2229 results about "Biological safety" patented technology

The invention relates to serratia marcescens XJ108 and application thereof, and belongs to the technical field of microorganisms. The serratia marcescens XJ108 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.29557, the preservation date is January 10, 2024, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides application of the serratia marcescens XJ108 in acid environment, alkaline environment, high salinity environment and high chemical fertilizer environment soil tolerance and plant growth promotion. The serratia marcescens XJ108 provided by the invention has certain biological safety; meanwhile, the plant growth promoting function is achieved; and various extreme soil environments can be tolerated.

The invention relates to the field of traditional Chinese medicine effective component new dosage form pharmaceutical preparations, and discloses an M2 type macrophage membrane coated ROS responsive bionic nano drug delivery system, preparation and application. Mesoporous silica is used as a core to load a traditional Chinese medicine effective component, namely tripterine, and lipid components, namely PEGylated distearoyl phosphatidyl ethanolamine (DSPE-PEG), dioleoyl lecithin (DOPC), cholesterol and the like, are used as raw materials to prepare a ROS-responsive lipid coating, so that ROS-responsive tripterine mesoporous silica is formed. And then realizing bionic fusion with the M2 type macrophage membrane on the outer layer through an ultrasonic coating technology to prepare the M2 type macrophage membrane coated ROS responsive bionic mesoporous silica nano drug delivery system. The invention can realize targeted therapy of rheumatoid arthritis, and has the advantages of biological safety and the like.

The invention belongs to the technical field of biological medicines, and particularly discloses an epidermal growth factor-linker polypeptide-collagen, and the linker polypeptide has an amino acid sequence as shown in SEQ ID No.1. The linker polypeptide capable of connecting the EGF and the collagen is designed and synthesized, the N end of the linker polypeptide simulates an EGF receptor structure and can be combined with the EGF, and the linker polypeptide is connected with the EGF through non-covalent Van der Waals force, so that the structure of the EGF cannot be damaged; the C terminal of the linker polypeptide is rich in lysine residues, and can be covalently crosslinked to collagen through glutamine transaminase to form EGF-linker polypeptide-collagen. The protein solution taking EGF-linker polypeptide-collagen as active ingredients is low in cytotoxicity and high in biological safety, and meanwhile, the action time of the epidermal growth factor can be remarkably prolonged. In addition, a stable repairing microenvironment can be formed on the surface of the endometrium, and the endometrium injury repairing effect is remarkably improved.

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

The invention discloses an inflammation-targeting bionic extracellular vesicle as well as a preparation method and application thereof, and particularly relates to the field of medicines. According to the invention, a new generation of aminoglycoside antibiotic teicoplanin (Teic), which is high in albumin binding rate and weak in intracellular antibacterial effect, is loaded on neutrophil-derived extracellular vesicles (NEV) with natural drug carrying capacity and inflammatory response chemotactic capacity to prepare the drug-loaded vesicles (Teic-NEV), wherein the new generation of aminoglycoside antibiotic teicoplanin (Teic) is used for loading the neutrophil-derived extracellular vesicles (NEV). The drug-loaded extracellular vesicle Teic-NEV can be used for targeted therapy of cells and tissues infected by MRSA (Methicillin-Resistant Staphylococcus Aureus). In vitro, the Teic (at) NEV can be used for destroying MRSA mature biological membranes and killing intracellular MRSA. According to the invention, the in-vivo and in-vitro biological safety of the TeicatNEV is evaluated. Therefore, the invention provides a certain theoretical basis for the application of NEV-based drug delivery in the field of intracellular bacteria treatment, and also provides a new treatment strategy for in-vivo targeted treatment.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide for inhibiting drug-resistant staphylococcus aureus and application of the antibacterial peptide. The antibacterial peptide is obtained by performing N-terminal acetylation modification and C-terminal amidation modification on polypeptide as shown in SEQ ID NO.1. The invention further discloses a preparation method of the antibacterial peptide. The antibacterial peptide provided by the invention is a novel cationic antibacterial peptide, the minimum inhibitory concentrations of the antibacterial peptide to pathogenic bacteria staphylococcus aureus ATCC12600 and staphylococcus aureus ATCC33591 are respectively 4 [mu] g / mL and 32 [mu] g / mL, the biological safety is extremely high, and due to the characteristics, the antibacterial peptide shows extremely high medical drug antibacterial value, and can be used for preparing the antibacterial peptide. Alternative medicinal resources are provided for clinically resisting the two human pathogenic bacteria in the future.

The invention discloses a tissue adhesive based on mussel mucin as well as a preparation method and application thereof. The tissue adhesive is prepared from mussel mucin and an anionic compound containing a hydrophobic group, the mussel worm electrostatic self-assembly bionic composite material is developed on the basis of triple bionic principles of dopa adhesion of mussel byssus, electrostatic self-assembly polymerization of sandburg worms and hydrophobic acting force of barnacles. The preparation method of the tissue adhesive is simple, the tissue adhesive can be obtained only by mixing and oscillating the two stock solutions, the gel forming condition is simple and easy to implement, the pH range and the stock solution concentration range required by gel forming are large, the gel forming range is wide, the raw materials required in the preparation process are few, and the cost is low. Only two key raw materials including the mussel mucin and the anionic compound containing the hydrophobic group are needed, and the prepared tissue adhesive is high in adhesion speed and high in adhesion strength, has good cell compatibility and biological safety and can promote wound healing.

The invention discloses a titanium alloy feeding implant and a preparation method thereof, and the preparation method comprises the following steps: carrying out activating treatment on a biodegradable bonding system in an inert gas environment to form an expansion-state binder precursor, and then mixing titanium alloy powder and the precursor which are proportioned in stages in a multi-stage mixing device in stages to obtain the titanium alloy feeding implant. Sequentially executing low-temperature activation, melting and dispersing operations to prepare uniform feed; carrying out vibration-assisted injection molding on the feed to generate a green body with a multi-stage pore structure; removing the binder step by step and reserving pores by adopting a synergistic degreasing process of supercritical fluid extraction, microwave decomposition and gas phase cleaning; densification sintering molding of the titanium alloy implant is realized through pre-sintering and plasma activating treatment, so that an implant finished product is formed; according to the process, through the coupling design of innovation of a biodegradable binder system and an intelligent degreasing process, on the premise that the biological safety of the titanium alloy implant is ensured, precise forming and sintering densification control of a complex pore structure are synchronously achieved.

The invention provides a method for genetically modifying bordetella pertussis and application of the method. The method comprises the following steps: knocking down or knocking out a first nutrition screening gene of bordetella pertussis to generate a first auxotrophic cell; replacing the first genetic element with a second nutrition screening gene through recombination treatment to obtain a second auxotroph cell; replacing the second nutritional screening gene with the genetically modified second genetic element through recombination treatment to obtain a third nutritional deficient cell; and restoring the first nutritional screening gene to the genome of the third nutritional deficient cell through recombination treatment. In the modification process, use of antibiotics is not involved, no redundant fragments are introduced except for target modification sites, and the method has the advantages that the genetic background purity is high, and no biological safety risk exists in the modification process.

The invention discloses a cauliflower phenotype analysis and prediction method based on a field high-throughput phenotype system. According to the method, on the basis of RGB images and hyperspectral images of field cauliflower, through a cauliflower segmentation model, cauliflower, flower ball and leaf areas are automatically extracted, phenotype data and chlorophyll content are extracted, data of different growth periods are sorted according to a time sequence, a growth curve is drawn, and a regression model is constructed, so that the growth states of the cauliflower in different stages are predicted. Compared with the prior art, an effective non-contact scheme is provided for cauliflower phenotype analysis, phenotype parameters of cauliflower can be obtained without manual field on-site measurement, and corresponding culture measurement is combined, so that the cauliflower culture efficiency can be effectively improved, the culture cost can be reduced, and double friendliness of human resources and biological safety is realized.

The invention provides a tumor cell exosome, an anti-tumor immune drug delivery system and a preparation method and application of the anti-tumor immune drug delivery system, the tumor cell exosome is derived from starved and stimulated tumor cells, the tumor cell exosome is coated with nano drug particles for delivery, and the exosome has the effects of enhancing immune cell infiltration and remodeling an immune microenvironment; due to the homing effect of the exosome, the medicine and the exosome are more easily accumulated in the tumor, and the targeting property is improved; due to coating and camouflage of the biological membrane of the exosome membrane, the exosome membrane is prevented from being recognized, attacked and removed by an organism, and the biological safety is improved, so that the exosome and the nano-drug act on the tumor together to achieve a synergistic effect.

The invention provides a metal polyphenol network coated self-assembled nano-drug as well as a preparation method and application thereof, and belongs to the technical field of nano-drugs. The self-assembled nano-drug provided by the invention is of an organic-inorganic hybrid core-shell nano-structure; the core of the organic-inorganic hybrid core-shell nano structure is BC NPs; the BC NPs is formed by self-assembly of BBR (Boron Butadiene Rubber) and Cur (Carbon); a shell layer of the organic-inorganic hybrid core-shell nanostructure is a dynamic cross-linked network formed by self-assembly of metal ions and polyphenol through coordination. Metal ions and polyphenol are self-assembled through coordination to form a dynamic cross-linked network, and the dynamic cross-linked network coats the outer layer of the BC NPs nano-particles, so that the BC NPs nano-particles have good biocompatibility and biological safety; and the self-assembled nano-drug shows an obviously enhanced antibacterial effect under the combined treatment of NIR and H2O2.

The invention belongs to the cross technical field of nanomedicine, tumor treatment and immunotherapy, and particularly provides tumor mitochondria-targeted copper death selective induction nanoparticles, which are prepared by the following preparation method: S1, specifically coupling TPY and MHI through condensation reaction to obtain MTPY; s2, MTPY and copper ions are subjected to complexation, and an MTPY-Cu complex is obtained; and S3, the MTPY-Cu complex and albumin are subjected to self-assembly, and the MTPY-Cu-coated Alb nanoparticles are formed. The dosage of copper is only 1 / 1000 of that of free Cu < 2 + >, and equivalent immune regulation and killing effects can be achieved; primary tumors and distant tumor focuses can be inhibited, and lung metastasis is reduced; immune memory can be induced, and relapse can be prevented; the traditional Chinese medicine composition is combined with cis-platinum to remarkably enhance the curative effect and reverse the induced immunosuppression; the invention has the advantages of high biological safety and no obvious liver and kidney toxicity or hemolytic reaction.

The invention discloses a biphasic hydrogel scaffold of a bionic bone cartilage matrix as well as a preparation method and application of the biphasic hydrogel scaffold, and belongs to the technical field of tissue engineering. The biphasic hydrogel scaffold of the bionic bone cartilage matrix provided by the invention comprises a hard bone matrix phase and a cartilage matrix phase. Wherein hard chitosan hydrogel with a porous structure is used as a hard bone matrix phase, and soft chitosan hydrogel is used as a cartilage matrix phase. The biphase hydrogel with the asymmetric matrix stiffness and the biochemical microenvironment can effectively release active substances in a controlled mode, the release period exceeds 21 days, the time requirement for osteochondral repair is met, and lasting support is provided for proliferation and differentiation of cells. Meanwhile, the biphase hydrogel provided by the invention has good biological safety, two matrix phases have different mechanical properties, the biphase hydrogel adapts to in-vivo complex physiological and mechanical environments, the fate of stem cells can be directionally regulated and controlled, synchronous regeneration and repair of osteochondral tissues are realized, and the biphase hydrogel has a wide application prospect.

The invention relates to a multi-taste peptide design method based on deep learning, and belongs to the field of bioinformatics and food science and technology. Aiming at the technical problems that a traditional taste peptide development method is long in time consumption and low in efficiency and an existing calculation method is difficult to predict and generate multiple taste characteristic peptide fragments at the same time, the invention provides a solution based on a loss supervision adaptive variational auto-encoder (LA-VAE). According to the scheme, the standardized database is constructed by integrating multi-source data, model training is carried out by adopting a dual-mode training strategy and a dynamic loss supervision mechanism, and accurate control over various taste characteristics is achieved. 73 kinds of novel functional peptide fragments are successfully designed and verified by applying the method, and the peptide fragments show sweet taste, fresh taste and salty taste characteristics under different concentrations, have good biological safety and can be applied to the field of salt reduction and sugar reduction in the food industry.

The present invention relates to a molecular imaging probe for tumor diagnosis, and provides a sugar nucleic acid molecular imaging probe, the probe comprises a DNA aptamer sequence and more than one glycosyl nucleoside module, and is labeled by radionuclide, the DNA aptamer sequence can specifically target tumor cells after functional folding; the glycosyl nucleoside module is a glycosyl modified nucleoside derivative, is accurately modified at the 5'end or / and the 3 'end of the aptamer through DNA solid-phase synthesis, has the imaging characteristics of biological safety, high sensitivity and high resolution, and has an excellent diagnosis effect on part of cancers which are difficult to diagnose by a traditional method. The invention also provides a preparation method and a use method of the sugar nucleic acid molecular imaging probe, so that sugar modification of the aptamer is convenient, rapid, accurate, controllable and modularized, and the sugar nucleic acid molecular imaging probe has huge clinical application potential and transformation value in cancer diagnosis.

The invention discloses a biosecurity data interconnection and intercommunication method and system based on hybrid encryption. The method comprises the following steps: acquiring a minimum data set of biological safety monitoring of a patient; preprocessing the minimum data set to obtain a biological safety data packet; the biological safety data packet is encrypted to obtain an encryption result, and a secret key used for encryption is updated by adopting a dynamic management and regular alternation mechanism; integrating the encryption result, the hash value and the key information to obtain an integration result; and sending the integration result to a receiver, and performing verification, decryption and decompression by the receiver according to the integration result. By implementing the method provided by the invention, the security and privacy protection of the medical data in the collection, transmission and storage processes can be improved.

The preparation method comprises the following steps: synthesizing gold nanostars (GNS) by using a seed growth method, coating mesoporous cerium dioxide (mCeO2) by using a template method, and loading a photosensitizer indocyanine green (ICG), so as to form a nano enzyme diagnosis and treatment system with chemical activity, photoactivity and photothermal effects. In the system, the gold nanostars have a good photo-thermal effect due to the multi-branched structure of the gold nanostars; on one hand, the mesoporous cerium dioxide coated on the outer layer has a chemical effect due to the nano-enzyme effect, and on the other hand, the mesoporous structure of the mesoporous cerium dioxide can better load the photosensitizer indocyanine green. All the components have good stability and biological safety, also have peroxidase simulation activity and a drug loading function, can improve the level of reactive oxygen species (ROS) in a tumor microenvironment and load photosensitizer drugs, and have an excellent anti-tumor effect and small side effects.

The invention discloses a preparation method of a bionic cornea stroma lens for refractive correction, which comprises the following steps: solution preparation: dissolving type I collagen in an acetic acid solution, adding a film aid, fully stirring, and centrifugally defoaming to obtain a type I collagen composite solution; the I-type collagen composite solution is poured into an assembling groove, the curvature radius of titanium metal is set to be 6.8-8.0 mm, the titanium metal serves as a working electrode material, platinum metal serves as a counter electrode material, current parameters are set according to the needed thickness of the lens blank, the density is 3.50-6.50 mA / cm < 2 >, and the thickness of the lens blank is set to be 3.50-6.50 mA / cm < 2 >; a high-transparency cornea embryo material which is uniform in curvature in all directions and similar to human cornea fiber structure arrangement is formed after electrochemical assembly and cross-linking curing, accurate cutting machining is carried out through excimer laser, a concave / convex matrix lens with the needed correction degree is obtained, the correction precision is high after cornea interlayer implantation, and the correction precision is high. And meanwhile, the compound has excellent performance in the aspects of in-vivo stability and biological safety.

The invention discloses nanoparticles for a contrast agent as well as a preparation method and application of the nanoparticles, and relates to the technical field of nano composite materials. According to the nanoparticle for the contrast agent, a shell layer comprises polydopamine and albumin which are connected through a covalent bond, a core structure comprises a complex formed by metal ions and polyphenol, and the metal ions in the core structure and the polydopamine in the shell layer also have a coordination effect. Due to the fact that all the components are tightly connected, the rotation time of the nano-particles is prolonged, the T1 relaxation rate of the nano-particles is greatly increased, meanwhile, due to introduction of polydopamine, release of metal ions in a human body is avoided, the biological safety of the nano-particles is improved, albumin in the nano-particles can further improve the biocompatibility of the particles, and the nano-particles can be used for preparing the nano-particles. Therefore, the nanoparticles provided by the invention also have wide application prospects in the fields of fluorescence imaging and targeted drug delivery.

The invention belongs to the technical field of medicines, cosmetics and scalp care, and particularly relates to application of coniferaldehydes and derivatives or salts of the coniferaldehydes to preparation of medicines and cosmetics for promoting hair regeneration and treating chemotherapy-induced alopecia (CIA, CIA) and other types of alopecia, in particular to application of the coniferaldehydes and the derivatives or salts of the coniferaldehydes to preparation of the medicines and the cosmetics. Experimental results show that coniferyl aldehyde can promote hair regeneration of mice, accelerate hair growth of the mice and effectively relieve alopecia symptoms of the mice after chemotherapy, and the effect of coniferyl aldehyde is better than that of minoxidil. According to the application, coniferyl aldehyde or a composition thereof is given to a subject, so that the vigor of hair follicles is remarkably enhanced, the stationary period of the hair follicles is shortened, and the hair loss process is inhibited. The coniferyl aldehyde provided by the invention has the industrial application advantages of reliable biological safety, controllable preparation cost and high raw material accessibility, can be obtained by a plant extraction or chemical synthesis method, is suitable for an external solution, gel or a scalp care preparation, and provides a natural, safe and effective solution for alopecia patients.

The invention discloses a nano-antibacterial agent for treating biofilm infection through Prussian blue and a preparation method and application thereof.According to the nano-antibacterial agent, hollow Prussian blue (HPB) serves as a carrier to load a substance with anti-inflammatory and anti-oxidation effects, a metal polyphenol network structure is formed through chelating of tannic acid (TA) modified by hyaluronic acid (HA) and high-valence metal ions, and the nano-antibacterial agent is used for treating biofilm infection through Prussian blue. And the MPNs are packaged to obtain the product. All the components of the nano antibacterial agent have a synergistic effect, MPNs formed by chelating TA modified by hyaluronic acid and high-valence metal ions in the antibacterial agent have good photo-thermal performance, and light energy can be converted into heat energy for photo-thermal therapy. According to the invention, the nanoparticles for photo-thermal antisepsis and anti-inflammation are constructed, so that comprehensive treatment of anti-biofilm and anti-inflammatory effect is realized, and healing of infected parts is promoted. And the antibacterial agent has good biocompatibility and high biological safety, and is very suitable for replacing antibiotics to treat biological membrane infection.

The invention provides a polypeptide which is derived from zebra fish antibacterial peptide and is improved in heat resistance. The amino acid sequence of the polypeptide AR10 is WKRKRWRIWK, and the polypeptide AR10 has higher antibacterial activity than the unmodified polypeptide AR10, and has better high temperature resistance, salt resistance and serum resistance. The antibacterial peptide provided by the invention not only has a good antibacterial effect on common Gram-positive and Gram-negative pathogenic bacteria, but also has a good antibacterial effect on clinically common drug-resistant bacteria, and cell experiment results show that the screened antibacterial peptide has biological safety. Therefore, the antibacterial peptide provided by the invention has broad-spectrum antibacterial property and high biological safety, and can be used for developing antibacterial and bacteriostatic products related to human bodies.

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention belongs to the field of nano-enzyme preparation and biological application, and particularly relates to a preparation method and application of M2 type macrophage membrane coated FeMn diatomic nano-enzyme. The preparation method comprises the following steps: by taking nitrogen-doped carbon (BANT) as a carrier, loading Fe and Mn diatoms, synthesizing a novel nano material FeMnDA / BCNT diatomic nano-enzyme, extracting a macrophage membrane from natural macrophages, and coating the FeMnDA / BCNT nano material with the macrophage membrane to finally form M2 type macrophage membrane coated nano-particles, namely [MM] FeMnDA / BCNT. The [MM] FeMnDA / BCNT nano-enzyme prepared by the invention has good biological safety and simulated SOD and CAT enzyme activity, and the expression of inflammatory factors is reduced by removing excessive ROS (reactive oxygen species) in the cartilage cells induced by H2O2, so that the damage of oxidative stress to the cartilage cells is inhibited. The [MM] FeMnDA / BCNT nano-enzyme is not used for treating OA yet, so that a scientific basis is provided for further application and expansion of the diatomic nano-enzyme, and an effective strategy and a new thought are provided for treating osteoarthritis and chronic diseases related to oxidative stress in the future.

The invention discloses a rare earth antibacterial ceramic and a preparation method thereof, and belongs to the technical field of ceramic materials, the rare earth antibacterial ceramic comprises a base material, a rare earth antibacterial agent and an auxiliary additive, the base material is coated with a zirconium silicate coating layer; the matrix material is one of aluminum oxide or zirconium oxide, the rare earth antibacterial agent is a ternary composite oxide composed of at least three lanthanide elements, the lanthanide elements comprise Ce, Y, La and Nd, and the invention provides a preparation method of the composite material. Through collaborative innovation of ternary rare earth composite modification, zirconium silicate interface coating and a gradient sintering process, the core problems of single antibacterial spectrum, high-temperature failure and insufficient durability of traditional antibacterial ceramic are solved, and the antibacterial property, stability, biological safety and mechanical property are remarkably improved; and the method has clear technical progress and industrial application value.

The invention discloses a yolk protein source tripeptide with a function of improving osteoporosis and application of the yolk protein source tripeptide, and belongs to the technical field of biological medicines. The tripeptide is a tripeptide APW of which the amino acid sequence is Ala-Pro-Trp. The peptide fragment can be obtained through artificial synthesis or directional enzymolysis of defatted yolk powder. Functional verification shows that the tripeptide APW and the egg yolk protein hydrolysate containing the peptide fragment have the function of improving the bone mineral density, and in a zebra fish osteoporosis model, when the egg yolk protein hydrolysate is 20 mu g / mL, the bone mineral density is improved by 58%; when the tripeptide APW is 0.5 mu g / mL, the bone mineral density is increased by 23.4%; in a rat osteoporosis model, the bone mineral density of thighbone is increased by 3.39% after 10 mg / kg. D of egg yolk protein enzymatic hydrolysate is eaten. Moreover, the food-borne bioactive peptide is high in biological safety, so that the tripeptide APW and a yolk protein enzymolysis product containing the peptide fragment can be applied to preparation of medicines for treating osteoporosis or health-care foods for improving bone mineral density, and the tripeptide APW has a good market prospect and application potential.

The invention provides an intelligent ventilation control method and system for a biological laboratory. The method comprises the steps that if it is monitored that the operation state of an air exhaust system is changed into a shutdown state, first working parameters of a ventilation system of the biological laboratory are determined according to shutdown parameters of the air exhaust system, and the ventilation system is controlled to execute weak air exhaust operation according to the first working parameters; shooting high-definition image data of an operation area of the biological safety cabinet, extracting biological experiment data from the high-definition image data, predicting the generation amount of harmful gas residues according to the biological experiment data, and determining a second working parameter of the ventilation system according to the generation amount of the harmful gas residues, and the ventilation system is controlled to execute strong exhaust operation according to the second working parameter. According to the scheme, after the air exhaust system of the biological safety cabinet is shut down, harmful gas in the biological laboratory can be exhausted by regulating and controlling the ventilation system in the biological laboratory, and it is ensured that a healthy air environment is kept in the biological laboratory all the time.

The invention discloses a nanometer delivery device for a gastric cancer targeted drug and a use method of the nanometer delivery device, and belongs to the technical field of drug targeted delivery. The device comprises a molecular recognition and optimization module which is responsible for accurately recognizing a tumor cell specific molecular marker and providing personalized treatment strategy recommendation; the nano-carrier design module is used for designing multifunctional lipid-polymer hybrid nano-particles and carrying out surface targeting ligand modification; the intelligent drug loading module is used for improving the stability and controllability of drug loading based on a pH / temperature sensitive release mechanism and effectively reducing the toxic influence of non-targeted tissues; the molecular imaging and monitoring module integrates a nanoprobe imaging technology and a quantum dot tracing technology, constructs a treatment-imaging integrated system, realizes real-time dynamic monitoring of a treatment process, and provides instant feedback of a molecular level; and the risk assessment module is used for establishing a safety database and providing dynamic risk assessment of the treatment process through a biological safety assessment system and long-term tracking management.

The invention discloses an eel antibacterial polypeptide Ajaspkidin and application thereof in preparation of an anti-vibrio composition. The amino acid sequence of the eel antibacterial polypeptide Ajaspkidin is shown as SEQ ID NO.01. The invention further discloses an application of the eel antibacterial polypeptide The zebra fish antibacterial peptide has the characteristics of excellent molecular characteristics, broad-spectrum antibacterial activity, efficient bactericidal ability, high thermal stability, high biological safety, anti-infection effect on zebra fish infected by vibrio vulnificus, capability of remarkably improving the survival rate of bred animals and the like, and has a wide application prospect.