1770 results about "Biological safety" patented technology

The invention discloses a novel coronavirus pneumonia (COVID-19) serological diagnosis kit. The kit comprises an S-IgM / IgG test strip and an N-IgM / IgG test strip, the double-antigen quadruple detection kit can be used for simultaneously detecting four indexes of an IgM / IgG antibody for resisting novel coronavirus spinous process protein S and an IgM / IgG antibody for resisting novel coronavirus nucleocapsid protein N in serum of a patient suffering from novel coronavirus pneumonia COVID-19. According to the kit, the detection sensitivity is improved through quantum dot fluorescence labeling andmultistage coupling amplification signals, the detection accuracy is improved through double-antigen quadruple detection, and the biological safety in the detection process is guaranteed by establishing a virus inactivation system. The kit is suitable for whole blood, plasma and serum detection, and can be applied to novel COVID-19 serological diagnosis.

The invention discloses an in-situ composite system based on a carbon quantum dot / manganese dioxide nanometer sheet layer and a using method of the in-situ composite system. The using method comprises the following steps: firstly, completely quenching fluorescence by using a manner that carbon quantum dots are adsorbed on a manganese dioxide nanometer sheet layer prepared by reducing potassium permanganate in situ, and then quantitatively analyzing and determining the content of the GSH in a GSH to-be-tested sample based on a principle of causing digestion of the manganese dioxide nanometer sheet layer, releasing the carbon quantum dots and recovering the fluorescence thereof by virtue of a redox reaction between glutathione (GSH) and manganese dioxide. According to the in-situ composite system, the detection method is high in precision, the repeatability is good, a used carbon material is low in price, easily available, and free from polluting the environment, and has relatively good biological safety; meanwhile, the in-situ composite system is simple and convenient to operate, relatively strong in practicability, high in sensitivity, and excellent in selectivity of the GSH.

The invention provides a cellulose / two-dimensional layered material composite hydrogel. The composite hydrogel comprises a cellulose three-dimensional network structure and a two-dimensional materialsupported in the cellulose three-dimensional network structure, wherein the two-dimensional layered material is a two-dimensional transition metal carbide, nitride, or carbonitride. According to the composite hydrogel provided by the invention, the two-dimensional layered material can be stably supported in the composite hydrogel system and is not easy to agglomerate; and the composite hydrogel has good compatibility with a biological fluid, and the advantages of full biodegradability and high biological safety, and can be used in the field of biomedicines. The invention also provides a preparation method of the composite hydrogel.

A movable laboratory with high biological safety is composed of primary experiment cabin, person cleaning and technological supporting cabin including clothes exchanging room, buffering room and substance transfer window between them, air-tight flexible connection channel between said both cabins, and the connected water and air pipelines, cables and signal lines.

The invention relates to a preparation method of a biological mimetic and structural bionic tissue adhesive in order to overcome disadvantages of present technological technologies for preparing mussel mucoprotein bionic biological tissue adhesives and improve the viscosity, the biocompatibility and the structural bionic property of a material. The preparation method comprises the following steps: grafting a natural polymer material with dopamine or other catechol group-containing derivatives through an amidation reaction by adopting DMTMM as a carboxyl group activator; and further modifying the grafted natural polymer material with lysine or polylysine by adopting the same technology to obtain the novel biological mimetic and structural bionic tissue adhesive containing dopamine analog and polylysine analog. The tissue adhesive plays a full role in tissue adhesion in wet and physiologic environment, has good tissue adherence and high biological safety, and can be slowly degraded in the tissue healing process until complete degradation. The preparation method of the biological adhesive has the advantages of simplicity, mild reaction conditions, increase of the reaction efficiency, improvement of the operability of the enterprise production process, and facilitation of amplified production of enterprises.

The present invention discloses a construction of three-gene defected recombinant pseudorabies virus (PrV) strain, vaccine prepared by said strain, method for constructing said strain and method for preparing said vaccine. The described recombinant pseudorabies virus strain defects genes of TK.gE ang gI, and contains no exogenous gene. The described vacine belongs to the freeze-dried live vaccine made up by virus liquid containing said invention and gelatin. Said invented live vaccine can be inoculated on the piglet, slaughter pig and sow with farrow, and can obtain obious immune effect. Said vaccine has good biological safety, and can be used for preventing and curing pseudorabies.

The invention discloses bacillus pumilus, a probiotics preparation and a preparation method and application thereof. The Bacillus pumilus LV149 is preserved in China center for type culture collection (CCTCC) in Nov. 23th, 2011, and the preservation number is CCTCC NO: M 2011411. The bacillus pumilus LV149 has strong extracellular protease, lipase and amylase activities, has wide rejection capability to vibrio and has no hemolytic activity. The Bacillus pumilus LV149 serves as fermenting bacterial strains and is performed with solid fermentation, drying and smashing so as to prepare bacillus pumilus probiotics preparation which uses Bacillus pumilus LV149 as the active ingredients. The bacillus pumilus probiotics preparation can be added into prawn feeds for feeding prawns, so that growth of prawn intestinal pathogenic vibrio can be restrained, prawn vibriosis can be reduced, simultaneously prawn growth is promoted, fish bait coefficient is reduced, quality of commodity is improved, culture cycle is shortened, accordingly culture risk and culture cost are reduced, biological safety is high and the bacillus pumilus, the probiotics preparation and the preparation method and application thereof have wide application prospect in aquaculture.

Belonging to the technical fields of biology and food, the invention relates to a preparation method of an active lactobacillus pickle pickling liquor. The method includes: taking a fermentation pickling solution of pickled pepper, a fermentation pickling solution of pickled capsicum frutescens and cold boiled water as raw material water, subjecting the pickling solutions to rough filtration, and mixing them with the cold boiled water uniformly, then carrying out cooling and high speed centrifugation degerming, and adding a direct vat set lactobacillus starter, thus obtaining the pickling liquor. The method provided in the invention realizes standardized preparation of pickling liquor, ensures the viable count of lactobacilli in the pickling liquor, endows the newly prepared pickling liquor with the flavor of aged pickling liquor and better flavor. At the same time, the use problem of the pickling solutions of pepper and capsicum frutescens is solved, the environmental pollution caused by emission and the potential safety hazard caused by repeated use in the next year can be avoided, so that the biological safety, quality and homogeneity of the product are significantly improved. Further, upgrading and industrial, large-scale and standardized production of traditional active lactobacillus pickles can be realized.

The invention relates to a traditional Chinese medicinal spray for treating bovine foot rot. The traditional Chinese medicinal spray is prepared by extracting cherokee rose fruit, caulis spatholobi, viola japonica, folium artemisiae argyi, rosin, gallnut and dried ginger, and adding a carrier. The traditional Chinese medicinal spray disclosed by the invention is applied to cattle suffering from the disease in a spraying manner twice a day, and is capable of effectively controlling bovine foot rot by continuously applying for 5-7d; and the traditional Chinese medicinal spray is convenient to use, low in cost, free from residue and high in biological safety.

The invention relates to a biological safety protection cabin which is characterized by comprising an upper cabin body, a lower cabin body, a central control system and a negative pressure ventilation purification and disinfection system, wherein the upper cabin body comprises a closed negative pressure cabinet; the front and back sides of the negative pressure cabinet are respectively provided with at least one transparent window and an oversleeve operation port; the left and right sides of the negative pressure cabinet are respectively provided with a transfer window; the negative pressure ventilation purification and disinfection system comprises primary and secondary exhaust high-efficiency particle filters, an exhaust fan, a first tee joint, a pipeline electric closing valve, a second tee joint and a primary blowing high-efficiency particle filter which are sequentially connected through a ventilation pipeline; the third end of the first tee joint is connected with a laboratory exhaust system through an exhaust electric closing valve; the third end of the second tee joint is connected with a blowing fan through a blowing electric closing valve; the middle part of the ventilation pipeline is connected with a gas disinfection plant through a disinfection electric closing valve; an air inlet end of the primary exhaust high-efficiency particle filter is communicated with an air outlet of the upper cabin body; and an air outlet end of the primary blowing high-efficiency particle filter is communicated with an air inlet of the upper cabin body.

The invention relates to a pesticide, in particular to a compound bactericide for mainly treating grey mold of fruits and vegetables. The compound bactericide consists of active ingredients, a filler and / or aids, wherein the active ingredients consist of fluazinam and other bactericides; the weight ratio of the fluazinam to other bactericides is 1-50:1-50; and other bactericides are one or a mixture of more of mancozeb, iprodione, fludioxonil, SYP-Z048, pyrametostrobin and azoxystrobin. Product forms of the compound bactericide for mainly treating the grey mold of fruits and vegetables are usually wettable powder, water dispersible granules and suspension agents. The compound bactericide is applicable to the prevention and control of the grey mold of various fruits and vegetables and has remarkable synergism and long effective duration, the medicaments have a novel action mechanism and a large number of action sites, and the occurrence and development of medicament resistance of grey mold bacteria can be effectively delayed; and the preparation method of the compound bactericide is simple, and the compound bactericide has high biological safety and remarkable economic benefit.

The invention discloses a biodegradable fabric body capable of being developed and a conveying device. A biodegradable mesh grid serves as a main body. The biodegradable fabric body capable of being developed and the conveying device are characterized in that at least two layers of a sealing layer, a development control layer and a promotion endothelial cell growth layer are arranged on the surface of the mesh grid; the biodegradable mesh grid is woven by composite materials which are obtained by one or various biodegradable high molecular materials in a blending or a copolymerization mode; a form of the biodegradable fabric body is geometry composed of one or a plurality of quadric surfaces and comprises a mushroom shape composed of a spherical shape, a double-elliptical external form and double spherical caps and variants thereof; the mesh grid is a single-layer mesh or a double-layer mesh woven by biodegradable silk. After being implanted into hemangioma, the biodegradable fabric body capable of being developed and the conveying device have a shape memory function and enough mechanical strength, can play a role of embolism blocking hemangioma in a short term and are good in sealing performance. Degraded fragments cannot come off to blood to cause an embolism. The biodegradable fabric body capable of being developed and the conveying device have good biological safety, and the possibility that the degraded fragments cause the embolism is lowered.

The invention relates to a method for preparing and dissolving silk fibroin powder, which comprises the steps of degumming, pre-alkali washing, high-temperature high-pressure treatment, purification, pre-concentration, drying, and ultrafine grinding. The method of the invention adopts a high-temperature high-pressure method combined with ultralow-concentration alkali liquor to dissolve fibroin fibers, can completely dissolve fibroin fibers, and breaks the precedent that a high-temperature high-pressure method cannot dissolve silk fibroin; when compared with other technology in the prior art, the method adopts alkali liquor with an ultralow concentration, does not destroy the internal structure of natural silk fibroin, is simple and high-efficient in process, has significantly decreased cost, is short in production period, high in biological safety, simple and convenient, does not generate other wastes, and causes no environment pollution.

The invention discloses a method for sterilizing water by ultraviolet light catalysis and chlorine. The water treatment method combines ultraviolet light catalysis sterilization and chlorine sterilization to sterilize water. The method comprises the following steps: sterilization with the ultraviolet light catalyst is carried out first, and then the chlorine is added to the water within 0-60 minutes for the sterilization, or the chlorine is added to the water for the sterilization first, and then the sterilization with the ultraviolet light catalyst is carried out within 0-60 minutes, or the sterilization with the ultraviolet light catalyst is carried out on the water while adding the chlorine to the water for the sterilization, or the chlorine is previously added to the water before coagulation treatment is carried out on a water sample, coagulation treatment, precipitation treatment and filtration treatment are carried out, and then the sterilization with the ultraviolet light catalyst is carried out for the sterilization, or the ultraviolet light catalysis sterilization and the chlorine sterilization is carried out for treating the water sample after the coagulation treatment, the precipitation treatment and the filtration treatment. The method ensures biological safety and chemical safety of the water sample.

The invention provides a magnesium-zinc-copper alloy. The magnesium-zinc-copper alloy comprises 1-8wt% of zinc, 3wt% or less of copper, and the balance of magnesium. The magnesium-zinc-copper alloy has good biocompatibility, satisfactory degradability, high biological safety and extremely good biomechanical performances, can continuously slowly release metal ions having an antibiotic effect to surrounding tissues through in vivo degradation in order to effectively prevent and treat implant surrounding infection, has certain hematopoietic activity, and has good application values in implanting into bones and other medical fields.

The invention discloses an intelligent aquarium fish tank. According to the intelligent aquarium fish tank, the water temperature, illumination, pH, dissolved oxygen and other factors of a water quality environment can be automatically controlled, a simulated artificial raising ecological environment is provided, automatic cleaning and refreshing of the water environment in the fish tank are realized, and actually the goal of avoiding water change for a long term is achieved. The intelligent aquarium fish tank is also provided with an automatic feeding system and a health and bright body color monitoring analysis system so that raised organisms can keep a healthy and colorful state, and the fun of a raiser is enhanced. The intelligent aquarium fish tank is characterized in that in the fish culture facility, a device is composed of a plurality of digital intelligent control systems. The intelligent aquarium fish tank has the advantages of environmental intelligence controllability, ecological and healthy raising (biological safety), automatic regulation, energy conservation and the like and is an important sign of modern technical breeding.

The present invention provides an improved bio-safety cabinet having a microprocessor control system for the monitoring and control of the inflow, downflow and outflow of air, for creation of a performance envelope within the work chamber wherein all contaminated areas are under negative pressure or surrounded by negative pressure and the work chamber is continuously bathed in clean air, ensuring the microbiological work is protected form contamination in normal room air. Improved decontamination and other safety measures for a bio-safety cabinet are also proposed.

The invention relates to a pesticide, in particular to a complex bactericide for mainly treating grey mould disease, early blight and powdery mildew, which is used for preventing and controlling the grey mould disease, early blight, powdery mildew, downy mildew and late blight of vegetable and fruit crops. The complex bactericide consists of active ingredients, a filler and / or an auxiliary agent, wherein the active ingredients consist of boscalid and methoxyl acrylate bactericide in the weight ratio of 1-100:1-100. The complex bactericide can also be prepared into wettable powder or water dispersible granules for use. The complex bactericide has the advantages of high quick acting performance, novel action mechanism and capability of effectively suppressing and delaying the occurrence and development of germ resistance to the medicament; and a preparation process has the advantages of simplicity, low cost, wide disease prevention spectrum and high biological safety.

The present invention provides a microbial sample processing device, comprising: a machine body provided with a machine chamber and a sealed movable door; and a sealed housing, a stretchable airbag, a sample tray, a mechanical arm, a bar code scanner, a liquefier, culture dishes, a gas-temperature sensor, and a temperature control device which are arranged in the machine chamber, wherein the sample tray is provided with a plurality of sample seats, the sample seats are used for placing the culture dishes, the liquefier is communicated with the culture dishes, each culture dish is provided with culture plates and a streak-inoculation pen used for streak inoculating the sample in the culture dishes from the liquefier on the culture plate. The microbial sample processing device is reasonable in structure, fast and safe, reliable and efficient, can complete the whole process of mixing and liquefying, streak inoculation, dilution and separation, and incubation of microbial community automatically in a one-stop manner, and can replace manual labor and ensure biological safety.

The invention discloses a method for preparing fermented product filtrate by fermenting rice with lactobacillus casei, and the application of the fermented product filtrate. The method comprises the steps of firstly, carrying out heat sterilization on a culture medium; then, inoculating the lactobacillus casei into the culture medium, fermenting, and filtering to obtain the fermented product filtrate; after that, adjusting the pH value of the fermented product filtrate, and sterilizing to obtain the fermented product filtrate. After being used in the compositions of cosmetics, the fermented product filtrate has a good function of promoting the permeation of other components, the skin wrinkles can be reduced, and the skin melanin is reduced; the fermented product filtrate is high in biological safety, thus being widely applied to various cosmetics.

The invention relates to a reactor and a method for removing ammonia nitrogen in drinking water by virtue of an enhanced filter. The reactor is additionally provided with a biological nitrification region provided with a biological filler, and an aeration system under the circumstance that the previous water feed region, water distribution region, bearing layer and water discharge region of a sand filter are not changed; the added biological nitrification region and the previous filtering region of the sand filter are located in the same reactor and form a reaction region, the biological nitrification region is located above the previous filtering region of the sand filter, and an aeration head communicated with an air compressor of the aeration system by virtue of an air pipe is mounted under the biological filler. The reactor is capable of removing ammonia nitrogen and partial organic matters in water by virtue of enhanced nitrification of a microbial film grown on the surface of the biological filler and providing oxygen needed by organisms by virtue of aeration; in the filtering process, suspended particles, the microbial film and the like in water are intercepted, so that the biological safety is guaranteed and secondary pollution is prevented. The reactor and the method can be applied to purification treatment of drinking water such as an ammonia nitrogen polluted water source.

The invention provides bacillus subtilis, a biofilm and construction and application thereof. A biofilm exhibition platform is a modified biofilm of the bacillus subtilis, wherein a TasA protein in the bacillus subtilis biofilm is modified into a TasA-R protein, is exhibited on the biofilm and has functionality. Gene modification and regulation and control of a main protein of the bacillus subtilis biofilm, namely a TasA starch sample protein, are carried out, and the exhibition platform of the bacillus subtilis biofilm is realized; compared with previous reports, the platform has the advantages of higher secretion capability, more complete functionality, higher biological safety, capability of being regulated and controlled and the like; the exhibition technology of the bacillus subtilis biofilm is widely applied.

The invention discloses a recombinant low-virulent vaccine strain of chicken infectious bursal disease viruses (IBDV) and application thereof. In the invention, a major protective antigen gene VP2 of an epidemic superhigh virulent strain is cloned, the nucleotide of the gene VP2 is modified by mutation and then used for replacing a corresponding segment of a Gt genome of a low-virulent strain of the IBDV, so that the infectious clone of a recombinant genome of the IBDV is constructed, and the recombinant low-virulent vaccine strain is saved and identified by using an IBDV reverse genetic operation system. The microbial collection number of the vaccine strain is CGMCC No.3749. The recombinant low-virulent vaccine strain of the invention has high replicability, genetic stability and safety. The immune effect of the low-virulent vaccine strain of the invention is as good as that of the medium-virulent vaccine strain, but is superior to that of the low-virulent vaccine strain. The biological safety of the low-virulent vaccine strain of the invention is superior to that of the medium-virulent vaccine strain. As the vaccine strain, the recombinant low-virulent vaccine strain of the invention has the characteristics of high efficiency and low toxicity, is a good candidate vaccine strain and can be used for controlling chicken infectious bursal disease.

The present invention provides a magnesium-copper alloy, which contains magnesium and copper, wherein the copper content is less than or equal to 3 wt%, and the balance is the magnesium. According to the present invention, the magnesium-copper alloy has characteristics of good biodegradability, satisfactory degradability, high biological safety, and excellent biomechanical property, has application values in medical fields of endosseous implants and the like, especially continuously and slowly releases metal ions with the bacterial inhibiting effect to the surrounding tissue through in vivo degradation, has effects of effective prevention and treatment of infections surrounding implants, and has a certain angiogenesis activity.

The invention relates to a medicinal oxygen-containing beta titanium alloy for orthopedic implantation and a preparation method thereof, which belong to the technical field of medicinal apparatus and instruments. The preparation method comprises the following steps of: performing vacuum self-consuming smelting treatment on alloy ingredients to obtain a titanium-niobium-zirconium-tantalum (Ti-Nb-Zr-Ta) alloy; performing non-consumable smelting treatment on the Ti-Nb-Zr-Ta alloy; and then performing heat treatment and heat processing sequentially to prepare the medicinal beta titanium alloy and obtain the medicinal oxygen-containing beta titanium alloy, wherein the oxygen element accounts for 0.13 to 0.53 weight percent of the medicinal oxygen-containing beta titanium alloy, and the titanium-niobium-zirconium-tantalum alloy comprises the following components in percentage by mass: 60 percent of Ti, 35 percent of Nb, 3 percent of Zr and 2 percent of Ta. In the preparation method, elements with biological safety are selected and a high-content beta stable element is added, so the alloy has high cold machining property; and the alloy is strengthened by increasing the oxygen element content in the alloy and the oxygen element ratio is optimized by adding the oxygen elements with different content, so the alloy has high strength and low elastic modulus.

An improved system and method for monitoring contamination loading of a filter in a biological safety cabinet comprising a housing defining a work chamber and a filtration chamber, a system for circulating air between the work chamber and the filtration chamber via a fan which draws air under negative pressure from the work chamber and delivers the air under positive pressure through the filter and into the filtration chamber. The filter monitoring system determines a pressure differential between the negative and positive air pressure at opposite sides of the fan, and evaluates the degree of contamination loading of the filter on the basis of the pressure differential. An air flow baffle is disposed within the filtration chamber adjacent the fan for dividing the pressurized air delivered by the fan and partially redirecting a portion thereof for more uniformly delivering the air to the filter.

The invention discloses a technology for controlled synthesis of polylactic acid through a lactide active ring-opening polymerization reaction under the catalytic action of organic guanidine-nontoxic alcohol serving as a catalyst. According to the technology, nontoxic organic RCOOCG is adopted as the catalyst, nontoxic organic alcohol is adopted as an initiating agent, and the lactide active ring-opening polymerization reaction is performed according to a noumenon fusion and polymerization method. The technology is characterized in that the catalyst activity is high; the temperature of the polymerization reaction is low; the reaction time is short; a polymer product, namely polylactic acid, can be subjected to controlled synthesis within a range (Mn: 0.5*10<4>-5.0*10<4>) of the actual application requirement for the number-average molecular weight; the molecular weight distribution of the polymer product is narrow (PDI: 1.10-1.25); no monomer is remained in the polymer product (monomer conversion rate: 100%); the polymer product is snow-white in color and high in biological safety.

The invention relates to a liquid dressing containing a chitosan derivative, comprising the following components in percentage by weight: 1-5% of CMCS (carboxymethyl chitosan), 0.1-0.5% of collagen albumen powder, 10-15% of humectant, 0.2-2% of antibacterial agent, 2-10% of penetration enhancers and the balance of deionized water. The liquid dressing is obtained by mixing the components in sequence, stirring evenly and bulking. The liquid dressing has the advantage of good biocompatibility, good antimicrobial, antiphlogistic and hemostatic effects and high biological safety, and is convenient to use.

The invention provides a cell reactor and an artificial liver support system comprising the same. The cell reactor comprises a container, a liquid outlet is formed at the bottom of the container, a sealing cover is arranged at the top of the container, a liquid inlet is formed on the sealing cover, layers of filter meshes of 100-400 meshes are respectively arranged at the bottom of the container and in the sealing cover at the top of the container, covers which are in seal fit with the liquid outlet and the inlet are formed at the liquid outlet and the inlet, and a mixture of a micro-carrier growing liver cells and resin is accommodated between the two layers of the filter meshes in the container. The reactor is connected on a dialysate circulation path of a blood purifying device for constituting the artificial liver support system, thereby leading the liver cells not to be in direct contact with blood and realizing good biological safety; furthermore, the reactor can be used for a plurality of times through regeneration treatment, thereby greatly reducing the treatment cost of a biological artificial liver.

The invention relates to a piglet protein feed and a production method thereof, which belong to the technical field of herding feed processing technology. In a method for removing anti-nutritional factors of soybean or soybean residue, hot-press puffing is adopted firstly, and then aerobic fermentation and anaerobic fermentation are carried out. The method can remove the anti-nutritional factors in low-temperature desolventized soybean residue and raw soybean, overcomes the serious defect that the anti-nutritional factors are used as the main protein nutrition sources for the piglets, and promotes the production of a large number of easily digestible functional nutrients and flavor components. The soybean residue material fermented grains and soybean meal material fermented grains of the obtained anti-nutritional factors are the piglet protein feed with good quality, and are used for preparing full-price daily ration for piglets with the ages of 7-70 days, the adding proportion is 15-30%, the feed replacing the soybean residue increases the daily gain by 10.5%, reduces the diarrhea rate by 30%, and increases the survival rate by 8%; and the feed replacing imported fish meal, proteinic powder of porcine corpuscle and other animal-based protein feeds reduces the daily ration cost by 15-30% and increases the biological safety of the feed.