66 results about "Tissue penetration" patented technology

The invention provides an activatable head and neck cancer diagnosis and treatment integrated nanoprobe and a preparation method and application thereof.The preparation method includes the steps that according to an original data matrix, an adaptive spectrum correction algorithm is applied to eliminate background noise of an instrument, and when the relative fluctuation of spectral intensity exceeds a preset threshold value by 5%, a sliding window filtering technology is adopted, the window size is 5 nanometers, and the spectrum intensity is obtained; generating a de-noised spectral data set; measuring the pH value of the head and neck cancer tissue microenvironment by using a pH value sensor, recording the emission peak position change of the near-infrared quantum dots when the pH value is less than 6.5 in combination with the de-noising spectral data set, analyzing the frequency band displacement by adopting a Gaussian fitting algorithm, and generating a mapping data set of the pH value and the frequency band displacement; and aiming at the activation spectrum fingerprint data set, applying a dynamic spectrum matching algorithm, comparing with the spectrum response characteristic data set, calculating a cosine similarity coefficient, and when the similarity exceeds 0.85, determining the activation state and the tissue penetration depth of the nanoprobe, and generating a diagnosis and treatment parameter adjustment instruction set.

The present invention provides a large Stokes shift near-infrared fluorescent probe for detecting HClO, its preparation method and biological imaging application, belonging to the field of bioanalysis technology. Based on the strategy of modifying the substituents at the 3,6 positions of xanthene, the present invention constructs a large Stokes shift near-infrared fluorescent probe for specifically recognizing HClO. Optical experiments show that the emission wavelength of the probe for detecting HClO is 776 nm, the Stokes shift reaches 148 nm, with strong selectivity, high sensitivity, and a detection limit as low as 4.95 nM, which can greatly reduce the interference of background fluorescence in biological imaging applications. The fluorescent probe of the present invention has almost no cytotoxicity and can be applied to the detection and imaging of HClO in living cells MCF-7 and rat liver tissues, with a tissue penetration depth of 140 μm.

The invention relates to a preparation method and application of narrow-band-gap W18O49-X nano brush Schottky junction antibacterial nanoparticles based on near-infrared light response. In order to solve the problems of low phototoxicity and low tissue penetrability caused by weak absorption of an existing W18O49-X near-infrared (NIR) region, the invention provides a preparation method and application of narrow-band-gap W18O49-X nano-brush Schottky junction antibacterial nanoparticles based on near-infrared light response, the nanoparticles have good antibacterial performance and biocompatibility, the preparation process is simple, the preparation cost is low, and the preparation method is suitable for industrial production. The W18O49-X coated Au Schottky junction is constructed by uniformly growing Au nanoparticles on the W18O49-X nano brush through in-situ reduction, so that the photothermal conversion efficiency is improved, and the generation of active oxygen is increased. The antibacterial rate of staphylococcus aureus and the antibacterial rate of escherichia coli reach 99% or above, the PTT and PDT synergistic antibacterial method can effectively avoid drug resistance of bacteria, and the mild photo-thermal effect can promote differentiation, proliferation and migration of cells and accelerate revascularization.

This invention discloses a lipid assembly loaded with 5-aminolevulinic acid and its preparation method. The lipid assembly is a hollow sphere with a core consisting of a hydrophilic phospholipid-chelating agent conjugate formed by chelation with a reactive phospholipid PEG reagent. The outer layer of the phospholipid-chelating agent conjugate is loaded with 5-aminolevulinic acid via hydrazone bonds, and then combined with a radionuclide as an internal light source excitation photosensitizer. The lipid assembly is loaded with 5-ALA via hydrazone bonds. Utilizing the acid-sensitive bond-breaking characteristics of hydrazone bonds and the prodrug properties of 5-ALA, combined with a radionuclide labeled on the surface of the lipid assembly as an internal light source excitation photosensitizer, this invention can overcome the limitations of external light penetration depth in traditional photodynamic therapy, achieving deep PDT treatment. Combined with chemotherapy drugs, it can minimize the toxic side effects on normal tissues during the combined use of radionuclides and photosensitizers in targeted therapy.

The invention belongs to the technical field of nano biological materials, and particularly relates to a near-infrared two-region excitation and emission lanthanide nano fluorescent probe as well as a preparation method and application thereof. Lanthanide ions with near-infrared two-region absorption characteristics are used as sensitizers, nanocrystalline with a co-doped structure formed by the lanthanide ions and activator ions is used as a core, and a pure-phase matrix material shell layer which is the same as a core lattice is grown on the surface of a core particle in situ. The nano fluorescent probe can generate tunable near-infrared two-region fluorescence emission with the wavelength of 1000 to 3000 nm under the excitation of near-infrared light with the wavelength of more than 1000 nm. The near-infrared two-region excitation and emission characteristics of the nano fluorescent probe can provide higher tissue penetration depth and signal-to-noise ratio for in-vivo imaging. The nano fluorescent probe shows excellent performance in living body angiography, dynamic observation of intestinal tracts and multi-channel near-infrared two-region imaging, and has wide application prospects in the fields of biomedical detection, early diagnosis of diseases, surgical navigation and the like.

A near-infrared fluorescent molecular probe with the "ESIPT + AIE" effect for detecting hydrogen sulfide has the following structural formula: #imgabs0# The aqueous solution of this fluorescent molecular probe is sensitive to the concentration of H2S, with a detection limit (LOD) of 0.83 μM (S / N = 3), showing high sensitivity; the maximum emission wavelength is 713 nm, having advantages such as strong tissue penetration, low background fluorescence interference, and little damage to cells / tissues, which is conducive to realizing the detection and / or imaging of endogenous H2S content; the linear detection range is 0 - 30 μmol / L, with a wider quantitative range and a broader application range.

The invention provides a near-infrared beta-galactosidase fluorescent probe as well as preparation and application thereof, and relates to the technical field of fluorescent probes, according to the near-infrared beta-galactosidase fluorescent probe, when beta-galactosidase exists, a D-galactosyl group can be split, meanwhile, self-elimination of p-hydroxybenzyl is removed, a fluorescent signal unit is released, and the beta-galactosidase can be detected by the near-infrared beta-galactosidase fluorescent probe. The fluorescence signal is obviously enhanced. Excitation and emission spectra of the near-infrared beta-galactosidase fluorescent probe are both in a near-infrared region, the fluorescence intensity at 760 nm is rapidly increased, the fluorescence intensity rapidly reaches a stable state after 30 min, and compared with a visible light probe, the near-infrared beta-galactosidase fluorescent probe has deeper tissue penetrating capacity, background interference can be effectively avoided, and the resolution ratio is improved; the compound can be applied to beta-galactosidase detection imaging and preparation of beta-galactosidase detection imaging reagents, especially breast cancer detection reagents.

The invention relates to the technical field of biological medicine, in particular to a TREM-1 targeted fluorescent molecular probe as well as a preparation method and application thereof, and discloses a fluorescent molecular probe named as' TREM-1 targeted fluorescent molecular probe as well as a preparation method and application thereof '. The invention aims to solve the problems of large molecular weight, poor pharmacokinetic performance, limited tissue penetration, high imaging background and poor imaging timeliness of the existing antibody fluorescent probe. The probe comprises a TREM-1 targeting module (TDM), a near-infrared fluorescence reporter group, a dark quenching group and a conformational sensitive connecting arm (CSL). The fluorescence of the probe is quenched before the probe is combined with TREM-1, and after the probe is combined with a target spot, TDM specific binding induces CSL conformation rearrangement, so that a reporter group and a quenching group are spatially separated, fluorescence resonance energy transfer (FRET) is relieved, and fluorescence activation is realized. According to the invention, rapid, high-contrast and low-background fluorescence imaging of atherosclerotic plaques is realized, and the probe is small in molecular size, strong in tissue penetrability and rapid in-vivo clearing.

The invention discloses a preparation method and application of near-infrared carbon dots capable of efficiently generating active oxygen and having targeting capacity, and belongs to the technical field of carbon nanomaterials. Compared with conventional blue-green light carbon dots, the near-infrared carbon dot provided by the invention has the advantages of large tissue penetration depth, small self-luminous interference of organisms, small damage to tissues and the like. The carbon dots provided by the invention can efficiently generate singlet oxygen, and have the capability of photodynamically killing tumor cells. Besides, abundant functional groups on the surfaces of the carbon dots are utilized, molecules capable of targeting organelles are modified on the surfaces of the carbon dots, so that the carbon dots can accurately position and target mitochondria, and compared with carbon dots without targeting capability, the carbon dots have stronger killing power on cancer cells.

The invention discloses a near-infrared photosensitizer for specifically recognizing fibroblast activation protease (FAP-alpha) as well as a preparation method and application of the near-infrared photosensitizer. A cyanine derivative is used as a fluorescent parent nucleus (ICyOH) of the photosensitizer, the fluorescence of the photosensitizer can be almost completely masked by modifying the photosensitizer, and after the photosensitizer reacts with FAP-alpha for 60 minutes, the fluorescence intensity is rapidly enhanced, so that high sensitivity, good selectivity and anti-interference performance are shown. The ZGP-LL-ICy is a photosensitizer with a cyanine structure, FAP-alpha fluorescence enhancement and high selectivity to FAP-alpha, the reagent shows high selective response to FAP-alpha enzyme, active ICyOH molecules with near-infrared fluorescence are released under the action of enzymolysis, and tumor fluorescence imaging of deep tissue penetration is achieved. Meanwhile, the activated ICyOH can generate cytotoxic active oxygen under the illumination condition, and photodynamic therapy on pancreatic cancer is achieved. The reagent has the advantages of low background fluorescence, deep tissue penetration, diagnosis and treatment integration and the like, and has important application value in the fields of early diagnosis and precise treatment of pancreatic cancer.

The invention belongs to the technical field of photosensitizers, and provides a near-infrared iridium complex, a preparation method thereof and application of the near-infrared iridium complex in preparation of a photodynamic therapy preparation. According to the near-infrared iridium complex, 1-(benzo [b] thiophene-2-yl) isoquinoline-4-formonitrile is introduced, and the introduction of cyano groups can significantly reduce an energy gap, so that the iridium complex emits red shift, and the tissue penetrating power is improved; meanwhile, a 2-(3, 5-dimethoxyphenyl)-1-phenyl-1H-imidazo [4, 5-f] [1, 10] phenanthroline ring structure is combined, and a 2-dimethoxyphenyl electron donating group is introduced, so that charge transfer of the near-infrared iridium complex is effectively promoted, the triplet-state service life of the complex is prolonged, and the capability of generating reactive oxygen species (ROS) of the near-infrared iridium complex can be effectively improved. The maximum absorption wavelength of the near-infrared iridium complex is 544 nm, the maximum emission wavelength of the near-infrared iridium complex is 720 nm, and the limitation of tissue penetration can be effectively overcome.

The invention relates to a nitroimidazole group meso-substituted heptamethine cyanine dye as well as a preparation method and application thereof. According to the dye, a nitroimidazole group is introduced into the middle position of a heptamethine cyanine parent nucleus, so that the dye has excellent absorption characteristics in a near-infrared region, the excitation wavelength is 730-780 nm, the emission wavelength is 800-860 nm, and the dye has good tissue penetrating power. The nitroimidazole group can effectively quench fluorescence in an excited state through a light-induced electron transfer effect, energy is promoted to be released in a non-radiative transition mode, the photothermal conversion efficiency is remarkably improved, and meanwhile high light stability and low fluorescence quantum yield are kept. The dye can be further prepared into a nano preparation and shows excellent biocompatibility and tumor targeting ability. The compound has wide application prospects in the fields of non-diagnostic or therapeutic protein labeling, bioimaging, photothermal therapy and the like, is especially suitable for preparing efficient photosensitive reagent drugs for tumor photothermal therapy, and provides a new strategy for design of near-infrared photothermal diagnosis and treatment materials.

This invention discloses a fluorescence-enhancing nanoparticle, its preparation method, and its applications, relating to the fields of organic fluorescent molecules and bioimaging technology. The nanoparticle comprises: NIR-II fluorescent molecules and an amphiphilic polymer encapsulating the NIR-II fluorescent molecules; the amphiphilic polymer has a hydrophilic segment and a rigid hydrophobic segment. The nanoparticle of this invention, based on "carrier microenvironment engineering," utilizes the rigid confinement and interfacial isolation provided by the amphiphilic polymer with the rigid hydrophobic segment for dual brightening, serving as a universal brightening platform applicable to various NIR-II fluorescent molecules. This nanoparticle can significantly improve aqueous phase quantum yield, enhance contrast and resolution in in vivo vascular imaging, improve deep tissue penetration, increase imaging sensitivity, and facilitate detection under low signal conditions. It also exhibits good stability and biocompatibility, supporting lesion imaging and intraoperative navigation applications.

The invention relates to the field of aggregation-induced emission photosensitizers, in particular to a mitochondria-targeted aggregation-induced emission photosensitizer as well as a preparation method and application thereof. The aggregation-induced emission photosensitizer disclosed by the invention is simple in synthetic route, is emitted in a near-infrared region, has deeper tissue penetration depth and excellent photodynamic activity, realizes specific killing of tumor cells through precise targeting mitochondria, triggers pyroptosis of cells and regulates and controls whole-body anti-tumor immune response.

The invention discloses an orange fluorescent silicon nanodot, a preparation method thereof and an application of the orange fluorescent silicon nanodot in mitochondrial imaging in living cells, the method comprises the following steps: S1, dissolving tetramethyl rhodamine methyl ester in ultrapure water, then adding N-(2-aminoethyl)-3-aminopropyltrimethoxysilane, uniformly stirring, and carrying out hydrothermal reaction on the obtained mixture; and S2, cooling after the reaction is finished, purifying the product by using a chromatographic column, eluting by using an eluent, collecting the eluent, and drying to obtain the orange fluorescent silicon nanodot. The silicon nanodot provided by the invention has the advantages of good light stability, high quantum yield and the like, and can be successfully applied to positioning imaging of mitochondria in living cells; compared with common blue-green fluorescence, orange fluorescence has better tissue penetrating ability and background interference resistance; the O-SiNDs prepared by the invention also has the property similar to that of lipophilic cations, and can realize high-specificity targeted marking on mitochondria of living cells.

The application discloses an anti-tumor nano self-assembled particle targeting mitochondria and a preparation method and application thereof. The nano self-assembled particle takes a photothermal agent as a core and takes an active oxygen donor reagent coated on the surface of the photothermal agent as a shell. The application utilizes the photothermal agent and the active oxygen donor to prepare the nano particle through self-assembly, realizes photodynamic therapy excited by near-infrared laser, has a deeper tissue penetration depth and causes less damage to normal tissues; the active oxygen is produced by breaking a peroxide bridge in artemether, and the limitation of traditional photodynamic therapy on anoxic conditions in a tumor microenvironment is overcome; the active oxygen is produced on a subcellular level by targeting mitochondria of tumor cells; and the nano particle has self-reporting fluorescence and can realize real-time monitoring of the in-vivo distribution of the nano particle.

The invention discloses an optical switch regulated and controlled pH response polymer and a preparation method and application thereof, the structural formula of the optical switch regulated and controlled pH response polymer is # imgabs0 #, and n1 and n2 are integers from 0 to 30; n3 is an integer from 8 to 50; a and b are integers from 1 to 100; and R is selected from one of # imgabs1 # imgabs2 #. According to the photoswitch regulated and controlled pH response polymer, self-assembly of the polymer is achieved through a coprecipitation method to form nano-micelles, the excellent biocompatibility is shown, the emission wavelength of 760 nm is achieved, the polymer is located in a visible light-near-infrared transition zone, the polymer is compatible with conventional visible light equipment, traditional visible light limitation is broken through, near-infrared level penetration and low interference are achieved, and the photoswitch regulated and controlled pH response polymer has good application prospects. And the background interference resistance and the tissue penetration depth are obviously improved. The micelle shows high-sensitivity fluorescence response to the pH value, accurate regulation and control can be achieved through illumination, and an efficient and accurate tool is provided for tumor diagnosis, drug delivery and real-time monitoring.

The invention discloses application of a clofazimine suspension as an NIR-II fluorescent tracer and a dye tracer in the field of auxiliary diagnosis and treatment. The preparation has the advantages of good biological safety (suitable for mothers and infants), high sensitivity, high tissue penetrability, light stability and the like, and can reduce the inconvenience of clinical multiple administration and improve the compliance of patients. The NIR-II fluorescence imaging capability overcomes the limitation of the traditional imaging in strong light or deep tissue'black box 'environment, and is more suitable for intraoperative navigation. Meanwhile, due to the inherent red dyeing characteristic, the dye can be used as a lymph dye tracer. In combination with the NIR fluorescence effect and the photoacoustic effect of the clofazimine suspension, the intraoperative lymphatic cleaning can be assisted through a multi-mode combined tracing technology.

An ablation instrument comprises a cable, a conductive antenna body coupled to the cable and configured to deliver ablative energy to tissue, and a tip having a cross-sectional diameter of less than 5 mm. The tip comprises a blade configured to cut a slit in the tissue. The blade comprises a plurality of cutting edges, and each cutting edge of the plurality of cutting edges has a width between 30% and 50% of the cross-sectional diameter of the tip.

The invention relates to a near-infrared band ratio type rare earth fluorescent nanoprobe as well as a preparation method and application thereof. The ratio-type rare earth fluorescent probe is a nanocrystal with a core-shell structure, an inner core layer of the ratio-type rare earth fluorescent probe is a light-emitting layer which absorbs laser radiation and emits a near-infrared fluorescent wave band, a middle shell layer of the ratio-type rare earth fluorescent probe is a light-emitting shell layer which absorbs laser radiation and emits another near-infrared fluorescent wave band, and an outer shell layer of the ratio-type rare earth fluorescent probe is an inert shell layer. Wherein the inner core layer takes Tm < 3 + > as a light-emitting center, and the light-emitting shell layer takes Er < 3 + > as a light-emitting center. Through difference response of two fluorescence signal wave bands to water absorption, an index relation between ratio-type fluorescence signal intensity and tissue penetration depth is established, and noninvasive depth positioning and imaging of a mouse living body are realized. The fluorescent nanoprobe can be used for depth positioning of blood vessels of the brain, the abdomen and the legs of a mouse and continuous monitoring of a dynamic migration process from the blood vessels to bones, and has a wide application prospect in the aspect of in-vivo ratio depth imaging.

The application discloses a near-infrared organic room-temperature phosphorescent material based on benzothiadiazole and a preparation method and application thereof, and belongs to the technical field of organic luminescent materials. The near-infrared organic room-temperature phosphorescent material based on benzothiadiazole can effectively inhibit the quenching effect of water and oxygen on phosphorescence, has high-brightness long-life water-phase room-temperature phosphorescence, and can realize high-brightness organic room-temperature phosphorescence in water phase. The application further provides a preparation method of the near-infrared organic room-temperature phosphorescent material based on benzothiadiazole, and has the advantages of simple process and low cost. The near-infrared organic room-temperature phosphorescent material based on benzothiadiazole shows excellent tissue penetration ability in a living body, realizes high-signal-to-noise ratio phosphorescence imaging of various organs in practical application, and has a good application prospect in the field of biological imaging.

The present invention belongs to the field of fluorescence microscopy imaging, and particularly relates to a hydrogel for expansion microscopy imaging, a preparation method thereof, and a sample expansion treatment method. The preparation method of the hydrogel includes preparing a hydrogel precursor solution and preparing a hydrogel prepolymer solution. The sample expansion treatment method includes sample gelation and sample expansion. The hydrogel of the present invention has excellent mechanical properties and high elongation at break. It has good stability, is convenient and safe to use. Moreover, by combining the traditional biomolecule anchoring step with tissue penetration and gelation, the hydrogel is used to expand mouse brain slices, and the mouse brain slices can be isotropically expanded to the maximum size after 48 h, obtaining a linear expansion multiple of about 4.0 times. The mouse brain slices embedded with DAAM-NMA-SA hydrogel and containing green fluorescent protein labels have significantly improved imaging effects in the expansion microscopy fluorescence images compared with the fluorescence images of the original brain slices, and the resolution has been greatly improved.

The invention relates to a water-soluble near-infrared two-region small molecular fluorescent compound as well as a preparation method and application thereof. The compound has a symmetrical conjugated skeleton structure as shown in a general formula (I), X and Y are independently O, S or Se, and R1-R7 are controllable hydrophilic or neutral substituents and can endow good water solubility and tissue penetrability. The compound has strong fluorescence emission in the wave band of 900-1500 nm, the quantum yield is higher than 0.5%, and the compound has excellent light stability and biocompatibility. The preparation method is simple, efficient and suitable for large-scale production. The compound can be widely applied to in-vivo imaging scenes such as kidney imaging, bladder metabolism monitoring, whole-body blood vessel and lymphatic system imaging, tumor tracing and intraoperative navigation, is discharged through urine after injection, is clear in metabolic pathway and low in biological accumulation risk, and has good in-vivo safety and clinical transformation potential.

The invention relates to the technical field of biomechanical luminescence diagnosis and treatment, in particular to a fusion protein and a reagent for ultrasonically regulating and controlling cell luminescence. A force response bioluminescent protein system composed of mechanical force sensitive ion channel (Msc) protein and jellyfish luminescent protein (AEQ) is constructed in cells, and transmembrane conduction of Ca < 2 + > under the action of ultrasonic mechanical force is achieved. And Ca < 2 + > is specifically combined with jellyfish luminescent protein, and the oxidized coelenterazine substrate emits light, so that the bioluminescence process regulated and controlled by ultrasound is achieved. The Ca < 2 + >-sensitive jellyfish luminescent protein is used as an ultrasonic mechanical force response bioluminescent carrier, and can generate instantaneous luminescence under ultrasonic control, so that the ultrasonic has the switch function characteristic of controlling the luminescence of the protein. Meanwhile, based on the non-invasiveness and excellent tissue penetrating capacity of ultrasound, accurate space-time positioning in the body can be achieved. Therefore, bioprotein luminescence triggered by ultrasound is not limited by treatment depth, and the technical bottleneck of in-vivo application of light therapy is broken through.

The application provides a near-infrared two-region fluorescent dye with mitochondrion targeting and aggregation-induced emission properties and application thereof in biological fluorescence imaging and tumor treatment. 41 H 37 F6N2PS, which has good light stability, outstanding mitochondrion targeting, clear ROS production mechanism and excellent photothermal performance. The substance has good mitochondrion targeting capacity and can destroy tumor mitochondria under laser irradiation of a certain intensity, thereby killing tumor cells. The aggregation-induced emission property makes it different from traditional aggregation quenching emission materials and has better biological imaging effect. The near-infrared property makes it have good tissue penetration capacity and biological fluorescence imaging effect. The substance has wide application prospects in near-infrared fluorescence imaging, preparation of mitochondrion targeting reagents and photodynamic therapy reagents. It can be applied to combined phototherapy of breast tumor PDT and PTT, and has simple synthesis steps and is conducive to commercial application and popularization.

The application discloses a kind of near-infrared fluorescent probe for detecting GSH and its preparation method and biological imaging application, belong to biological analysis technical field, the fluorescent probe NRh-BZ is by being decorated to oxygen heterocyclic skeleton with hemicyanine, increase conjugated structure, realize the detection of GSH in near-infrared light region, belong to off-on type fluorescent probe, with high selectivity, sensitivity and low cytotoxicity, its emission wavelength is 746nm, detection limit is as low as 46.8nM, can be applied to the detection imaging of GSH in living cell MCF-7 and rat liver tissue, and tissue penetration depth reaches 150 μm.The fluorescent probe NRh-BZ prepared in the application can quantitatively detect GSH in various biological systems.

The present invention provides a preparation method for a ruthenium-based covalent organic framework material, an antibacterial gel electrochemiluminescent device, and applications. The ruthenium-based covalent organic framework material, Ru@COFs, promotes the accumulation of tripropylamine co-reactants and accelerates the reaction rate with intermediates. The Ru@COFs emit stable, high-intensity near-infrared light near 630 nm, capable of deeper tissue penetration and reducing surface skin damage. The antibacterial gel electrochemiluminescent device utilizes a large-area light-emitting device combining flexible screen-printed electrodes with a double-network hydrogel. The nanoconfinement-enhanced near-infrared electrochemiluminescence of the Ru@COFs drives photodynamic therapy, achieving a deeper and safer antibacterial effect. The electrochemiluminescent device avoids the use of physical light sources, providing a model for the development of miniaturized, integrated wearable diagnostic and therapeutic devices.

The invention provides a composite glass cover plate and a preparation method and application thereof. The composite glass cover plate comprises a lower cover plate and a transparent glass substrate, the upper cover plate is arranged on the lower cover plate; the upper cover plate comprises a glass transparent area and an annular structural member embedded in the glass transparent area; the annular structural member penetrates through the upper and lower surfaces of the glass transparent area in the direction of the axis perpendicular to the surface of the glass transparent area; the annular structural member is blackened glass; the components of the glass transparent area, the annular structural member and the lower cover plate glass are the same; the glass transparent area, the annular structural part, the upper cover plate and the lower cover plate form an integral structure through vacuum hot pressing. The technical problem to be solved is how to prepare a composite glass cover plate, so that the composite glass cover plate can accurately control a light path transmitting and receiving angle and a total reflection critical angle, restrict light rays from entering skin in a fixed angle range, and improve the penetration depth of subcutaneous tissues. Meanwhile, compared with an ink product, the shading property is better, and the product strength is remarkably improved.

The invention discloses a near-infrared two-region AIE molecule with click activity for tumor treatment, and belongs to the technical field of aggregation-induced emission materials. The preparation method of the near-infrared two-zone AIE molecule with click activity comprises the following steps: dissolving a compound 1, a compound 2, tetrakis (triphenylphosphine) palladium and potassium carbonate in a mixed solution of tetrahydrofuran and water, and carrying out reflux reaction for 24 hours to obtain a compound 3; dissolving the compound 3 and the compound 4 in ethanol, and performing reflux reaction for 48 hours to obtain a compound 5; and dissolving the compound 5 and the compound 6 in dimethylformamide, and carrying out reflux reaction for 12 hours to obtain the near-infrared second-region AIE molecule. By constructing a strong donor-pi bridge-donor structure, a technical scheme of designing a near-infrared two-region AIE molecule with click activity is designed, a deeper tissue penetration depth can be realized, and meanwhile, the near-infrared two-region AIE molecule has excellent photodynamic activity, can play a good killing effect on cancer cells and does not influence normal cells.

The invention discloses an NIR-IIb response type up-conversion nanometer material, a hydrogen production nanometer reactor prepared from the NIR-IIb response type up-conversion nanometer material and application of the NIR-IIb response type up-conversion nanometer material, and belongs to the technical field of nanometer biomedical materials. The controllable hydrogen production nanoreactor is obtained by using a bionic carrier formed by lipidosome, a photosensitizer, a catalyst and a proton donor which are jointly wrapped in the bionic carrier, and an up-conversion nanomaterial prepared by a chemical synthesis method, a hydrothermal method and the like, and can generate hydrogen under the stimulation of light with specific wavelength. According to the nano-reactor provided by the invention, a proton donor is efficiently catalyzed to generate hydrogen, on-demand and controllable release of hydrogen at a focus part is realized by utilizing high-tissue-penetrability NIR-IIb stimulation, and the nano-reactor provided by the invention can effectively remove active oxygen at the focus part and relieve oxidative stress by controllably releasing the generated hydrogen, so that pulmonary fibrosis is effectively relieved. The nanoreactor provided by the invention has the advantages of good biocompatibility, high hydrogen production efficiency, strong targeting property, small treatment side effect and the like, and has a wide market application prospect.