1951 results about "Petri dish" patented technology

A three-dimensional (3-D) culture “Petri-dish” for research in regenerative medicine, biotechnology and clinical translation is described. This 3-D perfusion culture dish is to advance in vitro culture tools from static 2-D to dynamic 3-D perfusion culture. Interwoven hollow fiber capillary membranes divide the “Petri-dish” culture space into a controllable 3-D pattern of different compartments, serving the functions of the organ's larger vasculature. These physically active scaffolds, which can be suitable for cell adhesion or cell aggregate immobilization, offer a supply of cells with high-performance mass exchange including gas supply and under perfusion conditions. In contrast to static and discontinuous medium supply, a dynamic culture can be achieved with continuous or alternating medium supply and integral oxygenation. They provide a more physiologic supply in the cell macro environment, including homeostasis of oxygen, pH, nutrition, soluble factors, and gradients of metabolites for the cells. Also, medium perfusion can be achieved. Consequently the invention was made for cultures at tissue density, especially stem cells and support cells, which strive to create their own stem cell niche.

A culture dish (1) comprising a basic structure (2) with a number of macro wells (4) for culturing oocytes and embryos, the culture dish further comprising a lid (3) with a movable part (8), the movable part (8) having an opening (11) adapted to the size of the macro wells (4), the movable part (8) being movable between a first position where all macro wells are closed by the lid (3) and further positions where for each further position (11) the opening (11) is aligned with one of the macro wells (4) to allow access to this macro well (4) through the opening (11) in the lid (3).

The present invention describes an automated platform for inoculating a variety of receptacles with biological samples for testing and analysis. The lab automation system includes a plurality of modules used to automate the inoculation of media for subsequent analysis. In this regard, the lab automation system has one module to enter specimen/order information and store an inventory of petri dishes. Another module is used to label the sample receptacles with a unique identifier that associates the receptacles with the sample. Yet another module includes a robot for retrieving sample and inoculating the receptacles. The sample inoculation module also includes an apparatus that will receive slides, inoculate those slides, and further process the slides for analysis. Finally, the lab automation system includes a module that streaks the culture media with the sample. Thus, the automated lab system described herein provides consistent samples with minimal input from a lab operator.

After supporting a petri dish S having a culture medium K contained therein with a given thickness on a petri dish holder 17 of a housing 14, a nozzle holder 13 is clamped at a top of the housing 14. When a motor 19 is energized to rotate a high static pressure fan 18, air is introduced through nozzle openings 12a, and flows through a space between the nozzle 15 and the culture medium K. By setting a distance between the nozzle and the culture medium to 0.5-1.5 mm, a high collection efficiency can be attained.

The invention discloses a method for preparing multistage-channel SAPO-n (n=34, 18) through solvent volatilization. According to the invention, a zeolite template agent is added into a mixed solution of an aluminum source, a silicon source, and a phosphorus source; stirring and a reaction are sufficiently carried out; the solution is completely delivered into a culture dish, and is air-dried into gel; under the effect of steam formed by water added into an autoclave, the gel is subjected to hydrothermal synthesis; powder converted from the gel is subjected to a heat treatment under high temperature, such that the multistage-channel SAPO-n zeolite is formed. The method provided by the invention has the advantages of simple process, easy control, easy realization, low cost, and environment friendliness.

When multiple kinds of bacterial colonies are present in a petri dish and, for example, a drug tolerance is to be measured, harvesting of mixed colonies of different types of bacteria makes it impossible to accurately determine the drug tolerance. Also, it is required to improve the throughput of a device for harvesting a bacterial colony. From images illuminated from multiple directions, isolating bacterial colonies are automatically extracted. Next, the image feature amounts are calculated from the multiple images that are illuminated from multiple directions and colonies are grouped depending on the feature amounts. Then, bacterial colonies to be harvested are determined based on the results of the grouping.

The invention relates to a method for testing cytotoxicity in full smoke contamination of cigarette. In the method, when full smoke exposure of the cigarette is carried out, an insertion type cell culture dish is used so that cultured cells are positioned at a gas-liquid interface between the smoke and the culture solution, and therefore, the purpose that the cells directly and fully contact withcigarette smoke is achieved, and the feel of the cells to the cigarette smoke can be comprehensively reflected. The method has high sensitivity, and the result can more truly reflect the cytotoxicityof the cigarette smoke. The insertion type cell culture dish is used so that a grain-phase part and a gas-phase part in the main-flow smoke of the cigarette directly and fully contact with the cultured cells, and when the full smoke exposure of the cigarette is carried out, the cells growing at microporous membrane attached to a wall are positioned at the gas-liquid interface between the cigarette smoke and the exposed culture solution, so that the problem that the cells can not comprehensively feel the full cigarette smoke in the previous experiments is solved; and meanwhile, in the method, the stationary step for formaldehyde stationary liquid is reduced so that the experimental process is simpler and more convenient.

The invention discloses a preparation method and a sensitivity regulation method of a pH type food freshness indication label. The preparation method comprises: extracting a pH sensitive natural dye to obtain a pH sensitive natural dye concentrated solution; dissolving a natural polymer material in a solvent to obtain a film-forming stock solution; sequentially adding a plasticizer, a water absorbent, a phase transfer catalyst and the pH sensitive natural dye concentrated solution into the film-forming stock solution, and fully stirring until the materials are uniformly mixed and dispersed toobtain a film-forming solution; and carrying out ultrasonic deaeration on the film-forming solution, slowly pouring the film-forming solution into a culture dish, and drying the solvent to obtain thefreshness indication label. According to the present invention, the pH value of the film-forming solution in the preparation process of the indication label is adjusted according to the front-back relationship between the freshness indication label color transition point and the food spoilage point, such that the two time points are matched so as to achieve the purpose of precise indication; and the method is simple and easy to implement, and the prepared indication label has high sensitivity, has the sensitivity capable of being regulated based on food spoilage rule, and has wide application.

Improvements to turntables that facilitate inoculation of petri dishes. In one embodiment, the present invention is a motorized turntable for a petri dish that allows the user to use both hands for inoculation of the petri dish. In another embodiment, the present invention features an improved upper plate for a petri dish turntable that allows the user to more easily remove the petri dish from the upper plate after inoculation. In another embodiment, the present invention provides an apparatus for rotating a conventional turntable for petri dishes.

The invention belongs to the field of environmental catalysis, and discloses a photocatalyst for efficiently degrading tetracycline under visible light and a preparation method and application of thephotocatalyst. The preparation method of the photocatalyst comprises the following steps: mixing a nitrogen-containing compound in water, carrying out hydrothermal treatment, cooling, transferring into a culture dish, and carrying out freeze drying treatment to obtain a precursor; and putting the precursor into a closed environment for high-temperature roasting, and naturally cooling to obtain thegraphite-phase carbon nitride photocatalyst for efficiently degrading tetracycline under visible light. According to the preparation method, the raw materials are mixed for hydro-thermal treatment and freeze-drying treatment to obtain the precursor, the graphite-phase carbon nitride obtained through constant-temperature roasting under different conditions has a relatively high specific surface area, and the separation of photo-induced electrons and holes can be effectively promoted through the formation of material defects; therefore, the purpose of efficiently degrading 10 mg/L tetracyclinehydrochloride under visible light is achieved.

A method for identifying rice blast resistance of rice comprises the steps of scissoring three rice stems in a large bract period from a rice growing field, bringing the rice stems back to a laboratory, scissoring 2cm from the upper portion of each stem section and 5-6cm from the lower portion of each stem section to obtain one rice stem with the whole length of approximate 7-8cm, putting the three rice stems into culture dishes with two layers of filter paper, adding sterile water of 1mL into each culture dish, washing cultivated rice blast fungus spores with the sterile water, obtaining concentration of 106 pcs/ml, dropping spore suspending liquid of 8 muL to stem section sites of each rice stem through a liquid moving gun, putting the culture dishes into a illuminating incubator at the temperature of 26 DEG C, wrapping the culture dishes with transparent films to keep humidity in the culture dishes, cultivating for ten days in light and dark alternating modes to survey disease conditions, dividing survey disease levels into three levels, regarding the first level as disease-resistant cultivars, regarding the second level as susceptible cultivars, and regarding the third level as highly susceptible cultivars. The method optimizes the concentration and inoculation amount of inoculated rice blast fungus spores and establishes simple, convenient and accurate grading standards.

A dispensing apparatus includes: a dish mounting portion having a mounting surface mounted with a dish having a bottom surface and a side surface surrounding the bottom surface; a syringe, arranged above the dish mounting portion, having a nozzle configured to discharge liquid toward an interior of the dish; and a first driving portion configured to change the syringe in direction with respect to a first axis as a center, wherein the first axis is orthogonal to a normal to the mounting surface of the dish mounting portion.

The invention provides a stem cell culture medium and a method for culturing endometrium stem cells by using the stem cell culture medium. The method comprises the following steps: separately collecting menstrual blood and endometrium tissues, respectively culturing the menstrual blood and endometrium tissues in the stem cell culture medium provided by the invention to respectively obtain menstrual blood adherent cells and endometrium adherent cells, culturing the menstrual blood adherent cells and endometrium adherent cells in a cell culture bottle, collecting the adherent cells by trypsinization, inoculating the adherent cells in a cell coculture dish, and culturing the adherent cells in the stem cell culture medium provided by the invention. The stem cell culture medium has the advantages of simple components, fewer added components and lower cost. After more than 20 generations of in-vitro culture, the cells can not easily have the phenomenon of aging or degeneration, and can maintain the activity and stem property of the stem cells for a long time. The stem cell culture method is simple and effective, the cell proliferation efficiency is high, and the in-vitro culture doubling time is only 20 hours or so. The cells can be stably amplified by 50 generations.

The disclosure relates to a device for dispensing a prescribed product in a Petri dish, the device comprising pistons, a transfer member with lid-bearing openings and a dish bottom, and a dispensor. According to the disclosure, the transfer member is a shuttle capable of translation relative to the base according to a direct reciprocating movement of the bearing openings between either of first and second stop positions, the dispensor being arranged so as to dispense the product above the inner opening and under the upper opening at the bottom of the dish when the openings are in a position selected from the first and second stop positions.

A lockable Petri dish is disclosed wherein the lid and base of the dish are provided with at least two pairs of locking members, each comprising a radial sheath and a sheath-engaging tab.

An automatic microbial inoculation instrument comprises a base and an instrument rear wall. The base and the instrument rear wall are mutually perpendicular to form an instrument supporting frame, a Petri dish control mechanism (3) and a sample delivery mechanism (4) are arranged on the base, and an inoculation ring moving mechanism (1) is arranged on the rear wall of the instrument. The automatic microbial inoculation instrument has the advantages that an inoculation method, namely a multi-region marking method which is accepted by most clinical microorganism personnel, is adopted, single strains can be separated more effectively, and sample misjudgment due to technical irregularity of clinical microorganism operators can be avoided; consumable cost in use of the instrument is equivalent to the cost of manual operations, so that accuracy of clinical sample inspection is guaranteed while enthusiasm in use of the instrument in hospital departments is improved; and the automatic microbial inoculation instrument enables hospital profits to be maximized in term of economic conditions, and single strain groups can be separated more accurately to enable more accuracy in detection by the automatic microbial inoculation instrument in term of medicine.

A preparing method of a sodium ion battery cathode material Na3V2(PO4)3/C is disclosed. The method includes 1) weighing a vanadium source, a sodium source, a phosphor source, a carbon source and a surfactant, dissolving into deionized water at the same time, and fully stirring to obtain a solution A, 2) heating and stirring the solution A, concentrating into sol, transferring the sol to a culture dish, freeze-drying at a temperature ranging from -40 DEG C to -55 DEG C for 5-12 h, and grinding to obtain a precursor, and 3) putting the precursor into a tube furnace with inert gas, pre-sintering at 300-400 DGE C for 3-5 h, heating to 700-850 DEG C, sintering for 8-24 h, and naturally cooling to obtain the Na3V2(PO4)3/C material. The method is simple and time-saving in steps. The prepared Na3V2(PO4)3/C material is uniform in particles, high in specific discharge capacity and good in rate capacity and cyclic performance.

The invention relates to a reconstruction method of tissue engineering human corneal epithelium. The method comprises the following steps of: adopting a DMEM/F12 culture medium containing 20% calf serum to carry out the in vitro culture on human corneal epithelium cells to a logarithmic growth phase, and adopting trypsin and a trypsin-EDTA digestion method to obtain a digestive amniotic carrier bracket of which the epithelium is completely removed; and after the digestive amniotic carrier bracket of which the epithelium is removed is flatly laid in a plug-in Petri dish and is firmly and pasted in a drying way, inoculating the human corneal epithelium cells at the logarithmic growth phase suspended in the DMEM/F12 culture medium containing IV type collagen and 20% calf serum to the plug-inPetri dish flatly laid with the digestive amniotic carrier bracket of which the epithelium is removed, and carrying out the in vitro reconstruction on the tissue engineering human corneal epithelium by a gas-liquid interface culture method. The invention has scientific and reasonable process, the reconstructed tissue engineering human corneal epithelium can be used for mass production, a lot of demands of vast blind patients with corneal epithelium diseases for the tissue engineering human corneal epithelium in clinical corneal transplantation treatment can be met, and the costs of the in vitro reconstruction and clinical treatment of the tissue engineering human corneal epithelium are low.

The invention discloses a method for breeding fly larvae and earthworms by kitchen garbage. The method is characterized in that the kitchen garbage is treated by the following steps: fermenting the kitchen garbage in a vacuum condition; regulating pH to 5-8; crushing, filtering and dewatering the kitchen garbage to obtain residual slag; adding 30%-50% of dried pig manure, dried cow manure, peanut straw powder, corn straw powder, other straw powder or mixture; putting the mixture to a fly larvae culture dish, paving the mixture in 5-10 thickness; adding fly eggs; maintaining the room temperature to be 30-35 DEG C and humidity to be 50-70% so that the fly eggs become fly larvae and then become maggots; after separating maggots, repeatedly performing the above steps by the residual slag till only fibrous material is remained in the residual slag; and adding the residual slag to the earthworm breeding base to breed earthworms. The method overcomes the problem that the utilization efficiency of the kitchen garbage after being treated by composting method is low, eliminates soil pollution, reduces air pollution and achieves recycling of the garbage.

The present invention discloses production process of multifunctional fermented liquid with bacterium, and is especially functional fermented liquid producing process with Rhodobacter sphaeroides. The Rhodobacter sphaeroides has preservation number of CGMCC No.1562. The production process includes separation from culture dish, strengthening of the bacterial seed in a shaking bottle, expanded culturing in the first seed tank (200L) and secondary seed tank (2000L) and large scale fermentation in large fermenting tank of 5000-20000 L.

A container assembly such as a petri dish or a contact plate for use as a sampling device for microorganisms includes a base member, a lid and a locking mechanism that provides a secure locking engagement between the lid and the base member. The locking mechanism is designed such that does not lock except upon application of a specific intentionally applied compressive force, and which may be readily disengaged from locking engagement without the need for a rotational movement or torsional force.

The invention relates to a method for preparing a carbon nano tube modified bipolar membrane with anion groups. The method comprises the following steps of: preparing a sodium carboxymethylcellulose (CMC) aqueous solution or a sodium alginate (SA) aqueous solution by using sodium carboxymethylcellulose or sodium alginate, adding a carbon nano tube with the anion groups, stirring and defoaming under reduced pressure to obtain viscous membrane liquid; casting the viscous membrane liquid in a smooth culture dish to prepare a cation exchange membrane; stirring and dissolving chitosan in 1 to 10 mass percent of acetic acid aqueous solution to prepare 1 to 10 mass percent of chitosan acetic acid aqueous solution, dripping 2.5 volume percent of glutaraldehyde solution slowly with stirring, and defoaming under the reduced pressure to obtain a chitosan (CS) anion membrane liquid; and forming a membrane of the CS anion membrane liquid, fixing the membrane to a prepared cation membrane layer, and airing at room temperature to obtain the bipolar membrane. Due to the adoption of the carbon nano tube modified cation membrane layer with the anion groups, the bipolar membrane has the characteristics of high water dissociation efficiency, small membrane impedance, low tank voltage, high compatibility of two membrane layers and the like.

The invention belongs to the field of use of a stem cell conditioned medium in medicinal cosmetics, and especially relates to an application of the conditioned medium in skin ageing resistance. Researches find that a large amount of complex active substances secreted by stem cells are stored in a medium in the in vivo culture process of mesenchymal stem cells, and is called as the stem cell conditioned medium, and an obtaining method of the medium comprises the following steps: carrying out digestive passage on p2 generation mesenchymal stem cells by using 0.05% pancreatin, inoculating 2*10<6> into a 150mm culture dish, and culturing to obtain p3 generation cells; and sucking the obtained culture supernatant when 80% of the p3 generation cells fuse, washing by using PBS twice, adding a new serum-free medium, putting the obtained cells in a 37DEG C 5% CO2 incubator, culturing for 48h, and collecting the obtained culture supernatant to obtain the stem cell conditioned medium. The stem cell conditioned medium obtained in the invention can supplement skin cell metabolism promotion substances lacked in skins and also can induce new collagen synthesis in order to maintain elasticity of skins and reduce wrinkles.