1706 results about "Petri dish" patented technology

The utility model discloses a lycoris breeding device, and relates to the field of breeding devices, the lycoris breeding device comprises a box body, a bearing assembly, a breeding culture dish, a convection fan, a wind shield assembly, a spraying assembly, an LED fluorescent lamp and an electric heating pipe, the left side and the right side of the box body are provided with ventilation windows, and the convection fan is installed on one side of the ventilation windows through a supporting frame; the two wind shield assemblies are arranged on the left side and the right side of the box body respectively, the multiple bearing assemblies are arranged in an inner cavity of the box body in the mode that the positions of the bearing assemblies can be adjusted in the vertical direction, breeding petri dishes are placed in containing grooves formed in the tops of the bearing assemblies, and the multiple spraying assemblies and the LED fluorescent lamps are arranged at the top of the inner cavity of the box body and the bottoms of the bearing assemblies. The electric heating pipe is arranged at the bottom of the cavity of the box. The lycoris breeding device is ingenious in conception and convenient to use, enough living space is provided for breeding and growth of lycoris by adjusting the height distance between the bearing assemblies, meanwhile, the temperature of an inner cavity is rapidly adjusted and controlled, and the uniformity degree of the temperature is effectively improved.

The invention discloses a microgravity culture dish and a using method, and relates to the technical field of biological culture devices.The microgravity culture dish comprises a culture dish body and a culture dish cover, the culture dish body comprises a culture bottom shell and a sealing cover, a breathable separation ring is arranged in the middle of the culture bottom shell, and a culture area is arranged on the inner side of the breathable separation ring; a humidifying area is arranged on the outer side of the breathable separation ring, and a combined filter membrane is arranged on the inner side of the breathable separation ring; a culture medium channel is arranged on the side face of the culture bottom shell, a sealing plug is arranged on the culture medium channel, the culture bottom shell is provided with a gas exchange channel, and a water absorption body is arranged in the humidification area. Meanwhile, the invention discloses a using method based on the microgravity culture dish, a liquid dynamic exchange function and a gas exchange function are achieved at the same time in a suspension culture mode, cell development morphology observation and judgment can be effectively improved, and evaporation of a culture medium is inhibited in a humidification area. A partitioning mechanism is arranged, and culture of different regions is carried out according to the size of an actual sample.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The invention relates to a medicine, a medicine composition and application of the medicine and the medicine composition in treatment of liver fibrosis. The medicine and the medicine composition comprise an umbilical cord mesenchymal stem cell membrane; the umbilical cord mesenchymal stem cell membrane is prepared by the following steps: coating the surface of a temperature-sensitive culture dish with a matrix; adding the cell suspension of the umbilical cord mesenchymal stem cells into a temperature-sensitive culture dish for culturing; the temperature is reduced, the umbilical cord mesenchymal stem cells and the extracellular matrix secreted by the umbilical cord mesenchymal stem cells are separated in a lamellar mode, and the umbilical cord mesenchymal stem cell membrane is obtained. The medicine and the medicine composition provided by the invention can effectively relieve liver fibrosis.

The utility model discloses a citrus seedling cultivation device which comprises a base table and a rack fixedly connected to the top of the base table, a culture dish is fixedly connected to the top of the rack, a liftable placing plate is arranged in the culture dish, a first trapezoidal block is fixedly connected to the bottom of the placing plate, and a second trapezoidal block is fixedly connected to the bottom of the first trapezoidal block. The top of the base table is fixedly connected with an installation sliding rod. By arranging the liftable placing plate, citrus seedlings placed on the placing plate can be conveniently taken and placed, when the citrus seedlings are taken out, only a second trapezoidal block needs to be pulled by pulling a handle, and the inclined surface of the second trapezoidal block extrudes an extrusion roller, so that a first trapezoidal block can be upwards extruded; the placing plate can be jacked upwards, so that the placing plate can be jacked out of the culture dish conveniently, the citrus seedlings on the placing plate can be taken and placed conveniently, and the practicability of the citrus seedling cultivation device is improved.

The invention relates to a wound surface mixed bacteria unmixing method and system based on a double-branch attention mechanism, and belongs to the technical field of biomedical engineering and image processing. The method comprises the following steps: S1, acquiring hyperspectral images of a single strain culture dish, a blank culture dish and a mixed strain wound sample; s2, performing data extraction and preprocessing on the sample hyperspectral image, enhancing the data based on a spectral linear hybrid model and an adversarial generative network, and generating and synthesizing mixed bacteria hyperspectral data; s3, constructing a mixed bacteria unmixing model based on a space-spectrum double-branch attention mechanism; s4, inputting the synthesized mixed bacteria spectrum data into a mixed bacteria unmixing model for training, and optimizing parameters of the model according to the minimum total loss function of the model; and S5, inputting the hyperspectral data of the mixed bacteria wound sample to be unmixed into the trained mixed bacteria unmixing model, and outputting the unmixing abundance of the sample and the corresponding end metainformation.

The invention belongs to the field of functional material preparation, and particularly relates to a preparation method of a silicon-yttrium-carbon composite lithium ion battery negative electrode material, which comprises the following steps: (1) dispersing silicon nanoparticles, polyvinylpyrrolidone and yttrium salt in a deionized water solution to form a mixed solution, carrying out ultrasonic vibration dispersion, and then carrying out magnetic stirring in a room temperature air atmosphere; (2) transferring the obtained solution to a glass culture dish, and performing freeze drying in a freeze dryer; (3) transferring the precursor obtained by drying into a crucible, and placing the crucible in a tubular furnace for high-temperature calcination to obtain a silicon-yttrium-carbon composite material; and (4) grinding and mixing the obtained product with sodium alginate, conductive carbon black and deionized water respectively, uniformly coating the surface of a copper foil with the mixture, and then performing drying, slicing and tabletting treatment to obtain a target product. The preparation method is simple in synthesis process, good in electrode cycling stability, stable in structure and excellent in electrochemical performance.

The invention relates to the technical field of biology, and particularly discloses a device and a method for separating an adherent cell through a vortex sound field, an ultrasonic module, piezoelectric ceramics and an Archimedes spiral structure concave lens are arranged to generate the vortex sound field to act on a cell culture dish, so that an adjustable shear flow is generated on the cell culture dish, and the adherent cell is separated from the cell culture dish. And locally separating the adherent cells in the cell culture dish. According to the concave lens with the Archimedes spiral structure, the problem of defocusing of a high-order vortex sound field can be solved, and the falling control accuracy of adherent cells is improved. The proposed method does not require any physical contact with adherent cells, and does not require insertion of an instrument into a cell culture dish, thereby reducing the risk of contamination and cell damage. The stable flow generated by absorbing sound energy by the liquid is used for indirectly separating the adherent cells, the method is extremely low in energy density and has good biocompatibility, and the activity of the cells is ensured while the cells are efficiently separated.

The invention discloses a cell culture box convenient for taking and placing cell culture dishes, and relates to the technical field of cell culture, the cell culture box comprises a box body, the inner wall of the box body is fixedly connected with a plurality of placing plates, and the side walls of the top ends of the plurality of placing plates are provided with a plurality of first grooves. The inclination angle of the second clamping plate is the same as that of the outer wall of the corresponding cover plate, so that the cover plate is screwed above the cell culture dish by utilizing the second clamping plate, the primary installation between the cell culture dish and the cover plate is completed, and then a buckle on the outer side of the cover plate can be pressed into a clamping groove in the outer wall of the cell culture dish through a clamping block; when the cell culture dish needs to be taken out, a buckle is pulled out of a clamping groove in the outer wall of the cell culture dish by utilizing a pulling plate, and then the cover plate is taken down from the cell culture dish by utilizing a second clamping plate, so that the culture efficiency and reliability during cell culture are greatly improved.

The utility model provides a culture dish feeding device and a microbiological detection work station, and relates to the technical field of detection equipment. The culture dish feeding device comprises a feeding mechanism, a base, a rotating disc, a jacking mechanism and a culture dish frame, the rotating disc is rotatably mounted on the base and provided with a plurality of storage grooves, the culture dish frame is clamped in the storage grooves in a pluggable mode, a plurality of culture dishes can be stacked in the culture dish frame, through holes are formed in the groove wall of each storage groove, the jacking mechanism is fixed to the base, and the jacking mechanism is fixed to the base. The driving end of the jacking mechanism can penetrate through the through hole to abut against the culture dishes, and the feeding mechanism is used for transferring the culture dishes to the next station. Wherein the rotary table is provided with a material storage groove, a culture dish frame is placed in the material storage groove, a plurality of culture dishes can be stacked in the culture dish frame, along with rotation of the rotary table, the culture dish frame on which the culture dishes are stacked can be transferred to a target position one by one, and the culture dishes are jacked to a target height by virtue of the jacking mechanism, so that the feeding mechanism can pick up the culture dishes at a fixed point; and the feeding and picking efficiency of the culture dishes is improved.

The invention discloses a microbiological detection auxiliary device for culture dishes in a clean room and a use method thereof, the microbiological detection auxiliary device comprises: a lifting storage rack which can move in a vertical direction and is provided with a plurality of stacked and independently arranged placing trays in the vertical direction, and the placing trays are used for bearing culture dish bodies; the operation table is arranged on one side of the lifting storage rack; the multiple placing trays can be sequentially lifted to be located in the same horizontal plane with the operation table and attached to the operation table under the lifting power of the lifting storage frame. The transferring mechanism is used for transferring the culture dish body between the placing tray and the operation table; wherein a positioning cavity can be formed between the transfer mechanism and the operation table; the cover turning mechanism is arranged on the side, away from the lifting storage frame, of the operation table. By means of the arrangement, closed-loop control over storage, transfer, cover opening, detection and cover closing of the culture dishes is achieved, the artificial pollution risk is eliminated, meanwhile, the requirement for continuous detection can be met, and the purpose of improving the detection rate is achieved.

The invention discloses a seed germination ability detection device convenient for image acquisition, which comprises a detection box, an image acquisition part and a culture dish groove, an upward detachable spiral water and fertilizer box is arranged in the detection box, and the spiral water and fertilizer box is rotatably connected with the detection box; a plurality of culture dish grooves which are uniformly distributed in the spiral direction are formed in the spiral surface of the spiral water-fertilizer box, the bottoms of the culture dish grooves are communicated with an inner cavity of the spiral water-fertilizer box through water absorption parts, the culture dish grooves and the inner cavity of the spiral water-fertilizer box are in rotating and up-down sliding fit, and the spiral water-fertilizer box has the function of equivalently supplying liquid to each culture dish groove. According to the device, the spiral water-fertilizer box is arranged, then the spirally-distributed culture dish grooves are formed in the spiral water-fertilizer box, soil is placed in the culture dish grooves, the spiral water-fertilizer box inputs the same amount of water-fertilizer liquid into each culture dish groove, and the whole body formed by the spiral water-fertilizer box and the culture dish grooves can be taken out from the upper part of the detection box; the device has the advantage that the spiral water and fertilizer box and the culture dish can be independently cleaned and sterilized more conveniently and comprehensively.

The invention relates to the technical field of cell passage, and particularly discloses a cell passage device which comprises a pipetting mechanism, a material standby mechanism and an uncovering mechanism, the pipetting mechanism comprises a space mover and a pipetting assembly installed at the output end of the space mover, and the space mover is used for driving the pipetting assembly to move in the space; the material standby mechanism is used for placing test tubes, opening test tubes filled with liquid raw materials and opening standby test tubes; the cover opening machine is used for opening and closing covers of the culture dishes; the pipetting assembly comprises a pressing device, a rotating base and a plurality of pipetting guns, the rotating base and the pressing device are both installed at the output end of the space mover, the pipetting guns are distributed in the circumferential direction of the rotating base, the rotating base is used for rotating the pipetting guns to work stations, and the pressing device is used for pressing pressing heads of the pipetting guns located at the work stations to suck liquid or discharge the liquid; the pressing device is used for pressing a push head rod after the push head rod is aligned to the pipette to replace the pipette head; the pipetting mechanism further comprises a waste container located below the pipetting gun and a gun head set containing a plurality of gun heads of different specifications, the pipetting gun can be inserted into the gun heads of the gun head set to assemble new gun heads, and the gun heads or waste liquid pushed out of the pipetting gun can be received by the waste container. According to the scheme, the automatic operation of cell passage is realized, the automatic suction of the pipette and the automatic replacement of the pipette head can be realized in the pipetting process, and the control simplicity and convenience of the pipetting mechanism are ensured.

The invention discloses a bioreactor for stretching culture of artificial tendons, and relates to the technical field of bioreactors, the bioreactor comprises an incubator, a supporting plate and a rectangular sliding groove formed in the supporting plate, one side of the incubator is fixedly provided with a rotary swing mechanism, one end of the rotary swing mechanism is connected in series with a plurality of groups of stretching culture modules, and the stretching culture modules are arranged in the rectangular sliding groove. When a strip-shaped toothed plate slides in a reciprocating mode, a push-pull rod on a culture solution balancing mechanism and a piston assembly can be driven to move in a reciprocating mode in a cylinder through a connecting rod piece, and when the piston assembly moves in the cylinder in a reciprocating mode, culture solutions at all positions in a rectangular culture dish can be sucked into the cylinder to be mixed through a liquid suction pipe fitting; then the mixed culture solution in the cylinder can be circularly discharged into each part in the rectangular culture dish through the liquid outlet pipe fitting, so that the hydrodynamic environment in the reactor can be improved, and the influence on the stretching culture effect of the artificial tendon due to non-uniform deposition and distribution of the culture solution can be effectively avoided.

The utility model discloses a non-contact type cell co-culture device, which relates to the technical field of cell culture and comprises an upper-layer frame body and a lower-layer culture dish for arranging lower-layer cells, upper-layer cells planted on a climbing slide are arranged on the upper-layer frame body, the upper-layer frame body is arranged in the lower-layer culture dish in a lap joint mode, and the upper-layer cells and the lower-layer cells are arranged in a non-contact mode. The upper layer frame body comprises an upper surrounding support, a lower surrounding support and an inner side flange, the upper end of the lower surrounding support is connected with the lower end of the upper surrounding support, the lower end of the lower surrounding support is connected with the inner side flange, and an annular frame body with the hollow middle is formed; the inner side flange is arranged on the inner side edge of the bottom of the annular frame body; according to the cell co-culture device, double-layer cells can be effectively observed, and the double-layer cells can be efficiently utilized.

The invention discloses a rotary feeding device for culture dishes, relates to the technical field of automatic processing equipment for the culture dishes, and aims to solve the problems of frequent posture errors, low efficiency and need of shutdown for correction in an existing feeding mode. The device comprises a processing table, feeding conveying equipment, a culture dish feeding frame, detection equipment and a rotary feeding structure are arranged on the processing table, and rotary filling equipment is arranged on one side of the processing table; multiple sets of material taking adjusting assemblies, feeding assemblies and moving assemblies are distributed on the rotary feeding structure in the circumferential direction, the material taking adjusting assemblies can clamp the culture dishes subjected to laser detection and automatically correct the direction, the feeding assemblies receive the culture dishes through pneumatic suction cups and send the culture dishes to the rotary filling equipment, and the moving assemblies drive the culture dishes and the rotary filling equipment to move alternately. Automatic detection and correction of the postures of the culture dishes are achieved, the feeding efficiency is improved through cyclic operation of multiple sets of assemblies, shutdown is not needed, faults and cost are reduced, and the requirements of an automatic culture dish filling production line are met.

The invention discloses a method for detecting the antibacterial performance of a worsted fabric, and belongs to the technical field of cross fusion of textile material science and microbiological detection technology, and the method comprises the following steps: cutting a sample with a certain size and a regular shape from a to-be-detected worsted fabric, marking a plurality of different detection positions on the inner side and the outer side of the sample respectively, the method comprises the following steps: inoculating a test strain into a culture medium, placing a prepared sample in a sterile culture dish subjected to sterilization treatment, eluting each detection position marked on the inner and outer sides of the sample by using sterile normal saline, and calculating the antibacterial rates of different positions on the inner and outer sides of the sample by using a calculation formula, according to the detection method, the detection efficiency is improved, a multi-part result is obtained through a single process, and the antibacterial performance detection results of different positions on the inner side and the outer side of the worsted fabric can be obtained at the same time in the single detection process; the tedious process of single-side detection of the fabric or independent detection of the inner side and the outer side of the fabric in an existing method is avoided.

Platelet lysate compositions and cell culture media compositions for maintaining and / or growing mammalian cells, such as mammalian endothelial cells (ECs) and mammalian endothelial progenitor cells (EPCs), in particular human ECs (huECs) and human EPCs (huEPCs), such as primary huECs and primary huEPCs, are provided. The cell culture media compositions contain a basal medium, a platelet lysate and, optionally, one or more exogenously added growth factors. Also provided are methods for making and using such cell culture media compositions to grow and / or maintain ECs and EPCs, including huECs and huEPCs, as well as cell culture vessels, dishes, plates, and / or flasks pretreated with the cell culture media compositions.

An automatic end effector tip position initialization mechanism for a micromanipulation system is provided. The method includes: S1, positioning a macro end effector tip, calculating a camera pose by using ArUco Tag, and realizing a three-dimensional positioning of the end effector tip by using a triangulation method, and moving the end effector to a area near a center of a focal plane based on a visual servo; S2, positioning a micro end effector tip; S3, acquiring calibration data based on a visual servo, calibrating the end effector tip and the petri dish, and obtaining a left calibration matrix and a right calibration matrix reflecting the transformation relationship between left and right micromanipulator coordinates and image coordinates; generating an intuitive human-computer interaction interface based on a mouse and a keyboard according to acquired position information. The method has obvious advantages in terms of operation accuracy, operation efficiency and repeatability.

The utility model relates to an automatic transfer device for an electron irradiation biological experiment. The automatic transfer device comprises a lifting mechanism, a base is fixedly arranged on the lifting mechanism, a sliding block is arranged on the base in the length direction of the base, and a driving mechanism used for driving the sliding block to move is installed on one side of the base. A sliding table is fixedly arranged on the sliding block, a plurality of positioning grooves capable of fixing culture dishes are formed in the sliding table, a protective cover capable of being turned over is installed on the base, and a through hole matched with the positioning grooves is formed in the protective cover; the device is simple in structure, realizes automatic transfer of the culture dish, and improves the efficiency of an electron irradiation biological experiment; and compared with the traditional manual operation, the experiment time is greatly shortened.

The invention relates to the technical field of cell culture, in particular to a cell culture device which comprises a base, a culture box, a feeding mechanism, a sterilization mechanism and a sealing mechanism, a box body is mounted at the top end of the base, the culture box is mounted at one end of the interior of the box body, and the feeding mechanism comprises a culture frame and a moving assembly. The sterilization mechanism comprises a contact switch, a telescopic assembly, an abutting assembly and a plurality of ultraviolet lamps, the sealing mechanism comprises a first door opening, a first door plate, a second door opening, a second door plate, a lifting assembly and a driving assembly, and the first door plate and the second door plate can be rapidly switched, so that the incubator is prevented from being always opened in the process of taking out a culture dish; therefore, a large amount of external air enters the incubator, dust and bacteria carried in the air can be effectively prevented from entering the incubator, and the possibility that the incubator is polluted is reduced.

The invention relates to a bifunctional intercalation Ti3C2Tx composite photo-thermal material, an interface evaporator, a preparation method and application, PVDF is added into DMF for ultrasonic dispersion, then a Ti3C2Tx / carbon / cobalt composite photo-thermal material is added and continuously stirred until the Ti3C2Tx / carbon / cobalt composite photo-thermal material is uniformly dispersed, the obtained mixture stands, and bubbles are discharged; transferring the obtained black mixture into a glass culture dish, adding distilled water to immerse the black mixture to induce solidification, and forming a black blocky solid material; and after standing, soaking in distilled water, and forming the three-dimensional photo-thermal interface evaporator through a solvent exchange (DMF-water) process. An interface evaporator based on the Ti3C2Tx composite photo-thermal material has the evaporation rate of 2.00 kg.m <-2 >. H <-1 >, a photo-thermal runway prepared from the bifunctional intercalation Ti3C2Tx composite material shows excellent photo-thermal conversion efficiency, and snow can be rapidly melted and the surface of the runway can be dried under the simulation condition; the method has practical application value in solving the problems of water resource shortage and runway potential safety hazards.

Provided is a high-throughput microbe smart cloning and information analysis device, comprising a machine body, a strain feeding mechanism, a petri dish feeding mechanism, a lid opening mechanism, a lid opening and photographing mechanism, an inoculation mechanism, an inoculation needle replacement mechanism, a liquid level detection system, a pressure detection system, a high-performance computing module, and a control management system. The lid opening mechanism and the lid opening and photographing mechanism open the upper lids of strains and empty petri dishes that are transported to an inoculation position by the strain feeding mechanism and the petri dish feeding mechanism, respectively, and photograph the strains; the high-performance computing module receives image information, and locates and identifies the strains by means of a target detection algorithm; the inoculation mechanism moves an inoculation needle to inoculate the strains, while the liquid level detection system and the pressure detection system prevent the inoculation needle from pressing or damaging the petri dishes, and the inoculation needle replacement mechanism is used to replace the inoculation needle. By means of an automated device replacing manual operations to implement strain cloning actions, inoculation efficiency is improved, the safety of operators and strains is assured, and it is possible record and analyze strain information.

The invention provides a microorganism streak culture and result scanning device, and solves the problems of low automation degree, scattered device layout and unsmooth connection of all mechanisms of the existing microorganism treatment device. Comprising a workbench, a pretreatment system and a culture box are sequentially arranged on the surface of the workbench, and a culture frame is arranged in the culture box; a first turnover mechanism and a conveying mechanism are sequentially arranged between the pretreatment system and the culture frame, the first turnover mechanism clamps the culture dishes to turn over horizontally and conveys the culture dishes to the conveying mechanism, and the conveying mechanism conveys the culture dishes to the position below the culture frame; a pushing mechanism is arranged below the culture frame, and the pushing mechanism pushes the culture dishes into the culture frame; a taking-out and carrying mechanism and a scanning and storing system are sequentially arranged on the adjacent side of the incubator, and the taking-out and carrying mechanism takes out the culture dishes in the culture shelf and carries the culture dishes into the scanning and storing system; the scanning storage system comprises an active uncovering mechanism and a photographing and scanning mechanism, and the photographing and scanning mechanism is matched with the active uncovering mechanism to photograph and scan the interior of the culture dish.

The invention belongs to the technical field of cell culture, and particularly relates to a carbon dioxide incubator for cell culture, which comprises an incubator main body, an incubator door and a glass door are rotatably mounted on the incubator main body, the glass door is located between the incubator main body and the incubator door, an inner box is mounted in the incubator main body, and the incubator main body is provided with an outer box. A placing mechanism and a taking and placing mechanism are installed on the inner walls of the two sides of the inner box, carrying mechanisms which are arranged at equal intervals in a stacked mode are detachably installed on the placing mechanism, and circulating placing assemblies are detachably installed on the carrying mechanisms. The inside and outside of the inner box are separated through the glass door and the transfer mechanism, and in the process of taking out a culture dish containing a culture medium and when the detected culture dish is placed into the inner box again, through cooperation of the glass door and the transfer mechanism, the situation that the inner space and the outer space of the inner box are communicated is avoided, and the detection accuracy is improved. The culture medium in the culture dish which is always positioned in the inner box is not influenced by the external environment.

The utility model belongs to the technical field of automatic control, and particularly relates to an automatic pouring and inoculating workstation for laboratory microorganisms. The work station comprises a sample manipulator for grabbing a sample bottle to move; the weighing sensor module is used for weighing the sample bottle; the diluent filling mechanism is used for filling a diluent into the sample bottle; the vibrating and uniformly mixing mechanism is used for vibrating and uniformly mixing the sample; the pipetting module is used for driving a pipetting gun head to move and carrying out quantitative pipetting operation; the culture dish manipulator grabs the culture dish to move; the culture dish rotating movement mechanism drives the culture dish to rotate and move; the culture medium filling mechanism is used for quantitatively filling a culture medium into the culture dish; and the culture medium covering temporary storage mechanism is used for filling the culture medium into the culture dish for covering liquid covering treatment. The workstation can be used for automatically pouring and inoculating microorganisms, and the problem of low efficiency of microbiological inspection is solved.

The invention relates to a method for electrically activating oocytes based on nanosecond pulses. Single oocytes are sequentially and uniformly arranged between two parallel electrodes of an electrode dish, the electrode dish is characterized in that the distance between the electrodes is 1 mm, the electrodes are placed in a culture dish, and the two ends of the two electrodes are sealed; a culture solution is firstly put between the electrodes which are arranged in parallel, mineral oil is covered to prevent the culture solution from being evaporated, after preheating treatment, oocytes are sequentially and uniformly put between the two electrodes which are arranged in parallel one by one, and then nanosecond pulse electric activation treatment is applied. The electrodes which are arranged in parallel are adopted, the oocytes are sequentially and uniformly placed between the electrodes in a single mode, the situation that the electric pulse effect is reduced due to adhesion and stacking of all the cells is avoided, the oocytes receive uniform and equivalent electric pulse sequences, the action effect and stability of electric pulses on the cells are obviously improved, and the operation efficiency of the oocytes is improved. And the reliability and reproducibility of a test conclusion are improved.

The bacillus screening device comprises a culture box, a fixed seat is arranged on one side of the culture box, an openable box door is arranged at the top of the culture box, and a culture dish is arranged in the culture box; the culture dish is fixed in the culture box through the fixing bracket; the fixed bracket comprises a motor fixed in the incubator; the first rotating shaft is rotationally connected with the incubator and is driven by a motor to rotate; the fixing clamps are transversely fixed in the culture box at intervals, second clamping grooves are formed in the inner sides of the fixing clamps, and through holes which are through left and right are formed in the fixing clamps and used for allowing the first rotating shafts to penetrate through; the adjusting clamps are in sliding fit with the first rotating shaft through thread pairs and are symmetrical to the fixing clamps, and second clamping grooves are formed in the inner sides of the adjusting clamps and used for being matched with the first clamping grooves to fix the culture dishes; the adjusting assembly is arranged in the first clamping groove and the second clamping groove and used for adjusting the clamping distance. Culture dishes with different outer diameters can be fixed at the same time, so that the experiment efficiency is improved.

The invention relates to the technical field of aquatic animal cell culture, in particular to a rapid enrichment and separation method for gonad somatic cells of plectropomus leopardus. Comprising the following steps: selecting healthy plectropomus leopardus for anesthesia; the method comprises the following steps: taking gonad tissues, performing sterile treatment, cutting, centrifugal washing and filtering, inoculating the gonad tissues into a culture medium for primary culture to obtain gonad cells emigrated from tissue blocks, performing trypsin-EDTA digestion and centrifugal washing, and treating the gonad cells with 1 * PBS containing polysorbate; after closing, antibody incubation and washing, the marking effect is verified through a fluorescence microscope, and specific enrichment of gonad somatic cells is achieved; and filtering and purifying the enriched fluorescence-labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescence-positive cells under a fluorescence visual field, transferring the fluorescence-positive cells into a culture dish containing a complete medium, inoculating the separated fluorescence-positive cells into the optimized complete medium, and carrying out purification culture, so that the cells grow in a wall-attached manner and stable passage is realized.

The invention belongs to the technical field of medicine, and discloses a cuttlebone pattern mold and a preparation method thereof. The cuttlebone grain mold is prepared by a specific method comprising the following steps: filling a mixed rubber material of liquid silica gel mixed by a liquid A, a liquid B and the like according to a certain mass ratio into a culture dish of which the bottom is paved with an EDTA decalcified cuttlebone organic matter membrane, enabling S-shaped grains of the cuttlebone membrane to face upwards, removing bubbles by virtue of a vacuum bubble removing machine under the assistance of stirring, and performing vacuum drying to obtain the cuttlebone grain mold. Heating and curing temperature and time are controlled, cooling and molding are carried out, the mold is overturned for demolding, silica gel liquid of the same formula is poured for the second time, and the cuttlebone grain mold can be obtained after demolding. The cuttlebone texture mold has remarkable mechanical strength, hardness and high temperature resistance, remarkable osteogenesis performance and high transparency, can be used for preparing osteogenesis material textures, realizes accurate rubbing of cuttlebone textures, and has the advantages of low cost and good application performance.