694 results about "Biological safety" patented technology

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention discloses a bacteriostatic and antiviral bamboo pulp paper-based sanitary isolation bag as well as a preparation method and application thereof, and belongs to the field of functional paper-based packaging materials. The isolation bag takes bamboo pulp fiber paper as a base material, and the surface of the isolation bag is coated with a water-based polyhydroxyalkanoate functional coating. According to the preparation method, a special polyphenol derivative-chitosan quaternary ammonium salt-nano zinc oxide organic-inorganic hybrid factor is introduced, and the problem of agglomeration of nano particles is effectively solved by utilizing the special bridging effect of polyphenol, so that high-density cation capture and oxidation inactivation sites are constructed on the surface of the coating. Compared with the prior art, the method has the advantages that the method does not need to depend on organic fluorine compounds, and the excellent waterproof and oil-proof barrier effect and the efficient inactivation of viruses can be achieved only through the extremely low coating amount. In addition, the isolation bag has unique environmental chemical responsiveness, can be efficiently dissociated under the alkaline condition to facilitate repulping recovery, can be completely biodegraded in the natural environment, and perfectly considers the requirements of biological safety protection and green environmental protection.

The present application relates to the technical field of aquaculture monitoring and regulation, and discloses a water quality multi-parameter real-time online monitoring system. The system comprises a multi-source data acquisition module for obtaining and denoising water quality data; a time sequence feature analysis module for generating multi-dimensional feature clusters containing the correlation of various parameters; an anomaly identification module for dynamic deviation compensation, monitoring sub-domain demarcation and key parameter extraction; a collaborative regulation module for constructing a regulation decision unit to generate a multi-parameter collaborative regulation strategy; and an iterative correction module for correcting the regulation strategy to output real-time adjustment instructions. An early warning module is also provided for biological safety assessment and early warning and activation of the emergency intervention mechanism. The system can accurately monitor the multi-parameters of the water quality of aquaculture in real time, realize intelligent regulation and biological safety early warning, effectively improve the yield and quality of aquaculture, reduce the risk of aquaculture, and promote the sustainable development of the industry.

The invention discloses design and synthesis of a series of brain-targeted ginsenoside Rg1 derivatives and application of the brain-targeted ginsenoside Rg1 derivatives in treatment of Alzheimer's disease. According to the invention, ginsenoside Rg1 and polyethylene glycol with a specific chain length are covalently linked to successfully construct the derivative capable of enhancing the penetrating power of the blood-brain barrier. The series of derivatives provided by the invention not only solve the problems of poor brain targeting and insufficient stability of natural Rg1, but also can inhibit neuroinflammation by regulating and controlling an NLRP3 / caspase-1 signal channel in an LPS-induced neuroinflammation model, and finally, the learning and memory ability is remarkably improved. The product is simple and convenient in preparation process and good in biological safety, and a new candidate compound is provided for developing a new generation of Alzheimer disease treatment medicines.

The invention relates to the field of microbiological detection, in particular to a method for detecting vibrio parahaemolyticus by combining RPA-CRISPR / Cas12a with a microfluidic biosensor. According to the method, the preloaded freeze-drying reagent is combined with centrifugally driven liquid operation, so that rapid and closed system detection is realized, and the pollution risk is effectively reduced. According to the invention, quantitative detection of vibrio parahaemolyticus is realized in a centrifugal micro-fluidic chip by systematically optimizing an RPA amplification primer and crRNA. The detection method shows high specificity and high sensitivity to non-target vibrio and other food-borne bacteria, and the lower detection limit is 6.08 copies / mu L. In a word, the invention provides a rapid, portable and sensitive platform, can realize quantitative detection in a wide linear range, provides a practical tool for real-time monitoring of pathogens, and has application prospects in monitoring of aquaculture biosafety and marine product safety.

The invention belongs to the technical field of drug delivery, and particularly relates to an M-coated PLGA-10BX compound as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing 10B-enriched boron nitride (h-10BN); extracting a cell membrane of the macrophage RAW264.7; polylactic acid-glycolic acid copolymer (PLGA) nano particles loaded with h-10BN are prepared; and finally, the bionic nano platform M (at) PLGA-10BN based on the macrophage membrane is prepared. The bionic nano-platform prepared by the invention is uniform in particle size and morphology, has relatively high biological safety, and has no obvious damage to various tissues and visceral organs. The compound can be used as a general platform for boron compound delivery, can also be used as an immune activator, is suitable for intravenous injection administration, and expands the application of boron neutron capture therapy (BNCT) in treatment of glioblastoma (GBM).

A system, method, and receiver control architecture for a Symbolic Resonant Energy Transfer Network (SRETN) configured to distribute electrical power wirelessly across planetary-scale distances under cryptographic, economic, and biological safety governance. The system comprises a distributed mesh of terrestrial resonant transmission towers and orbital relay satellites configured to deliver energy via directed magnetic resonance coupling. A Symbolic Energy Kernel governs authorization and transmission, preventing energization of any transmission vector unless a secure cryptographic energy handshake is completed. The handshake verifies: (i) authenticity and compliance of a requesting receiver device; (ii) real-time solvency of an associated decentralized energy ledger account; and (iii) clearance of the transmission path through active radar and lidar-based biological exclusion scanning. Energy is transmitted as discrete, tokenized packets, each cryptographically metered and acknowledged by the receiver prior to continuation. The system enables a wireless, infrastructure-light energy distribution paradigm in which electrical power is accessed on demand with transaction-level auditability and enforced safety constraints.

The present invention relates to a dental diaphragm copolyester and a preparation method thereof, the preparation method comprises the following steps: 2, 5-furandicarboxylic acid or an ester thereof, aliphatic or alicyclic diol and (2-hydroxy-5-formyl phenyl) diphenylborane are subjected to a reaction to obtain an esterification or ester exchange product; and carrying out condensation polymerization on the esterification or ester exchange product and the stabilizer to prepare the copolyester for the dental diaphragm. 2, 5-furandicarboxylic acid is derived from a renewable biomass resource and meets the requirements of green and sustainable development; compared with terephthalic acid and furan ring structures, the film has higher rigidity and polarity, the yield of hot press molding is improved, the same correction force can be met by a thinner film, the wearing comfort of a patient is improved, and meanwhile stress relaxation is improved; the catalyst perfectly combines strong Lewis acidity, intramolecular stabilization and potential bifunctional activation through structural design, provides possibility for efficient and green synthesis of polyester, avoids use of heavy metal, and is higher in biological safety.

The invention discloses a multi-target joint optimized antibiotic molecule design system and method applied to the field of biological medicine, and the method comprises the steps: obtaining FabI enzyme and AcrAB-TolC efflux pump structure data, extracting double-target space and physicochemical characteristics, and constructing a joint pharmacophore model containing a FabI inhibition domain and an efflux pump blocking domain; screening candidate molecules meeting a double-domain space matching threshold, screening high-affinity double-target molecules through conformation sampling and free energy calculation, and forming a lead compound library through multi-layer filtration of metabolic stability, membrane penetrability, quantum chemical properties and synthesis feasibility; carrying out electron effect, chain length or heteroatom fine tuning on dominant molecules through in-vitro bacteriostasis and efflux inhibition experiment feedback, and carrying out iterative optimization until MIClt is met; 2 [mu] g / mL and the efflux inhibition rate is gt; according to the present invention, the antibacterial effect and the drug resistance inhibition ability are significantly improved, the molecular synthesizability and the biological safety are ensured, and the engineering design new path is provided for the Gram-negative bacterium drug resistance crisis.

The invention belongs to the technical field of medical wound materials, and relates to conductive nanofiber aerogel for preventing and treating stress injury and a preparation method thereof. The conductive nanofiber aerogel comprises nanofiber aerogel, a polypyrrole conductive network and a polydopamine hydrophilic layer, wherein the polypyrrole conductive network and the polydopamine hydrophilic layer are deposited in the nanofiber aerogel; the nanofiber aerogel is composed of nanofibers containing a first methylacryloylated polymer and polylactic acid and a second methylacryloylated polymer, the nanofibers are in staggered lap joint to form a three-dimensional skeleton, and the lap joint is wrapped by the second methylacryloylated polymer. The preparation method comprises the following steps: preparing nanofiber aerogel, and sequentially infiltrating the nanofiber aerogel into a pyrrole / FeCl3. 6H2O solution and a dopamine hydrochloride / Tris-HCl solution to react. The conductive nanofiber aerogel has good structural stability, biological safety, hydrophilic imbibition and pressure / seepage sensing capability, and can play an efficient role in prevention and treatment of pressure injury.

The invention discloses a mycobacteriophage and application thereof, and relates to the technical field of bacteriophages. The mycobacteriophage is named as Mycobacter phage BZNK001, is preserved in Guangdong Microbial Culture Collection Center, has a preservation number of GDMCC No: 67907-B1, does not contain antibiotic drug-resistant genes and integrase genes, has excellent biological safety and good environmental tolerance, can maintain high biological activity in a temperature range of 37-50 DEG C under an acid-base condition of pH 5-9, and can be used for preparing the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage for preventing and treating the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage for preventing and treating the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage. The bacillus subtilis has broad-spectrum and high-efficiency non-tuberculous mycobacterium cracking activity, and can effectively crack four clinical key mycobacteria such as mycobacterium abscessus, mycobacterium avium, mycobacterium intracellularis and mycobacterium smegmatis. According to the invention, a mycobacteriophage resource library is enriched, a novel and stable biological prevention and control means is provided for efficient prevention and control of mycobacteria, and the mycobacteriophage has good clinical application and industrialization values.

The present application relates to a kind of pH / H2O2 dual-response drug-loaded hydrogel and its preparation method and application, by ε-polylysine derivative as antitumor active ingredient, with oxidized dextran through Schiff base reaction crosslinking rapid gelation. While using 2-formylphenyl boronic acid covalently package CD73 small molecule inhibitor APCP. ε-polylysine derivative has oncolytic activity, can induce ICD and promote the infiltration of immune cells in tumor cells. CD73 small molecule inhibitor APCP can block extracellular CD39-CD73-adenosine pathway, reduce the level of immunosuppressive metabolite adenosine in tumor tissue, so as to activate the infiltrated immune cells, restore its anti-tumor function. The hydrogel of ε-polylysine derivative and APCP co-delivery synergistically inhibits the effect of tumor growth, and its curative effect is greater than the treatment effect of single drug, and is superior to positive control drug oxaliplatin (OXA). The pH / H2O2 dual-response hydrogel is rapidly gelled, and has good biological safety under mild reaction conditions.

The invention relates to the technical field of medical nano-materials, and discloses a pH-responsive drug self-delivery nano-system and a preparation method and application thereof.The preparation method comprises the steps that 1, CMNPs is prepared, specifically, through an amidation reaction, the CMNPs are prepared from methotrexate and cis-aconitic anhydride; step 2, synthesizing DSPE-PEG-T12: synthesizing the DSPE-PEG-T12 by virtue of a nucleophilic addition reaction; step 3, preparing a T12 peptide modified erythrocyte membrane; and step 4, preparing T12-RBCM (at) CMNPs: modifying the T12 peptide of the targeted tumor cell transferrin receptor on an erythrocyte membrane, and coating the CMNPs to construct the nano-acquisition system T12-RBCM (at) CMNPs. According to the nano system T12-RBCM (at) CMNPs obtained by the scheme, the tumor cell uptake efficiency can be remarkably improved, cell apoptosis is induced, and proliferation is inhibited; the long-acting circulation and tumor targeting capability is realized, the tumor growth can be effectively inhibited, and the biological safety is good.

The invention discloses an antibacterial peptide PA targeting multi-drug-resistant gram-negative bacteria and application of the antibacterial peptide PA, and the amino acid sequence of the antibacterial peptide PA is shown as SEQ ID NO.1. The antibacterial peptide PA provided by the invention has a remarkable antibacterial effect of the multi-drug-resistant gram-negative bacteria, is quick in bactericidal effect, does not have hemolysis and cytotoxicity, does not cause renal toxicity after treatment, and can be used for preparing the antibacterial peptide PA for treating the multi-drug-resistant gram-negative bacteria. The biological safety is good, and the compound can be used for treating bacterial infection and can also be applied to other scenes needing sterilization or bacterial growth inhibition.

The invention provides a radix asparagi homogeneous acidic polysaccharide. The radix asparagi homogeneous acidic polysaccharide contains pectic polysaccharide which is shown as a formula 1, takes rhamnogalacturonidan I (RG-I) as a core and has a homopolygalacturonic acid (HG) structural domain. The invention further provides a preparation method and application of the radix asparagi homogeneous acidic polysaccharide. The asparagi pectin polysaccharide with a new structure is found for the first time, and the asparagi pectin polysaccharide has the effect on acute lung injury. The acidic polysaccharide disclosed by the invention has the advantage of anti-ALI activity, realizes synergistic protection of intestine-lung double target regions, has anti-inflammatory and anti-oxidation double activities, is natural in source and high in biological safety, and is suitable for long-term adjuvant therapy of acute lung injury.

The invention discloses a PDRN-loaded umbilical cord mesenchymal stem cell source nano-vesicle and a preparation method thereof, and belongs to the technical field of biological medicines. The preparation method provided by the invention comprises the following steps: mixing an umbilical cord mesenchymal stem cell suspension with a PDRN solution, and carrying out freeze thawing treatment; incubating the mixed solution subjected to freeze thawing treatment; extruding the incubated mixed solution for multiple times by using a filter membrane; and collecting the extruded product, and separating and purifying to obtain the PDRN-loaded umbilical cord mesenchymal stem cell source nano-vesicle. According to the preparation method disclosed by the invention, the encapsulation efficiency of PDRN is remarkably improved, meanwhile, secondary damage of a traditional post-loading technology to a preformed vesicle structure is avoided, the membrane integrity and biological activity of a final product are better maintained, the yield is high, and the preparation process is rapid; the introduction of exogenous impurities is avoided from the source, and the high purity and biological safety of the obtained nano-vesicle component are ensured.

The invention belongs to the technical field of biological medicine, and particularly relates to a chitosan modified gold nanocluster, a preparation method and application in red blood cell immobilization. According to the synthesis method of the CS-GSH-AuNCs cluster, a two-step low-temperature modification method is utilized, GSH-AuNCs core nanoparticles with a definite structure are synthesized firstly, then chitosan is modified through electrostatic interaction, the reaction condition is low temperature and nitrogen protection, the reaction condition is mild, the product repeatability is high, and the original structure of GSH-AuNCs is more complete. Experiments show that the CS-GSH-AuNCs cluster synthesized by the method has good acid and alkali tolerance and temperature tolerance, the effect of immobilizing red blood cells is good, and the biological safety is high.

The invention relates to the technical field of medical protection materials, and discloses an anti-allergy skin-friendly operating kit and a preparation technology, and the composite material of the operating kit comprises the following components by mass: 40-70 parts of cellulose-based non-woven fabric; 1 to 10 parts of polymethacryloyloxyethyl phosphorylcholine which is covalently grafted to the cellulose-based non-woven fabric; 20 to 50 parts of polylactic acid-quaternary ammonium salt copolymer; 5 to 15 parts of a polyurethane hot melt adhesive; the method comprises the following steps: grafting a PMPC molecular brush on the surface of a cellulose-based non-woven fabric to obtain a skin-friendly layer; carrying out coaxial electrostatic spinning and solvent treatment on the polylactic acid-quaternary ammonium salt copolymer to prepare a hollow nanofiber barrier layer; and finally hot-pressing and compounding the two layers through a polyurethane hot-melt adhesive. According to the invention, allergy is solved through surface grafting; the toxicity is avoided by using an intrinsic antibacterial polymer; the hollow fibers and the net film are compounded, so that the contradiction between water resistance and breathability is overcome, and the biological safety and comfort of the material are improved.

The invention discloses a pasteurella phage PTP1APTYT15 as well as a composition, a kit and application of the pasteurella phage PTP1APTYT15, the pasteurella phage PTP1APTYT15 is preserved in Guangdong Microbial Culture Collection Center on September 10, 2025, the preservation number of the pasteurella phage PTP1APTYT15 is GDMCC NO: 66946-B1, and the pasteurella phage PTP1APTYT15 is classified and named as Pasteurella phage PTP1APTYT15. The pasteurella phage PTP1APTYT15 provided by the invention has the characteristics of high temperature resistance and alkali resistance, and can be used for cracking pasteurella of various serotypes. In addition, the bacteriophage is high in proliferation efficiency and high in splitting capacity, has no adverse effect on human or animal health, and has good biological safety.

The invention discloses application of beta-nicotinamide mononucleotide in regulation and control of gene editing efficiency, belongs to the field of gene editing treatment, and finds that the beta-nicotinamide mononucleotide (NMN) can efficiently inhibit the activity of CRISPR-Cas9, CRISPR-Cas12 and CRISPR-Cas13 systems in a broad-spectrum manner for the first time. The application comprises emergency blocking of off-target effect in gene editing clinical treatment, biological safety prevention and control of a virus vector gene editing system, and CRISPR activity regulation and control of in-vitro non-diagnostic purpose. Experiments show that NMN can inhibit CRISPR-mediated gene damage and cell death in a cell model, the inhibition efficiency in an in-vitro enzyme digestion system reaches 68.7%, and cell growth or transfection efficiency is not affected. The invention provides an innovative solution for safe application of CRISPR (clustered regularly interspaced short palindromic repeats) technology, and the NMN is approved to be taken orally as a health care product, so that the NMN has extremely strong clinical application potential. Compared with the existing CRISPR (clustered regularly interspaced short palindromic repeats)-resistant protein or synthetic small-molecule inhibitor, the NMN has the advantages of endogenous property, high biocompatibility, good oral safety and the like.

The invention discloses a human-derived hysteromyoma targeting peptide and application thereof, the sequence of the targeting peptide is selected from SEQ ID NO: 1 to SEQ ID NO: 5, and the SEQ ID NO: 1 (VN12) is the most preferable. The fusion protein is obtained by screening through a phage display technology, and has high hysteromyoma targeting specificity and low immunogenicity. The targeting peptide can be coupled with nuclide or fluorescent dye, and is used for accurate PET imaging, fluorescent diagnosis and intraoperative navigation of hysteromyoma. Experiments prove that the molecular marker is specifically enriched in tumor tissues (the tumor / muscle ratio is 60), the biological safety is good, the problems of insufficient diagnosis sensitivity and large treatment wound in the prior art are solved, and the molecular marker has a great application prospect in diagnosis and treatment of hysteromyoma.

The application relates to a bag-in-bag-out filtering device, which relates to the technical field of biological safety and comprises the following: a ventilation duct is arranged in a box body; an air inlet and an air outlet are arranged on the box body and communicate with the ventilation duct; one or more filters are movably arranged in the ventilation duct; an opening is arranged on the box body and communicates with the ventilation duct; a sealing door is detachably arranged on the opening; a detection piece is arranged on one side of the box body close to the sealing door; and a frame body is arranged in the box body and abuts against the filter; the box body comprises a main body, an air inlet shell, an air outlet shell and a cover plate, and the ventilation duct is formed between the main body and the cover plate; the frame body and the main body are integrally bent and formed, one end of the frame body is embedded in the main body, and the other end of the frame body is bent and connected with the end of the main body. Compared with a traditional filtering box, the filtering device is integrally formed, welding leakage is avoided, the filtering device is not easy to deform, good sealing performance and pressure bearing strength are maintained, and the cost is reduced.

The invention provides a stepped directional extraction method of fucoidan, and relates to the technical field of extraction of active ingredients of algae. The method comprises the following steps: pretreating brown algae powder by microwaves and ultrasonic waves, mixing the pretreated brown algae powder with a deep eutectic solvent, and carrying out ultrasonic extraction to obtain algin; the eutectic solvent is composed of choline chloride and lactic acid according to the molar ratio of 1: (1-3). According to the method disclosed by the invention, a microwave-ultrasonic synergistic wall-breaking coupling deep-eutectic solvent extraction process is adopted, so that the yield of algin is increased to 73.2%, meanwhile, an additional toxic chemical chelating agent does not need to be added, the effect of effectively adsorbing heavy metal ions is achieved, and the biological safety and commercial value of the product are greatly improved.

The invention relates to the technical field of bioengineering, in particular to a preparation method and application of an antibacterial peptide, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID No: 1 and is named as L8. The preparation method of the antibacterial peptide L8 comprises the following steps: S1, obtaining an L8 target gene segment; connecting an L8 target gene segment with a pLC vector to obtain a connection product; transforming the connection product into an escherichia coli DH5-alpha competent cell, and screening positive clones to obtain a pLC-L8 recombinant plasmid; s2, taking the pLC-L8 recombinant plasmid as a substrate, and carrying out cell-free protein synthesis reaction to obtain the antibacterial peptide L8, the cell-free protein synthesis system comprises 175 mM of potassium glutamate, 10 mM of ammonium glutamate, 2.7 mM of potassium oxalate, 33 mM of PEP, 38 mM of compound amino acid, 10 mM of magnesium glutamate, 2% (w / v) PEG-8000, 5 nM of pLC-L8 recombinant plasmid, 11.25 mM of lactose, 33.33% (v / v) of crude extract, 100 mg / mL of escherichia coli tRNA and 2.5 nM of T7 RNA polymerase. The antibacterial peptide L8 is obtained by optimizing the antibacterial peptide LRGG, and compared with the LRGG, the antibacterial peptide L8 subjected to cell-free protein synthesis reaction expression has better antibacterial activity and biological safety.

The invention discloses an intelligent monitoring method, device and equipment for livestock breeding and a storage medium, and relates to the technical field of intelligent monitoring of livestock breeding, and the method comprises the steps: S100, data collection and time synchronization; s200, carrying out adaptive denoising and cross-modal feature extraction; s300, carrying out individualized baseline modeling and drift self-adaption; s400, performing multi-scale event association and weak causal identification; s500, risk grading and executable intervention suggestion generation; according to the method, priority disposal decision and resource optimization are oriented to economic and biological safety, namely, path confidence scores and node influence probabilities are integrated into calculation of influence ranges and expected intervention cost, and confidence-driven uncertainty premium is reflected in the cost, so that alarms not only reflect biological risks, but also reflect uncertain premium in the cost; the potential economic influence and the operation cost can be quantified; based on the multi-dimensional score, the method can preferentially put limited veterinarians and epidemic prevention resources, medicines and isolation measures into a cluster which contributes to group health and productivity loss risk to the greatest extent.

The invention discloses application of bacillus subtilis ZY56 in prevention and treatment of plant soil-borne diseases, and belongs to the technical field of plant protection. In order to solve the problem that in the prior art, efficient, safe and environment-friendly prevention and treatment measures are lacked in production of soil-borne diseases, the invention provides a strain of bacillus subtilis ZY56 with the preservation number of CGMCC No. 36896; the bacillus subtilis ZY56 provided by the invention has a broad-spectrum antagonistic effect on various soil-borne diseases, also has growth promoting potential, has the characteristic of high biological safety, is harmless to the environment, people, livestock and crops, and meets the requirements of green agriculture and sustainable development.

The application relates to a light-degradation-driven high-molecular nanomotor and a preparation method, and aims to solve the environmental pollution and biological safety problems caused by the degradation difficulty or long cycle of the nanomotor power assembly. The nanomotor has an asymmetric nanostructure and is assembled by a light-degradation high-molecular assembly and a biocompatible high-molecular effective load in a nanometer scale. The preparation method comprises the following steps: 1. uniformly mixing a light-degradation high-molecule and a biocompatible high-molecule in an organic solvent to prepare a mixture A; 2. mixing the mixture A and water at a high speed to prepare a mixture B; and 3. removing the organic solvent to prepare the nanomotor. Under light, the light-degradation assembly absorbs light energy to degrade and generate bubbles, which push the nanomotor to move in the solution. The start / stop of the nanomotor is controlled by the on / off of the light source, and the fast / slow movement of the nanomotor is controlled by the strong / weak of the light source. The structure of the light-degradation assembly is gradually degraded and finally completely destructed with the reaction, so that the biological safety and environmental pollution problems of the nanomaterial are relieved.

The invention belongs to the technical field of biological medicine, and particularly relates to a nano vaccine based on GD2 mimetic peptide as well as a preparation method and application of the nano vaccine. The nano vaccine provided by the invention comprises a GD2 mimetic peptide, an STING agonist and a polymer carrier, wherein the STING agonist is used as an immunologic adjuvant. The antigen peptide (GD2 mimic peptide 47-LDA) and the adjuvant (STING agonist) are wrapped in the micelle to prepare the nano vaccine GD2-NV, so that the druggability of the antigen peptide and the STING agonist is improved, and the accumulation of drug tumors and drainage lymph node parts is improved. Besides, the nano vaccine can respond to a lysosome acid environment, realize lysosome escape, enhance antigen cross presentation of DC, activate an STING pathway, improve infiltration and activation degrees of CD8 + T cells, induce lasting humoral immune response and inhibit growth and recurrence of tumors, and is good in biological safety.

The invention discloses a water-soluble silk fibroin with a random crimp / Silk I structure as a main structure as well as a preparation method and application of the water-soluble silk fibroin. The silk fibroin product is characterized in that in a protein secondary structure, the total content of random curls and Silk I structures exceeds 85%, and the proportion of Silk I in the whole crystal structure is not lower than 75%; the product can be directly dissolved in water at room temperature to form a stable clarified solution with the concentration not lower than 5%, and is not gelatinized after being stored at 4 DEG C for 90 days. The preparation method comprises the following steps: degumming and cleaning the mulberry silk raw material; the method comprises the following steps: carrying out short-time (less than or equal to 20 minutes) high-temperature dissolution by adopting a multi-element solution dissolution system with a specific ratio, and then carrying out dialysis purification at a low temperature by taking the conductivity (lt, 10 [mu] S / cm) of a dialysis external liquid and the concentration (lt, 50 ppm) of calcium ions as double end-point standards. The directional regulation and control of the silk fibroin structure are realized through the accurate control of the whole process, and the obtained product has excellent water solubility, storage stability and biological safety, and can be widely applied to the fields of drug delivery, 3D biological printing, medical beauty filling, tissue engineering and the like.