Preparation method and application of narrow-band-gap W18O49-X nanobrush Schottky junction antibacterial nanoparticles based on near-infrared light response

Abstract

The invention relates to a preparation method and application of narrow-band-gap W18O49-X nano brush Schottky junction antibacterial nanoparticles based on near-infrared light response. In order to solve the problems of low phototoxicity and low tissue penetrability caused by weak absorption of an existing W18O49-X near-infrared (NIR) region, the invention provides a preparation method and application of narrow-band-gap W18O49-X nano-brush Schottky junction antibacterial nanoparticles based on near-infrared light response, the nanoparticles have good antibacterial performance and biocompatibility, the preparation process is simple, the preparation cost is low, and the preparation method is suitable for industrial production. The W18O49-X coated Au Schottky junction is constructed by uniformly growing Au nanoparticles on the W18O49-X nano brush through in-situ reduction, so that the photothermal conversion efficiency is improved, and the generation of active oxygen is increased. The antibacterial rate of staphylococcus aureus and the antibacterial rate of escherichia coli reach 99% or above, the PTT and PDT synergistic antibacterial method can effectively avoid drug resistance of bacteria, and the mild photo-thermal effect can promote differentiation, proliferation and migration of cells and accelerate revascularization.

Description

Technical Field

[0001] The invention relates to a preparation method and application of antibacterial nanoparticles. Background Art

[0002] As the outermost organ of the human body, the skin is particularly vulnerable to injury while protecting against external risks. The accumulation of pathogens or microorganisms in wounds not only hinders wound healing, but also causes serious infectious diseases such as endocarditis, pneumonia, sepsis, etc. Antibiotics have played an important role in people's fight against bacterial infections and are still the first choice for treating bacterial infections. However, the long-term overuse of antibiotics has led to the emergence of drug-resistant bacteria. The emergence of "super bacteria" such as methicillin-resistant Staphylococcus aureus (MRSA) has led to the use of increasingly large doses of antibiotics, which undoubtedly increases the burden on patients. Therefore, in the face of the increasingly serious problem of bacterial resistance, it is urgent to find new low-toxic, high-efficiency, and broad-spectrum antibacterial methods. Phototherapy is considered to be a very promising antibacterial strategy because of its advantages such as being less likely to develop drug resistance, easy to operate, high efficiency, and non-invasive.

[0003] In recent years, with the rapid development of nanotechnology, nano-antibacterial strategies are considered to be a promising method, including nanomaterial drug delivery, photothermal therapy (PTT), chemodynamic therapy (CDT), photodynamic therapy (PDT), etc. Among them, photothermal therapy (PTT) and photodynamic therapy (PDT) have received widespread attention. PTT uses photothermal agents (PTAs) to convert near-infrared light (NIR) into ablative heat to kill bacteria through electron-phonon and phonon-phonon coupling interactions, while PDT uses photosensitizers (PSs) to generate reactive oxygen species (ROS), including hydroxyl radicals (·OH), hydrogen peroxide (H2O2), singlet oxygen ( 1 O2), superoxide anion radical (O 2- ), these cytotoxic oxidants react with proteins and DNA, causing bacterial damage. These therapies offer advantages such as being noninvasive, highly effective, easy to administer, and having minimal side effects. However, due to the inherent limitations of antimicrobial drugs and bacterial resistance, single-mode antimicrobial therapy often fails to eradicate bacterial infections. Therefore, synergistic antimicrobial strategies are particularly important in clinical treatment.

[0004] Among many functional semiconductors, W 18 O 49-X W is an excellent semiconductor with visible light response, which has been widely used in pollutant degradation, water oxidation and gas sensors. 18 O 49-X Research on nanomaterials in the field of wound repair is still relatively limited, mainly due to their optical response properties. 18 O 49-XIt has an oxygen defect structure (Ov), which can regulate the electronic structure and improve the visible light response ability. 18 O 49-X It still has good photocatalytic activity and can produce reactive oxygen species (ROS) under visible light irradiation, thus achieving non-antibiotic antibacterial properties. 18 O 49-X It has a strong localized surface plasmon resonance (LSPR), which functions similarly to plasmon noble metals (such as Au and Ag), which helps in photothermal antibacterial, regulating the wound microenvironment and promoting tissue repair. 18 O 49-X It has good chemical stability and biocompatibility. 18 O 49-X As a photocatalytic antibacterial material, it still has potential value in promoting wound healing. Future research can further optimize its band gap structure, oxygen defect content and nano-engineering to enhance its application potential in the biomedical field. However, in existing studies, W 18 O 49-X It mainly absorbs ultraviolet-visible light, but has weak absorption in the near-infrared (NIR) region. The phototoxicity of the light source and tissue penetration limit the W 18 O 49-X Application in photoresponsive antibacterial and photothermal therapy of skin tissue. Summary of the Invention

[0005] The present invention is to solve the existing 18 O 49-X The weak absorption in the near-infrared (NIR) region leads to phototoxicity and low tissue penetration. A narrow-bandgap W based on the NIR light response is proposed. 18 O 49-X The invention discloses a preparation method and application of nanobrush Schottky junction antibacterial nanoparticles. The nanoparticles have good antibacterial properties and biocompatibility, and the preparation process is simple.

[0006] The present invention is based on the narrow band gap W of near-infrared light response 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is carried out according to the following steps:

[0007] Step 1: Dissolve WCl6 in n-propanol, pour it into a hydrothermal reactor, perform hydrothermal reaction, centrifuge and dry to obtain W 18 O 49-X Nano brush;

[0008] The ratio of the mass of WCl6 to the volume of n-propanol is (0.4-0.6) g: (60-70) mL;

[0009] The temperature of the hydrothermal reaction is 200°C and the time is 12 to 24 hours;

[0010] Step 2: W obtained in step 1 18 O 49-X The nanobrush was dispersed in methanol, stirred evenly and then added to HAuCl4 methanol solution, followed by NaBH4 methanol solution. After the reaction, it was washed by centrifugation with ultrapure water and then dried to obtain W 18 O 49-X @Au nanoparticles;

[0011] The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of methanol is (0.1-0.2) g: (10-20) mL;

[0012] The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the HAuCl4 methanol solution is (0.1-0.2) g: (1-2) mL, and the concentration of the HAuCl4 methanol solution is 0.05-2 mol / L;

[0013] The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the NaBH4 methanol solution is (0.1-0.2) g: (1-2) mL, and the concentration of the NaBH4 methanol solution is 0.05-1 mol / L.

[0014] The beneficial effects of the present invention are:

[0015] 1. The present invention uses WCl6 as raw material and synthesizes a W with near infrared response capability through hydrothermal reaction. 18 O 49-X Nano brush, with the existing W 18 O 49-X Compared with nanoparticles, W 18 O 49-X The band gap of the nanobrush is smaller, thanks to the success of oxygen vacancy engineering. Oxygen vacancies will introduce defect states or intermediate energy levels in the band gap of the material. These energy levels are located between the conduction band and the valence band, which effectively reduces the energy required for electrons to transition from the valence band to the conduction band (i.e., the band gap), making it easier to be activated by 808nm near-infrared light. 18 O 49-X Uniform growth of Au nanoparticles on nanobrush to construct W 18 O 49-X @Au Schottky junction, which solves the problem of W 18 O 49-XThe problem of rapid recombination of photogenerated electron-hole pairs caused by the small band gap can be solved by improving the utilization rate of electron-hole pairs and avoiding the accumulation of Au nanoparticles, thereby greatly improving the photothermal conversion efficiency (PCE) and increasing the generation of active oxygen. 18 O 49-X @Au has good biocompatibility and ultimately promotes wound healing. This multimodal antibacterial application opens up a new path in the treatment of infections.

[0016] 2. W used in the present invention 18 O 49-X Nanobrush has the ability to respond to near-infrared light, which solves the existing W 18 O 49-X Nanoparticles have a narrow bandgap and cannot be excited by near-infrared light. Au nanoparticles also possess excellent photothermal and bactericidal properties and are widely used in the antibacterial field. The nanoparticles prepared by this invention exhibit excellent antibacterial effects, with inhibition rates exceeding 99% against Staphylococcus aureus and Escherichia coli. The synergistic antibacterial effect of PTT and PDT can effectively prevent bacterial resistance. The mild photothermal effect can also promote cell differentiation, proliferation, and migration, accelerating angiogenesis.

[0017] 3. The method of the present invention is simple to prepare, has mild conditions, low raw material cost, is easy to convert into products, is conducive to industrial-scale production, and no toxic or harmful substances are emitted during the preparation process, which is a green and pollution-free processing process. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 W in Example 1 18 O 49-X TEM images of

[0019] Figure 2 W in Example 1 18 O 49-X @TEM image of Au;

[0020] Figure 3 W in Example 1 18 O 49-X @TEM-EDS image of Au;

[0021] Figure 4 W in Example 1 18 O 49-X @HRTEM image of Au;

[0022] Figure 5 W in Example 1 18 O 49-X @Au photothermal temperature rise at different powers;

[0023] Figure 6 W in Example 1 18 O 49-X and W 18 O 49-X @Au photocurrent response diagram;

[0024] Figure 7 W in Example 1 18 O 49-X and W 18 O 49-X @Au's impedance diagram;

[0025] Figure 8 W in Example 1 18 O 49-X and W 18 O 49-X @Au’s linear voltammetric curve;

[0026] Figure 9 This is a diagram of the antibacterial effect of the nanoparticles in Example 1 (Staphylococcus aureus);

[0027] Figure 10 This is a diagram showing the antibacterial effect of the nanoparticles in Example 1 (Escherichia coli);

[0028] Figure 11 These are pictures of wound healing at different time points after nanoparticles were used to treat rat wounds in Example 1;

[0029] Figure 12 Statistical graph of wound healing areas at different time points after the nanoparticles were used to treat rat wounds in Example 1. DETAILED DESCRIPTION

[0030] The technical solution of the present invention is not limited to the specific implementation methods listed below, but also includes any reasonable combination of the specific implementation methods.

[0031] Specific embodiment 1: This embodiment is based on the narrow band gap W of near-infrared light response 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is carried out according to the following steps:

[0032] Step 1: Dissolve WCl6 in n-propanol, pour it into a hydrothermal reactor, perform hydrothermal reaction, centrifuge and dry to obtain W 18 O 49-X Nano brush;

[0033] The ratio of the mass of WCl6 to the volume of n-propanol is (0.4-0.6) g: (60-70) mL;

[0034] The temperature of the hydrothermal reaction is 200°C and the time is 12 to 24 hours;

[0035] Step 2: W obtained in step 1 18 O 49-X The nanobrush was dispersed in methanol, stirred evenly and then added to HAuCl4 methanol solution, followed by NaBH4 methanol solution. After the reaction, it was washed by centrifugation with ultrapure water and then dried to obtain W 18 O 49-X @Au nanoparticles;

[0036] The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of methanol is (0.1-0.2) g: (10-20) mL;

[0037] The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the HAuCl4 methanol solution is (0.1-0.2) g: (1-2) mL, and the concentration of the HAuCl4 methanol solution is 0.05-2 mol / L;

[0038] The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the NaBH4 methanol solution is (0.1-0.2) g: (1-2) mL, and the concentration of the NaBH4 methanol solution is 0.05-1 mol / L.

[0039] This embodiment has the following beneficial effects:

[0040] 1. In this embodiment, WCl6 is used as raw material to synthesize a W with near-infrared response capability through hydrothermal reaction. 18 O 49-X Nano brush, with the existing W 18 O 49-X Compared with nanoparticles, W 18 O 49-X The band gap of the nanobrush is smaller, thanks to the success of oxygen vacancy engineering. Oxygen vacancies will introduce defect states or intermediate energy levels in the band gap of the material. These energy levels are located between the conduction band and the valence band, which effectively reduces the energy required for electrons to jump from the valence band to the conduction band (i.e., the band gap), making it easier to be activated by 808nm near-infrared light. 18 O 49-X Uniform growth of Au nanoparticles on nanobrush to construct W 18 O 49-X @Au Schottky junction, which solves the problem of W 18 O 49-XThe problem of rapid recombination of photogenerated electron-hole pairs caused by the small band gap can be solved by improving the utilization rate of electron-hole pairs and avoiding the accumulation of Au nanoparticles, thereby greatly improving the photothermal conversion efficiency (PCE) and increasing the generation of active oxygen. 18 O 49-X @Au has good biocompatibility and ultimately promotes wound healing. This multimodal antibacterial application opens up a new path in the treatment of infections.

[0041] 2. W used in this embodiment 18 O 49-X Nanobrush has the ability to respond to near-infrared light, which solves the existing W 18 O 49-X Nanoparticles have a narrow bandgap and cannot be excited by near-infrared light. Au nanoparticles also possess excellent photothermal and bactericidal properties and are widely used in the antibacterial field. The nanoparticles prepared in this embodiment exhibit excellent antibacterial efficacy, achieving over 99% inhibition against Staphylococcus aureus and Escherichia coli. The synergistic antibacterial effect of PTT and PDT effectively prevents bacterial resistance. The mild photothermal effect also promotes cell differentiation, proliferation, and migration, accelerating angiogenesis.

[0042] 3. The method of this embodiment is simple to prepare, with mild conditions, low raw material cost, easy product conversion, and is conducive to industrial-scale production. In addition, no toxic or harmful substances are discharged during the preparation process, and it is a green and pollution-free processing process.

[0043] Specific embodiment 2: This embodiment differs from specific embodiment 1 in that the drying temperature in step 1 is 40-60°C.

[0044] Specific embodiment three: This embodiment differs from specific embodiment one or two in that: the drying process in step two is: drying at 60° C. for 8 to 12 hours.

[0045] Specific embodiment 4: This embodiment differs from any one of specific embodiments 1 to 3 in that: after the reaction in step 2 is completed, the mixture is centrifuged and washed three times with ultrapure water.

[0046] Specific embodiment 5: This embodiment differs from any one of specific embodiments 1 to 4 in that: in step 1, the ratio of the mass of WCl6 to the volume of n-propanol is 0.6g:70mL.

[0047] Specific embodiment 6: This embodiment differs from any one of specific embodiments 1 to 5 in that: the temperature of the hydrothermal reaction in step 1 is 200° C. and the time is 24 hours.

[0048] Specific embodiment seven: This embodiment differs from any one of the specific embodiments one to six in that: Step two W 18O 49-X The ratio of the mass of the nanobrush to the volume of methanol was 0.2 g:10 mL.

[0049] Specific embodiment eight: This embodiment differs from any one of specific embodiments one to seven in that: Step two W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the HAuCl4 methanol solution was 0.2 g:1 mL, and the concentration of the HAuCl4 methanol solution was 0.05 mol / L.

[0050] Specific embodiment 9: This embodiment differs from any one of the specific embodiments 1 to 8 in that: Step 2 W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the NaBH4 methanol solution was 0.2 g:1 mL, and the concentration of the NaBH4 methanol solution was 0.05 mol / L.

[0051] Specific embodiment 10: This embodiment is based on the narrow band gap W of near-infrared light response 18 O 49-X Nanobrush Schottky junction antibacterial nanoparticles are used to prepare antibacterial dressings or antibacterial drugs that promote wound healing.

[0052] 1. In this embodiment, WCl6 is used as raw material to synthesize a W with near-infrared response capability through hydrothermal reaction. 18 O 49-X Nano brush, with the existing W 18 O 49-X Compared with nanoparticles, W 18 O 49-X The band gap of the nanobrush is smaller, thanks to the success of oxygen vacancy engineering. Oxygen vacancies will introduce defect states or intermediate energy levels in the band gap of the material. These energy levels are located between the conduction band and the valence band, which effectively reduces the energy required for electrons to jump from the valence band to the conduction band (i.e., the band gap), making it easier to be activated by 808nm near-infrared light. 18 O 49-X Uniform growth of Au nanoparticles on nanobrush to construct W 18 O 49-X @Au Schottky junction, which solves the problem of W 18 O 49-X The problem of rapid recombination of photogenerated electron-hole pairs caused by the small band gap can be solved by improving the utilization rate of electron-hole pairs and avoiding the accumulation of Au nanoparticles, thereby greatly improving the photothermal conversion efficiency (PCE) and increasing the generation of active oxygen. 18 O 49-X @Au has good biocompatibility and ultimately promotes wound healing. This multimodal antibacterial application opens up a new path in the treatment of infections.

[0053] 2. W used in this embodiment 18 O 49-X Nanobrush has the ability to respond to near-infrared light, which solves the existing W 18 O 49-X Nanoparticles have a narrow bandgap and cannot be excited by near-infrared light. Au nanoparticles also possess excellent photothermal and bactericidal properties and are widely used in the antibacterial field. The nanoparticles prepared in this embodiment exhibit excellent antibacterial efficacy, achieving over 99% inhibition against Staphylococcus aureus and Escherichia coli. The synergistic antibacterial effect of PTT and PDT effectively prevents bacterial resistance. The mild photothermal effect also promotes cell differentiation, proliferation, and migration, accelerating angiogenesis.

[0054] 3. The method of this embodiment is simple to prepare, with mild conditions, low raw material cost, easy product conversion, and is conducive to industrial-scale production. In addition, no toxic or harmful substances are discharged during the preparation process, and it is a green and pollution-free processing process.

[0055] Example 1:

[0056] This embodiment is based on the narrow band gap W of near-infrared light response. 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is carried out according to the following steps:

[0057] Step 1: Dissolve 0.6g WCl6 in 70mL n-propanol, pour into a hydrothermal reactor after dissolution, perform hydrothermal reaction at 200℃ for 24h, then centrifuge and dry at 60℃ to obtain W 18 O 49-X Nano brush;

[0058] Step 2: Take the 0.2gW obtained in step 1 18 O 49-X The nanobrush was dispersed in 10 mL of methanol, stirred evenly, and then added to 1 mL of 0.05 mol / L HAuCl4 methanol solution, followed by 1 mL of 0.05 mol / L NaBH4 methanol solution. After the reaction, it was washed three times by centrifugation with ultrapure water and then dried at 60 ° C for 12 h to obtain W 18 O 49-X @Au nanoparticles.

[0059] Figure 1 W in Example 1 18 O 49-X TEM images of Figure 2 W in Example 1 18 O 49-X @TEM image of Au. Figure 3 W in Example 1 18 O49-X TEM-EDS image of @Au shows that W was successfully prepared 18 O 49-X @Au.

[0060] Figure 4 W in Example 1 18 O 49-X HRTEM image of @Au reveals two different lattice spacings: 0.378nm corresponds to W 18 O 49-X The (010) plane of the Au nanoparticles is 0.23 nm, corresponding to the (111) plane of Au, confirming the successful loading of Au nanoparticles. Figure 5 W in Example 1 18 O 49-X @AuThe photothermal temperature rise at different powers shows that the nanoparticles have good photothermal response.

[0061] Figure 6 W in Example 1 18 O 49-X and W 18 O 49-X @Au photocurrent response diagram, Figure 7 W in Example 1 18 O 49-X and W 18 O 49-X @Au's impedance diagram, Figure 8 W in Example 1 18 O 49-X and W 18 O 49-X @Au's linear voltammetric curve. From these three figures, it can be seen that the loading of Au does improve the carrier separation ability.

[0062] Figure 9 This is the antibacterial effect diagram of the nanoparticles in Example 1 (Staphylococcus aureus), Figure 10 The antibacterial effect of nanoparticles in Example 1 (Escherichia coli); the control group (Control), the group without near infrared light W 18 O 49-X @Au Group (W 18 O 49-X @Au) and near-infrared light W 18 O 49-X @Au Group (W 18 O 49-X @Au+NIR); Figure 11 These are pictures of wound healing at different time points after nanoparticles were used to treat rat wounds in Example 1; Figure 12 Statistical graph of wound healing areas at different time points after the nanoparticles were used to treat rat wounds in Example 1. Figure 123M refers to the commercial dressing purchased, specifically 3M TM Tegaderm TM 1624w transparent dressing. The W prepared in this embodiment 18 O 49-X Au nanoparticles can achieve synergistic antibacterial effects of PTT and PDT, with a photothermal time of 10 min and an intensity of 700 mW / cm 2 Under these conditions, the antibacterial rate against Staphylococcus aureus and Escherichia coli reached more than 99% (the antibacterial rate against Staphylococcus aureus was 99.23%; the antibacterial rate against Escherichia coli was 99.15%); the wound healing rate could reach more than 98% after 14 days.

[0063] Example 2:

[0064] The difference between this embodiment and embodiment 1 is that the concentration of the HAuCl4 solution in step 2 is 0.1 mol / L. The other steps and process parameters are the same as those in embodiment 1.

[0065] The W prepared in this example 18 O 49-X @Au nanoparticles under photothermal conditions of 10 min and 700 mW / cm 2 Under the given conditions, the antibacterial rate against Staphylococcus aureus and Escherichia coli reached more than 99% (the antibacterial rate against Staphylococcus aureus was 99.33%; the antibacterial rate against Escherichia coli was 99.24%).

[0066] Example 3:

[0067] The difference between this embodiment and embodiment 1 is that the concentration of the HAuCl4 solution in step 4 is 0.15 mol / L. The other steps and process parameters are the same as those in embodiment 1.

[0068] The W prepared in this example 18 O 49-X @Au nanoparticles under photothermal conditions of 10 min and 600 mW / cm 2 Under the given conditions, the antibacterial rate against Staphylococcus aureus and Escherichia coli reached more than 99% (the antibacterial rate against Staphylococcus aureus was 99.24%; the antibacterial rate against Escherichia coli was 99.35%).

Claims

1. A narrow bandgap W based on near-infrared light response 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: Narrow bandgap W based on near-infrared photoresponse 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is carried out according to the following steps: Step 1: Dissolve WCl6 in n-propanol, pour it into a hydrothermal reactor, perform hydrothermal reaction, centrifuge and dry to obtain W 18 O 49-X Nano brush; The ratio of the mass of WCl6 to the volume of n-propanol is (0.4-0.6) g: (60-70) mL; The temperature of the hydrothermal reaction is 200°C and the time is 12 to 24 hours; Step 2: W obtained in step 1 18 O 49-X The nanobrush was dispersed in methanol, stirred evenly and then added to HAuCl4 methanol solution, followed by NaBH4 methanol solution. After the reaction, it was washed by centrifugation with ultrapure water and then dried to obtain W 18 O 49-X @Au nanoparticles; The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of methanol is (0.1-0.2) g: (10-20) mL; The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the HAuCl4 methanol solution is (0.1-0.2) g: (1-2) mL, and the concentration of the HAuCl4 methanol solution is 0.05-2 mol / L; The W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the NaBH4 methanol solution is (0.1-0.2) g: (1-2) mL, and the concentration of the NaBH4 methanol solution is 0.05-1 mol / L.

2. The narrow bandgap W based on near-infrared light response according to claim 1 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: The drying temperature in step 1 is 40-60°C.

3. The narrow bandgap W based on near-infrared light response according to claim 1 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: The drying process in step 2 is: drying at 60° C. for 8 to 12 hours.

4. The narrow bandgap W based on near-infrared light response according to claim 1 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: After the reaction in step 2 is completed, the mixture is centrifuged and washed three times with ultrapure water.

5. The narrow bandgap W based on near-infrared light response according to claim 1 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: The ratio of the mass of WCl6 described in step 1 to the volume of n-propanol is 0.6g:70mL.

6. The narrow bandgap W based on near-infrared light response according to claim 1 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: The temperature of the hydrothermal reaction in step 1 is 200° C. and the time is 24 h.

7. The narrow bandgap W based on near-infrared light response according to claim 1 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: Step 2 W 18 O 49-X The ratio of the mass of the nanobrush to the volume of methanol was 0.2 g:10 mL.

8. The narrow bandgap W based on near-infrared light response according to claim 1 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: Step 2 W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the HAuCl4 methanol solution was 0.2 g:1 mL, and the concentration of the HAuCl4 methanol solution was 0.05 mol / L.

9. The narrow bandgap W based on near-infrared light response according to claim 1 18 O 49-X The preparation method of nanobrush Schottky junction antibacterial nanoparticles is characterized by: Step 2 W 18 O 49-X The ratio of the mass of the nanobrush to the volume of the NaBH4 methanol solution was 0.2 g:1 mL, and the concentration of the NaBH4 methanol solution was 0.05 mol / L.

10. The narrow bandgap W based on near-infrared light response prepared as claimed in claim 1 18 O 49-X The application of nanobrush Schottky junction antibacterial nanoparticles is characterized by: Narrow bandgap W based on near-infrared photoresponse 18 O 49-X Nanobrush Schottky junction antibacterial nanoparticles are used to prepare antibacterial dressings or antibacterial drugs that promote wound healing.

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