1172 results about "Biological safety" patented technology

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

The invention provides a metal polyphenol network coated self-assembled nano-drug as well as a preparation method and application thereof, and belongs to the technical field of nano-drugs. The self-assembled nano-drug provided by the invention is of an organic-inorganic hybrid core-shell nano-structure; the core of the organic-inorganic hybrid core-shell nano structure is BC NPs; the BC NPs is formed by self-assembly of BBR (Boron Butadiene Rubber) and Cur (Carbon); a shell layer of the organic-inorganic hybrid core-shell nanostructure is a dynamic cross-linked network formed by self-assembly of metal ions and polyphenol through coordination. Metal ions and polyphenol are self-assembled through coordination to form a dynamic cross-linked network, and the dynamic cross-linked network coats the outer layer of the BC NPs nano-particles, so that the BC NPs nano-particles have good biocompatibility and biological safety; and the self-assembled nano-drug shows an obviously enhanced antibacterial effect under the combined treatment of NIR and H2O2.

The invention belongs to the cross technical field of nanomedicine, tumor treatment and immunotherapy, and particularly provides tumor mitochondria-targeted copper death selective induction nanoparticles, which are prepared by the following preparation method: S1, specifically coupling TPY and MHI through condensation reaction to obtain MTPY; s2, MTPY and copper ions are subjected to complexation, and an MTPY-Cu complex is obtained; and S3, the MTPY-Cu complex and albumin are subjected to self-assembly, and the MTPY-Cu-coated Alb nanoparticles are formed. The dosage of copper is only 1 / 1000 of that of free Cu < 2 + >, and equivalent immune regulation and killing effects can be achieved; primary tumors and distant tumor focuses can be inhibited, and lung metastasis is reduced; immune memory can be induced, and relapse can be prevented; the traditional Chinese medicine composition is combined with cis-platinum to remarkably enhance the curative effect and reverse the induced immunosuppression; the invention has the advantages of high biological safety and no obvious liver and kidney toxicity or hemolytic reaction.

The invention discloses a preparation method of a bionic cornea stroma lens for refractive correction, which comprises the following steps: solution preparation: dissolving type I collagen in an acetic acid solution, adding a film aid, fully stirring, and centrifugally defoaming to obtain a type I collagen composite solution; the I-type collagen composite solution is poured into an assembling groove, the curvature radius of titanium metal is set to be 6.8-8.0 mm, the titanium metal serves as a working electrode material, platinum metal serves as a counter electrode material, current parameters are set according to the needed thickness of the lens blank, the density is 3.50-6.50 mA / cm < 2 >, and the thickness of the lens blank is set to be 3.50-6.50 mA / cm < 2 >; a high-transparency cornea embryo material which is uniform in curvature in all directions and similar to human cornea fiber structure arrangement is formed after electrochemical assembly and cross-linking curing, accurate cutting machining is carried out through excimer laser, a concave / convex matrix lens with the needed correction degree is obtained, the correction precision is high after cornea interlayer implantation, and the correction precision is high. And meanwhile, the compound has excellent performance in the aspects of in-vivo stability and biological safety.

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention discloses an eel antibacterial polypeptide Ajaspkidin and application thereof in preparation of an anti-vibrio composition. The amino acid sequence of the eel antibacterial polypeptide Ajaspkidin is shown as SEQ ID NO.01. The invention further discloses an application of the eel antibacterial polypeptide The zebra fish antibacterial peptide has the characteristics of excellent molecular characteristics, broad-spectrum antibacterial activity, efficient bactericidal ability, high thermal stability, high biological safety, anti-infection effect on zebra fish infected by vibrio vulnificus, capability of remarkably improving the survival rate of bred animals and the like, and has a wide application prospect.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention discloses a bacteriostatic and antiviral bamboo pulp paper-based sanitary isolation bag as well as a preparation method and application thereof, and belongs to the field of functional paper-based packaging materials. The isolation bag takes bamboo pulp fiber paper as a base material, and the surface of the isolation bag is coated with a water-based polyhydroxyalkanoate functional coating. According to the preparation method, a special polyphenol derivative-chitosan quaternary ammonium salt-nano zinc oxide organic-inorganic hybrid factor is introduced, and the problem of agglomeration of nano particles is effectively solved by utilizing the special bridging effect of polyphenol, so that high-density cation capture and oxidation inactivation sites are constructed on the surface of the coating. Compared with the prior art, the method has the advantages that the method does not need to depend on organic fluorine compounds, and the excellent waterproof and oil-proof barrier effect and the efficient inactivation of viruses can be achieved only through the extremely low coating amount. In addition, the isolation bag has unique environmental chemical responsiveness, can be efficiently dissociated under the alkaline condition to facilitate repulping recovery, can be completely biodegraded in the natural environment, and perfectly considers the requirements of biological safety protection and green environmental protection.

The invention relates to an anti-inflammatory and repair-promoting hydrogel for a uterine cavity as well as a preparation method and application of the anti-inflammatory and repair-promoting hydrogel, and relates to the technical field of biological medicines. The uterine cavity anti-inflammatory and repair-promoting hydrogel is prepared from the following raw materials: an anti-inflammatory component, a biomacromolecule with active ester and an amino high-molecular polymer. The hydrogel for resisting inflammation and promoting repair of the uterine cavity, which is prepared by the invention, has good biological safety, injectability and adhesiveness with tissues; in intrauterine adhesion prevention treatment, a three-dimensional network structure formed by crosslinking of the biomacromolecules with the active ester and the amino high-molecular polymer plays a physical isolation role, the anti-inflammatory component plays an anti-inflammatory role by inhibiting expression of an inflammation signal channel, and the biomacromolecules with the active ester and the amino high-molecular polymer achieve a synergistic treatment effect.

The present application relates to the technical field of aquaculture monitoring and regulation, and discloses a water quality multi-parameter real-time online monitoring system. The system comprises a multi-source data acquisition module for obtaining and denoising water quality data; a time sequence feature analysis module for generating multi-dimensional feature clusters containing the correlation of various parameters; an anomaly identification module for dynamic deviation compensation, monitoring sub-domain demarcation and key parameter extraction; a collaborative regulation module for constructing a regulation decision unit to generate a multi-parameter collaborative regulation strategy; and an iterative correction module for correcting the regulation strategy to output real-time adjustment instructions. An early warning module is also provided for biological safety assessment and early warning and activation of the emergency intervention mechanism. The system can accurately monitor the multi-parameters of the water quality of aquaculture in real time, realize intelligent regulation and biological safety early warning, effectively improve the yield and quality of aquaculture, reduce the risk of aquaculture, and promote the sustainable development of the industry.

The invention discloses an oligonucleotide intracellular nano delivery system based on polypeptide modification and application thereof. The system is composed of a periostin targeting sequence (SDSSD), a matrix metalloproteinase 2 (MMP2) response sequence (GPAGLLG), a cell penetrating sequence (RRRRRRRR, R9), a reactive oxygen species (ROS) scavenging and adhesion enhancing group (Gly-DOPA)) and a terminal dibenzocyclooctyne (DBCO) modified engineered polypeptide SDSSD-PEG5-YGFGG-GPAGLLG-R9-(G-DOPA) 3-K4-C-DBCO, and a target oligonucleotide miRNA-26a-A5-Azido modified by 5-polyadenylic acid (AAAAA) and an azide group (Azido), and the target oligonucleotide miRNA-26a-A5-Azido, the target oligonucleotide and assembling through a click chemical reaction and a non-covalent interaction. The nano system has good bone targeting, enzyme responsiveness, intracellular delivery effect and biological safety, can realize stable and efficient delivery of therapeutic oligonucleotides in vivo, and significantly improves the utilization efficiency and therapeutic potential of oligonucleotides. The oligonucleotide intracellular nano delivery system has a wide application prospect in the fields of clinical transformation and precise treatment of oligonucleotide drugs.

The invention provides an exosome from rice as well as a preparation method and application thereof. The exosome has a double-layer membrane spherical structure, and the particle size range is 20-1000nm. The exosome is obtained from rice calluses, and has the characteristics of high biological safety, high stability and sustainable mass production. And tests prove that the exosome has the functions of resisting aging, promoting cell regeneration and tissue repair, protecting and the like, and also can remarkably improve the cell absorption efficiency and the delivery efficiency of active ingredients such as peptide cells.

The invention relates to the technical field of data processing, and discloses a port special article quick retrieval method and system based on data processing, and aims at complexity of modal features of special articles by collecting characterization information such as images and characters of the special articles. An image and object information unified characterization technology, a similarity measurement method and a feature association technology are applied, a retrieval model for biosecurity risk monitoring is established by utilizing an industry knowledge graph, non-trade individual carrying and delivery channel biosecurity target feature matching is realized, multi-language special article high-risk factors are identified, and a high-risk result is obtained. The accuracy of biological safety supervision risk study and judgment of special articles carried by non-trade individuals and posting and delivering channels and the working efficiency and the working quality of tour inspection channels and posting and delivering channel business sites are improved; the supervision situation that existing biological safety risk monitoring of special objects carried by non-trade individuals and special objects carried by delivery channels basically depends on personal experience and ability of experts to judge and make decisions is optimized.

The invention relates to the technical field of sewage treatment and gas monitoring control, in particular to a distributed monitoring system for gas emission tracking. The system comprises a working condition data acquisition unit, an emission reference modeling unit, a model deviation analysis unit, a vulnerability deduction unit and an anti-vulnerability control unit. The system obtains a predicted emission value by constructing a physical information neural network model, compares the predicted emission value with a measured value to calculate a deviation, and generates an attack state flag; the method is characterized in that a microbial flora vulnerability index is calculated in combination with an attack state flag and a carbon source fluctuation rate; when the vulnerability index exceeds a preset biological safety threshold value, the system adaptively switches to a biological priority mode; according to the method, real-time quantification of the credibility of the model is realized, and invisible biochemical attacks with unknown toxicity sources can be effectively identified.

The invention relates to the technical field of aquaculture monitoring, regulation and control, and discloses an aquatic product water quality multi-parameter real-time online monitoring system. The system comprises a multi-source data acquisition module for acquiring and denoising water quality data; the time sequence feature analysis module is used for generating a multi-dimensional feature cluster group containing a plurality of parameter association relationships; the abnormity identification module is used for carrying out dynamic deviation compensation, delimiting a monitoring sub-domain and extracting key parameters; the coordinated regulation and control module constructs a regulation and control decision unit to generate a multi-parameter coordinated regulation and control strategy; and the iterative correction module is used for correcting the regulation and control strategy and outputting a real-time regulation instruction. And an early warning module is also arranged to carry out biological safety evaluation and early warning and activate an emergency intervention mechanism. The system can accurately monitor multiple parameters of aquaculture water quality in real time, realizes intelligent regulation and control and biological safety early warning, effectively improves the yield and quality of aquaculture, reduces the aquaculture risk, and promotes the sustainable development of the industry.

The invention discloses a microbial fertilizer with growth-promoting and yield-increasing effects and a preparation method thereof. The microbial fertilizer is prepared by mixing a hydrogel fertilizer and a microbial agent, the hydrogel fertilizer is formed by taking chitosan as a base material and carrying out cross-linking reaction with 2, 2-dimethyl-5-hexenoic acid, N-(N-butoxymethyl) acrylamide and urea, and the microbial agent is prepared by loading bacteria N9 separated from farmland soil on biochar. The microbial fertilizer has good water retention performance and nutrient slow release effect, the contained microorganisms can effectively promote growth, tests show that the soil water retention rate can be remarkably increased, growth of pakchoi and other crops is promoted, the biological safety is high, and the microbial fertilizer is suitable for achieving crop growth promotion and yield increase in agricultural production.

The invention discloses design and synthesis of a series of brain-targeted ginsenoside Rg1 derivatives and application of the brain-targeted ginsenoside Rg1 derivatives in treatment of Alzheimer's disease. According to the invention, ginsenoside Rg1 and polyethylene glycol with a specific chain length are covalently linked to successfully construct the derivative capable of enhancing the penetrating power of the blood-brain barrier. The series of derivatives provided by the invention not only solve the problems of poor brain targeting and insufficient stability of natural Rg1, but also can inhibit neuroinflammation by regulating and controlling an NLRP3 / caspase-1 signal channel in an LPS-induced neuroinflammation model, and finally, the learning and memory ability is remarkably improved. The product is simple and convenient in preparation process and good in biological safety, and a new candidate compound is provided for developing a new generation of Alzheimer disease treatment medicines.

The invention relates to the field of microbiological detection, in particular to a method for detecting vibrio parahaemolyticus by combining RPA-CRISPR / Cas12a with a microfluidic biosensor. According to the method, the preloaded freeze-drying reagent is combined with centrifugally driven liquid operation, so that rapid and closed system detection is realized, and the pollution risk is effectively reduced. According to the invention, quantitative detection of vibrio parahaemolyticus is realized in a centrifugal micro-fluidic chip by systematically optimizing an RPA amplification primer and crRNA. The detection method shows high specificity and high sensitivity to non-target vibrio and other food-borne bacteria, and the lower detection limit is 6.08 copies / mu L. In a word, the invention provides a rapid, portable and sensitive platform, can realize quantitative detection in a wide linear range, provides a practical tool for real-time monitoring of pathogens, and has application prospects in monitoring of aquaculture biosafety and marine product safety.

The invention belongs to the technical field of drug delivery, and particularly relates to an M-coated PLGA-10BX compound as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing 10B-enriched boron nitride (h-10BN); extracting a cell membrane of the macrophage RAW264.7; polylactic acid-glycolic acid copolymer (PLGA) nano particles loaded with h-10BN are prepared; and finally, the bionic nano platform M (at) PLGA-10BN based on the macrophage membrane is prepared. The bionic nano-platform prepared by the invention is uniform in particle size and morphology, has relatively high biological safety, and has no obvious damage to various tissues and visceral organs. The compound can be used as a general platform for boron compound delivery, can also be used as an immune activator, is suitable for intravenous injection administration, and expands the application of boron neutron capture therapy (BNCT) in treatment of glioblastoma (GBM).

The invention provides an alginate fiber sanitary towel and a preparation method thereof, and belongs to the technical field of nursing materials. Through the directional water-repellent design of the double-sided reverse osmosis prevention functional layer and in combination with the three-dimensional leakage-proof partition edge structure, the risks of liquid back seepage and side leakage are remarkably reduced, and the use dryness and reliability are improved; the natural antibacterial effect of the alginate fibers is utilized, efficient peculiar smell control is achieved, irritation of traditional chemical bacteriostatic agents to the skin is avoided, and biological safety is guaranteed; the core material adopts alginate fiber renewable resources, the characteristics of high water absorption and degradability of the alginate fiber absorber are combined, the environmental protection sustainability of the product is enhanced, the common problems of reverse osmosis, side leakage, dependence on chemical addition and the like of the sanitary towel are solved, meanwhile, the unification of the protection performance, the health attribute and the sustainability is achieved through material and structure innovation, and the sanitary towel is suitable for being used for sanitary napkins. And a technical normal form capable of being flexibly expanded is provided for the industry.

The invention discloses a nucleic acid medicinal preparation based on dialkyl imidazole cationic lipid as well as a preparation method and application thereof, and belongs to the field of medicinal preparations. The nucleic acid pharmaceutical preparation comprises drug-loaded lipid nanoparticles, and the drug-loaded lipid nanoparticles comprise one or more dihydrocarbyl imidazole cationic lipids. By adjusting lipid composition and proportion in the drug-loaded lipid nanoparticles, efficient loading and delivery of different types of nucleic acid drugs can be realized; the drug-loaded lipid nanoparticles have good biological safety, the preparation process is simple, and large-scale production is easy; the drug-loaded lipid nanoparticle can effectively pass through a blood brain barrier, realizes specific brain-targeted delivery of the loaded nucleic acid drug, and provides a new strategy for realizing brain-targeted delivery of different nucleic acid drugs for treating brain diseases.

A system, method, and receiver control architecture for a Symbolic Resonant Energy Transfer Network (SRETN) configured to distribute electrical power wirelessly across planetary-scale distances under cryptographic, economic, and biological safety governance. The system comprises a distributed mesh of terrestrial resonant transmission towers and orbital relay satellites configured to deliver energy via directed magnetic resonance coupling. A Symbolic Energy Kernel governs authorization and transmission, preventing energization of any transmission vector unless a secure cryptographic energy handshake is completed. The handshake verifies: (i) authenticity and compliance of a requesting receiver device; (ii) real-time solvency of an associated decentralized energy ledger account; and (iii) clearance of the transmission path through active radar and lidar-based biological exclusion scanning. Energy is transmitted as discrete, tokenized packets, each cryptographically metered and acknowledged by the receiver prior to continuation. The system enables a wireless, infrastructure-light energy distribution paradigm in which electrical power is accessed on demand with transaction-level auditability and enforced safety constraints.

The present invention relates to a dental diaphragm copolyester and a preparation method thereof, the preparation method comprises the following steps: 2, 5-furandicarboxylic acid or an ester thereof, aliphatic or alicyclic diol and (2-hydroxy-5-formyl phenyl) diphenylborane are subjected to a reaction to obtain an esterification or ester exchange product; and carrying out condensation polymerization on the esterification or ester exchange product and the stabilizer to prepare the copolyester for the dental diaphragm. 2, 5-furandicarboxylic acid is derived from a renewable biomass resource and meets the requirements of green and sustainable development; compared with terephthalic acid and furan ring structures, the film has higher rigidity and polarity, the yield of hot press molding is improved, the same correction force can be met by a thinner film, the wearing comfort of a patient is improved, and meanwhile stress relaxation is improved; the catalyst perfectly combines strong Lewis acidity, intramolecular stabilization and potential bifunctional activation through structural design, provides possibility for efficient and green synthesis of polyester, avoids use of heavy metal, and is higher in biological safety.

The invention belongs to the technical field of biological materials, and particularly relates to polypeptide MSE for enzyme-responsive targeted degradation of EGFR protein and anti-tumor application of the polypeptide MSE. The amino acid sequence of the polypeptide MSE is as shown in SEQ ID NO. 1. The polypeptide MSE can be combined with EGFR protein on the surface of a cell membrane in a co-localization mode, degrade the EGFR protein on the cell membrane and promote tumor cell apoptosis through an active oxygen signal channel, has an anti-tumor effect in vivo and in vitro, has good biological safety and is expected to provide a new thought for cancer treatment.

The invention relates to the technical field of medical cosmetology, in particular to a porous hollow glass bead capable of slowly releasing collagen and application of the porous hollow glass bead. The porous hollow glass bead capable of slowly releasing the collagen comprises a porous hollow glass bead body, the outer wall of the porous hollow glass bead body is provided with a pore channel structure penetrating through the exterior and an inner cavity of the hollow glass bead body, and the pore channel and the inner cavity of the porous hollow glass bead body are filled with collagen gel. The compressive strength of the hollow glass beads is utilized to improve the mechanical supporting effect, the collagen gel is subjected to catalytic decomposition under the action of collagenase in a body so as to achieve the slow-release effect, the outer walls of the hollow glass beads slow down the reaction time through physical isolation, and then the slow-release time is prolonged; meanwhile, ions such as Si and Ca released by the hollow glass beads can promote cells to secrete collagen, the biological activity is improved, and the three beneficial effects of physical support, collagen slow release and biological safety are achieved.

The invention relates to the technical field of vaccines, and discloses a preparation method and application of an anti-tumor vaccine based on an autoantigen and a synthetic antigen. The anti-tumor vaccine comprises a tumor autoantigen and a synthetic antigen, wherein the tumor autoantigen is extracted from a tumor cell membrane, a tumor cell lysate, a tumor cell secretion, an exosome antigen or an antigen released by a tumor microenvironment; the synthetic antigen is selected from at least one of a tumor associated antigen, a tumor specific antigen, a personalized neoantigen, a pathogen antigen, a biotoxin, a biomolecule antigen and coding nucleic acid of a peptide type antigen in the antigens; the coding nucleic acid is DNA or mRNA. The vaccine molecule composed of the tumor autoantigen and the synthetic antigen is easy to prepare, has a strong anti-tumor immune effect and good biological safety, solves the problems that the traditional tumor autoantigen has no specific precise target spot and the synthetic antigen is single in immunogen, and has a wide application prospect.

The invention discloses a multi-target joint optimized antibiotic molecule design system and method applied to the field of biological medicine, and the method comprises the steps: obtaining FabI enzyme and AcrAB-TolC efflux pump structure data, extracting double-target space and physicochemical characteristics, and constructing a joint pharmacophore model containing a FabI inhibition domain and an efflux pump blocking domain; screening candidate molecules meeting a double-domain space matching threshold, screening high-affinity double-target molecules through conformation sampling and free energy calculation, and forming a lead compound library through multi-layer filtration of metabolic stability, membrane penetrability, quantum chemical properties and synthesis feasibility; carrying out electron effect, chain length or heteroatom fine tuning on dominant molecules through in-vitro bacteriostasis and efflux inhibition experiment feedback, and carrying out iterative optimization until MIClt is met; 2 [mu] g / mL and the efflux inhibition rate is gt; according to the present invention, the antibacterial effect and the drug resistance inhibition ability are significantly improved, the molecular synthesizability and the biological safety are ensured, and the engineering design new path is provided for the Gram-negative bacterium drug resistance crisis.

The gadolinium-based cationic polymer contrast agent is synthesized by chelating a cationic polymer and gadolinium ions, has high biological safety and can be used for cartilage imaging. The prepared contrast agent adopts an articular cavity direct injection method, so that the imaging effect is improved, and the contrast dosage is reduced. When the cartilage activity is normal, the content of glycosaminoglycan in the matrix is rich, and when the cartilage is inactivated or the activity is reduced, the content of glycosaminoglycan in the matrix is obviously reduced. The gadolinium-based cationic polymer contrast agent is combined with negatively charged glycosaminoglycan in a cartilage matrix through the charge effect. The gadolinium-based cationic polymer contrast agent can be used for noninvasive and quantitative evaluation of cartilage activity in nuclear magnetic resonance imaging, especially cartilage activity and stability in transplantation.

The invention belongs to the technical field of medical wound materials, and relates to conductive nanofiber aerogel for preventing and treating stress injury and a preparation method thereof. The conductive nanofiber aerogel comprises nanofiber aerogel, a polypyrrole conductive network and a polydopamine hydrophilic layer, wherein the polypyrrole conductive network and the polydopamine hydrophilic layer are deposited in the nanofiber aerogel; the nanofiber aerogel is composed of nanofibers containing a first methylacryloylated polymer and polylactic acid and a second methylacryloylated polymer, the nanofibers are in staggered lap joint to form a three-dimensional skeleton, and the lap joint is wrapped by the second methylacryloylated polymer. The preparation method comprises the following steps: preparing nanofiber aerogel, and sequentially infiltrating the nanofiber aerogel into a pyrrole / FeCl3. 6H2O solution and a dopamine hydrochloride / Tris-HCl solution to react. The conductive nanofiber aerogel has good structural stability, biological safety, hydrophilic imbibition and pressure / seepage sensing capability, and can play an efficient role in prevention and treatment of pressure injury.

The invention discloses an ultrasonic controllable multifunctional microneedle patch as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The preparation method comprises the following steps: preparing crystalline CoW-LDH nanosheets from Co (NO3) 2.6 H2O, Na2WO4. 2H2O and NaOH, dispersing the crystalline CoW-LDH nanosheets in PBS (Phosphate Buffer Solution), centrifuging and collecting the crystalline CoW-LDH nanosheets, and re-dispersing the crystalline CoW-LDH nanosheets in ultrapure water to obtain amorphous CoW-LDH nanosheets; adding PEG (Polyethylene Glycol), ultrasonically dispersing, stirring at room temperature, centrifuging, collecting and centrifugally washing with ultrapure water to obtain a PEG modified amorphous CoW-LDH nanosheet; and adding microalgae and an amorphous CoW-LDH nanosheet modified by PEG (Polyethylene Glycol) to prepare the ultrasonic controllable multifunctional microneedle patch. The multifunctional microneedle patch disclosed by the invention is high in biological safety, can realize ultrasonic controllability, has a sonodynamic treatment effect and an oxygen supply effect, remarkably inhibits melanoma recurrence and remarkably promotes wound healing, and has a wide application prospect.