3133 results about "Petri dish" patented technology

The invention discloses an intelligent pretreatment workstation for microbiological detection, and relates to the technical field of microbiological detection. The module comprises a culture dish turntable, a transmission part, a homogeneous slapping part, a vortex oscillation part, a material rack storage part, a culture medium feeding part and the like; the culture dish turntable module comprises an upper dish station, a liquid injection station, an inoculation / coating station, a lower dish station and the like, as well as a transplanting electric module and a shaking-up module; the liquid injection unit can be used for injecting three culture solutions; the culture dish conveying module is responsible for conveying culture dishes to corresponding stations and is connected with an external feeding and receiving unit; the material rack storage module is used for storing pipettor tips, test tubes and homogenizing bags; the homogeneous slapping and vortex oscillation module is used for sample dilution and homogenization; the clamping robot is responsible for conveying the homogeneous bag and the test tube to the corresponding module and pulling out the plug of the test tube; the pipetting robot is used for sucking a sample and filling the sample into the culture dish. The steps from sample to culture can be completed at high speed and high efficiency, and the steps can be adjusted according to actual detection conditions through modular combination.

The invention discloses a stem cell culture device and method, and belongs to the field of stem cell culture. A stem cell culture device comprises a culture box and a germicidal lamp fixed to the top of an inner cavity of the culture box, the middle of the inner cavity of the culture box is rotationally connected with a rotating disc, the bottom of the rotating disc is fixedly connected with a U-shaped piece, the other end of the U-shaped piece is fixedly connected with a culture disc, the culture disc is attached to the inner wall of the culture box in a sliding mode, and a culture dish is installed at the top of the culture disc. An adsorption assembly for fixing a culture dish is arranged at the bottom of the culture tray; according to the device, a spraying pipe drives a spraying disc to rotate, so that a nutrient solution is sprayed out in a rotating manner along atomizing nozzles at all positions, and a culture disc can drive culture dishes at all positions to rotate in cooperation with the fitting friction effect between a friction wheel and a friction ring, so that the comprehensive and uniform spraying effect is achieved; the nutrient solution adding efficiency is improved, meanwhile, it is guaranteed that the quality of the nutrient solutions added into the culture dishes is the same, and therefore the culture effect is improved.

The invention discloses a strain culture dish positioning method and system based on monocular vision. The positioning method comprises the following steps: acquiring an edge image of a culture dish by a monocular vision camera, and presetting a grabbing point in the edge image; the edge image and the template image are matched based on an improved SIFT algorithm, and two-dimensional coordinates of the grabbing points are obtained; acquiring depth information of the culture dish by a laser triangulation ranging method, and combining the depth information with the two-dimensional coordinates to form three-dimensional coordinates; according to the coordinate system conversion relation between the camera and the manipulator, the three-dimensional coordinates are converted into a manipulator coordinate system, and the manipulator is controlled to grab the culture dish at the grabbing point. The defect that a traditional monocular vision system is difficult to obtain depth information is overcome, the improved SIFT algorithm is used for matching images and obtaining two-dimensional coordinates, the depth information of the system is obtained in combination with a laser triangulation ranging method, and therefore three-dimensional coordinate information of a target is obtained, the detection efficiency and positioning precision of the monocular vision system are improved, and the detection precision of the monocular vision system is improved. The method has the characteristics of accuracy and rapidness.

The invention belongs to the technical field of microorganisms, and particularly relates to a microorganism detection culture device and method.The system comprises an intelligent environment control system, and a sensor monitors the temperature, humidity and gas concentration of a culture environment in real time to ensure that environment parameters needed by microorganism growth are kept in the optimal state; the automatic sample processing and operating system avoids pollution or errors caused by manual intervention through a mechanical arm or an automatic transmission device; the real-time image monitoring and analyzing system is used for monitoring the growth state of the microorganisms in the culture dish; the camera can continuously shoot images in the culture process; the rapid detection module rapidly obtains activity information of microorganisms, rapid detection is achieved, and time needed by traditional culture is shortened. According to the invention, accurate control, automatic operation and rapid detection are realized, and an efficient, safe and intelligent solution is provided for microbial culture and detection.

The invention belongs to the technical field of bacterial culture, and discloses a high-throughput automatic monoclonal screening and picking workstation, which comprises a protective box for protection and isolation, and a workbench is arranged in the protective box; the plate stack module comprises a rotating plate stack arranged at the bottom end of the workbench, and a plurality of columns of culture dishes are arranged on the rotating plate stack in a lifting manner; the transfer module is arranged on the workbench and used for transferring culture dishes; the pipetting module comprises a pipetting assembly and a material storage assembly, and the pipetting assembly is used for transferring strains in the culture dish into the material storage assembly; the identification module is used for identifying and positioning the strains in the culture dish and then controlling the pipetting assembly to accurately transfer the strains. The device is simple in structure and flexible and convenient to use, high-throughput screening and picking of strains can be fully automatically carried out in a clean closed environment, manual intervention is not needed, the screening efficiency is high, strain pollution caused by manual intervention is avoided, the screening precision is high, the screening cost is reduced, and experiments are greatly facilitated.

The utility model discloses a lycoris breeding device, and relates to the field of breeding devices, the lycoris breeding device comprises a box body, a bearing assembly, a breeding culture dish, a convection fan, a wind shield assembly, a spraying assembly, an LED fluorescent lamp and an electric heating pipe, the left side and the right side of the box body are provided with ventilation windows, and the convection fan is installed on one side of the ventilation windows through a supporting frame; the two wind shield assemblies are arranged on the left side and the right side of the box body respectively, the multiple bearing assemblies are arranged in an inner cavity of the box body in the mode that the positions of the bearing assemblies can be adjusted in the vertical direction, breeding petri dishes are placed in containing grooves formed in the tops of the bearing assemblies, and the multiple spraying assemblies and the LED fluorescent lamps are arranged at the top of the inner cavity of the box body and the bottoms of the bearing assemblies. The electric heating pipe is arranged at the bottom of the cavity of the box. The lycoris breeding device is ingenious in conception and convenient to use, enough living space is provided for breeding and growth of lycoris by adjusting the height distance between the bearing assemblies, meanwhile, the temperature of an inner cavity is rapidly adjusted and controlled, and the uniformity degree of the temperature is effectively improved.

The invention provides auxiliary reproduction test tube infant fertilized egg culture equipment which comprises a constant-temperature bottom box and a sealing top box covering the top of the constant-temperature bottom box in a sealing mode, a culture dish cavity is formed in the middle of the top of the constant-temperature bottom box, and a variable culture dish with a variable culture plane is arranged in the culture dish cavity; compared with the prior art, when the fertilized eggs of the test tube baby are cultured, a proper amount of nutrient solution is put into the variable culture dish in a sterile laboratory, the fertilized eggs of the test tube baby are put into the nutrient solution to be cultured, and at the moment, the middle of the culture plane of the variable culture dish is concave downwards to be in a funnel shape; the test tube infant fertilized eggs are always located in the middle of the culture plane, cells start to split along with culture, the culture plane of the variable culture dish is gradually adjusted from a funnel shape to a plane shape along with the increase of the cells, and therefore the situation that the test tube infant fertilized eggs are excessively dispersed after being split is avoided, and stacking of multiple cells can be avoided; the observation condition is influenced.

The invention discloses a microgravity culture dish and a using method, and relates to the technical field of biological culture devices.The microgravity culture dish comprises a culture dish body and a culture dish cover, the culture dish body comprises a culture bottom shell and a sealing cover, a breathable separation ring is arranged in the middle of the culture bottom shell, and a culture area is arranged on the inner side of the breathable separation ring; a humidifying area is arranged on the outer side of the breathable separation ring, and a combined filter membrane is arranged on the inner side of the breathable separation ring; a culture medium channel is arranged on the side face of the culture bottom shell, a sealing plug is arranged on the culture medium channel, the culture bottom shell is provided with a gas exchange channel, and a water absorption body is arranged in the humidification area. Meanwhile, the invention discloses a using method based on the microgravity culture dish, a liquid dynamic exchange function and a gas exchange function are achieved at the same time in a suspension culture mode, cell development morphology observation and judgment can be effectively improved, and evaporation of a culture medium is inhibited in a humidification area. A partitioning mechanism is arranged, and culture of different regions is carried out according to the size of an actual sample.

The invention discloses stem cell culture equipment, and relates to the technical field of cell culture equipment, the stem cell culture equipment comprises a culture box, a rotating disc and a culture dish, horizontal plates are arranged in the middle of the culture box at equal intervals, and the middle of each horizontal plate is connected with the rotating disc through a bearing. The stem cell culture equipment is provided with a switching mechanism and an anti-deviation mechanism, a limiting ring and a circular track are separated, a conical block drives a tray to push culture dishes to be rapidly taken and placed, a rotating disc rotating slowly facilitates personnel to take or place the culture dishes one by one at the position close to an opening of a culture box, the limiting ring and the circular track are in matched butt joint, and the operation is convenient. The extrusion part elastically extrudes the culture dish on the tray when reversely moving and overturning, and meanwhile, the traction balance wheel is propped against the upper edge of a sealing end cover of the culture dish, so that the culture equipment can prevent the culture dish which is rotationally placed from shaking and shifting, and can be used for lifting and ejecting all the culture dishes at one time when the culture dish is taken out; and the operation of manually placing the culture dishes can also be reduced.

The invention discloses a preparation method of a colorimetric sensing array capable of intelligently detecting meat freshness, and belongs to the technical field of meat freshness intelligent detection systems. The preparation method of the colorimetric sensor array comprises the following steps: dissolving carboxymethyl cellulose in water to prepare an aqueous solution, then adding anthocyanin, then dividing the solution into N equal parts, taking 1 part as a blank, and respectively adding the rest N-1 parts into corresponding ionic liquids to form anthocyanin solutions with different color gamuts; the preparation method comprises the following steps: respectively casting in a culture dish, drying, uncovering a film, carrying out hydrophobic coating on the film by using a hydrophobic coating, drying, cutting into wafers with the same scale by using a puncher, and fixing in a pore plate to obtain the colorimetric sensor array. The colorimetric sensing array has the advantages of portability, visualization, no damage, stability, biodegradability, simplicity in preparation and operation, reusability and the like, can monitor and predict the freshness of meat in real time, and can solve the problems and defects of a traditional detection method.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The present invention discloses a preparation method of a rapid forming artificial blood vessel, comprising the following steps: under the condition of avoiding light, filling an annular cavity composed of an outer tube and an inner tube of a coaxial needle with a double-crosslinking photosensitive hydrogel and refrigerating at 4° C. for 8-10 minutes; filling the inner tube of the coaxial needle with CaCl2 aqueous solution; adjusting the temperature of the coaxial needle and a printing platform; under the action of ultraviolet light, adjusting extrusion speed of the outer tube and extrusion speed of the inner tube of the coaxial needle; printing extrudates in a sterile petri dish to obtain a rapid forming artificial blood vessel; and transferring the rapid forming artificial blood vessel to the sterile petri dish containing an ECM for culturing.

The invention discloses a verification method for regulating and controlling proliferation and differentiation of bovine adipocytes by gene interference, which comprises the following steps: cutting bovine adipocyte tissues into small blocks, directly pasting the small blocks in a culture dish for culture, and promoting cell growth and proliferation by using nutrition and growth factors of the tissues; a ZNF423 gene is targeted by adopting an siRNA technology, siRNA is transfected into bovine fat cells by utilizing a transfection reagent, and the siRNA is an interference fragment containing si-ZNF423; the method comprises the following steps: respectively extracting total RNA (Ribonucleic Acid) and total protein of bovine adipocytes, and detecting by utilizing qPCR (Quantitative Polymerase Chain Reaction) quantitative analysis, a WB (White Batch) technology, EdU dyeing, flow cytometry detection, oil red O, BODIPY (Boron Dipyrromethene), Nile Red dyeing, TG (Triglyceride) content determination and the like. According to the invention, ZNF423 gene expression is interfered by the interference fragment containing si-ZNF423, and the effect of inhibiting proliferation and differentiation of bovine adipocytes is detected by various methods, so that the effect of the interference fragment in the bovine adipocytes is known conveniently, and regulation and control of proliferation and differentiation of the bovine adipocytes are realized by interfering gene expression by the interference fragment. The beef quality can be improved; the beef cattle breeding can be optimized.

The invention relates to a medicine, a medicine composition and application of the medicine and the medicine composition in treatment of liver fibrosis. The medicine and the medicine composition comprise an umbilical cord mesenchymal stem cell membrane; the umbilical cord mesenchymal stem cell membrane is prepared by the following steps: coating the surface of a temperature-sensitive culture dish with a matrix; adding the cell suspension of the umbilical cord mesenchymal stem cells into a temperature-sensitive culture dish for culturing; the temperature is reduced, the umbilical cord mesenchymal stem cells and the extracellular matrix secreted by the umbilical cord mesenchymal stem cells are separated in a lamellar mode, and the umbilical cord mesenchymal stem cell membrane is obtained. The medicine and the medicine composition provided by the invention can effectively relieve liver fibrosis.

The utility model discloses a tumor cell culture device, which relates to the technical field of biological medicine, and comprises a culture box, the top of the culture box is provided with a box cover, a culture dish is arranged in the culture box, the bottom of the culture dish is provided with a heating plate, the top of the heating plate is provided with a fixing component for fixing the culture dish, and the bottom of the culture dish is provided with a fixing component for fixing the culture dish. A heating plate is arranged on the incubator body, a shaking assembly for shaking the heating plate is arranged on the heating plate, an adjusting assembly for adjusting the shaking amplitude of the shaking assembly is arranged on the shaking assembly, and a lifting assembly for adjusting the height of the incubator body is arranged at the bottom of the incubator body. And then the heating plate heats the heating plate to enable the interior of the heating plate to reach a proper temperature, then the heating plate is fixed through the fixing assembly, then the heating plate is shaken by starting the shaking assembly to prevent the heating plate from precipitating, and the shaking amplitude of the shaking assembly to the heating plate is changed by adjusting the adjusting assembly.

The invention discloses a quality detection device for mesenchymal stem cells in the technical field of cell detection, and the quality detection device comprises a bracket and two culture dishes, and the two culture dishes are symmetrically distributed on the left and right sides of the bracket; a circulation mechanism is arranged on the bracket and is used for connecting the two culture dishes so that tissue fluid in the two culture dishes can circulate mutually; butt joint mechanisms are symmetrically arranged on the left side and the right side of the bracket, and are used for butt joint of the culture dishes and the circulation mechanism in a sealing manner; according to the device, tissue fluid in a culture dish in which mesenchymal stem cell tissue fluid is cultured circulates into another empty culture dish through a circulation mechanism, each mesenchymal stem cell can be detected in real time through a detection mechanism in the circulation process, and the mesenchymal stem cell tissue fluid continuously flows through a monitoring point, so that the mesenchymal stem cell tissue fluid can be detected in real time; therefore, the influence on the culture of the mesenchymal stem cells due to long-time illumination can be effectively avoided.

The invention belongs to the technical field of biomedicine, and particularly relates to a preparation method of human nourishing stem cells induced by human extended potential stem cells, which comprises the following specific steps: step 1, converting H9 human embryonic stem cells into adult extended potential stem cells on the basis of a feeder-layer-free system: (1) coating culture dishes with Matrigel, adding 1ml of Matrigel working solution into 35 dishes, and uniformly stirring; placing in an incubator at 37 DEG C overnight, and fully coating the dish bottom with the substrate for later use; according to the application, H9 human embryonic stem cells are converted into adult extended potential stem cells on the basis of a feeder-layer-free system; based on a human trophoblast stem cell culture system, feeder-layer-free human extended potential stem cells are differentiated into induced human trophoblast stem cells, and finally the induced human trophoblast stem cells are identified and induced to differentiate, so that interference of feeder-layer cells on the induced trophoblast stem cells is eliminated, and the induction efficiency of the induced trophoblast stem cells under the feeder-layer-free system is ensured.

The invention discloses a culture device and method for tremella strain breeding, and belongs to the technical field of strain culture. A feeding cylinder is slidably connected in a sleeve, an unloading mechanism is arranged on an upper sliding plate, a lower sliding plate is arranged on a plurality of lower supporting columns, a culture bag is placed in a culture dish, and a bag opening mechanism is arranged on the lower supporting plate; a first abutting block is arranged at the bottom of the lower supporting plate in the circumferential direction, a plurality of second abutting wheels abutting against the first abutting block are rotationally installed on the lower sliding plate, and a second abutting block abutting against the discharging mechanism is arranged on the third fixing plate. In the process that a lower sliding plate drives a culture dish and a cultivation bag to move upwards, a bag opening mechanism opens an opening of the cultivation bag, the outlet end of a feeding cylinder corresponds to the opening end of the cultivation bag, a discharging mechanism on an upper sliding plate opens the opening end of a connecting cylinder, and a culture medium of the feeding cylinder enters the cultivation bag; according to the automatic bagging device, the problem that manual bagging is low in efficiency is solved, manpower is saved, and the production efficiency is improved.

The utility model discloses a citrus seedling cultivation device which comprises a base table and a rack fixedly connected to the top of the base table, a culture dish is fixedly connected to the top of the rack, a liftable placing plate is arranged in the culture dish, a first trapezoidal block is fixedly connected to the bottom of the placing plate, and a second trapezoidal block is fixedly connected to the bottom of the first trapezoidal block. The top of the base table is fixedly connected with an installation sliding rod. By arranging the liftable placing plate, citrus seedlings placed on the placing plate can be conveniently taken and placed, when the citrus seedlings are taken out, only a second trapezoidal block needs to be pulled by pulling a handle, and the inclined surface of the second trapezoidal block extrudes an extrusion roller, so that a first trapezoidal block can be upwards extruded; the placing plate can be jacked upwards, so that the placing plate can be jacked out of the culture dish conveniently, the citrus seedlings on the placing plate can be taken and placed conveniently, and the practicability of the citrus seedling cultivation device is improved.

The invention relates to a wound surface mixed bacteria unmixing method and system based on a double-branch attention mechanism, and belongs to the technical field of biomedical engineering and image processing. The method comprises the following steps: S1, acquiring hyperspectral images of a single strain culture dish, a blank culture dish and a mixed strain wound sample; s2, performing data extraction and preprocessing on the sample hyperspectral image, enhancing the data based on a spectral linear hybrid model and an adversarial generative network, and generating and synthesizing mixed bacteria hyperspectral data; s3, constructing a mixed bacteria unmixing model based on a space-spectrum double-branch attention mechanism; s4, inputting the synthesized mixed bacteria spectrum data into a mixed bacteria unmixing model for training, and optimizing parameters of the model according to the minimum total loss function of the model; and S5, inputting the hyperspectral data of the mixed bacteria wound sample to be unmixed into the trained mixed bacteria unmixing model, and outputting the unmixing abundance of the sample and the corresponding end metainformation.

The invention relates to a flame-retardant cellulose composite film and a preparation method thereof, belongs to the field of flame-retardant materials, and aims to solve the problems that a cellulose film is inflammable, poor in compatibility with an inorganic flame retardant, relatively low in mechanical property and the like. The main components of the composite membrane comprise nanocellulose, titanate nanofibers, carbon dots and a cross-linking agent. The preparation method comprises the following steps: firstly, enabling the carbon dots to react with the nanocellulose under the action of an initiator; secondly, adding titanate nanofibers into the carbon dot modified nanocellulose suspension, and uniformly mixing; then, adding a cross-linking agent and uniformly dispersing to obtain a mixed solution; and finally, casting the solution into a culture dish, and curing in a vacuum drying oven to prepare the flame-retardant cellulose composite film. According to the preparation method, the compatibility of the flame retardant and the nano cellulose is remarkably improved, the mechanical property is enhanced, and the composite film has excellent flame retardance, smoke suppression and heat insulation characteristics and is suitable for preparing the flame-retardant cellulose composite film.

The invention belongs to the field of functional material preparation, and particularly relates to a preparation method of a silicon-yttrium-carbon composite lithium ion battery negative electrode material, which comprises the following steps: (1) dispersing silicon nanoparticles, polyvinylpyrrolidone and yttrium salt in a deionized water solution to form a mixed solution, carrying out ultrasonic vibration dispersion, and then carrying out magnetic stirring in a room temperature air atmosphere; (2) transferring the obtained solution to a glass culture dish, and performing freeze drying in a freeze dryer; (3) transferring the precursor obtained by drying into a crucible, and placing the crucible in a tubular furnace for high-temperature calcination to obtain a silicon-yttrium-carbon composite material; and (4) grinding and mixing the obtained product with sodium alginate, conductive carbon black and deionized water respectively, uniformly coating the surface of a copper foil with the mixture, and then performing drying, slicing and tabletting treatment to obtain a target product. The preparation method is simple in synthesis process, good in electrode cycling stability, stable in structure and excellent in electrochemical performance.

The utility model relates to the technical field of bone marrow cell preparation, in particular to a bone marrow cell preparation device which comprises a bottom plate, stand columns are arranged on the two sides of the bottom plate, a culture dish is arranged on the bottom plate between the two stand columns, and rotating rods are rotationally arranged on the opposite sides of the two stand columns at the same height. The ends, away from the corresponding stand columns, of the two rotating rods are close to each other, the two rotating rods are horizontally arranged, vertical plates are arranged at the ends, close to each other, of the two rotating rods, springs are arranged on the sides, away from the corresponding rotating rods, of the vertical plates, clamping plates are arranged at the ends, away from the corresponding vertical plates, of the springs, and the two clamping plates are used for clamping bones. One rotating rod penetrates through the corresponding stand column and then is connected with a mounting plate, an abutting bolt is connected to the mounting plate, the abutting bolt penetrates through the mounting plate and then abuts against the side wall of the corresponding stand column, a top plate is arranged at the tops of the two stand columns, a flushing fluid box is arranged on the top plate and communicates with a flushing pump, and the pump-out end of the flushing pump communicates with a needle head. The bone marrow cells can be prepared more conveniently.

The invention provides a top-down directional evolution method for growth-promoting microbiome of rice. The top-to-bottom rice growth-promoting microbiome directional evolution method comprises the following steps: step 1, selecting a microbiome with a growth-promoting effect on rice seedlings, combining Bottleneck + Migration into an LB culture medium for iterative culture, and after 15 rounds of non-selective iteration, transferring each round of iteration into a new LB culture solution; 2, respectively inoculating the flora subjected to 15 rounds of non-selective iteration in the step 1 into a Hoagland nutrient solution; 3, the nutrient solution samples with the flora added in the step 2 are respectively and equivalently added into rice seedling culture dish samples; 4, observing the growth vigor of the rice once every five days, and detecting morphological indexes such as leaf length and root length of the rice; 5, repeating the steps 1-4 on the inoculated flora screened in the step 4, and circulating for 5 times; the method has the advantage of screening out the microbiome capable of remarkably promoting the growth of the rice seedlings.

The utility model discloses a microbiological incubator for medical research in the technical field of microbiological culture equipment, which comprises an incubator body, universal wheels are mounted at the bottom of the incubator body, a partition plate is fixedly mounted in the incubator body, and rotating rods are rotatably connected to the top of the partition plate and the bottom of an inner cavity of the incubator body. Placing tables are connected to the top ends of the multiple sets of rotating rods, multiple sets of placing grooves which are circumferentially and equidistantly arranged around the central axis of the placing tables are formed in the tops of the placing tables, positioning assemblies for fixing the placing tables are installed at the tops of the partition plates and the bottom of an inner cavity of the box body, and circular containing grooves are formed in the centers of the tops of the placing tables. The microbial incubator solves the problems that when an existing microbial incubator is used, a certain number of culture dishes are often placed in the microbial incubator, the culture dishes in the deep position of the incubator body are inconvenient to pick up, and people cannot use the microbial incubator conveniently.

The invention provides a preparation method and application of a mixed matrix membrane based on in-situ MOF gel, and relates to the field of gas separation membrane materials. The preparation method comprises the following steps: weighing a certain amount of dry Pebax particles, and dissolving the Pebax particles in an ethanol-water mixed solvent at 90 DEG C to obtain a solution A; dissolving zinc nitrate hexahydrate in an ethanol-water mixed solvent to obtain a solution B; dimethylimidazole is dissolved in an ethanol-water mixed solvent, and a solution C is obtained; adding a certain amount of triethylamine into the solution C, and uniformly mixing; sequentially adding the solution B and the solution C into the solution A, and stirring for reaction to obtain a mixed solution; transferring the mixed solution to a dialysis bag for dialysis; and transferring the dialyzed mixed solution to a polytetrafluoroethylene culture dish, and drying to obtain the mixed matrix membrane. The preparation method provided by the invention is simple and easy to operate, the prepared mixed matrix membrane has good interfacial compatibility with MOF gel, and the MOF loading capacity can be improved, so that the gas separation performance is improved.

The invention relates to the technical field of biology, and particularly discloses a device and a method for separating an adherent cell through a vortex sound field, an ultrasonic module, piezoelectric ceramics and an Archimedes spiral structure concave lens are arranged to generate the vortex sound field to act on a cell culture dish, so that an adjustable shear flow is generated on the cell culture dish, and the adherent cell is separated from the cell culture dish. And locally separating the adherent cells in the cell culture dish. According to the concave lens with the Archimedes spiral structure, the problem of defocusing of a high-order vortex sound field can be solved, and the falling control accuracy of adherent cells is improved. The proposed method does not require any physical contact with adherent cells, and does not require insertion of an instrument into a cell culture dish, thereby reducing the risk of contamination and cell damage. The stable flow generated by absorbing sound energy by the liquid is used for indirectly separating the adherent cells, the method is extremely low in energy density and has good biocompatibility, and the activity of the cells is ensured while the cells are efficiently separated.

The invention discloses a cell culture box convenient for taking and placing cell culture dishes, and relates to the technical field of cell culture, the cell culture box comprises a box body, the inner wall of the box body is fixedly connected with a plurality of placing plates, and the side walls of the top ends of the plurality of placing plates are provided with a plurality of first grooves. The inclination angle of the second clamping plate is the same as that of the outer wall of the corresponding cover plate, so that the cover plate is screwed above the cell culture dish by utilizing the second clamping plate, the primary installation between the cell culture dish and the cover plate is completed, and then a buckle on the outer side of the cover plate can be pressed into a clamping groove in the outer wall of the cell culture dish through a clamping block; when the cell culture dish needs to be taken out, a buckle is pulled out of a clamping groove in the outer wall of the cell culture dish by utilizing a pulling plate, and then the cover plate is taken down from the cell culture dish by utilizing a second clamping plate, so that the culture efficiency and reliability during cell culture are greatly improved.

The invention discloses a cell detection device, and relates to the technical field of cell detection. The cell detection device comprises a transmission part arranged in a transmission frame, and the transmission part is assembled to enable two arc-shaped clamping plates to move towards each other or away from each other after moving upwards; and after the downward movement, the placing disc can vibrate. According to the device, the placement disc, the supporting frame, the transmission frame and the transmission part are arranged, after a culture dish is placed on the placement disc, the transmission part which moves upwards can enable arc-shaped clamping plates on the transmission part to move oppositely, so that the culture dish is fixed, and after the transmission part moves downwards, the transmission frame can be driven to vibrate through vibration; when a coloring agent is injected, the placing disc can drive the culture dishes to rotate, uniform dyeing is realized, the problem that the culture dishes with different sizes are inconvenient to fix in the prior art is solved, and meanwhile, the dyed culture dishes are vibrated, so that rapid fusion dyeing of the coloring agent and cells is realized; the dyeing period is long.

The invention discloses a preparation method of a metal organic framework gel mixed matrix membrane and application of the metal organic framework gel mixed matrix membrane in CO2 / CH4 separation, and belongs to the technical field of gas separation membranes. The method comprises the following steps: dispersing metal organic framework (MOF) gel into a solvent to obtain a solution containing an MOF gel network; then, sequentially adding diamine and dianhydride monomers into the solution, and carrying out polymerization reaction to directly prepare a mixed matrix membrane casting solution; then, uniformly coating a glass culture dish with the membrane casting solution, and finally obtaining the mixed matrix membrane through the steps of heat treatment, cleaning and drying. The method is mild in preparation condition and simple to operate. The MOF gel is selected as a filler, a continuous transmission channel is constructed in a solvent, and in-situ polymerization is performed to form a polyimide matrix, so that the permeation flux of the separation membrane is remarkably improved, and the interfacial compatibility between the filler and the matrix is effectively enhanced. The prepared mixed matrix membrane shows excellent CO2 / CH4 separation performance.