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901 results about "Petri dish" patented technology
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A Petri dish (alternatively known as a Petri plate or cell-culture dish), named after the German bacteriologist Julius Richard Petri, is a shallow cylindrical glass or plastic lidded dish that biologists use to culture cells – such as bacteria – or small mosses.
Device and method for separating adherent cells from vortex sound field
PendingCN121495690ABioreactor/fermenter combinationsBiological substance pretreatmentsPetri dishAcoustic energy
The invention relates to the technical field of biology, and particularly discloses a device and a method for separating an adherent cell through a vortex sound field, an ultrasonic module, piezoelectric ceramics and an Archimedes spiral structure concave lens are arranged to generate the vortex sound field to act on a cell culture dish, so that an adjustable shear flow is generated on the cell culture dish, and the adherent cell is separated from the cell culture dish. And locally separating the adherent cells in the cell culture dish. According to the concave lens with the Archimedes spiral structure, the problem of defocusing of a high-order vortex sound field can be solved, and the falling control accuracy of adherent cells is improved. The proposed method does not require any physical contact with adherent cells, and does not require insertion of an instrument into a cell culture dish, thereby reducing the risk of contamination and cell damage. The stable flow generated by absorbing sound energy by the liquid is used for indirectly separating the adherent cells, the method is extremely low in energy density and has good biocompatibility, and the activity of the cells is ensured while the cells are efficiently separated.
Owner:ZHEJIANG UNIV
Culture dish feeding device and microbiological detection work station
ActiveCN223705561UBioreactor/fermenter combinationsBiological substance pretreatmentsComputer hardwareMicroorganism
The utility model provides a culture dish feeding device and a microbiological detection work station, and relates to the technical field of detection equipment. The culture dish feeding device comprises a feeding mechanism, a base, a rotating disc, a jacking mechanism and a culture dish frame, the rotating disc is rotatably mounted on the base and provided with a plurality of storage grooves, the culture dish frame is clamped in the storage grooves in a pluggable mode, a plurality of culture dishes can be stacked in the culture dish frame, through holes are formed in the groove wall of each storage groove, the jacking mechanism is fixed to the base, and the jacking mechanism is fixed to the base. The driving end of the jacking mechanism can penetrate through the through hole to abut against the culture dishes, and the feeding mechanism is used for transferring the culture dishes to the next station. Wherein the rotary table is provided with a material storage groove, a culture dish frame is placed in the material storage groove, a plurality of culture dishes can be stacked in the culture dish frame, along with rotation of the rotary table, the culture dish frame on which the culture dishes are stacked can be transferred to a target position one by one, and the culture dishes are jacked to a target height by virtue of the jacking mechanism, so that the feeding mechanism can pick up the culture dishes at a fixed point; and the feeding and picking efficiency of the culture dishes is improved.
Owner:MENGNIU DAIRY JINHUA CO LTD +1
Rotary feeding device for culture dishes
The invention discloses a rotary feeding device for culture dishes, relates to the technical field of automatic processing equipment for the culture dishes, and aims to solve the problems of frequent posture errors, low efficiency and need of shutdown for correction in an existing feeding mode. The device comprises a processing table, feeding conveying equipment, a culture dish feeding frame, detection equipment and a rotary feeding structure are arranged on the processing table, and rotary filling equipment is arranged on one side of the processing table; multiple sets of material taking adjusting assemblies, feeding assemblies and moving assemblies are distributed on the rotary feeding structure in the circumferential direction, the material taking adjusting assemblies can clamp the culture dishes subjected to laser detection and automatically correct the direction, the feeding assemblies receive the culture dishes through pneumatic suction cups and send the culture dishes to the rotary filling equipment, and the moving assemblies drive the culture dishes and the rotary filling equipment to move alternately. Automatic detection and correction of the postures of the culture dishes are achieved, the feeding efficiency is improved through cyclic operation of multiple sets of assemblies, shutdown is not needed, faults and cost are reduced, and the requirements of an automatic culture dish filling production line are met.
Owner:YANGZHOU YUEHAI MEDICAL TECHNOLOGY CO LTD
Automatic end effector tip position initialization mechanism for micromanipulation system
An automatic end effector tip position initialization mechanism for a micromanipulation system is provided. The method includes: S1, positioning a macro end effector tip, calculating a camera pose by using ArUco Tag, and realizing a three-dimensional positioning of the end effector tip by using a triangulation method, and moving the end effector to a area near a center of a focal plane based on a visual servo; S2, positioning a micro end effector tip; S3, acquiring calibration data based on a visual servo, calibrating the end effector tip and the petri dish, and obtaining a left calibration matrix and a right calibration matrix reflecting the transformation relationship between left and right micromanipulator coordinates and image coordinates; generating an intuitive human-computer interaction interface based on a mouse and a keyboard according to acquired position information. The method has obvious advantages in terms of operation accuracy, operation efficiency and repeatability.
Owner:ZHEJIANG UNIV
Microorganism streak culture and result scanning device
PendingCN121182606ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyMicroorganism
The invention provides a microorganism streak culture and result scanning device, and solves the problems of low automation degree, scattered device layout and unsmooth connection of all mechanisms of the existing microorganism treatment device. Comprising a workbench, a pretreatment system and a culture box are sequentially arranged on the surface of the workbench, and a culture frame is arranged in the culture box; a first turnover mechanism and a conveying mechanism are sequentially arranged between the pretreatment system and the culture frame, the first turnover mechanism clamps the culture dishes to turn over horizontally and conveys the culture dishes to the conveying mechanism, and the conveying mechanism conveys the culture dishes to the position below the culture frame; a pushing mechanism is arranged below the culture frame, and the pushing mechanism pushes the culture dishes into the culture frame; a taking-out and carrying mechanism and a scanning and storing system are sequentially arranged on the adjacent side of the incubator, and the taking-out and carrying mechanism takes out the culture dishes in the culture shelf and carries the culture dishes into the scanning and storing system; the scanning storage system comprises an active uncovering mechanism and a photographing and scanning mechanism, and the photographing and scanning mechanism is matched with the active uncovering mechanism to photograph and scan the interior of the culture dish.
Owner:SHANDONG LUZHEN MEDICAL TECH CO LTD
Rapid enrichment and separation method for gonad somatic cells of plectropomus leopardus
The invention relates to the technical field of aquatic animal cell culture, in particular to a rapid enrichment and separation method for gonad somatic cells of plectropomus leopardus. Comprising the following steps: selecting healthy plectropomus leopardus for anesthesia; the method comprises the following steps: taking gonad tissues, performing sterile treatment, cutting, centrifugal washing and filtering, inoculating the gonad tissues into a culture medium for primary culture to obtain gonad cells emigrated from tissue blocks, performing trypsin-EDTA digestion and centrifugal washing, and treating the gonad cells with 1 * PBS containing polysorbate; after closing, antibody incubation and washing, the marking effect is verified through a fluorescence microscope, and specific enrichment of gonad somatic cells is achieved; and filtering and purifying the enriched fluorescence-labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescence-positive cells under a fluorescence visual field, transferring the fluorescence-positive cells into a culture dish containing a complete medium, inoculating the separated fluorescence-positive cells into the optimized complete medium, and carrying out purification culture, so that the cells grow in a wall-attached manner and stable passage is realized.
Owner:HAINAN UNIVERSITY SANYA NANFAN RESEARCH INSTITUTE +1
Strain sampling body, transfer device and method
PendingCN121736878ABioreactor/fermenter combinationsBiological substance pretreatmentsPetri dishClassical mechanics
The invention relates to the technical field of biological experiments, in particular to a strain sampling body, a transfer device and a method. The strain sampling body comprises a connecting part and a sampling part, the connecting part drives the strain sampling body to move through a mechanical arm, and the sampling part is used for dipping strains on the surface from a culture medium and moving into a nutrient solution to sink into the bottom to form a growth channel; the strain transfer device comprises a culture dish, a deep hole plate and a mechanical arm. The invention also provides a strain sampling and transferring method. According to the invention, a surface contact mode is adopted, so that full contact with strains can be realized; the density is higher than the concentration of a nutrient solution, and a growth channel can be formed when sinking, so that a starting point is provided for strain growth, complete transfer of sampled strains is ensured, residual waste of the strains is avoided, and efficient and accurate sampling is realized; the strain sampling body has various forms, materials, surface states and the like; sampling and transferring tasks of strains are indirectly completed through the strain sampling body, and the requirements of different experiments for strain treatment are met.
Owner:QINGDAO GONGFA INTELLIGENT TECH CO LTD
Cell culture method
PendingCN121801799ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyPetri dish
The invention relates to the field of cells, in particular to a cell culture method which comprises the following steps: S1, taking out a cryopreservation tube from liquid nitrogen; s2, quickly putting the mixture into a water bath, and shaking the mixture from time to time to quickly melt the mixture; s3, wiping and disinfecting a cryopreservation tube with alcohol, opening a cover, injecting the cell suspension into a centrifugal tube by using a suction tube, dropwise adding a culture solution, and carrying out low-speed centrifugation; s4, pouring out the supernate, and adding a culture solution to suspend the cells; s5, sucking the cells into a culture dish filled with a culture solution, and slightly shaking all around, so that the cells in the culture dish are uniformly distributed; s6, placing the culture dish in an incubator at 37 DEG C for culturing; and S7, during culture, replacing a culture solution in the culture dish through a solution replacement mechanism arranged on the culture box in a closed state of the culture box. According to the invention, the culture solution of the culture dish can be replaced when the culture box is closed.
Owner:吕学凤
Automatic cell collecting device
ActiveCN224243105UBioreactor/fermenter combinationsBiological substance pretreatmentsProduction linePetri dish
The utility model belongs to the technical field of cell culture, and particularly relates to an automatic cell collecting device which comprises a rack and a sealing cover arranged on the rack, a normally closed butt joint window is arranged on one side of the sealing cover, and the butt joint window can be connected with a culture box; a culture dish can be placed in the culture box; the first mechanical arm is arranged on the operation platform; the liquid collecting mechanism is arranged on the operation platform, the liquid input mechanism is arranged on the operation platform, a first cover opening assembly is further arranged beside the liquid input mechanism, and the centrifugal collecting mechanism is arranged on the operation platform. By arranging the butt joint window, the first mechanical arm, the liquid collection mechanism, the liquid input mechanism and the centrifugal collection mechanism, the cell collection process can be fully automatically completed, the automation degree is high, the risk of artificial secondary pollution is reduced, meanwhile, a production line can be synthesized with a cell inoculation device, the first mechanical arm is shared, the size of the machine is reduced, and the production cost is reduced. The occupied area is small, the cost is low, and the structure is simpler.
Owner:DONGGUAN BOSHI INTELLIGENT CONTROL TECH CO LTD
Microorganism culture device for medical examination
PendingCN121227483ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyMicroorganism
The invention discloses a microbial culture device for medical examination, which comprises a cylinder body, a storage cavity is arranged at the upper end of the cylinder body, a cover body is arranged in the storage cavity, a through hole is formed in the cover body, a sealing cover is arranged at the through hole, a support frame and a lifting mechanism are arranged in the storage cavity, the support frame is used for placing a culture dish, and the lifting mechanism can push the culture dish out of the through hole. A liquid heating assembly is arranged at the bottom of the barrel, a first water channel is formed in the side wall of the storage cavity, a second water channel is formed in the cover, the liquid heating assembly communicates with one end of the first water channel, the other end of the first water channel communicates with one end of the second water channel, and the other end of the second water channel communicates with the heating assembly. The supporting frame, the lifting mechanism and the liquid heating assembly are all electrically connected to the control panel. The culture dishes on the supporting section frames extend out of the through holes with smaller openings through the lifting mechanisms, so that the heat loss can be reduced under the condition of water bath heating, and the temperature balance is ensured.
Owner:JINHUA VOCATIONAL TECH COLLEGE
A method for detecting microbial drug sensitivity
The application discloses a microbial drug sensitivity detection method. The application comprises: loading a microbial database, establishing a corresponding relationship between secondary microbial names and primary categories; loading a drug breakpoint database based on the "antimicrobial drug sensitivity experiment execution standard"; placing a standard culture dish in an image acquisition device to acquire an image, and calibrating an image analysis system with a vernier caliper; inputting sample information to be detected, acquiring and uploading the image; an image analysis system extracts the position of a drug sensitivity paper sheet, identifies the edge of a bacterial inhibition zone and calculates the diameter; the system extracts corresponding drug breakpoint thresholds based on the microbial category, compares the measured diameter and determines the sensitivity classification (S / I / R); and finally, the result is uploaded to a LIS system. The method can realize the automation, standardization and high efficiency of drug sensitivity detection.
Owner:GUIZHOU JINYU MEDICAL LAB CENT CO LTD
Stem cell preservation culture dish convenient to assemble in multiple layers
The utility model relates to a stem cell preservation petri dish convenient for multi-layer assembly, which comprises a placing tray, a petri dish body and a limiting mechanism, the petri dish body is detachably arranged in the placing tray, and the limiting mechanism used for limiting, fixing and quickly releasing the petri dish body is arranged in the placing tray. Four mounting seats at the bottom of one placing disc are inserted into four mounting columns at the top of another placing disc, multi-layer stacking of the multiple placing discs is achieved, when culture dish bodies in the placing discs need to be taken out, an abutting frame is firstly pulled outwards, the abutting frame is hinged to a moving rod, the moving rod can be pulled to move, two springs are arranged between the moving rod and the placing disc, and the moving rod is pulled to move; when the culture dish body is taken out, the moving rod moves to stretch the two springs until the abutting frame is separated from the placing disc, the abutting frame is rotated downwards, the abutting frame does not limit the culture dish body, at the moment, the abutting frame is not pulled any more, the moving rod is reset under the action of the two springs, the culture dish body can be taken out, and the culture dish body can be taken out rapidly.
Owner:SOUTH MEDICAL BIOLOGY (SHENZHEN) CO LTD
Culture device for improving saline-alkali soil through microbial agent
ActiveCN224047346UBioreactor/fermenter combinationsBiological substance pretreatmentsMicroorganismSoil science
The utility model belongs to the technical field of microbial agent culture, and particularly relates to a microbial agent improved saline-alkali soil culture device which comprises a culture box and a shaking assembly arranged in the culture box, the shaking assembly comprises a rotating disc, a transmission rod, a supporting plate, a stabilizing frame, a lap joint frame and a layered net frame, a transmission groove is formed in the bottom face of the transmission rod, and the supporting plate is arranged in the transmission groove. And the supporting plate is located on the upper side of the transmission rod, a rolling contact rolling wheel is arranged on the bottom face of the supporting plate, and the rolling wheel is rotationally installed on the upper side of the transmission rod. The culture dishes in the culture box can be shaken in different states through the shaking assembly, so that a culture solution is always kept in a dynamic mixing state, the dynamic environment of the saline-alkali soil is simulated, precipitation of saline-alkali components is avoided, the activity and reproduction efficiency of a microbial agent are improved, and the improvement effect of the microbial agent on the saline-alkali soil is enhanced.
Owner:SILK ROAD INNOVATION SMART AGRICULTURE RESEARCH INSTITUTE XINJIANG CO LTD
Culture dish moisturizing device
ActiveCN223805093UBioreactor/fermenter combinationsBiological substance pretreatmentsWater storagePetri dish
Owner:NANJING AIBEI BIOTECHNOLOGY CO LTD
Planting machine for landscaping
The utility model relates to the technical field of landscaping, in particular to a planting machine for landscaping, which comprises a frame, a frame, culture dishes and a storage tank, the frame is clamped and mounted on the outer wall of the upper end of the frame, the culture dishes distributed in a rectangular array are mounted on the inner wall of the frame, culture tanks are formed in the culture dishes, and the storage tank is connected with the culture dishes. A tray is arranged at the lower end of the interior of the culture groove, a clamping groove is formed in one side of the outer wall of the lower end of the tray, damping bearings which are distributed in parallel at equal intervals are installed on the two sides of the inner wall of the frame in an interference mode, and rotating shafts are installed in the damping bearings in an interference mode. And plants in the culture tank can be jacked up, so that workers can conveniently take out the plants, and the planting efficiency of the workers on the plants can be improved.
Owner:GUIZHOU YIHE DECORATION ENGINEERING CO LTD
Single cell biopsy and dynamic transcriptome tracking system and method
The application discloses a single-cell biopsy and dynamic transcriptome tracking system and method, and belongs to the technical field of single-cell sequencing. The system comprises the following steps: culturing single cells to be detected in a cell culture dish; puncturing the single cells by using an amino-modified quartz nanocapillary to extract trace cytoplasm samples; recovering the single cells in a culture environment; applying specific stimulation to the recovered single cells; at one or more time points after the stimulation is applied, puncturing the same single cells again by using the quartz nanocapillary to extract trace cytoplasm samples, and recovering the single cells after each extraction; and respectively performing reverse transcription, amplification, library construction and sequencing on the cytoplasm samples extracted at different time points to obtain dynamic transcriptome data of the single cells at different time points. The application can perform low-damage multiple longitudinal biopsies on the same single living cell, and can construct a dynamic transcriptome atlas of the single cell on a time axis in combination with downstream sequencing.
Owner:XIAMEN UNIV
Contact type bacteria culture dish
ActiveCN223805085UBioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyMicroorganism
The utility model discloses a contact type bacteria culture dish, and aims to provide a culture dish which has a larger contact area and is provided with an agar culture medium, and the key point of the technical scheme is that the culture dish comprises a dish body, a dish cover and a nutrient agar culture medium in the dish body, and is characterized in that the bottom of the dish body protrudes upwards; first net-shaped bulges for uniformly dividing the inner part of the dish body into a plurality of areas are arranged on the bottom surface of the inner side of the dish body. The culture dish is suitable for the field of microorganism devices.
Owner:SAINING (SUZHOU) BIOTECHNOLOGY CO LTD
A pollution-preventing bacterial culture device for a clinical laboratory
The application discloses a bacteria culture device with anti-pollution function for a clinical laboratory, which comprises a culture box and a feeding bin, the feeding bin is arranged on the outer wall of the culture box, and one end of the feeding bin extends to the inside of the culture box, a mechanical hand is arranged in the inside of the culture box on one side of the feeding bin, valve plates are arranged at both ends of the feeding bin, and sealing pads are arranged on the outer walls of the valve plates. The application realizes the taking and placing of the culture box under the isolation of the inside and outside of the culture box in a vacuum state, effectively prevents the external gas and microorganisms from entering the inside of the culture box, facilitates manual operation, realizes the batch arrangement and automatic conveying of the culture dishes, improves the culture efficiency, effectively prevents the culture dishes from moving accidentally when the nutrient solution or other operations are added through the linkage structure, facilitates the selective rotation of multiple culture dishes, is beneficial to the balanced absorption of the bacteria to the nutrients, meets the individual liquid adding requirements of different culture dishes, and improves the consistency and repeatability of the culture effect.
Owner:THE FIRST AFFILIATED HOSPITAL OF XIAN MEDICAL UNIV
A rapid detection device for pharmaceutical microorganisms
ActiveCN224337564UBioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyMicroorganism
This invention belongs to the field of microbial detection, specifically a rapid detection device for pharmaceutical microorganisms. It includes a detection chamber with a base fixedly installed at its bottom. A servo motor is fixedly installed in the middle of the base, and a rotating rod is fixedly installed at the output end of the servo motor. A turntable is fixedly installed at the top of the rotating rod, and placement slots are formed around the turntable. Multiple sets of steel balls are rotatably installed inside these slots, and protective rings are rotatably installed on the outer walls of the slots. This invention uses clamps to fix the culture dishes, preventing them from shifting off-center during rotation. It allows for the detection of multiple culture dishes simultaneously, facilitating control experiments and achieving a convenient and rapid result. It also avoids the problem of multiple handling of individual tests introducing more bacteria into the instrument. Furthermore, sterilization by ultraviolet light before detection greatly reduces the impact of bacteria on the detection process.
Owner:STADA PHARM (BEIJING) LTD
Microorganism culture device and regulation and control method thereof
PendingCN121136790ABioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyMicroorganism
The invention relates to the technical field of microorganism culture devices, and discloses a microorganism culture device and a regulation and control method thereof.The microorganism culture device comprises an incubator used for culturing microorganisms, a plurality of containing modules are installed in the incubator, each containing module comprises a containing table and a sliding table, and the containing tables are arranged in the incubator in a pluggable mode; the sliding table is slidably mounted on the inner wall of the incubator, a bearing table is rotatably connected to the upper surface of the placement table, an opening is formed in the surface of the bearing table and the surface of the placement table, a rotating column is arranged above the opening, and an illumination lamp is fixedly connected to the bottom of the sliding table; the upper end of the rotating column penetrates through the sliding table and is rotationally connected with the sliding table. According to the device, the lifting rotating assembly is matched with the placing module, so that the multi-layer culture dishes can be driven to rotate, microorganisms in the culture dishes are uniformly distributed, the uniform distribution effect of the microorganisms is realized, and the illumination lamp irradiates the culture dishes more uniformly.
Owner:NINGDE NORMAL UNIV
A culture medium for preparing fecal sulfur bacteria
ActiveCN224280234UBioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyPetri dish
This utility model relates to a culture medium for preparing fecal sulfur bacteria, belonging to the field of fecal sulfur bacteria preparation technology. It includes a box body, a door hinged to the outside of the box body, and two shelves located inside the box body. An adjustment mechanism for adjusting the distance between the two shelves is provided on the outside of the box body. Several storage trays are provided on the upper surface of each shelf. The adjustment mechanism includes a fixed frame fixedly connected to the outside of the box body, and a biaxial cylinder is fixedly installed inside the fixed frame. Connecting lugs are fixedly connected to both output ends of the biaxial cylinder. This fecal sulfur bacteria preparation culture medium allows for flexible adjustment of the distance between the two shelves by extending and retracting the output ends of the biaxial cylinder in the adjustment mechanism. It eliminates the need to change equipment due to different petri dish sizes, reducing the hassle of replacing devices due to mismatched petri dish sizes, improving experimental efficiency, and achieving the advantage of strong adaptability.
Owner:NANTONG JUYI CHENGGUANG BIOTECHNOLOGY CO LTD
Bioreactor for cell culture
ActiveCN223620402UBioreactor/fermenter combinationsBiological substance pretreatmentsPetri dishGear wheel
The utility model discloses a bioreactor for cell culture, and relates to the technical field of bioreactors, the bioreactor for cell culture comprises a support frame, the main body of the support frame is of an L-shaped structure, and the bioreactor is provided with a guide block fixedly connected to the bottom end surface of the support frame, through the arrangement of the guide block and the guide teeth, when the bracket is mounted on the guide seat, the guide block can be inserted into the inner position of the guide groove formed in the guide seat, the motor drives the gear to rotate, and the current culture dish can be quickly shaken through meshing transmission between the gear and the guide teeth; by arranging the mounting seat, the clamping push rod and the moving seat, when culture dishes of different specifications are clamped and positioned, the moving seat can be pushed towards the inner side by starting the clamping push rod mounted in the mounting plate; and rapid assembly is realized by clamping and limiting the culture dishes through the clamping plates fixedly connected to the inner sides of the moving seats.
Owner:KUNMING SMART BIOTECHNOLOGY CO LTD
Seed culture device for agricultural seedling culture
ActiveCN223758840UClimate change adaptationSelf-acting watering devicesAgricultural sciencePetri dish
The utility model discloses a seed culture device for agricultural seedling raising, which comprises an internally sealed cover body, and a water tank, a main bracket and an auxiliary bracket which are arranged in the cover body, a supporting frame plate for supporting a culture dish is horizontally and fixedly connected between the main bracket and the auxiliary bracket, and a water supply pipe is arranged right above the supporting frame plate; the water supply pipe is connected between the main support and the auxiliary support, dripping heads are arranged at the bottom of the water supply pipe at equal intervals, the water supply pipe is communicated with the water tank through a water pump, and a temperature sensor is arranged in the cover. The temperature and humidity inside the culture device can be accurately controlled, different water can be supplied to different types of seeds in a targeted mode, and a worker can conveniently observe the seed culture condition.
Owner:HUBEI XINMANPENG MODERN AGRICULTURAL TECHNOLOGY CO LTD
Mycobacterium tuberculosis drug resistance comparison detection device
ActiveCN223592719UBioreactor/fermenter combinationsBiological substance pretreatmentsPetri dishDrug resistance
The utility model relates to the technical field of sample detection, in particular to a tubercle bacillus drug resistance comparison detection device which comprises a bottom plate, a control panel fixedly connected to the front side of the bottom plate, a first sliding groove formed in the top end of the bottom plate, a fixed base arranged at the top end of the bottom plate, a culture dish in contact with the top end of the fixed base and a clamping block slidably connected to the fixed base. The electric push rod is controlled to be started by operating the control panel, the sliding block is driven to move on the inner side of the first sliding groove through stretching of the sliding rod of the electric push rod, and the electric push rod is driven to move on the inner side of the first sliding groove. The slide block moves to drive the culture dish arranged at the top end of the fixed base fixed by the slide block to move, and the moved culture dish is consistent with the previous culture dish in position, so that a user can directly observe the difference between different culture dishes, and the time consumed for adjusting the microscope is shortened.
Owner:中国人民解放军总医院第八医学中心
A method for constructing a hydrogel-based chip by 3D printing assisted water-phase frontal polymerization
The application discloses a method for constructing a hydrogel-based chip by 3D printing assisted water-phase front polymerization, and relates to the technical field of functional polymer material preparation; gelatin is dissolved in water to form uniform ink, a 3D printer is used to print channels and transfer the channels to a culture dish, and gelatin supports are arranged at two ends of the channels; methyl methacrylate gelatin monomers, acrylic acid monomers, a polymerization inhibitor, an initiator and a crosslinking agent are dissolved in water and injected into the culture dish; an electric iron is used to heat to 100 DEG C, and after the polymerization reaction is completed, the gelatin supports are removed, the channels are cleaned in water at 60 DEG C, and the hydrogel-based chip is obtained. The method has the advantages of high speed, high efficiency, low cost, fast molding and the like, provides a path for realizing the preparation of a complex hydrogel chip, and has high application value in the fields of biology and chemical engineering.
Owner:NANJING TECH UNIV
A test method for the resistance of carbodiimide anti-hydrolysis agent powder to phenolic yellowing and its application
This invention relates to a method for testing the resistance to phenolic yellowing of carbodiimide anti-hydrolysis agent powder and its application. The testing method includes: (1) grinding the sample to be tested and spreading it evenly in a petri dish; (2) placing 2,6-di-tert-butyl-p-cresol in a petri dish of the same size as in step (1); (3) placing the test reagent in a container; (4) placing the petri dish from step (1), the petri dish from step (2), and the container from step (3) into a sealed container for aging; (5) immediately using a colorimeter to detect the yellowing index of the sample after aging in step (4). The method of this invention can accurately detect powdered or crystalline carbodiimide anti-hydrolysis agents, solving the problem that it is currently impossible to accurately detect the phenolic yellowing resistance of powdered samples.
Owner:LANGYI NEW MATERIALS (YANTAI) CO LTD
Culture method for inducing high expression of umbilical cord mesenchymal stem cells (LRP1) and application
The invention discloses a culture method for inducing high expression of umbilical cord mesenchymal stem cells (LRP1) and application, and belongs to the technical field of biological medicines. The technical problem to be solved is to provide a standardized culture method for directionally inducing a specific functional phenotype cell population, so that efficient and accurate induction of LRP1 high-expression UCMSCs is realized, and clinical adaptation is met. According to the key point of the technical scheme, the culture method for inducing the LRP1 high-expression umbilical cord mesenchymal stem cells comprises the following steps: inoculating umbilical cord mesenchymal stem cells into a culture dish at the inoculation density of 5.5 * 10 < 3 > / cm < 2 > to 3.6 * 10 < 4 > / cm < 2 >, and culturing for 24-72 hours by using a special mesenchymal stem cell culture medium without adding a supplement, so that the acquisition efficiency of the LRP1 high-expression UCMSCs is improved, and the yield of the LRP1 high-expression UCMSCs is increased. Antibody labeling and flow sorting are not needed, so that the operation steps are simplified, and the complexity and cost of the culture process are reduced.
Owner:ANHUI TONGKE BIOTECHNOLOGY CO LTD
Multi-channel controllable pine wood nematode collection culture dish and culture collection method
PendingCN121667169AWithdrawing sample devicesAgriculture gas emission reductionBiotechnologyPetri dish
The invention relates to a multi-channel controllable pine wood nematode collection culture dish and a culture and collection method, and belongs to the technical field of pine wood nematode culture.The multi-channel controllable pine wood nematode collection culture dish comprises a culture dish body, the culture dish body comprises a culture dish cover and a culture dish body, the culture dish cover is provided with three sampling holes, sampling pipes are installed at the sampling holes, and the three sampling holes are communicated with the culture dish body. The three sampling tubes are annularly arranged along the center of the culture dish cover. According to the method, the natural enrichment phenomenon is utilized, the sampling pipe and the sample leaking hopper are matched, water drops containing the nematodes are collected through gravity, compared with a traditional filtering centrifugal collection method, the traditional time-consuming filtering step is omitted, the nematodes can be used after being extracted, the operation process is remarkably simplified, the nematode collection time is greatly shortened, the working efficiency is improved, and the method is suitable for popularization and application. And the three sampling pipes synchronously collect and continuously collect after the sampling pipes are replaced, so that the resource utilization rate is increased.
Owner:SHANDONG FOREST SCI RES INST
Preparation method and application of boxthorn leaf lignin synergistically modified bio-based preservative film
The invention discloses a preparation method and application of a boxthorn leaf lignin synergistically modified bio-based preservative film, boxthorn leaf lignin and a tea polyphenol loaded gamma-cyclodextrin metal organic framework are respectively mixed into carboxymethyl cellulose-gelatin composite gel, stirring is performed for 0.5 h, 20mL of different film forming solutions are poured into a polyethylene culture dish, and the boxthorn leaf lignin synergistically modified bio-based preservative film is obtained. And drying at 22 DEG C under the relative humidity of 35 + / -5% for 48 hours to obtain the boxthorn leaf lignin synergistically modified bio-based preservative film. The synergetic modified bio-based preservative film prepared by the invention can adjust the permeability of water vapor and gas in a fruit and vegetable fresh-keeping microenvironment, has better barrier property and antibacterial property, and can more effectively block external adverse factors, lock moisture and nutritional ingredients in fruits and vegetables, inhibit propagation of microorganisms and delay aging and decay of the fruits and vegetables; the shelf life of fruits and vegetables is effectively prolonged.
Owner:NINGXIA UNIVERSITY +1
A clinical microbiological examination drug sensitivity test report automatic generation device
The application relates to a clinical microbiological examination drug sensitivity test report automatic generation device, and belongs to the technical field of medical instruments. A fixing assembly is arranged in a box body to fix a culture dish at the center of the bottom of a cavity, so that a cavity top measuring assembly can be used to collect a drug sensitivity type label on the culture dish and measure the diameter of a bacteriostatic circle in the culture dish, the artificial measurement efficiency is improved, and the occurrence of false reports is avoided, the accuracy of drug sensitivity results is improved, and a disinfection and sterilization assembly is arranged in the box body, so that sample cross contamination and laboratory pollution are effectively avoided.
Owner:THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV