27 results about "Tissue penetration" patented technology

The invention belongs to the technical field of nano biological materials, and particularly relates to a near-infrared two-region excitation and emission lanthanide nano fluorescent probe as well as a preparation method and application thereof. Lanthanide ions with near-infrared two-region absorption characteristics are used as sensitizers, nanocrystalline with a co-doped structure formed by the lanthanide ions and activator ions is used as a core, and a pure-phase matrix material shell layer which is the same as a core lattice is grown on the surface of a core particle in situ. The nano fluorescent probe can generate tunable near-infrared two-region fluorescence emission with the wavelength of 1000 to 3000 nm under the excitation of near-infrared light with the wavelength of more than 1000 nm. The near-infrared two-region excitation and emission characteristics of the nano fluorescent probe can provide higher tissue penetration depth and signal-to-noise ratio for in-vivo imaging. The nano fluorescent probe shows excellent performance in living body angiography, dynamic observation of intestinal tracts and multi-channel near-infrared two-region imaging, and has wide application prospects in the fields of biomedical detection, early diagnosis of diseases, surgical navigation and the like.

The invention discloses a near-infrared photosensitizer for specifically recognizing fibroblast activation protease (FAP-alpha) as well as a preparation method and application of the near-infrared photosensitizer. A cyanine derivative is used as a fluorescent parent nucleus (ICyOH) of the photosensitizer, the fluorescence of the photosensitizer can be almost completely masked by modifying the photosensitizer, and after the photosensitizer reacts with FAP-alpha for 60 minutes, the fluorescence intensity is rapidly enhanced, so that high sensitivity, good selectivity and anti-interference performance are shown. The ZGP-LL-ICy is a photosensitizer with a cyanine structure, FAP-alpha fluorescence enhancement and high selectivity to FAP-alpha, the reagent shows high selective response to FAP-alpha enzyme, active ICyOH molecules with near-infrared fluorescence are released under the action of enzymolysis, and tumor fluorescence imaging of deep tissue penetration is achieved. Meanwhile, the activated ICyOH can generate cytotoxic active oxygen under the illumination condition, and photodynamic therapy on pancreatic cancer is achieved. The reagent has the advantages of low background fluorescence, deep tissue penetration, diagnosis and treatment integration and the like, and has important application value in the fields of early diagnosis and precise treatment of pancreatic cancer.

The invention relates to a nitroimidazole group meso-substituted heptamethine cyanine dye as well as a preparation method and application thereof. According to the dye, a nitroimidazole group is introduced into the middle position of a heptamethine cyanine parent nucleus, so that the dye has excellent absorption characteristics in a near-infrared region, the excitation wavelength is 730-780 nm, the emission wavelength is 800-860 nm, and the dye has good tissue penetrating power. The nitroimidazole group can effectively quench fluorescence in an excited state through a light-induced electron transfer effect, energy is promoted to be released in a non-radiative transition mode, the photothermal conversion efficiency is remarkably improved, and meanwhile high light stability and low fluorescence quantum yield are kept. The dye can be further prepared into a nano preparation and shows excellent biocompatibility and tumor targeting ability. The compound has wide application prospects in the fields of non-diagnostic or therapeutic protein labeling, bioimaging, photothermal therapy and the like, is especially suitable for preparing efficient photosensitive reagent drugs for tumor photothermal therapy, and provides a new strategy for design of near-infrared photothermal diagnosis and treatment materials.

This invention discloses a fluorescence-enhancing nanoparticle, its preparation method, and its applications, relating to the fields of organic fluorescent molecules and bioimaging technology. The nanoparticle comprises: NIR-II fluorescent molecules and an amphiphilic polymer encapsulating the NIR-II fluorescent molecules; the amphiphilic polymer has a hydrophilic segment and a rigid hydrophobic segment. The nanoparticle of this invention, based on "carrier microenvironment engineering," utilizes the rigid confinement and interfacial isolation provided by the amphiphilic polymer with the rigid hydrophobic segment for dual brightening, serving as a universal brightening platform applicable to various NIR-II fluorescent molecules. This nanoparticle can significantly improve aqueous phase quantum yield, enhance contrast and resolution in in vivo vascular imaging, improve deep tissue penetration, increase imaging sensitivity, and facilitate detection under low signal conditions. It also exhibits good stability and biocompatibility, supporting lesion imaging and intraoperative navigation applications.

The invention relates to the field of aggregation-induced emission photosensitizers, in particular to a mitochondria-targeted aggregation-induced emission photosensitizer as well as a preparation method and application thereof. The aggregation-induced emission photosensitizer disclosed by the invention is simple in synthetic route, is emitted in a near-infrared region, has deeper tissue penetration depth and excellent photodynamic activity, realizes specific killing of tumor cells through precise targeting mitochondria, triggers pyroptosis of cells and regulates and controls whole-body anti-tumor immune response.

The invention discloses an orange fluorescent silicon nanodot, a preparation method thereof and an application of the orange fluorescent silicon nanodot in mitochondrial imaging in living cells, the method comprises the following steps: S1, dissolving tetramethyl rhodamine methyl ester in ultrapure water, then adding N-(2-aminoethyl)-3-aminopropyltrimethoxysilane, uniformly stirring, and carrying out hydrothermal reaction on the obtained mixture; and S2, cooling after the reaction is finished, purifying the product by using a chromatographic column, eluting by using an eluent, collecting the eluent, and drying to obtain the orange fluorescent silicon nanodot. The silicon nanodot provided by the invention has the advantages of good light stability, high quantum yield and the like, and can be successfully applied to positioning imaging of mitochondria in living cells; compared with common blue-green fluorescence, orange fluorescence has better tissue penetrating ability and background interference resistance; the O-SiNDs prepared by the invention also has the property similar to that of lipophilic cations, and can realize high-specificity targeted marking on mitochondria of living cells.

The invention relates to a near-infrared band ratio type rare earth fluorescent nanoprobe as well as a preparation method and application thereof. The ratio-type rare earth fluorescent probe is a nanocrystal with a core-shell structure, an inner core layer of the ratio-type rare earth fluorescent probe is a light-emitting layer which absorbs laser radiation and emits a near-infrared fluorescent wave band, a middle shell layer of the ratio-type rare earth fluorescent probe is a light-emitting shell layer which absorbs laser radiation and emits another near-infrared fluorescent wave band, and an outer shell layer of the ratio-type rare earth fluorescent probe is an inert shell layer. Wherein the inner core layer takes Tm < 3 + > as a light-emitting center, and the light-emitting shell layer takes Er < 3 + > as a light-emitting center. Through difference response of two fluorescence signal wave bands to water absorption, an index relation between ratio-type fluorescence signal intensity and tissue penetration depth is established, and noninvasive depth positioning and imaging of a mouse living body are realized. The fluorescent nanoprobe can be used for depth positioning of blood vessels of the brain, the abdomen and the legs of a mouse and continuous monitoring of a dynamic migration process from the blood vessels to bones, and has a wide application prospect in the aspect of in-vivo ratio depth imaging.

The application provides a near-infrared two-region fluorescent dye with mitochondrion targeting and aggregation-induced emission properties and application thereof in biological fluorescence imaging and tumor treatment. 41 H 37 F6N2PS, which has good light stability, outstanding mitochondrion targeting, clear ROS production mechanism and excellent photothermal performance. The substance has good mitochondrion targeting capacity and can destroy tumor mitochondria under laser irradiation of a certain intensity, thereby killing tumor cells. The aggregation-induced emission property makes it different from traditional aggregation quenching emission materials and has better biological imaging effect. The near-infrared property makes it have good tissue penetration capacity and biological fluorescence imaging effect. The substance has wide application prospects in near-infrared fluorescence imaging, preparation of mitochondrion targeting reagents and photodynamic therapy reagents. It can be applied to combined phototherapy of breast tumor PDT and PTT, and has simple synthesis steps and is conducive to commercial application and popularization.

The application discloses a kind of near-infrared fluorescent probe for detecting GSH and its preparation method and biological imaging application, belong to biological analysis technical field, the fluorescent probe NRh-BZ is by being decorated to oxygen heterocyclic skeleton with hemicyanine, increase conjugated structure, realize the detection of GSH in near-infrared light region, belong to off-on type fluorescent probe, with high selectivity, sensitivity and low cytotoxicity, its emission wavelength is 746nm, detection limit is as low as 46.8nM, can be applied to the detection imaging of GSH in living cell MCF-7 and rat liver tissue, and tissue penetration depth reaches 150 μm.The fluorescent probe NRh-BZ prepared in the application can quantitatively detect GSH in various biological systems.

The invention discloses a near-infrared two-region AIE molecule with click activity for tumor treatment, and belongs to the technical field of aggregation-induced emission materials. The preparation method of the near-infrared two-zone AIE molecule with click activity comprises the following steps: dissolving a compound 1, a compound 2, tetrakis (triphenylphosphine) palladium and potassium carbonate in a mixed solution of tetrahydrofuran and water, and carrying out reflux reaction for 24 hours to obtain a compound 3; dissolving the compound 3 and the compound 4 in ethanol, and performing reflux reaction for 48 hours to obtain a compound 5; and dissolving the compound 5 and the compound 6 in dimethylformamide, and carrying out reflux reaction for 12 hours to obtain the near-infrared second-region AIE molecule. By constructing a strong donor-pi bridge-donor structure, a technical scheme of designing a near-infrared two-region AIE molecule with click activity is designed, a deeper tissue penetration depth can be realized, and meanwhile, the near-infrared two-region AIE molecule has excellent photodynamic activity, can play a good killing effect on cancer cells and does not influence normal cells.

The invention discloses an NIR-IIb response type up-conversion nanometer material, a hydrogen production nanometer reactor prepared from the NIR-IIb response type up-conversion nanometer material and application of the NIR-IIb response type up-conversion nanometer material, and belongs to the technical field of nanometer biomedical materials. The controllable hydrogen production nanoreactor is obtained by using a bionic carrier formed by lipidosome, a photosensitizer, a catalyst and a proton donor which are jointly wrapped in the bionic carrier, and an up-conversion nanomaterial prepared by a chemical synthesis method, a hydrothermal method and the like, and can generate hydrogen under the stimulation of light with specific wavelength. According to the nano-reactor provided by the invention, a proton donor is efficiently catalyzed to generate hydrogen, on-demand and controllable release of hydrogen at a focus part is realized by utilizing high-tissue-penetrability NIR-IIb stimulation, and the nano-reactor provided by the invention can effectively remove active oxygen at the focus part and relieve oxidative stress by controllably releasing the generated hydrogen, so that pulmonary fibrosis is effectively relieved. The nanoreactor provided by the invention has the advantages of good biocompatibility, high hydrogen production efficiency, strong targeting property, small treatment side effect and the like, and has a wide market application prospect.

The invention discloses a dye with up-conversion performance, and a synthesis method and application thereof. The dye generally has up-conversion performance, can emit fluorescent light (short wavelength) under irradiation of light (long wavelength) of 750-900 nm, and can avoid the problem of photobleaching caused by short wavelength excitation. Meanwhile, the dye has a high extinction coefficient and a high fluorescence quantum yield in an aqueous solvent and a polar protic solvent, so that the dye is very beneficial to fluorescence imaging, gene sequencing, biomacromolecule recognition and the like. Meanwhile, the upconversion mechanism also has deeper tissue penetrating ability, is beneficial to in-situ imaging and photodynamic therapy of deep pathological tissues of a living body, and has a very good application prospect.

A clofazimine-based pharmaceutical suspension and an application thereof, wherein a drug delivery system has near-infrared fluorescence, photoacoustic imaging effect and red dye characteristics for in vivo tracing, as well as a near-infrared photothermal effect for tumor treatment. The drug delivery system combines the near-infrared photothermal effect with the anti-tumor effect of the drug itself, and adopts a combination of phototherapy and traditional chemotherapy to achieve effective inhibition of tumor occurrence and development; in addition, by combining the near-infrared fluorescence effect and the photoacoustic effect, a multimodal combined tracing technique can be used to assist intraoperative lymph node dissection, and the drug can achieve long-term retention at the tumor site, overcoming the limitations of traditional imaging in "black box" environments of strong light or deep tissues, making it more suitable for intraoperative navigation; the system possesses good biosafety, high sensitivity, high tissue penetration and photostability, which can reduce the inconvenience of multiple clinical administrations and increase compliance.

A camera system integrated into the trocar. The camera system allows for a wide view of the internal surgical site and 3D mapping of fiducial markers during a laparoscopic procedure. Once inside the patient, the camera system is configured to deploy from a recessed position in the distal end of the trocar. In various aspects, the internal camera system is configured to keep the trocar port free for surgical instruments and provide surgical personnel with an expanded view of the surgical environment.

The application discloses a photothermal agent for treating allergic rhinitis and application of the photothermal agent in preparation of allergic rhinitis drugs. 5000 The compound 2 is prepared by reacting the compound 1 with diethylene glycol-2-bromoethyl methyl ether, then the prepared compound 2 and DSPE-mPEG 5000 are dissolved in tetrahydrofuran to perform self-assembly and form nanoparticles, the nanoparticles have a red shift of an absorption wavelength due to a strong electron-withdrawing effect of the pyridine salt group, promote intramolecular charge transfer, have deeper tissue penetration ability, can effectively reach a deep part of a nasal mucosa and treat deep inflammation; the RH@NPs prepared by the application have high efficient photothermal conversion performance and sensitivity of photodynamic therapy, so that PTT and PDT can be used in synergistic treatment of allergic rhinitis, and the treatment effect is greatly improved, and a new way for treating allergic rhinitis is opened up.

The present application provides a kind of estrogen receptor positive breast cancer fluorescent probe by parent nucleus dye nile blue derivative, estrogen receptor (ER) recognition group tamoxifen and alkane chain three parts constitute, the probe has the structure of general formula 1.The probe shows folded conformation in water environment, there is photoinduced electron transfer effect between nile blue derivative and tamoxifen, no obvious fluorescence signal;When the probe recognizes ER protein, the recognition group is combined with the binding site, the conformation of the probe changes into unfolded conformation, and strong fluorescence signal is shown.The excitation wavelength of the probe is about 630 nm, the emission wavelength is about 640-700nm, has stronger tissue penetration ability, lower tissue self-absorption and light scattering, can be targeted to breast cancer cell overexpressed ER protein, and specifically highlights estrogen receptor positive breast cancer.

A device for the destruction of viruses by means of complementary radiation, focused on weakening the lipid layer that covers some viruses, thus indirectly destroying it, this technique uses intense modulated light radiation, the main emitter being a 460 nm LED, with the technique of the invention, a secondary radiation is emitted in the 400-460 nm band, to achieve the weakening and destruction of the light, the lipid layer that covers viruses such as SARS-CoV-2, this light radiation is complemented with ultrasound pulses, completing the destruction of the virus internally, for this a technique is used that involves the emission by stratified quantum excitation, this technique uses a monochromatic LED to achieve a very high intensity multicolor emission, this emission is very controllable in terms of tissue penetration.

The invention discloses a high-photosynthetic-efficiency heterostructure rare earth nano fluorescent developer as well as a preparation method and application of the high-photosynthetic-efficiency heterostructure rare earth nano fluorescent developer. The rare earth nano fluorescence imaging agent has a core-shell-shell structure, the core of the core-shell-shell structure is a rare earth ion luminescent layer inner core NaYbF4: Er < 3 + >, Ce < 3 + > and Zn < 2 + >, the rare earth ion luminescent layer inner core is sequentially coated with a rare earth ion sensitizer shell layer NaYbF4: Er < 3 + >, Ce < 3 + > and M < 2 + > and a heterogeneous inert protective shell layer MF2, and M is selected from at least one of Ca, Sr and Ba. The rare earth nano fluorescence imaging agent can absorb exciting light in the wave band of 980 nm, can generate emitted light in the wave band of 400-700 nm in a visible region to 1400-1700 nm in a near-infrared IIb region, has the advantages of high biological tissue penetrability, weak self-fluorescence interference and the like, and is beneficial to the application of the rare earth nano fluorescence imaging agent in the aspects of real-time fluorescence imaging of deep tissue penetrating living organisms, tumor surgical navigation and the like.

The invention provides a tumor targeting nano-drug as well as a preparation method and application thereof. Comprising rare earth fluorescent nanoflowers, the nanoflowers are formed by aggregation of nanoparticles composed of crystal nucleuses and shell layers, the crystal nucleuses are rare earth fluorescent nanocrystals, the rare earth fluorescent nanocrystals are rare earth nanoparticles, and the shell layers are alkaline earth metal ion shell layers coating the surfaces of the crystal nucleuses; the surface of the nanoflower is partially or completely covered with a functional biomolecular layer. The nano-drug has a relatively large gap, a relatively large specific area, efficient luminescence and high targeting property; according to the present invention, the fluorescent material has good light emitting performance in the visible light region and the near-infrared light region, and particularly has strong light emitting performance in the near-infrared second region 1400-1700 nm wave band, such that the application of the fluorescent material in the deep tissue penetrating biological living body real-time fluorescence imaging can be easily achieved, and the living body medical image with the high resolution and the high signal-to-noise ratio can be obtained.

The invention discloses a near-infrared two-region aggregation-induced emission compound as well as a preparation method and application thereof in tumor imaging, and belongs to the technical field of aggregation-induced emission materials and tumor imaging. According to the invention, an electron donor 4-phenyl-4H-dithieno [3, 2-B: 2 ', 3'-D] pyrrole is introduced, and the 4-phenyl-4H-dithieno [3, 2-B: 2 ', 3'-D] pyrrole and a strong electron acceptor benzobis (thiadiazole) form a brand-new near-infrared two-region emission AIE molecule through a D-A-D symmetric structure. The near-infrared second-region aggregation-induced emission compound provided by the invention shows the absorption wavelength of a near-infrared first region and the emission wavelength of a near-infrared second region in a tetrahydrofuran solution, so that a deeper tissue penetration depth is realized. Besides, the compound also has good AIE characteristics in a tetrahydrofuran / water mixed system, has excellent light stability and high signal-to-noise ratio, and overcomes the defects that a traditional near-infrared two-region probe is prone to aggregation-induced quenching and insufficient in light stability.

The invention discloses a short-wave infrared fluorescence endoscopic imaging device and method, and the device is based on a finished near-infrared endoscope, and achieves the dual-light-path multiplexing of an original imaging channel and an illumination channel, namely, the imaging channel carries out white light imaging and exciting light scanning, and the illumination channel carries out white light illumination and short-wave infrared fluorescence collection. The excitation light source module is connected with the scanning module, the scanning module and the white light imaging module are connected with the endoscope through the dichroscope, the short wave infrared detection module and the white light illumination module are connected with the endoscope through the all-in-one optical fiber bundle, and the control and data acquisition module is electrically connected with the scanning module, the short wave infrared detection module and the white light imaging module. The structure of an endoscope body does not need to be transformed, the technical bottleneck that a finished endoscope cannot carry out short-wave infrared fluorescence imaging is solved, white light-short-wave infrared fluorescence dual-mode imaging depth fusion is achieved, scattering and autofluorescence interference can be effectively reduced, and the tissue penetration depth and the signal background ratio of endoscope imaging are improved.

An optogenetic system promoting the targeted homing of MSCs to a damaged liver, and a preparation method therefor. On the basis that VUR8 exhibits a dimer structure in a natural state and dissociates following UVB irradiation, an optogenetic system comprising light-controlled CXCR4-releasing plasmids which contain UVR8 and CXCR4 sequences and upconversion nanoparticles which can convert NIR having strong tissue penetration capability into UVB is developed, and the optogenetic system is used for implementing the overexpression of CXCR4 only on MSC cells in the liver region so as to promote the targeted homing effect thereof.

The invention discloses a LiNbO3-based material with enhanced red and high-penetration near-infrared mechanical luminescence properties as well as a preparation method and application thereof, and relates to the technical field of luminescent materials. The chemical formula is LiNbxTa (1-x) O3: 0.03 Pr, 0.01 Zn, and x is more than or equal to 0.5 and less than 1. By doping the Ta element, the local lattice distortion is regulated and controlled, the coordination environment of a Pr < 3 + > luminescence center is optimized, and the concentration quenching point of Pr < 3 + > is obviously delayed, so that Pr < 3 + > with higher concentration can be effectively activated without generating non-radiative cross relaxation enhancement, and synchronous enhancement of red and near-infrared mechanical luminescence is realized; the material has high brightness and deep tissue penetrating power, and a high-performance near-infrared mechanical luminescent material is provided for deep tissue biomechanical sensing.

The application discloses a dye with up-conversion performance, a synthesis method of the dye and application of the dye. The dye has up-conversion performance, can emit fluorescence (short wavelength) under irradiation of 750-900 nm light (long wavelength), and can avoid the problem of photo-bleaching caused by short wavelength excitation. Meanwhile, the dye has high extinction coefficient and fluorescence quantum yield in water-based solvents and polar protic solvents, and thus is very beneficial to fluorescence imaging, gene sequencing and biological macromolecule recognition and the like. Meanwhile, the up-conversion mechanism has deeper tissue penetration capacity, is beneficial to in-situ imaging and photodynamic therapy of deep pathological tissue of a living body, and has a good application prospect.

The present invention relates to a system and method for use in ultrasound imaging for the selective optimization of imaging parameters with respect to an anatomical region of interest. The system and method of the present invention provides selective adjustment of imaging parameters to achieve higher resolution and / or deeper penetration into tissue, thereby improving image quality and / or field of view in the selected region of interest. The system for providing selective image focusing comprises a console configured to be operably associated with and exchange data with an ultrasound imaging device.

The invention discloses a dye with up-conversion performance, and a synthesis method and application thereof. The dye generally has up-conversion performance, can emit fluorescent light (short wavelength) under irradiation of light (long wavelength) of 750-900 nm, and can avoid the problem of photobleaching caused by short wavelength excitation. Meanwhile, the dye has a high extinction coefficient and a high fluorescence quantum yield in an aqueous solvent and a polar protic solvent, so that the dye is very beneficial to fluorescence imaging, gene sequencing, biomacromolecule recognition and the like. Meanwhile, the upconversion mechanism also has deeper tissue penetrating ability, is beneficial to in-situ imaging and photodynamic therapy of deep pathological tissues of a living body, and has a very good application prospect.

This invention discloses a metal-based photosensitizer, a pharmaceutical composition, and its applications. The structure of the metal-based photosensitizer is shown in formula (I). This photosensitizer is molecularly designed based on a receptor-donor-receptor π-conjugated structure, and the chalcogen atoms of the receptor backbone molecule, the length of the receptor side chain, the length of the alkyl chain on the central nitrogen of the receptor, and the length of the donor have been adapted and optimized. Its nano-formulation has a maximum absorption peak in the near-infrared region, making it suitable for long-wavelength photodynamic therapy and photothermal therapy with strong tissue penetration. This metal-based photosensitizer not only has strong reactive oxygen species generation properties but also good biocompatibility and strong antitumor activity, showing excellent prospects for application in the preparation of tumor therapeutic drugs.