75 results about "Hydroxylation" patented technology

This invention provides an aerogel-modified recycled polyol, its preparation method, and its applications, relating to the field of polyurethane. The preparation method of the aerogel-modified recycled polyol includes the following steps: S1: Under an inert atmosphere, a mixture comprising waste polyurethane particles, a diol, a nitrogen-based organosilicon compound, and hydroxylated aerogel is reacted to obtain a polyurethane depolymerization product; S2: The polyurethane depolymerization product is separated to obtain a first product and a second product, the first product including the aerogel-modified recycled polyol. The preparation method of the aerogel-modified recycled polyol provided by this invention introduces aerogel simultaneously for in-situ chemical grafting in a highly efficient alcoholysis reaction system, directly preparing the aerogel-modified recycled polyol. This method not only retains the advantages of mild reaction, low energy consumption, and high-quality recycled polyol, but also upgrades the technical goal from chemical recycling to the direct synthesis of high-performance materials.

Fatty acids with one or more unsaturations and an odd-numbered hydrocarbon chain are described, wherein these fatty acids have the chemical structure of the therapeutically active metabolites of alpha-hydroxylated mono- or polyunsaturated fatty acids with an even-numbered chain. The compositions comprising these fatty acids, their medical uses, and their use as indicators of a patient's efficacy and / or response to treatment with the alpha-hydroxylated mono- or polyunsaturated fatty acids with an even-numbered chain from which they are derived are also described.

This invention discloses a heme protein mutant and its application in the preparation of 5-hydroxy-L-tryptophan, belonging to the fields of enzyme engineering and biocatalysis. This invention uses heme protein A2 as the starting protein and provides a method for direct catalysis with the participation of hydrogen peroxide. L A mutant of heme protein with β-tryptophan hydroxylation. The mutant constructed in this invention can be used in a solution containing 3 mmol / L... L In a reaction system containing 5-hydroxy-L-tryptophan, 15 mmol / L hydrogen peroxide, and 20 μmol / L purification enzyme, a 5-hydroxy-L-tryptophan conversion rate of 24.95% was achieved, compared to the single-mutant A2. M1 The percentage of 5-hydroxy-L-tryptophan was increased by approximately 8.63 times from 2.89%; and the area ratio of 5-hydroxy-L-tryptophan in the main monohydroxylation product peak was increased from approximately 49.8% to 97.1%, while the non-target product peak was reduced.

The application relates to the field of sewage treatment, and discloses a sulfur autotrophic denitrification biofilter composite filler and a preparation method thereof. The method comprises the following steps: carrying out a silane hydrolysis reaction on a silane coupling agent containing a mercaptan group to prepare a hydrolysis solution, and then adding hydroxylated polystyrene microspheres into the hydrolysis solution to carry out a grafting reaction and prepare the sulfur autotrophic denitrification biofilter composite filler. The method grafts mercaptan groups to the surface of the polystyrene microspheres by using the silane coupling agent containing the mercaptan group. The mercaptan groups can specifically combine with sulfur transport proteins on the cell membrane of sulfur autotrophic denitrification microorganisms, so that the sulfur autotrophic denitrification microorganisms can selectively colonize on the surface of the polystyrene microspheres, competition between other microorganisms and the sulfur autotrophic microorganisms can be avoided, the colonization stability of the sulfur autotrophic microorganisms is improved, and the biofilm formation efficiency is improved.

The present invention relates to a hair treatment process for keratin fibres, comprising: - a step of applying a bleaching composition, and then - a step (ii) of applying a cosmetic composition B1 comprising at least 0.5% by weight, relative to the total weight of the composition, of an amino-acid type compound and of a hydroxylated C2-C8 (poly)carboxylic acid, and then - a step (iii) of applying a cosmetic composition B2 comprising at least 0.5% by weight, relative to the total weight of the composition, of an amino-acid type compound and of a hydroxylated C2-C8 (poly)carboxylic acid, steps (ii) and (iii) being spaced apart by at most 2 hours. The present invention also relates to a hair treatment process for keratin fibres, comprising: - a step of applying a bleaching composition, and then a step of applying a cosmetic composition B1 comprising an amino-acid type compound, a hydroxylated C2-C8 (poly)carboxylic acid and at least 0.6% of a polysaccharide, and then a step of applying a cosmetic composition B2 comprising an amino-acid type compound, a hydroxylated C2-C8 (poly-)carboxylic acid and less than 0.6% of a polysaccharide.

The application belongs to the technical field of bio-chemical industry, and particularly relates to a P450 enzyme mutant and application of an engineering bacterium thereof in synthesis of calcifediol, the mutant is one or more point positions in an amino acid sequence based on a wild type P450 enzyme Vdh being mutated; the amino acid sequence of the wild type P450 enzyme Vdh corresponds to GenBank accession number CP069288.1. Through systematic molecular dynamics simulation, conservation analysis and substrate binding pocket engineering on the Vdh enzyme, multiple key mutation sites are successfully screened and obtained. The constructed combined mutant (such as I114R / N173M / Q310R, Vdh-M3) shows extremely high hydroxylation activity, and the pure enzyme activity can reach 9.8 times of the wild type enzyme, greatly improving the conversion rate from vitamin D3 to calcifediol.

This invention discloses a method for preparing a key intermediate of iodopyridine, using dimethyl malonate as a raw material, and sequentially undergoing dihydroxylation S1 → acetalization S2 → hydrolysis and acidification S3 → decarboxylation S4 to obtain iodopyridine. The raw materials of this invention are inexpensive and readily available in the market, and the reaction efficiency is high, improving the purity and yield of the product. Intermediates 1 and 2 obtained from the process do not require separation and purification operations and can be directly used in the next step of the reaction. The purification effect is significant, yielding intermediate products with a purity ≥99%, and the total yield of the five steps is as high as 65-70%, which is beneficial for large-scale production.

The application provides a microplastic treatment method in soil, and belongs to the technical field of microplastic treatment. The treatment method is as follows: raw biochar is prepared from agricultural straw, and then is modified by hydroxylation, magnetism and carboxylation; the obtained carboxylated biochar is double-coated by chitosan, acetic acid and sodium alginate; then the obtained double-modified biochar is activated, and a covalently immobilized composite targeting enzyme agent is fixed; the surface of the obtained covalently immobilized enzyme agent is loaded with a bacterial agent suspension by atomization spraying, and is adsorbed after standing; finally, the obtained microplastic treatment agent is applied to soil in the process of plant planting for microplastic treatment. The treatment method does not need to use harmful substances such as chemical oxidants, strong acids and strong bases, has no negative effect on soil animals and microbial communities, realizes source control, in-situ repair and long-term control of microplastic pollution, and provides stable ecological guarantee for sustainable planting of facility cucumbers.

This invention belongs to the field of novel compounds and relates to a novel method for preparing dichlorfenapyr metabolites. Dichlorfenapyr is subjected to a hydroxylation reaction, and then the resulting hydroxylated product is subjected to a chlorination reaction to obtain the dichlorfenapyr metabolites. The preparation method provided in this invention is simple and suitable for large-scale laboratory production. The product prepared by the method of this invention has high yield, high purity, and low cost.

Composition comprising a direct dye, a non-associative polysaccharide, a particular polycarboxylic acid, and a hydroxylated compound. The invention relates to a composition comprising: one or more direct dyes; one or more non-associative polysaccharides; one or more polycarboxylic acids of formula (I); one or more hydroxylated compounds selected from aromatic alcohols, C2-C6 aliphatic hydroxylated solvents, and mixtures thereof.

This invention relates to the field of bioremediation technology for environmental pollutants, specifically to a method for degrading side-chain-less polycyclic aromatic hydrocarbons (PAHs) using the dual-oxygenation function of polycopper oxidases. This invention is the first to discover and demonstrate that polycopper oxidases, without the need for exogenous mediators, possess the ability to catalyze the dual-oxygenation reaction of side-chain-less PAHs, directly adding two oxygen atoms from molecular oxygen to the aromatic ring of PAHs to generate the corresponding dihydroxylated intermediate. Under further catalysis by polycopper oxidase, this intermediate selectively undergoes ring-opening cleavage at specific carbon-carbon bonds in the aromatic ring via a free radical mechanism, ultimately generating low-toxicity, readily bioavailable monobenzene ring or straight-chain ester compounds. The method provided by this invention is mild, environmentally friendly, and achieves thorough degradation, offering a highly efficient and green new technology for the bioremediation of PAH-contaminated soil, water bodies, and industrial waste.

This invention discloses a method for hydroxylation modification of recombinant mussel foot protein and its application. The invention utilizes a whole-cell catalyst with tyrosinase catalytic activity to hydroxylate tyrosine residues in recombinant mussel foot protein. This method eliminates the need for cell disruption and protein purification steps. By immobilizing tyrosinase on the host cell surface through cell surface display, the recombinant mussel foot protein can directly contact the tyrosinase in the extracellular environment and undergo hydroxylation, thereby introducing dopamine groups into the protein to improve its underwater adhesion properties. This method is applicable to the hydroxylation modification of various types of recombinant mussel foot protein and has advantages such as high catalytic efficiency, good stability, simple operation, and low cost, making it valuable for industrial applications.

Provided is a method for preparing a recombinant human collagen. The method comprises: constructing a genetically engineered bacterium which expresses a prolyl hydroxylase and a recombinant human collagen; culturing the genetically engineered bacterium and performing digestion treatment by means of pepsin to obtain a recombinant human collagen type I or type III. The prolyl hydroxylase comprises P4Ha2, P4Hb and vP4H, and by means of controlling the ratio of the copy numbers of the genes thereof, the recombinant human collagen and a natural human collagen are consistent in hydroxylation modification. Also provided is a large-scale production method for the recombinant human collagen. The prepared recombinant human collagen has completely the same sequence composition and length and substantially the same hydroxyproline content as the natural human collagen, and also has a stable full-length triple helix structure and biological activity without immunogenicity and potential risk of viruses.

The present application relates to a kind of tetrahydroxy bile acid pharmaceutical composition and its preparation method and application.The present application provides a kind of pharmaceutical composition, it includes 100 weight parts of compound shown in formula I and 300~900 weight parts of saccharide.The present application also provides the use of the pharmaceutical composition in the preparation of drug for treating PFIC.The pharmaceutical composition of the present application can improve dose accuracy, solve the problem of poor taste of bile acid compound, easy to swallow, can improve the compliance of children drug.The pharmaceutical composition of the present application has good stability, easy to transport and store.In addition, the pharmaceutical composition of the present application realizes the stability of treatment effect under different doses, easy to accurately dose children according to body weight, while having good bioavailability, can realize good absorption and utilization in vivo.

The application provides a method for removing pollutants in water by utilizing a local thermal effect to promote a Fenton-like reaction, and uses a ruthenium-doped titanium dioxide nanobelt (TNB-Ru) as a catalyst; the TNB-Ru is prepared by using commercial anatase titanium dioxide nanoparticles as raw materials, first, a hydroxylated titanium dioxide nanobelt is prepared by using an alkaline hydrothermal method, then the titanium dioxide nanobelt is dispersed in a ruthenium chloride ethanol solution, and the ruthenium is loaded on the titanium dioxide nanobelt by using the anchoring effect of the surface hydroxyl group, and finally the target material is obtained. The TNB-Ru prepared by the method has excellent catalytic degradation performance, which is mainly due to the fact that the ruthenium dispersed on the titanium dioxide nanobelt generates a local plasmonic resonance effect to form a local high-temperature environment under visible light irradiation, and the Fenton-like reaction is accelerated.

The application discloses a preparation method of a gradient sulfur-doped silicon-based negative electrode material, the negative electrode material and application. The preparation method of the gradient sulfur-doped silicon-based negative electrode material comprises the following steps: metal reduction pretreatment of a silicon-based material by using a metal material to obtain a pretreated product; acid washing of the pretreated product, then water washing and drying to obtain an acid-washed product; treatment of the acid-washed product by using ammonia water, then water washing and drying to obtain a hydroxylated product; the hydroxylated product is placed in a furnace, heated under an inert atmosphere, gradient heating to at least two different temperatures and heat preservation, sulfur doping treatment of the hydroxylated product in the heat preservation stage, then further heating, carbon deposition, sieving and magnetic removal to obtain the gradient sulfur-doped silicon-based negative electrode material. The application can effectively improve the intrinsic conductivity and performance stability of the silicon-based negative electrode material, is beneficial to improving the specific capacity and initial efficiency of the material, and makes the material have higher rate and cycle performance.

This invention belongs to the field of plant genetic engineering and plant stress resistance biotechnology, and discloses... ZmCYP81Q32 Application of genes in improving nicosulfuron resistance in plants. This invention provides... ZmCYP81Q32 The nucleotide sequence of the gene, as shown in SEQ ID NO:1, encodes a cytochrome P450 enzyme that can directly bind to sulfonylurea herbicides and promote their hydroxylation metabolism. This reduces the accumulation of the parent herbicide in the plant, alleviates herbicide-induced growth inhibition, leaf chlorosis, and oxidative damage, and significantly improves plant tolerance to sulfonylurea herbicides, especially enhancing plant resistance to nicosulfuron. This invention can be widely used for crop herbicide resistance improvement, herbicide-tolerant germplasm creation, and related molecular breeding.

The present application belongs to the field of enzyme catalysis and bioengineering technology, and relates to a cytochrome P450 enzyme CYP153C3 and application thereof. The amino acid sequence of the enzyme is shown in SEQ ID NO. 1 of the sequence listing. The nucleotide sequence of the DNA molecule encoding the enzyme is shown in SEQ ID NO. 2 of the sequence listing. The cytochrome P450 enzyme CYP153C3 provided by the present application has catalytic activity on C8-C14 chain length saturated fatty acids, various unsaturated fatty acids and short-chain alkanes (octane, decane); meanwhile, the enzyme shows excellent ω-1 hydroxylation capacity on saturated fatty acids, unsaturated fatty acids and alkane substrates, has high regioselectivity, and can be used for green bio-manufacturing of high-value hydroxyl fatty acids and hydroxyl alkane compounds.

Methods of filling a feature on a semiconductor substrate may include performing a process to fill the feature on the semiconductor substrate by repeatedly performing first operations. First operations can include providing a silicon-containing precursor. First operations can include contacting the substrate with the silicon-containing precursor to form a silicon-containing material within the feature defined on the substrate. First operations can include purging the semiconductor processing chamber. First operations can include providing an oxygen-and-hydrogen-containing precursor. First operations can include contacting the substrate with the oxygen-and-hydrogen-containing precursor to form a silicon-and-oxygen-containing material within the feature defined on the substrate.

This invention discloses a magnetic imidazole-based composite material, wherein the composite material uses Fe3O4 as a magnetic core, and the surface is sequentially coated with a KH-570 coupling layer and a polyvinyl imidazole functional layer, and the surface is rich in imidazole functional groups; the preparation method of the composite material includes the following steps: (1) hydroxylation treatment of the magnetic core: Fe3O4 is dispersed in an alkaline solution for hydroxylation treatment to obtain Fe3O4 with a surface rich in hydroxyl groups; (2) KH-570 modification: The hydroxylated Fe3O4 obtained in step (1) is dispersed in an organic solvent and silane coupling is added. The reaction was carried out with KH-570 to obtain Fe3O4@KH-570; (3) 1-vinylimidazolium graft polymerization: Fe3O4@KH-570 obtained in step (2) was dispersed in an organic solvent, 1-vinylimidazolium monomer and initiator were added, and graft polymerization was carried out under an inert atmosphere to obtain Fe3O4@KH-570@VIM composite material; Application of the composite material prepared by the present invention in enriching and degrading PET microplastics; The preparation process of the present invention is simple and low in cost, and has good environmental and economic benefits.

The application discloses a recombinant Gibberella fujikuroi for blocking competitive metabolic branch to improve gibberellin yield and a construction method thereof, and belongs to the technical field of industrial microorganisms.The recombinant Gibberella fujikuroi provided by the application knocks out a p450-3 gene, the GA4 yield of the recombinant strain is increased to 718.6 mg / L from 585.9 mg / L of the original strain, is increased by 22.6%, and the growth characteristics of the strain are not significantly affected.Through blocking the 13-hydroxylation competitive branch, the application realizes the redirection of metabolic flow to non-13-hydroxylation product GA4 / 7, provides an efficient chassis strain and a construction method for directional production of gibberellin components, and has important industrial application value.

The present application belongs to the technical field of veterinary drug residue detection, and particularly relates to a detection method of neomycin and apramycin in veterinary drugs, wherein a hydroxylated silver nanowire dispersion liquid and a dopamine modified Ag NCs@TiO2 nanoparticle dispersion liquid are mixed in equal volume, then embedded in a PDMS flexible substrate material to obtain a flexible SERS substrate material, a spiked sample solution is dropped onto the surface of the flexible SERS substrate material, and then dried and subjected to SERS detection, a linear regression equation is fitted by taking the characteristic peak intensity of the antibiotic and the logarithmic value of the concentration of the spiked sample solution as variables, a sample solution to be detected is dropped onto the surface of the flexible SERS substrate material, and then subjected to SERS detection to calculate the residual concentration of the antibiotic in the sample solution to be detected. The present application adopts a surface enhanced Raman scattering technology (SERS) to detect the residual amounts of neomycin and apramycin in meat products, and the detection is faster and simpler.

The application relates to a method for evaluating adsorption of organic matters on silicon dioxide based on molecular dynamics simulation, which comprises the following steps: constructing a hydroxylated silicon dioxide surface model; wherein the density of silanol on the hydroxylated silicon dioxide surface is 0-9.4 OH / nm 2 ; constructing an organic matter molecular model and inserting the organic matter molecular model into the hydroxylated silicon dioxide surface model to obtain an organic matter / silicon dioxide model; performing molecular dynamics simulation on the organic matter / silicon dioxide model; and evaluating the obtained simulation result. The application directly reveals the interaction between different organic matter molecules and different hydroxylated silicon dioxide surfaces at an atomic scale, evaluates the interfacial activity of the formed silicon dioxide surfaces with different hydroxylization degrees and different ionization degrees, and can provide important theoretical guidance for improving the crude oil recovery rate, mineral flotation efficiency, solid surface self-cleaning and other fields.

The present invention relates to a cosmetic composition, preferably for hair care, comprising at least 1% by weight of amino acid compounds and at least 3% by weight of hydroxylated (poly)carboxylic acids, comprising 2 to 8 carbon atoms, and / or their salts. The invention also relates to a cosmetic treatment method, particularly for washing and / or conditioning hair, using said composition.

The present invention relates to a cosmetic treatment method for strengthening sensitized, weakened and / or damaged hair, comprising the application of a cosmetic composition comprising: - one or more amino acid type compounds, present in a total content of at least 0.5% by weight relative to the total weight of the composition, and - one or more hydroxylated (poly)carboxylic acids, comprising 2 to 8 carbon atoms, and / or their salts, present in a total content of at least 0.5% by weight relative to the total weight of the composition.

The application belongs to the technical field of photocatalytic materials, and particularly relates to a pyrene-based hydrogen bond organic framework composite photocatalyst as well as a preparation method and application thereof. The pyrene-based hydrogen bond organic framework is combined with hydroxylated nitrogen-doped titanium dioxide by electrostatic attraction, and a heterojunction composite material with selective adsorption-photocatalytic synergistic effect is constructed through structural modification, so as to solve the problems of weak adsorption capacity, low charge separation efficiency and poor degradation selectivity of traditional titanium dioxide. The generation and quenching balance of active oxygen are regulated through interface electron transfer, and the photocatalytic degradation efficiency of ciprofloxacin is significantly improved. The preparation process is mild and has good repeatability, the obtained material has excellent selective removal capacity and cyclic stability for ciprofloxacin and other antibiotics, can be used for efficient purification of refractory organic pollutants in water, and has good application prospect.

The present application relates to fine chemical technology field, especially to a kind of anti-micro pitting additive and its preparation and application.The present application uses starch masking method, uses hydroxylated boron nitride and O, O-dialkyl thiophosphoryl chloride to carry out chemical reaction to prepare this anti-micro pitting additive, and the core skeleton is hexagonal boron nitride nanosheet, one side contains hydroxyl functional group, the other side is covalently connected O, O-dialkyl thiophosphate, and the process condition used is mild;The anti-micro pitting additive prepared by the present application can be used as multifunctional lubricant additive, utilizes the filling and interlayer slip of boron nitride nanosheet, the chemical adsorption of hydroxyl surface and friction pair, the tribological chemical reaction of O, O-dialkyl thiophosphate and friction pair synergistic effect, obtains good extreme pressure, antiwear, anti-micro pitting and anticorrosion etc., suitable for long-acting stable friction and micro pitting lubrication protection under harsh working conditions.