared by grafting hydroxylated modified castor oil and isocyanated montmorillonite loaded titanium dioxide; the hydroxylation modified castor oil is prepared by modifying castor oil with chloroethyl isocyanate, then reacting with N-allylimidazole, and then reacting with 6-mercaptohexyl-1-alcohol; the isocyanated montmorillonite loaded titanium dioxide is prepared by reacting montmorillonite loaded titanium dioxide obtained by a sol-gel method with isophorone diisocyanate; the modified starch is prepared by reacting octadecanoyl chloride and acryloyl chloride with starch, then carrying out graft copolymerization with ethyl acrylate and further introducing glycidyl methacrylate, so that the plastic bag is endowed with excellent mechanical properties, biodegradability and antibacterial property.

The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TEM3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

The invention relates to the technical field of degradable PE (polyethylene) packaging materials, and discloses a PE film, a preparation process and application in tissue packaging, and the PE film is prepared by the following steps: by taking 7-(diethylamino) coumarin-3-carboxylic acid, 2-naphthyl methanol and epoxy chloropropane as raw materials, carrying out esterification reaction and quaternization reaction to prepare an epoxidized biodegradable organic photosensitizer; based on an epoxy-hydroxyl ring-opening reaction mechanism, hydroxylated titanium dioxide nanoparticles and polyvinyl alcohol resin are respectively modified by using an epoxidized biodegradable organic photosensitizer, and compounding is performed based on a pi-pi accumulation effect between aromatic rings to prepare the ultraviolet light dependent photo-biodegradable component. According to the present invention, the ultraviolet light dependent light-biological degradation component, the compatible component and the composite auxiliary agent are added to the polyethylene matrix, and blow molding is performed to obtain the PE film, and the film product shows excellent light-biological dual degradation performance, has actual use value, and can be used in the tissue packaging field.

The invention relates to a recombinant pichia pastoris genetically engineered bacterium for expressing recombinant full-chain-length humanized collagen as well as a preparation method and application of the recombinant pichia pastoris genetically engineered bacterium, and belongs to the technical field of genetic engineering. The recombinant pichia pastoris genetically engineered bacterium disclosed by the invention expresses recombinant full-chain-length humanized collagen, and a ku70 gene of the recombinant pichia pastoris genetically engineered bacterium is knocked out and knocked into a recombinant 4-proline hydroxylase encoding gene. Recombinant full-chain-length humanized collagen is optimized, a 4-proline hydroxylase gene and a collagen gene are co-expressed in pichia pastoris to obtain hydroxylation modified recombinant collagen, and a corresponding recombinant pichia pastoris genetically engineered bacterium is provided, has proteolytic enzyme A defect and hydroxylation modification capability, and can be used for preparing a recombinant collagen product. The stability of the collagen in the fermentation process is improved. The recombinant full-chain-length humanized collagen provided by the invention is 100% homologous with III-type collagen derived from a human body, so that the immune risk is extremely low, and the biological activity is more perfect.

The invention belongs to the technical field of biological medicines, and particularly discloses a lignin janus hydrogel patch as well as a preparation method and application thereof. The lignin janus hydrogel patch comprises a substrate layer and a tissue adhesion layer, wherein the substrate layer is pre-stretched, one surface of the substrate layer is subjected to hydroxylation treatment, and the tissue adhesion layer is attached to the hydroxylated surface of the substrate layer through lignin-based hydrogel and is subjected to photo-initiated free radical polymerization; the invention also discloses an application of the lignin janus hydrogel patch in preparation of a patch for promoting soft tissue defect or wound healing. In the lignin janus hydrogel patch disclosed by the invention, the lignin janus hydrogel patch has different adhesion properties, and the adhesion surface has excellent adhesion property, mechanical property and antibacterial property; after contacting with wet tissue of a wound, a strain program can be started to perform mechanical self-constriction, thereby effectively helping the wound to be closed and accelerating the repairing speed.

The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

The invention discloses a photopolymer type holographic recording medium and a substrate processing method, and the method comprises the steps: providing a substrate which comprises a glass substrate or a resin substrate; providing a mixed solution of the photopolymer type holographic recording medium; performing hydroxylation treatment on the surface of the substrate to generate hydroxyl groups on the surface of the substrate; uniformly distributing the mixed solution of the photopolymer type holographic recording medium on the surface of a substrate, and baking, so that the compound with a plurality of isocyanate reactive functional groups and the polyisocyanate group compound in the photopolymer type holographic recording medium react to generate film-forming resin; the hydroxyl group on the surface of the substrate participates in the reaction, and the film-forming resin is connected to the substrate in a covalent bond manner. In this way, the adhesion between the photopolymer layer and the substrate can be improved without increasing the complexity of subsequent process operations.

The invention discloses a method for synthesizing phenolic compounds by catalyzing hydroxylation reaction of aromatic compounds by iron, which comprises the following step: in a solvent, catalyzing and oxidizing aromatic compounds to synthesize phenolic compounds by taking iron as a catalyst and amino acid or derivatives thereof as ligands under the action of an oxidizing agent and a reducing agent. According to the method, the phenolic compound is synthesized by carrying out hydroxylation on the C-H bond of the catalytic oxidation aromatic compound. The method has the advantages that the catalyst is wide in source and low in cost; the oxidant is wide in source, safe and environment-friendly; the compatibility of substrate functional groups is good, and the reaction selectivity is good; complex small molecules can be well converted into hydroxylated products, and the like. According to the method disclosed by the invention, under optimized reaction conditions, the yield of the separated target product can reach 95%.

The invention discloses preparation and application of a manganese hydroxylate modified zirconium-based metal organic framework, and belongs to the technical field of wastewater treatment. According to the preparation method, firstly, Mn oxide serves as a dopant, naphthalic acid serves as a frame connector, a zirconium-manganese bimetal organic frame is prepared through a hydrothermal method, and hydroxylation treatment is conducted through an alkali solution; mn replaces Zr atoms on a zirconium cluster to form high charge density with Zr, Mn and Zr can jointly participate in an electron transfer process and an active oxygen generation process to form double active centers, and the catalytic activity of a Fenton-like system is enhanced, so that the oxidative adsorption removal rate of As is remarkably improved. Experimental results show that the As removal rate can still reach 95.1% after five cycles, the targets of oxidative adsorption of As and reutilization are achieved, and the application of the zirconium-based metal organic framework in the field of catalytic adsorption of As is expanded.

The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

The present disclosure relates to a nanofiltration membrane preparation method, and a nanofiltration membrane prepared thereby. The preparation method comprises the following steps: preparing a casting solution, and curing the casting solution on a reinforcement material to form a base membrane; sequentially contacting the base membrane with a water phase solution and an oil phase solution so as to perform an interfacial polymerization reaction on the base membrane to form a polyamide functional layer, wherein the water phase solution includes a water phase monomer and an additive, the additive is a glycosylated sulfonamide compound, and the oil phase solution includes an oil phase monomer; and performing post-treatment to obtain a nanofiltration membrane, the post-treatment comprising hydroxylation treatment and crosslinking treatment. The nanofiltration membrane prepared by the preparation method of the present disclosure has high throughput and selective separation properties and excellent antibacterial properties, and can effectively prevent microbial pollution.

The invention discloses a method for preparing a micro-particle-size negative oxygen ion purifying agent from a nano material, and relates to the technical field of air purifying agents, the method comprises the following preparation steps: step 1, pouring 5-6.5 g of modified tourmaline powder and 10 g of an amino acid-nano particle compound into 200 mL of deionized water, then adding 0.5-0.54 g of polyvinylpyrrolidone, carrying out ultrasonic treatment for 30-40 min, then adding 0.5-0.54 g of polyvinylpyrrolidone into the deionized water, carrying out ultrasonic treatment for 30-40 min, and carrying out ultrasonic treatment for 30-40 min; carrying out ball milling until the particle size is 1-5 microns; 2, adding 2-2.6 g of a chitosan solution with the concentration of 1% after ball milling, adjusting the pH value of the system to 6, and stirring at 50 DEG C for 1 h; according to the method for preparing the micro-particle-size negative oxygen ion purifying agent from the nano material, tourmaline powder is modified, so that the surface hydroxylation degree of the tourmaline powder is improved, lattice defects are increased, the negative oxygen ion release efficiency is remarkably improved, meanwhile, the tourmaline powder is compounded with white carbon black, interface charge conduction can be enhanced, and continuous release of negative oxygen ions is further promoted.

The invention belongs to the technical field of antibiotic degradation, and particularly relates to a preparation method and application of a surface hydroxylation monatomic catalyst with a Co-O-Zn structure. Zinc acetate and cobalt acetate are used as metal sources, dimethylimidazole is used as a ligand, mixing reaction is carried out in a solvent, centrifugal washing, drying and calcining are carried out, the surface hydroxylation monatomic catalyst with a Co-O-Zn structure is prepared, the surface hydroxylation monatomic catalyst has an asymmetric Co-O-Zn configuration, a large number of hydroxyl groups exist on the surface, and the surface hydroxylation monatomic catalyst has the advantages that the surface hydroxylation monatomic catalyst has a good application prospect. The asymmetric coordination structure of Co-O-Zn can optimize the adsorption behavior of an oxidizing agent, effectively adjust the interaction of metal sites and the electronic structure in the catalysis process, significantly enhance the catalytic reaction, and have strong ability to resist the interference of external inorganic ions and organic matters.

The invention relates to the technical field of polyurethane compositions, in particular to a chlorotrifluoropropene-based high-temperature-resistant polyurethane composition and a preparation process thereof, and the chlorotrifluoropropene-based high-temperature-resistant polyurethane composition comprises the following raw materials: hydroxylated chlorotrifluoropropene, modified hexamethylene diisocyanate tripolymer, phthalic anhydride polyester polyol, nano silicon carbide whiskers and polyimide microspheres. A fluorine element is introduced into hydroxylated chlorotrifluoropropene, so that the thermal decomposition temperature of the material is remarkably increased, and thermal movement and oxidative degradation of a molecular chain at high temperature are inhibited; meanwhile, a rigid benzene ring structure of bisphenol A dihydroxyethyl ether improves the regularity of a molecular chain and reduces high-temperature softening deformation. The modified hexamethylene diisocyanate tripolymer forms a stable siloxane cross-linked network, so that the thermal stability of the material is further enhanced; in addition, an isocyanate group reacts with hydroxyl to generate a high-density carbamate crosslinking point, so that the regularity of a hard segment is enhanced, and the thermal stability of the material is improved.

The invention relates to a three-dimensional boron nitride skeleton, a high-thermal-conductivity insulating composite material and a preparation method and application thereof, and belongs to the technical field of composites.The preparation method of the three-dimensional boron nitride skeleton comprises the steps that S1, hydroxylation treatment is conducted on boron nitride, and hydroxylated boron nitride is obtained; s2, carrying out surface modification on the hydroxylated boron nitride by adopting a silane coupling agent to obtain modified boron nitride; s3, uniformly mixing the modified boron nitride with saccharides, and performing compression molding to obtain a prefabricated blank; and S4, putting the prefabricated blank in water, and dissolving to remove saccharides, thereby obtaining the three-dimensional boron nitride skeleton. The preparation method of the three-dimensional boron nitride skeleton provided by the invention is simple in process, environment-friendly and low in cost, and the prepared three-dimensional boron nitride skeleton has relatively good structural strength and stability.

The invention discloses an ultrasonic activation type piezoelectric gel dressing and a preparation method and application thereof.The surface of a system is an antibacterial hydrogel layer, the antibacterial hydrogel layer is composed of polyethyleneimine (PEI) and polyvinyl alcohol (PVA) cross-linked hydrogel, and ultrasound and current can be transmitted to a deep area; bT-dNO piezoelectric nanoparticles are packaged inside, and BT-dNO is prepared by coating a nitric oxide (NO) donor on the surface of piezoelectric barium titanate (BT) subjected to hydroxylation and dopamine modification. Under the stimulation of low intensity focused ultrasound (LIFU), the donor can release NO as required, and the BT nanoparticles can generate reactive oxygen species (ROS) and output piezoelectric current. The dressing system has an effective combined repair effect, excellent biocompatibility and good anti-inflammatory ability.

The invention belongs to the technical field of triple negative breast cancer, and particularly relates to application of a polypeptide capable of specifically blocking phosphorylation of metabolic enzyme PSAT1S337 and functional remodeling of PSAT1 in preparation of a triple negative breast cancer treatment product. The phosphorylated polypeptide targeting PSAT1S337 is designed, and it is found that the polypeptide obviously inhibits growth of triple negative breast cancer tumors and can improve the curative effect of anti-PD-1 immunotherapy at the animal level. Meanwhile, the invention further provides application of the reagent for detecting the phosphorylation level of the PSAT1S337 and / or the hydroxylation level of the GPX4P159 in preparation of products for diagnosing and prognosing the triple negative breast cancer. The invention discloses a molecular mechanism of functional remodeling of the serine metabolic enzyme PSAT1 in occurrence and development of cell ferroptosis and triple negative breast cancer, and provides a new thought and an effective scheme for research and development of anti-tumor drugs for targeted serine metabolism reprogramming and early diagnosis of tumors.

The invention relates to the technical field of adhesives, and provides a high-thermal-conductivity modified silane polyether adhesive and a preparation method thereof.The silane-terminated polyether sealant is prepared from silane modified polyether resin, modified filler, a flame retardant, a silane coupling agent, a catalyst, an antioxidant and a water removal agent; the modified filler is obtained by grafting a hydroxylated AlN filler or a hydroxylated BN filler or a hydroxylated AlN and hydroxylated BN compound filler with silane under the catalysis of a weak acid to form a Si-O-Al or / and Si-O-B covalent bond network, and then mixing the Si-O-Al or / and Si-O-B covalent bond network with nano silicon dioxide particles of which the surfaces are coated with polydopamine. Through a multi-dimensional filler modification technology and reasonable matching of other auxiliaries, the problem of synergism of heat conduction, flame retardance and mechanical properties of the MS adhesive under a high filling system is solved, and the material is suitable for a new energy power battery module.

The invention relates to a bacteriostatic washing machine tank cleaning block composition and a preparation method thereof, and the bacteriostatic washing machine tank cleaning block composition comprises the following components: peroxide salt, a surfactant, a chelating agent, an acid substance, modified silicon dioxide, an active oxygen activator, a release agent, an adhesive, a filler and water; wherein the modified silicon dioxide is obtained by firstly hydroxylating silicon dioxide, then carrying out esterification reaction with hexadecenoic acid and linolenic acid, and finally polymerizing with sodium acrylate. The modified silicon dioxide can generate mechanical friction with stubborn dirt to promote the dirt to fall off, a high-crosslinking-degree net-shaped organic chain segment formed on the surface of the modified silicon dioxide has amphipathicity, after the modified silicon dioxide is compounded with the surfactant and other components, a synergistic effect is generated to improve the decontamination effect, the compatibility of all the components can be improved, and the cleaning effect is improved. Falling stubborn dirt can be wrapped, and the decontamination effect is further improved; all the components are compounded, so that a good antibacterial effect and the like are achieved, and a good prospect is achieved.

In some aspects, the present disclosure pertains to systems for forming hydrogels that comprise (a) a radiopaque polyamino compound and (b) a reactive polymer comprising a plurality of hydrophilic polymer segments and a plurality of reactive moieties, wherein the reactive moieties are reactive with amino groups of the radiopaque polyamino compound, and wherein the radiopaque polyamino compound is produced by a method that comprises: (i) converting hydroxyl groups of a polyhydroxylated radiopaque compound to amino groups, (ii) converting vicinal diol groups (—CHOHCH2OH groups) of a polyhydroxylated radiopaque compound into hydroxyaminoethyl groups (—CHOHCH2NH2 groups), or (iii) converting vicinal diol groups of a polyhydroxylated radiopaque compound into aminomethyl groups (—CH2NH2 groups). In other aspects, the present disclosure pertains to hydrogels formed from such systems and to methods of treatment using such systems.

The invention relates to a phenol hydroxylation reaction liquid separation and purification method, which comprises: dehydrating a phenol hydroxylation reaction liquid, and carrying out decoking to reduce the operation temperature of subsequent separation so as to obtain a first material; carrying out vacuum rectification on the first material through a dephenolization tower to obtain a second material; wherein the dephenolization tower comprises a first dephenolization tower and a second dephenolization tower, the second dephenolization tower and the first dephenolization tower are thermally integrated, tower top steam of the second dephenolization tower is used as a reboiler heat source of the first dephenolization tower, and meanwhile, tower top steam of the second dephenolization tower is condensed; the tower top operation pressure of the second dephenolization tower is higher than that of the first dephenolization tower; and separating the second material to obtain catechol and hydroquinone. The technical problem that the benzenediol product yield and the separation energy consumption are difficult to balance in an existing phenol hydroxylation reaction liquid separation system is solved.

The invention provides a catalyst for synthesizing N-methylaniline and a preparation method thereof, the catalyst comprises an active component, a carrier and an auxiliary agent, the active component comprises oxygen-containing compounds of copper, zinc and chromium, the carrier is gamma-alumina subjected to surface hydroxylation treatment, and the auxiliary agent is a cerium-molybdenum bimetallic alloy. The preparation method comprises the following steps: loading the active component on the hydroxylation carrier through ultrasonic impregnation, drying, further loading the cerium-molybdenum auxiliary agent, and carrying out reduction alloying and roasting activation to form the composite catalyst. The catalyst is a regular cylinder, has the diameter of 4-6mm and the length of 4-6mm, has an optimized pore structure and surface properties, and is suitable for methylation reaction of aniline and methanol. According to the method, the dispersity of active components is improved through modified chromic oxide, the mass transfer efficiency is enhanced through a hydroxylation carrier, the synergistic catalysis effect of cerium-molybdenum alloy is combined, the reaction activity and selectivity are remarkably improved, meanwhile, side reactions are inhibited, the stability is enhanced, and the method can be widely applied to industrial production of N-methylaniline.

The invention relates to the technical field of rubber modification, in particular to a method for enhancing thermal stability and oxidation stability of ethylene propylene diene monomer rubber by using functionalized h-BN nanosheets. Comprising the following steps: S1, preparing a hydroxylated h-BN nanosheet; s2, synthesizing phosphazene modified boron nitride, namely purifying hexachlorocyclotriphosphazene for later use, then weighing 1g of h-BN-OH powder, grinding, pouring the powder into a three-necked flask containing 100ml of acetone, carrying out ultrasonic stirring for 2 hours, then adding 0.8 g of 4, 4-dihydroxybiphenyl, continuously stirring for 10 minutes, dropwise adding an acetone solution of HCCP and 5ml of triethylamine, adding tannic acid after reaction, cooling to room temperature after the reaction is finished, and filtering to obtain the phosphazene modified boron nitride. Washing and drying to obtain phosphazene modified boron nitride; and S3, preparing the phosphazene modified boron nitride / EPDM composite material. Through the synergistic effect of P / N groups and phenolic hydroxyl groups in the phosphazene modified boron nitride, the phosphazene modified boron nitride can achieve an excellent flame retardant effect under the condition of low flame retardant additive amount, and the use amount of the flame retardant is remarkably reduced.

The application discloses an improved electrolytic synthesis method of 3,6-dichloropicolinic acid, which comprises the following steps: adopting a diaphragm electrolytic cell, taking an aqueous solution containing 3,4,5,6-tetrachloropicolinic acid and a certain flow of CO2 gas as a cathode liquid, taking an aqueous solution of alkali metal hydroxide as an anode liquid, taking silver as a cathode, and taking a nickel-based material as an anode to carry out an electrolytic reaction, and obtaining 3,6-dichloropicolinic acid after the electrolytic reaction is completed. In the electrolytic process, the anode is separated from the cathode, so that corrosion is avoided; meanwhile, the CO2 gas is introduced to effectively control the occurrence of a hydroxylation side reaction, the molar yield of a hydroxyl byproduct is reduced from 20% to 35% to 2% to 8%, the molar yield of 3,6-dichloropicolinic acid is increased from 45% to 50% to above 80%, and the highest can reach 92.2%.

The invention discloses application of a reducing agent in heme peroxidase catalytic reaction, the reducing agent is selected from ascorbic acid, dehydroascorbic acid, gallic acid and pyrogallic acid, and the reducing agent has a remarkable promotion effect on oxidation reaction and hydroxylation reaction catalyzed by heme peroxidase. A new scheme is provided for improving the catalytic oxidation efficiency of the heme peroxidase, and the development and application potential is huge.

The invention discloses a preparation method of 2-chloro-4-(4-fluoro-2-methylphenyl)-5-aminopyridine as an elinnetin intermediate, and belongs to the technical field of medicine synthesis. According to the invention, cheap and easily available 3-nitro-4-hydroxypyridine is used as an initial raw material, and the 2-chloro-4-(4-fluoro-2-methylphenyl)-5-aminopyridine is synthesized at low cost and high yield through the steps of halogenation, coupling, hydroxylation, reduction, condensation and the like. The raw materials used in the whole process are simple and easy to obtain, the reaction conditions are milder, and the reaction route is short. According to the invention, the Suzuki coupling reaction is firstly carried out, and then the hydroxylation of the pyridine 2 position is carried out, so that the use of an anhydrous reagent and harsh anhydrous reaction conditions are effectively avoided, and the reaction cost is remarkably reduced. In addition, the method is easy and convenient to operate, good in stability, high in total yield and suitable for industrial large-scale production.

The invention relates to the technical field of virus detection, in particular to a method for enhancing animal virus detection sensitivity through optical fiber SPR probe surface modification, which comprises the following steps: S1, optical fiber SPR probe surface pretreatment: obtaining a hydroxylated surface; s2, gradient nanostructure modification: forming a gradient aperture surface topological structure; s3, carboxyl functionalization: immersing the substrate into a 3-aminopropyltriethoxysilane / methacrylic acid mixed solution, and constructing a carboxyl activation layer on the surface; s4, fixation of the aptamer: realizing directional fixation of the aptamer through EDC / NHS mediated carboxyl-amino coupling reaction; s5, non-specific site closing: carrying out cross-linking closing on the non-binding sites by adopting a thioacetamide / polydopamine composite solution; s6, virus detection: acquiring the surface refractive index variation through a phase modulation type SPR detection system; according to the invention, accurate fitting and quantitative correction of virus concentration are realized, and the stability and accuracy of detection are improved.

The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

The invention discloses a composite material modified electrode based on a functionalized single-walled carbon nanotube and application thereof, and belongs to the technical field of electrochemistry, the modified electrode comprises a glassy carbon electrode and a functionalized single-walled carbon nanotube-glucose composite material coating coated on the surface of the glassy carbon electrode; the mass ratio of functionalized single-walled carbon nanotubes to glucose in the coating is (0.2-5): 1, and the functionalized single-walled carbon nanotubes comprise at least one of hydroxylated single-walled carbon nanotubes, carboxylated single-walled carbon nanotubes and aminated single-walled carbon nanotubes, preferably hydroxylated single-walled carbon nanotubes. The composite material modified electrode provided by the invention has a large number of active sites, and by effectively utilizing the synergistic effect of the hydroxylated single-walled carbon nanotubes and glucose, the modified electrode shows excellent selective recognition capability on amino acid enantiomers and also shows good stability; and the method has a good application prospect in quantitative analysis of the amino acid enantiomers.